CN103436453B - Aspergillus niger C2 and microbial inoculum thereof - Google Patents
Aspergillus niger C2 and microbial inoculum thereof Download PDFInfo
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Abstract
The invention discloses an Aspergillus niger C2 of which the collection number is CGMCC NO.7511 and the collection date is July 5th, 2013. The Aspergillus niger C2 is separated from farmland soil in Hengshui Cicy Hebei Province in China, and has been collected at China General Microbiological Culture Collection Center; and the address is Institute of Microbiology in Chinese Academy of Sciences, Beichenxi Road Yard 3 No.3, Chaoyang District, Beijing City. The invention also discloses a microbial inoculum containing the Aspergillus niger C2. The microbial inoculum disclosed by the invention has obvious growth promotion effect on plants without being inhibited by the application of the fertilizer, can be used under the condition of normally using the fertilizer, and has important meanings for phosphate fertilizer resource saving for Chinese agricultural production, continuous yield increase of grains and protection of ecological environment of farmland.
Description
Technical field
The invention belongs to microorganism field, specifically, relate to a kind of efficient phosphorus-dissolution black-koji mould C2 and microbiobacterial agent thereof.
Background technology
The arable land of China more than 90% lacks phosphorus, and use phosphate fertilizer to be the major measure solving crop yield, phosphate fertilizer becomes the bottleneck of restriction China agricultural sustainable development day by day.China is that world Fertilizer consumes the first big country, is also that big country consumed by phosphate fertilizer first, annual P fertilizer consumption amount about 1,100 ten thousand tons.As everyone knows, China's phosphate rock resource lacks, retained reserve 151.98 hundred million tons, and in 20 years, higher-grade phosphorate rock mining totally (Li Zhining, 2003a, Li Zhining, 2003b), has to rely on and buys external high price Rock Phosphate (72Min BPL) Guarantee Grain Production.Some study display, and China's phosphate fertilizer this season utilization ratio is only 3.22 ~ 18%(Zhou Xiaofen, Ma Minqiang, 1994; Zhang Likang, 1997; Cui Zhengzhong etc., 2001; Zhou Guangye etc., 1991; Li Yun etc., 2002).After phosphate fertilizer is manured into soil, 80 ~ 90% very soon by soil fixing (model third congruence, 1998,2004a), and in calcareous soil, available phosphorus is transformed into the extremely low phosphatic rock of solubleness (lschmidt & Nixon, 1944; Murrmann & Peech, 1968), and in acid soil, form tertiary iron phosphate and aluminum phosphate (Ford, 1933).China is since 20 century 70s use phosphate fertilizer, and soil utilization types significantly (Lin Bao, 2009).The soil phosphorus overwhelming majority exists with indissoluble Forms of Inorganic Phosphorus, and slowly, crop validity is low in release.Along with the fertile consumption of rattle increases, transformation of phosphorus fertilizer is that extremely difficult closing of utilization stores state phosphorus (incoming road etc., 2003) and high stability organophosphorus (Huang Qinghai etc., 2003).Solving soil phosphorus forms with release is the key improving soil phosphorus and phosphate fertilizer utilising efficiency.Therefore, strengthen soil phosphorus validity, improving phosphate fertilizer yield increase effect, is the road of the certainty of China's agricultural sustainable development.
Molten phosphorus microorganism is the beneficial microorganism that a class has activating soil indissoluble inorganic phosphorus ability.Applying molten phosphorus activation of microorganism soil indissoluble phosphorus is universally acknowledged safe, economic and effective biological control measure (Kucey, 1988; Kamble & Mohite, 1996; Morales etc., 2007; El-Gawad etc., 2009), extremely various countries scientist and government pay much attention to.Carry out efficient phosphorus-dissolution microbe research and utilization, for excavate soil phosphorus resource Exploitative potential, improve phosphate fertilizer utilization efficiency, save phosphate fertilizer, the production-increasing function playing the limited phosphate rock resource of China and agricultural sustainable development significant.
