CN105695376A - Bacillus simplex for poisoning plant root-knot nematodes and application of bacillus simplex - Google Patents
Bacillus simplex for poisoning plant root-knot nematodes and application of bacillus simplex Download PDFInfo
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- CN105695376A CN105695376A CN201610262463.4A CN201610262463A CN105695376A CN 105695376 A CN105695376 A CN 105695376A CN 201610262463 A CN201610262463 A CN 201610262463A CN 105695376 A CN105695376 A CN 105695376A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
Abstract
The invention discloses bacillus simplex for poisoning plant root-knot nematodes and application of the bacillus simplex. The strain number of the bacillus simplex is JZB128, and the registering serial number of the bacillus simplex in the China General Microbiological Culture Collection Center is CGMCC No.12345.The corrected death rate of the bacillus simplex JZB128 on meloidogyn incognita is 96.8%, the corrected death rate of the bacillus simplex JZB128 on the meloidogyn incognita is 95.2% after the bacillus simplex JZB128 is continuously cultured for four generations, and the average inhibition rate of the bacillus simplex JZB128 on meloidogyn incognita egg hatching is 96.0%.On the condition that no auxiliary is added, the prevention effect on a cucumber root-knot nematode disease at the 45<th> day reaches 77.7%, and prevention effect on the cucumber root-knot nematode disease after 80 days still reaches 62.4%.The bacillus simplex JZB128 and/or metabolites of the bacillus simplex JZB128 can be applied to prevention and control over the plant root-knot nematodes.
Description
Technical field
The invention belongs to microbial pesticide technical field, be specifically related to simple bacillus cereus and the application thereof of a strain poisoning plant root-knot nematode。
Background technology
The class soil-borne disease that root-knot nematode Meloidogynespp. is serious harm agricultural production is former, multiple kinds of crops can be parasitized, especially vegetable, Severe Reduction (KiewnickS is caused in subtropical and tropical zones, SikoraRA.2006.Biologicalcontroloftheroot-knotnematodeMel oidogyneincognitabyPaecilomyceslilacinusstrain251.Biolog icalControl, 38 (2): 179-187.)。In recent years, along with China's growing vegetables area constantly expands, the generation of root knot nematode disease increases the weight of year by year, become have a strong impact on victual safety one of difficult disease (structure of the .2010. greenhouse cucumber root-knot nematode spot grand genomic library of soil microorganism such as Zhao Zhixiang and the screening of one nematicide protease gene. microorganism journal, 50 (8): 1072-1079)。
Current control of nematode is still based on nematocide preventing and treating, owing to it is to the toxicity of non-target organism with to people and animals' severe toxicity, serious environment pollution and underground water source, and the residual contamination of pesticide directly affects quality and the quality of agricultural product, the harm of nematicide has become restriction pollution-free vegetable and has produced sustainable development and the outstanding problem of food safety guarantee。Therefore, develop environmentally friendly, the GR that people, animal are nontoxic is extremely urgent。
The bacteria growth of bacillus is fast, fertility is strong, nutritional need is simple, there are stronger thermostability and resistance, it is prone to survival and breeding at plant surface, to person poultry harmless, free from environmental pollution, preparation stabilization, uses conveniently, meet people to the requirement of pollution-free food (the .2012. bacillus cereus Biological control research of plant disease progress such as Zhu Yan. Agriculture of Anhui science, 40 (34): 16635-16638)。
Summary of the invention
The technical problem to be solved is how to prevent and treat plant root-knot nematode。
For solving above technical problem, the invention provides a simple bacillus cereus of strain。
