CN103382451A - Streptomyces griseochromogenes YN-6 and application thereof to club root control - Google Patents
Streptomyces griseochromogenes YN-6 and application thereof to club root control Download PDFInfo
- Publication number
- CN103382451A CN103382451A CN2013103417464A CN201310341746A CN103382451A CN 103382451 A CN103382451 A CN 103382451A CN 2013103417464 A CN2013103417464 A CN 2013103417464A CN 201310341746 A CN201310341746 A CN 201310341746A CN 103382451 A CN103382451 A CN 103382451A
- Authority
- CN
- China
- Prior art keywords
- grey
- crop
- club root
- root
- produced
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention provides a Streptomyces griseochromogenes YN-6 separated from germ-carrying soil of cruciferae crops infected with club root, and the CCTCC No is M2013187. The strain YN-6 has good control effect on the club root of the cruciferae crops through verification of club root germ resting spore germination inhabitation and greenhouse pot experiments. The strain is high in adaptability and colonization capacity under field natural conditions, not prone to antagonistic reaction with complicated microorganism species in field soil, the disease control effect is stable, defects of chemical control, disease-resistant variety and agricultural control methods are effectively overcome, and the strain is an ideal anti-biological inoculant applicable to field club root control.
Description
Technical field
The present invention relates to the biological control field, specifically, relate to a strain grey and produce look streptomycete YN-6 and the application in preventing and treating club root thereof.
Background technology
The crop in cruciferae club root is to infect by rape plasmodiophora brassicae (Plasmodiophora brassicae) the worldwide soil that causes to pass Plant diseases.The rape plasmodiophora brassicae is obligate parasite, survives the winter in the soil of invalid body and the barnyard manure that do not become thoroughly decomposed with its statospore, and survival ability is extremely strong, generally can survive at least 8 years, and condition is suitable survived more than 15 years.Main prophylactico-therapeutic measures for the crop in cruciferae club root at present has chemical prevention, disease-resistant variety utilization, cultural control and biological control etc.
Chemical prevention is the more general method of preventing and treating that adopts, derosal, quintozene, m-tetrachlorophthalodinitrile, good fortune are handsome that (fluazinam), section's good (cyanogen frost) etc. all have certain prevention effect to club root, effectively preventing disease in a short time, the output of guaranteeing crop can not be greatly affected, but the cost of this kind method control is relatively high, and most medicament can leave residually on crop, and the edible safety of crop is had a huge impact.
Breeding resistant variety is the Perfected process of preventing and treating club root.But because disease-resistant variety cultivate to need of long durationly, the cycle is long, and there is Physiological Race Differentiation in pathogen plasmodiophora, and disease-resistant variety with regard to susceptibleization, therefore, be controlled club root by disease-resistant variety also very difficult after the application several years fully.
The cultural control method is as hoting water treatment of seeds, enrich white lime or plant ash etc., and these treatment processs also have certain limitation, as executes white lime and changed soil ph, but life-time service can destroy Soil structure, is not suitable for life-time service.
Biological control namely utilizes beneficial microorganism to kill or suppresses the growth of pathogenic micro-organism and reduce the quantity of pathogenic micro-organism, and Plant diseases occurs, a class measure of development thereby control.Consider from the sustainability of controlling Plant diseases, biological control is a class green safety effectively preventing method.
Summary of the invention
The purpose of this invention is to provide a strain grey and produce look streptomycete YN-6 and the application in control crop in cruciferae club root thereof.
In order to realize the object of the invention, the invention provides a strain grey and produce look streptomycete (Streptomyces griseochromogenes) YN-6, this bacterium be from dye crop in cruciferae that club root is arranged with the isolated streptomycete that the crop in cruciferae club root is had better prevention effect soil bacteria, now be preserved in Chinese Typical Representative culture collection center, address: Wuhan, China, Wuhan University, postcode 430072, deposit number CCTCC No:M2013187, preservation date on May 8th, 2013.
