KR20230023871A - Composition of acaricide with nature plant extract - Google Patents

Abstract

The present invention relates to an acaricide composition comprising a mixture of catnip oil and a ginkgo leaf extract as an active ingredient. The acaricide composition of the present invention exhibits an increased acaricidal effect compared to commercially available concentrations of d-phenothrin, includes the composition of supplements that can enhance the acaricidal effect of natural extracts in an optimal condition, and uses natural extract ingredients that exclude organic chemicals, and thus is eco-friendly and economical.

Description

Acaricide composition using natural plant extract {Composition of acaricide with nature plant extract}

The present invention relates to an acaricide composition using a natural plant extract, and more particularly, to an acaricide composition comprising a mixture of catnip oil and ginkgo leaf extract as an active ingredient.

House dust mite is one of the mites distributed all over the world, and lives by eating organic matter such as human skin cells or mold in damp corners of houses, bedding, chairs, carpets, etc. House dust mites can cause dermatitis, but more than that, they are also the main cause of allergic diseases represented by asthma.

Among the house dust mites (Family Pyroglyphidae), the large-legged dust mite ( Dermatophagoides farinae ) is the main species in the United States, and the vertical pattern dust mite ( Dermatophagoides pteronyssinus ) is the main species in the Old World, such as Europe, and both of these species are known to exist in Korea. They cause allergies by exposing people to concentrated antigens. In the United States, 4% of the general population is known to be allergic to this tick allergen.

On the other hand, currently marketed mite inhibitors are manufactured using toxic synthetic mite repellent materials (d-phenothrine, permethrin) for reasons such as manufacturing cost, manufacturing convenience, and availability of raw materials. These components act as the main cause of skin troubles apart from allergies caused by house dust mites, and due to these side effects, these components are inappropriate to be added to the mite repellent composition.

Moreover, if the active ingredient responsible for the mite repellent effect causes skin irritation, it may be difficult to develop a product and use it at a high concentration, lowering the value of the product. It is more vulnerable to damage, and care must be taken in its handling, such as requiring more attention to the use of existing irritant mite inhibitors.

Korean Patent Registration No. 10-1827982 (2018.02.05) discloses a chicken mite acaricide composition using natural plant extracts. Korean Patent Publication No. 10-2016-0122808 (2016.10.24) discloses a fungicidal composition of pyrazole carboxylic acid alkoxyamide.

An object of the present invention is to provide a household acaricidal composition using extracts derived from natural plants excluding organic compounds.

The present invention provides an acaricide composition comprising Nepeta cataria oil and Ginkgo biloba leaf extract.

In the acaricidal composition of the present invention, the catnip oil and the ginkgo leaf extract are preferably included in an amount of 1 to 10% by weight, respectively, based on the total content of the acaricidal composition.

In the acaricidal composition of the present invention, the ginkgo biloba extract is preferably a supernatant of an extract obtained by using water or an aqueous ethanol solution as an extraction solvent.

The acaricidal composition of the present invention preferably has an acaricidal effect against any one or more selected from house dust mites, small cattle mites, hair mites, and ear mites.

The acaricidal composition of the present invention, for example, disodium EDTA, citric acid, sodium citrate, butylene glycol, glycerin, 1,2-hexane Diol (1,2-Hexanediol), Acrylates/C10-30 Alkyl Acrylate Crosspolymer (Acrylates/C10-30 Alkyl Acrylate Crosspolymer) aqueous solution, hydroxyethylcellulose (Hydroxyethylcellulose) aqueous solution, and triethanolimine (Triethanolamine) aqueous solution are further included it may be

Meanwhile, the present invention is catnip (Nepeta cataria) oil and ginkgo biloba (Ginkgo biloba) extract, Disodium EDTA, citric acid (Citric Acid), sodium citrate (Sodium Citrate), butylene glycol (Butylene Glycol), Glycerin, 1,2-hexanediol, Acrylates/C10-30 Alkyl Acrylate Crosspolymer aqueous solution, Hydroxyethylcellulose aqueous solution and a salbi sheet containing an aqueous solution of triethanolamine.

The acaricidal composition of the present invention shows an increased acaricidal effect compared to commercially available d-phenothrine, and is a natural extract component excluding organic chemicals by including an auxiliary composition capable of enhancing the acaricidal effect of a natural extract under optimal conditions. , eco-friendly and economical.

As described above, conventional methods for killing house dust mites at home often use chemicals or substitute organic synthetic insecticides, and harmful chemical components may remain in bedding or clothing used at home. It can adversely affect the health of people located in close living environments.

In particular, most of the recent mite repellents manufactured by domestic pharmaceutical companies are selling products made with ingredients such as d-phenothrine, diethyltoluamide, and paramenthane-3,8-diol, but natural extracts As a result, there is no product developed as a low-toxic formulation to the human body, and the development of products made of natural extracts that are safer, more environmentally friendly, and have good efficacy is required.