Simple bacillus cereus provided by the present invention, its bacterial strain number is JZB128, and it is numbered CGMCCNo.12345 in registering on the books of China Committee for Culture Collection of Microorganisms's common micro-organisms center, hereinafter referred to as simple bacillus cereus JZB128。
Above-mentioned simple bacillus cereus JZB128 can spore, mycelia or the mycelial form containing spore and/or mycelia exist。
The culture of above-mentioned simple bacillus cereus JZB128 falls within protection scope of the present invention。
The culture of simple bacillus cereus JZB128 provided by the present invention, being that simple bacillus cereus JZB128 is cultivated the material in the culture vessel obtained in microbiological culture media, described material includes described simple bacillus cereus and the metabolite of described simple bacillus cereus。
In the culture of above-mentioned simple bacillus cereus JZB128, described microbiological culture media can be solid medium or fluid medium。
For solving above technical problem, the invention provides plant root-knot nematode inhibitor。
Plant root-knot nematode inhibitor provided by the present invention, its active component is simple bacillus cereus JZB128 and/or the metabolite of simple bacillus cereus JZB128。
The metabolite of above-mentioned simple bacillus cereus JZB128 can obtain from the fermentation liquid of described simple bacillus cereus JZB128。The metabolite of described simple bacillus cereus JZB128 specifically can be prepared as follows, liquid medium within is cultivated described simple bacillus cereus JZB128, removes the simple bacillus cereus JZB128 in liquid culture (fermentation liquid) and namely obtain the metabolite of described simple bacillus cereus JZB128。
In above-mentioned plant root-knot nematode inhibitor, described root-knot nematode can be Meloidogyne incognita。
The application in preparing plant root-knot nematode inhibitor of the metabolite of above-mentioned simple bacillus cereus JZB128 and/or simple bacillus cereus JZB128 and/or the culture of simple bacillus cereus JZB128 or the application in preparation suppression plant root-knot nematode harm Fructus Cucumidis sativi medicament fall within protection scope of the present invention。
The application in suppressing plant root-knot nematode of the metabolite of above-mentioned simple bacillus cereus JZB128 and/or simple bacillus cereus JZB128 and/or the culture of simple bacillus cereus JZB128 or the application in suppression plant root-knot nematode harm Fructus Cucumidis sativi fall within protection scope of the present invention。
In above-mentioned application, described root-knot nematode can be Meloidogyne incognita。
Above, in described plant root-knot nematode inhibitor, except described active component, possibly together with carrier。Described carrier can for pesticide field conventional and at the carrier being biologically inertia。Described carrier can be solid carrier or liquid-carrier；Described solid carrier can be mineral material, vegetable material or macromolecular compound；Described mineral material can be at least one in clay, Talcum, Kaolin, montmorillonite, white carbon, zeolite, Silicon stone and kieselguhr；Described vegetable material can be at least one in Semen Maydis powder, Semen Glycines powder and starch；Described macromolecular compound can be polyvinyl alcohol and/or polyglycols；Described liquid-carrier can be organic solvent, vegetable oil, mineral oil or water；Described organic solvent can be decane and/or dodecane。
In above-mentioned plant root-knot nematode inhibitor, simple bacillus cereus JZB128 can spore, mycelia or the mycelial form containing spore and/or mycelia exist。
The dosage form of described plant root-knot nematode inhibitor can be multiple dosage form, such as liquor, Emulsion, suspending agent, powder, granule, wettable powder or water dispersible granules。
As required, described plant root-knot nematode inhibitor also can add surfactant (such as polysorbas20, Tween 80 etc.), binding agent, stabilizer (such as antioxidant), pH adjusting agent etc.。
