CN104017750A - Salt-resistance Bacillusmethylotrophicus strain LJ and application thereof - Google Patents
Salt-resistance Bacillusmethylotrophicus strain LJ and application thereof Download PDFInfo
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Abstract
The invention provides a salt-resistance Bacillusmethylotrophicus strain LJ and an application thereof. The salt-resistance Bacillusmethylotrophicus strain LJ is classified to be named as Bacillusmethylotrophicus, and the collection number of the salt-resistance Bacillusmethylotrophicus strain LJ is CGMCCNO.7570. By performing salt tolerance analysis on the salt-resistance Bacillusmethylotrophicus strain LJ, performing a pathogenicity test on crops, and performing a test on the inhibition effect of the strain on pathogenic bacteria, results show that the salt tolerance degree of the strain is 25-30%; the strain can not cause pathogenicity to the crops, has relatively ideal inhibition effects on bacterial diseases, viral diseases and fungous diseases of tobaccos, can be compounded with a great number of elements, is long in retention period and high in effective bacterium survival rate, can be applied to saline-alkali soils, and has the effects of improving the soil, resisting the diseases, increasing the yield and the like. The salt-resistance Bacillusmethylotrophicus strain LJ disclosed by the invention ensures that a microbial preparation has the positive effects of preventing the diseases and promoting the growth of plants in special environments; the microbial inoculum has the effects of salt-resistant growth-promoting bacteria and disease-prevention growth-promoting bacteria, and is suitable for improvement of salt marsh, alkali soil and acid soil.
Description
Technical field
The invention belongs to microbial technology field, relate in particular to a kind of salt tolerance methylotrophy type Bacillus strain LJ and application thereof.
Background technology
The outstanding problem in the crops seedling stage productions such as the soil secondary salinization, fertilizer application excess poor efficiency and the soil-borne disease that cause are serious cultivate because of long-term drip irrigation by arid and semi-arid lands, and agricultural microorganism microbial inoculum produce and application in have that viable count is low, the problem such as function singleness, long-lasting and poor stability, therefore be necessary using the microorganism in particular surroundings and plant mutually as plant salt tolerance, diseases prevention and growth-promoting approach the excavating resource to salt tolerant growth-promoting bacterium in different coenocorrelations and disease prevention growth-promoting bacterium and arrangement, biological characteristics and the research of effect machine system.In the face of day by day serious soil salinization phenomenon, separate resistant gene of salt, build transgenic salt-tolerant wheat plant, developing saltings is the research direction that countries in the world are paid close attention to, Facultative Halophiles is also referred to as facultative halophilic bacterium, can have salt environment and without salt environment in growth and breeding, the tolerance of salinity can be up to 20%, its topmost feature is the facultative salt of having a liking for, the environmental field that can grow is wide, be the abundant source of the gene of Facultative Halophiles, although it is higher to be grown in some Natrinema altunense sp tolerance of salinitys of extreme environment, under less salt or salt-free condition, really can not grow.Should there is more general biological significance so separate the Facultative Halophiles obtaining under conventional environment.
Water-soluble salt resistant function bacterium is applicable to the improvement of salt marsh, alkaline earth and acid soil.Water-soluble salt resistant function bacterium can decomposite multiple meta-bolites, by improving the activity of plant materials endoenzyme, promote crop growth, stimulate division and the growth of root system meristematic cell, make seedling root of hair fast, secondary root increases, and the quantity of root increases, root elongation, thereby can greatly strengthen Crop ability.When water-soluble salt resistant function bacterium propagation, can discharge highly active amylase and the multiple promotion factor, improve Fertilizer application rate, promote plant to absorb nutrient; Dissolve unavailable phosphorus in soil, improve fertilizer efficiency; The poly-Vetsin producing has stronger preserve moisture and fertility ability.As Arizona, USA university has collected thousands of kinds of halophytess, find wherein to have several being expected for food crop; By sulfuric acid and the quick sweetening of the soil alkalescence of ferric sulfate.As the U.S., in western part, many areas adopt ammonium sulfate to reduce pH, to improve soil water permeability; On acid soil, plant acidproof perennial grass or liming stone, silicate and acidic phosphorus mine powder etc.
