CN109134125A - A kind of method that biological reinforced compost quickly prepares soil conditioner - Google Patents

A kind of method that biological reinforced compost quickly prepares soil conditioner Download PDF

Info

Publication number
CN109134125A
CN109134125A CN201710454866.3A CN201710454866A CN109134125A CN 109134125 A CN109134125 A CN 109134125A CN 201710454866 A CN201710454866 A CN 201710454866A CN 109134125 A CN109134125 A CN 109134125A
Authority
CN
China
Prior art keywords
soil conditioner
soil
biological reinforced
quickly prepares
compost
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710454866.3A
Other languages
Chinese (zh)
Inventor
郭亮
邓飞
赵涛
徐刚
吴明红
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Shanghai for Science and Technology
Original Assignee
University of Shanghai for Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Shanghai for Science and Technology filed Critical University of Shanghai for Science and Technology
Priority to CN201710454866.3A priority Critical patent/CN109134125A/en
Publication of CN109134125A publication Critical patent/CN109134125A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F9/00—Fertilisers from household or town refuse
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Fertilizers (AREA)

Abstract

The present invention relates to a kind of methods that biological reinforced compost quickly prepares soil conditioner, pretreated organic solid waste will be carried out as composting material, the microbe inoculation composite bacteria agent A when compost starts, fermentation reactor system is carried out after mixing, when heap temperature is warming up to 50~55 DEG C, microbe inoculation composite bacteria agent B continues to ferment after mixing, when temperature is down to 20~30 DEG C, obtained windrow is the soil conditioner.Compared with prior art, the present invention had not only retained positive effect of the indigenous microorganism in compost, but also had given full play to the specific function of external microorganism.In addition to this, humification 7d windrow is applied in soil, by soil carbon content while shortening process cycle, improves soil quality.

