CN106818886A - A kind of preparation method and applications of double-deck dampproof protection against insects wheat seed coating agent - Google Patents
A kind of preparation method and applications of double-deck dampproof protection against insects wheat seed coating agent Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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Abstract
The invention discloses a kind of preparation method and applications of double-deck dampproof protection against insects wheat seed coating agent, belong to agricultural materials preparing technical field.The present invention is first cultivated, collection obtains beauveria bassiana robe powder, added in the transparent liquid that sodium carboxymethylcellulose and gelatin are prepared, it is well mixed to obtain component A, then use diatomite, with neopelex after the crushing of the materials such as talcum powder, polyethylene glycol mixes, obtain component B, component A is coated on wheat seed surface again, component B is sprayed after not being completely dried, the double-deck wheat seed for planting clothing film must be carried after drying, the present invention is using the addition of microbial insecticide beauveria bassiana robe powder in film forming agent, it is coated on the surface of the seed, worm is controlled with good, insecticidal effect, the materials such as diatomite are recycled to be coated on outermost layer, can reach protection against the tide, improve the effect of seed vitality, efficiently solving traditional seed coat agent cannot be while has diseases prevention concurrently, vermins-proof effect, and clad ratio is low, plant the caducous problem of clothing.
Description
Technical field
The invention discloses a kind of preparation method and applications of double-deck dampproof protection against insects wheat seed coating agent, belong to agricultural materials
Preparing technical field.
Background technology
Seed coat agent is the coating for seeds such as barley, wheat, corn, cotton, paddy rice and forest grass classes, its Main Function
It is to improve breeding quality, improves agroecological environment, integrated control pest and disease damage has micro- fertilizer efficiency concurrently really.Seed coat agent is different from the past
Seed soaking and seed dressing medicament.Seed soaking and dressing seed, the medicament on the surface of the seed meets water after sowing is buried can be dissolved, and drug effect is quickly
Reduce or disappear quickly.And seed coat agent bag can immediately form the film of solidification on seed, seed bury after water-swellable and plant clothing
Will not be dissolved, with the sprouting of seed, germinate, emerge, grow, the effective ingredient planted on clothing can progressively discharge, and by root system
Each position of Assimilation and conductivity plant, extends the term of validity of medicament.Seed pelleting has integrated control crops seedling stage disease pest to endanger,
Drought resisting, cold-proof effect, it is ensured that seedling is saved in once sowing from damage, promote crop growth, nurturing staff to improve yield, improving quality etc. and make
With can typically increase production 10% or so.Seed pelleting is also reduced pesticide dosage, and reduces cost mitigates environmental pollution, is easy to machinery
Operation, saving of work and time economizes on the effect such as kind.
General seed coat agent is made up of two parts, and one is active component, mainly including insecticide, bactericide, hormone, fertilizer or
Beneficial microbe etc., is the active ingredient directly played a role in seed coat agent, and its species, composition and content directly affect seed coat agent
Effect；Two is film forming agent and corresponding auxiliary aid, be maintain seed coat agent physics and chemical property, control clothing film in activity into
Divide the important component of sustained release.Existing seed coat agent active ingredient is single, be formulated it is unstable due to, cause existing kind of clothing
Agent cannot simultaneously prevent and treat crops seedling stage disease and insect pest, and it is low to the clad ratio of seed, to plant clothing easy to fall off.
Beauveria bassiana is a class broad spectrum activity insect pathogenic fungus, with pathogenic strong and broad spectrum disinfestation effect, while by
It is harmless to environment and warm-blooded animal in beauveria bassiana, it is easy to cultivate, raw material is cheap and easy to get, so as to turn into current domestic application most
Extensive insect pathogenic fungus, and China occupies always using the scale of this bacterium preventing and treating agriculture and forestry injurious insect since 20 century 70s
World prostatitis, however, being but rarely seen on the report that beauveria bassiana is used to prepare seed coat agent.