Water-soluble salt resistant function bacterium is applicable to coordinating bio-feritlizer, fertilizer fertilizer, bio-bacterial manure etc. to use together, water-soluble salt resistant function bacterium is more suitable for adding in water soluble fertilizer uses, as sprinkling irrigation, drip irrigation, have to add to and rush fertilising, foliage fertilizer, composite fertilizer, compound organic and inorganic fertilizer, the water-soluble fertilizer of macroelement, the water-soluble fertilizer of moderate-element and trace element water-soluble fertilizer etc.
China saltings is large in number and widely distributed, and transformation is administered and these resources of rational exploitation and utilization are one of important channels of China's agricultural sustainable development, and also to improving the ecological environment, thrust zone economy, society and Ecological Sustainable Development have particularly important meaning.Wherein biological modification has important practical significance, and mainly comprises plantation salt-tolerant plant, uses microbial-bacterial fertilizer etc.
Summary of the invention
The invention provides a kind of salt tolerance methylotrophy type Bacillus strain LJ and application thereof, the bacterial strain of the present invention's higher tolerance of salinity of separation screening from the ecological conditions such as varieties in saline-alkali areas and saltern mud, can be the clone of resistant gene of salt and the structure of transgenic salt-tolerant wheat plant lays the foundation, more can be for the research and development of salt-durable microbe microbial inoculum.
For achieving the above object, the present invention adopts following technical proposals to be achieved:
The present invention screens a kind of salt tolerance methylotrophy type Bacillus strain LJ, its Classification And Nomenclature is methylotrophy type bacillus methylotrophicus, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, its deposit number is: CGMCC NO.7570.
The tolerance of salinity of the resistance to NaCl of described bacterial strain LJ is 25%-30%.Described salt tolerance methylotrophy type Bacillus strain LJ bacterium colony is white in color, surface irregularity, and edge is irregular, and LJ bacterium colony size is 2-4mm, easily provokes, flat, without projection, opaque, thalline rod-short, tool mobility, can form gemma.
The invention provides described salt tolerance methylotrophy type Bacillus strain LJ in the application of preparing in fungistat, described methylotrophy type Bacillus strain LJ has bacteriostatic action to black shank pathogenic bacteria, Alternaria alternate pathogenic bacteria, fusarium tabacinum, tobacco bacterial wilt pathogenic bacteria and tobacco mosaic viruses.
Described methylotrophy type Bacillus strain LJ is to the equal no pathogenicity of wheat, tomato, capsicum and cucumber.
The invention provides described salt tolerance methylotrophy type Bacillus strain LJ in the application of preparing in microbial fertilizer, the water-soluble fertilizer of described bacterial strain LJ and macroelement is composite, more than composite rear viable count reaches 400,000,000/g.
The application of salt tolerance methylotrophy type Bacillus strain LJ described in the present invention also provides in the microbiobacterial agent for the preparation of biological restoration saltings, wherein said methylotrophy type genus bacillus bacteria containing amount is more than 30,000,000,000/g, and microbial inoculum usage quantity is 2kg/ mu.
Compared with prior art, advantage of the present invention and positively effect are: the present invention screens salt tolerance methylotrophy type Bacillus strain from saltings, this bacterial strain is carried out to genetic analysis test and Physiology and biochemistry experiment, determine that this bacterial strain is methylotrophy type genus bacillus; And it is carried out tolerance of salinity analysis, the pathogenic test of farm crop and bacterial strain are tested pathogenic bacterium inhibition, result shows, this bacterial strain tolerance of salinity is 25-30%, described bacterial strain LJ is to farm crop no pathogenicity, and bacterial strain has comparatively ideal inhibition to tobacco bacterial disease, Disease, fungal disease.This bacterial strain can with macroelement carry out composite, long preservative period, effectively viable bacteria rate is high.This bacterial strain can use in saltings, has improvement soil, and opposing disease, improves the good results such as output.The invention solves microbial preparation is that plant disease-proof and growth-promoting etc. play positively effect in particular surroundings, and this microbial inoculum has the effect of salt tolerant growth-promoting bacterium and disease prevention growth-promoting bacterium, is applicable to the improvement of salt marsh, alkaline earth and acid soil.
