CN110041117A - A kind of culture medium and preparation method thereof improving capsicum salt tolerance - Google Patents
A kind of culture medium and preparation method thereof improving capsicum salt tolerance Download PDFInfo
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- CN110041117A CN110041117A CN201910166868.1A CN201910166868A CN110041117A CN 110041117 A CN110041117 A CN 110041117A CN 201910166868 A CN201910166868 A CN 201910166868A CN 110041117 A CN110041117 A CN 110041117A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention belongs to agricultural plantings to terminate field, and in particular to a kind of culture medium and preparation method thereof for improving capsicum salt tolerance.The present invention includes following component in parts by weight: cow dung, wormcast, silkworm excrement, bacteria residue, rice bran, wood sawdust, biogas residue, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, complex micro organism fungicide and concentration are the pyrroloquinoline quinone solution of 200-400nmol/L, are prepared by scientific method.Culture medium of the invention includes gas chromatography material, fertilizer and microorganism, pyrroloquinoline quinone solution is added during the preparation process, pepper seedling development and strong sprout can be promoted to be formed, salt tolerance index, photosynthesis characteristics and the enzymatic activity of capsicum are significantly improved after transplanting, illustrate that the salt tolerance of capsicum increases, the ability in saline soil growth on the ground can be reinforced.
Description
Technical field
The invention belongs to agricultural plantings to terminate field, and in particular to it is a kind of improve capsicum salt tolerance culture medium and its preparation
Method.
Background technique
Capsicum is a kind of important solanaceous vegetables, and full of nutrition, contained Vitamin C content is 5 times of tomato, eggplant
20 times, carotene carotene content is 7-15 times of tomato, 20-35 times of eggplant.Capsicum is the maximum vegetables of China's cultivated area
One of crop, China's capsicum total output has occupied first place in the world within 1997,28,000,000 tons of annual output, accounts for about world's yield of hot pepper
46%, pepper planting area and economic value have surmounted cabbage as the first big vegetable crop.Pepper planting process at present
In fertilizer it is unreasonable use etc. due to, pepper planting soil there is salination situation, significantly impact the life of capsicum
It is long.
Summary of the invention
Against the above technical problems, the object of the present invention is to provide a kind of culture medium for improving capsicum salt tolerance and its preparations
Method.
To achieve the goals above, the present invention adopts the following technical scheme:
A kind of culture medium improving capsicum salt tolerance, including following component in parts by weight: 20-40 parts of cow dung, earthworm
20-40 parts of excrement, 10-30 parts of silkworm excrement, 10-20 parts of bacteria residue, 10-20 parts of rice bran, 10-20 parts of wood sawdust, 10-20 parts of biogas residue, nitrogenous fertilizer
5-15 parts, 5-10 parts of phosphate fertilizer, 10-20 parts of potash fertilizer, 5-10 parts of complex micro organism fungicide and concentration are the pyrrole of 200-400nmol/L
Cough up quinoline quinone solution.
Further, the nitrogenous fertilizer is the urea of nitrogen content 46%, and phosphate fertilizer is the calcium superphosphate of phosphorus content 16%, and potash fertilizer is
Alkalescent solubility in citric acid potash fertilizer+potassium content 10% woody peat and draft mud of potassium sulfate+potassium content 24% of potassium content 51%
The mixture of charcoal.
Preferably, the mass ratio of three kinds of potash fertilizer is 2:4:4.
Preferably, the mixing mass ratio of the woody peat and meadow peat is 7:1.
Further, it bacillus laterosporus in the complex micro organism fungicide: Te Jila bacillus: Trichoderma harzianum: rubs
The mould mass ratio of western sacculus is 2:3:1:2.
Preferably, the concentration for the pyrroloquinoline quinone solution applied in the culture medium is 300nmol/L.
A kind of preparation method for the culture medium improving capsicum salt tolerance, comprising the following steps:
(1) crushing and mistake after drying the mixture of bacteria residue, rice bran, wood sawdust, biogas residue and woody peat and meadow peat
100 meshes, obtain comminuting matter；
(2) cow dung, wormcast and silkworm excrement are added in the comminuting matter, adds complex micro organism fungicide and is sent out
Ferment obtains fermentation material；
(3) alkalescent solubility in citric acid potash fertilizer is crushed, crosses 80 meshes, is added in fermentation material together with nitrogenous fertilizer, phosphate fertilizer, mixing is equal
It is even, obtain culture medium semi-finished product；
(4) pyrroloquinoline quinone solution is added in the culture medium semi-finished product to get the culture medium is arrived.
