CN103361288A - Methylotrophic bacillus and application thereof - Google Patents
Methylotrophic bacillus and application thereof Download PDFInfo
- Publication number
- CN103361288A CN103361288A CN2013102659377A CN201310265937A CN103361288A CN 103361288 A CN103361288 A CN 103361288A CN 2013102659377 A CN2013102659377 A CN 2013102659377A CN 201310265937 A CN201310265937 A CN 201310265937A CN 103361288 A CN103361288 A CN 103361288A
- Authority
- CN
- China
- Prior art keywords
- acetoin
- genus bacillus
- glucose
- methylotrophic bacillus
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides methylotrophic bacillus which can produce acetoin at high yield. The methylotrophic bacillus is collected in the China General Microbiological Culture Collection Center (CGMCC) and has the collection number of CGMCCC No.7485. When the methylotrophic bacillus is fermented under a certain conditions, acetoin with the concentration of 15.71g/L can be obtained. The production capacity of most of the reported acetoin producing bacteria is lower than or equivalent to that of the methylotrophic bacillus CGMCCC No.7485; however, according to the strain, except the glucose concentration is preliminarily optimized, other culture conditions and medium components are not subjected to breeding transformation. Thus, after the methylotrophic bacillus is subjected to further breeding transformation and condition optimization, the acetoin yield is further improved. Therefore, the methylotrophic bacillus has wide application prospects.
Description
Technical field
The invention belongs to microorganism and fermentation engineering field, particularly, relate to a kind of methylotrophy type genus bacillus.
Background technology
Acetoin have another name called 3-Hydroxybutanone, vinegar drone, the methyl acetylcarbinol.Being present in dairy products and some fruit, is a kind of widely used flavouring agent, and it is edible that China GB 2760-86 stipulates that it allows.Acetoin is extremely extensive as the fragrance applications scope, and consumption is also larger, and its topmost effect is the production for spices such as cream, dairy products, liquor blending, sour milk and strawberry types.In addition, 3-Hydroxybutanone can also be widely used in other numerous industries as a kind of hardware and software platform compound, and USDOE was classified it as one of hardware and software platform compound of 30 kinds of preferential developments utilizations in 2004.
The production acetoin is mainly passed through chemical synthesis both at home and abroad at present, but there is the quality safety worry in it as flavouring agent.And microbial fermentation production acetoin has bioprocess technology environmental friendliness, product safety and the raw material advantage such as can regenerate, and will become the dominant direction that following acetoin is produced.At present domesticly biology is prepared acetoin mainly concentrate on the laboratory study development phases such as optimization that acetoin produces bacterium seed selection and conditions of flask fermentation, not yet enter commercial scale production.The generation bacterium of reporting mainly is subtilis etc., yet there are no the relevant report that methylotrophy type genus bacillus prepares acetoin, and the present invention provides new resources for microbe fermentation method prepares acetoin.
Summary of the invention
The present invention therefrom screen in the high-temperature daqu can the high yield acetoin bacterial strain, and it has been carried out the evaluation of morphology and genetics aspect, with this bacterium called after methylotrophy type genus bacillus (
Bacillus methylotrophicus), and it has been carried out preservation, and depositary institution is China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC), the preservation address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date is on April 18th, 2013, and deposit number is: CGMCC No.7485.
The invention provides a kind of methylotrophy type genus bacillus, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC), deposit number is: CGMCC No.7485.
The present invention also provides a kind of acetoin to produce bacterium, and it is methylotrophy type genus bacillus claimed in claim 1 that described acetoin produces bacterium.
The present invention also provides the above-mentioned application of methylotrophy type genus bacillus in the preparation acetoin.
Further, the concentration of the described acetoin for preparing is 15.71g/L.
Further, the step of described application is: produce acetoin take glucose as fermenting substrate methylotrophy type genus bacillus.
Further, the concentration of described glucose is 20-60g/L.
Further, the step of described application is: methylotrophy type genus bacillus with being cultured to logarithmic phase in the seed culture medium that contains 20-60g/L glucose, is seeded to it in fermention medium that contains 20-60g/L glucose, carries out shaking culture.
Further, the concentration of described glucose is 40g/L.