Accordingly, the present inventors have tried to develop an acaricidal composition for killing house dust mites in a more environmentally friendly and economical way at home. As a result, catnip (Nepeta cataria) oil and Ginkgo biloba leaf extract have acaricidal activity By confirming that it can be effectively used as an acaricidal composition against house dust mites, the present invention was completed.

Accordingly, the present invention provides an acaricide composition comprising Nepeta cataria oil and Ginkgo biloba leaf extract. At this time, the catnip oil and ginkgo leaf extract are preferably included in 1 to 10% by weight, respectively, based on the total content of the acaricidal composition. This is because the effect of increasing the acaricidal activity compared to the amount added is insignificant and is not economical.

Catnip (Nepeta cataria) is also called catnip or stone mint. Dicotyledonous plant It is a perennial plant of the Lamiaceae family that grows in mountains and fields. Because of the ingredient called 'Nepetalactone' in catnip, cats look like they are drunk the moment they come into contact with it. In addition, according to a study by Iowa State University in the United States, there is also a result that mosquitoes avoid catnip because it has some degree of insect repellent effect.

Ginkgo is a deciduous tree that belongs to gymnosperms and is planted as a garden or street tree. The stem is straight, and the branches grow obliquely toward the top, reaching a height of 20 to 35 m. Fan-shaped leaves turn yellow in autumn. It is resistant to various diseases and insects and lives for a long time. The leaves have antibacterial components such as flavonoids, so there is no harm from pests, and the fruit has hemtinic acid, which gives off a bad smell, and has ginchoric acid, which causes dermatitis. If you use ginkgo leaves as a bookmark, mold is prevented by the insect repellent action of butyric acid.

For the ginkgo leaf extract of the present invention, conventional methods in the art such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction may be used, and the methods are not limited.

On the other hand, catnip (Nepeta cataria) oil and Ginkgo biloba leaf extract are widely used in the cosmetic and pharmaceutical markets, and their safety has been proven, and as natural products, they have the advantage of being able to approach consumers as safer products.

In addition, the present inventors confirmed in one embodiment of the present invention that the acaricidal composition of the present invention exhibits significant acaricidal efficacy compared to commercially available acaricide, commercially available concentration (0.003 g in 100 cm ² ) of d-petotrin, and is environmentally friendly and economical. there is.

On the other hand, in the acaricidal composition of the present invention, the ginkgo leaf extract is preferably extracted using water, C1 to C4 lower alcohol, or a mixture thereof as a solvent, more preferably water or ethanol aqueous solution as the extraction solvent Preferably, the supernatant of the extract obtained by using As a result of preparing the ginkgo biloba extract using various extraction solvents, the present inventors confirmed the excellent acaricidal effect of the ginkgo biloba extract when water or ethanol aqueous solution was used as the extraction solvent.

The acaricidal composition of the present invention preferably has an acaricidal effect against any one or more selected from house dust mites, small cattle mites, hair mites, and ear mites.

House dust mites are animals belonging to the spider class (spiders, mites, ticks, etc.), and are very small with a size of 0.1 to 0.3 mm. It mainly eats dead skin cells, plant fibers, dust in the house, and mold spores. Dust mites do not bite or sting, and do not spread disease themselves, so they are completely harmless in themselves. However, protein components contained in excrement and remains of house dust mites cause allergic symptoms when they come into contact with human skin or enter the respiratory tract.

The house dust mite becomes a larval mite after hatching from an egg and becomes the first juvenile mite without a blood sucking process. The first juvenile mite becomes a second juvenile mite within 24 hours of bloodsucking, and then further bloodsucking to become a mature mite. It becomes a mature mite in about 9 days from an egg, and repeats blood-sucking and egg-laying.

On the other hand, the body of the small socham tick is divided into a front part with a mouthpiece (mouth) and a small head, and a rear part with legs, digestive tract, and reproductive organs. Both males and females are yellowish brown or greenish brown, have no antennae, compound eyes, or wings, and have four pairs of walking legs like other spiders, a Y-shaped anus on the back, and a fourth trachea, a breathing hole. It's behind the bridge. In the larval stage, it has three pairs of legs, but as it molts and becomes a nymph, it becomes four pairs.

In addition, the small sorceress tick lives in Korea, Japan, China, Russia, Australia, New Zealand, etc., and is distributed nationwide, including Jeju Island, Gyeonggi-do, and Gangwon-do in Korea. It may have existed for a long time, but I didn't know what it was, and in 2011, this mite mediated phlebovirus (an RNA virus belonging to the genus Phlebovirus) and caused SFTS (Severe Fever with Thrombocytopenia Syndrome). It became known that

On the other hand, the family Trombidiidae is a group of mites including free-living or parasitic types. Parasitic species are parasitic only during the larval stage, and there are nymphs and free-living adults. Of medical concern are species belonging to the subfamily Trombiculinae that parasitize humans and mammals in the larval stage. Several genera are distributed in Korea, and it is observed with high frequency in field mice. There are many patients in Japan.