Further, the fermentation temperature in the step (2) is 35-50 DEG C, fermentation time 15-20d.
Further, the water content of culture medium is 60-65% after pyrroloquinoline quinone solution is added in the step (4).
Compared with prior art, the invention has the following beneficial effects:
(1) the pyrroloquinoline quinone solution added in culture medium can promote pepper seedling dry-matter accumulation, leaf development and root
System's growth keeps growth of seedling healthy and strong, and salt tolerance improves.Capsicum can improve internal proline content after being colonized in saliferous soil
With the accumulation for promoting soluble sugar, proline is adjustable the osmotic potential in cell, reduces moisture loss, and stablize the complete of biomembrane
Whole property, the higher structure of Protein requirement reduce damage of the salt stress to biomembrane normal function, guarantee the eubolism of plant
Function；Secondly, proline energy Scavenger of ROS, improves the activity of peroxidase, superoxide dismutase and catalase,
And regulating cell stable state, reduce destruction of the Lipid peroxidation metabolism to biomembrane.Soluble sugar can not only be used for energy reserve, and can mention
High plant cell solute concentration content, conducive to moisture is absorbed from external environment.It also can increase chlorophyll content, improve capsicum
Net Photosynthetic Rate, stomatal conductance and transpiration rate, to improve capsicum salt tolerance.
(2) potassium is the activator of a variety of enzymes in plant, closely related with every metabolic activity, potassium can promote protein and
The synthesis of chlorophyll, and improve the activity of enzyme.Contain in the potash fertilizer of culture medium addition there are three types of Different Potassium Fertilizer, including be easy to absorb
Inorganic potash fertilizer potassium sulfate, fertilizer efficiency slow lasting alkalescent solubility in citric acid potash fertilizer and fertilizer efficiency are abundant woody compared with the slow but content of organic matter
Peat and meadow peat mixture can quickly and muchly meet during chili growth the needs of to potash fertilizer, improve the benefit of potassium
The salt ion in soil is reduced to capsicum to reduce absorption of the plant to sodium ion with rate and the intracorporal potassium content of capsicum
Damage.It wherein is up to 50-80% as humic acid content in the woody peat of one of potash fertilizer, the content of organic matter is up to 60-90%,
And meadow peat is rich in fiber, loose porous, compounding application can form buffer environment to two kinds of peat in the soil in appropriate proportions,
Direct absorption of the capsicum to salt ion is reduced, the salt tolerance of capsicum is improved.
(3) complex micro organism fungicide being added in culture medium can promote seedlings root to develop, and improve capsicum to mineral element
Absorption and metabolism, promote plant in-vivo element balance, and improve capsicum tissue osmotic adjustment ability, promote to moisture and support
The absorption divided, to improve the salt tolerance of capsicum.
(4) when prepared by culture medium that plant organic matter feed powder is broken, add animal wastes and complex micro organism fungicide together
Fermentation can be shortened fermentation time, and can remove the harmful substances such as pathogen, weed seed in fermentation material, be added after fermentation
The water content of pyrroloquinoline quinone solution to culture medium is 60-65%, and the more conducively release of alkalescent solubility in citric acid potash fertilizer promotes capsicum
Absorption of the seedling to organic matter and n-p-k element.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention,
Technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is the present invention one
Divide embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making
Every other embodiment obtained, shall fall within the protection scope of the present invention under the premise of creative work.
Cow dung, bacteria residue, rice bran, wood sawdust and biogas residue used in the following embodiment be agricultural production waste, wormcast,
Silkworm excrement, urea, calcium superphosphate, potassium sulfate, alkalescent solubility in citric acid potash fertilizer, woody peat, meadow peat, bacillus laterosporus, spy
Base draw bacillus, Trichoderma harzianum and Glomus mosseae be it is commercially available, pyrroloquinoline quinone is by the medicine life science of Shanghai
Heart Co., Ltd provides.Experimental field salt content is 0.35%.
