CN107603890A - Composite bacteria agent and its preparation technology for alkaline land soil improvement - Google Patents
Composite bacteria agent and its preparation technology for alkaline land soil improvement Download PDFInfo
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- CN107603890A CN107603890A CN201710997853.0A CN201710997853A CN107603890A CN 107603890 A CN107603890 A CN 107603890A CN 201710997853 A CN201710997853 A CN 201710997853A CN 107603890 A CN107603890 A CN 107603890A
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Abstract
The invention discloses a kind of composite bacteria agent for alkaline land soil improvement, include the component of following weight part ratio：Strain：Aspergillus 8~12, fluorescent pseudomonas 18~22, acinetobacter calcoaceticus 18~22, amino acid powder 35~40, bacillus subtilis 18~22, bacillus megaterium 8~12, multiple trichodermin 4~6, ferment bacterium 4~6；Culture medium：Polypeptide biology enzyme 0.8~1.2, molasses 10~12, root-inducing powder 1.5~2.5, glucose 2.5~3.5, peptone 0.3~0.6, also disclose the preparation method of above-mentioned microbial inoculum；Above-mentioned strain has the characteristics of fixed nitrogen, Soluble phosphorus, potassium decomposing, decomposition organic matter, suppression insect pest, the organic conversion of promotion, acceleration corruption organic matter decomposition nutrient, when the Returning Application of Crop Straw on salt-soda soil, add this microbial inoculum, the decomposition rate of field stalk can be accelerated to return, and it is lasting to improve effect to alkaline land soil, the present invention can improved soil, and can accelerate plant growth.
Description
Technical field
The present invention relates to the composite bacteria agent improved for alkaline land soil, a kind of preparation work of composite bacteria agent is further related to
Skill.
Background technology
Saline-alkali soil is the general name of solonchak and alkaline earth.Solonchak refers mainly to chloride or the higher salinization soil of sulfate,
Soil is in alkalescence, but pH value is not necessarily very high.Alkaline earth refers to carbonate containing or the phosphatic soil of weight, and pH value is higher, and soil is in
Alkalescence, the content of organic matter of saline-alkali soil is few, and soil fertility is low, and physicochemical character is poor, and the anions and canons being harmful to crop are more, crop
It is not easy to promote seedling.
China's saline alkali land area is wide, and with agrotechnical continuous development, country increasingly payes attention to the exploitation in salt-soda soil
With utilization.Exploitation to salt-soda soil, mainly salt-soda soil soil property is improved.Traditional saline and alkali land improvement method is：First
Salt-soda soil is poured into substantial amounts of fresh water and carries out the desalinization of soil by flooding or leaching, then soil moved in improve the original, then the soil that had additional nutrients on salt-soda soil, to the saline and alkaline of salt-soda soil
Degree is adjusted, and finally adds substantial amounts of organic fertilizer.Because address, natural environment and climate influence, salt-soda soil accumulation of salt in the surface soil alkali phenomenon is serious,
Make that alkaline land improving effect is poor, and speed is slow, therefore traditional mode of ameliorating effect and unobvious.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of use accelerated organic matter and decompose, prevent the salt affected soil accumulation of salt in the surface soil
In the composite bacteria agent of alkaline land soil improvement.
In order to solve the above technical problems, the technical scheme is that：For the composite bacteria agent of alkaline land soil improvement, bag
Include the component of following weight part ratio：
Strain：Aspergillus 8~12, fluorescent pseudomonas 18~22, acinetobacter calcoaceticus 18~22, amino acid powder 35~40, withered grass
Bacillus 18~22, bacillus megaterium 8~12, multiple trichodermin 4~6, ferment bacterium 4~6；
Culture medium：
Polypeptide biology enzyme 0.8~1.2, molasses 10~12, root-inducing powder 1.5~2.5, glucose 2.5~3.5, peptone 0.3
~0.6.
As preferable technical scheme, include the component of following weight part ratio：
Strain：Aspergillus 9, fluorescent pseudomonas 19, acinetobacter calcoaceticus 19, amino acid powder 36, bacillus subtilis 19, huge bud
Spore bacillus 9, multiple trichodermin 4.5, ferment bacterium 4.5；
Culture medium：
Polypeptide biology enzyme 0.9, molasses 11, root-inducing powder 1.5, glucose 2, peptone 0.3.
