CN108041063A - A kind of bactericidal composition of containing cymoxanil and its application - Google Patents
A kind of bactericidal composition of containing cymoxanil and its application Download PDFInfo
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- CN108041063A CN108041063A CN201711496867.0A CN201711496867A CN108041063A CN 108041063 A CN108041063 A CN 108041063A CN 201711496867 A CN201711496867 A CN 201711496867A CN 108041063 A CN108041063 A CN 108041063A
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- Prior art keywords
- cymoxanil
- downy mildew
- preparation
- azoles ether
- cucumber downy
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to technical field of pesticide, are related to a kind of composition pesticide, it is characterised in that active ingredient naphthalene azoles ether sulfanilamide (SN) and cymoxanil.Said composition can be configured to aqueous emulsion, suspending agent, water dispersible granules, and for preventing cucumber downy mildew, synergistic effect is notable, extend the lasting period, environmentally friendly.
Description
Technical field
The present invention relates to disinfectant use in agriculture fields, specifically, are related to a kind of microbicide compositions and its application, particularly
It is related to a kind of bactericidal composition and its application for being used to prevent cucumber downy mildew.
Background technology
Naphthalene azoles ether sulfanilamide (SN) is in 2016, found by Central China Normal University (Chinese patent, CN201610003201,
CN201610095896), there is preferable control effect to cucumber downy mildew, rice sheath blight disease.Following (I) institute of its chemical constitution
Show.
Cymoxanil is a kind of efficient, less toxic fungicide, effective to the white category of Peronosporales fungi such as epidemic disease, Peronospora, uniaxial frost category.
Cymoxanil is mixed with other protective fungicides extensively, is used in the brassicaceous vegetables such as cucumber, grape, tomato, lichee, huge dish
And tobacco etc..
Cucumber downy mildew is the more serious disease of cucumber, all can be aggrieved from seedling stage to strain, mainly cause harm blade and stem.
The disease is 16 DEG C~24 DEG C suitable for onset temperature, and irregular chlorisis yellowish-brown spot occurs for Seedling Stage morbidity, cotyledon front, into
Strain morbidity, mostly since greenhouse forward position, there is water stain shape, light green fleck, front in blade reverse side in middle and lower part before this for morbidity strain
It does not show, rear scab gradually expands, front exposed.It is badly in need of currently on the market good to the quick-acting of cucumber downy mildew, lasting period length
Medicament.
The content of the invention
The object of the present invention is to provide a kind of composition pesticide with synergistic effect and safety and environmental protection, for preventing cucumber
Downy mildew compared with existing pesticide control, realizes compound synergic, dosage is reduced, so as to achieve the purpose that reduce cost.Specifically
Be related to naphthalene azoles ether sulfanilamide (SN) and the bactericidal composition of cymoxanil.
The present inventor has found by in-depth study, and the naphthalene azoles ether sulfanilamide (SN) of following specific structures is answered with cymoxanil
With use, not only without offsetting naphthalene azoles ether sulfanilamide (SN) and the respective drug effect of cymoxanil, on the contrary, also making with obviously synergy
With significantly improving to the control effect of fungus-caused plant disease, particularly the control effect of cucumber downy mildew significantly carried
Height has thus completed the present invention.
To achieve these goals, the present invention provides a kind of bactericidal composition, wherein, the bactericidal composition activity into
Dividing includes naphthalene azoles ether sulfanilamide (SN) and cymoxanil, and the naphthalene azoles ether sulfanilamide (SN) is formula (I) compound,
The present invention provides application of the above-mentioned microbicide compositions in cucumber downy mildew is prevented.
The present invention provides a kind of preparation as prepared by above-mentioned microbicide compositions, wherein, the dosage form of the preparation is
Aqueous emulsion, suspension emulsion or water dispersible granules.
The present invention provides application of the above-mentioned preparation in cucumber downy mildew is prevented.
The microbicide compositions provided according to the present invention, with apparent synergistic function, bactericidal effect is good, medication
It is at low cost, it is not easy to produce resistance, it is good to crop safety.Microbicide compositions particularly provided by the invention are to cucumber downy mildew
Control effect is notable.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Specific embodiment
To determine the synergistic effect of naphthalene azoles ether sulfanilamide (SN) and cymoxanil two kinds of active ingredients compounding, applicant first using this two
Kind ingredient carries out indoor biometrics experiment, and after the action character of two kinds of active ingredient is analyzed, the raw object surveyed is set to by we
Cuba artificial downy mildew (Pseudoperonospora cubensis), the Pseudomonas Mastigomycotina fungi, can cause cucumber
Downy mildew.