Embodiment 1
A kind of culture medium improving capsicum salt tolerance, including following component in parts by weight: 20 parts of cow dung, wormcast 40
Part, is answered at 10 parts of silkworm excrement, 20 parts of bacteria residue, 20 parts of rice bran, 10 parts of wood sawdust, 20 parts of biogas residue, 5 parts of nitrogenous fertilizer, 5 parts of phosphate fertilizer, 10 parts of potash fertilizer
10 parts of microbial bacterial agent and concentration are closed as the pyrroloquinoline quinone solution of 200nmol/L.The nitrogenous fertilizer is the urine of nitrogen content 46%
Element, phosphate fertilizer are the calcium superphosphate of phosphorus content 16%, and potash fertilizer is that the alkalescent Chinese holly of potassium sulfate+potassium content 24% of potassium content 51% is molten
Property the woody peat of the potash fertilizer+potassium content 10% and mixture of meadow peat, the mass ratio of three kinds of potash fertilizer is 2:4:4, wherein wood
The mixing mass ratio of this peat and meadow peat is 7:1.Bacillus laterosporus in the complex micro organism fungicide: Te Jila gemma
Bacillus: Trichoderma harzianum: the mass ratio of Glomus mosseae is 2:3:1:2.
A kind of preparation method for the culture medium improving capsicum salt tolerance, comprising the following steps:
(1) crushing and mistake after drying the mixture of bacteria residue, rice bran, wood sawdust, biogas residue and woody peat and meadow peat
100 meshes, obtain comminuting matter；
(2) cow dung, wormcast and silkworm excrement are added in the comminuting matter, adds complex micro organism fungicide and is sent out
Ferment, fermentation temperature are 35-50 DEG C, and fermentation time 15-20d obtains fermentation material；
(3) alkalescent solubility in citric acid potash fertilizer is crushed, crosses 80 meshes, is added in fermentation material together with nitrogenous fertilizer, phosphate fertilizer, mixing is equal
It is even, obtain culture medium semi-finished product；
(4) water content that pyrroloquinoline quinone solution is added to culture medium in the culture medium semi-finished product is 60-65%, i.e.,
Obtain the culture medium.
Embodiment 2
A kind of culture medium improving capsicum salt tolerance, including following component in parts by weight: 30 parts of cow dung, wormcast 30
Part, 20 parts of silkworm excrement, 15 parts of bacteria residue, 15 parts of rice bran, 15 parts of wood sawdust, 15 parts of biogas residue, 10 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 20 parts of potash fertilizer,
The pyrroloquinoline quinone solution that 8 parts of complex micro organism fungicide and concentration are 300nmol/L.The nitrogenous fertilizer is the urine of nitrogen content 46%
Element, phosphate fertilizer are the calcium superphosphate of phosphorus content 16%, and potash fertilizer is that the alkalescent Chinese holly of potassium sulfate+potassium content 24% of potassium content 51% is molten
Property the woody peat of the potash fertilizer+potassium content 10% and mixture of meadow peat, the mass ratio of three kinds of potash fertilizer is 2:4:4, wherein wood
The mixing mass ratio of this peat and meadow peat is 7:1.Bacillus laterosporus in the complex micro organism fungicide: Te Jila gemma
Bacillus: Trichoderma harzianum: the mass ratio of Glomus mosseae is 2:3:1:2.
A kind of preparation method for the culture medium improving capsicum salt tolerance, comprising the following steps:
(1) crushing and mistake after drying the mixture of bacteria residue, rice bran, wood sawdust, biogas residue and woody peat and meadow peat
100 meshes, obtain comminuting matter；
(2) cow dung, wormcast and silkworm excrement are added in the comminuting matter, adds complex micro organism fungicide and is sent out
Ferment, fermentation temperature are 35-50 DEG C, and fermentation time 15-20d obtains fermentation material；
(3) alkalescent solubility in citric acid potash fertilizer is crushed, crosses 80 meshes, is added in fermentation material together with nitrogenous fertilizer, phosphate fertilizer, mixing is equal
It is even, obtain culture medium semi-finished product；
(4) water content that pyrroloquinoline quinone solution is added to culture medium in the culture medium semi-finished product is 60-65%, i.e.,
Obtain the culture medium.