Description

A kind of method that biological reinforced compost quickly prepares soil conditioner
Technical field
The present invention relates to soil fertility to improve field, quickly prepares soil conditioning more particularly, to a kind of biological reinforced compost The method of agent.
Background technique
Soil conditioner can promote soil granular formation, improvement soil texture, build up fertility, protect topsoil soils, Improve soil water-reataining fertilizer-keeping, improve grain yield.Compost is one of the main method of reclaiming organic waste processing, soil Middle application organic compost can increase soil organic matter amount, improve soil property.For the long problem of Traditional compost fermentation period, Studies have shown that organic matter degradation can be accelerated by microbe inoculation microbial inoculum, composting efficiency and quality of compost products are improved.And it is another On the one hand, at soil degradation, three one-tenth barren, two one-tenth heavy metal pollutions, the soil organism is seriously deficient in China four, nearly 30 years soil Carbon reduces 50%, C/N ratio serious unbalance, and soil pollution and food-safety problem aggravate, and urgent need is gone out organic from soil Matter returns to soil, maintains carbon, nitrogen balance, reduces agricultural non-point source pollution.
Summary of the invention
It is an object of the present invention to overcome the above-mentioned drawbacks of the prior art and provide a kind of biological reinforced compost The method for quickly preparing soil conditioner.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of method that biological reinforced compost quickly prepares soil conditioner, will carry out pretreated organic solid waste As composting material, the microbe inoculation composite bacteria agent A when compost starts carries out fermentation reactor system after mixing, to heap temperature liter When temperature is to 50~55 DEG C, microbe inoculation composite bacteria agent B continues to ferment after mixing, when temperature is down to 20~30 DEG C, obtained heap Material is the soil conditioner.
The composite bacteria agent A is by two kinds of pseudomonads, two kinds of bacillus, Yi Zhongwei as a preferred technical solution, The Dysgonomonas of culture, a kind of Lactobacillus salivarius and a kind of Aeribacillus pallidus composition.
Two kinds of pseudomonads are respectively bacillus cereus and lichens gemma bar as a preferred technical solution, Bacterium, two kinds of bacillus are respectively Pseudomonas caeni and Pseudomonas sp..
As a preferred technical solution, pseudomonad in the complex microbial inoculum A, bacillus, do not cultivate The ratio between concentration of Dysgonomonas, Lactobacillus salivarius and Aeribacillus pallidus is 2:1:2:1.5:1.
The complex microbial inoculum B is lignocellulose degrading bacteria as a preferred technical solution, in high temperature Lower lignocellulose degradation.
The complex microbial inoculum B is made of 20 plants of bacteriums and 10 fungal strains as a preferred technical solution,.
20 plants of bacteriums are thermophilic bacteria as a preferred technical solution, belong to Bacillales；Described 10 Fungal strain is Penicillium sp., comes from Eurotiomycetes guiding principle.
The concentration of each bacterium in the complex microbial inoculum B and fungi is equal as a preferred technical solution,.
The complex microbial inoculum A and complex microbial inoculum B are with the shape of bacterium solution as a preferred technical solution, Formula inoculation, and be inoculated in 1.25mL logarithmic phase bacterium solution/kg composting material siccative ratio.
The concentration of the bacterium solution is 1 × 10 as a preferred technical solution,8cfu/ml。
The organic solid waste is fresh kitchen castoff as a preferred technical solution,；The pretreatment Are as follows: by the broken dehydration of fresh kitchen castoff, addition rice husk adjustment moisture content is 50~60%, C/N between 20~30.
The present invention is inoculated in compost different phase, improves degree of humification；Pass through regulation activity organic carbon and inertia Organic carbon ratio improves soil microbial activities, and then promotes soil fertility.
Edaphon is the main promotion of the important component and soil material circulation and energy flow of soil , the formation and decomposition of humus are equally the results of microbial activities.It is poor that different compost period biological community structures exist Different, inoculation can give full play to the effect of each stage microorganism stage by stage.Compost initial stage, easily biodegradable organics take the lead in by microorganism It utilizes, with the raising of compost temperature, complicated hardly degraded organic substance starts to decompose, and promotes humus to be formed, inertia organic carbon contains Amount increases.Active organic carbon in soil can be obviously improved soil fertility, and inertia organic carbon is conducive to soil physical properties improvement, improves Soil carbon sequestration capacity using.Therefore it can be made up of, and then realize to soil fertilizer at humus is regulated and controled with environmental condition microorganism group The regulation of power and soil carbon sequestration.The compost material for having reached innoxious standard, humification 7d is applied in soil, the work easily utilized Property organic carbon is quickly utilized, and significantly improves soil microbial activities, improves lean soil quality.
Specifically, complex microbial inoculum A was added in the initial stage of compost.In kitchen castoff composting process In, the main reason for causing organic matter degradation slow is the presence of the small molecular organic acid of high concentration, mainly lactic acid and acetic acid.? Under low ph condition, small molecular organic acid mainly exists in the form of not dissociating, does not dissociate form to the toxic effect of microorganism, thus Compost microbe can be inhibited active.From kitchen castoff compost acidification the inhibition phase screening to kitchen castoff compost environment have compared with Anaerobic digestion composite microbial system --- the complex microbial inoculum A of strong adaptability, in the compost initial vaccination microbial inoculum, pH is whole Persistently increased in a composting cycle, there is no acidification suppression, accelerate the heating of compost, compost 32h reaches 55 DEG C, quickly into Enter high temperature fermentation period.
Contain a certain amount of lignin and cellulose in kitchen castoff composting material, and this substance is in composting process Compared with difficult for biological degradation.Complex microbial inoculum B is lignocellulose degrading bacteria, is used for lignocellulose degradation at high temperature, The addition of compost hot stage, and it is quickly become dominant microflora, to accelerate the degradation of lignocellulosic, promote the quick corruption of compost It is ripe.Since complex microbial inoculum B mainly works in hot stage, cool period activity is compared with so in temperature heap temperature It is added after being increased to 50~55 DEG C, guarantees degradation effect.
