CN104488869B - A kind of bactericidal composition of the ethyl phenylacrylate of 2 cyano group, 3 amino 3 and Osthole - Google Patents
A kind of bactericidal composition of the ethyl phenylacrylate of 2 cyano group, 3 amino 3 and Osthole Download PDFInfo
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Abstract
The invention discloses a kind of ethyl phenylacrylate of 2 cyano group, 3 amino 3 and the bactericidal composition of Osthole, active component includes in described bactericidal composition：Component A：The ethyl phenylacrylate of 2 cyano group, 3 amino 3；B component：Osthole；Wherein, the weight ratio of component A and B component is 0.04~12.8：1；Bactericidal composition of the present invention containing the ethyl phenylacrylate of 2 cyano group, 3 amino 3 and Osthole, is not only demonstrated by the premium properties of two single doses of 2-cyano-3-amino-3-phenylancryic acetate and Osthole respectively, also shows synergy effect；This bactericidal composition when occurring the disease same period such as wheat scab, wheat powdery mildew, can reach it is simultaneous control purpose, the pathogen of infected seed is also played when handling rice paddy seed and simultaneous controls effect.
Description
Technical field
The present invention relates to the compounded technology field of agricultural chemicals, more particularly to a kind of 2- cyano group -3- amino -3- phenylacrylic acid second
The bactericidal composition of ester and Osthole.
Background technology
Bactericide 2- cyano group -3- amino -3- ethyl phenylacrylates, Universal Chinese character name：2-cyano-3-amino-3-phenylancryic acetate, is new cyano group
Acrylic bactericide, is mainly used in preventing and treating by important diseases such as the microbial wheat scab of F.graminearum schw, bakanaes disease of rice
Evil.Current Patents existing on the market are 1：2- cyano group -3- substituted phenylacrylate compounds, composition and its system
Preparation Method and the application on crop bactericide, ZL01115593.0.Patent 2：Containing compound 2- cyano group -3- amino -
The bactericidal composition of 3- ethyl phenylacrylates, ZL200410014097.8.Patent 3：- 3- amino-the 3- of cyano group containing 2- phenyl third
Bactericidal composition of olefin(e) acid ethyl ester and propiconazole and application thereof, ZL200710020277.0.Patent 4：Prevent and treat bakanae disease of rice
Composition pesticide, ZL200410065145.6.Patent 5：2- cyano group -3- amino -3- ethyl phenylacrylates prevent and treat crop disease
Harmful application, ZL200610125921.6.Patent 6：The preparation method of 2- cyano-3-amino acrylic ester derivates,
ZL200810235717.9.2-cyano-3-amino-3-phenylancryic acetate has been enter into scale industrialization exploitation, and achieves good economic benefit and society's effect
Benefit.
2-cyano-3-amino-3-phenylancryic acetate has special efficacy to the wheat scab as caused by Fusarium graminearum, to wheat scab, bakanae disease of rice
It is effective Deng disease caused by sickle-like bacteria.With protection, therapeutic action, the wheat scab of resistance is particularly produced to carbendazim
Same effective, the bakanae disease of rice for producing resistance to Prochloraz is equally effective.Research shows that 2-cyano-3-amino-3-phenylancryic acetate prevents and treats wheat scab
Activity be better than conventional medicament carbendazim, its preventing and treating for being found to be wheat scab, which is provided, to be substituted or wheel medicament.Meanwhile,
Because carbendazim is always the leading medicament of wheat scab preventing and treating, the different degrees of resistance to the action of a drug is generated in different zones, and
The same efficient characteristic of 2-cyano-3-amino-3-phenylancryic acetate antagonism bacterial strain, is highly advantageous to and provides excellent agent for the preventing and treating of wheat scab.
2-cyano-3-amino-3-phenylancryic acetate unique structure, mechanism of action is novel, is the special effect agent for administering fusarium graminearum, with " ill
Diseases prevention, disease-free volume increase " and the characteristics of red mould toxin in wheat is greatly reduced.The product is by marketing for many years, using model
Enclose wide.
The experiment of agricultural chemicals Hygiene Toxicology shows that 2-cyano-3-amino-3-phenylancryic acetate belongs to lower toxicity agricultural chemicals, and 25% suspending agent belongs to micro-virus kind agricultural chemicals, anxious
Property oral and percutaneous LD50 be all higher than 5000mg/kg, it is non-stimulated to eyes and skin.Environmental evaluation, which is tested, to be shown, 25%
Suspending agent is to honeybee, silkworm low toxicity, to fish, bird poisoning.To silkworm low-risk, in honeybee to excessive risk.Note when using pair
Fish and the influence of honeybee.