Embodiment 3
A kind of culture medium improving capsicum salt tolerance, including following component in parts by weight: 40 parts of cow dung, wormcast 20
Part, 30 parts of silkworm excrement, 10 parts of bacteria residue, 10 parts of rice bran, 20 parts of wood sawdust, 10 parts of biogas residue, 15 parts of nitrogenous fertilizer, 10 parts of phosphate fertilizer, 15 parts of potash fertilizer,
The pyrroloquinoline quinone solution that 5 parts of complex micro organism fungicide and concentration are 400nmol/L.The nitrogenous fertilizer is the urine of nitrogen content 46%
Element, phosphate fertilizer are the calcium superphosphate of phosphorus content 16%, and potash fertilizer is that the alkalescent Chinese holly of potassium sulfate+potassium content 24% of potassium content 51% is molten
Property the woody peat of the potash fertilizer+potassium content 10% and mixture of meadow peat, the mass ratio of three kinds of potash fertilizer is 2:4:4, wherein wood
The mixing mass ratio of this peat and meadow peat is 7:1.Bacillus laterosporus in the complex micro organism fungicide: Te Jila gemma
Bacillus: Trichoderma harzianum: the mass ratio of Glomus mosseae is 2:3:1:2.
A kind of preparation method for the culture medium improving capsicum salt tolerance, comprising the following steps:
(1) crushing and mistake after drying the mixture of bacteria residue, rice bran, wood sawdust, biogas residue and woody peat and meadow peat
100 meshes, obtain comminuting matter；
(2) cow dung, wormcast and silkworm excrement are added in the comminuting matter, adds complex micro organism fungicide and is sent out
Ferment, fermentation temperature are 35-50 DEG C, and fermentation time 15-20d obtains fermentation material；
(3) alkalescent solubility in citric acid potash fertilizer is crushed, crosses 80 meshes, is added in fermentation material together with nitrogenous fertilizer, phosphate fertilizer, mixing is equal
It is even, obtain culture medium semi-finished product；
(4) water content that pyrroloquinoline quinone solution is added to culture medium in the culture medium semi-finished product is 60-65%, i.e.,
Obtain the culture medium.
Embodiment 4
A kind of culture medium improving capsicum salt tolerance, including following component in parts by weight: 20 parts of cow dung, wormcast 40
Part, 20 parts of silkworm excrement, 10 parts of bacteria residue, 20 parts of rice bran, 15 parts of wood sawdust, 10 parts of biogas residue, 10 parts of nitrogenous fertilizer, 10 parts of phosphate fertilizer, 15 parts of potash fertilizer,
The pyrroloquinoline quinone solution that 10 parts of complex micro organism fungicide and concentration are 200nmol/L.The nitrogenous fertilizer is nitrogen content 46%
Urea, phosphate fertilizer are the calcium superphosphate of phosphorus content 16%, and potash fertilizer is the alkalescent Chinese holly of potassium sulfate+potassium content 24% of potassium content 51%
Dissolubility potash fertilizer+woody peat of potassium content 10% and the mixture of meadow peat, the mass ratio of three kinds of potash fertilizer are 2:4:4, wherein
The mixing mass ratio of woody peat and meadow peat is 7:1.Bacillus laterosporus in the complex micro organism fungicide: Te Jila bud
Spore bacillus: Trichoderma harzianum: the mass ratio of Glomus mosseae is 2:3:1:2.
A kind of preparation method for the culture medium improving capsicum salt tolerance, comprising the following steps:
(1) crushing and mistake after drying the mixture of bacteria residue, rice bran, wood sawdust, biogas residue and woody peat and meadow peat
100 meshes, obtain comminuting matter；
(2) cow dung, wormcast and silkworm excrement are added in the comminuting matter, adds complex micro organism fungicide and is sent out
Ferment, fermentation temperature are 35-50 DEG C, and fermentation time 15-20d obtains fermentation material；
(3) alkalescent solubility in citric acid potash fertilizer is crushed, crosses 80 meshes, is added in fermentation material together with nitrogenous fertilizer, phosphate fertilizer, mixing is equal
It is even, obtain culture medium semi-finished product；
(4) water content that pyrroloquinoline quinone solution is added to culture medium in the culture medium semi-finished product is 60-65%, i.e.,
Obtain the culture medium.
Embodiment 5
A kind of culture medium improving capsicum salt tolerance, including following component in parts by weight: 30 parts of cow dung, wormcast 30
Part, is answered at 30 parts of silkworm excrement, 15 parts of bacteria residue, 15 parts of rice bran, 20 parts of wood sawdust, 15 parts of biogas residue, 5 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 10 parts of potash fertilizer
8 parts of microbial bacterial agent and concentration are closed as the pyrroloquinoline quinone solution of 300nmol/L.The nitrogenous fertilizer is the urine of nitrogen content 46%
Element, phosphate fertilizer are the calcium superphosphate of phosphorus content 16%, and potash fertilizer is that the alkalescent Chinese holly of potassium sulfate+potassium content 24% of potassium content 51% is molten
Property the woody peat of the potash fertilizer+potassium content 10% and mixture of meadow peat, the mass ratio of three kinds of potash fertilizer is 2:4:4, wherein wood
The mixing mass ratio of this peat and meadow peat is 7:1.Bacillus laterosporus in the complex micro organism fungicide: Te Jila gemma
Bacillus: Trichoderma harzianum: the mass ratio of Glomus mosseae is 2:3:1:2.