The content of the invention
Present invention mainly solves technical problem：For existing seed coat agent because active ingredient is single, it is unstable to be formulated
The reason for, lead to not prevent and treat crops seedling stage disease and insect pest simultaneously, and it is low to the clad ratio of seed, plant that clothing is caducous to be lacked
Fall into, there is provided a kind of internal layer diseases prevention, control worm, outer layer is moistureproof, the preparation method and applications of the seed coat agent with double-decker, this
Invention first cultivates, collect and obtain beauveria bassiana robe powder, by the addition of beauveria bassiana robe powder in sodium carboxymethylcellulose and
In the transparent liquid that gelatin is prepared, component A is uniformly mixed to obtain, after recycling the materials such as diatomite, talcum powder to crush
Mix powder mixes with neopelex, polyethylene glycol, obtains component B, and component A first is coated on into Wheat Species sublist
Face, sprays component B after not being completely dried, and the double-deck wheat seed for planting clothing film can be carried after being completely dried, and the present invention is sharp
Added in film forming agent with microbial insecticide beauveria bassiana robe powder, be coated on the surface of the seed, controlled worm, kill with good
Worm effect, recycles the materials such as diatomite to be coated on outermost layer, can reach protection against the tide, the effect of seed vitality is improved, by bilayer
It is coated structure and forms fine and close clothing film in the surface of the seed, efficiently solving traditional seed coat agent cannot has diseases prevention, vermins-proof effect, and bag concurrently
Cover rate it is low, plant the caducous problem of clothing, with preferable application prospect.
In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is：
（1）Weigh 100~200g rice to be added in the water of 800~1000g90~95 DEG C, filtered after 40~50min of immersion, must soak
Rice after bubble, to 8~12g edible oils are added in the rice after immersion, sequentially adds 5~7g grapes after being uniformly mixed
Sugar, 0.5~0.7g potassium dihydrogen phosphates, 0.3~0.5g magnesium sulfate, 0.3~0.5g zinc sulfate and 800~1000g deionized waters, stir
Mix it is well mixed after under the conditions of 121 DEG C 15~20min of sterilization treatment, after sterilizing culture medium, and be fitted into culture dish；
（2）A global beauveria bassiana is inoculated with above-mentioned culture dish with 1 μ L oeses, and culture dish is sealed with preservative film, 25~
Cultivated 2~3 days at a temperature of 27 DEG C, 3~5g potassium nitrate, 0.2~0.4g potassium dihydrogen phosphates are added successively in the backward culture dish of culture
With 0.3~0.5g magnesium sulfate, continue to cultivate 3~5 days after sealing, 20~30min of vibration in shaker is added after the completion of culture, shake
Stood after swinging, collect upper strata suspension, by suspension addition centrifugal separator, centrifugation obtains sediment, and is put into baking oven
In, 10~12h is dried at a temperature of 35~40 DEG C, collect to obtain beauveria bassiana robe powder after drying；
（3）4~6g sodium carboxymethylcelluloses are weighed respectively and 3~5g gelatin is added in 600~800mL deionized waters, stir
Transparent liquid is obtained to solid dissolving, to the above-mentioned beauveria bassiana robe powder of addition transparent liquid quality 0.1~0.3% in transparent liquid, and is stirred
Mix well mixed component A；
（4）50~70g diatomite, 20~30g talcum powder, 10~15g humic acid, 30~50g potato starches and 0.3 are weighed respectively
~0.5g potassium dihydrogen phosphates, add crushing in airslide disintegrating mill, cross 500~520 mesh sieves, mixed-powder are obtained, by mixed-powder and 1
~3g neopelexes, the mixing of 10~15g polyethylene glycol, obtain component B after being uniformly mixed.