P-dissolving fungi (Salih etc., 1989; Wang Yanqiu etc., 1993; Vassileva, 1998; Asea etc., 1988; Abd-Alla & Omar, 2001; Cheng Shuqin etc., 2003; Wakelin etc., 2007; Morales etc., 2007) and phosphorus bacteria fertilizer (Rasal, etc., 2004; Raychaudhuri, etc., 2003; Orhan, etc., 2006; ) all have and significantly increase soil available phosphorus, improve crop yield ability, and save effect (El-Lateef, 1998 of phosphate fertilizer; Suri, etc., 2006).Meanwhile, molten phosphorus microorganism improves phosphate fertilizer utilization efficiency (Tomar etc., 1994; Tomar etc., 1996; Kamble & Mohite, 1996; Model third congruence, 2004a), there are ability (Hamed, 2003 of improving crop quality; El-Gawad, 2009), molten phosphorus microorganism has huge applications potentiality for the biological effectiveness improving soil indissoluble phosphorus and phosphate fertilizer.
It is very wide that research in the past relates to face, for molten phosphorus microbe application provides technical support.But, do not pay attention to for a long time molten phosphorus microorganism on a large scale, large regions and multiple soil, various crop extensive adaptability study, the application soil and crop that a large amount of phosphorus-solubilizing bacteria kind cannot be found to a great extent be more suitable for, be difficult to play the maximum potential dissolving soil phosphorus and strengthen phosphorus amount.The use range and the application potential that seriously limit phosphorus-solubilizing bacteria play.For the molten phosphorus Microbial resources of entirety, may huge waste be caused, cause various countries' molten phosphorus microbiobacterial agent research and production to enter larger mistaken ideas thus.Some countries still produce molten phosphorus microbiobacterial agent with the strain of individual plant phosphorus-solubilizing bacteria, and all soil in the whole country and various on use.Large-scale Rong Lin microbiobacterial agent company in the world, produces microbiobacterial agent JumpStart as Canadian Philom Bios company uses the molten phosphorus of individual plant to visit Lay Penicillium notatum (Penicillium bilaii) (Kucey, 1988,1989).Ruchi Biochemicals company of India uses and produces a kind of molten phosphorus microorganism, is recommended in the whole nation and uses.Belgium produces a strain phosphorus bacteria fertilizer and produces microbial inoculum Phosphorene(Ahmed etc., and 1999).China mainly produces molten phosphorus microbiobacterial agent (Ge Cheng, Wu Wei, 1995 with bacillus megaterium (Bacillus megatarium); Fan Bingquan, 2007).Other countries' situation is roughly similar.Do not consider the best-fit of molten phosphorus microorganism and region, soil type, crop species, the result of use of molten phosphorus microbiobacterial agent is had a strong impact on.
The molten phosphorus microbial resources of wide adaptability (soil, crop) is rare in world wide, and has no the report about eurytopic molten phosphorus microbiobacterial agent.
Summary of the invention
The problem that the present invention lacks phosphorus mainly for China and phosphate fertilizer utilization efficiency is low, yield potential declines, provides the microbiobacterial agent of a kind of extensive adaptive efficient phosphorus-dissolution bacterium and preparation thereof.
The invention provides a kind of black-koji mould (Aspergillus niger) C2, preserving number CGMCC NO.7511, preservation date: on July 5th, 2013.Described black-koji mould C2 is separated from Hebei province of China Hengshui City agricultural land soil, has now been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica.
The present invention also provides a kind of microbiobacterial agent containing black-koji mould C2.
Microbiobacterial agent of the present invention is improving the application in crop yield.
Dissolve phosphorus aspergillus niger bacterium C2 provided by the present invention, it has following Microbiological Characteristics:
C2 bacterium colony is on inorganic phosphorus (tricalcium phosphate is wooden land) substratum, and growth is very fast, and 25 DEG C of 5d diameters reach 2.0-2.5cm, smooth, and central authorities are slightly protruding, quality velvet shape; Conidium structure is a large amount of, and yellow black, to black, presents radial distribution, and the back side is colourless or slightly light grey.;
PDA substratum vertical spread is rapid, 25 DEG C of 7d diameter 8.0-8.5cm, be just white, after become black heavy fleece shape, the back side is lark slightly, and radially, conidiophore is different in size for conidial head black;
Bacterium colony grows rapidly on Czapek's agar, 25 DEG C of 7d diameter 9.00cm, and smooth, central authorities are slightly protruding, quality velvet shape; Conidium structure is a large amount of, and yellow black is to black; Conidiophore betides matrix, and top capsule is spherical, can educate comprehensively; Conidial fructification is double-deck; Conidium is spherical, diameter 4 ~ 6 μm, the obvious sharp wart or smooth of wall tool.