A kind of preparation method for the culture medium improving capsicum salt tolerance, comprising the following steps:
(1) crushing and mistake after drying the mixture of bacteria residue, rice bran, wood sawdust, biogas residue and woody peat and meadow peat
100 meshes, obtain comminuting matter；
(2) cow dung, wormcast and silkworm excrement are added in the comminuting matter, adds complex micro organism fungicide and is sent out
Ferment, fermentation temperature are 35-50 DEG C, and fermentation time 15-20d obtains fermentation material；
(3) alkalescent solubility in citric acid potash fertilizer is crushed, crosses 80 meshes, is added in fermentation material together with nitrogenous fertilizer, phosphate fertilizer, mixing is equal
It is even, obtain culture medium semi-finished product；
(4) water content that pyrroloquinoline quinone solution is added to culture medium in the culture medium semi-finished product is 60-65%, i.e.,
Obtain the culture medium.
Embodiment 6
A kind of culture medium improving capsicum salt tolerance, including following component in parts by weight: 40 parts of cow dung, wormcast 20
Part, 10 parts of silkworm excrement, 20 parts of bacteria residue, 10 parts of rice bran, 10 parts of wood sawdust, 20 parts of biogas residue, 15 parts of nitrogenous fertilizer, 5 parts of phosphate fertilizer, 20 parts of potash fertilizer,
The pyrroloquinoline quinone solution that 5 parts of complex micro organism fungicide and concentration are 400nmol/L.The nitrogenous fertilizer is the urine of nitrogen content 46%
Element, phosphate fertilizer are the calcium superphosphate of phosphorus content 16%, and potash fertilizer is that the alkalescent Chinese holly of potassium sulfate+potassium content 24% of potassium content 51% is molten
Property the woody peat of the potash fertilizer+potassium content 10% and mixture of meadow peat, the mass ratio of three kinds of potash fertilizer is 2:4:4, wherein wood
The mixing mass ratio of this peat and meadow peat is 7:1.Bacillus laterosporus in the complex micro organism fungicide: Te Jila gemma
Bacillus: Trichoderma harzianum: the mass ratio of Glomus mosseae is 2:3:1:2.
A kind of preparation method for the culture medium improving capsicum salt tolerance, comprising the following steps:
(1) crushing and mistake after drying the mixture of bacteria residue, rice bran, wood sawdust, biogas residue and woody peat and meadow peat
100 meshes, obtain comminuting matter；
(2) cow dung, wormcast and silkworm excrement are added in the comminuting matter, adds complex micro organism fungicide and is sent out
Ferment, fermentation temperature are 35-50 DEG C, and fermentation time 15-20d obtains fermentation material；
(3) alkalescent solubility in citric acid potash fertilizer is crushed, crosses 80 meshes, is added in fermentation material together with nitrogenous fertilizer, phosphate fertilizer, mixing is equal
It is even, obtain culture medium semi-finished product；
(4) water content that pyrroloquinoline quinone solution is added to culture medium in the culture medium semi-finished product is 60-65%, i.e.,
Obtain the culture medium.
Embodiment 7
A kind of culture medium improving capsicum salt tolerance, including following component in parts by weight: 20 parts of cow dung, wormcast 40
Part, 30 parts of silkworm excrement, 15 parts of bacteria residue, 20 parts of rice bran, 10 parts of wood sawdust, 15 parts of biogas residue, 15 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 20 parts of potash fertilizer,
The pyrroloquinoline quinone solution that 10 parts of complex micro organism fungicide and concentration are 200nmol/L.The nitrogenous fertilizer is nitrogen content 46%
Urea, phosphate fertilizer are the calcium superphosphate of phosphorus content 16%, and potash fertilizer is the alkalescent Chinese holly of potassium sulfate+potassium content 24% of potassium content 51%
Dissolubility potash fertilizer+woody peat of potassium content 10% and the mixture of meadow peat, the mass ratio of three kinds of potash fertilizer are 2:4:4, wherein
The mixing mass ratio of woody peat and meadow peat is 7:1.Bacillus laterosporus in the complex micro organism fungicide: Te Jila bud
Spore bacillus: Trichoderma harzianum: the mass ratio of Glomus mosseae is 2:3:1:2.