Experiment proves, the corrected mortality of Meloidogyne incognita is 96.8% by simple bacillus cereus (Bacillussimplex) JZB128, after simple bacillus cereus (Bacillussimplex) JZB128 bacterial strain cultivated for 4 generations continuously, the corrected mortality to Meloidogyne incognita is 95.2%。The average inhibition of Meloidogyne incognita egg hatching is 96.0% by simple bacillus cereus (Bacillussimplex) JZB128。Cucumber root-knot nematode disease preventive effect, when not having any auxiliary agent to dose, is reached 77.7% when 45 days, after 80 days, Cucumber root-knot nematode disease preventive effect is still reached 62.4% by simple bacillus cereus (Bacillussimplex) JZB128 liquid fermentation and culture thing。Cucumber seedling well developed root system after the culture of simple bacillus cereus JZB128 or simple bacillus cereus JZB128 processes, root knot is few, and plant strain growth is normal, to Fructus Cucumidis sativi safety。The metabolite of simple bacillus cereus JZB128 and/or simple bacillus cereus JZB128 can be used in the preventing and treating of plant root-knot nematode。
Preservation explanation
Strain name: simple bacillus cereus (Bacillussimplex)
Strain number: JZB128
Preservation mechanism: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation mechanism is called for short: CGMCC
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Preservation date: on April 12nd, 2016
Register on the books numbering: CGMCCNo.12345 at preservation center
Accompanying drawing explanation
Fig. 1 is bacterial strain JZB128 colonial morphology on beef extract-peptone flat board。
Fig. 2 is bacterial strain JZB128 killing root-knot nematode hereditary stability
Cucumber root photo when Fig. 3 is matched group dispenser 45 days。
Cucumber root photo when Fig. 4 is JZB128 process group dispenser 45 days。
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention being further described in detail, the embodiment provided is only for illustrating the present invention, rather than in order to limit the scope of the present invention。Experimental technique in following embodiment, if no special instructions, is conventional method。Material used in following embodiment, reagent etc., if no special instructions, all commercially obtain。
Embodiment 1, the separation of simple bacillus cereus JZB128 and qualification
1.1 strains separation
In November, 2014 is green Fructus Lycopersici esculenti plot, booth base difficult to understand in In Shunyi District of Beijing, with sampler, (length is 55cm, diameter is 2cm) take the soil that nematicide Fructus Lycopersici esculenti (kind: the hard powder 8 of Holland) is deep for rhizosphere 10cm, take 5 points at random, take 5 parts of soil samples altogether, gained soil sample is mixed, loads in valve bag, take back laboratory treatment。Weighing soil sample 2g, be equipped with in the conical flask of 18mL sterilized water, 220r/min shakes 30min。It is made into 10-2-10-6Graded series soil supension。Drawing different gradient soil supension 200 μ L respectively, be uniformly coated onto on beef extract-peptone flat board, each concentration repeats 3 times。It is positioned in 28 DEG C of incubators and cultivates, after 2-5d, according to surface character such as the color of bacterium colony, shape, edge, transparency, glossiness, choose single bacterium colony, the flat lining out of beef-protein medium 3 times, it is thus achieved that pure culture, after length is good, it is placed in refrigerator and preserves。Take the bacterial strain being numbered JZB128 and carry out following qualification。
1.2 identification of strains
1.2.1 strain morphology is observed
1. apparent qualification: by observing the color etc. of the color of thalline, size, form, edge, transparency, glossiness, culture medium, and observe individual morphology feature by Gram’s staining。
By JZB128 on beef-protein medium flat board streak culture, as can see from Figure 1: JZB128 bacterium colony canescence, opaque, have that fold, bacterium colony be glossy, edge is slightly neat, microscopy finds that thalline is shaft-like, Gram’s staining is positive, spore, above-mentioned morphological characteristic is had to show that bacterial strain JZB128 belongs to bacillus。
