CN104498363A - Growth promoting endophytic beauveria bassiana strain and application thereof - Google Patents
Growth promoting endophytic beauveria bassiana strain and application thereof Download PDFInfo
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- CN104498363A CN104498363A CN201410437440.3A CN201410437440A CN104498363A CN 104498363 A CN104498363 A CN 104498363A CN 201410437440 A CN201410437440 A CN 201410437440A CN 104498363 A CN104498363 A CN 104498363A
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Abstract
The invention discloses a growth promoting endophytic beauveria bassiana strain and application thereof. The endophytic beauveria bassiana strain is Beauveria bassiana Bb4, is registered and preserved in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms on April 28, 2014, and the preservation number is CGMCC No.9097. The strain is inoculated to maize, wheat and the like, can colonize in a host body and promote the growth of the host plant, and has certain toxicity to corn borer larvae. Application of the strain to production of microbial fertilizer preparations is conducive to reducing pesticide residues and protecting the ecological environment.
Description
Technical field
The present invention relates to a kind of somatotrophic plant endogenesis Strain of Beauveria bassiana and application.
Background technology
Endogenetic fungus is exerted one's influence to plant by means of signal function by self meta-bolites or existence itself, thus plays an important role in the complex ecosystem of " endophyte--plant--animal--environment ".A lot of research shows, the host having infected endogenetic fungus often has the advantages such as growth quick, degeneration-resistant border, disease resistance, anti-animal injury, has more struggle for existence power than non-infected plant; And because endogenetic fungus distribution is wide, kind is many, thus plant endogenesis epiphyte has caused the broad interest of microbiologist, ecologist, plant protection scholar in recent years, becomes the focus of research both at home and abroad gradually; Current discovery, endophyte has the beneficial effect of the following aspects at least to host plant: promote the growing of host plant, strengthen drought-resistant, the high temperature resistance patience of host plant, anti-animal (insect, nematode, food grass Mammals etc.) ingests, disease-resistant fungal pathogens and bacterium and allelopathy etc.
A lot of plant endogenesis epiphytes has growth promoting function, and Diene etc. find endogenetic fungus
helminthosporium velutinumthe biomass of sweet sorghum obviously can be increased after infecting host; Malinowski etc. find that the fescue root system having infected endogenetic fungus has the ability of stronger absorption P, and in root system, Ca, Mg content is also higher simultaneously; Research discovery 3 kinds of Mycena endogenetic fungus such as height is slight can promote Herba Dendrobii growth of seedling, promote the sprouting of Herba Anoectochili roxburghii lateral bud and side root to have actual application value to the successful cultivation of the iron sheet stone stem of noble dendrobium and Herba Anoectochili roxburghii.
Beauveria bassiana is a kind of insect pathogenic fungus, is distributed widely in the whole world, can parasitic Coleoptera, Hemiptera, lepidopteran etc. totally 15 orders, 149 sections, 521 belong to, 6 sections, more than 10 of 707 kinds of insects and acarina plant mite and slave girl.Have virulence strong, the advantages such as host range is wide, to people, animal toxicological harmless, does not injure natural enemy, free from environmental pollution, are widely used in the integrated control of harmful organism.
Beauveria bassiana is not only a kind of insect pathogenic fungus, is also the endogenetic fungus of plant.From eighties of last century nineties, domestic scholars is separated to beauveria bassiana successively in the plant materialss such as tomato, potato, cotton, coffee, banana.But still do not see the report that interior green-ball beauveria bassiana promotes host plant growth at present.The statistical figure issued according to State Statistics Bureau show, within 2012, corn has become the first food crop kind of China, if interior green-ball beauveria bassiana has growgh promoting effects, provides reference frame by the high yield high yield for corn.
Summary of the invention
The object of the present invention is to provide a kind of somatotrophic plant endogenesis Strain of Beauveria bassiana and application.
Technical scheme of the present invention is as follows:
Somatotrophic plant endogenesis Strain of Beauveria bassiana, described plant endogenesis Strain of Beauveria bassiana is Strain of Beauveria bassiana (Beauveria bassiana) Bb4, register preservation on April 28th, 2014 at China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.9097, and depositary institution address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
Somatotrophic plant endogenesis Strain of Beauveria bassiana, described Strain of Beauveria bassiana (
beauveria bassiana) the bacterium colony powdery of Bb4, in white, gradually become faint yellow after a while; Described bacterial strain mycelia has barrier film and branch; Conidium is subsphaeroidal or spherical, transparent, smooth; Conidiogenous cell bottle stalk shape, is born on mycelia, conidiophore or stalk cell, conidiophore or the vesicle that expands can be formed and produce spore axle, in geniculation, namely in a zigzag.