A kind of concrete application step of double-deck dampproof protection against insects seed coat agent is：
（1）500~700g wheat seeds are weighed, first by wheat seed in mass ratio 1：40 are added in component A, stirring mixing 15
Filtered after~20min, will be filtrated to get wheat seed is placed in drying box, dried to water content at a temperature of 60~70 DEG C and be
10~15%；
（2）Component B is fitted into powder injector, is sprayed to above-mentioned dried wheat seed surface, while injection
Shaken, the rotating speed of shaking is 200~250r/min, and jet velocity is 5~10g/min, and emitted dose is wheat seed quality
5~7%, AEI After End of Injection natural air drying completely, last collecting packing.
After testing, the obtained bilayer dampproof protection against insects seed coat agent of the present invention has splendid cladding and moisture effect, plants clothing not
Easy to fall off, up to more than 98.2%, it is only 0.5~0.8% to be coated expulsion rate to coating uniformity, and the cladding to seed is more firm, while
Seed coat agent of the present invention has preferable prevention and elimination of disease and pests effect, and the control rate to wheat midge, mythimna separata, wheat aphid, wheat sawfly reaches
97.5~99.9%, to wheat rust, head blight, full rot, powdery mildew control rate up to more than 95%, seed coat agent holding effect of the present invention
Time is long, and the lasting period, the wheat after being coated using seed coat agent of the present invention, its germination percentage was improved compared with conventional wheat up to 120~150 days
10~16%, percentage establishment of seedling improves 15~25%, and wheat can be made to increase by 25~35%, and seed coat agent of the present invention has and preferably stores
Stability is deposited, can safe storage 20~30 months under normal temperature sealed environment.
The beneficial effects of the invention are as follows：
（1）Obtained bilayer dampproof protection against insects seed coat agent moisture effect of the invention is good, and preferably, kind clothing is difficult for drop-off, together for covered effect
When kind clothing efficiency time it is long, the bud ratio and planting percent of seed can be effectively improved, so as to reach the purpose of volume increase；
（2）Obtained bilayer dampproof protection against insects seed coat agent good film-forming property of the invention, coating are uniform, stability is strong, can reach in preventing and treating
Pest control while crops seedling stage disease, and be difficult to make insect develop immunity to drugs, with preferable application prospect.
Specific embodiment
Weigh 100~200g rice first to be added in the water of 800~1000g90~95 DEG C, mistake after 40~50min of immersion
Filter, the rice after must soak, to addition 8~12g edible oils in the rice after immersion, sequentially add 5 after being uniformly mixed~
7g glucose, 0.5~0.7g potassium dihydrogen phosphates, 0.3~0.5g magnesium sulfate, 0.3~0.5g zinc sulfate and 800~1000g go from
Sub- water, after being uniformly mixed under the conditions of 121 DEG C 15~20min of sterilization treatment, after sterilizing culture medium, and load culture dish
In；Then a global beauveria bassiana is inoculated with above-mentioned culture dish with 1 μ L oeses, with preservative film sealing culture dish, 25~
Cultivated 2~3 days at a temperature of 27 DEG C, 3~5g potassium nitrate, 0.2~0.4g potassium dihydrogen phosphates are added successively in the backward culture dish of culture
With 0.3~0.5g magnesium sulfate, continue to cultivate 3~5 days after sealing, 20~30min of vibration in shaker is added after the completion of culture, shake
Stood after swinging, collect upper strata suspension, by suspension addition centrifugal separator, centrifugation obtains sediment, and is put into baking oven
In, 10~12h is dried at a temperature of 35~40 DEG C, collect to obtain beauveria bassiana robe powder after drying；Weigh 4~6g carboxylics respectively again
Sodium carboxymethylcellulose pyce and 3~5g gelatin are added in 600~800mL deionized waters, and stirring to solid dissolving obtains transparent liquid, to saturating
The above-mentioned beauveria bassiana robe powder of transparent liquid quality 0.1~0.3% is added in bright liquid, and is uniformly mixed to obtain component A；Finally
50~70g diatomite, 20~30g talcum powder, 10~15g humic acid, 30~50g potato starches and 0.3~0.5g are weighed respectively
Potassium dihydrogen phosphate, adds crushing in airslide disintegrating mill, crosses 500~520 mesh sieves, mixed-powder is obtained, by mixed-powder and 1~3g ten
Dialkyl benzene sulfonic acids sodium, the mixing of 10~15g polyethylene glycol, obtain component B after being uniformly mixed.
