CN105594752A - Method for preventing and controlling rice Hirschmanniella oryzae diseases by using plant material ligusticum chuanxiong hort - Google Patents
Method for preventing and controlling rice Hirschmanniella oryzae diseases by using plant material ligusticum chuanxiong hort Download PDFInfo
- Publication number
- CN105594752A CN105594752A CN201410639378.6A CN201410639378A CN105594752A CN 105594752 A CN105594752 A CN 105594752A CN 201410639378 A CN201410639378 A CN 201410639378A CN 105594752 A CN105594752 A CN 105594752A
- Authority
- CN
- China
- Prior art keywords
- rice
- extract
- ligusticum chuanxiong
- rhizoma chuanxiong
- chuanxiong hort
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a method for preventing and controlling rice Hirschmanniella oryzae diseases by using a plant material ligusticum chuanxiong hort, belongs to the technical field of biological prevention and control of rice nematode diseases, and particularly relates to a method for reducing harm of Hirschmanniella oryzae on rice by using a ligusticum chuanxiong hort extract. The method comprises: crushing ligusticum chuanxiong hort, screening, carrying out extraction concentration on the screened ligusticum chuanxiong hort by adopting water or an organic solvent as an entrainer to obtain an extract, diluting the ligusticum chuanxiong hort extract or combining the ligusticum chuanxiong hort and a pesticide fertilizer, and directly spraying on the rice or dipping the root during the seedling transplanting. According to the present invention, the ligusticum chuanxiong hort extract can effectively inhibit and kill Hirschmanniella oryzae, can reduce the harm of Hirschmanniella oryzae on rice, achieves the yield increase purpose, provides safety for beneficial organisms, and does not produce environmental pollution.
Description
Technical field
The invention belongs to paddy rice nematodiasis biological prevention field, be specifically related to one utilize Ligusticum wallichii (LigusticumchuanxiongHort) method of the latent root nematode of extract prevention and control to rice hazard, more specifically, the present invention relates to the use of Rhizoma Chuanxiong extract on paddy rice.
Background technology
Hirschmanniella Oryzae has become global paddy rice harmful organism, and it infects the disease that paddy rice causes and causes Severe Reduction and be difficult to control. Adopt the method for rice field-upland field rotation can effectively prevention and control disease to occur, but helpless can rotation crop varieties single, and because rice is the daily staple food grains of people, long-term rice field-upland field rotation minimizing Monitoring of Paddy Rice Plant Area thing relates to grain security and social stability and is difficult to continue to carry out; Adopt chemical agent can retrieve partial loss in conjunction with agronomic measures control; But the concern to healthy and environment along with social progress and the people, limitation and drawback that chemical agent uses day by day manifest; More and more pay attention to today of environmental problem and pollution-free food production people, environmental pollution is serious, disabled in many countries and regions successively to the harmful height poison of human and livestock health, high nematocide residual and " three cause ", as being once widely used in medicament-Furadan, Nemacur, the ethoprophos of rice field control nematode. Biogenic prevention and control Chemicals is more aobvious important. But the preparation that the microorganisms such as using fungus, bacterium, food line nematode and product thereof, plant source active component control Rice-root Nematode Disease In Hunan Province are applied in production there is no open report, still relies on chemical agent on control in field.
More than one, since century, agricultural science and technology worker has made a large amount of test and studies to the control of Rice-root Nematode Disease In Hunan Province, can be summarized as following several respects:Agricultural measures rice field-upland field rotation is effective measures to the control of the root nematode disease of diving. But because latent root nematode host range is very extensive, the object of crop rotation is very limited. Disease tolerant variety: in to paddy rice and the interrelationship study of latent root nematode, there is not yet the report of a certain rice varieties to the root nematode high resistance of diving.The Agro-chemicals control optimum period of Agro-chemicals control Hirschmanniella Oryzae be period of seedling establishment to beginning the tiller phase, be secondly transplanting time; Use ethoprophos 5%SG75kghm-2Dispenser in tillering stage, can make increasing production of rice 4.2%-10.4%. Application carbosulfan, phosphamidon are processed the nematode population 48.7%, 46.2% and 40.8%, 40.0% that can reduce respectively soil and latent root.The utilization that presses down line plant and fungus presses down line plant: the plant that foreign study finds to prevent and treat nematodiasis nearly 41 belongs to 47 kinds, has screened multiplely to the effective fungal metabolite of nematode, domesticly also pick up the ears (Pleurotusostreatus), shaggy cap and macro fungi kill the report of line activity. But be all limited to the result of study under the in vitro state of nematode in laboratory.