As preferable technical scheme, include the component of following weight part ratio：
Strain：It is aspergillus 10, fluorescent pseudomonas 20, acinetobacter calcoaceticus 20, amino acid powder 37, bacillus subtilis 20, huge
Bacillus 10, multiple trichodermin 5, ferment bacterium 5；
Culture medium：
Polypeptide biology enzyme 1, molasses 11, root-inducing powder 2, glucose 3, peptone 0.5.
As preferable technical scheme, include the component of following weight part ratio：
Strain：It is aspergillus 12, fluorescent pseudomonas 22, acinetobacter calcoaceticus 22, amino acid powder 40, bacillus subtilis 22, huge
Bacillus 12, multiple trichodermin 6, ferment bacterium 6；
Culture medium：
Polypeptide biology enzyme 1.2, molasses 12, root-inducing powder 2.5, glucose 3.5, peptone 0.6.
The invention also discloses the preparation method of above-mentioned composite bacteria agent, comprise the following steps：
Step 1: prepare culture medium
The nutrient media components of each weight distribution ratio is taken, sterilizes, mix through oversampling, acid-base value is adjusted to 6.5~7.5,
Form fluid nutrient medium；
Step 2: culture
The strain component of weight is taken, each strain is subjected to the purification and rejuvenation respectively in desinfection chamber, each strain is thrown
Enter in culture medium made from this step, in 25~35 DEG C of temperature environment, shaking table culture 36~72 hours, produce described compound
Microbial inoculum.
As preferable technical scheme, in step 1, the acid-base value is adjusted to 7；
Each strain is put into culture medium made from this step in step 2, in 30 DEG C of temperature environment, shaking table culture
48 hours.
As preferable technical scheme, the fluorescent pseudomonas, the bacillus subtilis, the bacillus megaterium
Distinguish >=10 with the viable count of the acinetobacter calcoaceticus7CFU/g。
By adopting the above-described technical solution, the beneficial effects of the invention are as follows：Above-mentioned strain is vdiverse in function, have fixed nitrogen,
Soluble phosphorus, potassium decomposing, organic matter is decomposed, suppresses insect pest, promote organic conversion, the characteristics of accelerating to degenerate organic matter decomposition nutrient, working as salt
During the Returning Application of Crop Straw of alkali ground, this microbial inoculum is added, can accelerate to return the decomposition rate of field stalk, and change alkaline land soil
Good effect is lasting, the present invention can improved soil, and can accelerates plant growth, substantially increases the improvement efficiency in salt-soda soil, and
It ensure that the improved effect in salt-soda soil.
Embodiment
With reference to embodiment, the present invention is expanded on further.In the following detailed description, only retouched by way of explanation
Some one exemplary embodiments of the present invention are stated.Undoubtedly, one of ordinary skill in the art will recognize, without departing from
In the case of the spirit and scope of the present invention, the described embodiments may be modified in various different ways.Therefore,
Description is inherently illustrative, is not intended to limit the scope of the claims.
Embodiment one：
The composite bacteria agent improved for alkaline land soil, include the component of following weight part ratio：Strain：It is aspergillus 8~12, glimmering
Light pseudomonas 18~22, acinetobacter calcoaceticus 18~22, amino acid powder 35~40, bacillus subtilis 18~22, huge gemma bar
Bacterium 8~12, multiple trichodermin 4~6, ferment bacterium 4~6；Culture medium：Polypeptide biology enzyme 0.8~1.2, molasses 10~12, root-inducing powder
1.5~2.5, glucose 2.5~3.5, peptone 0.3~0.6.
Specifically, the present embodiment includes the component of following weight part ratio：
Strain：Aspergillus 9, fluorescent pseudomonas 19, acinetobacter calcoaceticus 19, amino acid powder 36, bacillus subtilis 19, huge bud
Spore bacillus 9, multiple trichodermin 4.5, ferment bacterium 4.5；
Culture medium：
Polypeptide biology enzyme 0.9, molasses 11, root-inducing powder 1.5, glucose 2, peptone 0.3.
Embodiment two：
The present embodiment is that component addition is different from the difference of embodiment one, and the present embodiment includes following weight part ratio
Component：
Strain：It is aspergillus 10, fluorescent pseudomonas 20, acinetobacter calcoaceticus 20, amino acid powder 37, bacillus subtilis 20, huge
Bacillus 10, multiple trichodermin 5, ferment bacterium 5；
Culture medium：
Polypeptide biology enzyme 1, molasses 11, root-inducing powder 2, glucose 3, peptone 0.5.
