CN105441330A - Efficient phosphorus-dissolving growth-promoting bacterium and bio-fertilizer prepared from same as well as application of efficient phosphorus-dissolving growth-promoting bacterium - Google Patents
Efficient phosphorus-dissolving growth-promoting bacterium and bio-fertilizer prepared from same as well as application of efficient phosphorus-dissolving growth-promoting bacterium Download PDFInfo
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Abstract
The invention discloses an efficient phosphorus-dissolving growth-promoting bacterium (Aspergillus niger) A6 screened from limy soil in northern China and provides a microbial agent and a bio-fertilizer preparation which are prepared from the strain A6. The microbial agent is capable of efficiently dissolving powdered rock phosphate, and the dissolving rate after 5 days reaches 80.4% and exceeds that of phosphorus-dissolving fungi strains reported at present. According to the A6 microbial agent, the validity that phosphorus is difficultly dissolved in soil is remarkably improved, and effective phosphorus in soil is increased by 8.13mu g/g-8.95mu g/g; compared with contrast, the phosphorus absorbing quantity of crops is increased by 82.01%; the corn yield is increased by 15.85%. The efficient phosphorus-dissolving growth-promoting bacterium is significant for the saving of phosphorus fertilizer resources, the sustainable yield increase of grains and the protection of farmland ecological environments.
Description
Technical field
The invention belongs to microbiological art, specifically, relate to a kind of efficient phosphorus-dissolution promotion bacteria, bio-feritlizer preparation prepared therefrom and application.
Background technology
Phosphorus element is the required macroelement being only second to nitrogen of plant-growth, because its validity of fixed action of soil is very low, is difficult to meet plant growth needs (Wild, 1988).Research shows, the phosphate fertilizer be manured into soil has more than 90% to be converted into indissoluble phosphorus, and acid soil is converted into tertiary iron phosphate, aluminum phosphate, and calcareous soil is converted into calcium phosphate (HaginandHadas, 1962; NagelschmidtG, etal.1994).For improving crop yield, Phosphorus Fertilizer Rates increases severely, and phosphate rock resource faces exhaustion crisis.It is estimated, whole world Rock Phosphate (72Min BPL) can use 60-90 (Runge-MetzgerA.1995).The arable land of China more than 90% lacks phosphorus, and use phosphate fertilizer to be the major measure solving crop yield, phosphate fertilizer becomes the bottleneck of restriction China agricultural sustainable development day by day.China is that world Fertilizer consumes the first big country, is also that big country consumed by phosphate fertilizer first, annual P fertilizer consumption amount about 1,100 ten thousand tons.But as everyone knows, China's phosphate rock resource lacks, and middle-low grade is in the majority, is only 20 years working life (Tao Junfa, 2009).Therefore, improve phosphate fertilizer utilising efficiency, excavate soil phosphorus Exploitative potential, extend the work-ing life of phosphate rock resource, to China and even countries in the world agricultural sustainable development significant (Mendesetal., 2013; Kanseetal., 2014).
Molten phosphorus microorganism is by producing organic acid (Mendesetal., 2014; Lietal., 2015), H is secreted
+proton (IllmerandSchinner, 1995; Bagyarajetal.2000) indissoluble phosphorus is converted into available phosphorus, relies on simultaneously and produce plant growth-promoting material (HusseinandJoo, 2015), thus play an important role in the biological effectiveness and crop yield of phosphorus element.In the last hundred years, molten phosphorus microbe research is subject to the great attention of scientist always, expects to improve soil indissoluble P availability and phosphate fertilizer utilising efficiency (Zaidietal., 2009 by inoculating molten phosphorus microbial preparation; Merbachetal., 2010).Due to the phosphate solubilization between different types of phosphorus-solubilizing bacteria or different strains and effect of inoculation most diverse, therefore, the screening of efficient phosphorus-dissolution bacterial strain seems particularly important.
At present, molten phosphorus microorganism is as activating soil indissoluble phosphorus, and the raising biological effectiveness of Rock Phosphate (72Min BPL) and the effective measure of crop yield widely use (Mittaletal., 2008; Shietal., 2014).Research display, no matter under or liquid culture condi dull and stereotyped at solid medium, the effect of P-dissolving fungi is higher than phosphorus bacteria fertilizer (Kuceyetal., 1983; Khanetal., 2010).Molten phosphorus microorganism has increase soil available phosphorus (Kuceyetal., 1989; Shietal., 2014; Yinetal., 2015), promote P acquisition and reduce Phosphorus Fertilizer Rates (SinghandReddy, 2011; Nakayanetal., 2013), promote biological effectiveness (KuceyandLeggett, 1989 of Rock Phosphate (72Min BPL); El-Tarabilyetal, 2008; KAURandREDDY, 2015), and promote effect (Chuangetal., 2006 of plant growth and raising crop yield; Mittaletal., 2008; Ghoshetal., 2015).Have been reported and show, molten phosphorus microorganism and bio-feritlizer thereof drop into and improve in crop yield have a high potential at dissolving indissoluble phosphorus, minimizing phosphate fertilizer.Therefore, in molten phosphorus microbe to screen and application potential research process, should focus on selecting existing higher phosphate solubilization, the high efficient strain of crop yield can be improved again.