Plant source active component is different from the agricultural chemicals of chemical synthesis, it derives from plant, Environmental compatibility is good, can not cause new pressure to environment, the main performance that the agricultural chemicals of its existing chemical synthesis has, the advantage that has again chemical synthetic pesticide not have, it can not cause new pollution to environment in the whole ecosystem simultaneously. China's plant resources is abundant, and development botanical pesticide has resources advantage.
Summary of the invention
The object of the present invention is to provide plant source Rice-root Nematode Disease In Hunan Province prevention and control agent, by using this prevention and control agent, but can kill nematode or strengthen the tolerance of paddy rice to the root eelworm harm of diving, alleviate the harm of root nematode to paddy rice of diving, reach the object of increasing both production and income.
Of the present invention being characterized as: a kind of material as nematode prevention and control agent be chuanxiong (LigusticumchuanxiongHort) extract, said Rhizoma Chuanxiong extract is that Ligusticum wallichii obtains by lixiviate or extraction, lixiviate or extraction adopt water or organic solvent to do entrainer, wherein water can be Natural Water, distilled water etc., and organic solvent can be one or more in methyl alcohol, ethanol, propyl alcohol, butanols, amylalcohol, benzinum, chloroform, ethyl acetate, acetone. Extracting method is undertaken by following method: (1) material selection is dried Ligusticum wallichii root, and low-temperature grinding is crossed 20～50 mesh sieves; (2) Ligusticum wallichii after pulverizing is carried out to lixiviate or extraction, taking organic solvent as entrainer, as ethanol, its use amount is 10～20 times of powder total amount, and by reducing pressure, 45 DEG C of water-baths concentrate to obtain extract; Taking water as entrainer, as distilled water, its use amount is 15～30 times of powder total amount, and by reducing pressure, 60 DEG C of water-baths concentrate to obtain extract;
The method of the latent root nematode of prevention and control to rice hazard, is the prevention and control agent as the root nematode of diving with Rhizoma Chuanxiong extract, improves the tolerance of paddy rice to the root eelworm harm of diving.
Rhizoma Chuanxiong extract is 1g～5Og/m at amount of application while sprinkling2Time to rice safety, while being stained with seedling root in the time that amount of application is 1g～3Og/kg to rice safety.
Described Rhizoma Chuanxiong extract comprises the composition pesticide that itself and agricultural chemicals or Fertilizer Combination are made, and Rhizoma Chuanxiong extract and other auxiliary agents are made preparation.
The extract of Ligusticum wallichii is directly sprayed on rice leaf or cane after dilution; Or directly in the time of shoot transplanting equipment, be stained with seedling root.
The preparation that contains Rhizoma Chuanxiong extract and auxiliary agent, or contain described Rhizoma Chuanxiong extract and the composite rear composition sprayed of other agriculture chemicals on paddy rice; Or in the time of shoot transplanting equipment, be stained with seedling root.
The Rhizoma Chuanxiong extract energy prevention and control harm of root nematode to paddy rice of diving, taking ethanol extract as example, in pot experiment, when the root nematode of diving connects worm amount and is 100 heads/plant, amount of application is 1,5,10,20,30g/m2Rhizoma Chuanxiong extract is respectively 0.5%, 1.2%, 2.5%, 3.3%, 5.8% to the synergistic effect of rice yield.