Embodiment three：
The present embodiment is that component addition is different from the difference of embodiment one, and the present embodiment includes following weight part ratio
Component：
Strain：It is aspergillus 12, fluorescent pseudomonas 22, acinetobacter calcoaceticus 22, amino acid powder 40, bacillus subtilis 22, huge
Bacillus 12, multiple trichodermin 6, ferment bacterium 6；
Culture medium：
Polypeptide biology enzyme 1.2, molasses 12, root-inducing powder 2.5, glucose 3.5, peptone 0.6.
Although it is different that above three embodiment adds constituent content, preparation method is substantially similar, comprises the following steps：
Step 1: prepare culture medium
The nutrient media components of each weight distribution ratio is taken, sterilizes, mix through oversampling, acid-base value is adjusted to 6.5~7.5,
Form fluid nutrient medium；
Step 2: culture
The strain component of weight is taken, each strain is subjected to the purification and rejuvenation respectively in desinfection chamber, each strain is thrown
Enter in culture medium made from this step, in 25~35 DEG C of temperature environment, shaking table culture 36~72 hours, produce described compound
Microbial inoculum.
Specifically, in step 1, the acid-base value is adjusted to 7；Each strain is put into made from this step in step 2
In culture medium, in 30 DEG C of temperature environment, shaking table culture 48 hours.
Fluorescent pseudomonas described in the present embodiment, the bacillus subtilis, the bacillus megaterium and it is described not
The viable count difference >=10 of lever bacterium7CFU/g, to ensure the activity of strain.
The above-mentioned strain of the present invention is vdiverse in function, has fixed nitrogen, Soluble phosphorus, potassium decomposing, decomposition organic matter, suppression insect pest, promotion
Organic conversion, the characteristics of accelerating to degenerate organic matter decomposition nutrient, when the Returning Application of Crop Straw on salt-soda soil, this microbial inoculum is added,
The decomposition rate of field stalk can be accelerated to return, and it is lasting to improve effect to alkaline land soil, the present invention can improved soil, and can
Accelerate plant growth, substantially increase the improvement efficiency in salt-soda soil, and ensure that the improved effect in salt-soda soil.
General principle, principal character and the advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the simply explanation described in above-described embodiment and specification is originally
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (7)
1. the composite bacteria agent for alkaline land soil improvement, it is characterised in that include the component of following weight part ratio：
Strain：Aspergillus 8~12, fluorescent pseudomonas 18~22, acinetobacter calcoaceticus 18~22, amino acid powder 35~40, withered grass gemma
Bacillus 18~22, bacillus megaterium 8~12, multiple trichodermin 4~6, ferment bacterium 4~6；
Culture medium：
Polypeptide biology enzyme 0.8~1.2, molasses 10~12, root-inducing powder 1.5~2.5, glucose 2.5~3.5, peptone 0.3~
0.6。
2. the composite bacteria agent for alkaline land soil improvement as claimed in claim 1, it is characterised in that including following parts by weight
The component of ratio：
Strain：Aspergillus 9, fluorescent pseudomonas 19, acinetobacter calcoaceticus 19, amino acid powder 36, bacillus subtilis 19, huge gemma bar
Bacterium 9, multiple trichodermin 4.5, ferment bacterium 4.5；
Culture medium：
Polypeptide biology enzyme 0.9, molasses 11, root-inducing powder 1.5, glucose 2, peptone 0.3.
3. the composite bacteria agent for alkaline land soil improvement as claimed in claim 1, it is characterised in that including following parts by weight
The component of ratio：
Strain：Aspergillus 10, fluorescent pseudomonas 20, acinetobacter calcoaceticus 20, amino acid powder 37, bacillus subtilis 20, huge gemma
Bacillus 10, multiple trichodermin 5, ferment bacterium 5；
Culture medium：
Polypeptide biology enzyme 1, molasses 11, root-inducing powder 2, glucose 3, peptone 0.5.
4. the composite bacteria agent for alkaline land soil improvement as claimed in claim 1, it is characterised in that including following parts by weight
The component of ratio：
Strain：Aspergillus 12, fluorescent pseudomonas 22, acinetobacter calcoaceticus 22, amino acid powder 40, bacillus subtilis 22, huge gemma
Bacillus 12, multiple trichodermin 6, ferment bacterium 6；
Culture medium：
Polypeptide biology enzyme 1.2, molasses 12, root-inducing powder 2.5, glucose 3.5, peptone 0.6.