Beneficial effect of the present invention:
Microbiobacterial agent of the present invention is for the growth-promoting successful of plant; and its action effect is not by the suppression of fertilizer application; can use under normal use chemical fertilizer condition, phosphate fertilizer resource, Continued Increasing Grain Yield and the ecotope that protects the fields be saved to China's agriculture production significant.
The present invention is directed to that China's soil phosphorus validity is low, the accumulation of indissoluble phosphorus, the present situation of phosphate fertilizer utilization efficiency continuous decrease, and for the present situation that China lacks high-efficiency broad spectrum molten phosphorus microbial resources, microbiobacterial agent industry still uses the old bacterial classification that subject range is narrow, phosphate solubilization is low, screening and cultivate the new molten phosphorus microorganism strains being extensively suitable for all kinds of MAIN SOILS of China, various crop, provides technical support for what develop efficient phosphorus-dissolution microbiobacterial agent.
The present invention with China's MAIN SOILS for research object, carry out the screening of the molten phosphorus microbial strains of broad-spectrum high efficacy, obtain a large amount of phosphorus-solubilizing bacteria strain, these phosphorus-solubilizing bacteria strains and China's MAIN SOILS is compared through research, the adaptability of staple crop, phosphate solubilization, effect of increasing production, obtain a strain and be widely used in China's In The Soils, the black-koji mould C2 of the efficient phosphorus-dissolution of staple crop, be particularly suitable for south China, the north, northwest, northeast, the typical soil in the regions such as southwest, for molten phosphorus microorganism transforms at soil phosphorus, the aspects such as phosphate fertilizer efficiency utilization and saving phosphate fertilizer resource provide microorganism resource and technical support.
Embodiment
The separation of embodiment 1 black-koji mould C2 and cultivation
(1) black-koji mould C2 substratum
Fermentation liquor formulation (kg/t fermented liquid): dipotassium hydrogen phosphate 0.1, potassium primary phosphate 0.1, sodium-chlor 4.3, magnesium sulfate 0.1, starch 8.6, extractum carnis 4.3, peptone 4.3, yeast powder 4.3, sucrose 8.6, ammonium sulfate 2.6, calcium carbonate 10, ferrous sulfate 0.001, soya-bean oil 6.8, prepares with water.
(2) black-koji mould C2 culturing process
By black-koji mould C2 inoculation on beef broth protein culture medium, cultivate 48h for 28 ~ 30 DEG C, then access 1000mL triangular flask, at 30 DEG C, under 220r/min, cultivate 36h; Be linked in 50L seeding tank by 5% inoculum size, at 220r/min, pH7.5, under air flow 0.5vvm condition, after cultivating 2d, then forward in the fermentor tank of 500L by 10v/v% inoculum size, at 200r/min, pH7.5, under air flow 0.7 ~ 1.0vvm condition, cultivate 3d; After having fermented, with the absorption of the kaolin of 1::3 ~ 6 times, obtain Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum.
The preparation of embodiment 2 efficient phosphorus-dissolution microbiobacterial agent
1. the cultivation of black-koji mould C2
(1) composition (kg/t fermented liquid) of substratum
Zulkovsky starch 10kg, soybean cake powder 10kg, Semen Maydis powder 5kg, glucose 1kg, sucrose 10kg, yeast powder 5kg, (NH
4)
2sO
43kg, K
2hPO
40.2kg, NaCl2.5kg, MgSO
47H
2o0.1kg, CaCO
30.5kg, FeSO
40.001kg, boric acid (1%) 1L, pH7.0, prepare with water.
(2) fermentation culture process
Black-koji mould C2 is inoculated on potato sucrose (PDA) substratum, cultivates 3d for 25 ~ 28 DEG C.Black-koji mould C2 spore is transferred into 1000mL triangular flask, liquid fermentation and culture 36h under 26-28 DEG C, 220r/min.Then by 1% inoculum size access 50L seeding tank, under 200r/min, pH7.0, air flow 0.7vvm condition, after cultivating 36h, then proceed to 500L fermentor tank by 10v/v% inoculum size, at 220r/min, pH7.0, under air flow 0.7 ~ 1.0vvm, cultivate 3d.Add protective material sodium alginate, with the absorption of the silicon-dioxide of 1:3 ~ 6 times.
