CN106396783A - Method for preparing organic fertilizer from grape peel pomace - Google Patents
Method for preparing organic fertilizer from grape peel pomace Download PDFInfo
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- CN106396783A CN106396783A CN201610386036.7A CN201610386036A CN106396783A CN 106396783 A CN106396783 A CN 106396783A CN 201610386036 A CN201610386036 A CN 201610386036A CN 106396783 A CN106396783 A CN 106396783A
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- grape skin
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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Abstract
The invention discloses a method for preparing organic fertilizer from grape peel pomace. The method overcomes the problem of environmental pollution of the grape peel pomace and maximally utilizes the waste resource. The method comprises the following steps: subjecting 1, 000 kg of grape peel pomace to air drying, seed removal and crushing successively; then adding 2% of urea; adjusting humidity to 60 to 70%; inoculating the grape peel pomace with 20% of a liquid strain composed of Phanerochaete chrysosporium, Geotrichum candidum and Aspergillus niger; inserting a thermometer into a fermentation pile; when the temperature of the center of the pile reaches 56 DEG C, turning the pile and supplementing water to adjust humidity to about 60 to 70%; and carrying out fermentation for 50 d or more so as to fully decompose the grape peel pomace.
Description
Technical field
The present invention relates to biological organic fertilizer develops field and in particular to a kind of grape skin produces the preparation method of fertilizer.
Technical background
China is agricultural production big country; with society to the pay attention to day by day of environmental protection and developing rapidly of the ecological agriculture; and consumer rises year by year to the demand of green organic food; the use of biological organic fertilizer etc. " green fertilizer " receives much concern, and its research and development uses and also will promote emerging " pollution-free industry ".
How fully to utilize existing solid waste, particularly it is rich in the debirs of the nutrient such as organic and a certain amount of nitrogen, phosphorus, potassium developing Bio-organic fertilizer technology, solve the problems, such as that in agricultural production, generally existing the agricultural environment pollution that excess applies chemical fertilizer, organic fertilizer application is not enough and leads to, agricultural product quality is not high it is ensured that the sustainable development of agricultural has become modern agriculture major issue urgently to be resolved hurrily.Additionally, development biological organic fertilizer is to provide crop alimentary, realize the needs of agricultural production and income, be also soil protection fertility with rural environment, the needs of realizing recycling economy.
China is one of big country that grape produces, annual production now just there are about more than 600 ten thousand tons, wherein 80% is used for making wine, and in grape wine process, the accessory substances such as substantial amounts of skin slag can be produced, account for the 25%～30% of Grape processing amount, processing the commonly used method of grape skin at present is to be considered as rubbish to topple over, serious waste of resources.Using Environmental Biotechnology principle, grape skin is converted into fertilizer, the problem of environmental pollution of discarded grape skin can be solved, maximally utilize waste resource, safeguard environmental benefit, bring new profit growth point for manufacturing enterprise, there is obvious Impacts on ecology and environment and economic benefit, also comply with the requirement that China develops a circular economy.Additionally, inherently one environment-friendly type product of fertilizer, Long-Time Service fertilizer has great benefit for soil and crops.This patent studies, for raw material, the method that microbe inoculation solid-state aerobic fermentation obtains fertilizer with grape skin, and is targetedly used for improving soil environment and soil texture, fertility etc. with reference to the characteristic of this product.As prior art Authorization Notice No.：The Chinese invention patent of CN103524160B, a kind of Azadirachtin bio-organic fertilizer of this disclosure of the invention, the biological organic fertilizer of this invention comprises the following components in percentage by weight：Print chinaberry is withered 15～80%, organic 15～80%, microbial bacterial agent 0.1～5%, stack temperature and be 35-50 DEG C.Add print chinaberry in this invention formula withered, can have certain inhibitory action to miscellaneous bacteria breeding, therefore, raw material need not carry out sterilization treatment；Meanwhile, the biological organic fertilizer that this invention is prepared is processed by fermentation, and the phenomenon not resulting in burn seedlings during use occurs, fool proof reliability.
Content of the invention
The technical problem to be solved is to provide the preparation method that a kind of craft science is reasonable, operate grape skin production fertilizer that is simple and solving problem of environmental pollution.