Farmland based on calcareous soil accounts for 1/2nd of China's cultivated area, has the grain yield of 50%, but, along with the raising year by year of output, cause the excessive use of chemical fertilizer.At present, the Chinese government pays much attention to the efficient problem of Reducing amount of chemical fertilizer applied, and planning requirement accomplishes chemical fertilizer zero growth rate at the year two thousand twenty.The excessive use of phosphate fertilizer causes the eutrophication of rivers and lakes, needs to reduce phosphate fertilizer usage quantity, improve phosphate fertilizer utilization efficiency, reduces the available phosphorus quantity that environment enters water body.Meanwhile, in order to reach the target of phosphate fertilizer zero growth rate, in the urgent need to improvement and the lifting of biotechnology, molten phosphorus microorganism becomes first-selected environmentally friendly measure undoubtedly.But report display, from calcareous soil, especially less from the work report of the high yield calcareous agricultural land soil screening efficient phosphorus-dissolution aspergillus niger of the excessive use of phosphate fertilizer.
Summary of the invention
The object of the invention is the problem lacking phosphorus for China and phosphate fertilizer utilization efficiency is low, yield potential declines, a kind of extensive adaptive efficient phosphorus-dissolution promotion bacteria, microbiobacterial agent prepared therefrom, bio-feritlizer preparation and application thereof are provided.
In order to realize the object of the invention, the present invention screens a strain and has the fungi A6 dissolving more by force the effect of soil indissoluble phosphorus from China From Shijiazhuang City of Hebei Province high-yielding grain fields soil (calcareous soil), the morphological specificity of comprehensive bacterial strain is observed, physiological and biochemical test, molecular biology sequencing result, finally determine that strains A 6 is for aspergillus niger (Aspergillusniger), now be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, address, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number CGMCCNO.11654, preservation date on November 17th, 2015.
The present invention also provides the microbiobacterial agent containing aspergillus niger A6; Also containing additive, binding agent and protective material in described microbial inoculum.
Wherein, described additive is at least one in silicon-dioxide, kaolin, turfy soil, powdered rice hulls, wheat husk powder, straw powder etc.; Described binding agent is wilkinite or water-soluble vitamins polysaccharide etc.; Described protective material is at least one in glycerine, skimmed milk, vegetables oil, sodium alginate, Mierocrystalline cellulose, chitosan etc.
The present invention also provides the complex micro organism fungicide prepared by aspergillus niger A6 or its microbial inoculum.
The present invention also provides the activator for phosphor element of soil prepared by aspergillus niger A6, its microbial inoculum or complex micro organism fungicide.
The present invention also provides the bio-feritlizer preparation prepared by aspergillus niger A6, its microbial inoculum, complex micro organism fungicide or activator for phosphor element of soil.
Described bio-feritlizer preparation be by the microbial inoculum prepared by aspergillus niger A6 or activator for phosphor element of soil after granulation, can separately as base fertilizer, seed manure can be made use together with seed, bio-feritlizer preparation can be mixed and made into at least one in nitrogenous fertilizer, phosphate fertilizer, potash fertilizer or composite fertilizer again.
The present invention also provides the application in activating soil indissoluble phosphorus of aspergillus niger A6, its microbial inoculum, complex micro organism fungicide, activator for phosphor element of soil or bio-feritlizer preparation.
The present invention also provides aspergillus niger A6, its microbial inoculum, complex micro organism fungicide, activator for phosphor element of soil or bio-feritlizer preparation improving the application in the Phosphorus supply capacity of soil to crop (namely improving ground phosphate rock validity).
The present invention further provides aspergillus niger A6, its microbial inoculum, complex micro organism fungicide, activator for phosphor element of soil or bio-feritlizer preparation and improve the application in crop yield.
Efficient phosphorus-dissolution promotion bacteria provided by the invention--aspergillus niger A6, has following Microbiological Characteristics:
1, A6 bacterium colony is on inorganic phosphorus (tricalcium phosphate is phosphorus source) substratum, and growth is very fast, and cultivate 5d diameter for 25 DEG C and reach 2.0-2.5cm, bacterium colony is smooth, and central authorities are slightly protruding, quality velvet shape; Conidium yellow black, to black, radially distributes, and the back side is colourless or slightly light grey (Fig. 1).
2, PDA substratum vertical spread is rapid, and cultivate 7d diameter for 25 DEG C and reach 8.0-8.5cm, originally bacterium colony is white, and become black heavy fleece shape subsequently, the back side is lark slightly.Radially, conidiophore is different in size for conidial head black.
3, bacterium colony grows rapidly on czapek agar medium, and cultivate 7d diameter for 25 DEG C and reach 9.00cm, bacterium colony is smooth, and central authorities are slightly protruding, quality velvet shape; Conidium yellow black is to black; Conidiophore betides matrix, and top capsule is spherical, can educate comprehensively; Conidial fructification is double-deck; Conidium is spherical, and diameter reaches 4 ~ 6 μm, the obvious sharp wart or smooth of wall tool.
The present invention screens the aspergillus niger A6 of efficient phosphorus-dissolution of a strain suitable for limed type, staple crop from northern China calcareous soil, is particularly suitable for the typical soil in the regions such as China North China, northwest.Experiment shows, under solid, liquid nutrient medium condition, can dissolve Ca
3(PO
4)
2, Rock Phosphate (72Min BPL), bone meal.Studied by activating soil indissoluble phosphorus in strains A 6 pairs of calcareous soils, the action effect that promotes plant growth and improve crop yield, for molten phosphorus microorganism transform at soil phosphorus, biological effectiveness, the phosphate fertilizer efficiency utilization of ground phosphate rock and save in phosphate fertilizer resource etc. and provide valuable microorganism resource and technical support.