Brief description of the drawings
Fig. 1 is the flat board cultivation figure of methylotrophy type Bacillus strain LJ of the present invention.
Fig. 2 is the salt tolerance experimental result of methylotrophy type Bacillus strain LJ of the present invention.
Embodiment
Below in conjunction with embodiment, technical scheme of the present invention is described in further detail.
Embodiment 1
One, the separation of salt tolerant bacterial strain and screening
On July 4th, 2012 gathers 24 parts of soil samples from Binzhou, Shandong vega cigarette strain rhizosphere soil, and each soil sampling 1g is suspended in 100ml sterilized water, and is diluted to 10 with this
5doubly.Adopt Gibbons improved culture medium or beef-protein medium to screen, Gibbons improved culture medium formula: casein hydrolyzate 5.0g, Trisodium Citrate 3.0g, yeast soaks powder 10.0g, KCl2.0g, peptone, 5.0g, MgSO
47H
2o, 20g NaCl100g, pH7.0.Utilize 1%, 4%, 7%, 10% NaCl step-sizing salt tolerant bacterial strain.Coat on two kinds of culture medium flat plates with the pipettor earth diluent 0.1ml that fetches earth respectively, cultivate 2～3 days for 30 DEG C, be chosen at the well-grown bacterium colony of 10% salt concn, after the purifying of repeatedly ruling, save backup, screen a strain salt tolerant bacterial strain LJ.
Two, the qualification of salt tolerant bacterial strain LJ
The bacterial strain LJ screening is carried out to flat board and cultivate, visible bacterium colony is white in color, surface irregularity, and edge is irregular, and LJ bacterium colony size is 2-4mm, easily provokes, flat, without projection, opaque, thalline rod-short, tool mobility, can form gemma.The flat board of bacterial strain is cultivated picture as shown in Figure 1.
The salt tolerant bacterial strain LJ screening is carried out to the qualification of bacteria molecule genetic classification: bacterial strain LJ is extracted to DNA, and taking this DNA as template, 16S rDNA universal primer is primer, and 16S rDNA fragment is increased.The fragment of amplification is directly carried out to sequencing.Blast program on the result input NCBI website of order-checking is compared, result shows that the 16s rDNA sequence homology degree of the Bacillus methylotrophicus in bacillus in this bacterial strain 16S rDNA sequence and GenBank gene pool is the highest, and homology reaches 99%.By DNAMAN6.0, the 16S rDNA sequence of existing bacillus in Genbank is carried out to phylogenetic analysis, result shows, bacterial strain LJ16S rDNA and Bacillus methylotrophicus homology are the highest, therefore can this bacterial strain of preliminary judgement the methylotrophy type genus bacillus that is bacillus.
With reference to " common bacteria system identification handbook ", carry out preliminary evaluation by salt tolerant bacterial strain being carried out to Physiology and biochemistry mensuration: Gram-positive, V-P reaction, the clark and Lubsreaction positive, the indoles positive, can anaerobic growth, energy hydrolyzed starch, hydrogen sulfide feminine gender, can utilize Citrate trianion, liquefy gelatin, nitrate reduction, catalase, the catalase positive, can hydrolyzed casein.
According to above-mentioned qualification result, this bacterial strain LJ is accredited as methylotrophy type bacillus methylotrophicus.
The bacterial strain LJ screening is carried out to culture presevation, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC); Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica; Preservation date: on May 8th, 2013; Deposit number is: Bacillus methylotrophicus is numbered CGMCC No.7570.
Three, the Salt Tolerance Analysis of methylotrophy type Bacillus strain LJ
By the salt tolerant bacterial strain LJ bacteria suspension of separation and purification, the same amount of transferring is respectively concentrated in the liquid nutrient medium of different salt concn, 37 DEG C, 200r/min shaking table is cultivated, observe growing state, survey at set intervals its bacteria concentration (OD600), to determine bacterium salt tolerant degree.
Salt tolerance experimental result as shown in Figure 2, visible bacterial strain LJ has wider subject range to sodium-chlor, bacterial strain is not autolyze phenomenon in salt-free situation, reach and in 30% situation, still can keep higher cell concentration in salt concn, under the salt concn of 0-35%, all can grow, the suitableeest salt concn is 25%-30%.