Use methylotrophy type genus bacillus of the present invention and ferment under certain condition, can obtain the acetoin that concentration is 15.71g/L.Through contrast, the throughput that acetoin that major part has been reported produces bacterium (bacterial strain that has is to transform through condition optimizing or breeding) is lower than or is equivalent to methylotrophy type genus bacillus CGMCC No.7485 of the present invention.Methylotrophy type genus bacillus CGMCC No.7485 among the present invention has been except having carried out the initial optimization glucose concn, and other culture condition and substratum form, and all to be that genus bacillus is commonly used select, and also not yet passes through the breeding transformation.Can infer that thus behind the methylotrophy type genus bacillus further breeding transformation of process of the present invention and the condition optimizing, acetoin output will be further enhanced, therefore, methylotrophy type genus bacillus of the present invention has broad application prospects.
Description of drawings
Fig. 1 be methylotrophy type genus bacillus CGMCC No.7485 bacterial strain of the present invention (
Bacillus methylotrophicus strain) the flat-plate bacterial colony photo;
Fig. 2 be methylotrophy type genus bacillus CGMCC No.7485 bacterial strain of the present invention (
Bacillus methylotrophicus strain) the gramstaining photo;
Fig. 3 be methylotrophy type genus bacillus CGMCC No.7485 bacterial strain of the present invention (
Bacillus methylotrophicus strain) the spore staining photo;
Fig. 4 be methylotrophy type genus bacillus CGMCC No.7485 bacterial strain of the present invention (
Bacillus methylotrophicus strain) 16Sr DNA cloning bands of a spectrum;
Fig. 5 be in the fermention medium of methylotrophy type genus bacillus CGMCC No.7485 bacterial strain of the present invention glucose content on the lab diagram that affects of acetoin output.
Embodiment
Embodiment 1: the screening of bacterial strain
Middle high-temperature daqu (take from Anhui two-wheel employment limited liability company) is ground to form powdery, accurately take by weighing in the Erlenmeyer flask that 25g joins the stroke-physiological saline solution that contains 200ml.The granulated glass sphere that adds sterilization is put under shaking table (180r/min) room temperature concussion and is broken up to take out behind the 30min and leave standstill 30min.Get the 1mL supernatant liquor and add the 9mL stroke-physiological saline solution, being mixed with volumetric concentration is 10
-1Bacteria suspension.The like, being mixed with volumetric concentration is 10
-3, 10
-410
-7Bacteria suspension, evenly coat cooling overnight isolation medium (preparation method of isolation medium is: with extractum carnis 3g/ L, peptone 10g/ L, NaCl 5g/ L, agar strip 20 g/ L, water 1000mL, nystatin 0.01g/ L mixes, transfer pH to 7.0,121 ℃, sterilization 20min.) on the flat board, the flat board that inoculation is good places 37 ℃ of constant incubators to cultivate 48h.The bacterial strain that all bacterium colonies are different is chosen and is purified to purebredly, and 4 ℃ of refrigerator inclined-planes are preserved.
Embodiment 2: the fermentation of bacterial strain
One, the fermentation of bacterial strain
(preparation method of seed culture medium is: with extractum carnis 20.0 g, glucose 20.0g, K from picking bacterial strain on the inclined-plane to seed culture medium
2HPO
41.0 g, (NH
4)
2SO
41.0 g, MgSO
47H
2O 1.0 g, NaCl 0.5 g, FeSO
47H
2O 0.01 g mixing, adding distil water is adjusted to 1L, transfers pH to 7.0, and 121 ℃, sterilization 20min.) in be cultured to logarithmic phase, will cultivate with seed culture medium the bacterial strain of logarithmic phase, by 5% inoculum size join fermention medium (preparation method of fermention medium is: with extractum carnis 20.0 g, glucose 20.0 g, K
2HPO
41.0 g, (NH
4)
2SO
41.0 g, MgSO
47H
2O 1.0 g, NaCl 0.5 g, FeSO
47H
2O 0.01 g mixing, adding distil water is adjusted to 1L, transfers pH to 7.0, and 121 ℃, sterilization 20min.) in, then place shaking culture 72h under 37 ℃, 150r/min condition.Collect fermented liquid in order to detecting.
Two, glucose content is as follows on the impact experiment of acetoin output in the fermention medium:
The picking bacterial strain is cultured to logarithmic phase to seed culture medium (it is the same to fill a prescription) from the inclined-plane, to cultivate with seed culture medium the bacterial strain of logarithmic phase, join by 5% inoculum size (other component prescription is the same except glucose) in the fermention medium that contains different glucose, wherein glucose concn is respectively 10 g/L, 20 g/L, 40 g/L, 60 g/L, 80 g/L, 100 g/L, then places shaking culture 72h under 37 ℃, 150r/min condition.Collect fermented liquid in order to detecting.As shown in Figure 5, acetoin content is up to 15.71g/L when glucose concn is 40g/L.
