CN106613557A - Microbial control method for plant southern blight - Google Patents
Microbial control method for plant southern blight Download PDFInfo
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- CN106613557A CN106613557A CN201710040394.7A CN201710040394A CN106613557A CN 106613557 A CN106613557 A CN 106613557A CN 201710040394 A CN201710040394 A CN 201710040394A CN 106613557 A CN106613557 A CN 106613557A
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- plant
- lay mould
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- southern blight
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
Landscapes
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
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Abstract
The invention relates to the technical field of function microbial application and concretely provides a microbial control method for plant southern blight. In the method Penicilliumbilaii is applied to soil, roots of plants and/or whole plants, so southern blight can be effectively prevented and treated; Penicilliumbilaji VLD-11 has been preserved in China Center for Type CultureCollection of Wuhan University and the preservation code is CCTCC No.M2015690.
Description
Technical field
The invention belongs to technical field of microbe application, and in particular to a kind of microbial control method of plant southern blight.
Background technology
Southern blight（Scleritiumrolfsii）Also known as sclerotium root rot and sclerotium seedling blight, be it is a kind of the torrid zone,
The plant disease that subtropical zone generally occurs.The tomato southern blight from Florida, USA reported first in 1891（South
Droop）Since, with regard to the hot issue in the research report always research of plant disease of various plant southern blights.
Southern blight germ can cause harm various plants, but the symptom basic simlarity of diseased plant.Disease occurs mainly in seedling
Subaerial rhizome portion.When just occurring, the cortex browning in sick portion gradually develops to surrounding.White spun silk shape is produced on scab
Mycelia, radially extend more than mycelium, spread near native table on.After plant morbidity, basal part of stem and root cortex are rotten
Rotten, the conveying of moisture and nutrient is blocked, and blade flavescence is wilting, and complete stool is withered.Withered rhizome is only left wood fibre tissue,
Like " tangled skein of jute " shape, easily extract from soil.Under conditions of soil is relatively dried, sick portion is in canescence dry rot shape, sometimes visible white
The mould layer of color；When moist, sick portion is covered with white hypha body, or even peripheral ground also covers one layer of mycelium, and forms rapeseed shape
Sclerotium.
The imperfect stage of southern blight cause of disease be Deuteromycotina spore mesh (Mycelia Sterilia) Sclerotium (
SclerotiumTode ex Fr.) neat bulbil bacterium（ScleritiumrolfsiiSacc.）, the bacterium is S very strong
Soil inhabitant.The perfect stage of cause of disease belongs to the white thin,tough silk photovoltaicing leather bacteria [Corticiumrolfsii of Basidiomycotina, corticium
(Sacc.) Curzi.].Seldom occur under the sick natural conditions, only in hygrothermal environment, accidental scab edge produce load and
Basidiospore.
For soil-borne disease, crop rotation is an important prophylactico-therapeutic measures.Efficent rotation, forbids continuous cropping or susceptible posts with other
Main wheel is made, and gramineous crop crop rotation, or rice field-upland field rotation, has very much effect to preventing the disease.Research discovery, peanut and America
Ditch millet crop rotation has good preventive and therapeutic effect to southern blight.Medicinal herb growers also identify in long-term production practices with corn, Ipomoea batatas,
The shift of crops such as rape more than 3 years, can effectively prevent and treat southern blight.
In terms of chemical control, the indoor harmacological effect such as Liu Huiqing is determined and found, Tuzet, gram rotten spy, formaldehyde, Mancozeb pair
Sclerotial germination has very strong inhibitory action, and carbendazim, thiophanate methyl, Bravo also have certain effect.Medicament is further tested
As a result show, 50% carbendazim, 75% Bravo, 36% formaldehyde inhibition zone are obvious（Suppress mycelial growth）.
In terms of biological control, many reports think that Trichoderma has good result to southern blight.He Kejia Trichoderma virides
Preventing and treating seedling blight of red pepper, preventive effect is up to 69% ~ 72%；Preventing and treating konjaku southern blight, preventive effect is 65.1% ~ 69.6%.Another kind of useful life
Anti-fungal is G virens, and research finds that G virens have preventing and treating tomato, pimento and potato, the effect of pepper southern blight.It is glimmering
Light pseudomonad can suppress soybean southern blight and cotton, the generation of potato wilt.