2. the preparation of efficient phosphorus-dissolution microbiobacterial agent
Respectively Dissolve phosphorus aspergillus niger bacterium C2 is fermented, after fermentation, add protective material sodium alginate.Black-koji mould C2 adsorbs with silicon-dioxide, and viable count is 5 × 10
8-1 × 10
9cfu/g.
3. efficient phosphorus-dissolution microbiobacterial agent granulation
Appropriate powdered rice hulls, straw powder and wilkinite bonded pelleting is added in the thalline adsorbing above, mix, different grain diameter microbial inoculums is formed according to need of production, for the mechanical planting with corn, wheat, soybean, cotton seeds, or machinery is manured into soil together with the granulated fertilizers such as urea, diammonium phosphate, potash fertilizer, composite fertilizer.
Experimental example 3 black-koji mould C2 dissolves the effect of indissoluble phosphorus
1. adopt Dissolve phosphorus aspergillus niger bacterium C2, with bacillus megaterium (ATCC14581), visit Lay Penicillium notatum (ATCC20851) for contrast.In room conditions, take tricalcium phosphate as indissoluble phosphorus, carried out Solid media for plates and cultivated 5 days, determined its phosphate solubilization.
The molten phosphorus circle of black-koji mould C2 and colony diameter ratio reach 3.10, the water-soluble molten (P dissolved in molten phosphorus circle
2o
5) be 15.98mg; The molten phosphorus circle of strains A TCC20851 and ATCC14581 be respectively 1.06 and 1.30 with colony diameter ratio, the amount of phosphorus dissolved (P in molten phosphorus circle
2o
5) be respectively 6.31 and 2.11mg, all lower than bacterial strain black-koji mould C2(table 1).
The strain of table 1 phosphorus-solubilizing bacteria is containing Ca
3(PO
4)
2molten phosphorus circle on substratum and the diameter ratio (d/D) of bacterium colony
Note: a, b, c represent significant difference between treatment group, ab represent this treatment group and a, b process group difference not remarkable.
2., under liquid culture condi, carried out bacterial strain grass black-koji mould C2, visitd the molten phosphorus test of Lay Penicillium notatum (ATCC20851) and bacillus megaterium (ATCC14581).Result shows, and careless black-koji mould C2, ATCC20851 and ATCC14581 dissolving phosphoric acid DFP discharges effective P
2o
5be respectively 818.71,159.7 and 37.2mg/L, molten phosphorus rate is respectively 36.55%, 12.51% and 6.82%.
Black-koji mould C2, ATCC20851 and ATCC14581 dissolved phosphorus breeze discharges effective P
2o
5be respectively 907.8,171.4 and 24.2mg/L, molten phosphorus rate is respectively 20.2%, 3.8% and 0.54%.
Under table 2 liquid shaking bottle concrete conditions in the establishment of a specific crime, (5d) phosphorus-solubilizing bacteria is to tricalcium phosphate, ground phosphate rock solute effect (10.0g/L)
Note: a, b, c represent significant difference between treatment group, ab represent this treatment group and a, b process group difference not remarkable.
The lower tricalcium phosphate of experimental example 4 Dissolve phosphorus aspergillus niger bacterium C2 effect, ground phosphate rock are on the impact of corn growth
In greenhouse pot culture test, phosphorus-solubilizing bacteria is selected good strains in the field for seed and is used black-koji mould C2, and take ATCC14581 as contrast, using dosage is 5ml/ basin, and thalline quantity is 3 × 10
8/ mL.Cultivation matrix is vermiculite (500g//basin), and phosphorus source is Jinning County's Rock Phosphate (72Min BPL) and tricalcium phosphate, and trial crops is corn, and the sowing time is on November 1st, 2012, and vegetative period is 50 days.
Result shows, black-koji mould C2 significantly improves Corn Biomass.Under supply tricalcium phosphate condition, the Corn Biomass of black-koji mould C2 is 1.05g/ basin, than contrast volume increase 34.61%; Under supply ground phosphate rock condition, the Corn Biomass of black-koji mould C2 is 0.48g/ basin, than contrast volume increase 41.2%(table 3).