The Rhizoma Chuanxiong extract energy prevention and control injury of root nematode to paddy rice of diving, taking distilled water extract as example, in field test, when the root nematode insect population radix of diving is 3400/100 grams of roots, amount of application is 1,5,10,20,30,40,50g/m2Rhizoma Chuanxiong extract is respectively 14.2%, 22.7%, 35.9%, 49.3%, 56.4%, 62.0%, 62.5% to the preventive effect of Hirschmanniella Oryzae, and the facilitation of rice yield is respectively to 0.3%, 0.3%, 1.1%, 2.4%, 8.0%, 5.6%, 5.4%.
Rhizoma Chuanxiong extract can reduce the infection rate of root nematode to paddy rice of diving, and taking ethanol extract as example, in pot experiment, when the root nematode of diving connects worm amount and is 200 heads/plant, amount of application is 1,5,10,15,20,25,30g/m2Rhizoma Chuanxiong extract, when results with do not execute extract comparison, insect population decline rate is respectively 22.0%, 33.8%, 41.7%, 56.9%, 62.5%, 68.8%, 72.4%.
Advantage and disadvantage of the present invention: obvious effect of increasing production, in a large amount of greenhouses, biological activity determination shows, and the root nematode of diving is to paddy rice generation harm, and after Rhizoma Chuanxiong extract is processed paddy rice, the obviously harm of the latent root nematode of prevention and control to paddy rice, has obvious effect of increasing production. After Rhizoma Chuanxiong extract is processed paddy rice, can improve the anti-infectivity of paddy rice to the root nematode of diving, invade the quantity in rice root thereby reduce the root nematode of diving. Ligusticum wallichii belongs to vegetable material, and good with Environmental compatibility, its extract is applied to paddy rice, to rice safety, and can be to environment yet.
Detailed description of the invention
Embodiment 1
Rhizoma Chuanxiong extract improves paddy rice to the anti-infectivity of root nematode of diving. The following examples are to further illustrate of the present invention, are below the results of indoor bioassay, but should not regard the restriction of by any way range of application of the present invention being carried out as in any case.
Select dry Ligusticum wallichii root, low-temperature grinding, crosses 20～50 mesh sieves; Ligusticum wallichii after pulverizing is carried out to lixiviate or extraction, and taking organic solvent as entrainer, as ethanol, its use amount is 10 times of powder total amount, and by reducing pressure, 45 DEG C of water-baths concentrate to obtain extract; (about 10g Ligusticum wallichii can obtain 3g extract), 4 DEG C of Refrigerator stores.
Load onto 200g wind desiceted soil at the plastic casing of 13cm × 8cm × 5cm, add 100ml Natural Water, at 25 DEG C of rice paddy seeds, soak seed 24 hours, vernalization 12 hours, chooses seed of the same size and is sowed in plastic casing after showing money or valuables one carries unintentionally at 30 DEG C, 15, every box, be placed in 25 DEG C of temperature daytimes, at 22 DEG C of nights, in the illumination box that intensity of illumination is 2500Lux, cultivate, day-night cycle is 12 hours, inoculates processing until paddy rice two leaves wholeheartedly time.
By dive 1500 of root nematodes of every box inoculation, then by Rhizoma Chuanxiong extract according to 1,5,10,20,30g/m2Five consumptions are processed respectively, and each processing 4 times repeats, and complete blank and inoculation contrast are set, and continue, according to above-mentioned condition illumination cultivation 15 days, to get root and leak worm. Rhizoma Chuanxiong extract improves paddy rice to the anti-infectivity of root nematode of diving. Result is as shown in table 1.
Table 1 Rhizoma Chuanxiong extract improves paddy rice to the anti-infectivity of root nematode of diving
In upper table, data are to repeat mean value four times
Embodiment 2
The land for growing field crops evaluating drug effect of the latent root nematode of Rhizoma Chuanxiong extract prevention and control to rice hazard.