Southern blight is a kind of general property, destructive disease, and its host range is wide, can cause crop serious during serious harm
The underproduction.Due to growing interest of the people to environmental problem and the demand to pollution-free food, biological control is one to be had before development very much
The field on way.Therefore, it is badly in need of filtering out efficient, environmentally friendly, safe biocontrol microorganisms, the morbidity and harm of southern blight is controlled, so as to take
Obtain economic and social benefit well.
The content of the invention
The present invention is solution prior art problem, there is provided a kind of microbial control method of southern blight.Methods described is
Lay mould is visitd by applying（Penicillium bilaji）Suppress the breeding of southern blight pathogenic bacteria pyrenomycetes, and then reach preventing and treating
The effect of southern blight.
One aspect of the present invention provides a kind of prevention and controls of plant southern blight, is by soil and/or in plant
Root and/or plant complete stool apply visit Lay mould bacterium solution realization.
The prevention and controls of the plant southern blight, including：
（1）Before plant seeding or plant, by 40-60mL/m2Consumption will visit Lay mould bacterium solution and uniformly be sprayed on soil, will
Top layer 10-20cm thick soil is effectively mixed；
（2）After plant setting, will visit Lay mould bacterium solution by the consumption of 100-500mL/ strains and fill and impose on plant root, 15-30 days
Fill by same amount again afterwards and apply one time, be used in conjunction 3-5 time；
（3）In southern blight early stage, by 50-100mL/m2Consumption will visit Lay mould bacterium solution be uniformly sprayed at plant complete stool and
In soil, again by same amount sprinkling one time after 5-10 days；It is used in conjunction 3-5 time.
Further, the Lay mould of visiing preferably visits Lay mould VLD-11（Penicillium bilajiVLD-11）, sieve
Selected from the soil of Laizhou City of Shandong Province Peng Jia Phosphorus Mineral Areas, Wuhan, China Wuhan University is preserved on November 23rd, 2015
China typical culture collection center, deposit number is CCTCC NO：M2015690.
The Lay mould bacterium solution miospore content of visiing is not less than 106CFU/mL。
Any one in the preferred carrot of the plant, peanut and jasmine.
The preparation method for visiing Lay mould bacterium solution, specially：First the above-mentioned Lay mould VLD-11 that visits is inoculated into PDA solids
On culture medium, 30 DEG C are cultivated 3-4 days to spore maturation, and the spore grown is washed down with appropriate SPSS, are transferred into horse
In bell potato fluid nutrient medium, it is sufficiently mixed uniformly, cultivates 4-6 days in 30 DEG C, obtains final product and visit Lay mould bacterium solution.
The method that the present invention is provided is by applying the preventing and treating for visiing the realization of Lay mould bacterium solution to plant southern blight.It is described to visit Lay
Mould VLD-11 respectively reaches 85.9% and 82.6% to the inhibiting rate of delphinium grandiflorum pyrenomycetes and Sclerotium rolfsii, and fungistatic effect is notable.
The Lay mould VLD-11 that visits can effectively prevent and treat plant southern blight.In the preventing and treating to carrot southern blight, by complete stool and soil
Earth visits 100 times of dilutions of Lay mould described in spraying, and the incidence of disease of carrot southern blight can be reduced into less than 0.5%, prevents and treats efficiency
More than 88%；In the preventing and treating to peanut sclerotium rolfsii, soil is processed first with the Lay mould of visiing, in southern blight morbidity
Initial stage, then complete stool and soil are rationally sprayed, the disease index of peanut sclerotium rolfsii can be effectively reduced, 50 times of dilutions are to flower
That gives birth to southern blight averagely prevents and treats efficiency high up to 83.6%；In the preventing and treating to jasmine southern blight, by direct in jasmine root of flower seedling
Filling visits Lay mould VLD-11 described in applying, and can effectively reduce the incidence of disease of later stage jasmine southern blight, and 100 times of dilutions are to southern blight
Prevent and treat efficiency higher than 85%, effect is significant.
Specific embodiment
That what is selected in the embodiment of the present invention visits Lay mould VLD-11（Penicillium bilajiVLD-11）, screening is certainly
The soil of Laizhou City of Shandong Province Peng Jia Phosphorus Mineral Areas, is preserved in the China of Wuhan, China Wuhan University on November 23rd, 2015
Type Tissue Collection, deposit number is CCTCC NO：M2015690.