Embodiment 3: the detection of acetoin
The gas chromatographic detection condition: the fermented liquid of each different strains that embodiment 2 is obtained dilutes respectively 5 times, gets respectively afterwards 2 μ L sample introductions and carries out gas chromatographic analysis, detects the output of acetoin.That after testing, acetoin output is the highest is 15.71g/L.
Gas-chromatography parameter: liquor chromatographic column specially CP-Wax 57 C, internal diameter: 250.00 μ m, length: 50.0 m, thickness of liquid film: 0.20 μ m, dead time: 2.983 min, column temperature: 60 ℃ keep 5min, and 5 ℃/min temperature programming to 140 ℃ keeps 2min, injector temperature: 150 ℃, detector temperature: press 90kPa, tail to blow before 160 ℃, carrier gas: N2, post: 30mL/min, splitting ratio: 1:30, air: 50kPa, hydrogen: 50kPa.
Embodiment 4: the evaluation of bacterial strain
Select the highest bacterial strain (being that output is the bacterial strain of 15.71g/L) of acetoin output among the embodiment 3, its flat-plate bacterial colony photo, gramstaining photo and spore staining photo are respectively referring to Fig. 1 to Fig. 3.Take its genome as template, utilize the universal primer (16S27f and 16S1492r) of the 16S rDNA of bacterium to carry out pcr amplification.Amplified production is carried out genomic dna with 1% agarose gel electrophoresis separate, a bright band appears in the pcr amplification product of this bacterial strain, and its molecular size range is about 1500bp.See Fig. 4.Amplification is delivered to Sangon Biotech (Shanghai) Co., Ltd. check order, sequencing result is seen sequence 1 in the sequence table.Above-mentioned sequence is compared by RiboaptDB among blast program and the Genbank, find itself and methylotrophy type genus bacillus
(Bacillus methylotrophicus strain)16Sr dna sequence dna similarity be 99%.
It is delivered to China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) preservation on April 18th, 2013, and deposit number is: CGMCC No.7485.
From bibliographical information, can find out (Japanese plum ripple etc., microorganisms producing 3-Hydroxybutanone progress [J], biological processing, 2011:9 (6); Wang Meng, the preliminary study [D] of producing acetoin subtilis metabolic engineering, University Of Tianjin, 2012.6; Yin Minghao, the seed selection [D] of high yield 3-Hydroxybutanone subtilis, University Of Qingdao, 2011.5), the throughput that acetoin that major part has been reported produces bacterium (bacterial strain that has is to transform through condition optimizing or breeding) is lower than or is equivalent to methylotrophy type genus bacillus CGMCC No.7485 of the present invention.Methylotrophy type genus bacillus CGMCC No.7485 among the present invention has been except having carried out the initial optimization glucose concn, and other culture condition and substratum form, and all to be that genus bacillus is commonly used select, and also not yet passes through the breeding transformation.Can infer that thus behind the methylotrophy type genus bacillus further breeding transformation of process of the present invention and the condition optimizing, acetoin output will be further enhanced, therefore, methylotrophy type genus bacillus of the present invention has broad application prospects.