The plant endogenesis beauveria bassiana bacterial classification of preservation is pressed 5% inoculum size in SDAY solid medium, activation is cultivated can prepare spore suspension in 10-14 days.
The plant endogenesis beauveria bassiana bacterial classification of preservation is pressed 2-3% inoculum size in liquid SDAY substratum, activation is cultivated and is obtained bacterium liquid in 7-15 days.
The described application of somatotrophic plant endogenesis Strain of Beauveria bassiana in the seed or seedling of the crop such as corn, wheat.
With fungi universal primer ITS1/ITS4, this bacterial strain is carried out to the pcr amplification of rDNA ITS, and ITS pcr amplification product is checked order, complete nucleotide sequence is spliced into after sequencing result being removed vector primer sequence, its size is 569 bp, gained sequence is carried out blast search in GenBank GenBank, finds the ITS region of test strain and the beauveria bassiana reported
b. bassianaand teleomorph
codyceps bassianahomology the highest, majority reaches 100%; Utilize software analysis, adopt adjacent method construct evolutionary tree, in conjunction with its morphological feature, therefore determine this bacterial strain be beauveria bassiana (
b. bassiana).
Plant endogenesis epiphyte of the present invention can be cultivated by the microbial solid fermentation of routine or liquid fermentation process, and obtained with plant endogenesis epiphyte of the present invention----Nei green-ball beauveria bassiana is the microbial fertilizer of main component; The optimum growth temperature of this bacterium is 25 DEG C, optimal pH is 6.0-7.0, in available general fermentation industry, solid-state fermentation tank and mechanical agitating fermentation tank are cultivated, manufacture with interior green-ball beauveria bassiana of the present invention for microbial fertilizer time, the substratum that in this technical field, known beauveria bassiana biocontrol fungicide is general can be used, be mixed with the formulation such as aqua, pulvis.
Beneficial effect of the present invention:
Strain inoculation of the present invention, in corn, wheat etc., at host's Colonization inside plants, can promote the growth of host plant, have certain toxicity to corn borer larvae; By this bacterial strain for the production of microbial fertilizer preparation, contribute to reducing pesticide residue, preserve the ecological environment.
Accompanying drawing explanation
In Fig. 1, after green-ball beauveria bassiana method for soaking seed process 20d, corn Different Organs internal object bacterium reclaims result;
In Fig. 2, green-ball beauveria bassiana is on the impact of corn germination;
In Fig. 3, green-ball beauveria bassiana is on the impact of corn emergence rate;
In Fig. 4, green-ball beauveria bassiana is on the impact of maize seedling roots vigor;
In Fig. 5, green-ball beauveria bassiana is on the impact of corn seedling chlorophyll content.
Embodiment
Embodiment 1: interior green-ball beauveria bassiana determines the situation of growing by method for soaking seed in Corn, as shown in Figure 1:
Select and produce each bacterial strain flat board of the good beauveria bassiana of spore, add in 50ml sterilized water with transfering loop scraping spore, after vortex oscillation instrument vibration mixing, filter, counting, adjusting spore suspension concentration is 5 × 10
7individual/ml, selects the corn seed of full grains, puts into spore suspension and soaks 8h, by under the corn seed kind of immersion treatment, carries out mark, contrast sterilized water process.Not only be separated in root after 20d and obtain inoculating bacterium, can be separated equally in stem, leaf, petiole and obtain inoculating bacterium, illustrates that thalline can by the seed coat of corn invasion plant, and thalline can from root to stem, leaf portion shifts.
Embodiment 2: interior green-ball beauveria bassiana urgees corn germination experiment, as shown in Figure 2:
Select and produce each bacterial strain flat board of the good beauveria bassiana of spore, preparation concentration is 1 × 10
6the spore suspension of individual/ml; To plant grain respectively full, corn seed of uniform size immerses in 75% ethanol and soaks 10min, in immersing in green-ball beauveria bassiana spore suspension, takes out after soaking 12h under room temperature after aseptic water washing, with the seed soaked with sterilized water for contrast.
Blot unnecessary suspension with sterilized thieving paper, be placed in the culture dish being covered with and soaking filter paper with sterilized water, 20, every ware, each process 5 wares, repeat, are placed in 25 DEG C of illumination boxs and cultivate 3d for 5 times, record number of shoots every 12h, calculate germinating energy and percentage of germination.Result shows, and after using interior green-ball beauveria bassiana seed soaking corn, can significantly improve the germinating energy of corn, percentage of germination.