A kind of preparation method for the culture medium improving capsicum salt tolerance, comprising the following steps:
(1) crushing and mistake after drying the mixture of bacteria residue, rice bran, wood sawdust, biogas residue and woody peat and meadow peat
100 meshes, obtain comminuting matter；
(2) cow dung, wormcast and silkworm excrement are added in the comminuting matter, adds complex micro organism fungicide and is sent out
Ferment, fermentation temperature are 35-50 DEG C, and fermentation time 15-20d obtains fermentation material；
(3) alkalescent solubility in citric acid potash fertilizer is crushed, crosses 80 meshes, is added in fermentation material together with nitrogenous fertilizer, phosphate fertilizer, mixing is equal
It is even, obtain culture medium semi-finished product；
(4) water content that pyrroloquinoline quinone solution is added to culture medium in the culture medium semi-finished product is 60-65%, i.e.,
Obtain the culture medium.
Embodiment 8
A kind of culture medium improving capsicum salt tolerance, including following component in parts by weight: 30 parts of cow dung, wormcast 30
Part, 10 parts of silkworm excrement, 10 parts of bacteria residue, 15 parts of rice bran, 20 parts of wood sawdust, 10 parts of biogas residue, 5 parts of nitrogenous fertilizer, 10 parts of phosphate fertilizer, 15 parts of potash fertilizer,
The pyrroloquinoline quinone solution that 8 parts of complex micro organism fungicide and concentration are 300nmol/L.The nitrogenous fertilizer is the urine of nitrogen content 46%
Element, phosphate fertilizer are the calcium superphosphate of phosphorus content 16%, and potash fertilizer is that the alkalescent Chinese holly of potassium sulfate+potassium content 24% of potassium content 51% is molten
Property the woody peat of the potash fertilizer+potassium content 10% and mixture of meadow peat, the mass ratio of three kinds of potash fertilizer is 2:4:4, wherein wood
The mixing mass ratio of this peat and meadow peat is 7:1.Bacillus laterosporus in the complex micro organism fungicide: Te Jila gemma
Bacillus: Trichoderma harzianum: the mass ratio of Glomus mosseae is 2:3:1:2.
A kind of preparation method for the culture medium improving capsicum salt tolerance, comprising the following steps:
(1) crushing and mistake after drying the mixture of bacteria residue, rice bran, wood sawdust, biogas residue and woody peat and meadow peat
100 meshes, obtain comminuting matter；
(2) cow dung, wormcast and silkworm excrement are added in the comminuting matter, adds complex micro organism fungicide and is sent out
Ferment, fermentation temperature are 35-50 DEG C, and fermentation time 15-20d obtains fermentation material；
(3) alkalescent solubility in citric acid potash fertilizer is crushed, crosses 80 meshes, is added in fermentation material together with nitrogenous fertilizer, phosphate fertilizer, mixing is equal
It is even, obtain culture medium semi-finished product；
(4) water content that pyrroloquinoline quinone solution is added to culture medium in the culture medium semi-finished product is 60-65%, i.e.,
Obtain the culture medium.
Embodiment 9
A kind of culture medium improving capsicum salt tolerance, including following component in parts by weight: 40 parts of cow dung, wormcast 20
Part, 20 parts of silkworm excrement, 20 parts of bacteria residue, 10 parts of rice bran, 15 parts of wood sawdust, 20 parts of biogas residue, 10 parts of nitrogenous fertilizer, 5 parts of phosphate fertilizer, 10 parts of potash fertilizer,
The pyrroloquinoline quinone solution that 5 parts of complex micro organism fungicide and concentration are 400nmol/L.The nitrogenous fertilizer is the urine of nitrogen content 46%
Element, phosphate fertilizer are the calcium superphosphate of phosphorus content 16%, and potash fertilizer is that the alkalescent Chinese holly of potassium sulfate+potassium content 24% of potassium content 51% is molten
Property the woody peat of the potash fertilizer+potassium content 10% and mixture of meadow peat, the mass ratio of three kinds of potash fertilizer is 2:4:4, wherein wood
The mixing mass ratio of this peat and meadow peat is 7:1.Bacillus laterosporus in the complex micro organism fungicide: Te Jila gemma
Bacillus: Trichoderma harzianum: the mass ratio of Glomus mosseae is 2:3:1:2.