The lower tricalcium phosphate of table 3 phosphorus-solubilizing bacteria C2 effect, ground phosphate rock are on the impact (dry weight g/ basin) of corn growth
Note: a, b, c represent significant difference between treatment group, ab represent this treatment group and a, b process group difference not remarkable.
Experimental example 5 Dissolve phosphorus aspergillus niger bacterium C2 on different soils to the molten phosphorus growth-promoting effect of corn
Adopt five kinds of soil to be respectively black earth, chernozem, Baijiang soil, moisture soil, cinnamon soil, crop is corn.Test comprises two process: (1) contrast (CK) does not use microbial inoculum; (2) phosphorus-solubilizing bacteria agent process, per kilogram soil uses 5g Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum, and (viable count is 3 × 10
8cfu/g), each process repetition 3 times.Every basin dress soil (unsterilised) 1kg, all process all use urea, SODIUM PHOSPHATE, MONOBASIC, Repone K N P and K chemical fertilizer, and usage quantity is respectively 100mg/kg soil, 120mg/kg soil and 80mg/kg soil (i.e. N-P
2o
5-K
2o:60-55-50mg/kg soil).Chemical fertilizer, phosphorus-solubilizing bacteria agent and mixing with soil are even, sow the corn seed that 5 grain germinations are consistent.Water 250mL/ basin for the first time, keeps the field capacity of 60-70% later, within after emerging one week, impose 0.2% ammonium molybdate 2mL/ basin.Gather in the crops after 60d, measure biomass.
Test-results shows, and under using N P and K chemical fertilizer condition, Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum still shows significant effect of increasing production (table 4).On cinnamon soil, moisture soil, Baijiang soil, black earth and chernozem, corn fresh weight increases by 34.0%, 33.6%, 24.4%, 21.5% and 11.1% respectively; Cinnamon soil, moisture soil, Baijiang soil, black earth, chernozem biomass dry weight increase by 22.2%, 30.0%, 32.6%, 12.8% and 29.9% respectively.
Phosphorus-solubilizing bacteria agent be applicable to moisture soil and corn crop with the use of, effect of increasing production coordinates with corn higher than soil type.The action effect of Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum is not by the suppression of fertilizer application, and Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum can use under normal use chemical fertilizer condition.
Under table 4 different soil, Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum is to the action effect of Corn Biomass
Note: <0.05 represents that between process, the significance of difference reaches 5% standard, and <0.01 represents that between process, the significance of difference reaches 1% standard.
Experimental example 6 Dissolve phosphorus aspergillus niger bacterium C2 on different soils to the molten phosphorus growth-promoting effect of winter wheat
Test soil is moisture soil and cinnamon soil, and crop is wheat; Test comprises two process: (1) contrast (CK) does not use microbial inoculum; (2) phosphorus-solubilizing bacteria agent process, per kilogram soil uses 5g Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum, and (viable count is 3 × 10
8cfu/g), each process repetition 3 times.Every basin fills native 1kg, and all process all use urea, SODIUM PHOSPHATE, MONOBASIC, Repone K chemical fertilizer, and usage quantity is respectively 100mg/kg soil, 120mg/kg soil and 80mg/kg soil (i.e. N-P
2o
5-K
2o:60-55-50mg/kg soil).Chemical fertilizer, phosphorus-solubilizing bacteria agent and mixing with soil are even, sow the wheat seed that 8 grain germinations are consistent.Water 250mL/ basin for the first time, keeps the field capacity of 60-70% later, within after emerging one week, impose 0.2% ammonium molybdate 2mL/ basin.Gather in the crops after 60d, measure biomass.
Test-results shows, and is using under chemical fertilizer condition, and moisture soil and cinnamon soil use Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum can significantly improve the biomass (table 5) of wheat.Moisture soil, cinnamon soil use phosphorus-solubilizing bacteria agent black-koji mould C2, and wheat fresh weight is respectively than contrast 32.1% and 22.9%, and dry weight increases increases by 33.4% and 14.0% respectively, and moisture soil inoculation Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum effect of increasing production is higher than cinnamon soil.Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum can both increase wheat biomass on moisture soil and cinnamon soil, uses chemical fertilizer not affect its production-increasing function effect.Therefore, Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum uses under being adapted at normally using chemical fertilizer condition.
Under table 5 different soil, Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum is to the action effect of wheat biomass
Note: <0.05 represents that between process, the significance of difference reaches 5% standard, and <0.01 represents that between process, the significance of difference reaches 1% standard.