Four, methylotrophy type Bacillus strain LJ is to the pathogenic test experiments of farm crop
With asepsis injector, the suspension of salt tolerant bacterial strain LJ is expelled to the seedling basal part of stem of wheat, tomato, capsicum and cucumber, every seedling 0.5ml, repeats 12 times, and moisturizing 24h starts to observe seedling basal part of stem and whether occurs scab after 3 days.
Test-results shows, Facultative Halophiles suspension inoculation, to wheat, tobacco, corn and watermelon seedlings basal part of stem, is cultivated to 3 days afterwards all without obviously scab appearance.Therefore, can confirm this strain Facultative Halophiles to above-mentioned 4 kinds of crops without potential pathogenic.
Five, the restraining effect of methylotrophy type Bacillus strain LJ to fungoid, bacterial disease, Disease
Adopt dull and stereotyped face-off method to test the bacteriostatic activity of described bacterial strain LJ to indicator, the dull and stereotyped central authorities of 9cm PDA inoculation indicator black shank pathogenic bacteria, inoculating single bacterium colony to be tested apart from indicator bacterium piece 2.8cm place, opposite side does not connect bacterium, for measuring blank indicator radius, 25 DEG C of constant temperature culture 5d, observe indicator mycelial growth situation, measure antibacterial result.
Repeat above-mentioned steps, bacterial strain LJ is used for suppressing to the experiment of tobacco alternaric bacteria (red-star like disease pathogenic bacteria), black shank pathogenic bacteria, sharp Fusariumsp oronoco.Experimental result is as shown in table 1, and this salt tolerant bacterial strain LJ all can reach more than 60% the inhibiting rate of disease.
Adopt double-layer plate method, carry out the antagonistic action experiment of bacterial strain LJ to tobacco bacterial wilt.First in culture dish, pour bottom solid medium into, after solidifying, pour again the semisolid medium of the fermented liquid that contains Stalk Rot and certain extent of dilution bacterial strain to be detected into.Cultivate after for some time, observe transparent circle size, measure antibacterial result, this bacterial strain has stronger restraining effect to bacterial wilt.
Adopt local lesion's method, the TMV tobacco tender leaf of fully morbidity is ground to form to homogenate with sterilizing mortar, with phosphoric acid buffer (PH7.0) the 1:40 dilution proportion after sterilizing.The mixed liquid of Zuo Banye inoculation NB substratum and viral equal-volume compares, the mixed liquid of right half leaf inoculating active strain fermentation supernatant liquor and viral equal-volume deals with, after inoculation, water sprays blade face immediately, after 3d, add up withered spot number order, inhibiting rate method of calculation are as follows: inhibiting rate (%)=[(contrast withered spot number-processing withered spot number)/contrast withered spot number] × 100%.Bacterial strain has reached 72% to the inhibition of TMV, and this bacterial strain is also very outstanding to the inhibition of other diseases, can be used as broad spectrum type disease resistance biocontrol strain.
The inhibition experimental result of table 1 bacterial strain LJ to various disease pathogenic bacterium
Six, methylotrophy type Bacillus strain LJ and macroelement water-soluble fertilizer is composite
Preparation LJ bacterial strain seed liquor, is then inoculated in fermention medium by conventional inoculative proportion, by adjusting fermention medium carbon nitrogen source, pH value, leavening temperature, inoculative proportion optimization mixed fermentation condition, makes the total number of bacterial colony of fermented liquid reach 8 × 10
9cfu/ml.
Mix with certain proportion with carrier, spraying is dry, forms bacterium powder.Bacterium powder living bacteria count is 30,000,000,000/g.With urea, ammonium sulfate, saltpetre, potassium primary phosphate, prepare n-p-k ratio and classify 10-3-15 as, and containing middle micronutrient boron, manganese, iron, magnesium, copper, zinc etc., living bacteria count >=400,000,000/g high-efficiency composite biological macroelement water-soluble fertilizer.This product is at room temperature placed, at set intervals preparation is carried out to plate count, detect the stability of viable count.