It should be noted that at last: the above only is the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment the present invention is had been described in detail, for a person skilled in the art, it still can be made amendment to the technical scheme that aforementioned each embodiment puts down in writing, and perhaps part technical characterictic wherein is equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Sequence table
＜110〉Anhui Polytechnic University
＜120〉a kind of methylotrophy type genus bacillus and application thereof
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1459
<212> DNA
＜213〉methylotrophy type genus bacillus
<400> 1
gcggagtctg ctgacatatc tgtcacgtcg tagcgagaca gatgggagct tgctccctga 60
tgttagcggc ggacgggtga gtaacacgtg ggtaacctgc ctgtaagact gggataactc 120
cgggaaaccg gggctaatac cggatggttg tttgaaccgc atggttcaga cataaaaggt 180
ggcttcggct accacttaca gatggacccg cggcgcatta gctagttggt gaggtaacgg 240
ctcaccaagg cgacgatgcg tagccgacct gagagggtga tcggccacac tgggactgag 300
acacggccca gactcctacg ggaggcagca gtagggaatc ttccgcaatg gacgaaagtc 360
tgacggagca acgccgcgtg agtgatgaag gttttcggat cgtaaagctc tgttgttagg 420
gaagaacaag tgccgttcaa atagggcggc accttgacgg tacctaacca gaaagccacg 480
gctaactacg tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc cggaattatt 540
gggcgtaaag ggctcgcagg cggtttctta agtctgatgt gaaagccccc ggctcaaccg 600
gggagggtca ttggaaactg gggaacttga gtgcagaaga ggagagtgga attccacgtg 660
tagcggtgaa atgcgtagag atgtggagga acaccagtgg cgaaggcgac tctctggtct 720
gtaactgacg ctgaggagcg aaagcgtggg gagcgaacag gattagatac cctggtagtc 780
cacgccgtaa acgatgagtg ctaagtgtta gggggtttcc gccccttagt gctgcagcta 840
acgcattaag cactccgcct ggggagtacg gtcgcaagac tgaaactcaa aggaattgac 900
gggggcccgc acaagcggtg gagcatgtgg tttaattcga agcaacgcga agaaccttac 960
caggtcttga catcctctga caatcctaga gataggacgt ccccttcggg ggcagagtga 1020
caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg 1080
agcgcaaccc ttgatcttag ttgccagcat tcagttgggc actctaaggt gactgccggt 1140
gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat gccccttatg acctgggcta 1200
cacacgtgct acaatggaca gaacaaaggg cagcgaaacc gcgaggttaa gccaatccca 1260
caaatctgtt ctcagttcgg atcgcagtct gcaactcgac tgcgtgaagc tggaatcgct 1320
agtaatcgcg gatcagcatg ccgcggtgaa tacgttcccg ggccttgtac acaccgcccg 1380
tcacaccacg agagtttgta acacccgaag tcggtgaggt aactagaagg agccagcgcg 1440
ctatatagag acaaccccg 1459
＜110〉Anhui Polytechnic University
＜120〉a kind of methylotrophy type genus bacillus and application thereof
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1459
<212> DNA
＜213〉methylotrophy type genus bacillus
<400> 1
gcggagtctg ctgacatatc tgtcacgtcg tagcgagaca gatgggagct tgctccctga 60
tgttagcggc ggacgggtga gtaacacgtg ggtaacctgc ctgtaagact gggataactc 120
cgggaaaccg gggctaatac cggatggttg tttgaaccgc atggttcaga cataaaaggt 180
ggcttcggct accacttaca gatggacccg cggcgcatta gctagttggt gaggtaacgg 240
ctcaccaagg cgacgatgcg tagccgacct gagagggtga tcggccacac tgggactgag 300
acacggccca gactcctacg ggaggcagca gtagggaatc ttccgcaatg gacgaaagtc 360
tgacggagca acgccgcgtg agtgatgaag gttttcggat cgtaaagctc tgttgttagg 420
gaagaacaag tgccgttcaa atagggcggc accttgacgg tacctaacca gaaagccacg 480
gctaactacg tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc cggaattatt 540
gggcgtaaag ggctcgcagg cggtttctta agtctgatgt gaaagccccc ggctcaaccg 600
gggagggtca ttggaaactg gggaacttga gtgcagaaga ggagagtgga attccacgtg 660
tagcggtgaa atgcgtagag atgtggagga acaccagtgg cgaaggcgac tctctggtct 720
gtaactgacg ctgaggagcg aaagcgtggg gagcgaacag gattagatac cctggtagtc 780
cacgccgtaa acgatgagtg ctaagtgtta gggggtttcc gccccttagt gctgcagcta 840
acgcattaag cactccgcct ggggagtacg gtcgcaagac tgaaactcaa aggaattgac 900
gggggcccgc acaagcggtg gagcatgtgg tttaattcga agcaacgcga agaaccttac 960
caggtcttga catcctctga caatcctaga gataggacgt ccccttcggg ggcagagtga 1020
caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg 1080
agcgcaaccc ttgatcttag ttgccagcat tcagttgggc actctaaggt gactgccggt 1140
gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat gccccttatg acctgggcta 1200
cacacgtgct acaatggaca gaacaaaggg cagcgaaacc gcgaggttaa gccaatccca 1260
caaatctgtt ctcagttcgg atcgcagtct gcaactcgac tgcgtgaagc tggaatcgct 1320
agtaatcgcg gatcagcatg ccgcggtgaa tacgttcccg ggccttgtac acaccgcccg 1380
tcacaccacg agagtttgta acacccgaag tcggtgaggt aactagaagg agccagcgcg 1440
ctatatagag acaaccccg 1459
Claims (8)
1. a methylotrophy type genus bacillus is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC), and deposit number is: CGMCC No.7485.