The reagent that the present invention is applied can be using arbitrary money commercially available prod, with reference to specific embodiment to the present invention's
Method is described in detail.
Embodiment 1 visits Lay mould bacteriostatic experiment
1st, the preparation of Lay mould VLD-11 bacterium solutions is visitd：
First the above-mentioned Lay mould VLD-11 that visits is inoculated on PDA solid mediums, 30 DEG C are cultivated 3-4 days to spore maturation, by length
Good spore is washed down with appropriate SPSS, is transferred in potato fluid nutrient medium, is sufficiently mixed uniformly, in 30 DEG C of trainings
Support 4 days, zymotic fluid miospore content is about 109CFU/mL。
2nd, visit Lay mould VLD-11 to the inhibitory action 1 of pyrenomycetes, Lay mould VLD-11 will be visitd be seeded to PDA culture medium
Flat board, 30 DEG C of culture 7d；
2nd, the PDA culture medium flat board of step 1 is taken into, card punch beats the bacteria cake (biocontrol microorganisms for taking a diameter of 5mm in bacterium colony forward position
Cake)；3rd, respectively delphinium grandiflorum pyrenomycetes and Sclerotium rolfsii single bacterium colony are seeded into PDA culture medium flat board, 30 DEG C of culture 7d；4th, take
The PDA culture medium flat board of step 3 is completed, card punch beats the bacteria cake (pathogenic bacteria cake) for taking a diameter of 5mm in bacterium colony forward position； 5、
New PDA culture medium flat board is taken, the culture medium for taking a diameter of 5mm is beaten with card punch, as tester.6th, packet transaction（1）
Test group：New PDA culture medium flat board is taken, being placed above 1 biological and ecological methods to prevent plant disease, pests, and erosion bacteria cake and 1 pathogenic bacteria cake, two bacteria cake centers
Air line distance is 3cm, and 30 DEG C are cultivated；（2）Control group：New PDA culture medium flat board is taken, 1 tester and 1 is being placed above
Individual pathogenic bacteria cake, the air line distance at both centers is 3cm, and 30 DEG C are cultivated.7th, the bacterium colony of pathogenic bacteria is measured after cultivating 3 days respectively
Diameter, calculates inhibiting rate.
Inhibiting rate=(control group pathogenic bacteria colony diameter-test group pathogenic bacteria colony diameter) ÷ control group pathogenic bacteria bacterium colonies
Diameter × 100%.
As a result show：Visit Lay mould VLD-11 and 85.9% is respectively reached to the inhibiting rate of delphinium grandiflorum pyrenomycetes and Sclerotium rolfsii
With 82.6% (mean value of three repetitions), illustrate it is described visit Lay mould VLD-11 the growth and breeding of pyrenomycetes is had it is stronger
Inhibitory action, is expected to be applied to the preventing and treating of southern blight.
Embodiment 2 visits prevention effect of the Lay mould to carrot southern blight
1st, place is tested：Qingdao Pingdu south villages and small towns carrot region of disease
2nd, experimental technique
Three treatment groups are chosen, each ridge carrot for the treatment of group 3, per about 100, ridge carrot, sets up protection row between treatment group.
Above-mentioned Lay mould VLD-11 zymotic fluids of visiing are diluted with water into respectively 100 times, 200 times, 400 times.In carrot early stage, by 50-
100mL/m2Consumption will visit Lay mould bacterium solution with electrostatic atomiser complete stool and soil will fully be sprayed, interval is pressed again for 5 days
Same amount is sprayed 1 time, is prevented and treated 3 times altogether.Control group is the clear water for spraying same amount.After last time preventing and treating 6 days, check recklessly
The treatment of radish southern blight.
3rd, interpretation of result
Prevent and treat the computing formula=［ 1- of efficiency（The diseased plant rate of the diseased plant rate/control of process）］ × 100%
As a result it is as shown in table 1：
Table 1 visits prevention effects of the Lay mould VLD-11 to carrot southern blight
Can be seen that in carrot southern blight early stage from the data of table 1, by spraying to complete stool and soil Lay mould is visitd
VLD-11, can significantly inhibit the incidence of disease of southern blight, and 100 times of zymotic fluid dilutions can reduce the incidence of disease of carrot southern blight
To less than 0.5%, efficiency is prevented and treated more than 88%, effect is significant.