A kind of concrete application step of double-deck dampproof protection against insects seed coat agent is：
500~700g wheat seeds are weighed first, first by wheat seed in mass ratio 1：40 are added in component A, stirring mixing
Filtered after 15~20min, will be filtrated to get wheat seed is placed in drying box, dried to water content at a temperature of 60~70 DEG C
It is 10~15%；Then component B is fitted into powder injector, is sprayed to above-mentioned dried wheat seed surface, sprayed
While shaken, the rotating speed of shaking is 200~250r/min, and jet velocity is 5~10g/min, and emitted dose is Wheat Species
The 5~7% of protonatomic mass, AEI After End of Injection natural air drying is complete, last collecting packing.
Example 1
100g rice is weighed first to be added in 800g90 DEG C of water, is filtered after immersion 40min, the rice after must soaking, to immersion
8g edible oils are added in rice afterwards, 5g glucose, 0.5g potassium dihydrogen phosphates, 0.3g sulfuric acid are sequentially added after being uniformly mixed
Magnesium, 0.3g zinc sulfate and 800g deionized waters, after being uniformly mixed under the conditions of 121 DEG C sterilization treatment 15min, after sterilizing
Culture medium, and be fitted into culture dish；Then with 1 μ L oeses, one global beauveria bassiana of inoculation in above-mentioned culture dish, with fresh-keeping
Film seals culture dish, is cultivated 2 days at a temperature of 25 DEG C, and 3g potassium nitrate, 0.2g di(2-ethylhexyl)phosphates are added successively in the backward culture dish of culture
Hydrogen potassium and 0.3g magnesium sulfate, continue to cultivate 3 days after sealing, and 20min is vibrated in addition shaker after the completion of culture, quiet after vibration
Put, collect upper strata suspension, by suspension addition centrifugal separator, centrifugation obtains sediment, and is put into baking oven, 35
10h is dried at a temperature of DEG C, beauveria bassiana robe powder is collected to obtain after drying；4g sodium carboxymethylcelluloses are weighed respectively again and 3g is bright
Glue is added in 600mL deionized waters, and stirring to solid dissolving obtains transparent liquid, is added in transparent liquid quality 0.1% in transparent liquid
Beauveria bassiana robe powder is stated, and is uniformly mixed to obtain component A；50g diatomite, 20g talcum powder, 10g are finally weighed respectively
Humic acid, 30g potato starches and 0.3g potassium dihydrogen phosphates, add crushing in airslide disintegrating mill, cross 500 mesh sieves, obtain mixed-powder,
Mixed-powder and 1g neopelexes, 10g polyethylene glycol are mixed, component B is obtained after being uniformly mixed.
A kind of concrete application step of double-deck dampproof protection against insects seed coat agent is：
500g wheat seeds are weighed first, first by wheat seed in mass ratio 1：40 are added in component A, stirring mixing 15min
After filter, will be filtrated to get wheat seed is placed in drying box, at a temperature of 60 DEG C dry to water content be 10%；Then will
Component B is fitted into powder injector, is sprayed to above-mentioned dried wheat seed surface, is shaken while injection,
The rotating speed of shaking is 200r/min, and jet velocity is 5g/min, and emitted dose is the 5% of wheat seed quality, AEI After End of Injection nature
Air-dry complete, last collecting packing.