2. an acetoin produces bacterium, and it is methylotrophy type genus bacillus claimed in claim 1 that described acetoin produces bacterium.
3. the application of methylotrophy type genus bacillus claimed in claim 1 in the preparation acetoin.
4. application according to claim 3 is characterized by: the concentration of the described acetoin for preparing is 15.71g/L.
5. application according to claim 3 is characterized by: the step of described application is: produce acetoin take glucose as fermenting substrate methylotrophy type genus bacillus.
6. application according to claim 5 is characterized by: the concentration of described glucose is 20-60g/L.
7. application according to claim 6, it is characterized by: the step of described application is: be with being cultured to logarithmic phase in the seed culture medium that contains 20-60g/L glucose with methylotrophy type genus bacillus, it is seeded in the fermention medium that contains 20-60g/L glucose, carries out shaking culture.
8. according to claim 6 or 7 described application, it is characterized by: the concentration of glucose is 40g/L in the described fermention medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310265937.7A CN103361288B (en) | 2013-06-28 | 2013-06-28 | Methylotrophic bacillus and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310265937.7A CN103361288B (en) | 2013-06-28 | 2013-06-28 | Methylotrophic bacillus and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103361288A true CN103361288A (en) | 2013-10-23 |
| CN103361288B CN103361288B (en) | 2014-12-17 |
Family
ID=49363556
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310265937.7A Active CN103361288B (en) | 2013-06-28 | 2013-06-28 | Methylotrophic bacillus and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103361288B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017750A (en) * | 2014-04-14 | 2014-09-03 | 青岛海大生物集团有限公司 | Salt-resistance Bacillusmethylotrophicus strain LJ and application thereof |
| CN105112327A (en) * | 2015-08-20 | 2015-12-02 | 郑磊 | Method for separating bacilli and method for manufacturing fermented tea by aid of bacilli |
| CN110923163A (en) * | 2019-11-22 | 2020-03-27 | 郑州轻工业大学 | Bacillus methylotrophicus and application thereof |
| CN111235067A (en) * | 2020-03-16 | 2020-06-05 | 河南仰韶酒业有限公司 | White spirit enhanced yeast for making hard liquor, preparation method and application thereof |
| CN113430147A (en) * | 2021-07-30 | 2021-09-24 | 千禾味业食品股份有限公司 | Bacillus villagens QH-20011 with low pH tolerance and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294143A (en) * | 2008-06-20 | 2008-10-29 | 南京工业大学 | Bacterial strain for preparing 3-hydroxy butanone and uses thereof |
| CN102634563A (en) * | 2011-10-19 | 2012-08-15 | 江南大学 | Screening method of strain capable of producing acetoin and acetoin production method based on strain fermenting method |
| CN102732469A (en) * | 2012-07-12 | 2012-10-17 | 云南农业大学 | Bacillus methylotrophicus and application thereof |
| CN103074272A (en) * | 2012-12-17 | 2013-05-01 | 漳州三炬生物科技有限公司 | Bacillus methylotrophicus Sanju-04 and its application |
-
2013
- 2013-06-28 CN CN201310265937.7A patent/CN103361288B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294143A (en) * | 2008-06-20 | 2008-10-29 | 南京工业大学 | Bacterial strain for preparing 3-hydroxy butanone and uses thereof |
| CN102634563A (en) * | 2011-10-19 | 2012-08-15 | 江南大学 | Screening method of strain capable of producing acetoin and acetoin production method based on strain fermenting method |
| CN102732469A (en) * | 2012-07-12 | 2012-10-17 | 云南农业大学 | Bacillus methylotrophicus and application thereof |
| CN103074272A (en) * | 2012-12-17 | 2013-05-01 | 漳州三炬生物科技有限公司 | Bacillus methylotrophicus Sanju-04 and its application |
Non-Patent Citations (2)
| Title |
|---|
| 张燎原 等: "以葡萄糖和木糖为双底物生物合成乙偶姻的条件优化", 《化学与生物工程》, vol. 29, no. 7, 31 December 2012 (2012-12-31) * |
| 罗建超 等: "大曲中产酱香芽孢杆菌的筛选及其代谢产香探析", 《酿酒科技》, no. 5, 31 December 2012 (2012-12-31) * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017750A (en) * | 2014-04-14 | 2014-09-03 | 青岛海大生物集团有限公司 | Salt-resistance Bacillusmethylotrophicus strain LJ and application thereof |
| CN104017750B (en) * | 2014-04-14 | 2016-03-02 | 青岛海大生物集团有限公司 | A kind of salt tolerance Methylotrophic Bacillus strain LJ and application thereof |