2-cyano-3-amino-3-phenylancryic acetate has been achieved for the formal registration in wheat scab and bakanae disease of rice, 25% 2-cyano-3-amino-3-phenylancryic acetate SC,
Consumption 375-750gai/ha is recommended in registration number PD20121670, wheat scab spraying, and consumption is recommended in bakanae disease of rice seed soaking
83.3-125mg/kg.48%（12+36）Tebuconazole 2-cyano-3-amino-3-phenylancryic acetate SC, registration number PD20141746, wheat scab recommends to use
Measure 288-432gai/ha.20%（5+15）Hexaconazole 2-cyano-3-amino-3-phenylancryic acetate SC, registration number PD20141067, wheat powdery mildew is recommended
Consumption 240-420gai/ha；Registration number LS20130506, wheat scab recommends consumption 240-330gai/ha.
Osthole, osthol, chemical name：8-（3- methyl this 2- cyclobutenyls）Methyl ether San shape ketone, belongs to Coumarins
Compound.Osthole medically has wider purposes.The researchers such as Shi Zhiqi report Osthole to gibberella saubinetii
The part result of study of germ, Osthole can suppress gibberella mycelia apical growth, suppress the increase of mycelia branch, suppress spore
Sub- germ tube elongation is sprouted, and suppresses absorption of the fusarium graminearum to glucose, participates in suppressing the energy generation of fusarium graminearum
Thank that [Shi Zhiqi, Shen Shouguo, Xu Langlai, Fan Yongjian, Osthole is to the Primary Study of plant pathogenic fungi suppression mechanism, agricultural chemicals
Learn journal, 2004,6（4）：28-32], [Shen Shouguo, Shi Zhiqi, Xu Langlai, Fan Yongjian, Osthole is to fusarium graminearum Portugal
The suppression of grape sugar, calcium uptake and ATPase activity, Pesticide Science journal, 2005,7（2）：135-139].
Agriculturally it is being registered for being used to prevent and treat powdery mildew of cucumber [registration number PD20121586, recommendation consumption 22.5-
30gai/ha], cowpea powdery mildew [registration number LS20140092, recommend consumption 5-6.67mg/kg], rice green smut [registration number
PD20141075, recommends consumption 19-25gai/ha], it is used to prevent and treat rice sheath blight disease [registration number with composite jinggangmycin
LS20140106, the Osthole of 11.8% jinggangmycin A+0.2% recommends consumption 90-108gai/ha].Osthole can also have in
Prevent and treat cabbage caterpillar or tea geometrid [registration number PD20121348,4.8-7.2gai/ha].
Wheat scab, wheat powdery mildew etc. are the important diseases in Wheat Production.Wheat scab in the late three decades
Preventing and treating relies on benzimidazole series bactericidal agent, especially carbendazim always, and long-term use has generated medium or height in some areas
Spend the resistance to the action of a drug.It is used for administering the resistance to the action of a drug in the urgent need to effective agent enters road wheel.Novel cyano acrylic bactericide：Cyanogen alkene
Bacterium ester is the specific drug for preventing and treating wheat scab and resisting carbendazim wheat scab, and it has weight to improving wheat yield and quality
Want meaning.
The preventing and treating of wheat powdery mildew uses triazole bactericidal agent mostly, with the industrialized production of Osthole at home and
Application and development, starts to be applied on the crops such as wheat, paddy rice, fruits and vegetables.Therefore, it is former from the information system of crop pest distribution in China comprehensive regulation
Then set out, have selected 2-cyano-3-amino-3-phenylancryic acetate and carry out synergy research with Osthole, screen compound synergic formula, be that agricultural production is carried
For wide spectrum, novel germicide kind efficiently, safe, to improve agricultural production efficiency, it is ensured that provide high-quality source agricultural product and provide
High-quality agricultural material product, prevents and cures diseases in wheat, paddy rice etc. produce, adjusts promotion growth, and will effectively improve agricultural production quality
Amount and yield.
The content of the invention
For above-mentioned problem, present invention aims at provide a kind of 2- for agricultural productions such as wheat, paddy rice, fruits and vegetables
The bactericidal composition of cyano group -3- amino -3- ethyl phenylacrylates and Osthole, is mainly used in preventing and treating wheat scab, small
Wheat powdery mildew, take-all etc., prevent and treat bakanae disease of rice, rice sheath blight disease, rice green smut etc., it is ensured that wheat, paddy rice etc.