2. physiological and biochemical test: adopt the bio-chemical characteristics such as nitrate reduction experiment, salt tolerance cultivation, gelatin liquefaction, Starch Hydrolysis experiment, sodium citrate salt。
JZB128 part bio-chemical characteristics result such as table 1。It is shown that bacterial isolates JZB128 physiological and biochemical property and bacillus physiological and biochemical property basic simlarity。
Table 1JZB128 part bio-chemical characteristics
| Bio-chemical characteristics testing index | JZB128 |
| Optimum temperature (DEG C) | 28 |
| Gelatin liquefaction | + |
| Starch Hydrolysis | + |
| Nitrate reductase | + |
| Hydrogen sulfide produces | + |
| Cellulose hydrolysis | - |
| Citrate utilizes reaction | - |
| C.I. 13020. is tested | - |
| Tyrosine reacts | - |
Note: "-" represents negative, "+" represent the positive
3. Molecular Identification: RNA isolation kit extracts bacterial isolates genomic DNA。With the DNA that extracts for template, amplimer is universal primer 27F (5 '-GAGAGTTTGATCCTGGCTCAG-3 ') and 1492R (5 '-AAGGAGGTGATCCAGCCGCA-3 ')。PCR reaction condition is: 94 DEG C of denaturation 5min；94 DEG C of 1min, 56 DEG C of 30s, 72 DEG C of 1min, 30 circulations；72 DEG C of 10min, 4 DEG C of insulations。PCR product detects through 1% agarose gel electrophoresis, detect qualified after send raw work biological engineering (Beijing) company limited to check order。
The 16SrDNA sequence (in sequence table sequence 1) of bacterial strain JZB128 is obtained through order-checking, analyzed by Blast, choose the bacterial strain that homology with it is higher, utilize software MEGA5.0 to carry out the 16SrDNA phylogenetic tree of Phylogenetic Analysis, Neighbor-Joining method structure bacterial strain。Result shows that JZB128 and Bacillussimplex gathers in a branch, and homology is 100%, in conjunction with before morphological characteristic and bio-chemical characteristics, JZB128 is accredited as simple bacillus cereus。
Simple bacillus cereus (Bacillussimplex) JZB128 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 12nd, 2016, and preserving number is CGMCCNo.12345。
Embodiment 2, plant root-knot nematode inhibitor and eelworm-killing activity thereof
1, the cultivation of simple bacillus cereus (Bacillussimplex) JZB128
Beef-protein medium: Carnis Bovis seu Bubali cream 3.0g, peptone 10.0g, NaCl5.0g, agar 15.0g, distilled water is settled to 1000ml, mixes subpackage, 121 DEG C of sterilizing 20min。By simple bacillus cereus (Bacillussimplex) JZB128 the flat lining out of beef-protein medium 3 times, it is thus achieved that single bacterium colony。
2. the eelworm-killing activity of simple bacillus cereus (Bacillussimplex) JZB128
The acquisition of 2.1 Meloidogyne incognita ovum, egg capsule and second instar larvae
Meloidogyne incognita No. 15 indoor pot vaccination ways of the good powder of susceptible tomato variety preserve。After inoculation 40d, having a large amount of egg capsule to occur at Tomato Root System, rinsed gently by root system water, carefully take off egg capsule, be placed in 0.5% liquor natrii hypochloritis to sterilize 3min, then with aseptic water washing 3 times, be placed in the culture dish filling a small amount of sterilized water, 4 DEG C save backup。
Separately taking part egg capsule and be placed in the culture dish filling a small amount of sterilized water, cultivate 4d for 25 DEG C, collect once the Meloidogyne incognita second instar larvae of new hatching every 24h, room temperature preservation is standby。
Old complaint is cleaned, is cut into the segment of 0.5-1cm, put in 500mL triangular flask, add 1% liquor natrii hypochloritis 200mL, acutely shake 5min, repeatedly rinse collection with 200 orders and 500 order mesh screens, collecting pure Meloidogyne incognita ovum by 500 order mesh screens, 4 DEG C save backup。
2.2 simple bacillus cereus (Bacillussimplex) JZB128 Activity determination to Meloidogyne incognita