Through example detection, the obtained bilayer dampproof protection against insects seed coat agent of the present invention has splendid cladding and moisture effect, plants
Clothing is difficult for drop-off, and up to 98.5%, it is only 0.5% to be coated expulsion rate to coating uniformity, and the cladding to seed is more firm, while of the invention
Seed coat agent has preferable prevention and elimination of disease and pests effect, to wheat midge, mythimna separata, wheat aphid, wheat sawfly control rate up to 97.5%,
To wheat rust, head blight, full rot, powdery mildew control rate up to 96%, seed coat agent efficiency time of the present invention is long, and the lasting period reaches
120 days, the wheat after being coated using seed coat agent of the present invention, its germination percentage was improve 10%, percentage establishment of seedling and improve compared with conventional wheat
15%, wheat can be made to increase by 25%, and seed coat agent of the present invention has preferable bin stability, can be safe under normal temperature sealed environment
Storage 20 months.
Example 2
150g rice is weighed first to be added in 900g93 DEG C of water, is filtered after immersion 45min, the rice after must soaking, to immersion
10g edible oils are added in rice afterwards, 6g glucose, 0.6g potassium dihydrogen phosphates, 0.4g sulphur are sequentially added after being uniformly mixed
Sour magnesium, 0.4g zinc sulfate and 900g deionized waters, after being uniformly mixed under the conditions of 121 DEG C sterilization treatment 18min, after sterilizing
Culture medium is obtained, and is fitted into culture dish；Then with 1 μ L oeses, one global beauveria bassiana of inoculation to above-mentioned culture dish, with guarantor
Fresh film seals culture dish, is cultivated 2 days at a temperature of 267 DEG C, and 4g potassium nitrate, 0.3g phosphoric acid are added successively in the backward culture dish of culture
Potassium dihydrogen and 0.4g magnesium sulfate, continue to cultivate 4 days after sealing, and 25min is vibrated in addition shaker after the completion of culture, quiet after vibration
Put, collect upper strata suspension, by suspension addition centrifugal separator, centrifugation obtains sediment, and is put into baking oven, 38
11h is dried at a temperature of DEG C, beauveria bassiana robe powder is collected to obtain after drying；5g sodium carboxymethylcelluloses are weighed respectively again and 4g is bright
Glue is added in 700mL deionized waters, and stirring to solid dissolving obtains transparent liquid, is added in transparent liquid quality 0.2% in transparent liquid
Beauveria bassiana robe powder is stated, and is uniformly mixed to obtain component A；60g diatomite, 25g talcum powder, 13g are finally weighed respectively
Humic acid, 40g potato starches and 0.4g potassium dihydrogen phosphates, add crushing in airslide disintegrating mill, cross 510 mesh sieves, obtain mixed-powder,
Mixed-powder and 2g neopelexes, 135g polyethylene glycol are mixed, component B is obtained after being uniformly mixed.
A kind of concrete application step of double-deck dampproof protection against insects seed coat agent is：
600g wheat seeds are weighed first, first by wheat seed in mass ratio 1：40 are added in component A, stirring mixing 18min
After filter, will be filtrated to get wheat seed is placed in drying box, at a temperature of 65 DEG C dry to water content be 13%；Then will
Component B is fitted into powder injector, is sprayed to above-mentioned dried wheat seed surface, is shaken while injection,
The rotating speed of shaking is 225r/min, and jet velocity is 8g/min, and emitted dose is the 6% of wheat seed quality, AEI After End of Injection nature
Air-dry complete, last collecting packing.
Through example detection, the obtained bilayer dampproof protection against insects seed coat agent of the present invention has splendid cladding and moisture effect, plants
Clothing is difficult for drop-off, and up to 98.7%, it is only 0.6% to be coated expulsion rate to coating uniformity, and the cladding to seed is more firm, while of the invention
Seed coat agent has preferable prevention and elimination of disease and pests effect, to wheat midge, mythimna separata, wheat aphid, wheat sawfly control rate up to 98.6%,
To wheat rust, head blight, full rot, powdery mildew control rate up to 97%, seed coat agent efficiency time of the present invention is long, and the lasting period reaches
135 days, the wheat after being coated using seed coat agent of the present invention, its germination percentage was improve 13%, percentage establishment of seedling and improve compared with conventional wheat
20%, wheat can be made to increase by 30%, and seed coat agent of the present invention has preferable bin stability, can be safe under normal temperature sealed environment
Storage 25 months.