A kind of preparation method for the culture medium improving capsicum salt tolerance, comprising the following steps:
(1) crushing and mistake after drying the mixture of bacteria residue, rice bran, wood sawdust, biogas residue and woody peat and meadow peat
100 meshes, obtain comminuting matter；
(2) cow dung, wormcast and silkworm excrement are added in the comminuting matter, adds complex micro organism fungicide and is sent out
Ferment, fermentation temperature are 35-50 DEG C, and fermentation time 15-20d obtains fermentation material；
(3) alkalescent solubility in citric acid potash fertilizer is crushed, crosses 80 meshes, is added in fermentation material together with nitrogenous fertilizer, phosphate fertilizer, mixing is equal
It is even, obtain culture medium semi-finished product；
(4) water content that pyrroloquinoline quinone solution is added to culture medium in the culture medium semi-finished product is 60-65%, i.e.,
Obtain the culture medium.
Comparative example 1 is the capsicum that conventional medium is cultivated.
Comparative example 2 is not the difference from embodiment 1 is that pyrroloquinoline quinone solution is added in culture medium.
Comparative example 3 is the difference from embodiment 1 is that the potash fertilizer in culture medium is conventional potassium sulfate.
Comparative example 4 is not the difference from embodiment 1 is that complex micro organism fungicide is added in culture medium.
Pepper seedling botanic conformation under 1 different disposal of table
From the data in table 1, it can be seen that being cultivated compared with the pepper seedling that the conventional medium of comparative example 1 is cultivated using the present invention
Plant height, stem thickness, the number of blade, root long, overground part dry weight and the complete stool dry weight of the embodiment 1-9 pepper seedling of base have obvious increasing
Add, seedling growth is more healthy and stronger, and salt tolerance is higher after transplanting.It is compared by embodiment 1-9 it is found that embodiment 2,5,8 promotes capsicum children
The effect of seedling growth is more preferable, illustrates that the most suitable of pyrroloquinoline quinone solution uses concentration for 300nmol/L, is secondly 400nmol/L,
It is finally 200nmol/L, embodiment 5 known to comprehensive analysis is preferred plan.
By comparative example 2-4 data as known from compared to Example 1, the comparison of pyrroloquinoline quinone solution is not added in culture medium
The influence that example 2 grows pepper seedling is maximum, and pepper seedling is more thin and weak.Pair of conventional sulfuric acid potassium is used only in potash fertilizer in culture medium
Ratio 3 and pepper seedling growing state is not added in the comparative example 4 of complex micro organism fungicide without significant difference, it is peppery under two processing
Green pepper growth of seedling situation is better than comparative example 1, but still poor compared with Example 1.
2 capsicum proline of table, chlorophyll, malonaldehyde, soluble sugar and soluble protein content variation
From the data in table 2, it can be seen that being cultivated compared with the pepper seedling that the conventional medium of comparative example 1 is cultivated using the present invention
Proline, chlorophyll, soluble sugar and the soluble protein content of capsicum are significantly increased in the embodiment 1-9 of base, malonaldehyde
Content is substantially reduced, and the capsicum salt tolerance for illustrating that the present invention cultivates is higher.It is compared by embodiment 1-9 it is found that in embodiment 2,5,8
Capsicum salt tolerance index is more preferable, illustrates that the most suitable of pyrroloquinoline quinone solution uses concentration for 300nmol/L, is secondly 400nmol/
L is finally 200nmol/L, and embodiment 5 known to comprehensive analysis is preferred plan.
By comparative example 2-4 data as known from compared to Example 1, the comparison of pyrroloquinoline quinone solution is not added in culture medium
2 capsicum salt tolerance index of example is poor.Potash fertilizer in culture medium is used only the comparative example 3 of conventional sulfuric acid potassium and composite microbial is not added
Capsicum salt tolerance index is higher than comparative example 1 without significant difference, two treatment effects in the comparative example 4 of object microbial inoculum, but lower than implementation
Example 1.