Embodiment 3 visits prevention effects of the Lay mould VLD-11 to peanut sclerotium rolfsii
1st, place is tested：Qingdao Jiaonan City peanut cultivation region of disease
2nd, experimental technique：
To visit Lay mould VLD-11 zymotic fluids add water respectively dilute 50 times, 100 times, 200 times, in the peanut seeding phase, first by 40-
60mL/m2Consumption will visit Lay mould bacterium solution and uniformly be sprayed on soil, 10-20cm thick soil in top layer is effectively mixed
It is even, then sowed again；In peanut sclerotium rolfsii early stage, by 50-100mL/m2Consumption electrostatic atomiser will visit Lay
Mould bacterium solution is fully sprayed to complete stool and soil, and interval is sprayed 1 time again for 5 days by same amount, and 3 times are sprayed altogether.Last
After secondary preventing and treating 6 days, the treatment of peanut sclerotium rolfsii is checked.Control group uses clear water.
3rd, interpretation of result：
Disease index=［ ∑（The sick level value × level morbidity strain tree）/ (morbidity total strain number × highest disease level value) ］ × 100%
Prevent and treat efficiency=［ 1-（The disease index of the disease index/control of process）］ × 100%
As a result it is as shown in table 2：
Table 2 visits prevention effects of the Lay mould VLD-11 to peanut sclerotium rolfsii
Can be seen that in peanut seeding from the data of table 2, the Lay mould VLD-11 that visits provided first with the present invention enters to soil
Row is processed, and in southern blight early stage, then complete stool and soil is rationally sprayed, and can effectively reduce the state of an illness of peanut sclerotium rolfsii
Index.Visit and efficiency high is averagely prevented and treated up to 83.6% to peanut sclerotium rolfsii when Lay mould VLD-11 zymotic fluids dilute 50 times, effect shows
Write.
Embodiment 4 visits prevention effects of the Lay mould VLD-11 to jasmine southern blight
1st, place is tested：
The withered peach flowers market jasmine garden of Qingdao City's Laoshan District.
2nd, Setup Experiments
If three treatment groups, will visit Lay mould VLD-11 zymotic fluids add water respectively dilute 100 times, 200 times, 400 times.
At the beginning of 4 months 2016, Jasmine after seedling is being transplanted, Lay mould VLD-11 will visitd respectively by the consumption of 300 mL/ strains dilute
The root that liquid is applied directly to flower seedling is released, is applied again after 20 days 1 time, 4 times are applied altogether.Per 3 repetitions for the treatment of group, each repeats 50 plants
Jasmine.Control group applies clear water.
3rd, jasmine southern blight prevention effect statistics
At the beginning of 11 months 2016, each treatment group jasmine incidence is investigated, calculate the prevention effect of jasmine southern blight.
Severity Scaling standard：0 grade-have no yellows leaf disease branch；1 grade-withered and yellow branches and leaves ratio of catching an illness accounts for less than 25%；3 grades-
Catch an illness withered and yellow branches and leaves ratio >=25%, but≤50%；5 grades-withered and yellow branches and leaves ratio of catching an illness>50%, but plant is not withered；7 grades-whole strain
It is withered.
Disease index=［ ∑（The sick level value × level morbidity strain tree）/ (morbidity total strain number × highest disease level value) ］ × 100%；
Prevent and treat efficiency=［ 1-（The disease index of the disease index/control of process）］ × 100%；
As a result it is as shown in table 3：
Table 3 visits prevention effects of the Lay mould VLD-11 to jasmine southern blight
From the results shown in Table 3, by jasmine root of flower seedling directly fill present invention offer is provided visit Lay mould VLD-11,
The incidence of disease of later stage jasmine southern blight can be effectively reduced, 100 times of dilutions of Lay mould VLD-11 is visitd and efficiency is prevented and treated to southern blight
Higher than 85%, effect is significant.
Except carrot, outside peanut and jasmine, the present invention provide visit Lay mould VLD-11 can also to capsicum, shallot,
The southern blight of the plants such as walnut, masson pine, apple, oranges and tangerines has significant prevention effect, prevents and treats efficiency and reaches more than 80%, should
It is extensive with prospect.