Experiment enters megathermal period (> 55 DEG C) after showing microbe inoculation composite bacteria agent A, 32h and continues 5d, meets compost Innoxious requirement.After compost 7d, temperature is down to 30 DEG C hereinafter, windrow is applied in lean soil at this time.It is added in composting process Complex microbial inoculum A be remarkably improved small molecular organic acid degrading microorganism diversity and abundance, so as to avoid acid it is right The harm of soil crop；Complex microbial inoculum B accelerates ligocellulose degradation, improves compost degree of humification.Lean soil Middle microorganism is in starvation, and windrow is applied in soil, and active organic carbon is quickly utilized by edaphon, microbial activity Rise with quantity, increases soil fertility and crop yield；Inertia organic carbon improves soil physical properties, improves fertilizer-preserving ability.
Compared with prior art, the present invention studies biology in conjunction with composting process by soil organic matter trans-utilization mechanism Strengthen humification soil conditioner preparation method, be inoculated in compost different phase, improves degree of humification；It is lived by regulation Property organic carbon and inertia organic carbon ratio improve soil microbial activities, and then promote soil fertility.Both retain indigenous microorganism Positive effect in compost, and given full play to the specific function of external microorganism.In addition to this, humification 7d windrow is applied Into soil, by soil carbon content while shortening process cycle, improve soil quality.
Detailed description of the invention
Fig. 1 is heap temperature change curve in composting process；
Fig. 2 is composting device schematic diagram of the invention.
In figure, 1 is air pump, and 2 be gas flowmeter, and 3 be heap body, and 4 be temperature difference control mechanism, and 5 be insulating box, and 6 be gas Analyzer, 7 be filter mechanism.
Specific embodiment
The present invention is described in detail with specific embodiment below in conjunction with the accompanying drawings.
Embodiment 1
A kind of method that biological reinforced compost quickly prepares soil conditioner, comprising the following steps:
(1) fresh kitchen castoff is taken, after broken dehydration, addition rice husk adjusts moisture content 50%-60%, and C/N exists Between 20~30, composting material is obtained.
(2) 1.25mL logarithmic phase bacterium solution/kg composting material dry matter inoculum concentration microbe inoculation composite bacteria agent A is pressed, is mixed It closes uniformly, spontaneous fermentation is carried out in composting device, Fig. 1 is shown in heap temperature variation, from figure 1 it appears that microbe inoculation After composite bacteria agent A, the heating of heap body is significantly faster than that (control group is kitchen waste compost processing group to control group, is not connect in composting process Kind compound bacteria A and B, make it heat up naturally).
(3) it after heap temperature rises to 50 DEG C~55 DEG C, in ratio inoculating compound bacterium agent B in step (2), is uniformly mixed, Continue to ferment.
(4) after second of inoculation, 5-7d is banked up, after temperature is down to 20~30 DEG C, obtained windrow is the soil Earth conditioner can be applied in soil.
The complex microbial inoculum A of the present embodiment is not cultivated by two kinds of pseudomonads, two kinds of bacillus, one kind Dysgonomonas, a kind of Lactobacillus salivarius and a kind of Aeribacillus pallidus composition.Wherein, pseudomonad, gemma The ratio between bacillus, concentration of the Dysgonomonas not cultivated, Lactobacillus salivarius and Aeribacillus pallidus are 2:1:2: 1.5:1, two kinds of pseudomonads are respectively bacillus cereus and bacillus licheniformis, and two kinds of bacillus are respectively Pseudomonas caeni and Pseudomonas sp..The complex microbial inoculum B of the present embodiment is ligocellulose degradation Bacterium is made of, the concentration phase of each bacterium and fungi for lignocellulose degradation at high temperature 20 plants of bacteriums and 10 fungal strains Deng.
In the present embodiment, the concentration of complex microbial inoculum A and complex microbial inoculum B are 1 × 108cfu/ml。
Composting device in the present embodiment is shown in that Fig. 2, reactor outlet gas analyzer 6 are connected, and monitors O on-line2-H2S、CO2 Gas concentration, exit gas are discharged after the filtering of filter mechanism 7.The oxygen supply of heap body 3 is controlled by air pump 1 and gas flowmeter 2, out For mouth oxygen concentration general control in 5%~15% range, speed of oxygen supply is set to 0.8L/minkg as no particular case Volatile organic matter.Stack layers gas permeability is by H2The control of S gas concentration, once H2S volume fraction be more than certain numerical value (such as 5 × 10-6), stack layers need to stir again.By temperature difference control mechanism 4 and 5 control system temperature of insulating box control at 65 DEG C hereinafter, A part of heat can be discharged by increasing gas supply in automatic alarm when more than 65 DEG C, and humid control is (entire in a certain range Composting process can control 58.5%), can take out a small amount of sample from reactor daily, carry out biochemical analysis.
Using method of the invention, micro organism quantity is obviously increased in windrow, the addition of complex microbial inoculum A, is inhibited Compost initial stage is acidified inhibiting effect, promotes the rapid increase of temperature, and compost 32h enters the megathermal period, adds high temperature lignocellulosic Degradation bacteria (complex microbial inoculum B) helps to maintain high temperature, reaches innoxious standard.Studies have shown that crop yield and activity Organic carbon is significantly positively correlated, and illustrates active organic carbon in soil and carbon cycle is ensure fertility and sustainable development important Factor.Active organic carbon in soil can promote soil carbon cycle to increase fertility, and then increase crop yield.Windrow is applied to In soil, active organic carbon component is utilized by Soil Microorganism, increases soil microbe quantity and activity；In addition, inoculation is micro- Bacteria agent significantly improves compost degree of humification, and inertia organic carbon (humus united) relative increase is conducive to soil physical property Matter improves, and improves soil carbon sequestration capacity using.Windrow is substantially reduced in the technique top fermentation time, lean soil product can be obviously improved Matter is a kind of conditioner preparation method of fast lifting lean soil quality.