Example 3
200g rice is weighed first to be added in 1000g95 DEG C of water, is filtered after immersion 50min, the rice after must soaking, to immersion
12g edible oils are added in rice afterwards, 7g glucose, 0.7g potassium dihydrogen phosphates, 0.5g sulphur are sequentially added after being uniformly mixed
Sour magnesium, 0.5g zinc sulfate and 1000g deionized waters, after being uniformly mixed under the conditions of 121 DEG C sterilization treatment 20min, sterilizing
Culture medium is obtained afterwards, and is fitted into culture dish；Then with 1 μ L oeses, one global beauveria bassiana of inoculation to above-mentioned culture dish, use
Preservative film seals culture dish, is cultivated 3 days at a temperature of 27 DEG C, and 5g potassium nitrate, 0.4g phosphorus are added successively in the backward culture dish of culture
Acid dihydride potassium and 0.5g magnesium sulfate, continue to cultivate 5 days after sealing, 30min are vibrated in addition shaker after the completion of culture, after vibration
Stand, collect upper strata suspension, by suspension addition centrifugal separator, centrifugation obtains sediment, and is put into baking oven,
12h is dried at a temperature of 40 DEG C, beauveria bassiana robe powder is collected to obtain after drying；Weigh 6g sodium carboxymethylcelluloses and 5g respectively again
Gelatin is added in 800mL deionized waters, and stirring to solid dissolving obtains transparent liquid, to addition transparent liquid quality 0.3% in transparent liquid
Above-mentioned beauveria bassiana robe powder, and it is uniformly mixed to obtain component A；Finally weigh respectively 70g diatomite, 30g talcum powder,
15g humic acid, 50g potato starches and 0.5g potassium dihydrogen phosphates, add crushing in airslide disintegrating mill, cross 520 mesh sieves, obtain mixed powder
End, mixed-powder and 3g neopelexes, 15g polyethylene glycol are mixed, and component B is obtained after being uniformly mixed.
A kind of concrete application step of double-deck dampproof protection against insects seed coat agent is：
700g wheat seeds are weighed first, first by wheat seed in mass ratio 1：40 are added in component A, stirring mixing 20min
After filter, will be filtrated to get wheat seed is placed in drying box, at a temperature of 70 DEG C dry to water content be 15%；Then will
Component B is fitted into powder injector, is sprayed to above-mentioned dried wheat seed surface, is shaken while injection,
The rotating speed of shaking is 250r/min, and jet velocity is 10g/min, and emitted dose is the 7% of wheat seed quality, and AEI After End of Injection is certainly
So air-dry complete, last collecting packing.
This example operation is easy, and when using, after testing, the obtained bilayer dampproof protection against insects seed coat agent of the present invention has splendid
Cladding and moisture effect, kind clothing are difficult for drop-off, and up to 99.2%, it is only 0.8% to be coated expulsion rate to coating uniformity, to the cladding of seed
More firmly, while seed coat agent of the present invention has preferable prevention and elimination of disease and pests effect, to wheat midge, mythimna separata, wheat aphid, wheat sawfly
Control rate up to 99.9%, to wheat rust, head blight, full rot, powdery mildew control rate up to 98%, seed coat agent holding effect of the present invention
Time is long, and the lasting period, the wheat after being coated using seed coat agent of the present invention, its germination percentage was improve compared with conventional wheat up to 150 days
16%th, percentage establishment of seedling improves 25%, and wheat can be made to increase by 35%, and seed coat agent of the present invention has preferable bin stability, in normal temperature
Can safe storage 30 months under sealed environment.