Plant growth, improves wheat, the yield and quality of paddy rice.
In order to achieve the above object, the technical solution adopted by the present invention is as follows：A kind of 2- cyano group -3- amino -3- phenyl third
Active component includes in the bactericidal composition of olefin(e) acid ethyl ester and Osthole, described bactericidal composition：
Component A：2- cyano group -3- amino -3- ethyl phenylacrylates；
B component：Osthole；
Wherein, the weight ratio of component A and B component is 0.04~12.8：1.
The weight ratio of component A and B component is 0.1~10 in bactericidal composition of the present invention：1.
2-cyano-3-amino-3-phenylancryic acetate（2- cyano group -3- amino -3- ethyl phenylacrylates）To reaping hooks such as wheat scab, bakanaes disease of rice
Microbial disease has efficient.To the in vitro bactericidal activity of wheat scab, bakanae disease of rice（EC50Value）Respectively
0.1411、0.4592 ug/ml.Bactericidal action mode, which is studied, to be shown, the bactericide has protection, therapeutic action.Field efficacy is tried
Test research to show, use 12.5 ~ 37.5g/ mu at wheat flowering initial stage, even spraying, it is extremely effective to prevent and treat wheat scab, anti-
Effect is better than the carbendazim with dosage, and to producing resistance（Resisting carbendazim）Area it is same effective.It is outstanding using 25% 2-cyano-3-amino-3-phenylancryic acetate
2000 ~ 4000 times of liquid of floating agent, seed soaking rice seed can effectively prevent and treat bakanae disease of rice, and preventive effect is better than the common medicine with dosage
Agent Prochloraz.But 2-cyano-3-amino-3-phenylancryic acetate is substantially invalid to wheat powdery mildew.
In addition, the active research for 2-cyano-3-amino-3-phenylancryic acetate to wheat scab sensitive strain and resistant strain, as a result shows：
2-cyano-3-amino-3-phenylancryic acetate is to sensitive strain and the EC of resistant strain50Respectively 0.1415 and 0.1760 ug/ml；EC95Respectively 0.9224
With 0.8201 ug/ml.And carbendazim is to identical sensitive strain and the EC of resistant strain50Respectively 0.4225 and 7.3952mg/L；
EC95Respectively 0.8324 and 34.4299mg/L.Carbendazim has good activity to sensitive strain, and the work of antagonism bacterial strain
Property is poor.Thus, further demonstrate that 2-cyano-3-amino-3-phenylancryic acetate is respectively provided with efficiently to sensitive strain and resistant strain.
Osthole belongs to biological pesticide class, and inventor's research shows：Osthole is efficient to wheat powdery mildew, greenhouse wheat
During potted plant test, 100 ug/ml preventive effect is up to 100%.Osthole has excellent activity to wheat scab, to sensitive strain
EC50For 0.4563 ug/ml, EC90For 20.6207 ug/ml.Osthole is better than to the bacteriostatic activity of fusarium graminearum
The biological pesticides such as waxy Bacillus, polyoxin, jinggangmeisu, kasugarnycin, but not as good as 2-cyano-3-amino-3-phenylancryic acetate.
It is wheat scab, wheat powdery mildew, take-all, paddy rice as the research object of the harmful organism of the present invention
Bakanae disease, rice sheath blight disease, rice green smut etc., are that serious disease occurs for the crop fields such as wheat or paddy rice.It is general to require
Medicament is preferably provided with the characteristics such as protection, treatment, interior suction and infiltration.2-cyano-3-amino-3-phenylancryic acetate has the effects such as protection, treatment, Osthole tool
There is the effect such as protection, treatment, infiltration.Therefore, the novel formulation that compounded combination is formed has the characteristics such as protection, treatment, infiltration, symbol
Close the requirement of actual medication.
The present invention is in synergy to the synergy of wheat scab sensitive strain with Osthole in 2-cyano-3-amino-3-phenylancryic acetate or is added
Effect：Using 2-cyano-3-amino-3-phenylancryic acetate and Osthole, made a living survey target, test and evaluation method reference with wheat scab sensitive strain
NY/T1156.2-2006 farm-chemical indoor determination test rule bactericide part 2s：Suppress disease fungus mycelial growth
Test Plating, the part of bactericide the 6th：The synergy of mixture is determined, and have rated 2-cyano-3-amino-3-phenylancryic acetate and Osthole combines work
With type, as a result show：2-cyano-3-amino-3-phenylancryic acetate and Osthole 12.8 ~ 0.04：In the range of 1, synergy is in addition or synergistic effect
Deng.Ratio optimization screening shows, when 10 ~ 0.1：Synergistic effect is shown as when 1.