The variation of table 3 Photosynthetic Character of Pepper and enzymatic activity
From the data in table 3, it can be seen that being cultivated compared with the pepper seedling that the conventional medium of comparative example 1 is cultivated using the present invention
The Net Photosynthetic Rate of capsicum, stomatal conductance and transpiration rate improve in the embodiment 1-9 of base, and photosynthesis characteristics are enhanced, super oxygen
Compound mutase, peroxidase and catalase activity are improved, and the physiological activity of capsicum is more vigorous, illustrate the present invention
The capsicum salt tolerance of cultivation is higher, and the growth of capsicum is more preferable.Compared by embodiment 1-9 it is found that in embodiment 2,5,8 capsicum light
It closes characteristic and transpiration is more vigorous, the activity of three kinds of enzymes is higher, illustrates that the most suitable of pyrroloquinoline quinone solution is using concentration
Secondly 300nmol/L is 400nmol/L, is finally 200nmol/L, embodiment 5 known to comprehensive analysis is preferred plan.
By comparative example 2-4 data as known from compared to Example 1, the comparison of pyrroloquinoline quinone solution is not added in culture medium
2 Photosynthetic Character of Pepper of example and transpiration are poor, and enzymatic activity is lower.The comparison of conventional sulfuric acid potassium is used only in potash fertilizer in culture medium
Example 3 and Photosynthetic Character of Pepper, transpiration and enzymatic activity are not added in the comparative example 4 of complex micro organism fungicide without significant difference,
Two treatment effects are higher than comparative example 1, but are lower than embodiment 1.
Claims (9)
1. a kind of culture medium for improving capsicum salt tolerance, which is characterized in that including following component in parts by weight: cow dung 20-
40 parts, 20-40 parts of wormcast, 10-30 parts of silkworm excrement, 10-20 parts of bacteria residue, 10-20 parts of rice bran, 10-20 parts of wood sawdust, biogas residue 10-
20 parts, 5-15 parts of nitrogenous fertilizer, 5-10 parts of phosphate fertilizer, 10-20 parts of potash fertilizer, 5-10 parts of complex micro organism fungicide and concentration are 200-
The pyrroloquinoline quinone solution of 400nmol/L.
2. a kind of culture medium for improving capsicum salt tolerance according to claim 1, which is characterized in that the nitrogenous fertilizer is nitrogenous
The urea of amount 46%, phosphate fertilizer are the calcium superphosphate of phosphorus content 16%, and potash fertilizer is potassium sulfate+potassium content 24% of potassium content 51%
Alkalescent solubility in citric acid potash fertilizer+woody peat of potassium content 10% and the mixture of meadow peat.
3. a kind of culture medium for improving capsicum salt tolerance according to claim 2, which is characterized in that three kinds of potash fertilizer
Mass ratio is 2:4:4.
4. it is according to claim 2 it is a kind of improve capsicum salt tolerance culture medium, which is characterized in that the woody peat and
The mixing mass ratio of meadow peat is 7:1.
5. a kind of culture medium for improving capsicum salt tolerance according to claim 1, which is characterized in that the complex microorganism
Bacillus laterosporus in microbial inoculum: Te Jila bacillus: Trichoderma harzianum: the mass ratio of Glomus mosseae is 2:3:1:2.
6. a kind of culture medium for improving capsicum salt tolerance according to claim 1, which is characterized in that applied in the culture medium
The concentration of pyrroloquinoline quinone solution is 300nmol/L.
7. a kind of preparation method of culture medium for improving capsicum salt tolerance according to claim 1-6, feature
It is, comprising the following steps:
(1) it is crushed after drying the mixture of bacteria residue, rice bran, wood sawdust, biogas residue and woody peat and meadow peat and crosses 100 mesh
Sieve, obtains comminuting matter；
(2) cow dung, wormcast and silkworm excrement are added in the comminuting matter, adds complex micro organism fungicide and ferments, obtain
To fermentation material；
(3) alkalescent solubility in citric acid potash fertilizer is crushed, crosses 80 meshes, is added in fermentation material together with nitrogenous fertilizer, phosphate fertilizer, be uniformly mixed,
Obtain culture medium semi-finished product；
(4) pyrroloquinoline quinone solution is added in the culture medium semi-finished product to get the culture medium is arrived.
8. a kind of preparation method of culture medium for improving capsicum salt tolerance according to claim 7, which is characterized in that described
Fermentation temperature in step (2) is 35-50 DEG C, fermentation time 15-20d.
9. a kind of preparation method of culture medium for improving capsicum salt tolerance according to claim 7, which is characterized in that described
The water content of culture medium is 60-65% after pyrroloquinoline quinone solution is added in step (4).
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