Above-mentioned grey is produced the cultural method of look streptomycete YN-6: grey is produced look streptomycete YN-6 be inoculated in the pH value in the Gause I substratum of 7.2-7.4, at 24-28 ℃, cultivate 5-6d under dark condition.
Adopt plate inserted sheet method to observe the form that grey is produced look streptomycete YN-6: the elongated multi-branched of substrate mycelium, straight shape or ripple are bent, do not rupture; The aerial hyphae spiral, relatively sturdy, easy fracture; Spore is partially cylindrical, and there is depression (Fig. 1 and 2) on the surface.
The physio-biochemical characteristics of bacterial strain YN-6: produce melanochrome and H
2S can utilize D-Fructose, glucose, sucrose, wood sugar, PEARLITOL 25C, can not make gelatine liquefication, can not make Starch Hydrolysis (not producing amylase), can not decomposition of cellulose (not producing cellulase), can not utilize L-rhamnosyl, L-arabinose, inositol.
Grey is produced look streptomycete YN-616S rDNA sequencing result as shown in Seq ID No.1.
The present invention also provides the biocontrol fungicide that is produced look streptomycete YN-6 preparation by grey.
The present invention also provides grey to produce the application in control crop in cruciferae (as Chinese cabbage, four seasons cream Plantula Brassicae chinensis, stone row cabbage heart etc.) club root of look streptomycete YN-6 or described biocontrol fungicide.
Streptomycete YN-6 is after activation on Gause I liquid nutrient medium flat board, 2 bacterium cake accesses of beating cut-off footpath 5mm with punch tool are equipped with in the 250mL triangular flask of 150mL Gause I liquid nutrient medium, under 28 ℃ of 180r/min conditions, shaking culture 6d in shaking table, filter with sterile gauze (4 layers), obtain streptomycete YN-6 nutrient solution.
Carry out the greenhouse pot culture test: adopt bacterium soil inoculation method to infect club root by crop, respectively at seeding time, after planting 7d, after planting 14d employing root-pouring method impose on streptomycete YN-6 nutrient solution or described biocontrol fungicide the root of crop, the preferred 20mL of each every alms bowl 15-25mL() 20mL, the preferred 30mL of every 3-5d cast Hoagland nutritive medium 25-35mL().
The present invention produces look streptomycete YN-6 from what dye crop in cruciferae that club root is arranged with the grey that is separated to soil bacteria, by pathogen plasmodiophora statospore Germination suppression and greenhouse pot culture verification experimental verification bacterial strain YN-6 are had prevention effect preferably to the crop in cruciferae club root.This bacterial strain under the natural condition of field adaptive faculty and colonazition strong, be difficult for field soil in antagonistic action occurs between the microbe species of complexity, prevent and treat the disease effect stability, effectively having evaded the deficiency that exists in the methods such as chemical prevention, disease-resistant variety and cultural control, is the desirable biocontrol fungicide that is suitable for land for growing field crops club root control.
Description of drawings
Fig. 1 is that in the embodiment of the present invention 2, grey is produced the form (* 600) of look streptomycete YN-6 on the Gause I substratum.
Fig. 2 is the spore chain form (* 30000) that in the embodiment of the present invention 2, grey is produced look streptomycete YN-6.
Fig. 3 is that in the embodiment of the present invention 2, grey is produced the cultivation results of look streptomycete YN-6 on the different culture media flat board.
Fig. 4 adopts dull and stereotyped face-off culture method to identify that streptomycete YN-6 is to the inhibition of various pathogenic bacteria in the embodiment of the present invention 2.
Embodiment
Following examples are used for explanation the present invention, but are not used for limiting the scope of the invention.If do not specialize, the conventional means that in embodiment, technique means used is well known to those skilled in the art, the raw materials used commercial goods that is.
