CN103988847A - Bactericidal composition containing chitosan oligosaccharide and methylene dithiocyanate - Google Patents
Bactericidal composition containing chitosan oligosaccharide and methylene dithiocyanate Download PDFInfo
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- CN103988847A CN103988847A CN201410249256.6A CN201410249256A CN103988847A CN 103988847 A CN103988847 A CN 103988847A CN 201410249256 A CN201410249256 A CN 201410249256A CN 103988847 A CN103988847 A CN 103988847A
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the field of plant protection and in particular relates to a bactericidal composition containing chitosan oligosaccharide and methylene dithiocyanate. In the composition, a mass ratio of the bactericidal composition containing chitosan oligosaccharide and methylene dithiocyanate to chitosan oligosaccharide is 40:1-1:40; and the mass sum of the chitosan oligosaccharide and methylene dithiocyanate accounts for 0.1-80 percent of total mass of the composition. The composition is used for controlling nematode, wilt, fusarium moniliforme and pepper bacterial wilt.
Description
Technical field
The invention belongs to plant protection field, particularly relate to the bactericidal composition and the purposes that contain chitosan oligosaccharide and dithiocyano-methane.
Background technology
Along with the variation of cropping system, generation and the harm of plant disease are on the rise, and cause of disease also improves the pesticide resistance of bactericide thereupon, and exploring agricultural chemicals efficient, low toxicity becomes one of hot issue of related scientific research and management work person's concern.Chitosan oligosaccharide, resisting in the process of pathogen erosion, can be induced and excite plant to produce a series of defense reaction, and these defense reactions synergies stop propagation and the invasion of pathogens, reach the effect of preventing and curing diseases.Dithiocyano-methane (MBT) is that one is efficiently killed algae sterilization chemicals, MBT has efficient killing effect for the main bacteria existing in cycle water, fungi and algae (all kinds of fungi), and duration of efficacy is long, the pH value of adaptation and wider range.The consumption of use 3-4PPM up to more than 99%, also can reach high sterilizing rate to other all kinds of bacterium to the sterilizing rate of heterotroph, nitrite bacteria, sulfosalt reducing bacteria, Escherichia coli, iron wire bacterium, anaerobic bacteria, denitrifying bacterium and sulphur bacteria.In all kinds of fungies of removing, dithiocyano-methane is than the better effects if of its homicide/degerming medicine.Its stoste is stable, in the time of application, can degrade rapidly or decompose again, can not cause secondary pollution, highly-effective low-residue to environment.Bakanae disease of rice and Aplelenchiodes bessegi of rice, stripe disease of barley, net blotch, covered smut etc. are had to higher preventive effect.Kind of a biography nematode, bacterium and caused other disease of fungi are also had to good preventive effect simultaneously, under recommended dose, to rice Wheat Seeds safety, and have certain effect to improving Germination Percentage of Rice and percentage establishment of seedling.
Inventor has carried out large quantity research to the composition that contains chitosan oligosaccharide and dithiocyano-methane, has found the effect that some are unexpected, so completed the present invention.
Summary of the invention
The object of this invention is to provide bactericidal composition that contains chitosan oligosaccharide and dithiocyano-methane having synergistic effect and uses thereof.
Concrete, the invention provides the bactericidal composition that contains chitosan oligosaccharide and dithiocyano-methane, wherein the mass ratio of chitosan oligosaccharide and dithiocyano-methane is 40:1-1:40, preferred, mass ratio is 10:1-1:10.
In above-mentioned composition, the percentage that the quality summation of chitosan oligosaccharide and dithiocyano-methane accounts for composition gross mass is 0.1-80%.
Above-mentioned composition, is characterized in that composition is with seed dressing, spraying, filling root, immersion applications.
The present invention also provides above-mentioned composition for preventing and treating the purposes of crop pest, and wherein said crop is cereal crops, vegetables, forest or other crops, and cereal crops are paddy rice, peanut, corn, soybean, potato, Chinese sorghum or wheat; Vegetables are cucumber, capsicum, tomato, celery, spinach, Chinese cabbage or beet; Forest is willow, pine tree, cypress, cocoanut tree or bamboo grows, and other crops are banana, watermelon, apple, pear tree, tobacco or oranges and tangerines.
In such use, the disease of suitable control comprises: nemas, virus disease, the fungus-caused fusarium wilt of Deuteromycotina, gray mold etc., the fungus-caused bakanae disease of Ascomycotina, powdery mildew, stalk break etc., banded sclerotial blight that Basidiomycotina causes, rust, smut etc., the fungus-caused downy mildew of Mastigomycotina, late blight etc.
The purposes of foregoing, preferred, disease is banana root-knot nematode, cucumber root-knot nematode, watermelon blight, bakanae disease of rice and banded sclerotial blight, pepper virus disease, Botrytis cinerea and pepper ralstonia solanacearum etc.
Beneficial effect of the present invention is:
1, synergistic effect is obvious: composition shows good synergistic effect in certain ratio range, has improved the protection effect of composition.
2, expanding prevention spectrum: composition can be prevented and treated the multiple diseases in agricultural production.
3, reduce use cost: because compositely thering is the preventive effect of raising, control spectrum increases, and has reduced access times, has reduced use cost;
4, delay pesticide resistance: the composition of two kinds of different mechanism of action is composite, pathogen is difficult for developing immunity to drugs, and delays pathogen to the drug-fast generation of single dose.
Embodiment
The following stated, only for explaining the present invention, for not departing from amendment, replacement or the improvement that the present invention is spiritual and principle is made, all belongs to the scope of protection of present invention.
The effect of the composition of embodiment 1 chitosan oligosaccharide and dithiocyano-methane to banana root-knot nematode
The composition of dithiocyano-methane single dose, chitosan oligosaccharide single dose and certain mass ratio is diluted to certain variable concentrations.The quantitative dilution that contains banana root-knot nematode is dripped on slide to microscopy counting; Microscopy is sneaked into the root-knot nematode dilution of quantitative pharmacy in the same way, and microscopy counting, measures respectively each reagent agent lethal concentration of 50 LC
50, according to the co-toxicity coefficient of the abundant method calculation composition of Sun Yun (CTC value), CTC value be greater than 120 for synergistic effect, concrete grammar is as follows.
Setting a kind of single dose (A) is standard single dose, and specifying its toxicity index (TI) is 100:
The percentage of the TI × single dose of theoretical toxicity index (the TTI)=each single dose of Σ of mixture in mixture
Result is as following table 1.
The synergistic effect of the composition of table 1 chitosan oligosaccharide and dithiocyano-methane to banana root-knot nematode
As can be seen from Table 1, in the time that the proportioning of chitosan oligosaccharide and dithiocyano-methane is 40:1-1:40, composition is all greater than 120 to the co-toxicity coefficient of banana root-knot nematode, illustrates that the composition of chitosan oligosaccharide and dithiocyano-methane is obvious to the synergistic effect of banana root-knot nematode.
The preventive effect of the composition of embodiment 2 chitosan oligosaccharides and dithiocyano-methane to cucumber nematode
Be mixed into compost with sterilizing is native with sterilizing river sand, sneak into composition or single dose, fully mix thoroughly, add clear water to make water content 30%, pack basin alms bowl into, have the cucumber seedling of 2 true leaves to be transplanted in basin growth, transplant latter 7 days, 200 of every basin access Meloidogyne incognita 2 instar larvaes, are placed in greenhouse, quantitative watering management.If 3 repetitions of every processing, and establish that not sneak into chemicals treatment be blank.After 20 days, in the time that blank plant rhizosphere has a large amount of root knot, investigation plant height and preventive effect.Record every strain root knot number, and carry out classification by Liu Weizhi (1995): 0 grade, without root knot; 1 grade, 1-2 root knot; 2 grades, 3-10 root knot; 3 grades, 11-30 root knot; 4 grades, 31-100 root knot; 5 grades, more than 100 root knot.The results are shown in Table 2.
The effect of the composition of table 2 chitosan oligosaccharide and dithiocyano-methane to cucumber root-knot nematode
Table 2 shows, with respect to corresponding single dose and blank, the processing plant height of the composition of chitosan oligosaccharide and dithiocyano-methane obviously improves, and root knot number obviously reduces, and disease obviously alleviates.Illustrate that composition is obvious to the control efficiency of cucumber root-knot nematode, promote plant growth.
The effect of the composition of embodiment 3 chitosan oligosaccharides and dithiocyano-methane to watermelon blight
According to scheme, preparation built agent and single dose preparation, and carry out active preliminary experiment.According to trial test, preparation is mixed with to the concentration needing.Before selecting, stubble watermelon blight morbidity field, arranges field evenly.Evenly dress seed (concentration is in effective milligram quantities of every kilogram of seed with medicament), sow Routine Management with the suspension seed-coating agent of different medicaments.In the time of disease onset, investigate watermelon plant diseases situation.Use Abbot formula, convert percentage to preventive effect.
In theoretical effect, x is the effect of the substance A of concentration while being a, and y is the effect of the substance B of concentration while being b, and relatively theoretical effect and actual effect evaluated the synergy of composition, while being greater than theoretical preventive effect taking actual preventive effect as synergistic effect.Composition the results are shown in Table 3 to watermelon disease.
The effect of the composition of table 3 chitosan oligosaccharide and dithiocyano-methane to watermelon blight
Test by composition to watermelon blight, as seen from Table 3, the composition of chitosan oligosaccharide and dithiocyano-methane is the theoretical preventive effect higher than correspondent composition to the effect of watermelon blight.Obviously, the composition of chitosan oligosaccharide and dithiocyano-methane is synergy to watermelon blight.
The effect of the composition of embodiment 4 chitosan oligosaccharides and dithiocyano-methane to rice disease
According to scheme, preparation built agent and single dose preparation, and carry out active preliminary experiment.According to determination of activity trial test, preparation is mixed with to the concentration needing.Select once to fall ill rice field, carry out paddy rice seed dressing (concentration is in effective milligram quantities of every kilogram of seed with medicament), sowing, seedling.In the time that blank bakanae disease of rice, banded sclerotial blight evil occur, institute an inquiry, every processing is by 5 samplings, and every 50 strains, measure the percentage that infect on plant blade face.Use Abbot formula, convert percentage to preventive effect.
In theoretical effect, x is the effect of the substance A of concentration while being a, and y is the effect of the substance B of concentration while being b, and relatively theoretical effect and actual effect evaluated the synergy of composition, while being greater than theoretical preventive effect taking actual preventive effect as synergistic effect.Composition the results are shown in Table 4 to rice plant disease.
The field effect of the composition of table 4 chitosan oligosaccharide and dithiocyano-methane to rice disease
As seen from Table 4, the composition of chitosan oligosaccharide and dithiocyano-methane in the time of 1:1 and 4:1, composition to the actual effect of bakanae disease of rice, rice sheath blight disease all higher than the theoretical preventive effect of composition.Obviously, the composition of chitosan oligosaccharide and dithiocyano-methane is synergy to bakanae disease of rice, rice sheath blight disease.
The effect of the composition of embodiment 5 chitosan oligosaccharides and dithiocyano-methane to pepper diseases
According to scheme, preparation built agent and single dose preparation, and carry out active preliminary experiment.According to determination of activity trial test, preparation is mixed with to the concentration needing.Before test, establish corresponding single dose and blank in capsicum field, adopt the conventional conventional spray of manual sprayer, every medicament sprays 50 strains.7-10d dispenser 1 time again after the 1st dispenser.10-20d after the 2nd dispenser, investigates the pepper plant disease situation of listing.Use Abbot formula, convert percentage to preventive effect.
In theoretical effect, x is the effect of the substance A of concentration while being a, and y is the effect of the substance B of concentration while being b, and relatively theoretical effect and actual effect evaluated the synergy of composition, while being greater than theoretical preventive effect taking actual preventive effect as synergistic effect.Composition to the result of pepper plant disease respectively in table 5.
The field effect of the composition of table 5 chitosan oligosaccharide and dithiocyano-methane to pepper diseases
As seen from Table 5, the composition that contains chitosan oligosaccharide and dithiocyano-methane all higher than theoretical effect, shows that composition has obvious synergistic function to the actual effect of pepper virus disease, gray mold and bacterial wilt.
Claims (7)
1. contain the bactericidal composition of chitosan oligosaccharide and dithiocyano-methane, the mass ratio that it is characterized in that chitosan oligosaccharide and dithiocyano-methane is 40:1-1:40.
2. composition according to claim 1, is characterized in that chitosan oligosaccharide and dithiocyano-methane mass ratio are 10:1-1:10.
3. composition according to claim 2, the percentage that the quality summation that it is characterized in that chitosan oligosaccharide and dithiocyano-methane accounts for composition gross mass is 0.1-80%.
4. composition according to claim 3, is characterized in that composition is with seed dressing, spraying, filling root, immersion applications.
5. composition claimed in claim 4 is for preventing and treating the purposes of crop pest, and wherein said crop is cereal crops, vegetables, forest or other crops, and cereal crops are paddy rice, peanut, corn, soybean, potato, Chinese sorghum or wheat; Vegetables are cucumber, capsicum, tomato, celery, spinach, Chinese cabbage or beet; Forest is willow, pine tree, cypress, cocoanut tree or bamboo grows, and other crops are banana, watermelon, apple, pear tree, tobacco or oranges and tangerines.
6. purposes according to claim 5, the disease of suitable control comprises: nemas, virus disease, the fungus-caused fusarium wilt of Deuteromycotina, gray mold etc., the fungus-caused bakanae disease of Ascomycotina, powdery mildew, stalk break etc., banded sclerotial blight that Basidiomycotina causes, rust, smut etc., the fungus-caused downy mildew of Mastigomycotina, late blight etc.
7. purposes according to claim 6, disease is banana root-knot nematode, cucumber root-knot nematode, watermelon blight, bakanae disease of rice and banded sclerotial blight, pepper virus disease, Botrytis cinerea and pepper ralstonia solanacearum.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106305759A (en) * | 2016-08-22 | 2017-01-11 | 田文华 | Pesticide composition containing fluorine thiamethoxam sulfone and dithiocyano methane |
| CN110946149A (en) * | 2019-12-09 | 2020-04-03 | 王映镝 | Bactericidal and algae-killing synergistic composition for paint, coating, water body and wood and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1210674A (en) * | 1998-07-28 | 1999-03-17 | 南京农业大学 | Method for preventing and controlling plant diseases in agriculture |
| CN101637177A (en) * | 2009-09-02 | 2010-02-03 | 云南省农业科学院农业环境资源研究所 | Preventing agent of erigeron breviscapus root rots and application thereof |
| CN102138554A (en) * | 2010-02-02 | 2011-08-03 | 夏正斌 | Method for preparing methylene dithiocyanate emulsion serving as efficient bactericide |
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2014
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Patent Citations (3)
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| CN1210674A (en) * | 1998-07-28 | 1999-03-17 | 南京农业大学 | Method for preventing and controlling plant diseases in agriculture |
| CN101637177A (en) * | 2009-09-02 | 2010-02-03 | 云南省农业科学院农业环境资源研究所 | Preventing agent of erigeron breviscapus root rots and application thereof |
| CN102138554A (en) * | 2010-02-02 | 2011-08-03 | 夏正斌 | Method for preparing methylene dithiocyanate emulsion serving as efficient bactericide |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106305759A (en) * | 2016-08-22 | 2017-01-11 | 田文华 | Pesticide composition containing fluorine thiamethoxam sulfone and dithiocyano methane |
| CN110946149A (en) * | 2019-12-09 | 2020-04-03 | 王映镝 | Bactericidal and algae-killing synergistic composition for paint, coating, water body and wood and application thereof |
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| CN103988847B (en) | 2015-12-09 |
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