The present invention is presented synergy or be added with Osthole in 2-cyano-3-amino-3-phenylancryic acetate to the synergy of wheat powdery mildew cooperates with work
With：Using 2-cyano-3-amino-3-phenylancryic acetate and Osthole, made a living survey target with wheat powdery mildew, test and evaluation method with reference to NY/
The part of T1156.4-2006 farm-chemical indoor determination test rules bactericide the 4th：Prevent and treat wheat powdery mildew experiment potted plant
24h connects bacterium after method, spray, tests protective effect.Osthole for preventing and treating wheat powdery mildew is efficient, when dosage is 100,10 ug/ml
8 days preventive effects are respectively 100,90% after spraying.2-cyano-3-amino-3-phenylancryic acetate preventing and treating wheat powdery mildew effect is low, preventive effect when dosage is 100ug/ml
For 60%.When 10 ~ 0.1 parts of 2-cyano-3-amino-3-phenylancryic acetate and 1 part of compounded combination of Osthole, the preventive effect of each combined treatment is superior to frutus cnidii
Prevention effect during plain single dose same dose.Show, both synergy show synergy or are added synergy.
Bactericidal composition of the present invention can be configured to the water dispersible granules agriculturally allowed, suspending agent, aqueous emulsion, can
WP and missible oil.
From the analysis of physical and chemical feature of two effective ingredients, in actual production process, moisture can be made into and dissipated
Granula or the formulation such as suspending agent or aqueous emulsion or wettable powder or missible oil.To reach emulsifiability and excellent in stability, to planting
The adhesion plate-out ability of thing is strong, the good requirement of infiltration conductibility.
, can be by 2- cyanogen during the bactericidal composition of the preparation -3- of cyano group containing 2- amino -3- ethyl phenylacrylates and Osthole
Base -3- amino -3- ethyl phenylacrylates, Osthole and appropriate carrier and additive, such as emulsifying agent, dispersant, stably
Agent, bleeding agent etc. are mixed, and water dispersion granule, suspending agent, aqueous emulsion, wettable powder or missible oil etc. is made by common method.
The carrier for being adapted to use in composition of the present invention with bactericidal activity can be typically used for any of agriculture and garden medicament
A kind of solid or liquid-carrier, as needed, can be used various surfactants, stabilizer and other auxiliary ingredients.In business
In preparation, bactericidal composition of the invention can also be mixed with other active ingredients（Such as various insecticides, acaricide,
Bactericide, herbicide, plant growth regulator, synergist, fertilizer, aromatic）, to extend its practicality.
Bactericidal composition of the present invention can be used for administering wheat scab, wheat powdery mildew, take-all, paddy rice
Bakanae disease, rice sheath blight disease and rice green smut.
- the 3- of cyano group containing the 2- amino -3- ethyl phenylacrylates of the present invention and the bactericidal composition of Osthole are to wheat
The important disease such as head blight, wheat powdery mildew, take-all, bakanae disease of rice, rice sheath blight disease, rice green smut has excellent
Good preventive effect.Although the amount of application of bactericidal composition of the present invention need to according to essential condition such as population density, the species of core crop and
Cropping pattern, weather condition, method of application etc. and adjust, but in general, per hectare apply total amount be 0.1 ~ 1000g, it is excellent
Choosing for 1 ~ 500g.Crop protection practice in, when bactericidal composition of the present invention be water-dispersible granule, suspending agent, aqueous emulsion,
When wettable powder, missible oil etc., imposed on after being diluted by corresponding effective ingredient consumption on crop and harmful organism.
Because 2-cyano-3-amino-3-phenylancryic acetate also significantly reduces the amount of red mould toxin in wheat while preventing and curing diseases, therefore protecting
While wheat is protected from harm, yield is not only increased, and improves wheat quality, it is food-safe highly beneficial.
The advantage of the invention is that：
1）The bactericidal composition of-the 3- of cyano group containing 2- amino -3- ethyl phenylacrylates and Osthole of the invention, wide spectrum,
Efficiently, safety, excellent and safe new type bactericide is provided for crop productions such as wheat, paddy rice.