Embodiment 1 grey is produced the preparation of look streptomycete YN-6 nutrient solution
To produce look streptomycete YN-6(CCTCC No:M2013187 with the grey that is separated to soil bacteria from what dye crop in cruciferae that club root is arranged) after the activation of Gause I liquid nutrient medium flat board, 2 bacterium cake accesses of beating cut-off footpath 5mm with punch tool are equipped with in the 250mL triangular flask of 150mL Gause I liquid nutrient medium, under 28 ℃ of 180r/min dark conditions, shaking culture 6d in shaking table, filter with sterile gauze (4 layers), obtain streptomycete YN-6 nutrient solution.
Embodiment 2 is by to the experiment of pathogen plasmodiophora statospore Germination suppression and the greenhouse pot culture test evaluation bacterial strain YN-6 prevention effect to the crop in cruciferae pathogen plasmodiophora
The indicator strain that uses in the present embodiment is orange anthrax bacteria (Colletotrichum geloeosporiolides), ustilaginoidea virens (Ustiloginoidea virens), Phytophthora capsici germ (Phytophthorac apsici), capsicum point spore anthrax bacteria (Colletotrichum spp.), Pyricularia oryzae (Pyricularia oryzae).
1 pair of pathogen plasmodiophora statospore Germination suppression experiment
Bacterial strain YN-6 nutrient solution, pathogen plasmodiophora statospore suspension, Collina juice are added in the triangular flask of sterilization with the amount of 2mL, 2mL, 20mL respectively, replace the Antagonistic Fungi nutrient solution as contrast take sterilized water, pH value 6.3,24 ℃ of dark culturing, sprout situation with lichens red colouring 3min microscopy statospore after 5d, repeat 3 times, it is 61.75% to pathogen plasmodiophora statospore Germination suppression rate that result shows.
2 greenhouse pot culture tests
Adopt bacterium soil inoculation method to infect club root by crop, respectively at seeding time, after planting 7d, after planting 14d adopts root-pouring method streptomycete YN-6 nutrient solution to be imposed on the root of crop, each every alms bowl waters 20mL.Clear water, 75% m-tetrachlorophthalodinitrile (* 600 times of liquid) are set are contrast, totally 6 processing, each processes 6 alms bowls, every alms bowl 5 strains.Every 3-5d cast Hoagland nutritive medium is 30mL approximately, plantation approximately 30d " Invest, Then Investigate " club root a situation arises (table 1 and table 2).
The prevention effect of table 1YN-6 to susceptible kind four seasons cream Plantula Brassicae chinensis club root
The prevention effect of table 2.YN-6 to the special pure breeding stone row of susceptible kind cabbage heart club root
Cabbage clubroot is to infect by Protozoa rape plasmodiophora brassicae (P.brassicae) the worldwide soil that causes to pass Plant diseases, the main harm root, form tumour not of uniform size, cause when serious that plant butt rot and even complete stool are withered, cause heavy economic losses.The major measure that present control crop in cruciferae club root is taked has the chemical prevention of comprising, disease-resistant variety utilization, and because soil-borne disease chemical control consumption is large, there is hidden danger in cultural control etc. to the brassicaceous vegetable edible safety; Disease-resistant variety is few at present, can not satisfy need of production; Cultural control only can be as the assisting in preventing and treating measure.It is a kind of safe, effective, free of contamination method of preventing and treating that the application antagonistic microbe carries out this disease of biological control.Consider from the angle of Sustainable development, screening biocontrol microorganisms controlling plant diseases is considered to safe and effective, the free of contamination method of preventing and treating.
The present invention is separated to bacterial strain YN-6 from the band soil bacteria that dyes the crop in cruciferae that club root is arranged, by the flat board culture experiment that stands facing each other, find this bacterial strain to multiple cause of disease indicator, as orange anthrax bacteria (Colletotrichum geloeosporiolides), ustilaginoidea virens (Ustiloginoidea virens), Phytophthora capsici germ (Phytophthora capsici), capsicum point spore anthrax bacteria (Colletotrichum spp.), Pyricularia oryzae (Pyricularia oryzae) etc., antagonistic effect is preferably arranged all.