The present invention by solving the scheme taken of above-mentioned technical problem is：A kind of grape skin produces the preparation method of fertilizer it is characterised in that following steps：
1) grape skin be dried after remove grape pip after and pulverize；
2) by step 1) described in pulverizing Grape Skin be configured to water material mass ratio be 1-8：1-5, regulation humidity is 60-70%, and in heaps；
3) adjusting fluid nutrient medium pH value is 1-7；
4) add water to heap body, and in water, urea content is 1.0-6.0%；
5) preparation of liquid spawn：By Phanerochaete chrysosporium, geotrichum candidum, aspergillus niger 1-5:1-6:The ratio of 1-8 is inoculated in above-mentioned steps 3) described in fluid nutrient medium in carry out shaking table culture produce liquid spawn；
6) liquid spawn is inoculated in grape skin by the inoculum concentration of the mass ratio of 10-60%, stirs；
7) mixture is stacked fermentation, and heap body core temperature reaches 30-70 DEG C of turning, adjust humidity simultaneously；
8) in the presence of fungi mixed bacteria, grape skin fully becomes thoroughly decomposed and central temperature is gradually reduced, and shows fermentation ends, finally carries out follow-up granulation..
Preferably, described step 2) in yeasting water material mass ratio be 2.5：1.
Or, described step 2) in yeasting water material mass ratio be 3.5：2.
Or, described step 2) in yeasting water material mass ratio be 5.5：3.
Preferably, described step 3) in Medium's PH Value be 4.5.
Preferably, described step 4) in water urea content be 2.0%.
Or, described step 4) in water urea content be 3.5%.
Preferably, described step 3) in liquid spawn be prepared as Phanerochaete chrysosporium, geotrichum candidum, aspergillus niger 1:1:1 ratio is inoculated in fluid nutrient medium and carries out shaking table culture production liquid spawn.
Preferably, described step 6) in inoculum concentration be 20% processing.
Or, described step 6) in inoculum concentration be 35% processing.
Preferably, described step 7) in overthrow when accumulation center temperature reaches 56 DEG C.
Or, described step 7) in accumulation temperature overthrow when reach 60 DEG C.
Compared with prior art, advantages of the present invention：
1, the present invention relates to grape skin is applied to the production method of bio-feritlizer, is converted to grape skin using composite bacteria, produces green organic fertilizer.
2nd, this technique can make the waste residue of Production of Wine be utilized, and realizes making full use of of resource while mitigating environmental pollution, the product of gained for the security improving agricultural product significant it is ensured that the sustainable development of agricultural.
Specific embodiment
With reference to embodiments the present invention is described in further detail.
Embodiment 1：A kind of grape skin produces the method for fertilizer it is characterised in that following steps：
(1) fermented bacterium selects：By using the impact to skin slag fermenting and producing fertilizer of single culture and composite bacteria, to screen the bacterial classification of suitable grape skin solid state fermentation.Experimental result such as table 1：In single culture, the process ferment effect of white-rot fungi is preferable, its content of humic acid, total nitrogen content highest；White-rot fungi, geotrichum candidum, the Combined Processing content of humic acid of aspergillus niger, full nitrogen, the equal highest of content of tatal phosphorus in composite bacteria, and full nitrogen, content of tatal phosphorus are higher than that single culture is processed.Consider available white-rot fungi, geotrichum candidum, the combination of aspergillus niger are used as the microbial inoculum that fermented grape skin slag produces fertilizer.
| Humic acid (%) | Full nitrogen (%) | Full phosphorus (%) | |
| Control group A | 95 | 87 | 63 |
| Control group B | 93 | 85 | 76 |
| Control group C | 84 | 79 | 65 |
| Experimental group | 93 | 92 | 87 |
1 four kinds of bacterial classification scheme comparison's results of table
(2) determination of inoculum concentration：By investigating the impact to humic acid, full nitrogen and content of tatal phosphorus during grape skin fermentation fertilizer processed for the different vaccination amount.Test result indicate that inoculum concentration is 20% process, content of humic acid, content of tatal phosphorus highest, total nitrogen content is higher.