Extracting method: select dry Ligusticum wallichii root, low-temperature grinding, crosses 20～50 mesh sieves; Ligusticum wallichii after pulverizing is carried out to lixiviate or extraction, taking water as entrainer, as distilled water, its use amount is 20 times of powder total amount, filter by 3 lixiviates of 60 DEG C of water-baths of reducing pressure, after collection concentrating filter liquor, obtain extract (about 20g Ligusticum wallichii can obtain 5g extract), 4 DEG C of Refrigerator stores.
Rice varieties: Lou You 305
The experimental plot root nematode radix of diving: 3400/100 grams roots
Paddy rice is on June 20th, 2013 sowing seedling, treats that rice shoot grew to for four leaf phases and transplants, and plants and within latter 10 days, carries out chemicals treatment, and processing method is that rice shoot is carried out to even spraying, and amount of application is 1,5,10,20,30,40,50g/m2Seven dosage, contrast medicament A Wei 1.8%EC0.2ml/m2, repeat, not dispenser blank be set for 4 times; After chemicals treatment, worm is leaked in sampling in 40 days, when results, surveys and produces.
The land for growing field crops evaluating drug effect of the latent root nematode of table 2 Rhizoma Chuanxiong extract prevention and control to rice hazard
In upper table, data are to repeat mean value, output and worm amount round number four times.
Embodiment 3
The land for growing field crops evaluating drug effect of the latent root nematode of Rhizoma Chuanxiong extract prevention and control to rice hazard.
Extracting method: Ligusticum wallichii root drying, low-temperature grinding, crosses 20～50 mesh sieves; Ligusticum wallichii after pulverizing is carried out to lixiviate or extraction, and taking organic solvent as entrainer, as acetone, its use amount is 10 times of powder total amount, filters by 3 lixiviates of 40 DEG C of water-baths of reducing pressure, and after collection concentrating filter liquor, rotary evaporation obtains extract; (about 12g Ligusticum wallichii can obtain 1g extract), 4 DEG C of Refrigerator stores.
Rice varieties: Lou You 36
The experimental plot root nematode radix of diving: 3920/100 grams roots
Paddy rice is in sowing seedling on April 15th, 2014, treat that rice shoot grows to the transplanting of four leaf phases, when transplanting, carry out chemicals treatment, processing method is that rice shoot root is stained with to root processing, with being stained with seedling root containing the medicine fertilizer of Rhizoma Chuanxiong extract 5,10,15,20,25,30g/kg, contrast medicament, containing A Wei 0.1g/kg, arranges not dispenser blank; Within after transplanting 3,7,14,20 days, observe seedling growth situation, within 45 days, sample afterwards and leak worm.
Seedling growth situation observed result shows, extract is stained with root seedling growth is had no adverse effects.
Leak worm result and show, be stained with root processing and can obviously reduce nematode intrusion amount, 15g/kg processes with to contrast pharmacy effect suitable.
Claims (3)
1. one kind is utilized Rhizoma Chuanxiong extract to alleviate the method for latent root nematode to rice hazard, it is characterized in that: after said Rhizoma Chuanxiong extract dilution, be directly sprayed on the rice leaf and cane in various growth periods, or be sprayed on rice leaf and cane after Rhizoma Chuanxiong extract and fertilizer or pesticide combination, Rhizoma Chuanxiong extract is 1～50g/m at amount of application2Time to rice safety.
2. one kind is utilized Rhizoma Chuanxiong extract to alleviate the method for latent root nematode to rice hazard, it is characterized in that: the rice shoot directly applying to while transplanting after said Rhizoma Chuanxiong extract dilution is stained with root, or after Rhizoma Chuanxiong extract and fertilizer or pesticide combination, in the time of shoot transplanting equipment, be stained with root, Rhizoma Chuanxiong extract in the time that amount of application is 1～30g/kg to rice shoot safety.
