CN106986719A - And anti-root rot and seed coat agent, the preparation method of soybean cyst nematode - Google Patents
And anti-root rot and seed coat agent, the preparation method of soybean cyst nematode Download PDFInfo
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- C—CHEMISTRY; METALLURGY
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- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
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- A—HUMAN NECESSITIES
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- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/14—Boron; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
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- A—HUMAN NECESSITIES
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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Abstract
The invention discloses a kind of and anti-root rot and the seed coat agent of soybean cyst nematode, using bacterial strain fermentation liquor as matrix, 3 5g copper sulphate, 8 10g ammonium molybdate, 1 3g boric acid, 15 18g manganese sulfate, 2 4g ferrous sulfate, 70 80g potassium dihydrogen phosphate, 40 50g potassium sulfate, 30 35g calcium lignosulfonate, 18 23g methylcellulose, 9 13g bonding agent, 15 30mL dyestuffs are added in every liter of bacterial strain fermentation liquor.The seed coat agent can prevent soybean cyst nematode and root rot simultaneously and environment is safe from harm, and can also improve Soil Micro-environment, be had a good application prospect in terms of crop pest management.Present invention also offers the preparation method of the seed coat agent simultaneously.
Description
Technical field
The invention belongs to crop disease control technical field, and in particular to a kind of and anti-root rot and soybean cyst
The seed coat agent of nematodiasis, preparation method.
Background technology
Due to Soybean-planting continuous cropping, to meet stubble again more universal so that it is increasingly serious the problem of soybean diseases and insect pests, wherein
Larger on soybean yields influence is root rot and soybean cyst nematode.Root rot is mainly drawn by sickle-like bacteria etc.
The disease risen, soybean cyst nematode is then to disease caused by soybean root, root rot and soybean born of the same parents as SCN
The serious area of cyst mematode can cause a large amount of underproduction of soybean, or even total crop failure, No kernels or seeds are gathered, as in a year of scarcity.Therefore, in soybean planting process
In, it is necessary to strengthen the preventing and controlling to above two pest and disease damage.
But seed coat agent of the prior art is mostly just for a kind of disease, act on single, lacking both can be while have prevented
Root rot and soybean cyst nematode and the controlling agent being safe from harm to environment.
The content of the invention
A kind of and anti-root rot and the seed coat agent of soybean cyst nematode that the present invention is provided and preparation method thereof, should
Seed coat agent be it is a kind of can be while the controlling agent for preventing root rot and soybean cyst nematode and being safe from harm to environment.
The seed coat agent of a kind of provide of the present invention and anti-root rot and soybean cyst nematode, using bacterial strain fermentation liquor as
3-5g copper sulphate, 8-10g ammonium molybdate, 1-3g boric acid, 15-18g sulfuric acid is added in matrix, every liter of bacterial strain fermentation liquor
Manganese, 2-4g ferrous sulfate, 70-80g potassium dihydrogen phosphate, 40-50g potassium sulfate, 30-35g calcium lignosulfonate, 18-
23g methylcellulose, 9-13g bonding agent, 15-30mL dyestuffs；
The bacterial strain fermentation liquor is by bacillus megaterium zymotic fluid, simple fermentation of bacillus liquid, the Chinese root of Fred
Knurl fermented liquid, penicillium chrysogenum fermented liquid are mixed, wherein bacillus megaterium, simple bacillus, Fred China
Rhizobium, the viable count ratio of penicillium chrysogenum are 3:1:1:0.05, wherein the viable count of penicillium chrysogenum is with spore count
Meter.
It is preferred that, viable count is 10 in the bacillus megaterium zymotic fluid8CFU/mL；The simple bacillus hair
Viable count is 10 in zymotic fluid8CFU/mL；Viable count is 10 in the Fred Sinorhizobium fermented liquid8CFU/mL；It is described
Penicillium chrysogenum fermented liquid miospore number is 107Individual/mL
It is preferred that, 4.5g copper sulphate, 9g ammonium molybdate, 2g boric acid, 17.5g sulphur is added in every liter of bacterial strain fermentation liquor
Sour manganese, 2g ferrous sulfate, 75g potassium dihydrogen phosphate, 50g potassium sulfate, 30g calcium lignosulfonate, 20g Methyl celluloses
Element, 11g bonding agent, 25mL dyestuffs.