The test method of indoor biometrics：Reference standard NY/T1156.7-2006 measures medicament to Huang using growth rate method
The inhibitory action of melon downy mildew pathogen mycelial growth.On the basis of pilot study, germ mycelial growth is pressed down from each medicament
Naphthalene azoles ether sulfanilamide (SN) and cymoxanil active compound are first made by rate processed up to 5 concentration of design in the range of 10%~90% by solvent of acetone
The liquid of series concentration is spare, and liquid then is added to own thawing in 1% ratio and is cooled in the PDA culture medium of room temperature,
It after fully shaking up, pours into the culture dish (diameter 90mm) of sterilizing and band medicine tablet is made, often handle four repetitions, it is sterile to add in
The processing of water is blank control；With card punch (diameter 5mm) picking bacterium of downy mildew of cucumber bacteria cake of sterilizing, mycelia is sterile down
The center with medicine tablet is inoculated in, is inverted in culture in 25 DEG C of constant incubator, is measured after cultivating 6d using crossing method
Colony diameter calculates EC of each medicament to pathogen50, and toxicity index is calculated according to the following equation, compare different agents
Virulence and bacteriostasis.Active compound used in above-mentioned raw test and reagent are bought from other producers.
Drug effect calculates:Two diameters are surveyed in each bacterium colony right-angled intersection, and bacterium colony size is represented with its average.It is obtained as the following formula
Colony growth inhibiting rate:Colony growth inhibiting rate %=(blank control bacterium colony increases diameter-chemicals treatment bacterium colony and increases diameter)
X100/ blank controls bacterium colony increases diameter.
As a result data analysis statistical is carried out with 3.11 professional versions of DPS, regression straight line, EC is obtained50, related coefficient.According to grandson
((Y-P Sun) method is by the EC respectively handled of measure by Yun Pei50It is converted into actual poison exponent (ATI)；According to the proportioning of mixture, obtain
Theoretical toxicity index (TTI) is obtained, the co-toxicity coefficient (CTC) of mixture is calculated by following equation.
Toxicity index (TI)=(EC of standard agent50The EC of/reagent agent50) X 100 is (a kind of work in A agent, B agent
For standard agent)
The actual toxicity index of mixture (ATI)=(EC of standard agent50The EC of/mixture50)X 100
There is the TI X mixtures of the content+B agent of A in the TI X mixture active ingredients of mixture theoretical toxicity index (TTI)=A agent
Imitate the content of B in ingredient
Co-toxicity coefficient (CTC)=[the actual toxicity index of mixture ((ATI)/mixture theoretical toxicity index (TTI) " X 100
A agent, B agent are respectively two kinds of active ingredients mutually mixed, and mixture is the mixture after A agent is mutually mixed with B agent.
Evaluation criterion:When CTC >=120, synergistic effect is shown as；When CTC≤80, antagonism is shown as；80 ＜ CTC ＜
120, show as summation action.
1 formula of table (I) or (II) compound compound the indoor virulence to cucumber downy mildew with cymoxanil
| Processing | EC50(mg/L) | TTI | ATI | Co-toxicity coefficient (CTC) |
| Naphthalene azoles ether sulfanilamide (SN) (A) | 0.0065 | |||
| Cymoxanil (B) | 1.0458 | |||
| A+B(20:1) | 0.0051 | 95.27 | 127.45 | 133.78 |
| A+B(10:1) | 0.0041 | 90.96 | 158.54 | 174.28 |
| A+B(5:1) | 0.0032 | 83.44 | 203.13 | 243.45 |
| A+B(1:1) | 0.0046 | 50.31 | 141.30 | 280.86 |
| A+B(1:5) | 0.020 | 17.18 | 32.50 | 189.12 |
| A+B(1:10) | 0.045 | 9.66 | 14.44 | 149.59 |
| A+B(1:20) | 0.092 | 5.35 | 7.07 | 131.97 |
Remarks:The numeric ratio in bracket that W is represented is the weight proportion of two kinds of medicaments.