Active degradation rule under table 2LJ bacteria preparation room temperature
| Time | 0d | 30d | 60d | 90d | 120d | 180d | 240d | 360d |
| Effective bacterium number (hundred million/g) | 5.26 | 5.24 | 5.01 | 5.03 | 4.96 | 4.52 | 4.39 | 4.25 |
Find out by table 2, this product is at room temperature preserved, along with the prolongation of time, bacterium number slightly declines, and preserves 12 months, and bacterium number is reduced to 4.25 hundred million/g by 5.26 original hundred million/g, illustrate contain salt tolerant active bacterial strain macroelement water-soluble fertilizer at room temperature, effectively the viable bacteria shelf time is long, not perishable.Analyzing its reason is mainly that this bacterial strain contains resistant gene of salt, still can long-term surviving under the environment of high salt; In addition, this bacterial strain is genus bacillus, forms gemma and is easy to storage and transport, has the potential quality that is developed as biological prevention and control agent.
Use the microbial fertilizer or the bio-feritlizer that have added described bacterial strain LJ, the quantity that can obviously reduce saltiness, increase organic content and the Soil Microorganism monoid of salt-affected soil, plays good action for improvement soil physics, chemistry and biological property., using after the microbial fertilizer of different sorts and concentration, the upgrowth situation of plant is all improved meanwhile, and growth result is all better than blank group, and concentration and effect relation remarkable.The active bacterium that bacterial strain LJ contains abundant and high value, has phosphorus decomposing, releases potassium, promotes decomposition of soil organic matter, improves Soil Microorganism group, improves the function of Soil structure, significantly improves the utilization ratio of fertilizer.Can improve retention capacity of soil simultaneously.
Seven, saltings biological restoration
Experiment is mainly carried out in saltings.Field test adopts randomized block design.Taking tobacco as experimental subjects.Two processing, the processing of LJ microbiobacterial agent are established in experiment: methylotrophy type gemma bacillus agent 30,000,000,000/g+ conventional fertilizer application, and microbial inoculum usage quantity is each 2kg/ mu, transplanting and group's phase spread manuer in holes.Control treatment (CK): conventional fertilizer application.Each processing 5 row, 36 cigarette strains of every row, spacing in the rows 0.5m, line-spacing 1m.Each processing arrange 3 parallel.Other cultivation management measures are consistent with land for growing field crops.Investigation finds that using microbiobacterial agent can improve soil physical properties, soil porosity and hair porosity increase, water permeability improves, can increase the soil organism, increase soil fertility, promote the eubiosis of terrestrial space, suppress the earth's surface accumulation of salt in the surface soil, guarantee the growth of crop normal growth, disease and insect resistance and somatotrophic ability are remarkable, and amount of increase in production reaches more than 30%.
Above embodiment is only in order to technical scheme of the present invention to be described, but not is limited; Although the present invention is had been described in detail with reference to previous embodiment, for the person of ordinary skill of the art, the technical scheme that still can record previous embodiment is modified, or part technical characterictic is wherein equal to replacement; And these amendments or replacement do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (10)
1. a salt tolerance methylotrophy type Bacillus strain LJ, is characterized in that: its Classification And Nomenclature is methylotrophy type genus bacillus
bacillus methylotrophicus, being preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, its deposit number is: CGMCC NO.7570.
2. salt tolerance methylotrophy type Bacillus strain LJ according to claim 1, is characterized in that: the tolerance of salinity of the resistance to NaCl of described bacterial strain LJ is 25%-30%.
3. salt tolerance methylotrophy type Bacillus strain LJ according to claim 1, is characterized in that: bacterium colony is white in color, surface irregularity, and edge is irregular, LJ bacterium colony size is 2-4 mm, easily provokes, flat, without projection, opaque, thalline rod-short, tool mobility, can form gemma.
4. salt tolerance methylotrophy type Bacillus strain LJ according to claim 1 is in the application of preparing in fungistat.
5. salt tolerance methylotrophy type Bacillus strain LJ according to claim 4, in the application of preparing in fungistat, is characterized in that: described methylotrophy type Bacillus strain LJ has bacteriostatic action to black shank pathogenic bacteria, Alternaria alternate pathogenic bacteria, fusarium tabacinum, tobacco bacterial wilt pathogenic bacteria and tobacco mosaic viruses.