5. the preparation method of the composite bacteria agent for being used for alkaline land soil improvement as described in Claims 1-4 any claim,
It is characterised in that it includes following steps：
Step 1: prepare culture medium
The nutrient media components of each weight distribution ratio is taken, sterilizes, mix through oversampling, acid-base value is adjusted to 6.5~7.5, formed
Fluid nutrient medium；
Step 2: culture
The strain component of weight is taken, each strain is subjected to the purification and rejuvenation respectively in desinfection chamber, each strain is put into this
In culture medium made from step, in 25~35 DEG C of temperature environment, shaking table culture 36~72 hours, the compound bacteria is produced
Agent.
6. the preparation method for the composite bacteria agent improved as claimed in claim 5 for alkaline land soil, it is characterised in that step
In one, the acid-base value is adjusted to 7；
Each strain is put into culture medium made from this step in step 2, in 30 DEG C of temperature environment, shaking table culture 48 is small
When.
7. the preparation method for the composite bacteria agent improved as claimed in claim 5 for alkaline land soil, it is characterised in that described
Fluorescent pseudomonas, the bacillus subtilis, the bacillus megaterium and the acinetobacter calcoaceticus viable count difference >=
107CFU/g。
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| CN201710997853.0A CN107603890A (en) | 2017-10-24 | 2017-10-24 | Composite bacteria agent and its preparation technology for alkaline land soil improvement |
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| CN201710997853.0A CN107603890A (en) | 2017-10-24 | 2017-10-24 | Composite bacteria agent and its preparation technology for alkaline land soil improvement |
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ID=61079430
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108410776A (en) * | 2018-05-07 | 2018-08-17 | 江南大学 | Soil improvement and plantation pure plant source soluble E M microbial inoculums and the culture medium for spreading cultivation |
| CN110981635A (en) * | 2019-12-31 | 2020-04-10 | 武汉楚强生物科技有限公司 | Agricultural microbial agent with nitrogen fixation, phosphorus dissolution, potassium dissolution and cellulose dissolution functions and preparation method thereof |
| CN111575009A (en) * | 2020-05-12 | 2020-08-25 | 山东胜伟盐碱地科技有限公司 | Composite microbial inoculum for improving saline-alkali soil and preparation method thereof |
| CN116333947A (en) * | 2023-05-23 | 2023-06-27 | 山东海岱绿洲生物工程有限公司 | Composite biological agent and preparation method thereof |
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| WO2001032587A1 (en) * | 1999-11-01 | 2001-05-10 | 'piksa Inter' Ltd | Biological addition to organic-mineral fertilizers |
| CN102559549A (en) * | 2011-12-27 | 2012-07-11 | 兰州大学 | Arid desert halophilic-basiphilic microbial agent and preparation method and application thereof |
| CN105132337A (en) * | 2015-10-13 | 2015-12-09 | 刘大鹏 | Complex microbial inoculum |
| CN106520131A (en) * | 2016-08-31 | 2017-03-22 | 山东胜伟园林科技有限公司 | Saline alkali soil improver and preparation method thereof |
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2017
- 2017-10-24 CN CN201710997853.0A patent/CN107603890A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001032587A1 (en) * | 1999-11-01 | 2001-05-10 | 'piksa Inter' Ltd | Biological addition to organic-mineral fertilizers |
| CN102559549A (en) * | 2011-12-27 | 2012-07-11 | 兰州大学 | Arid desert halophilic-basiphilic microbial agent and preparation method and application thereof |
| CN105132337A (en) * | 2015-10-13 | 2015-12-09 | 刘大鹏 | Complex microbial inoculum |
| CN106520131A (en) * | 2016-08-31 | 2017-03-22 | 山东胜伟园林科技有限公司 | Saline alkali soil improver and preparation method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108410776A (en) * | 2018-05-07 | 2018-08-17 | 江南大学 | Soil improvement and plantation pure plant source soluble E M microbial inoculums and the culture medium for spreading cultivation |
| CN110981635A (en) * | 2019-12-31 | 2020-04-10 | 武汉楚强生物科技有限公司 | Agricultural microbial agent with nitrogen fixation, phosphorus dissolution, potassium dissolution and cellulose dissolution functions and preparation method thereof |
| CN111575009A (en) * | 2020-05-12 | 2020-08-25 | 山东胜伟盐碱地科技有限公司 | Composite microbial inoculum for improving saline-alkali soil and preparation method thereof |
| CN116333947A (en) * | 2023-05-23 | 2023-06-27 | 山东海岱绿洲生物工程有限公司 | Composite biological agent and preparation method thereof |
| CN116333947B (en) * | 2023-05-23 | 2023-08-18 | 山东海岱绿洲生物工程有限公司 | Composite biological agent and preparation method thereof |
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