Embodiment 3: interior green-ball beauveria bassiana urgees growth of maize experiment;
Random choose corn seed, after surface sterilization, is 1 × 10 by concentration
7the beauveria bassiana spore suspension of individual/ml is planted in sterilizing vermiculite after soaking 12h, and 25 DEG C of greenhouse experiments, water appropriate amounts of sterilized water, and 30d " Invest, Then Investigate " is emerged number, plant height, biomass and improving activity of root system, and each process 100 strain, establishes 5 repetitions altogether.With the seed of sterilized water immersion treatment in contrast.Green-ball beauveria bassiana seedling rate, biomass, plant height, improving activity of root system, plant leaf Chlorophyll content in result display, have certain growth promoting function to corn, as shown in table 1, Fig. 3,4,5.
In table 1, green-ball beauveria bassiana is on the impact of corn seedling biomass and plant height
Claims (5)
1. a somatotrophic plant endogenesis Strain of Beauveria bassiana, it is characterized in that, described plant endogenesis Strain of Beauveria bassiana is Strain of Beauveria bassiana (Beauveria bassiana) Bb4, register preservation on April 28th, 2014 at China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.9097.
2. somatotrophic plant endogenesis Strain of Beauveria bassiana according to claim 1, is characterized in that, the bacterium colony powdery of described Strain of Beauveria bassiana (Beauveria bassiana) Bb4, in white, gradually becomes faint yellow after a while; Described bacterial strain mycelia has barrier film and branch; Conidium is subsphaeroidal or spherical, transparent, smooth; Conidiogenous cell bottle stalk shape, is born on mycelia, conidiophore or stalk cell, conidiophore or the vesicle that expands can be formed and produce spore axle, in geniculation, namely in a zigzag.
3. the method for cultivation of a somatotrophic plant endogenesis Strain of Beauveria bassiana as claimed in claim 1, it is characterized in that, the plant endogenesis beauveria bassiana bacterial classification of preservation is pressed 5% inoculum size in SDAY solid medium, activation is cultivated can prepare spore suspension in 10-14 days.
4. the method for cultivation of a somatotrophic plant endogenesis Strain of Beauveria bassiana as claimed in claim 1, it is characterized in that, the plant endogenesis beauveria bassiana bacterial classification of preservation is pressed 2-3% inoculum size in liquid SDAY substratum, activation is cultivated and is obtained bacterium liquid in 7-15 days.
5. the application of somatotrophic plant endogenesis Strain of Beauveria bassiana as claimed in claim 1 in the seed or seedling of the crop such as corn, wheat.
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| Application Number | Priority Date | Filing Date | Title |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106717306A (en) * | 2016-11-29 | 2017-05-31 | 吉林省农业科学院 | Beauveria bassiana colonizes method and its application Maize at Seedling Stage |
| CN106818886A (en) * | 2016-12-25 | 2017-06-13 | 吴迪 | A kind of preparation method and applications of double-deck dampproof protection against insects wheat seed coating agent |
| CN107090411A (en) * | 2017-05-04 | 2017-08-25 | 皮玉婷 | The beauveria bassiana HAr19 bacterial strains of one plant of degradable alkyl phenol and its application |
| CN112375688A (en) * | 2020-07-27 | 2021-02-19 | 吉林省农业科学院 | Beauveria bassiana and application thereof in biological prevention and control of corn northern leaf blight |
| CN113373067A (en) * | 2021-07-28 | 2021-09-10 | 贵州省烟草公司遵义市公司 | Compound microbial agent capable of promoting plant growth and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106717306A (en) * | 2016-11-29 | 2017-05-31 | 吉林省农业科学院 | Beauveria bassiana colonizes method and its application Maize at Seedling Stage |
| CN106818886A (en) * | 2016-12-25 | 2017-06-13 | 吴迪 | A kind of preparation method and applications of double-deck dampproof protection against insects wheat seed coating agent |
| CN107090411A (en) * | 2017-05-04 | 2017-08-25 | 皮玉婷 | The beauveria bassiana HAr19 bacterial strains of one plant of degradable alkyl phenol and its application |
| CN107090411B (en) * | 2017-05-04 | 2020-09-25 | 广州禾立田生物科技有限公司 | Beauveria bassiana HAr19 strain capable of degrading alkylphenol and application thereof |
| CN112375688A (en) * | 2020-07-27 | 2021-02-19 | 吉林省农业科学院 | Beauveria bassiana and application thereof in biological prevention and control of corn northern leaf blight |
| CN113373067A (en) * | 2021-07-28 | 2021-09-10 | 贵州省烟草公司遵义市公司 | Compound microbial agent capable of promoting plant growth and application thereof |
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