Claims (10)

1. a kind of method that biological reinforced compost quickly prepares soil conditioner, which is characterized in that will carry out pretreated having For machine solid waste as composting material, the microbe inoculation composite bacteria agent A when compost starts carries out fermentation reactor system after mixing, to When heap temperature is warming up to 50~55 DEG C, microbe inoculation composite bacteria agent B continues to ferment after mixing, and temperature is down to 20~30 DEG C When, obtained windrow is the soil conditioner.
2. the method that a kind of biological reinforced compost according to claim 1 quickly prepares soil conditioner, which is characterized in that The composite bacteria agent A is by two kinds of pseudomonads, two kinds of bacillus, a kind of Dysgonomonas not cultivated, a kind of saliva Lactobacillus and a kind of Aeribacillus pallidus composition.
3. the method that a kind of biological reinforced compost according to claim 2 quickly prepares soil conditioner, which is characterized in that Two kinds of pseudomonads are respectively bacillus cereus and bacillus licheniformis, and two kinds of bacillus are respectively Pseudomonas caeni and Pseudomonas sp..
4. the method that a kind of biological reinforced compost according to claim 2 quickly prepares soil conditioner, which is characterized in that Pseudomonad in the complex microbial inoculum A, bacillus, the Dysgonomonas not cultivated, Lactobacillus salivarius and The ratio between concentration of Aeribacillus pallidus is 2:1:2:1.5:1.
5. the method that a kind of biological reinforced compost according to claim 1 quickly prepares soil conditioner, which is characterized in that The complex microbial inoculum B is lignocellulose degrading bacteria, for lignocellulose degradation at high temperature.
6. the method that a kind of biological reinforced compost according to claim 4 quickly prepares soil conditioner, which is characterized in that The complex microbial inoculum B is made of 20 plants of bacteriums and 10 fungal strains.
7. the method that a kind of biological reinforced compost according to claim 6 quickly prepares soil conditioner, which is characterized in that The concentration of each bacterium and fungi in the complex microbial inoculum B is equal.
8. the method that a kind of biological reinforced compost according to claim 1 quickly prepares soil conditioner, which is characterized in that The complex microbial inoculum A and complex microbial inoculum B is inoculated in the form of bacterium solution, and presses 1.25mL logarithmic phase The inoculation of bacterium solution/kg composting material siccative ratio.
9. the method that a kind of biological reinforced compost according to claim 8 quickly prepares soil conditioner, which is characterized in that The concentration of the bacterium solution is 1 × 108cfu/ml。
10. the method that a kind of biological reinforced compost according to claim 1 quickly prepares soil conditioner, feature exist In the organic solid waste is fresh kitchen castoff；The pretreatment are as follows: be crushed fresh kitchen castoff Dehydration, addition rice husk adjustment moisture content is 50~60%, C/N between 20~30.
CN201710454866.3A 2017-06-16 2017-06-16 A kind of method that biological reinforced compost quickly prepares soil conditioner Pending CN109134125A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710454866.3A CN109134125A (en) 2017-06-16 2017-06-16 A kind of method that biological reinforced compost quickly prepares soil conditioner