(3) determination of yeasting condition：The impact to grape skin fermentation fertilizer processed of research ratio of water to material, Medium's PH Value, urea content.Result shows：Through the humic acid in comprehensive analysis grape skin fertilizer, full nitrogen, full phosphorus content, fermentation condition conveniently is 2.5 for ratio of water to material：1st, Medium's PH Value be 4.5, urea content be 2.0%.
(4) management in sweat：During solid-state compost fermentation, the accumulation due to temperature raises, central temperature is up to more than 60 DEG C, for ensureing the abundant conversion in fermentation mesostroma, necessary turning can ensure that ventilation and avoids that temperature is too high to have influence on internal mycelial growth, and the appearance features combining during turning determine the process of fermentation.
(5) determination of fermentation termination：When fermentation is close to terminal, the temperature at fermentation center starts to be gradually reduced, and now opens matrix central authorities and can find that mycelium is gradually decayed, shows fermentation ends, now can carry out the demand to meet market for the follow-up granulation.
Embodiment 2：
1st, a kind of grape skin produces the method for fertilizer it is characterised in that following steps：
1) grape skin be dried after remove grape pip after and pulverize, can more fully ferment；
2) by step 1) described in pulverizing Grape Skin be configured to water material mass ratio be 1-8：1-5, regulation humidity is 60-70%, and in heaps；
3) adjusting fluid nutrient medium pH value is 1-7；
4) add water to heap body, and in water, urea content is 1.0-6.0%；
5) preparation of liquid spawn：By Phanerochaete chrysosporium, geotrichum candidum, aspergillus niger 1-5:1-6:The ratio of 1-8 is inoculated in step 3) described in fluid nutrient medium in carry out shaking table culture produce liquid spawn；
6) liquid spawn is inoculated in grape skin by the inoculum concentration of the mass fraction of 10-60%, stirs；
7) mixture is stacked fermentation, and heap body core temperature reaches 30-70 DEG C of turning, adjust humidity simultaneously；
8) in the presence of fungi mixed bacteria, grape skin fully becomes thoroughly decomposed and central temperature is gradually reduced, and shows fermentation ends, finally carries out follow-up granulation.
Preferably, described step 2) in yeasting water material mass ratio be 2.5：1.Or, described step 2) in yeasting water material mass ratio be 3.5：2.
Or, described step 2) in yeasting water material mass ratio be 5.5：3.
Preferably, described step 3) in Medium's PH Value be 4.5.
Preferably, described step 4) in water urea content be 2.0%.
Or, described step 4) in water urea content be 3.5%.
Preferably, described step 3) in liquid spawn be prepared as Phanerochaete chrysosporium, geotrichum candidum, aspergillus niger 1:1:1 ratio is inoculated in step 3) described in fluid nutrient medium in carry out shaking table culture produce liquid spawn.
Preferably, described step 6) in inoculum concentration be 20% processing.
Or, described step 6) in inoculum concentration be 35% processing.
Preferably, described step 7) in overthrow when accumulation center temperature reaches 56 DEG C.
Or, described step 7) in accumulation temperature overthrow when reach 60 DEG C.
Embodiment products obtained therefrom is carried out control group test.
Experimental design：
Process one：A green vegetables seed field is using enforcement row gained organic fermentation chemical fertilizer.
Process two：B green vegetables seed field uses control group organic fermentation chemical fertilizer.
Table 2A field and B field contrast test
| Sprout the time (my god) | Sprouting length | Dish leaf density | |
| Experimental group A | 4 | 4.98 | 1605 |
| Control group B | 6 | 3.22 | 1589 |
Experimental result such as upper table 2 is learnt, using this enforcement row institute product green vegetables each side better than control group
Above in association with embodiment, embodiments of the present invention are described in further detail.Above description, the simply specific embodiment of the present invention, various illustrations are not construed as limiting to the flesh and blood of the present invention.