It is preferred that, the bonding agent is by xanthans and converted starch according to 1:10 mass ratio is mixed.
It is preferred that, the dyestuff is the malachite green solution that concentration is 5-8g/100mL.
Present invention also offers a kind of and anti-root rot and the preparation method of the seed coat agent of soybean cyst nematode, its
It is characterised by, is specifically prepared according to following steps：
Step 1, bacillus megaterium, simple bacillus, Fred Sinorhizobium and penicillium chrysogenum are activated respectively；
Step 2, the bacillus megaterium after activation is seeded in NA fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures
It is 10 to viable count8CFU/mL, obtains bacillus megaterium zymotic fluid；
Simple bacillus after activation is seeded in NA fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures are thin to living
Born of the same parents' number is 108CFU/mL, obtains simple fermentation of bacillus liquid；
Fred Sinorhizobium after activation is seeded in TY fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures are extremely
Viable count is 108CFU/mL, obtains Fred Sinorhizobium fermented liquid；
Penicillium chrysogenum after activation is seeded in PD fluid nutrient mediums, 28 DEG C, 120rmp shaking table cultures to spore count
For 107Individual/mL, obtains penicillium chrysogenum fermented liquid；
Step 3, the work according to bacillus megaterium, simple bacillus, Fred Sinorhizobium, penicillium chrysogenum is thin
Born of the same parents' number ratio is 3:1:1:0.05, by bacillus megaterium zymotic fluid, simple fermentation of bacillus liquid, Fred Sinorhizobium
Zymotic fluid, the mixing of penicillium chrysogenum fermented liquid, obtain bacterial strain fermentation liquor；
Step 4, every liter of seed coat agent is prepared according to following steps：
Step 4.1, following each component is weighed respectively：3-5g copper sulphate, 8-10g ammonium molybdate, 1-3g boric acid, 15-
18g manganese sulfate, 2-4g ferrous sulfate, 70-80g potassium dihydrogen phosphate, 40-50g potassium sulfate, 30-35g sulfomethylated lignin
Sour calcium, 18-23g methylcellulose, 9-13g bonding agent, then grind to form powdery respectively, and are well mixed, and obtain solid-state and mix
Compound；
Step 4.2,1L bacterial strain fermentation liquors are taken, the solid mixt of step 4.1 is added, 15-30mL dyestuffs are added, stirring is mixed
It is even, seed coat agent is obtained after coloring.
Simultaneous anti-root rot and the seed coat agent of soybean cyst nematode that the present invention is provided, can prevent soybean born of the same parents simultaneously
Cyst mematode and root rot and environment is safe from harm；The yield of soybean can be significantly improved；Soil Micro-environment can be improved,
Had a good application prospect in terms of control of crop disease.
Biomaterial preservation information explanation
1st, bacillus megaterium, has been preserved in China Committee for Culture Collection of Microorganisms general on 01 10th, 2013
Logical microorganism center, deposit number is CGMCC 7119, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the micro- life of the Chinese Academy of Sciences
Thing research institute, postcode 100101, Classification And Nomenclature is solution bacillus megaterium Bacillus megaterium.
2nd, simple bacillus, has been preserved in China Committee for Culture Collection of Microorganisms general on October 11st, 2012
Logical microorganism center, deposit number is CGMCC 6650, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the micro- life of the Chinese Academy of Sciences
Thing research institute, postcode 100101, Classification And Nomenclature is simple bacillus simplex.
3rd, Fred Sinorhizobium, was preserved in Chinese microorganism strain preservation conservator on 01 10th, 2013
Meeting common micro-organisms center, deposit number is CGMCC 7120, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the Chinese Academy of Sciences
Institute of microbiology, postcode 100101, Classification And Nomenclature is Fred Sinorhizobium Simorhizobium fredii.
4th, Chrysogenum, has been preserved in China Committee for Culture Collection of Microorganisms general on December 14th, 2010
Logical microorganism center, deposit number is CGMCC 4469, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the micro- life of the Chinese Academy of Sciences
Thing research institute, postcode 100101, Classification And Nomenclature is penicillium chrysogenum Penicillium chrysogenum (bacterial strain and patent
Number be ZL 201010593046.0, patent name for " it is a kind of have induction tomato anti-Meloidogyne incognita Chrysogenum and
Using " in the Chrysogenum addressed be identical bacterial strain).
Embodiment
With reference to specific embodiment, the present invention is described in detail, but should not be construed as the limitation of the present invention.Due to
Strain cell quantity can not be controlled accurately, therefore following each strain cell number ratios etc. are about the description of cell number or spore count,
Meet the order of magnitude each required.
The seed coat agent of a kind of provide of the present invention and anti-root rot and soybean cyst nematode, using bacterial strain fermentation liquor as
4.5g copper sulphate, 9g ammonium molybdate, 2g boric acid, 17.5g manganese sulfate, 2g are added in matrix, every liter of bacterial strain fermentation liquor
Ferrous sulfate, 75g potassium dihydrogen phosphate, 50g potassium sulfate, 30g calcium lignosulfonate, 20g methylcellulose, 9-13g
Bonding agent, 15-30mL dyestuffs；
The bacterial strain fermentation liquor is by bacillus megaterium (Bacillus megaterium) zymotic fluid, simple gemma bar
Bacterium (Bacillus simplex) zymotic fluid, Fred Sinorhizobium (Simorhizobium fredii) zymotic fluid, production are yellow
Penicillium notatum (Penicillium chrysogenum) zymotic fluid is mixed, wherein bacillus megaterium, simple bacillus,
Fred Sinorhizobium, the viable count ratio of penicillium chrysogenum are 3:1:1:0.05, the wherein living cells of penicillium chrysogenum
Number is in terms of spore count；
Viable count is 10 in the bacillus megaterium zymotic fluid8CFU/mL；In the simple fermentation of bacillus liquid
Viable count is 108CFU/mL；Viable count is 10 in the Fred Sinorhizobium fermented liquid8CFU/mL；The production is yellow blue or green
Mold fermentation liquid miospore number is 107Individual/mL；It should be noted that above-mentioned bacterial strains nutrient solution uses blood count law technology；
It is preferred that, the bonding agent is by xanthans and converted starch according to 1:10 mass ratio is mixed；
It is preferred that, the dyestuff is the malachite green solution that concentration is 5-8g/100mL.
Meanwhile, based on same inventive concept, present invention also offers a kind of and anti-root rot and soybean cyst line
The preparation method of the seed coat agent of parasitosis, is specifically prepared according to following steps：
Step 1, bacillus megaterium, simple bacillus, Fred Sinorhizobium and penicillium chrysogenum are activated respectively；
Bacillus megaterium and simple bacillus are activated respectively using NA solid mediums；Activated using YMA culture mediums
Fred Sinorhizobium；Penicillium chrysogenum is activated using PDA culture medium；
Step 2, the bacillus megaterium after activation is seeded in NA fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures
It is 10 to viable count8CFU/mL (2d or so), obtains bacillus megaterium zymotic fluid；
Simple bacillus after activation is seeded in NA fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures are thin to living
Born of the same parents' number is 108CFU/mL (2d or so), obtains simple fermentation of bacillus liquid；
Fred Sinorhizobium after activation is seeded in TY fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures are extremely
Viable count is 108CFU/mL (3d or so), obtains Fred Sinorhizobium fermented liquid；
Penicillium chrysogenum after activation is seeded in PD culture mediums, 28 DEG C, 120rmp shaking table cultures to spore count are
107CFU/mL (4d or so), obtains penicillium chrysogenum fermented liquid；
Step 3, the work according to bacillus megaterium, simple bacillus, Fred Sinorhizobium, penicillium chrysogenum is thin
Born of the same parents' number ratio is 3:1:1:0.05, by bacillus megaterium zymotic fluid, simple fermentation of bacillus liquid, Fred Sinorhizobium
Zymotic fluid, the mixing of penicillium chrysogenum fermented liquid, obtain bacterial strain fermentation liquor；
Step 4, every liter of seed coat agent is prepared according to following steps：
Step 4.1, following each component is weighed respectively：3-5g copper sulphate, 8-10g ammonium molybdate, 1-3g boric acid, 15-
18g manganese sulfate, 2-4g ferrous sulfate, 70-80g potassium dihydrogen phosphate, 40-50g potassium sulfate, 30-35g sulfomethylated lignin
Sour calcium, 18-23g methylcellulose, 9-13g bonding agent, then grind to form powdery respectively, and are well mixed, and obtain solid-state and mix
Compound；
Step 4.2,1L bacterial strain fermentation liquors are taken, the solid mixt of step 4.1 is added, then add 15-30mL dyestuffs, are stirred
Mix, seed coat agent is obtained after coloring.
It should be noted that when needing each bacterial strain of mass propgation, the step 2 also culture of expansion step by step including each bacterial strain is walked
Suddenly, wherein, bacillus megaterium and simple bacillus expand culture, Fred Sinorhizobium using NA fluid nutrient mediums
Bacterium expands culture using TY fluid nutrient mediums, and penicillium chrysogenum expands culture using PD fluid nutrient mediums.
It should be noted that each culture medium that the present invention is addressed is using culture medium prescription commonly used in the art and preparation side
Method is prepared and sterilized, and the formula of each culture medium is provided below：
PDA culture medium：Potato 200g, sucrose 10g, agar 17-20g, distilled water to 1000mL.
PD fluid nutrient mediums：Potato 200g, sucrose 10g, distilled water to 1000mL.
NA solid mediums：Beef extract 3g, peptone 10g, sucrose 10g, agar 17-20g, distilled water to 1000mL, pH
To 7.0.
NA fluid nutrient mediums：Beef extract 3g, peptone 5g, yeast extract 1g, sucrose 10g, distilled water to 1000mL, pH
To 6.8-7.0.
YMA medium：Mannitol 10g, dusty yeast 0.8g, bitter salt 0.2g, potassium dihydrogen phosphate 0.25g, phosphoric acid
Hydrogen dipotassium 0.25g, sodium chloride 0.1g, agar 15g, distilled water to 1000mL, pH to 6.8-7.0.
TY fluid nutrient mediums：Tryptone 5g, dusty yeast 3g, calcium chloride 1.3g, distilled water to 1000mL, pH to 6.8-
7.0。
The application method of the seed coat agent of the present invention is as follows：Seed pelleting is carried out using the seed coat agent before sowing, is incited somebody to action using preceding
Seed coat agent is fully shaken up up and down, and seed and seed coat agent are pressed into 1:70 mass ratio mixing, makes all medicaments uniformly mix in seed
Surface, untill mixing thoroughly, seed pelleting is completed after film forming of drying in the shade, you can sowing.
It is preferred that, simultaneous anti-root rot and the seed coat agent of soybean cyst nematode that the present invention is provided, including following reality
Apply example：
Embodiment 1
The seed coat agent of a kind of and anti-root rot and soybean cyst nematode, using bacterial strain fermentation liquor as matrix, and often
Rise addition 4.5g copper sulphate in bacterial strain fermentation liquor, 9g ammonium molybdate, 2g boric acid, 17.5g manganese sulfate, 2g sulfuric acid Asia
Iron, 75g potassium dihydrogen phosphate, 50g potassium sulfate, 30g calcium lignosulfonate, 20g methylcellulose, 1g xanthans, 10g
Converted starch, 25mL malachite green solutions；Wherein the concentration of malachite green solution Malachite Green is 6g/100mL.
The bacterial strain fermentation liquor is by bacillus megaterium zymotic fluid, simple fermentation of bacillus liquid, the Chinese root of Fred
Knurl fermented liquid, penicillium chrysogenum fermented liquid are mixed, wherein bacillus megaterium, simple bacillus, Fred China
Rhizobium, the viable count ratio of penicillium chrysogenum are 3:1:1:0.05, wherein the viable count of penicillium chrysogenum is with spore count
Meter.
Specifically prepare in accordance with the following methods：
Step 1, bacillus megaterium, simple bacillus, Fred Sinorhizobium and penicillium chrysogenum are activated respectively；
Step 2, the bacillus megaterium after activation is seeded in NA fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures
It is 10 to viable count8CFU/mL, obtains bacillus megaterium zymotic fluid；
Simple bacillus after activation is seeded in NA fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures are thin to living
Born of the same parents' number is 108CFU/mL, obtains simple fermentation of bacillus liquid；
Fred Sinorhizobium after activation is seeded in TY fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures are extremely
Viable count is 108CFU/mL, obtains Fred Sinorhizobium fermented liquid；
Penicillium chrysogenum after activation is seeded in PD culture mediums, 28 DEG C, 120rmp shaking table cultures to spore count are 107
Individual/mL, obtains penicillium chrysogenum fermented liquid；
Step 3, according to bacillus megaterium, simple bacillus, Fred Sinorhizobium, penicillium chrysogenum work
Cell number ratio is 3:1:1:0.05, by bacillus megaterium zymotic fluid, simple fermentation of bacillus liquid, Fred Sinorhizobium
Fermented liquid, the mixing of penicillium chrysogenum fermented liquid, obtain bacterial strain fermentation liquor；
Step 4, every liter of seed coat agent is prepared according to following steps：
Step 4.1, following each component is weighed respectively：4.5g copper sulphate, 9g ammonium molybdate, 2g boric acid, 17.5g sulphur
Sour manganese, 2g ferrous sulfate, 75g potassium dihydrogen phosphate, 50g potassium sulfate, 30g calcium lignosulfonate, 20g Methyl celluloses
Element, 1g xanthans, 10g converted starch, then grind to form powdery respectively, and are well mixed, and obtain solid mixt；
Step 4.2,1L bacterial strain fermentation liquors are taken, the solid mixt of step 4.1 is added, then add 25mL malachite green solutions
Afterwards, stir and evenly mix, seed coat agent is obtained after coloring.
Embodiment 2
The seed coat agent of a kind of and anti-root rot and soybean cyst nematode, using bacterial strain fermentation liquor as matrix, and often
Rise copper sulphate, 8g ammonium molybdate, 1g boric acid, 15g manganese sulfate, 3g ferrous sulfate, the 80g that 3g is added in bacterial strain fermentation liquor
Potassium dihydrogen phosphate, 45g potassium sulfate, 35g calcium lignosulfonate, 23g methylcellulose, 1.1g xanthans, 11g change
Property starch, peacock green；15mL malachite green solutions；Wherein the concentration of malachite green solution Malachite Green is 8g/100mL.
The bacterial strain fermentation liquor is by bacillus megaterium zymotic fluid, simple fermentation of bacillus liquid, the Chinese root of Fred
Knurl fermented liquid, penicillium chrysogenum fermented liquid are mixed, wherein bacillus megaterium, simple bacillus, Fred China
Rhizobium, the viable count ratio of penicillium chrysogenum are 3:1:1:0.05, wherein the viable count of penicillium chrysogenum is with spore count
Meter.
Specifically prepare in accordance with the following methods：
Step 1, bacillus megaterium, simple bacillus, Fred Sinorhizobium and penicillium chrysogenum are activated respectively
Bacterium；
Step 2, the bacillus megaterium after activation is seeded in NA fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures
It is 10 to viable count8CFU/mL, obtains bacillus megaterium zymotic fluid；
Simple bacillus after activation is seeded in NA fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures are thin to living
Born of the same parents' number is 108CFU/mL, obtains simple fermentation of bacillus liquid；
Fred Sinorhizobium after activation is seeded in TY fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures are extremely
Viable count is 108CFU/mL, obtains Fred Sinorhizobium fermented liquid；
Penicillium chrysogenum after activation is seeded in PD culture mediums, 28 DEG C, 120rmp shaking table cultures to spore count are 107
Individual/mL, obtains penicillium chrysogenum fermented liquid；
Step 3, the work according to bacillus megaterium, simple bacillus, Fred Sinorhizobium, penicillium chrysogenum is thin
Born of the same parents' number ratio is 3:1:1:0.05, by bacillus megaterium zymotic fluid, simple fermentation of bacillus liquid, Fred Sinorhizobium
Zymotic fluid, the mixing of penicillium chrysogenum fermented liquid, obtain bacterial strain fermentation liquor；
Step 4, every liter of seed coat agent is prepared according to following steps：
Step 4.1, following each component is weighed respectively：3g copper sulphate, 8g ammonium molybdate, 1g boric acid, 15g sulfuric acid
Manganese, 3g ferrous sulfate, 80g potassium dihydrogen phosphate, 45g potassium sulfate, 35g calcium lignosulfonate, 23g methylcellulose,
1.1g xanthans, 11g converted starch, then grind to form powdery respectively, and are well mixed, and obtain solid mixt；
Step 4.2,1L bacterial strain fermentation liquors are taken, the solid mixt of step 4.1 is added, then add 15mL malachite green solutions
Afterwards, stir and evenly mix, seed coat agent is obtained after coloring.
Embodiment 3
The seed coat agent of a kind of and anti-root rot and soybean cyst nematode, using bacterial strain fermentation liquor as matrix, and often
Rise addition 5g copper sulphate in bacterial strain fermentation liquor, 10g ammonium molybdate, 3g boric acid, 18g manganese sulfate, 4g ferrous sulfate,
70g potassium dihydrogen phosphate, 40g potassium sulfate, 32g calcium lignosulfonate, 18g methylcellulose, 0.9g xanthans, 9g
Converted starch, 30mL malachite green solutions；Wherein the concentration of malachite green solution Malachite Green is 5g/100mL.
The bacterial strain fermentation liquor is by bacillus megaterium zymotic fluid, simple fermentation of bacillus liquid, the Chinese root of Fred
Knurl fermented liquid, penicillium chrysogenum fermented liquid are mixed, wherein bacillus megaterium, simple bacillus, Fred China
Rhizobium, the viable count ratio of penicillium chrysogenum are 3:1:1:0.05, wherein the viable count of penicillium chrysogenum is with spore count
Meter.
Specifically prepare in accordance with the following methods：
Step 1, bacillus megaterium, simple bacillus, Fred Sinorhizobium and penicillium chrysogenum are activated respectively；
Step 2, the bacillus megaterium after activation is seeded in NA fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures
It is 10 to viable count8CFU/mL, obtains bacillus megaterium zymotic fluid；
Simple bacillus after activation is seeded in NA fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures are thin to living
Born of the same parents' number is 108CFU/mL, obtains simple fermentation of bacillus liquid；
Fred Sinorhizobium after activation is seeded in TY fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures are extremely
Viable count is 108CFU/mL, obtains Fred Sinorhizobium fermented liquid；
Penicillium chrysogenum after activation is seeded in PD fluid nutrient mediums, 28 DEG C, 120rmp shaking table cultures to spore count
For 107Individual/mL, obtains penicillium chrysogenum fermented liquid；
Step 3, the work according to bacillus megaterium, simple bacillus, Fred Sinorhizobium, penicillium chrysogenum is thin
Born of the same parents' number ratio is 3:1:1:0.05, by bacillus megaterium zymotic fluid, simple fermentation of bacillus liquid, Fred Sinorhizobium
Zymotic fluid, the mixing of penicillium chrysogenum fermented liquid, obtain bacterial strain fermentation liquor；
Step 4, every liter of seed coat agent is prepared according to following steps：
Step 4.1, following each component is weighed respectively：5g copper sulphate, 10g ammonium molybdate, 3g boric acid, 18g sulfuric acid
Manganese, 4g ferrous sulfate, 70g potassium dihydrogen phosphate, 40g potassium sulfate, 32g calcium lignosulfonate, 18g methylcellulose,
0.9g xanthans, 9g converted starch, then grind to form powdery respectively, and are well mixed, and obtain solid mixt；
Step 4.2,1L bacterial strain fermentation liquors are taken, the solid mixt of step 4.1 is added, then add 25mL malachite green solutions
Afterwards, stir and evenly mix, seed coat agent is obtained after coloring.
The effect to the present invention is further illustrated below.
The present invention has carried out Field information experiment in Kangping and grand celebration two places respectively.The field test results of Kangping in 2015
It has been shown that, the growth of embodiment 1-3 seed coat agent to soybean seedling has facilitation, the suppression to soybean cyst nematode Heterodera glycines in soil
Rate respectively up to 46.83%, 47.32%, 46.99%, to the preventive effect of root rot up to 18.15%, 17.56%,
19.02%, soybean increases production 29.71%, 28.36%, 30.42% respectively；
The result of the test of Kangping shows, the growth of embodiment 1-3 seed coat agent to soybean seedling has facilitation, to soil
The inhibiting rate of soybean cyst nematode Heterodera glycines is respectively up to 31.83%, 30.84%, 32.12% in earth, and the preventive effect to root rot is reachable
17.09%th, 17.42%, 18.22%, soybean increases production 16.45%, 18.36%, 18.02% respectively.
Above-mentioned experiment shows that seed coat agent of the invention effectively prevents and treats root rot and soybean cyst nematode simultaneously, and
Soybean planting yield can be improved.
, but those skilled in the art once know basic creation although preferred embodiments of the present invention have been described
Property concept, then can make other change and modification to these embodiments.So, appended claims are intended to be construed to include
Preferred embodiment and fall into having altered and changing for the scope of the invention.
Obviously, those skilled in the art can carry out the essence of various changes and modification without departing from the present invention to the present invention
God and scope.So, if these modifications and variations of the present invention belong to the scope of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to comprising including these changes and modification.
Claims (6)
1. the seed coat agent of a kind of and anti-root rot and soybean cyst nematode, it is characterised in that using bacterial strain fermentation liquor as base
Added in matter, every liter of bacterial strain fermentation liquor 3-5g copper sulphate, 8-10g ammonium molybdate, 1-3g boric acid, 15-18g manganese sulfate,
2-4g ferrous sulfate, 70-80g potassium dihydrogen phosphate, 40-50g potassium sulfate, 30-35g calcium lignosulfonate, 18-23g
Methylcellulose, 9-13g bonding agent, 15-30mL dyestuffs；
The bacterial strain fermentation liquor is by bacillus megaterium zymotic fluid, simple fermentation of bacillus liquid, Fred Sinorhizobium
Zymotic fluid, penicillium chrysogenum fermented liquid are mixed, wherein bacillus megaterium, simple bacillus, Fred Sinorhizobium
Bacterium, the viable count ratio of penicillium chrysogenum are 3:1:1:0.05, wherein the viable count of penicillium chrysogenum is in terms of spore count.
2. the seed coat agent of according to claim 1 and anti-root rot and soybean cyst nematode, it is characterised in that institute
It is 10 to state viable count in bacillus megaterium zymotic fluid8CFU/mL；Viable count is in the simple fermentation of bacillus liquid
108CFU/mL；Viable count is 10 in the Fred Sinorhizobium fermented liquid8CFU/mL；The penicillium chrysogenum fermented liquid
Miospore number is 107CFU/mL。
3. the seed coat agent of according to claim 1 and anti-root rot and soybean cyst nematode, it is characterised in that every
Rise addition 4.5g copper sulphate in bacterial strain fermentation liquor, 9g ammonium molybdate, 2g boric acid, 17.5g manganese sulfate, 2g sulfuric acid Asia
Iron, 75g potassium dihydrogen phosphate, 50g potassium sulfate, 30g calcium lignosulfonate, 20g methylcellulose, 11g bonding agent,
25mL dyestuffs.
4. the seed coat agent of the simultaneous anti-root rot and soybean cyst nematode according to claim any one of 1-3, it is special
Levy and be, the bonding agent is by xanthans and converted starch according to 1:10 mass ratio is mixed.
5. the seed coat agent of the simultaneous anti-root rot and soybean cyst nematode according to claim any one of 1-3, it is special
Levy and be, the dyestuff is the malachite green solution that concentration is 5-8g/100mL.
6. the preparation method of the seed coat agent of according to claim 1 and anti-root rot and soybean cyst nematode, its
It is characterised by, is specifically prepared according to following steps：
Step 1, bacillus megaterium, simple bacillus, Fred Sinorhizobium and penicillium chrysogenum are activated respectively；
Step 2, the bacillus megaterium after activation is seeded in NA fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures to work
Cell number is 108CFU/mL, obtains bacillus megaterium zymotic fluid；
Simple bacillus after activation is seeded in NA fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures to viable count
For 108CFU/mL, obtains simple fermentation of bacillus liquid；
Fred Sinorhizobium after activation is seeded in TY fluid nutrient mediums, 28 DEG C, 150rmp shaking table cultures are thin to living
Born of the same parents' number is 108CFU/mL, obtains Fred Sinorhizobium fermented liquid；
Penicillium chrysogenum after activation is seeded in PD fluid nutrient mediums, 28 DEG C, 120rmp shaking table cultures to spore count are 107
Individual/mL, obtains penicillium chrysogenum fermented liquid；
Step 3, according to bacillus megaterium, simple bacillus, Fred Sinorhizobium, penicillium chrysogenum viable count
Ratio is 3:1:1:0.05, bacillus megaterium zymotic fluid, simple fermentation of bacillus liquid, Fred Sinorhizobium are fermented
Liquid, the mixing of penicillium chrysogenum fermented liquid, obtain bacterial strain fermentation liquor；
Step 4, every liter of seed coat agent is prepared according to following steps：
Step 4.1, following each component is weighed respectively：3-5g copper sulphate, 8-10g ammonium molybdate, 1-3g boric acid, 15-18g
Manganese sulfate, 2-4g ferrous sulfate, 70-80g potassium dihydrogen phosphate, 40-50g potassium sulfate, 30-35g calcium lignosulfonate,
The bonding agent of 18-23g methylcellulose, 9-13g, then grinds to form powdery respectively, and is well mixed, and obtains solid mixt；
Step 4.2,1L bacterial strain fermentation liquors are taken, the solid mixt of step 4.1 is added, then add 15-30mL dyestuffs, stirring is mixed
It is even, seed coat agent is obtained after coloring.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154114A (en) * | 2010-12-17 | 2011-08-17 | 沈阳农业大学 | Penicillium Chrysogenun inducing meloidogyne incognita chitwood resistance in tomatoes and use thereof |
| CN102925387A (en) * | 2012-10-26 | 2013-02-13 | 沈阳农业大学 | Bacillus simplex for inducing soybean to generate soybean cyst nematode resistance and application |
| CN105494447A (en) * | 2015-12-23 | 2016-04-20 | 沈阳农业大学 | Biological seed coating agent for controlling soybean root disease and preparation method and application thereof |
-
2016
- 2016-10-26 CN CN201610947077.9A patent/CN106986719A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154114A (en) * | 2010-12-17 | 2011-08-17 | 沈阳农业大学 | Penicillium Chrysogenun inducing meloidogyne incognita chitwood resistance in tomatoes and use thereof |
| CN102925387A (en) * | 2012-10-26 | 2013-02-13 | 沈阳农业大学 | Bacillus simplex for inducing soybean to generate soybean cyst nematode resistance and application |
| CN105494447A (en) * | 2015-12-23 | 2016-04-20 | 沈阳农业大学 | Biological seed coating agent for controlling soybean root disease and preparation method and application thereof |
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Application publication date: 20170728 |