Above-mentioned data are analyzed, it is apparent that when naphthalene azoles ether sulfanilamide (SN) and cymoxanil compound in varing proportions, formula
Co-toxicity coefficient (CTC) is all higher than 120, the synergistic effect for having different proportion to the prevention and control capability of cucumber downy mildew.
It is tested by indoor biometrics, after determining two kinds of active ingredient compoundings of naphthalene azoles ether sulfanilamide (SN) and cymoxanil, can had very well
Synergistic effect.Below by way of specific example to be further described the present invention, but the present invention be only limited to absolutely not it is following
These embodiments.Percentage is weight percentage in formula, and used active compound and auxiliary agent are the purchase of other producers.
Preparation example 1：The preparation of 40% naphthalene azoles ether sulfanilamide (SN)-cymoxanil suspending agent G1
15% naphthalene azoles ether sulfanilamide (SN), 25% cymoxanil are weighed, 2%TERSPERSE 2500 (is purchased from Hensel Mann, following phase
Together), 3%TERSPERSE 4896 (being purchased from Hensel Mann, same as below), 0.2% xanthans, 3% white carbon, 5% second two
Alcohol, 0.3% benzoic acid, 0.5% organic silicon defoamer (trade name:S-29 Nanjing "four news" (new ideas applied chemistry product company produces, following phase
Together), deionized water adds to 100%.
Preparation method:Active ingredient, wetting agent, dispersant, antifreezing agent etc. are uniformly mixed, adds in sand mill and is ground to
After certain grain size, filtering.It adds prepared xanthans and is stirred until homogeneous i.e. available 40% naphthalene azoles ether sulfanilamide (SN)-cymoxanil and hang
Floating agent G1.
Preparation example 2：The preparation of 15% naphthalene azoles ether sulfanilamide (SN)-cymoxanil aqueous emulsion G2
Weigh 10% naphthalene azoles ether sulfanilamide (SN), 5% cymoxanil, 3% agriculture breast 2201#, 1.5% Nonyl pheno (EO=10)
Ether phosphate (is purchased from Nanjing Teva-Chem. Co., Ltd., same as below), 2.5% triphenoethyl benzene phenol polyethenoxy ether phosphate
(agriculture breast 600# acid phosphatide, is purchased from Nanjing Teva-Chem. Co., Ltd., same as below), 2%Span-60# (are purchased from Xingtai blue star to help
Agent company, same as below), 1% epoxychloropropane, 20% dimethylbenzene, 10% cyclohexanone, 0.3% xanthans, 0.5% benzoic acid,
Deionized water adds to 100% mass parts.
Preparation method：By above-mentioned active ingredient, emulsifier after dispersant is dissolved with solvent, under conditions of high shear, adds
Enter into water phase, add xanthans and stir evenly, you can 15% naphthalene azoles ether sulfanilamide (SN)-cymoxanil aqueous emulsion G2 is made.
Preparation example 3：The preparation of 60% naphthalene azoles ether sulfanilamide (SN)-cymoxanil water dispersible granules G3
20% naphthalene azoles ether sulfanilamide (SN) is weighed, 40% cymoxanil, 3%TERSPERSE 2700 (are purchased from Hensel Mann, following phase
Together), 2% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products are purchased from Shanghai Tiantan Accessory Ingredient Co., Ltd, same as below),
3% Nekal BX (nekal), 2%K-12 lauryl sodium sulfate), 3% diatomite, 5% glucose, kaolin
Add to 100%.
Preparation method:60% naphthalene azoles ether sulphur is made in above-mentioned raw materials after mixing, ultra micro air-flow crushing, granulation step
Amine-cymoxanil water dispersible granules G3.
Preparation example 4：The preparation of 24% naphthalene azoles ether sulfanilamide (SN) suspending agent
Weigh 24% naphthalene azoles ether sulfanilamide (SN), 2425,0.2% xanthans of 2%TERSPERSE 2500,3%TERSPERSE,
3% white carbon, 5% ethylene glycol, 0.3% benzoic acid, 0.5% organic silicon defoamer (trade name:S-29 Nanjing "four news" (new ideas applied chemistry
Product company produces), deionized water adds to 100%.
Preparation method:Active ingredient, wetting agent, dispersant, antifreezing agent etc. are uniformly mixed, adds in sand mill and is ground to
After certain grain size, filtering.It adds prepared xanthans and is stirred until homogeneous and can be prepared by 24% naphthalene azoles ether sulfanilamide (SN) suspending agent.