2.2.132 well plate method Activity determination
Experiment sets following two process:
JZB128 process group: simple bacillus cereus (Bacillussimplex) JZB128 is connected in the 50mL triangular flask equipped with 20mL beef extract-peptone fluid medium, 28 DEG C, cultivate 48h under 220r/min and collect fermentation liquid, in this fermentation liquid, the content of simple bacillus cereus (Bacillussimplex) JZB128 is 2 × 1010Cfu/mL。With the water system membrane filtration fermentation liquid of 0.22 μm, collecting filtrate, filtrate added in the cavities of 32 orifice plate culture plates, every hole adds 100 μ L, adds isopyknic Meloidogyne incognita second instar larvae solution (200/mL), and each process is repeated 4 times。After 28 DEG C of incubators cultivate 24h, under stereomicroscope, observe and record nematicide death toll, calculate corrected mortality。This experimental implementation carries out in an aseptic environment。
Matched group: substitute in the cavities that the filtrate in JZB128 process group adds 32 orifice plate culture plates with beef extract-peptone fluid medium, every hole adds 100 μ L, adding isopyknic Meloidogyne incognita second instar larvae solution (200/mL), each process is repeated 4 times。After 28 DEG C of incubators cultivate 24h, under stereomicroscope, observe and record nematicide death toll, calculate corrected mortality。This experimental implementation carries out in an aseptic environment。
2.2.2 bacterial strain hereditary stability detection
By simple bacillus cereus (Bacillussimplex) JZB128 continuous 4 line successive transfer culture on beef-protein medium flat board。Detect the anti-root-knot nematode activity of every generation bacterial strain。Calculate the corrected mortality of every generation bacterial strain killing root-knot nematode。The same 2.2.1 of method。
2.2.3 the inhibitory action to the hatching of Meloidogyne incognita egg capsule
Experiment sets following two process:
JZB128 process group: simple bacillus cereus (Bacillussimplex) JZB128 is connected in the 50mL triangular flask equipped with 20mL beef extract-peptone fluid medium, 28 DEG C, cultivate 48h under 220r/min and collect fermentation liquid, in this fermentation liquid, the content of simple bacillus cereus (Bacillussimplex) JZB128 is 2 × 1010Cfu/mL。With the water system membrane filtration fermentation liquid of 0.22 μm, collecting filtrate, filtrate added in the cavities of 24 orifice plate culture plates, every hole adds 900 μ L；Taking fresh full Meloidogyne incognita egg capsule, size is basically identical, 0.5% liquor natrii hypochloritis surface sterilization 2min, and aseptic water washing 3 times puts into the cavities equipped with filtrate to be measured, and each cavities puts 2 egg capsules。Each process is repeated 3 times, hatches at 25 DEG C。The quantity of the nematicide that statistics hatches is observed after 5d。Calculate the relative inhibition of line eggs hatching。
Matched group: substituting in the cavities that the filtrate in JZB128 process group adds 24 orifice plate culture plates with beef extract-peptone fluid medium, every hole adds 900 μ L；Taking fresh full Meloidogyne incognita egg capsule, size is basically identical, 0.5% liquor natrii hypochloritis surface sterilization 2min, and aseptic water washing 3 times puts into the cavities equipped with filtrate to be measured, and each cavities puts 2 egg capsules。Each process is repeated 3 times, hatches at 25 DEG C。The quantity of the nematicide that statistics hatches is observed after 5d。Calculate the relative inhibition of line eggs hatching。
Relative inhibition (%)=100 × (matched group hatching nematicide average-process group hatching nematicide average) ÷ matched group hatching nematicide average。