Fig. 3 is for using the dull and stereotyped result of cultivating grey product look streptomycete YN-6 of different culture media.
Fig. 4 is for adopting dull and stereotyped face-off culture method to identify that streptomycete YN-6 is to the inhibition of various pathogenic bacteria.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements, all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (8)
1. grey is produced look streptomycete (Streptomyces griseochromogenes) YN-6, and its preserving number is CCTCC No:M2013187.
2. the described grey of claim 1 is produced the cultural method of look streptomycete YN-6, it is characterized in that, grey is produced look streptomycete YN-6 be inoculated in the pH value in the Gause I substratum of 7.2-7.4, at 24-28 ℃, cultivates 5-6d under dark condition.
3. produced the biocontrol fungicide of look streptomycete YN-6 preparation by the described grey of claim 1.
4. the described grey of claim 1 is produced look streptomycete YN-6 or the application of the described biocontrol fungicide of claim 3 in control crop club root.
5. application according to claim 4, is characterized in that, described crop is crop in cruciferae.
6. application according to claim 5, is characterized in that, described crop in cruciferae is Chinese cabbage, stone row cabbage heart.
7. according to claim 4-6 described application of any one, it is characterized in that, adopt root-pouring method that grey is produced the root that look streptomycete YN-6 nutrient solution or described biocontrol fungicide impose on the crop that catches an illness, each every alms bowl 15-25mL, every 3-5d cast Hoagland nutritive medium 25-35mL.
8. application according to claim 7, is characterized in that, adopts root-pouring method that grey is produced the root that look streptomycete YN-6 nutrient solution or described biocontrol fungicide impose on the crop that catches an illness, each every alms bowl 20mL, every 3-5d cast Hoagland nutritive medium 30mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310341746.4A CN103382451B (en) | 2013-08-07 | 2013-08-07 | Streptomyces griseochromogenes YN-6 and application thereof to club root control |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310341746.4A CN103382451B (en) | 2013-08-07 | 2013-08-07 | Streptomyces griseochromogenes YN-6 and application thereof to club root control |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103382451A true CN103382451A (en) | 2013-11-06 |
| CN103382451B CN103382451B (en) | 2014-12-10 |
Family
ID=49490381
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310341746.4A Active CN103382451B (en) | 2013-08-07 | 2013-08-07 | Streptomyces griseochromogenes YN-6 and application thereof to club root control |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103382451B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439591A (en) * | 2018-12-06 | 2019-03-08 | 四川农业大学 | A kind of dead color streptomyces chromogenes SICAU417 and its application |
| CN116240115A (en) * | 2023-03-12 | 2023-06-09 | 西南大学 | Streptomyces griseus capable of preventing and treating pepper epidemic disease and application thereof |
| CN116240115B (en) * | 2023-03-12 | 2024-05-03 | 西南大学 | Streptomyces griseus capable of preventing and treating pepper epidemic disease and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101311262A (en) * | 2007-05-22 | 2008-11-26 | 上海市农药研究所 | Streptomyces griseus and uses thereof |
| CN101416641A (en) * | 2008-09-12 | 2009-04-29 | 云南农业大学 | Biological preparation capable of preventing and treating cruciferae club root and use thereof |
-
2013
- 2013-08-07 CN CN201310341746.4A patent/CN103382451B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101311262A (en) * | 2007-05-22 | 2008-11-26 | 上海市农药研究所 | Streptomyces griseus and uses thereof |
| CN101416641A (en) * | 2008-09-12 | 2009-04-29 | 云南农业大学 | Biological preparation capable of preventing and treating cruciferae club root and use thereof |