Microbiobacterial agent of the present invention can high-efficiency dissolution ground phosphate rock, and within 5 days, dissolution rate reaches 80.4%, exceedes the P-dissolving fungi bacterial strain of report at present.A6 microbiobacterial agent significantly strengthens soil indissoluble P availability, and within 10 days ,-20 angel's soil available phosphorus improve 8.13-8.95 μ g/g; Potted plant crop P uptake by plants is than contrast increase by 82.01%; Corn yield improves 15.85%.Microbiobacterial agent of the present invention is for plant growth-promoting successful; and its action effect is not by the suppression of fertilizer application; can use under normal use chemical fertilizer condition, phosphate fertilizer resource, Continued Increasing Grain Yield and the ecotope that protects the fields be saved to China's agriculture production significant.
The present invention is directed to that China's soil phosphorus validity is low, the accumulation of indissoluble phosphorus, the present situation of phosphate fertilizer utilization efficiency continuous decrease, and the present situation of the molten phosphorus microbial resources of high-efficiency broad spectrum is lacked for China, screening and cultivate the new molten phosphorus microorganism strains being extensively suitable for all kinds of MAIN SOILS of China, various crop, provides technical support for what develop efficient phosphorus-dissolution microbiobacterial agent.
Accompanying drawing explanation
Fig. 1 is the bacterium colony that aspergillus niger A6 bacterial strain of the present invention is formed on inorganic phosphorus (tricalcium phosphate is phosphorus source) substratum.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.If do not specialize, the conventional means that technique means used in embodiment is well known to those skilled in the art, is raw materials usedly commercial goods.
The separation of embodiment 1 aspergillus niger A6 and cultivation
From China's From Shijiazhuang City of Hebei Province agricultural land soil (calcareous soil), screen a strain there is the fungi A6 dissolving more by force the effect of soil indissoluble phosphorus, the morphological specificity of comprehensive bacterial strain is observed, molecular biology sequencing result, finally determines that strains A 6 is for aspergillus niger (Aspergillusniger).
(1) aspergillus niger A6 substratum
Fermentation liquor formulation (kg/t fermented liquid): dipotassium hydrogen phosphate 0.1kg, potassium primary phosphate 0.1kg, sodium-chlor 4.3kg, magnesium sulfate 0.1kg, starch 8.6kg, extractum carnis 4.3kg, peptone 4.3kg, yeast powder 4.3kg, sucrose 8.6kg, ammonium sulfate 2.6kg, calcium carbonate 10kg, ferrous sulfate 0.001kg, soya-bean oil 6.8kg, prepare with water.
(2) cultivation of aspergillus niger A6
By aspergillus niger A6 inoculation on beef broth protein culture medium, cultivate 48h for 28 ~ 30 DEG C, then access 1000mL triangular flask, at 30 DEG C, under 220r/min, cultivate 36h; Be linked in 50L seeding tank by 5v/v% inoculum size, at 220r/min, pH7.5, under air flow 0.5vvm condition, after cultivating 2d, then forward in the fermentor tank of 500L, at 200r/min by 10v/v% inoculum size, under pH7.5, air flow 0.7 ~ 1.0vvm condition, cultivate 3d.
After having fermented, with the sterilizing turfy soil of fermented liquid 1 ~ 3 times of volume, kaolin absorption, obtain Dissolve phosphorus aspergillus niger A6 microbial inoculum.
The preparation of embodiment 2 efficient phosphorus-dissolution promotion microbiobacterial agent
1, the cultivation of aspergillus niger A6
(1) composition (kg/t fermented liquid) of substratum
Zulkovsky starch 10kg, soybean cake powder 10kg, Semen Maydis powder 5kg, glucose 1kg, sucrose 10kg, yeast powder 5kg, (NH
4)
2sO
43kg, K
2hPO
40.2kg, NaCl2.5kg, MgSO
47H
2o0.1kg, CaCO
30.5kg, FeSO
40.001kg, boric acid (1%) 1L, pH7.0, prepare with water.
(2) fermentation culture
Aspergillus niger A6 is inoculated on potato sucrose (PDA) substratum, cultivates 3d for 25 ~ 28 DEG C.Aspergillus niger A6 spore is transferred into 1000mL triangular flask, liquid fermentation and culture 36h under 26-28 DEG C, 220r/min.Then press 1v/v% inoculum size access 50L seeding tank, under 200r/min, pH7.0, air flow 0.7vvm condition, after cultivating 36h, then proceed to 500L fermentor tank by 10v/v% inoculum size, at 220r/min, pH7.0, under air flow 0.7 ~ 1.0vvm, cultivate 3d.
2, the preparation of efficient phosphorus-dissolution promotion microbiobacterial agent
After having fermented; in fermented liquid, protective material sodium alginate etc. is added by the amount of fermented liquid mass percent 0.5-1%; then use the carrier adsorption such as sterilizing turfy soil, kaolin of fermented liquid 1 ~ 3 times of volume, obtain molten phosphorus growth-promoting aspergillus niger A6 microbial inoculum, viable count is 5 × 10
8-1 × 10
9cfu/g.
3, the preparation of bio-feritlizer preparation
The binding agent granulations such as appropriate powdered rice hulls, straw powder and wilkinite are added in above microbial inoculum, the microbial inoculum of variable grain particle diameter is formed as base fertilizer according to need of production, for the mechanical planting with corn, wheat, soybean, cotton seeds, or machinery is manured into soil together with the granulated fertilizers such as urea, diammonium phosphate, potash fertilizer, composite fertilizer.
Embodiment 3 aspergillus niger A6 dissolves the effect of indissoluble phosphorus on solid plate
Experimental subjects: molten phosphorus Promoting bacteria--aspergillus niger A6, and with bacillus megaterium (ATCC14581), visit Lay Penicillium notatum (ATCC20851) for contrast.
In room conditions, with bone meal (bonemeal), tertiary iron phosphate FePO
4, secondary calcium phosphate CaHPO
4, tricalcium phosphate Ca
3(PO
4)
2, octocalcium phosphate Ca
8h
2(PO
4)
6, Morocco's ground phosphate rock (MRP), Yelkin TTS (lecithin), aluminum phosphate AlPO
4, calcium monofluorophosphate Ca
5(PO
4)
3f is indissoluble phosphorus, cultivates 5 days with Pikavskaya solid medium, measures the phosphate solubilization of each bacterial strain.
The bacterium colony of phosphorus-solubilizing bacteria and measurement result display (table 1) of molten phosphorus loop diameter, A6 is at bone meal, FePO
4, CaHPO
4, Ca
3(PO
4)
2, Ca
8h
2(PO
4)
6on substratum for phosphorus source, cultivate 5d and occur transparent molten phosphorus circle in periphery of bacterial colonies, maximum with the molten phosphorus circle of bone meal.Only on tricalcium phosphate substratum, there is molten phosphorus circle in control strain ATCC20851 (Penicilliumbilaii), bacillus megaterium ATCC14581 (Bacillusmegateriumvarphosphaticum) has obvious molten phosphorus circle on bone meal and lecithin medium, but the molten phosphorus circle of 2 control strains is less.
Table 1 aspergillus niger A6 is at the colony growth of different phosphate sources substratum upper 5 day and amount of phosphorus dissolved
Note: alphabetical difference shows significant difference (lower same); "-" indicates without molten phosphorus circle, undetermined available phosphorus.
Cultivate available phosphorus measurement result display in the substratum of 5d, A6 is to Ca
3(PO
4)
2meltage is maximum, reaches 5.21mgP/ flat board, is secondly FePO
4(5.10mgP/ is dull and stereotyped), Ca
8h
2(PO
4)
6(4.81mgP/ is dull and stereotyped), available phosphorus accounts for respectively and adds 30.1%, 21.02% and 24.8% of total phosphorus content; Bone meal and CaHPO
4the available phosphorus of release is respectively 2.72mgP/ flat board and 4.43mgP/ flat board, accounts for and adds 31.2% and 24.4% of total phosphorus content.A6 is to CaHPO
4, Ca
3(PO
4)
2, Ca
8h
2(PO
4)
6and FePO
4dissolving stronger.Contrast fungal bacterial strain ATCC20851 only dissolves Ca
3(PO
4)
2, amount of phosphorus dissolved is that 1.46mgP/ is dull and stereotyped, and amount of phosphorus dissolved only has 7.3% of add-on; Contrast bacterial isolates ATCC14581 only dissolves bone meal and Yelkin TTS, and the available phosphorus of bone meal flat board is that 0.37mgP/ is dull and stereotyped, only has 2.8% of add-on.Visible, the molten phosphorus kind of strains A 6 and amount of phosphorus dissolved all far away more than be greater than strains A TCC20851 and ATCC14581.
The effect of indissoluble phosphorus is dissolved in embodiment 4 aspergillus niger A6 liquid medium within
Under liquid culture condi, carry out the phosphate solubilization test of aspergillus niger A6, to visit Lay Penicillium notatum (ATCC20851) and bacillus megaterium (ATCC14581) for control strain.
Wherein, the liquid culture condi of aspergillus niger A6 is with the description of embodiment 1.
1, phosphorus-solubilizing bacteria is to the dissolving power of Phosphorus in Yunnan Province ore
Phosphorite Ore In Anning Phosphorous Deposit, Yunnan Province stone (ARP) belongs to higher-grade Rock Phosphate (72Min BPL), result display (table 2), aspergillus niger A6 shows good dissolving power under liquid culture condi, and the available phosphorus of its process is 586.6 μ gP/ml, accounts for 80.4% of ground phosphate rock content of tatal phosphorus; The available phosphorus of ATCC20851 process is 244.7P μ g/ml, about has the indissoluble phosphorus of 33.6% to be converted into available phosphorus; The available phosphorus of ATCC14581 process is lower, is only 34.7 μ g/ml, accounts for 4.76% of Rock Phosphate (72Min BPL) total phosphorus content, a little more than the contrast not connecing bacterium; Contrast minimum, available phosphorus is only 4.2 μ gP/ml, only has the ground phosphate rock of 0.58% to dissolve.At the end of cultivation, pH significantly declines, and it is 4.22 that the pH of A6 bacterial strain is down to 3.01, ATCC20851, and contrast is 5.16.Illustrate that molten phosphorus microorganism creates acidic substance in culturing process, take part in the dissolving of indissoluble phosphorus.
2, phosphorus-solubilizing bacteria is to the dissolving power of Morocco's Rock Phosphate (72Min BPL)
Under liquid culture condi, phosphate solubilization mensuration is carried out to A6 and ATCC20851 tri-strain P-dissolving fungi.Result shows, each bacterial strain has obvious effect of solubilizing phosphate, significant difference (table 3) between bacterial strain.Morocco's ground phosphate rock is added in liquid nutrient medium, inoculation A6 bacterial strain is respectively 379.5 μ gP/ml and 6.82 μ gP/ml in the available phosphate concentration of 5d and 10d, the ground phosphate rock dissolved at the end of cultivating 10d accounts for 89.91% of add-on, available phosphate concentration reaches 604.5 μ gP/ml, is significantly higher than contrast bacterium ATCC20851 (77.4 μ gP/ml); The available phosphorus that contrast bacterium ATCC20851 dissolves accounts for 11.51% of the full phosphorus amount of ground phosphate rock, and the available phosphorus that the contrast 10d not connecing bacterial strain cultivates is only 2.7 μ gP/ml, accounts for and adds 0.40% of ground phosphate rock phosphorus content.
Table 2 liquid culture lower 5 days melanomyces A6 are to the solute effect (P μ g/ml) of Phosphorite Ore In Anning Phosphorous Deposit, Yunnan Province stone
Note: a, b, c letter difference shows significant difference between process.
The molten phosphorus aspergillus tubigensis A6 of table 3 is to the dissolving power (P μ g/ml) of Morocco Rock Phosphate (72Min BPL) in liquid nutrient medium
| Process | 5 days | pH | 10 days | pH | Available phosphorus (P) | Molten phosphorus % |
| Contrast | 1.35c | 5.16a | 1.35c | 6.68a | 2.7c | 0.40 |
| ATCC 20851 | 50.3b | 4.22b | 27.1b | 6.75a | 77.4b | 11.51 |
| A6 | 379.5a | 3.01c | 225a | 2.95b | 604.5a | 89.91 |
Note: a, b, c letter difference shows significant difference between process.
Embodiment 5 strains A 6 dissolves the action effect of soil indissoluble phosphorus
1, the effect of solubilizing phosphate of strains A 6 pairs of low P soil
Be inoculated into by phosphorus-solubilizing bacteria A6 in low P soil, no matter sterilized soil is also non-sterilized soil, and available phosphorus is all than the remarkable increase (table 4) of contrast.Soil disinfection has remarkably influenced to A6 effect of solubilizing phosphate, and cultivate 10d soil available phosphorus (P) and reach 12.4 μ gP/g soil, sterile soil available phosphorus increases more, increases by 2.78 μ g/g than contrast available phosphorus; When cultivating 20d, soil available phosphorus slightly increases and reaches 12.96 μ g/g, than contrast increase by 3.91 μ g/g; Unsterilised soil available phosphorus increases less, and the soil available phosphorus cultivating 10d and 20d is respectively 13.44 μ g/g and 14.50 μ g/g, increases by 1.49 μ g/g and 3.55 μ g/g respectively than contrast.With regard to soil available phosphorus content, unsterilised soil is higher than sterile soil, and this is mainly owing to the molten phosphorus effect of indigenous microorganism in soil.
Experiment shows, inoculation A6 and the contrast difference do not inoculated remarkable, soil disinfection and unsterilised process significant difference, and the impact of incubation time on A6 dissolving soil phosphorus is not obvious.A6 has the molten phosphorus effect of more significant soil, has shown remarkable effect of solubilizing phosphate during inoculation culture 10d, and the existence of indigenous microorganism does not have influence on the phosphate solubilization of two phosphorus-solubilizing bacteria strains.
Table 4 low-phosphorous soil aspergillus niger A6 inoculation culture 20 days solute effects to soil phosphorus (P μ g/g soil)
Note: a, b letter difference shows significant difference between process.
2, the effect of solubilizing phosphate of strains A 6 pairs of high effective phosphorus soil
The soil inoculation A6 bacterial strain that soil available phosphorus is higher shows stronger molten phosphorus effect (table 5) equally, under the unsterilised condition of soil, the soil available phosphorus that strains A 6 is inoculated reaches 33.20 μ g/g, add 8.13 μ g/g than 25.07 μ g/g of contrast (CK), improve 32.43%; Under soil disinfection condition, the soil available phosphorus of inoculation microbial inoculum A6 reaches 29.67 μ g/g, adds 8.95 μ g/g, improves 43.19% than CK.Unsterilised soil inoculation A6 available phosphorus content is higher than sterile soil 4 ~ 5 μ g/g soil, and sterile soil inoculation A6 available phosphorus increasing amount is higher than unsterilised soil, and available phosphorus absolute value reaches 8.95 μ g/g soil, is greater than 8.13 μ g/g soil of unsterilised soil.The unsterilised lifting being more conducive to available phosphorus of soil is described, sterilizing is conducive to the effect playing phosphorus-solubilizing bacteria strain A6 activating soil indissoluble phosphorus.
The table 5 high phosphorus soil aspergillus niger A6 inoculation culture impact on soil available phosphorus in 15 days (μ g/g soil)
Note: a, b letter difference shows significant difference between process.
Embodiment 6 tricalcium phosphate, ground phosphate rock be under the condition of phosphorus source aspergillus niger A6 on the impact of corn growth
In greenhouse pot culture test, phosphorus-solubilizing bacteria is selected good strains in the field for seed and is used aspergillus niger A6, and take ATCC14581 as contrast, using dosage is 5ml/ basin, and thalline quantity is 3 × 10
8individual/mL.Cultivation matrix is vermiculite (500g/ basin), and phosphorus source is Jinning County's Rock Phosphate (72Min BPL) and tricalcium phosphate, and trial crops is corn, and vegetative period is 50 days.
Result shows, aspergillus niger A6 significantly improves Corn Biomass.Under supply tricalcium phosphate condition, the Corn Biomass of aspergillus niger A6 is 1.05g/ basin, than contrast volume increase 34.61%; Under supply ground phosphate rock condition, the Corn Biomass of aspergillus niger A6 is 0.48g/ basin, than contrast volume increase 41.2% (table 6).
The lower tricalcium phosphate of table 6 phosphorus-solubilizing bacteria A6 effect, ground phosphate rock are on the impact (dry weight g/ basin) of corn growth
Note: a, b, c represent significant difference between treatment group, ab represent this treatment group and a, b process group difference not remarkable.
Embodiment 7 Dissolve phosphorus aspergillus niger A6 is on the impact of soil available phosphorus, biomass and P uptake by plants
Experiment process comprises soil, and not inoculate any bacterial strain be control group (CK), experimental group inoculating strain A6, indoor without crop cultivation 30d, then long-term cropping 30d gathers in the crops, measure soil available phosphorus and Corn Biomass and crop P uptake by plants, determine that different strains is to the conversion capability of soil phosphorus and growth-promoting effect.
1, P-dissolving fungi is on the impact of soil available phosphorus
Inoculation bacteria agent is after 30 days, and three kinds of calcareous soils significantly increase than the available phosphorus of contrast, and No. 1 native CK available phosphorus is 10.16 μ g/g, and inoculation A6 has brought up to 10.31 μ g/g, and slightly improve than contrast, difference is not remarkable; Phosphorus-solubilizing bacteria A6 shows the effect increasing available phosphorus on No. 2 soil, and soil available phosphorus content is the highest, reaches 6.50 μ g/g, improves 35.7%, significant difference than contrast (4.79 μ g/g); It is 4.98 μ g/g that phosphorus-solubilizing bacteria A6 increases soil available phosphorus on No. 3 soil, and a little more than corresponding contrast (4.36 μ g/g), but difference is not remarkable.No. 2, No. 3 soil are similarly low P soil, but No. 3 soil inoculation effects are lower than No. 2 soil (table 7).
2, P-dissolving fungi is on the impact of Corn Biomass
The most obvious aftereffect that phosphorus-solubilizing bacteria is used shows the impact on crop biomass, and the biomass 3 kinds of soil being inoculated phosphorus-solubilizing bacteria is all significantly higher than contrast.No. 1 soil, potted plant corn inoculation A6 bacterial strain, biomass is 0.797g/ basin, than contrast raising 33.5%; In No. 2 soil, the biomass of inoculating strain A6 process is 0.787g/ basin, than contrast increase by 13.2%; On No. 3 soil, the Corn Biomass of inoculating strain A6 is 0.812g/ basin, than contrast raising 14.4% (table 7).
3, A6 microbial inoculum is on the impact of corn P uptake by plants
Result shows, and the calcareous soil of three kinds of different available phosphorus contents is inoculated phosphorus-solubilizing bacteria A6 microbial inoculum, significantly improves the P uptake by plants (table 7) of corn.On No. 1 soil that available phosphorus is higher, not inoculating A6 corn P uptake by plants is 0.6567mg/ basin, and the corn P uptake by plants of inoculation A6 microbial inoculum process reaches 1.1955mg/ basin, than contrast increase by 82.01%; On No. 2 soil that available phosphorus is lower, not inoculating A6 corn P uptake by plants is 0.834mg/ basin, and the corn P uptake by plants of inoculation A6 microbial inoculum process reaches 1.0231mg/ basin, than contrast increase by 22.67%; On No. 3 soil that available phosphorus is very low, not inoculating A6 corn P uptake by plants is 0.994mg/ basin, and the corn P uptake by plants of inoculation A6 microbial inoculum process reaches 1.1468mg/ basin, than contrast increase by 15.37%.
The impact of table 7 phosphorus-solubilizing bacteria agent soil available phosphorus, Corn Biomass and plant P uptake by plants
Note: a, b letter difference shows significant difference between process.
Under embodiment 8 condition of pot, strains A 6 is on the impact of peanut, Sunflower Receptacle biomass
Available phosphorus NaH
2pO
4result display under supply condition, molten phosphorus microorganism A6 shows effect of increasing production (table 8).Phosphorus-solubilizing bacteria A6 inoculates increase yield significantly on peanut, and biomass reaches 30.2g/ basin, than contrast increase by 12.1%; Strains A 6 is inoculated Sunflower Receptacle and is significantly improved biological yield, and biomass is 27.1g/ basin, than contrast increase by 10.6%.
Indissoluble phosphorus Ca
3(PO
4)
2under supply condition, molten phosphorus microorganism A6 shows the action effect (table 8) significantly increasing biomass.Phosphorus-solubilizing bacteria A6 inoculates biomass on peanut and reaches 33.5g/ basin, than contrast volume increase 13.5%; The upper biomass that strains A 6 inoculates Sunflower Receptacle is 32.8g/ basin, than contrast volume increase 34.4%.
Under ground phosphate rock indissoluble phosphorus supply condition, molten phosphorus microorganism A6 significantly increases the biomass (table 8) of peanut and Sunflower Receptacle.The biomass that phosphorus-solubilizing bacteria A6 inoculates peanut is 32.8g/ basin, increases production 14.6% respectively than contrast; The biomass that strains A 6 inoculates Sunflower Receptacle reaches 30.2g/ basin, than contrast volume increase 29.2%.
Above result shows, at indissoluble phosphorus phosphorus source Ca
3(PO
4)
2with rock phosphate powder (RP) under the condition of phosphorus source, the peanut of inoculation A6, Sunflower Receptacle biomass promote effect and are greater than available phosphorus source SODIUM PHOSPHATE, MONOBASIC (NaH
2pO
4), embody phosphorus-solubilizing bacteria A6 microbial inoculum to indissoluble phosphorus forms effect.
Under table 8 different phosphate sources condition, strains A 6 is on the impact of peanut, Sunflower Receptacle biomass
Note: a, b letter difference shows significant difference between process.
The effect of increasing production of embodiment 9 field corn inoculation A6 bio-feritlizer preparation
Result of study shows: the bio-feritlizer preparation applying preparation in embodiment 2 is compared with ATCC20851 with not using bio-feritlizer preparation (CK), and maize straw output, maize grain yield reach significant difference level (table 9).Apply the bio-feritlizer preparation of strains A 6, compared with CK, maize straw output increases 1937kg/hm
2, reach 10988.9kg/hm
2, than contrast increase by 21.40%; Apply microbial inoculum ATCC20851 and CK significant difference, maize straw output increases 581.4kg/hm
2, than contrast volume increase 6.42%.There is significant difference between different strains, the bio-feritlizer preparation of strains A 6 increases 1355.6kg/hm than the maize straw output of ATCC20851 process
2.
The bio-feritlizer preparation of strains A 6 is obvious to maize grain yield impact effect, and compared with CK, maize grain yield reaches 7169.1kg/hm
2, output increased 980.7kg/hm
2, stimulation ratio reaches 15.85%; Microbial inoculum ATCC20851 grain yield increases 565.7kg/hm than without microbial inoculum contrast
2, stimulation ratio is 9.14%.The bio-feritlizer preparation of strains A 6 increases production 415kgkg/hm than ATCC20851
2, significant difference.
The different phosphorus-solubilizing bacteria A6 of table 9 is on the impact of corn yield
Note: a, b, c letter difference shows significant difference between process.
Extensive adaptive efficient phosphorus-dissolution volume increase aspergillus niger A6 and bio-feritlizer preparation thereof have been carried out many-sided research by the present invention, use molten phosphorus bio-feritlizer microbial inoculum of the present invention, can efficiently activating soil phosphorus element, increase soil available phosphorus level, strengthen the biological effectiveness of ground phosphate rock, improve crop P uptake by plants, crop yield is remarkable, reaches the effect of molten phosphorus volume increase, has good agricultural production application and is worth.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Reference
1Wild,A.(Ed.),1988.Russell’sSoilConditionsandPlantGrowth,11thed.Longman,Harlow(chapter21).
2Hagin,J.,Hadas,A.,1962.Solubilityofcalciumphosphatesincalcareoussoils.Nature(London)193,1211-1213.
3NagelschmidtG,etal.Formationofaptitefromsuperphosphateinthesoil.Nature,1994,154:428-429.
4 Tao Jun methods. the Current Situation & Future-China's phosphate rock resource length of service correctly should locating China's phosphate rock resource is analyzed. phosphate fertilizer and compound fertilizer, 2009,5:6-16.
5Runge-MetzgerA.1995.Closingthecycle:obstaclestoefficientPmanagementforimprovedglobalfoodsecurity.InPhosphorusintheGlobalEnvironment:Transfers,CyclesandManagement,ed.HTiessen,pp.27–42.NewYork:Wiley.
6GilbertodeOliveiraMendes&AndréLuizMoreiradeFreitas&OlintoLipariniPereira&IvoRibeirodaSilva&NikolayBojkovVassilev&MaurícioDutraCosta。MechanismsofphosphatesolubilizationbyfungalisolateswhenexposedtodifferentPsources.AnnMicrobiol.,2013,DOI10.1007/s13213-013-0656-3.
7OmkarShankarraoKanse&MelanieWhitelaw-Weckert&TukaramAngadraoKadam&HemalataJanardhanraoBhosale.Phosphatesolubilizationbystress-tolerantsoilfungusTalaromycesfuniculosusSLS8isolatedfromtheNeemrhizosphere.AnnMicrobiol,2014,DOI10.1007/s13213-014-0839-6.
8Mendes,G.D.O.,Freitas,A.L.M.D.,2014.MechanismsofphosphatesolubilizationbyfungalisolateswhenexposedtodifferentPsources.AnnalsofMicrobiology1,239-249.
9XiaolongLi,LijinLuo,JinshuiYang,BaozhenLiandHongliYuan.MechanismsforSolubilizationofVariousInsolublePhosphatesandActivationofImmobilizedPhosphatesinDifferentSoilsbyanEfficientandSalinity-TolerantAspergillusnigerStrainAn2.ApplBiochemBiotechnol(2015)175:2755–2768.
10IllmerPandSchinnerF.Solubilizationofinorganiccalciumphosphates-solubilizationmechanisms[J].SoilBiol.Biochem.,1995,27:3,257～263.
11BagyarajDJ,KrishnarajPU,KhanujaSPS(2000)Mineralphosphatesolubilization:agronomicimplication,mechanismandmoleculargenetics.ProcIndianNatlSciAcad66:69–82.
12KhalidAbdallahHusseinandJinHoJoo.Isolationandcharacterizationofrhizomicrobialisolatesforphosphatesolubilizationandindoleaceticacidproduction.JKoreanSocApplBiolChem,2015,DOI10.1007/s13765-015-0114-y.
13AlmasZaidi,MohammadSaghirKhan,MuneesAhemad,MohdOves,P.A.Wani.RecentAdvancesinPlantGrowthPromotionbyPhosphate-SolubilizingMicrobesin:MohammadSaghirKhanlAlmasZaidilJavedMusarrat(Editors)MicrobialStrategiesforCropImprovement.2009,pp23-50.
14WolfgangMerbach,AnnetteDeubel,AndreasGransee,SilkeRuppel&Anne-KathrinKlamroth.Phosphorussolubilizationintherhizosphereanditspossibleimportancetodeterminephosphateplantavailabilityinsoil.AreviewwithmainemphasisonGermanresults.ArchivesofAgronomyandSoilScience,2010,56(2):119-138.
15MittalV,SinghO,NayyarH,KaurJ,TewariR(2008)Stimulatoryeffectofphosphate-solubilzingfungalstrain(AspergillusawamoriandPenicilliumcitrinum)ontheyieldofchickpea(CicerarietinumL.Cv.GPF2).SoilBiolBiochem40:718–727.