Claims (2)
1. a kind of preparation method and applications of double-deck dampproof protection against insects wheat seed coating agent, it is characterised in that specific preparation process is：
（1）Weigh 100~200g rice to be added in the water of 800~1000g90~95 DEG C, filtered after 40~50min of immersion, must soak
Rice after bubble, to 8~12g edible oils are added in the rice after immersion, sequentially adds 5~7g grapes after being uniformly mixed
Sugar, 0.5~0.7g potassium dihydrogen phosphates, 0.3~0.5g magnesium sulfate, 0.3~0.5g zinc sulfate and 800~1000g deionized waters, stir
Mix it is well mixed after under the conditions of 121 DEG C 15~20min of sterilization treatment, after sterilizing culture medium, and be fitted into culture dish；
（2）A global beauveria bassiana is inoculated with above-mentioned culture dish with 1 μ L oeses, and culture dish is sealed with preservative film, 25~
Cultivated 2~3 days at a temperature of 27 DEG C, 3~5g potassium nitrate, 0.2~0.4g potassium dihydrogen phosphates are added successively in the backward culture dish of culture
With 0.3~0.5g magnesium sulfate, continue to cultivate 3~5 days after sealing, 20~30min of vibration in shaker is added after the completion of culture, shake
Stood after swinging, collect upper strata suspension, by suspension addition centrifugal separator, centrifugation obtains sediment, and is put into baking oven
In, 10~12h is dried at a temperature of 35~40 DEG C, collect to obtain beauveria bassiana robe powder after drying；
（3）4~6g sodium carboxymethylcelluloses are weighed respectively and 3~5g gelatin is added in 600~800mL deionized waters, stir
Transparent liquid is obtained to solid dissolving, to the above-mentioned beauveria bassiana robe powder of addition transparent liquid quality 0.1~0.3% in transparent liquid, and is stirred
Mix well mixed component A；
（4）50~70g diatomite, 20~30g talcum powder, 10~15g humic acid, 30~50g potato starches and 0.3 are weighed respectively
~0.5g potassium dihydrogen phosphates, add crushing in airslide disintegrating mill, cross 500~520 mesh sieves, mixed-powder are obtained, by mixed-powder and 1
~3g neopelexes, the mixing of 10~15g polyethylene glycol, obtain component B after being uniformly mixed.
2. preparation method and applications of a kind of double-deck dampproof protection against insects seed coat agent according to claim 1, it is characterised in that：
Concrete application step is：
（1）500~700g wheat seeds are weighed, first by wheat seed in mass ratio 1：The 40 component A for being added to claim 1
In, stirring mixing 15~20min after filter, will be filtrated to get wheat seed is placed in drying box, at a temperature of 60~70 DEG C
It is 10~15% to dry to water content；
（2）The component B of claim 1 is fitted into powder injector, is sprayed to above-mentioned dried wheat seed surface,
Shaken while injection, the rotating speed of shaking is 200~250r/min, and jet velocity is 5~10g/min, and emitted dose is small
The 5~7% of wheat seeds protonatomic mass, AEI After End of Injection natural air drying is complete, last collecting packing.
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| CN201611211719.5A CN106818886A (en) | 2016-12-25 | 2016-12-25 | A kind of preparation method and applications of double-deck dampproof protection against insects wheat seed coating agent |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111265417A (en) * | 2020-02-12 | 2020-06-12 | 东莞市星泽日用品有限公司 | Efficient moisture-proof coated bath salt ball and preparation method thereof |
| WO2024043794A1 (en) * | 2022-08-25 | 2024-02-29 | Donaghys Limited | Seed composition and related methods of manufacture and use |
Citations (4)
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| CN111265417A (en) * | 2020-02-12 | 2020-06-12 | 东莞市星泽日用品有限公司 | Efficient moisture-proof coated bath salt ball and preparation method thereof |
| WO2024043794A1 (en) * | 2022-08-25 | 2024-02-29 | Donaghys Limited | Seed composition and related methods of manufacture and use |
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