The bactericidal composition of-the 3- of cyano group containing 2- amino -3- ethyl phenylacrylates and Osthole of the invention, is demonstrated by cyanogen
The premium properties of two single doses of alkene bacterium ester and Osthole, also shows synergy effect.
Wide spectrum：This bactericidal composition expands fungicidal spectrum compared with single dose, to wheat scab, wheat powdery mildew, wheat
The important disease such as full rot, bakanae disease of rice, rice sheath blight disease, rice green smut has efficiently.
Efficiently：This bactericidal composition has significant synergies compared with single 2-cyano-3-amino-3-phenylancryic acetate or Osthole, this
The consumption that single dose is reduced in production practices is shown, preventive effect is improved.
Safety：This bactericidal composition is a kind of environment-friendly type, high performance novel built bactericide, and effective ingredient is micro- in itself
Poison, formulation environmental protection is safe to the producer and user.Effect and the Osthole life of red mould toxin are reduced plus 2-cyano-3-amino-3-phenylancryic acetate
The characteristics of thing agricultural chemicals so that this bactericidal composition more favor of crop protection the characteristics of in terms of security.
2）Delay the medicament resistance to the action of a drug；Because the use of single medicament can increase medicament selection pressure, easily make harmful organism
Develop immunity to drugs, the thing followed is exactly drug effect reduction or prevents and treats invalid.Therefore, from protection novel fungicides agent 2-cyano-3-amino-3-phenylancryic acetate and excellent
The angle of good bactericide Osthole is set out, and is developed the economic life that its complex preparation is conducive to protecting new varieties, is reduced single dose
Consumption, mitigates medicament selection pressure, delays drug-fast generation or development.
3）Science is compounded, and decrement is used, and reduces cost.This bactericidal composition has synergistic effect, therefore, not only expand
Fungicidal spectrum, and the effective dose of medicament is reduced, medicament service efficiency is improved, a mu drug cost is reduced.
This bactericidal composition when occurring the disease same period such as wheat scab, wheat powdery mildew, can reach it is simultaneous control purpose, together
Sample, also plays to the pathogen of infected seed when handling rice paddy seed and simultaneous controls effect；This bactericidal composition is compounded through science
After can save dispenser cost of labor.
Embodiment
With reference to embodiment, the present invention is described in further detail.
In the following embodiments, it is to-the 3- of cyano group containing 2- amino -3- ethyl phenylacrylates of the invention and Osthole
Bactericidal composition is further described, but its active ingredient and its proportioning, auxiliary ingredients and its addition etc. be not limited to
Lower embodiment.In addition, number all in embodiment is by weight.
Embodiment 1：The bactericidal composition of the present invention is configured to agriculturally using the processing mode and auxiliary material of conventional preparation
The water dispersible granules of permission, its detailed formula is as follows：
The mass concentration that the present embodiment obtains active component is 55% water dispersible granules, according to the method for agriculture plant protection,
1000 ~ 5000 times of dilution is watered, the crop diseases such as wheat scab, wheat powdery mildew, rice green smut, preventive effect can be effectively prevented and treated
More than 90% can be reached, not only disease-preventing and yield-increasing, and red mould toxin in wheat can be reduced, improve wheat quality.Water dispersible granules belong to
Environment-friendly formulation, effective ingredient is not limited to the 55% of the present embodiment, can be 10 ~ 70%；The combination of effective ingredient is not limited to
, can be 0.1~10 in 5 parts of 50 parts of 2- cyano group this 3- this 3- of amino ethyl phenylacrylates plus Osthole：It is preferably any in 1
Proportioning；Auxiliary agent is also not limited to used in the present embodiment, can select other suitable auxiliary agents.
Embodiment 2：The bactericidal composition of the present invention is configured to agriculturally using the processing mode and auxiliary material of conventional preparation
The suspending agent of permission, its detailed formula is as follows：
The suspending agent of the present embodiment 30%, according to the method for agriculture plant protection, is watered 1500 ~ 5000 times of dilution, can effectively prevent
The crop diseases such as wheat scab, wheat powdery mildew, rice green smut are controlled, anti-efficiency reaches more than 85%, not only disease-preventing and yield-increasing,
And red mould toxin in wheat can be reduced, improve wheat quality.Seed treatment is also used as, bakanae disease of rice, wheat is prevented and treated
Root rot etc..Suspending agent belongs to environment-friendly formulation, and effective ingredient is not limited to the 30% of the present embodiment, can be 5 ~ 50%；Effectively
The combination of composition is not limited to 25 parts of 5 parts of 2- cyano group this 3- this 3- of amino ethyl phenylacrylates plus Osthole, can be 0.1
~10：Preferably any proportioning in 1；Auxiliary agent is also not limited to used in the present embodiment, can select other suitable auxiliary agents.