3. a kind of Rhizoma Chuanxiong extract that utilizes according to claim 1 alleviates the method for root nematode to rice hazard of diving, and it is characterized in that: described latent root nematode comprise Hirschmanniella (Hirschmanniellaspp) in one or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410639378.6A CN105594752A (en) | 2014-11-14 | 2014-11-14 | Method for preventing and controlling rice Hirschmanniella oryzae diseases by using plant material ligusticum chuanxiong hort |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410639378.6A CN105594752A (en) | 2014-11-14 | 2014-11-14 | Method for preventing and controlling rice Hirschmanniella oryzae diseases by using plant material ligusticum chuanxiong hort |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105594752A true CN105594752A (en) | 2016-05-25 |
Family
ID=55975565
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410639378.6A Pending CN105594752A (en) | 2014-11-14 | 2014-11-14 | Method for preventing and controlling rice Hirschmanniella oryzae diseases by using plant material ligusticum chuanxiong hort |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105594752A (en) |
-
2014
- 2014-11-14 CN CN201410639378.6A patent/CN105594752A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Manh et al. | Golden camellias: A review | |
| CN101524067A (en) | Usage of botanical molluscicide and tea saponin thereof | |
| CN106490008A (en) | A kind of agricultural chemicals of preventing and treating fruit tree diseases and pests and preparation method thereof | |
| CN102204576A (en) | Method for relieving damage of hirschmanniella oryzae to rice by using Agrimonia pilosa Ledeb. extract | |
| KR100555077B1 (en) | Ferment solution of plants and method for fertilizer application or killing insects using the solution | |
| CN107593245A (en) | Oil tea pest and disease damage integrates effective administering method | |
| Wakjira | Allelopathic effects of Parthenium hysterophorus L. on germination and growth of onion. | |
| Nishanthan et al. | Seedbank dynamics and integrated management of Parthenium hysterophorus in vegetable capsicum | |
| CN102726461A (en) | Method of mitigating harm of Hirschmanniella to paddy rice by using extract product of Perilla frutescens (L.) Britt | |
| Hossain et al. | Enhancing seed germination and seedling growth attributes of a medicinal tree species Terminalia chebula through depulping of fruits and soaking the seeds in water | |
| CN106472591B (en) | Ecological insecticidal bactericide and preparation method thereof | |
| Nishanthan et al. | Control of Parthenium hysterophorus L. and its impact on yield performance of tomato (Solanum lycopersicum L.) in the northern province of Sri Lanka. | |
| CN107466656A (en) | A kind of method for integrated control of soil-borne disease for pepper cultivation | |
| CN106495862A (en) | A kind of composite pesticide for apple tree cultivation and preparation method thereof | |
| Bala et al. | Field evaluation of chilli germplasms against yellow mite, Polyphagotarsonemus latus (Banks)(Acari-Tarsonemidae) and its management under gangetic basin of West Bengal. | |
| CN106561646A (en) | A method for relieving poisoning effects of amide-type herbicides to rice by utilizing a zanthoxylum bungeanum extract product | |
| CN105594752A (en) | Method for preventing and controlling rice Hirschmanniella oryzae diseases by using plant material ligusticum chuanxiong hort | |
| CN105009926A (en) | Method for preventing insects, retaining water and increasing yield by covering gardenia garden with straw soaked with Chinaberry seed solution | |
| Kwembeya et al. | Allelopathic effects of lantana (Lantana camara) on blackjack (Bidens pilosa) and pearl millet (Pennisetum glaucum) | |
| Prakash et al. | Cumulative effect of botanical seed pelleting and foliar spray on morpho physiological, leaf chlorophyll, gas exchange and yield parameters in black gram | |
| Homez et al. | Evaluation of soil temperature under mulches and garlic extract on yield of cucumber (Cucumis sativus L.) in greenhouse conditions | |
| CN103609602A (en) | Method for relieving damage of rice root nematode on rice by using artemisiifolia extract | |
| Kumar et al. | Tree—crop interactions in the agroforestry system of Mizoram | |
| CN104663735A (en) | Method for reducing harm of Hirschmanniella spp on rice by using Xanthium sibiricum Patr extract | |
| Butola et al. | Technology for propagation and cultivation of Angelica glauca Edgew.: a threatened high value Himalayan medicinal cum edible herb |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160525 |
|
| WD01 | Invention patent application deemed withdrawn after publication |