Experimental example 7 Dissolve phosphorus aspergillus niger bacterium C2 on different soils to the molten phosphorus growth-promoting effect of soybean
Test soil is black earth and cinnamon soil, and crop is soybean.Test comprises two process:
(1) contrast (CK) and do not use microbial inoculum;
(2) phosphorus-solubilizing bacteria agent process, per kilogram soil uses 5g phosphorus-solubilizing bacteria agent black-koji mould C2(viable count to be 3 × 10
8cfu/g), each process repetition 3 times.Every basin fills native 1kg, and all process all use urea, SODIUM PHOSPHATE, MONOBASIC, Repone K chemical fertilizer, and usage quantity is respectively 100mg/kg soil, 120mg/kg soil and 80mg/kg soil (i.e. N-P
2o
5-K
2o:60-55-50mg/kg soil).Chemical fertilizer, phosphorus-solubilizing bacteria agent and mixing with soil are even, sow the soybean seeds that 6 grain germinations are consistent.Water 250mL/ basin for the first time, keeps the field capacity of 60-70% later, within after emerging one week, impose 0.2% ammonium molybdate 2mL/ basin.Gather in the crops after 60d.Measure biomass.
Test-results shows, and is using under chemical fertilizer condition, uses phosphorus-solubilizing bacteria agent black-koji mould C2 can both improve the biomass of soybean crops on cinnamon soil, black earth 2 kinds of soil.Black earth uses the soybean biomass dry weight of phosphorus-solubilizing bacteria agent black-koji mould C2 the highest, soybean dry weight is than contrast increase by 52.2%; Cinnamon soil inoculation phosphorus-solubilizing bacteria agent black-koji mould C2 volume increase is less, and soybean dry weight increases to 31.5%.Phosphorus-solubilizing bacteria agent black-koji mould C2 uses on black earth-soybean crops can obtain higher output, uses chemical fertilizer not affect the action effect of phosphorus-solubilizing bacteria agent.
Under table 6 different soil, molten phosphorus microbiobacterial agent is to the action effect of soybean biomass
Note: <0.05 represents that between process, the significance of difference reaches 5% standard, and <0.01 represents that between process, the significance of difference reaches 1% standard.
Experimental example 8 uses the effect of increasing production of black-koji mould C2 under chemical fertilizer condition
Adopt China's 9 kinds of typical soils, 4 kinds of Main Cultivation crops, under soil condition of pot, carry out the molten phosphorus growth-promoting Effect disquisition of molten phosphorus microorganism black-koji mould C2.Test comprises two process: (1) contrast (CK) does not use microbial inoculum; (2) phosphorus-solubilizing bacteria agent process, per kilogram soil uses 5g Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum (3 × 10
8cfu/g), each process repetition 3 times.Every basin fills native 1kg, and all process all use urea, SODIUM PHOSPHATE, MONOBASIC, Repone K N P and K chemical fertilizer, N, P
2o
5, K
2o consumption is respectively 60mg/kg soil, 55mg/kg soil, 50mg/kg soil, grows 40 days results.
Crop biomass fresh weight data presentation, use Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum, China's 9 kinds of MAIN SOILS, 4 kinds of staple crops all show production-increasing function, and biomass amount of increase in production is 11.5-76.6%, on average improves 39.7%.Corn yield increasing 11.5% on corn yield increasing 18.3% and black earth on wheat increase yield 19.7%, cinnamon soil on corn yield increasing 23.8%, moisture soil on soybean yield-increasing 27.7%, Baijiang soil on wheat increase yield 45.5%, black earth on corn yield increasing 47.8%, husky ginger black earth on rape yield increasing 48.8%, moisture soil on rape yield increasing 53.6%, salt moisture soil on corn yield increasing 63.0%, rice soil on corn yield increasing 76.6%, chernozem on grey desert soil.
Crop biomass dry weight data presentation, use Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum, in all 9, soil and 4 kinds of crops can both be increased production, and biomass amount of increase in production is 4.3-91.8%, on average improves 32.7%.Corn yield increasing 4.3% on rape yield increasing 12.7%, cinnamon soil on wheat increase yield 16.4%, salt moisture soil on corn yield increasing 15.6%, moisture soil on corn yield increasing 17.6%, Baijiang soil on wheat increase yield 29.9%, black earth on corn yield increasing 32.2%, husky ginger black earth on corn yield increasing 39.1%, grey desert soil on corn yield increasing 46.1%, moisture soil on soybean yield-increasing 53.5%, chernozem on rape yield increasing 91.8%, black earth on rice soil.