6. salt tolerance methylotrophy type Bacillus strain LJ according to claim 4, in the application of preparing in fungistat, is characterized in that: described methylotrophy type Bacillus strain LJ is to the equal no pathogenicity of wheat, tomato, capsicum and cucumber.
7. salt tolerance methylotrophy type Bacillus strain LJ according to claim 1 is in the application of preparing in microbial fertilizer.
8. salt tolerance methylotrophy type Bacillus strain LJ according to claim 7, in the application of preparing in microbial fertilizer, is characterized in that: the water-soluble fertilizer of described bacterial strain LJ and macroelement is composite, more than composite rear viable count 400,000,000/g.
9. the application of salt tolerance methylotrophy type Bacillus strain LJ according to claim 1 in the microbiobacterial agent for the preparation of biological restoration saltings.
10. the application of salt tolerance methylotrophy type Bacillus strain LJ according to claim 9 in the microbiobacterial agent for the preparation of biological restoration saltings, it is characterized in that: described methylotrophy type genus bacillus bacteria containing amount is more than 30,000,000,000/g, and microbial inoculum usage quantity is 2 kg/ mus.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103045515A (en) * | 2012-12-27 | 2013-04-17 | 陕西恒田化工有限公司 | Biological agent of bacillus methylotrophicus as well as preparation method and application thereof |
| CN103361288A (en) * | 2013-06-28 | 2013-10-23 | 安徽工程大学 | Methylotrophic bacillus and application thereof |
| CN103039530B (en) * | 2012-12-27 | 2013-12-11 | 陕西恒田化工有限公司 | Wettable powder of bacillus methylotrophicus and preparation method thereof |
-
2014
- 2014-04-14 CN CN201410148953.2A patent/CN104017750B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103045515A (en) * | 2012-12-27 | 2013-04-17 | 陕西恒田化工有限公司 | Biological agent of bacillus methylotrophicus as well as preparation method and application thereof |
| CN103039530B (en) * | 2012-12-27 | 2013-12-11 | 陕西恒田化工有限公司 | Wettable powder of bacillus methylotrophicus and preparation method thereof |
| CN103361288A (en) * | 2013-06-28 | 2013-10-23 | 安徽工程大学 | Methylotrophic bacillus and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| MUNUSAMY MADHAIYAN 等: "Bacillus methylotrophicus sp. nov., a methanol-utilizing,plant-growth-promoting bacterium isolated from rice rhizosphere soil", 《INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY》, vol. 60, 31 December 2010 (2010-12-31), pages 2490 - 2495, XP055219471, DOI: doi:10.1099/ijs.0.015487-0 * |
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| CN106857674B (en) * | 2017-02-09 | 2019-02-26 | 河北省科学院生物研究所 | Application of bacillus methylotrophicus BH21, microbial fertilizer and preparation method thereof |
| CN108753666A (en) * | 2018-07-02 | 2018-11-06 | 山东农业大学 | The preparation and application of one plant of salt tolerant Antagonistic Fungi M4-1 and its microbial inoculum |
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| CN110041117A (en) * | 2019-03-06 | 2019-07-23 | 湖南省蔬菜研究所 | A kind of culture medium and preparation method thereof improving capsicum salt tolerance |
| CN109957535A (en) * | 2019-05-08 | 2019-07-02 | 河北省农林科学院遗传生理研究所（河北省农林科学院农产品质量安全研究中心） | Simple bacillus, microbial bacterial agent, bio-fertilizer and the application prepared using it |
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| CN113293112A (en) * | 2021-06-21 | 2021-08-24 | 上海交通大学 | Composite microbial soil remediation agent and preparation method thereof |
| CN113637612A (en) * | 2021-09-03 | 2021-11-12 | 山东农业大学 | Salt-tolerant strain BY2G20 and application thereof |
| CN117694196A (en) * | 2024-01-19 | 2024-03-15 | 云南省烟草公司丽江市公司 | Comprehensive control method for tobacco virus diseases |
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