Claims (6)
1. a kind of prevention and controls of plant southern blight, it is characterised in that described method is by soil and/or in plant
Root and/or plant complete stool apply visit Lay mould bacterium solution realization.
2. prevention and controls as claimed in claim 1, it is characterised in that described method includes：
（1）Before plant seeding or plant, by 40-60mL/m2Consumption will visit Lay mould bacterium solution and uniformly be sprayed on soil, will
Top layer 10-20cm thick soil is effectively mixed；
（2）The filling of Lay mould bacterium solution will be visitd by the consumption of 100-500mL/ strains and impose on plant root, press same amount after 15-30 days again
Filling is applied one time, is used in conjunction 3-5 time；
（3）By 50-100mL/m2Consumption will visit Lay mould bacterium solution and uniformly be sprayed in plant complete stool and soil, after 5-10 days again
Spray one time by same amount；It is used in conjunction 3-5 time.
3. prevention and controls as claimed in claim 1 or 2, it is characterised in that the described deposit number for visiing Lay mould is CCTCC
NO：M2015690.
4. prevention and controls as claimed in claim 1 or 2, it is characterised in that described visits Lay mould bacterium solution miospore content not
Less than 106CFU/mL。
5. prevention and controls as claimed in claim 1 or 2, it is characterised in that described plant is in carrot, peanut and jasmine
Any one.
6. prevention and controls as claimed in claim 1 or 2, it is characterised in that the described preparation method for visiing Lay mould bacterium solution, tool
Body is：Lay mould will first be visitd to be inoculated on PDA solid mediums, 30 DEG C are cultivated 3-4 days to spore maturation, and the spore grown is used
Appropriate SPSS is washed down, is transferred in potato fluid nutrient medium, is sufficiently mixed uniform, cultivates 4-6 days in 30 DEG C, i.e.,
Get Bai Lai mould bacterium solutions.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107771860A (en) * | 2017-10-24 | 2018-03-09 | 慕恩（北京）生物科技有限公司 | A kind of microorganism composite seed coating agent and its production method |
| CN113832049A (en) * | 2021-08-13 | 2021-12-24 | 湖北省农业科学院中药材研究所 | Coptis southern blight biocontrol bacterium pseudomonas fluorescens HT1 and application thereof |
| CN114424725A (en) * | 2021-11-29 | 2022-05-03 | 湖南省蔬菜研究所 | Method for preventing and treating southern blight of cultivated peppers by using trichloroisocyanuric acid |
| CN114831126A (en) * | 2022-04-27 | 2022-08-02 | 湖南神隆海洋生物工程有限公司 | Method for preventing and treating crop soil-borne diseases by using trichloroisocyanuric acid and application |
| CN116162564A (en) * | 2022-08-18 | 2023-05-26 | 西南科技大学 | Achromobacter JY-2-3R strain for biocontrol of aconitum carmichaeli and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107771860A (en) * | 2017-10-24 | 2018-03-09 | 慕恩（北京）生物科技有限公司 | A kind of microorganism composite seed coating agent and its production method |
| CN113832049A (en) * | 2021-08-13 | 2021-12-24 | 湖北省农业科学院中药材研究所 | Coptis southern blight biocontrol bacterium pseudomonas fluorescens HT1 and application thereof |
| CN114424725A (en) * | 2021-11-29 | 2022-05-03 | 湖南省蔬菜研究所 | Method for preventing and treating southern blight of cultivated peppers by using trichloroisocyanuric acid |
| CN114424725B (en) * | 2021-11-29 | 2023-11-24 | 湖南省蔬菜研究所 | Method for preventing and treating cultivated capsicum southern blight by using trichloroisocyanuric acid |
| CN114831126A (en) * | 2022-04-27 | 2022-08-02 | 湖南神隆海洋生物工程有限公司 | Method for preventing and treating crop soil-borne diseases by using trichloroisocyanuric acid and application |
| CN116162564A (en) * | 2022-08-18 | 2023-05-26 | 西南科技大学 | Achromobacter JY-2-3R strain for biocontrol of aconitum carmichaeli and application thereof |
| CN116162564B (en) * | 2022-08-18 | 2023-12-01 | 西南科技大学 | Achromobacter JY-2-3R strain for biocontrol of aconitum carmichaeli and application thereof |
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