Claims (7)
1. a kind of grape skin produces the method for fertilizer it is characterised in that following steps：
1) grape skin be dried after remove grape pip after and pulverize；
2) by step 1) described in pulverizing Grape Skin be configured to water material mass ratio be 1-8：1-5, regulation humidity is 60-70%, and in heaps；
3) adjusting fluid nutrient medium pH value is 1-7；
4) add water to heap body, and in water, urea content is 1.0-6.0%；
5) preparation of liquid spawn：By Phanerochaete chrysosporium, geotrichum candidum, aspergillus niger 1-5:1-6:The ratio of 1-8 is inoculated in step 3) described in fluid nutrient medium in carry out shaking table culture produce liquid spawn；
6) liquid spawn is inoculated in grape skin by the inoculum concentration of the mass fraction of 10-60%, stirs；
7) mixture is stacked fermentation, and heap body core temperature reaches 30-70 DEG C of turning, adjust humidity simultaneously；
8) in the presence of fungi mixed bacteria, grape skin fully becomes thoroughly decomposed and central temperature is gradually reduced, and shows fermentation ends, finally carries out follow-up granulation.
2. preparation method according to claim 1 is it is characterised in that described step 2) in water material mass ratio be 2.5：1.
3. preparation method according to claim 1 is it is characterised in that step 3) in Medium's PH Value be 4.5.
4. preparation method according to claim 1 is it is characterised in that step 4) in added water urea content be 2.0%.
5. preparation method according to claim 1.It is characterized in that:Described step 5) in liquid spawn be prepared as Phanerochaete chrysosporium, geotrichum candidum, aspergillus niger 1:1:1 ratio is inoculated in step 3) described in fluid nutrient medium in carry out shaking table culture produce liquid spawn.
6. preparation method according to claim 1 it is characterised in that：Described step 6) in inoculum concentration be 20% processing.
7. preparation method according to claim 1 it is characterised in that：Described step 7) in overthrow when accumulation center temperature reaches 56 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610386036.7A CN106396783A (en) | 2016-06-03 | 2016-06-03 | Method for preparing organic fertilizer from grape peel pomace |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610386036.7A CN106396783A (en) | 2016-06-03 | 2016-06-03 | Method for preparing organic fertilizer from grape peel pomace |
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| CN106396783A true CN106396783A (en) | 2017-02-15 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108623344A (en) * | 2018-08-16 | 2018-10-09 | 中粮华夏长城葡萄酒有限公司 | A kind of organic fertilizer and its preparation method and application |
| CN109006353A (en) * | 2018-06-06 | 2018-12-18 | 贵州卡农云服务有限公司 | A kind of macadamia seedling medium and preparation method thereof |
| CN109156138A (en) * | 2018-07-30 | 2019-01-08 | 霍山茗源茶树良种开发有限公司 | A kind of fertilizing method improving high and cold mountain area tealeaves nutritional quality |
| CN109206223A (en) * | 2018-10-18 | 2019-01-15 | 兴义市军创种养殖农民专业合作社 | A kind of fertilizer and preparation method thereof enhancing Cold-resistant Grape performance |
| CN114031431A (en) * | 2021-12-28 | 2022-02-11 | 燕山大学 | Humic acid-rich pig manure compost prepared from wine grape skin residues and method and application thereof |
-
2016
- 2016-06-03 CN CN201610386036.7A patent/CN106396783A/en not_active Withdrawn
Non-Patent Citations (1)
| Title |
|---|
| 刘雪莲: "葡萄皮渣固态发酵生产有机肥的影响因素", 《北方园艺》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006353A (en) * | 2018-06-06 | 2018-12-18 | 贵州卡农云服务有限公司 | A kind of macadamia seedling medium and preparation method thereof |
| CN109156138A (en) * | 2018-07-30 | 2019-01-08 | 霍山茗源茶树良种开发有限公司 | A kind of fertilizing method improving high and cold mountain area tealeaves nutritional quality |
| CN108623344A (en) * | 2018-08-16 | 2018-10-09 | 中粮华夏长城葡萄酒有限公司 | A kind of organic fertilizer and its preparation method and application |
| CN109206223A (en) * | 2018-10-18 | 2019-01-15 | 兴义市军创种养殖农民专业合作社 | A kind of fertilizer and preparation method thereof enhancing Cold-resistant Grape performance |
| CN114031431A (en) * | 2021-12-28 | 2022-02-11 | 燕山大学 | Humic acid-rich pig manure compost prepared from wine grape skin residues and method and application thereof |
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Application publication date: 20170215 |