Embodiment 3：The bactericidal composition of the present invention is configured to agriculturally using the processing mode and auxiliary material of conventional preparation
The aqueous emulsion of permission, its detailed formula is as follows:
The mass concentration that the present embodiment obtains active component is 5% aqueous emulsion, according to the method for agriculture plant protection, is watered
500 ~ 2500 times of dilution, can effectively prevent and treat the crop diseases such as wheat scab, wheat powdery mildew, rice green smut, anti-efficiency reaches
To more than 80%, not only disease-preventing and yield-increasing, and can reduce red mould toxin in wheat improves wheat quality.Aqueous emulsion belongs to environment-friendly
Formulation, effective ingredient is not limited to the 5% of the present embodiment, can be 5 ~ 50%；The combination of effective ingredient is not limited to 2- cyano group sheets
2.5 parts of 2.5 parts of 3- this 3- of amino ethyl phenylacrylates plus Osthole, can be 0.1~10：Preferably any proportioning in 1；Help
Agent is also not limited to used in the present embodiment, can select other suitable auxiliary agents.
Embodiment 4：The bactericidal composition of the present invention is configured to agriculturally using the processing mode and auxiliary material of conventional preparation
The wettable powder of permission, its detailed formula is as follows：
The mass concentration that the present embodiment obtains active component is 12% wettable powder, according to the method for agriculture plant protection,
500 ~ 3000 times of dilution is watered, the crop diseases such as wheat scab, wheat powdery mildew, rice green smut, preventive effect can be effectively prevented and treated
More than 85% can be reached, not only disease-preventing and yield-increasing, and red mould toxin in wheat can be reduced, improve wheat quality.In wettable powder
Effective ingredient is not limited to the 12% of the present embodiment, can be 5 ~ 50%；The combination of effective ingredient is not limited to 2- cyano group this 3- ammonia
Basic 10 parts of 3- ethyl phenylacrylates add 2 parts of Osthole, can be 0.1~10：Preferably any proportioning in 1；Auxiliary agent is not yet
It is confined to used in the present embodiment, other suitable auxiliary agents can be selected.
Embodiment 5：The bactericidal composition of the present invention is configured to agriculturally using the processing mode and auxiliary material of conventional preparation
The missible oil of permission, its detailed formula is as follows：
The mass concentration that the present embodiment obtains active component is 11% missible oil, according to the method for agriculture plant protection, is watered dilute
500 ~ 3000 times are released, the crop diseases such as wheat scab, wheat powdery mildew, rice green smut can be effectively prevented and treated, anti-efficiency reaches
More than 80%, not only disease-preventing and yield-increasing, and can reduce red mould toxin in wheat improves wheat quality.Effective ingredient not office in missible oil
It is limited to the 11% of the present embodiment, can is 5 ~ 70%；The combination of effective ingredient is not limited to 2- cyano group this 3- this 3- of amino phenyl third
10 parts of 1 part of olefin(e) acid ethyl ester plus Osthole, can be 0.1~10：Preferably any proportioning in 1；Auxiliary agent is also not limited to this implementation
Used in example, other suitable auxiliary agents can be selected.
According to specific embodiment to the present invention -3- of cyano group containing 2- amino -3- ethyl phenylacrylates and Osthole
The practical function of bactericidal composition is tested.
Effect test 1：2-cyano-3-amino-3-phenylancryic acetate is to wheat scab sensitive strain and the bacteriostatic activity of resistant strain；
Strains tested：Fusarium graminearum, sensitive strain G4, resistant strain G2, this laboratory cultures are preserved.
Test method：With reference to NY/T1156.2-2006 farm-chemical indoor determination test rule bactericide part 2s：
Suppress disease fungus mycelial growth experiment Plating.