Embodiment：This test case is used to illustrate to prevent cucumber downy mildew field control effectiveness test
Selection above example carries out the field trial of prevention cucumber downy mildew, determines the effect of formula prevention cucumber downy mildew
Fruit, at the same with naphthalene azoles ether sulfanilamide (SN) and the Contrast on effect of cymoxanil single dose, verify the control effect of the embodiment of the present invention.Specific experiment
Method is with reference to GB/T17980.26-2000.Test site is Chengdu, Sichuan, and the time is on April 8th, 2017, and experiment is each
Four repetitions, cell random alignment are handled, each plot area is 30m2, using back-pack electric compression sprayer, dispenser is worked as
It is sunny calm, and the planting habit of all cells is identical with local planting habit, records dispenser, specific as follows:
Investigation method is:Take 4 points of investigation at random per cell, look into two plants, the whole blades of every plant of investigation at every.
The grade scale of disease index:
0 grade:Disease-free spot；
1 grade:Lesion area accounts for less than the 5% of entire leaf area；
3 grades:Lesion area accounts for the 6%-l0% of entire leaf area；
5 grades:Lesion area accounts for the 11%-25% of entire leaf area；
7 grades:Lesion area accounts for the 26%-50% of entire leaf area；
9 grades:Lesion area accounts for more than the 51% of entire leaf area.
Drug effect computational methods:According to investigation result, disease index and preventive effect are calculated according to formula below.
In addition, dispenser drug, active ingredient amount and test result are as shown in table 2
2 various medicaments of table are to the field control effectiveness test of cucumber downy mildew
Can be seen that bactericidal composition provided by the invention from more than effect data has spy for prevention cucumber downy mildew
Effect, component is reasonable, has treatment plus protective effect, and bactericidal effect is good, thus, it is possible to reduce times for spraying, reduce medication into
This.More importantly its activity and bactericidal effect are not the simple superposition of each component activity, but have significant synergies,
The generation of resistance can be slowed down, to crop safety, meet the security requirement of pesticidal preparations.
As it can be seen that complex preparation can be very good to improve drug effect, extend the lasting period, mitigate the amount of labour of peasant, have very high
Practicability.
Claims (4)
1. a kind of bactericidal composition, which is characterized in that the active ingredient of the bactericidal composition includes naphthalene azoles ether sulfanilamide (SN) and cymoxanil,
The naphthalene azoles ether sulfanilamide (SN) is following formula (I) compounds,
Wherein, the weight ratio of naphthalene azoles ether sulfanilamide (SN) and cymoxanil is 1:20~20:1.
2. application of the microbicide compositions described in claim 1 in cucumber downy mildew is prevented.
3. the preparation prepared by microbicide compositions described in claim 1, wherein, the dosage form of the preparation is aqueous emulsion, hangs
Emulsion or water dispersible granules.
4. application of the preparation described in claim 3 in cucumber downy mildew is prevented.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711496867.0A CN108041063A (en) | 2017-12-31 | 2017-12-31 | A kind of bactericidal composition of containing cymoxanil and its application |
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| CN201711496867.0A CN108041063A (en) | 2017-12-31 | 2017-12-31 | A kind of bactericidal composition of containing cymoxanil and its application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110999916A (en) * | 2019-12-27 | 2020-04-14 | 利民化学有限责任公司 | Compound bactericide and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101601401A (en) * | 2009-03-26 | 2009-12-16 | 陕西蒲城县美邦农药有限责任公司 | A kind of bactericidal composition that contains dithianon and white urea cyanogen |
| CN105693638A (en) * | 2016-01-05 | 2016-06-22 | 华中师范大学 | Bactericidal compound and bactericide composition and preparation and application thereof |
-
2017
- 2017-12-31 CN CN201711496867.0A patent/CN108041063A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101601401A (en) * | 2009-03-26 | 2009-12-16 | 陕西蒲城县美邦农药有限责任公司 | A kind of bactericidal composition that contains dithianon and white urea cyanogen |
| CN105693638A (en) * | 2016-01-05 | 2016-06-22 | 华中师范大学 | Bactericidal compound and bactericide composition and preparation and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110999916A (en) * | 2019-12-27 | 2020-04-14 | 利民化学有限责任公司 | Compound bactericide and application thereof |
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