Eelworm-killing activity experimental result: the corrected mortality of Meloidogyne incognita is 96.8% by simple bacillus cereus (Bacillussimplex) JZB128, after simple bacillus cereus (Bacillussimplex) JZB128 cultivated for 4 generations continuously, the corrected mortality to Meloidogyne incognita is 95.2% (Fig. 2)。To sum up analyzing, simple bacillus cereus (Bacillussimplex) JZB128 has stronger and stable killing root-knot nematode activity。Simple bacillus cereus (Bacillussimplex) JZB128 fermentation liquid suppresses root-knot nematode egg hatching experiment it is shown that the average inhibition of Meloidogyne incognita egg hatching is 96.0% (result is in Table 2) by JZB128。
The impact on root-knot nematode egg hatching of the table 2JZB128 fermentation liquid
Inhibitory action result of the test Meloidogyne incognita vigor and egg capsule hatched by both the above is shown, simple bacillus cereus (Bacillussimplex) JZB128 is the antibacterial that a strain has using value, particularly to the nematocidal effect of Meloidogyne incognita and inhibitory action that its egg capsule is hatched, it is shown that its good application and development prospect。
2.3 greenhouse pot culture preventing and treating Fructus Cucumidis sativi Meloidogyne incognita (Meloidogyneincognita) effects
Material to be tested and method:
Fructus Cucumidis sativi: kind is Beijing 402
Nematicide: Meloidogyne incognita Meloidogyneincognita
Cucumber seedling-raising in diameter 13cm, high 10cm nutritive cube in, nursery soil is mix in 1:4 ratio without nematicide garden mould and sandy soil。After emerging, each nutritive cube of thinning stays 1 strain。
JZB128 liquid fermentation and culture: fermentation medium is that (solvent is water to LB fluid medium, solute and concentration thereof are tryptone (Tryptone) 10g/L, yeast extract (Yeastextract) 5g/L, sodium chloride (NaCl) 10g/L, adjust pH to 7.0-7.2,121 DEG C of sterilizing 20min)。The mono-colony inoculation of picking JZB128 to equipped with in the 500mL triangular flask of 100mLLB fluid medium, 28 DEG C, shaking speed 220r min-1, fermentation 48h, obtain simple bacillus cereus (Bacillussimplex) JZB128 fermentation liquid, in this simple bacillus cereus (Bacillussimplex) JZB128 fermentation liquid, the content of simple bacillus cereus (Bacillussimplex) JZB128 is 2 × 1010cfu/mL。
Experiment sets following two process:
JZB128 process group: inoculate Meloidogyne incognita after waiting cucumber seedling to grow 4-5 sheet true leaf: cultured Meloidogyne incognita is configured to the nematicide suspension of 100/mL, plant root evenly around insert 3 apertures, nematicide suspension is instilled in aperture, every nutritive cube 1500 Meloidogyne incognitas of inoculation。Carrying out chemicals treatment the same day, simple bacillus cereus (Bacillussimplex) JZB128 fermentation liquid consumption is 1/5 (amount of liquid quality/soil sample weight) with the native ratio in nutritive cube。Experiment sets 3 repetitions, repeats to process 10 strain Fructus Cucumidiss sativi every time。It is then placed within greenhouse to cultivate。Reprocess once (consumption same last time) with simple bacillus cereus (Bacillussimplex) JZB128 fermentation liquid after cultivating 10 days, normal field management, after administration 45 days and 80 days investigation root knot numbers。Prevention effect is determined according to root knot number。
Matched group: simple bacillus cereus (Bacillussimplex) JZB128 fermentation liquid replaces with the clear water of equivalent, and other experimental implementation is identical with JZB128 process group。
Simple bacillus cereus (Bacillussimplex) the JZB128 fermentation liquid greenhouse pot culture result of the test of table 3
Result shows, simple bacillus cereus (Bacillussimplex) JZB128 liquid fermentation and culture thing is not when having any auxiliary agent to dose, use 2 times, when 45 days, Cucumber root-knot nematode disease preventive effect is reached 77.7%, after 80 days, Cucumber root-knot nematode disease preventive effect is still reached 62.4% (table 3)。Cucumber seedling well developed root system after simple bacillus cereus (Bacillussimplex) JZB128 processes, root knot is few, plant strain growth is normal, do not find obvious poisoning (Fig. 3 and Fig. 4), to Fructus Cucumidis sativi safety, having good using value, this concentration has reached the concentration requirement that industrialization development utilizes。