It is prepared by pastille culture medium：Treatment agent and comparison medicament acetone solution, are configured to mother liquor.According to certain concentration
Gradient, is separately added into test medicament and comparison medicament sterilized and is cooled to mixing in 45 DEG C or so of culture medium, pour into flat
Ware, is made pastille flat board.Toxicity test：Using colony diameter method.A bacterium dish is connect in pastille flat board center, mycelia is down.25℃
When lower culture to blank control bacterium colony is covered with culture dish more than 2/3, the colony diameter of each processing is measured.Each bacterium colony is handed over by cross
Fork method is measured 2 times, and the size of bacterium colony is represented with its average.With formula（1）Calculate inhibiting rate of the medicament to thalli growth.
Statistical analysis is carried out to proof load and thalli growth inhibiting rate using DPS softwares, Toxicity baseline, EC is obtained50、EC95
Value.Result of the test：The Toxicity baseline such as table 1 of 2-cyano-3-amino-3-phenylancryic acetate, carbendazim to sensitive strain G4 and resistant strain G2.
Test result shows：2-cyano-3-amino-3-phenylancryic acetate has very high bacteriostatic activity to wheat scab, to sensitive strain and resistance bacterium
The EC of strain50Respectively 0.1415 and 0.1760 ug/ml；EC95Respectively 0.9224 and 0.8201 ug/ml.Activity is in same number
Magnitude, further demonstrates that 2-cyano-3-amino-3-phenylancryic acetate is respectively provided with efficiently to sensitive strain and resistant strain.Carbendazim has to wheat scab
Activity, to sensitive strain and the EC of resistant strain50Respectively 0.4225 and 7.3952mg/L；EC95Respectively 0.8324 He
34.4299mg/L.Carbendazim has good activity to sensitive strain, and antagonism bacterial strain is active poor.
Effect test 2：Protection and therapeutic action of the 2-cyano-3-amino-3-phenylancryic acetate to wheat scab；
Material to be tested：Fusarium graminearum G4（Sensitive strain）.
Test method：Protective effect method of testing：First 2-cyano-3-amino-3-phenylancryic acetate, carbendazim series concentration sample are uniformly sprayed at
Wheat（Two leaf stage）On, after processing one day, with first blade middle and lower part of major part acupuncture wheat, two holes on every blade,
Fresh mycelia block is taken again（Diameter 5mm）Being bonded on blade has at hole, and often processing sets 8 repetitions, uses moisture preserving cover moisturizing, normal light
Line, 26 DEG C of temperature, humidity more than 95%.Checkout facility result after four days, measures scab length, calculates inhibiting rate.Therapeutic action is surveyed
Method for testing：First use major part acupuncture wheat（Two leaf stage）First blade middle and lower part, two holes on every blade, then take fresh bacterium
Silk block（Diameter 5mm）Being bonded on blade has at hole, and often processing sets 8 repetitions, uses moisture preserving cover moisturizing, ordinary ray, 26 DEG C of temperature,
Humidity more than 95%.After inoculation one day, compound ii -1, carbendazim series concentration sample are uniformly sprayed on wheat, 3 days
Checkout facility result, measures scab length afterwards, calculates inhibiting rate.
Result of the test：Test result such as table 2 of the 2-cyano-3-amino-3-phenylancryic acetate to protective effect and the therapeutic action of wheat scab.From table
Understand, compound 2-cyano-3-amino-3-phenylancryic acetate, which has, to be protected and treatment double action, and protective effect is better than therapeutic action.
Effect test 3：Active testing of the 2-cyano-3-amino-3-phenylancryic acetate to take-all；
For examination target：Gaeumannomyces graminis, indoor cultured in vitro.
Test method：Reference test 1, in vitro Plating.
Result of the test：2-cyano-3-amino-3-phenylancryic acetate has indoor isolated activity, EC to take-all50For 61.6343 μ g/ml, EC90
For 141.0519 μ g/ml.EC of the comparison medicament Difenoconazole to take-all50For 0.4213 μ g/ml, EC90For 3.4721
μg/ml.Such as table 3.
Effect test 4：Active testing of the biological pesticides such as Osthole to wheat scab；
Strains tested：Wheat scab（Gibbrella zeae）, sensitive strain G4, the preservation of this laboratory cultures.
Test method：Reference test 1, in vitro Plating.
Result of the test：In order to screen the biological pesticide with greater activity, have selected Osthole, waxy Bacillus,
5 kinds of biological pesticides such as jinggangmeisu, kasugarnycin, polymycin give birth to comparative test in the same terms reason progress, as a result show：To small
Wheat head blight activity highest is Osthole, and the activity of waxy Bacillus is also higher.Such as table 4.Looked into newly with reference to Patents,
It has selected Osthole and carry out compounding research and development.