| CN105112327A (en) * | 2015-08-20 | 2015-12-02 | 郑磊 | Method for separating bacilli and method for manufacturing fermented tea by aid of bacilli |
| CN110923163A (en) * | 2019-11-22 | 2020-03-27 | 郑州轻工业大学 | Bacillus methylotrophicus and application thereof |
| CN110923163B (en) * | 2019-11-22 | 2021-06-25 | 郑州轻工业大学 | Bacillus methylotrophicus and application thereof |
| CN111235067A (en) * | 2020-03-16 | 2020-06-05 | 河南仰韶酒业有限公司 | White spirit enhanced yeast for making hard liquor, preparation method and application thereof |
| CN111235067B (en) * | 2020-03-16 | 2021-10-01 | 河南仰韶酒业有限公司 | White spirit enhanced yeast for making hard liquor, preparation method and application thereof |
| CN113430147A (en) * | 2021-07-30 | 2021-09-24 | 千禾味业食品股份有限公司 | Bacillus villagens QH-20011 with low pH tolerance and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103361288B (en) | 2014-12-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103361288B (en) | Methylotrophic bacillus and application thereof | |
| CN110643544B (en) | Lactobacillus paracasei761, application thereof, silage additive and silage | |
| CN110591987B (en) | Lactobacillus salivarius358 and application thereof, silage additive and silage | |
| CN107746861B (en) | Biological preparation method of (R) -1- (2-trifluoromethylphenyl) ethanol | |
| CN106554931B (en) | Carboxylic acid bacterium bailii and application thereof | |
| CN106399195B (en) | A kind of Lactobacillus casei and its application | |
| Ling et al. | Assessing the microbial communities in four different daqus by using PCR-DGGE, PLFA, and biolog analyses | |
| CN103333842B (en) | Bacillus subtilis producing 3-hydroxybutanone and application thereof | |
| CN103451133A (en) | Bacillus circulans and application for same in preparation for ferulic acid decarboxylase | |
| CN110042072B (en) | Aflatoxin B degradation1And application thereof | |
| CN111411061A (en) | Screening of bacillus licheniformis and application of bacillus licheniformis in food production | |
| CN110684691A (en) | Preparation process of microbial agent based on directional screening of microorganisms | |
| CN101864381A (en) | Breeding of microbial strain for producing 3-hydroxy-2-butanone by fermenting substrate glucose | |
| CN105713851B (en) | One plant of Clostridium beijerinckii and its application | |
| CN111621432B (en) | Bacillus licheniformis, screening method and application | |
| Lü et al. | Characterization of the effective cellulose degrading strain CTL-6 | |
| CN103451123B (en) | Large coccus of molten junket and its preparation method and application | |
| CN103468606B (en) | Klebsiella oxytoca and application thereof in allitol production | |
| CN114075520B (en) | Bacillus belicus for producing gamma-polyglutamic acid and method for producing gamma-polyglutamic acid by solid fermentation of bacillus belicus | |
| CN111592999B (en) | Bacillus simplex and application thereof in producing low-temperature amylase | |
| CN110835610B (en) | Composite microbial inoculum suitable for degrading straw and preparation method thereof | |
| CN109134125A (en) | A kind of method that biological reinforced compost quickly prepares soil conditioner | |
| CN105647812A (en) | Schizophyllum commune strain and application thereof in production of Pu'er tea | |
| CN102776128B (en) | Microbial compound bacteria agent for treating oilfield fracturing flowback fluid, and preparation method of microbial compound agent | |
| CN105296379A (en) | Pseudonocardia sp. and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20211126 Address after: 266706 No. 299, Beihai Road, Tonghe sub district office, Pingdu City, Qingdao City, Shandong Province Patentee after: Qingdao Northen Biotechnology Co.,Ltd. Address before: 241081 room 228, building 2, Chuangye street, No. 8, Longhu street, Sanshan District, Wuhu City, Anhui Province Patentee before: WUHU YANGZHAN NEW MATERIALS TECHNOLOGY SERVICE Co.,Ltd. Effective date of registration: 20211126 Address after: 241081 room 228, building 2, Chuangye street, No. 8, Longhu street, Sanshan District, Wuhu City, Anhui Province Patentee after: WUHU YANGZHAN NEW MATERIALS TECHNOLOGY SERVICE Co.,Ltd. Address before: College of biological and chemical engineering, Anhui University of Technology Patentee before: ANHUI POLYTECHNIC University |