16Shi,F.C.,Yin,Z.W.,Jiang,H.M.,Fan,B.Q.,2014.Screening,identificationofP-dissolvingfungusP83strainanditseffectsonphosphatesolubilizationandplantgrowthpromotion.ACTAMicrobiologicasinica11,1333-1343.(InChinese).
17ZhongweiYin,FachaoShi,HongmeiJiang,DanielP.Roberts,SanfengChen,andBingquanFan..PhosphatesolubilizationandpromotionofmaizegrowthinacalcareoussoilbyPenicilliumoxalicumP4andAspergillusnigerP85.CanadianJournalofMicrobiology,2015,Can.J.Microbiol.61:1–11(2015).dx.doi.org/10.1139/cjm-2015-0358.
18Kucey,RMN.Phosphate-solubilizingbacteriaandfungiinvariouscultivatedandvirginAlbertasoils.CanadianJournalofSoilScience,1983,63:671-678.
19Khan,M.S.,Zaidi,A.,Ahemad,M.,Oves,M.,andWani,P.A.2010.Plantgrowthpromotionbyphosphatesolubilizingfungi-currentperspective.Arch.Agron.SoilSci.56:73-98.
20KuceyRMNandLeggettME.Increasedyieldsandphosphorousuptakebywestarcanola(BrassicanapusL.)inoculatedwithaphosphate-solubilizingisolateofPenicilliumbilaj.Can.J.SoilSci.,1989,69:425-432.
21HimaniSingh,M.SudhakaraReddy.Effectofinoculationwithphosphatesolubilizingfungusongrowthandnutrientuptakeofwheatandmaizeplantsfertilizedwithrockphosphateinalkalinesoils.EuropeanJournalofSoilBiology,2011(47):30-34.
22Nakayan,P.,Hameed,A.,Singh,S.,Young,L.S.,Hung,M.H.,Young,C.C.,2013.Phosphate-solubilizingsoilyeastMeyerozymaguilliermondiiCC1improvesmaize(ZeamaysL.)productivityandminimizesrequisitechemicalfertilization.PlantSoil373,301-315.
23El-Tarabily,KhaledA.；Nassar,AmrH.；Sivasithamparam,Krishnapillai.Promotionofgrowthofbean(PhaseolusvulgarisL.)inacalcareoussoilbyaphosphate-solubilizing,rhizosphere-competentisolateofMicromonosporaendolithica.Appliedsoilecology.2008June.39(2)p.161-171.
24GurdeepKAURandMondemSudhakaraREDDY.EffectsofPhosphate-SolubilizingBacteria,RockPhosphateandChemicalFertilizersonMaize-WheatCroppingCycleandEconomics.Pedosphere,25(3):428–437,2015.
25ChuangCC,KuoYL,ChenCC.SolubilizationofinorganicphosphatesandplantgrowthpromotionbyAspergillusniger.BiologyandFertilityofSoils,2006,DOI10.1007/s00374-006-0140-3.
26PiyasaGhosh,BalaRathinasabapathi,LenaQ.Ma.Phosphorussolubilizationandplantgrowthenhancementbyarsenic-resistantbacteria.Chemosphere,134(2015)1–6.
Claims (10)
1. aspergillus niger (Aspergillusniger) A6, its preserving number is CGMCCNO.11654.
2. the microbiobacterial agent containing aspergillus niger A6 described in claim 1.
3. microbial inoculum according to claim 2, is characterized in that, also containing additive, binding agent and protective material in described microbial inoculum;
Wherein, described additive is at least one in silicon-dioxide, kaolin, turfy soil, powdered rice hulls, wheat husk powder, straw powder;
Described binding agent is wilkinite or water-soluble vitamins polysaccharide;
Described protective material is at least one in glycerine, skimmed milk, vegetables oil, sodium alginate, Mierocrystalline cellulose, chitosan.
4. the complex micro organism fungicide prepared by microbial inoculum described in aspergillus niger A6 described in claim 1 or claim 2,3.
5. the activator for phosphor element of soil prepared by complex micro organism fungicide described in microbial inoculum described in aspergillus niger A6, claim 2,3 described in claim 1 or claim 4.
6. the bio-feritlizer preparation prepared by activator for phosphor element of soil described in complex micro organism fungicide described in microbial inoculum, claim 4 described in aspergillus niger A6, claim 2,3 described in claim 1 or claim 5.
7. bio-feritlizer preparation according to claim 6, is characterized in that, described bio-feritlizer preparation be using the microbial inoculum prepared by aspergillus niger A6 or activator for phosphor element of soil after granulation as base fertilizer; Or, be mixed with at least one in nitrogenous fertilizer, phosphate fertilizer, potash fertilizer or composite fertilizer and form.
8. the application of bio-feritlizer preparation in activating soil indissoluble phosphorus described in activator for phosphor element of soil described in complex micro organism fungicide, claim 5 described in microbial inoculum, claim 4 described in aspergillus niger A6, claim 2,3 described in claim 1 or claim 6,7.
9. bio-feritlizer preparation described in activator for phosphor element of soil described in complex micro organism fungicide, claim 5 described in microbial inoculum, claim 4 described in aspergillus niger A6, claim 2,3 described in claim 1 or claim 6,7 at raising soil to the application in the Phosphorus supply capacity of crop.
10. bio-feritlizer preparation described in activator for phosphor element of soil described in complex micro organism fungicide, claim 5 described in microbial inoculum, claim 4 described in aspergillus niger A6, claim 2,3 described in claim 1 or claim 6,7 is improving the application in crop yield.
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| CN116675583B (en) * | 2023-06-16 | 2024-03-15 | 施可丰化工股份有限公司 | Green efficient soil phosphate fertilizer synergist and preparation method thereof |
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