Effect test 5：2-cyano-3-amino-3-phenylancryic acetate and extensive screening of the Osthole to fusarium graminearum sensitive strain；
Strains tested：Wheat scab（Gibbrella zeae）, sensitive strain G4, the preservation of this laboratory cultures.
Test method：Reference test 1, in vitro Plating.Compounded combination synergistic effect statistical method is as follows：Single dose
It is respectively P to survey inhibiting ratea、Pb, then theoretical death rate P=1- after same dose is mixed（1-Pa）（1-Pb）, synergistic effect
Z=actual measurement inhibiting rate-theory inhibiting rate evaluation criterion：Z<- 10 be antagonism, -10< Z <10 is are added, Z> 10
For synergy.
Result of the test：When 2-cyano-3-amino-3-phenylancryic acetate and Osthole proportioning are 12.8 ~ 0.004：When 1, each combination show to be added or
Synergistic effect, when 2-cyano-3-amino-3-phenylancryic acetate be 0.1,0.2,0.4,0.8 part, when Osthole is 1 part, synergistic effect is more than 10, shows
Synergy trend, other combinations show addition trend.Such as table 5.
Effect test 6：2-cyano-3-amino-3-phenylancryic acetate is screened with Osthole to the synergy of fusarium graminearum sensitive strain
Strains tested：Wheat scab（Gibbrella zeae）, sensitive strain G4, the preservation of this laboratory cultures.
Test method：Reference test 1, in vitro Plating.Synergy evaluation uses co-toxicity coefficient method.
Result of the test：When 2-cyano-3-amino-3-phenylancryic acetate and Osthole proportioning are 10~0.1：When 1, each combination shows to be added or synergy
Effect, is matched as 10：1 or 1：Synergistic effect is obvious when 1.Such as table 6.
Effect test 7：2-cyano-3-amino-3-phenylancryic acetate and synergistic function of the Osthole to wheat powdery mildew
Test material：Wheat powdery mildew [Erysiphe graminis D.c.f.sp.tritici E.Marchal],
Indoor living parasitism culture.
Test method：The 4th, reference NY/T1156.4-2006 farm-chemical indoor determination test rules bactericide
Point：Prevent and treat 24h after wheat powdery mildew experiment pot-culture method, spray and connect bacterium, test protective effect.
Result of the test：Result is investigated using the method for range estimation classification, concrete outcome such as table 7.
As a result show：Osthole for preventing and treating wheat powdery mildew efficiently, dosage be 1,10,100 ug/ml when spraying after 8 days prevent
Effect is respectively 40%, 60%, 90%.2-cyano-3-amino-3-phenylancryic acetate preventing and treating wheat powdery mildew preventive effect it is relatively low, dosage be 1,10,100ug/ml when preventive effect
Respectively 15%, 30%, 50%.2-cyano-3-amino-3-phenylancryic acetate is 10 with Osthole proportioning：1、5：1、1：1、0.2：1、0.1：Synergistic effect when 1
For 2 ~ 12, show to be added or synergistic effect.
It should be noted that it is above-mentioned be only presently preferred embodiments of the present invention, not for limit the present invention protection model
Enclose, made equivalents belong to protection scope of the present invention on the basis of above-described embodiment.
Claims (3)
1. a kind of bactericidal composition of 2- cyano group -3- amino -3- ethyl phenylacrylates and Osthole, it is characterised in that institute
Active component includes in the bactericidal composition stated：
Component A：2- cyano group -3- amino -3- ethyl phenylacrylates；
B component：Osthole；
Wherein, the weight ratio of component A and B component is 0.1 in described bactericidal composition:1,0.2:1,0.4:1,0.8:1,1:1
With 10:1.
2. the bactericidal composition of 2- cyano group -3- amino -3- ethyl phenylacrylates as claimed in claim 1 and Osthole,
Characterized in that, the water dispersible granules, suspending agent, aqueous emulsion, wettable that described bactericidal composition can be configured to agriculturally to allow
Property pulvis and missible oil.
3. the Bactericidal composite of 2- cyano group -3- amino -3- ethyl phenylacrylates as claimed in claim 1 or 2 and Osthole
Thing, it is characterised in that described bactericidal composition can be used for administering wheat scab, wheat powdery mildew, take-all, paddy rice
Bakanae disease, rice sheath blight disease and rice green smut.
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