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710454866.3A CN109134125A (en) 2017-06-16 2017-06-16 A kind of method that biological reinforced compost quickly prepares soil conditioner

Publications (1)

Publication Number Publication Date
CN109134125A true CN109134125A (en) 2019-01-04

Family

ID=64830303

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710454866.3A Pending CN109134125A (en) 2017-06-16 2017-06-16 A kind of method that biological reinforced compost quickly prepares soil conditioner

Country Status (1)

Country Link
CN (1) CN109134125A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114736833A (en) * 2022-05-17 2022-07-12 浙江大学 High-temperature-resistant degradation microbial inoculum for household kitchen waste compost and method thereof
CN114920594A (en) * 2022-06-30 2022-08-19 北京科技大学 Method for co-composting kitchen waste and biogas residues and application thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114736833A (en) * 2022-05-17 2022-07-12 浙江大学 High-temperature-resistant degradation microbial inoculum for household kitchen waste compost and method thereof
CN114736833B (en) * 2022-05-17 2023-10-20 浙江大学 High-temperature-resistant degradation microbial inoculum for household kitchen waste compost and method thereof
CN114920594A (en) * 2022-06-30 2022-08-19 北京科技大学 Method for co-composting kitchen waste and biogas residues and application thereof

Similar Documents

Publication Publication Date Title
Zhao et al. Effect of thermo-tolerant actinomycetes inoculation on cellulose degradation and the formation of humic substances during composting
CN103951474B (en) Pig manure fermentation fertilizer and preparation method thereof
CN101333510B (en) Method for processing sludge and preparing bio organic fertilizer and special leaven thereof
CN105002221B (en) The method of intensive Vegetable area reclaiming organic waste efficient anaerobic fermentation production biogas
CN104293694A (en) Preparation method for sludge aerobic composting composite inoculum
CN103898032A (en) Complex microbial inoculant and method for preparing organic fertilizer by using same
CN105418172A (en) Preparation method of biological organic fertilizer
CN102701838B (en) Microbial organic fertilizer by using papermaking sludge as raw material and preparation method thereof
CN107176891A (en) It is a kind of to promote the biological agent and its production technology of stalk fast degradation
Kausar et al. Use of lignocellulolytic microbial consortium and pH amendment on composting efficacy of rice straw
CN102432355A (en) Banana stem organic fertilizer and preparation method thereof
CN113444657A (en) Solid-state microbial composite microbial agent for promoting aerobic composting of kitchen waste and preparation and application thereof
Ventorino et al. Chestnut biomass biodegradation for sustainable agriculture
Gaind Exploitation of orange peel for fungal solubilization of rock phosphate by solid state fermentation
CN101696391B (en) Rapid composting microbial inoculum of agricultural wastes and method for preparing organic fertilizer from the same
CN103695338B (en) A kind of Compound microbial activator and preparation method thereof and application
CN106396783A (en) Method for preparing organic fertilizer from grape peel pomace
CN110627543B (en) Method for promoting compost maturity of livestock and poultry manure through microbial pretreatment
Wan et al. Screening of lignin-degrading fungi and bioaugmentation on the directional humification of garden waste composting
CN108277187B (en) Microbial agent for efficient biological drying of municipal sludge
CN111574254A (en) Production process for fermenting organic fertilizer by utilizing Maotai-flavor liquor vinasse
CN105503295A (en) Special culture medium for blueberry and production process thereof
CN109134125A (en) A kind of method that biological reinforced compost quickly prepares soil conditioner
CN112625921B (en) Bacterium preparation for treating waste with high lignin content
CN106350504A (en) Straw low temperature degradation acidification microbial agent and preparation method and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20190104

WD01 Invention patent application deemed withdrawn after publication