Claims (9)
1. simple bacillus cereus, its bacterial strain number is JZB128, and it is numbered CGMCCNo.12345 in registering on the books of China Committee for Culture Collection of Microorganisms's common micro-organisms center。
2. the culture of simple bacillus cereus described in claim 1, is that the simple bacillus cereus described in claim 1 is cultivated the material in the culture vessel obtained in microbiological culture media。
3. plant root-knot nematode inhibitor, its active component is the metabolite of the simple bacillus cereus described in claim 1 and/or the simple bacillus cereus described in claim 1。
4. plant root-knot nematode inhibitor according to claim 3, it is characterised in that: described root-knot nematode is Meloidogyne incognita。
5. any one purposes following of the metabolite of the simple bacillus cereus described in claim 1 and/or the simple bacillus cereus described in claim 1:
A1) application in preparing plant root-knot nematode inhibitor；
A2) application in plant nematode harm Fructus Cucumidis sativi medicament is suppressed in preparation。
6. any one purposes following of the culture described in claim 2:
A1) application in preparing plant root-knot nematode inhibitor；
A2) application in plant nematode harm Fructus Cucumidis sativi medicament is suppressed in preparation。
7. any one purposes following of the metabolite of the simple bacillus cereus described in claim 1 and/or the simple bacillus cereus described in claim 1:
B1) application in suppressing plant root-knot nematode；
B2) application in suppressing plant root-knot nematode harm Fructus Cucumidis sativi。
8. any one purposes following of the culture described in claim 2:
B1) application in suppressing plant root-knot nematode；
B2) application in suppressing plant root-knot nematode harm Fructus Cucumidis sativi。
9. according to described application arbitrary in claim 5-8, it is characterised in that: described root-knot nematode is Meloidogyne incognita。
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108531430A (en) * | 2018-05-09 | 2018-09-14 | 青海省农林科学院 | A kind of simple bacillus S62 and its application |
| CN113248308A (en) * | 2021-05-20 | 2021-08-13 | 施可丰化工股份有限公司 | Microbial agent for effectively preventing and treating root knot nematode disease and preparation method thereof |
| CN113785765A (en) * | 2021-09-15 | 2021-12-14 | 中国农业大学 | Kit for visually researching interaction relation between plant and nematode, method and application |
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| CN105002121A (en) * | 2015-08-14 | 2015-10-28 | 山东泰诺药业有限公司 | Bacillus simplex and application thereof |
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| CN101671641B (en) * | 2009-08-12 | 2011-05-11 | 南京农业大学 | Bio-controlling bacterial strain AT31 for preventing and curing greenhouse vegetable root knot nematode disease and rice sheath blight disease |
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| CN102925387A (en) * | 2012-10-26 | 2013-02-13 | 沈阳农业大学 | Bacillus simplex for inducing soybean to generate soybean cyst nematode resistance and application |
| WO2014079766A1 (en) * | 2012-11-22 | 2014-05-30 | Basf Se | Pesticidal mixtures |
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| CN108531430A (en) * | 2018-05-09 | 2018-09-14 | 青海省农林科学院 | A kind of simple bacillus S62 and its application |
| CN113248308A (en) * | 2021-05-20 | 2021-08-13 | 施可丰化工股份有限公司 | Microbial agent for effectively preventing and treating root knot nematode disease and preparation method thereof |
| CN113248308B (en) * | 2021-05-20 | 2022-07-01 | 施可丰化工股份有限公司 | Microbial agent for effectively preventing and treating root knot nematode disease and preparation method thereof |
| CN113785765A (en) * | 2021-09-15 | 2021-12-14 | 中国农业大学 | Kit for visually researching interaction relation between plant and nematode, method and application |
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| CN109769858B (en) | 2020-12-11 |
| CN105695376B (en) | 2019-03-19 |
| CN109769858A (en) | 2019-05-21 |
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