Inoculation Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum, there is best-fit characteristic in soil type and crop, to microbial inoculum reaction performance significant difference.Rape coordinate with rice soil increase production the highest, black earth coordinate with corn increase production best, moisture soil, grey desert soil coordinate with corn increase production higher, husky ginger black earth and wheat increase yield higher.Other soil and crop yield amplitude less.
Table 7 Dissolve phosphorus aspergillus niger bacterium C2 under different soil on the impact (g/ basin) of the biomass of crop
Note: <0.05 represents that between process, the significance of difference reaches 5% standard, and <0.01 represents that between process, the significance of difference reaches 1% standard.
The production-increasing function to corn is used in experimental example 9 Dissolve phosphorus aspergillus niger bacterium C2 microbial inoculum field
Use phosphorus-solubilizing bacteria agent black-koji mould C2 under field test condition, significantly improve Spring Corn Yield.Shijiazhuang cinnamon soil test-results display, compared with not using the contrast of microbial inoculum, uses microbial inoculum black-koji mould C2 every mu to increase corn 81.67kg/ mu, volume increase 15.17%(table 8).
Table 8 phosphorus-solubilizing bacteria strain black-koji mould C2 field uses the effect of increasing production (Shijiazhuang, Cinnamon Soils) to spring maize
Note: <0.01 represents that between process, the significance of difference reaches 1% standard.
Cinnamon soil test-results display under the field condition of Baoding, uses phosphorus-solubilizing bacteria agent black-koji mould C2, significantly improves yield of Summer Corn.Compared with not using the contrast of microbial inoculum, microbial inoculum black-koji mould C2 every mu increases corn 51.9kg/ mu, volume increase 10.2%(table 9).
Table 9 phosphorus-solubilizing bacteria strain black-koji mould C2 field uses the effect of increasing production to summer corn
Note: <0.01 represents that between process, the significance of difference reaches 1% standard.
The present invention by extensive adaptive efficient phosphorus-dissolution volume increase black-koji mould C2 extremely microbial inoculum carried out many-sided research, use phosphorus-solubilizing bacteria agent of the present invention, can efficiency utilization soil phosphorus and improve phosphate fertilizer utilization efficiency, reach the effect of molten phosphorus volume increase simultaneously, there is good agricultural production application and be worth.
Above-described embodiment is only be described the preferred embodiment of the present invention; not scope of the present invention is limited; under not departing from the present invention and designing the prerequisite of spirit; the various distortion that those of ordinary skill in the art make technical scheme of the present invention and improvement, all should fall in protection domain that claims of the present invention determines.
Claims (4)
1. black-koji mould (Aspergillus niger) C2, preserving number CGMCC NO.7511, preservation date: on July 5th, 2013.
2. black-koji mould according to claim 1, is characterized in that: it has following Microbiological Characteristics:
C2 bacterium colony grows comparatively fast on inorganic phosphorus substratum, and 25 DEG C of 5d diameters reach 2.0-2.5cm, smooth, and central authorities are slightly protruding, quality velvet shape; Conidium structure is a large amount of, and yellow black, to black, presents radial distribution, and the back side is colourless or slightly light grey;
PDA substratum vertical spread is rapid, 25 DEG C of 7d diameter 8.0-8.5cm, be just white, after become black heavy fleece shape, the back side is lark slightly, and radially, conidiophore is different in size for conidial head black;
Bacterium colony grows rapidly on Czapek's agar, 25 DEG C of 7d diameter 9.00cm, and smooth, central authorities are slightly protruding, quality velvet shape; Conidium structure is a large amount of, and yellow black is to black; Conidiophore betides matrix, and top capsule is spherical, can educate comprehensively; Conidial fructification is double-deck; Conidium is spherical, diameter 4 ~ 6 μm, the obvious sharp wart or smooth of wall tool.
3. the microbiobacterial agent containing black-koji mould C2 described in claim 1 or 2.
4. microbiobacterial agent according to claim 3 is improving the application in crop yield.
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