Non-Patent Citations (3)
| Title |
|---|
| L.H. CHEAH 等: "BIOLOGICAL CONTROL OF CLUBROOT ON CAULIFLOWER WITH TRICHODERMA AND STREPTOMYCES SPP.", 《NEW ZEALAND PLANT PROTECTION》, vol. 53, 31 December 2000 (2000-12-31), pages 18 - 21 * |
| LEE S O 等: "Isolation and characterization of endophytic actinomycetes from Chinese cabbage roots as antagonists to Plasmodiophora brassicae", 《J MICROBIOL BIOTECHNOL》, vol. 18, no. 11, 31 December 2008 (2008-12-31), pages 1741 - 1746 * |
| 王靖 等: "十字花科根肿病研究进展", 《植物保护》, vol. 37, no. 6, 31 December 2011 (2011-12-31), pages 153 - 158 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439591A (en) * | 2018-12-06 | 2019-03-08 | 四川农业大学 | A kind of dead color streptomyces chromogenes SICAU417 and its application |
| CN116240115A (en) * | 2023-03-12 | 2023-06-09 | 西南大学 | Streptomyces griseus capable of preventing and treating pepper epidemic disease and application thereof |
| CN116240115B (en) * | 2023-03-12 | 2024-05-03 | 西南大学 | Streptomyces griseus capable of preventing and treating pepper epidemic disease and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103382451B (en) | 2014-12-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107446847B (en) | Bacillus belgii GT11 and application thereof | |
| CN101473853B (en) | Application of Bacillus cereus Bacillus cereusCMCC63305 in agriculture field | |
| CN103451135B (en) | Bacillus subtilis M3 and application thereof | |
| CN106497831B (en) | A kind of prevention and treatment Phytophthora nicotianae disease composite bacteria agent and its preparation method and application | |
| CN105368720B (en) | Cotton endogenetic fungus CEF-082 and its application in cotton verticillium wilt prevention and treatment | |
| CN102021122B (en) | High-efficiency insecticidal fungus and applications thereof | |
| CN102899266B (en) | Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 and application | |
| CN104130958A (en) | Bacillus subtilis and application of bacillus subtilis in preventing and curing peony root-knot nematode | |
| CN104974965A (en) | Bacillus subtilis JN005 and application thereof in prevention and control of rice blast | |
| CN103087960A (en) | Bacillus amyloliquefaciens FQS38 and application thereof | |
| CN106701623B (en) | The atrophy bacillus of one plant of antagonism fructus lycii root rot and its application | |
| CN106754426A (en) | A kind of trichoderma asperellum and its application | |
| CN106212281B (en) | A kind of method for tissue culture for improving banana survival rate | |
| CN104611254A (en) | BaciIllus polymyxa KN-03 and culturing method and use thereof | |
| CN106942276A (en) | Bacillus subtilis BAB 1 prevents and treats the application of cucurbits powdery mildew | |
| CN104738093A (en) | Preparation method for bacillus coagulans bacterial suspension | |
| CN108048357A (en) | A kind of bacillus subtilis and its cultural method | |
| CN105112304B (en) | A kind of porous smoke pipe bacterium Gao Shi 15 and its preparation for preventing and treating vegetables root disease | |
| CN107460151A (en) | A kind of spherical lysine bacillus and its application for preventing and treating Meloidogyne incognita | |
| CN112889843B (en) | Killed rice-germ bacillus wettable powder for preventing and treating citrus red spiders and application thereof | |
| CN103451112A (en) | Saline-alkali tolerant trichoderma longibrachiatum and application thereof | |
| CN103451111A (en) | Saline-alkali tolerant trichoderma harzianum and application thereof | |
| CN106754529B (en) | The bacillus amyloliquefaciens of one plant of antagonism fructus lycii root rot and its application | |
| CN103146600A (en) | Antagonistic bacteria for prevention and treatment of tobacco bacterial wilt and application thereof | |
| CN102732430A (en) | Aspergillus niger strain and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |