CN104542638A - Plant source preparation for preventing and controlling agrobacterium tumefaciens conn and preparation method of plant source preparation - Google Patents
Plant source preparation for preventing and controlling agrobacterium tumefaciens conn and preparation method of plant source preparation Download PDFInfo
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- CN104542638A CN104542638A CN201410558083.6A CN201410558083A CN104542638A CN 104542638 A CN104542638 A CN 104542638A CN 201410558083 A CN201410558083 A CN 201410558083A CN 104542638 A CN104542638 A CN 104542638A
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Abstract
The invention discloses a plant source preparation for preventing and controlling agrobacterium tumefaciens conn and a preparation method of the plant source preparation. The method comprises the following steps: mixing hairyvein agrimony herb, jasminum grandiflorum and oldenlandia diffusa herb in the weight ratio of 1 to 1 to 1; crushing the mixture into particles with a crusher; screening through a 60mesh sieve, putting 20kg of particles into an extraction kettle of a traditional Chinese medicine multifunctional extraction equipment to extract; adding 75L of 75% ethanol, stewing at 80-84 DEG C for 2.0 hours, concentrating for an hour to prepare a compound extract concentrated paste and crystal of the hairyvein agrimony herb, the jasminum grandiflorum and the oldenlandia diffusa herb; and adding a 75% ethanol solvent, fully dissolving to prepare a saturated liquid, bottling and sealing to prepare the preparation. An experiment result shows that the plant source preparation for preventing and controlling the agrobacterium tumefaciens conn has relatively high prevention and treatment effects on the agrobacterium tumefaciens conn, and has the advantages of no environmental pollution, low cost, simple preparation process, safety, directness, environmental friendliness and high efficiency; and the cost is saved.
Description
Technical field
The present invention relates to forestry pest control field, be specially a kind of plant resource preparation preventing and treating fruit root knot disease.
Background technology
Fruit root knot disease is a kind of disease common on fruit tree, especially occurs more with nursery, the multiple fruit trees such as apple, pears, peach, apricot, Lee of can causing harm.This disease mainly occurs in fruit tree collar portion, and other position of root also can occur.Their early stage forms linen knob, and interior tissue is soft, and rough surface is uneven.Along with knurl body constantly increases, surface crossfades into brown, and epidermal cell is withered, interior tissue suberification, causes the fruit tree significantly underproduction, develops into the later stage and causes tree body dead.This disease is listed in international great disease, can deserve to be called the cancer in fruit diseases, day by day come into one's own.
The cause of disease of fruit root knot disease is Agrobacterium tumefaciens
Agrobacterium tumemfaciens(Smith etTowns) Conn.agrobacterium tumefaciens, survive the winter in the cortex of cancer tumor tissue, or when cancer knurl breaks decortication, enter in soil and survive the winter.Germ invades host by wound, can cause host cell abnormal division, forms cancer knurl.The time manifested needed for disease knurl is invaded, generally by several thoughtful more than 1 year from germ.Therefore, can kill Agrobacterium tumefaciens from mechanism screening, the preparation suppressing host cell abnormal division will be a kind of research method of shooting the arrow at the target.We think and develop and can kill Agrobacterium tumefaciens from new plant resource secondary metabolite, suppress the eco-friendly plant resource preparation control fruit root knot that is novel, efficient, nontoxic, noresidue of host cell abnormal division sick, there is wide DEVELOPMENT PROSPECT.
At present, the anti-therapy of root is filled with in the control of fruit root knot disease mainly chemicals, although serve certain prevention and control effect, bring again simultaneously agricultural chemicals on fruit residual, to the pollution of the environment such as soil, air, water source, kill the drawbacks such as natural enemy.The medicine that particularly instructions for use of fruit to agricultural chemicals is higher, poisonous, residual life is long prohibits the use.
Summary of the invention
The present invention is directed to above weak point, provide a kind of plant resource preparation preventing and treating fruit root knot disease and preparation method thereof, this preparation is free from environmental pollution, and with low cost, manufacturing process is simple, considerably reduces the amount of labour, safety, directly, environmental protection, efficient.
The technical solution adopted for the present invention to solve the technical problems is: a kind of plant resource preparation preventing and treating fruit root knot disease, described preparation by the composite extract of hairyvein agrimony herb, jasmine and oldenlandia diffusa herb and solvent formulated.
Described solvent is organic solvent 75% ethanol.
Prevent and treat a preparation method for the plant resource preparation of fruit root knot disease, be prepared from as follows:
Hairyvein agrimony herb, jasmine and the oldenlandia diffusa herb ratio by weight 1:1:1 is mixed, particle is broken into cracker processing, cross 60 mesh sieves, 20kg particle is put into Multifunctional Chinese medicine extraction equipment extraction still extract, add 75 liter of 75% ethanol, at the temperature of 80 ~ 84 DEG C, boiling is after 2.0 hours, more concentrated 1 hour, hairyvein agrimony herb, jasmine and oldenlandia diffusa herb composite extract condensed cream and crystalline solid; Add 75% alcohol solvent again, fully dissolve, make saturated solution, bottling sealing.
The invention has the beneficial effects as follows:
This preparation has good prevention and control effect to fruit root knot disease, not only control effect is good, and it is free from environmental pollution, with low cost, manufacturing process is simple, considerably reduce the amount of labour, safety, directly, environmental protection, efficient, and without " three wastes " discharge in production process, the raw material of production are renewable; This preparation can be used for the control of fruit root knot disease, can kill Agrobacterium tumefaciens by medication, suppresses host cell abnormal division, controls the propagation of germ, spreads, and harm, reaches the object of prevention and control root knot, for control fruit root knot disease provides technical support.
Accompanying drawing explanation
It is preparation method's concrete technology flow chart of the present invention shown in Fig. 1.
Embodiment
Describe the present invention below in conjunction with the drawings and specific embodiments:
Embodiment 1
Preparation method's concrete technology flow process of the present invention is:
(1) hairyvein agrimony herb, jasmine and oldenlandia diffusa herb are mixed by weight 1:1:1, be broken into particle with cracker processing, cross 60 mesh sieves, 20kg particle is loaded in traditional Chinese medicine extraction still and extracts;
(2) add 75% ethanol 75 liters, 80 DEG C of temperature boilings after 2.0 hours, then concentrate 1 hour, are condensed into condensed cream and crystalline solid, i.e. hairyvein agrimony herb, jasmine and oldenlandia diffusa herb composite extract;
(3) get extract and add solvent 75% ethanol again, limit edged stirs until abundant dissolving is mixed with saturated solution be the sick finished dosage form of control fruit root knot.
(4) filling respectively: stand-by by sealing in sick for control fruit root knot preparation 500ml loading plastic bottle.
The present embodiment product main component percentage by weight is:
Agrimophol 2.96%
Agrimonolide 2.59%
Methyl jasmonate 2.12%
Asperuloside 1.81%
Asperuloside acid 0.85%
75% ethanol 89.67%.
Embodiment 2
Preparation method's concrete technology flow process of the present invention is:
(1) hairyvein agrimony herb, jasmine and oldenlandia diffusa herb are mixed by weight 1:1:1, be broken into particle with cracker processing, cross 60 mesh sieves, 20kg particle is loaded in traditional Chinese medicine extraction still and extracts;
(2) add 75% ethanol 75 liters, 82 DEG C of temperature boilings after 2.0 hours, then concentrate 1 hour, are condensed into condensed cream and crystalline solid, i.e. hairyvein agrimony herb, jasmine and oldenlandia diffusa herb composite extract;
(3) get extract and add solvent 75% ethanol again, limit edged stirs until abundant dissolving is mixed with saturated solution be the sick finished dosage form of control fruit root knot.
(4) filling respectively: stand-by by sealing in sick for control fruit root knot preparation 500ml loading plastic bottle.
The present embodiment product main component percentage by weight is:
Agrimophol 3.52%
Agrimonolide 3.16%
Methyl jasmonate 2.5%
Asperuloside 1.92%
Asperuloside acid 1.21%
75% ethanol 87.69%.
Embodiment 3
Preparation method's concrete technology flow process of the present invention is:
(1) hairyvein agrimony herb, jasmine and oldenlandia diffusa herb are mixed by weight 1:1:1, be broken into particle with cracker processing, cross 60 mesh sieves, 20kg particle is loaded in traditional Chinese medicine extraction still and extracts;
(2) add 75% ethanol 75 liters, 84 DEG C of temperature boilings after 2.0 hours, then concentrate 1 hour, are condensed into condensed cream and crystalline solid, i.e. hairyvein agrimony herb, jasmine and oldenlandia diffusa herb composite extract;
(3) get extract and add solvent 75% ethanol again, limit edged stirs until abundant dissolving is mixed with saturated solution be the sick finished dosage form of control fruit root knot.
(4) filling respectively: stand-by by sealing in sick for control fruit root knot preparation 500ml loading plastic bottle.
The present embodiment product main component percentage by weight is:
Agrimophol 3.3%
Agrimonolide 2.8%
Methyl jasmonate 2.3%
Asperuloside 1.85%
Asperuloside acid 1.05%
75% ethanol 88.7%.
Embodiment 4
Preparation method's concrete technology flow process of the present invention is:
(1) hairyvein agrimony herb, jasmine and oldenlandia diffusa herb are mixed by weight 1:1:1, be broken into particle with cracker processing, cross 60 mesh sieves, 20kg particle is loaded in traditional Chinese medicine extraction still and extracts;
(2) add 75% ethanol 75 liters, 80-84 DEG C of temperature boiling after 2.0 hours, then concentrates 1 hour, is condensed into condensed cream and crystalline solid, i.e. hairyvein agrimony herb, jasmine and oldenlandia diffusa herb composite extract;
(3) get extract and add solvent 75% ethanol again, limit edged stirs until abundant dissolving is mixed with saturated solution be the sick finished dosage form of control fruit root knot.
(4) filling respectively: stand-by by sealing in sick for control fruit root knot preparation 500ml loading plastic bottle.
The present embodiment product main component percentage by weight is:
Agrimophol 2.96-3.52%
Agrimonolide 2.59-3.16%
Methyl jasmonate 2.12-2.5%
Asperuloside 1.81-1.92%
Asperuloside acid 0.85-1.21%
75% ethanol 87.69-89.67%.
The present invention prevents and treats the application technology of the plant resource preparation of fruit root knot disease, and step is as follows:
(1) in root knot generating region, each fruit trees plant before with this preparation root immersion 30 minutes of 100ug/ml, can effectively prevent root knot to occur;
(2) strike off at once when sick knurl grows to soybean grain size, this preparation scraping rear wound 120ug/ml is smeared;
(3) this preparation perfusion sterilization of the soil 150ug/ml around diseased plant, prevents and treats the generation of root knot, propagates, spreads.
The result of use of this preparation is as follows:
One, field prevention root knot generation effect test
On March 10th, 2013 at Man Zhuan town, Daiyue District, Tai'an peach growing area, peach plant before with medicament root immersion 30 minutes, peach 100 strain is soaked in each process.Chemicals treatment is sick plant resource preparation (in the embodiment products obtained therefrom) 150ug/ml of control fruit root knot, prevents and treats fruit root knot sick plant resource preparation 100ug/ml, control fruit root knot sick plant resource preparation 50ug/ml, 20% agricultural streptomycin wetting powder (production of Shijiazhuang City Wei Teng biochemical product Co., Ltd) totally 5 process such as 100ug/ml, clear water contrast, each process repeats for 3 times, randomized arrangement.Plant latter 20 days, investigation root knot incidence, each process random searching 60 strain tree counting diseased plant rate, (1 grade of sick knurl radical is 0 to disease-sensitive index; 2 grades of sick knurl roots account for total radical 1 ∕ less than 5; 3 grades of sick knurl roots account for total radical 1 ∕ 5-3 ∕ 5; 4 grades of sick knurl roots account for total radical 3 ∕ more than 5; 5 grades is the tree that dies of illness).The results are shown in Table one.
Table one: the sick plant resource preparation of control fruit root knot is to the preventive effect of Peach crown gall
Disease-sensitive index computational methods:
Disease-sensitive index=[(∑ (sick level strain number × represent numerical value))/(the representative numerical value of strain number summation × the heaviest one-level of morbidity)] × 100%
As can be seen from table one, prevention root knot generation plant resource preparation working concentration 100ug/ml, 150ug/ml all have good preventive effect to Peach crown gall, and diseased plant rate relatively reduces and is respectively: 97.0%, 97.0%; The relative reduced rate of disease-sensitive index is respectively: 96.33%, 96.67%, and all higher than contrast 20% agricultural streptomycin wetting powder 100ug/ml, diseased plant rate reduces relatively, the relative reduced rate of disease-sensitive index, the preventive effect of 89.33%, 89.67%; The preventive effect of control fruit root knot sick plant resource preparation working concentration 50ug/ml is relatively poor.Comprehensive analysis, control fruit root knot sick plant resource preparation working concentration 100ug/ml, 150ug/ml all have good preventive effect, but comparative costs is good with the working concentration bactericidal effect of 100ug/ml, less expensive.
Two, field control bretschneider pear root carninomatosis effect test
On April 16th, 2014 is at Ge Shi town, Ningyang County, Tai'an pear tree growing area, strike off at once when the sick knurl of pear tree root grows to soybean grain size, scrape rear wound with medicament to smear, soil with medicament perfusion sterilization around diseased plant, each process 100 strain, before process, (1 grade of sick knurl radical is 0 for investigation diseased plant rate and disease-sensitive index; 2 grades of sick knurl roots account for total radical 1 ∕ less than 5; 3 grades of sick knurl roots account for total radical 1 ∕ 5-3 ∕ 5; 4 grades of sick knurl roots account for total radical 3 ∕ more than 5; 5 grades is the tree that dies of illness).Wound is smeared at interval 20 days again with medicament, and the soil with medicament perfusion sterilization around diseased plant, connects and process secondary.Chemicals treatment is sick plant resource preparation (in the embodiment products obtained therefrom) 150ug/ml of control fruit root knot, prevents and treats fruit root knot sick plant resource preparation 100ug/ml, control fruit root knot sick plant resource preparation 50ug/ml, 20% agricultural streptomycin wetting powder (production of Shijiazhuang City Wei Teng biochemical product Co., Ltd) totally 5 process such as 100ug/ml, clear water contrast, each process repeats for 3 times, randomized arrangement.Secondary to spray after medicament 20 days, investigation root knot incidence, each process random searching 60 strain tree counting diseased plant rate, disease-sensitive index.The results are shown in Table two.
As can be seen from table two, control trees bubble canker plant resource preparation working concentration 100ug/ml, 150ug/ml all have good control efficiency to bretschneider pear root carninomatosis, and diseased plant rate relatively reduces and is respectively: 88.78%, 89.77%; The relative reduced rate of disease-sensitive index is respectively: 86.66%, 87.86%, and all higher than contrast 20% agricultural streptomycin wetting powder 100ug/ml, diseased plant rate reduces relatively, the relative reduced rate of disease-sensitive index, the control efficiency of 74.15%, 70.76%; The control efficiency of control fruit root knot sick plant resource preparation working concentration 50ug/ml is relatively poor.Comprehensive analysis, control fruit root knot sick plant resource preparation working concentration 100ug/ml, 150ug/ml all have good control efficiency, but comparative costs is good with the working concentration bactericidal effect of 100ug/ml, less expensive.
Table two: the sick plant resource preparation of control fruit root knot is to the control efficiency of bretschneider pear root carninomatosis
Disease-sensitive index computational methods:
Disease-sensitive index=[(∑ (sick level strain number × represent numerical value))/(the representative numerical value of strain number summation × the heaviest one-level of morbidity)] × 100%
Certainly, above-mentioned explanation is not limitation of the present invention, and the present invention is also not limited in above-mentioned citing, and the change that those skilled in the art make in essential scope of the present invention, remodeling, interpolation or replacement also should belong to protection scope of the present invention.
Claims (5)
1. prevent and treat a plant resource preparation for fruit root knot disease, it is characterized in that: described preparation by the composite extract of hairyvein agrimony herb, jasmine and oldenlandia diffusa herb and solvent formulated, main composition percentage by weight is:
Agrimophol 2.96-3.52%;
Agrimonolide 2.59-3.16%;
Methyl jasmonate 2.12-2.5%;
Asperuloside 1.81-1.92%;
Asperuloside acid 0.85-1.21%;
75% ethanol 87.69-89.67%.
2. a kind of plant resource preparation preventing and treating fruit root knot disease as claimed in claim 1, is characterized in that: described solvent is organic solvent 75% ethanol.
3. a kind of preparation method preventing and treating the plant resource preparation of fruit root knot disease as claimed in claim 1, is characterized in that: be prepared from as follows:
Hairyvein agrimony herb, jasmine and the oldenlandia diffusa herb ratio by weight 1:1:1 is mixed, particle is broken into cracker processing, cross 60 mesh sieves, 20kg particle is put into Multifunctional Chinese medicine extraction equipment extraction still extract, add 75 liter of 75% ethanol, at the temperature of 80 ~ 84 DEG C, boiling is after 2.0 hours, more concentrated 1 hour, hairyvein agrimony herb, jasmine and oldenlandia diffusa herb composite extract condensed cream and crystalline solid; Add 75% alcohol solvent again, fully dissolve, make saturated solution, bottling sealing.
4. a kind of plant resource preparation preventing and treating trees bubble canker as claimed in claim 1, is characterized in that: described Formulation is become the consumption root immersion 30 minutes of 100ug/ml-150ug/ml or smear wound or perfusion root after striking off scab.
5. hairyvein agrimony herb, jasmine and the oldenlandia diffusa herb application in the sick plant resource preparation of preparation control fruit root knot.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106007971A (en) * | 2016-05-24 | 2016-10-12 | 句容市满园春家庭农场 | Fertilizer for preventing oriental cherry agrobacterium tumefaciens and preparation method thereof |
| CN106416720A (en) * | 2016-10-13 | 2017-02-22 | 合肥润雨农业科技有限公司 | Prevention and control method for gall disease of pelargonium |
| CN107027760A (en) * | 2017-04-30 | 2017-08-11 | 陶云霞 | One Plant Extracts are used for the preparation and preparation method for preventing and treating chrysanthemum pest and disease damage |
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| CN1107472A (en) * | 1994-02-23 | 1995-08-30 | 江西省庐山植物园 | Use of cycloolefine ether terpenoid as yield increasing agent and root growth helping agent for crops |
| WO2011042978A1 (en) * | 2009-10-08 | 2011-04-14 | 小林製薬株式会社 | Dried du-zhong leaves, du-zhong leaf extract obtained from the dried du-zhong leaves and processed food comprising the du-zhong leaf extract |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106007971A (en) * | 2016-05-24 | 2016-10-12 | 句容市满园春家庭农场 | Fertilizer for preventing oriental cherry agrobacterium tumefaciens and preparation method thereof |
| CN106416720A (en) * | 2016-10-13 | 2017-02-22 | 合肥润雨农业科技有限公司 | Prevention and control method for gall disease of pelargonium |
| CN107027760A (en) * | 2017-04-30 | 2017-08-11 | 陶云霞 | One Plant Extracts are used for the preparation and preparation method for preventing and treating chrysanthemum pest and disease damage |
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Inventor after: Pang Xianwei Inventor after: Jiang Yibin Inventor after: Huang Jian Inventor after: Li Bo Inventor after: Chen Long Inventor after: Wang Yongpu Inventor after: Zhang Cangang Inventor before: Pang Xianwei Inventor before: Li Dongmei Inventor before: Huang Jian Inventor before: Li Bo Inventor before: Chen Long Inventor before: Wang Yongpu Inventor before: Zhang Cangang |
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