Damage caused by hair mites is divided into skin damage caused by larvae and infections caused by Rickettsia (tsutsugamushi disease). When bitten by a larva, tissue is damaged, and small red papules form over the course of a day due to stimulation by saliva. It is very itchy, and scratching causes secondary infection. It can also lead to fever and nervous system poisoning. It can occur on any part of exposed skin, and first-time bites are more severe. People who have been repeatedly bitten have weak symptoms, and people who have developed immunity are less likely to be bitten.

On the other hand, Tsutsugamushi disease is an acute rash disease of the typhus group (scrub typhus, tsutsugamushi disease) caused by infection with Orientia tsutsugamushi , a Rickettsia mediated by hair mites. In Korea, hundreds to thousands of patients are concentrated in the fall each year. This pathogen was isolated from Leptotrombidium pallidum , which is parasitic on wild rat rats in Korea. In Japan, reed vole is the main host and Trombicula akamushi is known as a vector.

Ear mites, on the other hand, are large and round in shape, resembling coffee grounds. Ear mites live on the surface of the skin, but are not buried like mange mites. Ear mites are a major cause of ear infections in many dogs and are known to have a high incidence. Ear mites are spread by direct contact, easily infected by contact between animals, and are often found in environments where several puppies are raised together.

On the other hand, the acaricidal composition of the present invention, for example, disodium EDTA, citric acid, sodium citrate, butylene glycol, glycerin, 1,2 -Hexanediol (1,2-Hexanediol), Acrylates/C10-30 Alkyl Acrylate Crosspolymer (Acrylates/C10-30 Alkyl Acrylate Crosspolymer) aqueous solution, hydroxyethylcellulose (Hydroxyethylcellulose) aqueous solution and triethanolimine (Triethanolamine) aqueous solution It may further include, and provides a salbi sheet containing the same. Each of the above compounds may be included in an amount of 1 to 10% by weight.

In one embodiment of the present invention, the present inventors used catnip oil and ginkgo leaf extract as a house dust mite acaricidal composition, and selected optimum conditions for sheet paper application that can increase the effect, and were able to specify the above combination.

Disodium EDTA is a white crystalline granule or a white-grey-white powder that has no odor. Its chemical formula is C ₁₀ H ₁₄ N ₂ Na _2O8 .2H ₂ O, and it is mainly used as an antioxidant. It is soluble in water and insoluble in organic solvents such as ethanol. Stable in air. EDTA is a well-known chelating agent that combines with various metal ions to form complex salts, and is very stable compared to other chelating agents. Usually, as the pH increases, EDTA dissociates better and forms more complex compounds with metal ions. Complex formation of calcium and EDTA is best formed at pH 8.5 or higher, and zinc is well formed at pH 6.0. However, EDTA does not form a complex by combining with calcium at a pH below 3.0.

Citric acid is one of the polybasic carboxylic acids having a hydroxyl group, and is a weak organic acid contained in many citrus fruits and vegetables. It is most abundant in lemons and limes and accounts for 8% of the dry weight of the fruit. It is also used as a preservative to stabilize medicines and as a disinfectant against viruses and bacteria.

Sodium citrate is composed of the chemical formula Na ₃ C ₆ H ₅ O ₇ ㆍ2H ₂ O and shows colorless monoclinic columnar crystals. It is stable in air and becomes anhydrous at 150 °C. Sodium citrate is used as a pH buffer, acidity regulator or sequestrant.

Butylene glycol (Butylene Glycol) is a component made from substances derived mainly from fatty acids extracted by fermenting sugar cane. Also called butylene glycol. It is mainly used as a moisturizer, skin conditioning agent, solvent, viscosity reducing agent, and fragrance.

Glycerin is a sugar alcohol and has three hydrophilic alcohol hydroxyl groups (OH-) involved in water solubility. Glycerin is an important component of triglycerides (ie, fats and oils) and phospholipids. The pattern of use of glycerol is extensive and can be found in industrial, professional and consumer products. Glycerol is used as an ingredient in many products and as an industrial intermediate for products such as soaps and detergents and for the manufacture of glycerol esters. It is found in consumer products such as pharmaceuticals, cosmetics, tobacco, food and beverages, and in many other products such as paints, resins and paper.

1,2-Hexanediol has a structure of a surfactant that has both hydrophobicity and hydrophilicity, so it mixes well with oil and water, so it does not change the properties of the product and is a substitute for preservatives. It is also used as a natural preservative.

The raw material of Acrylates/C10-30 Alkyl Acrylate Crosspolymer is one or more simple esters or methacrylic acid crosslinked with allyl ethers of sucrose or allyl ethers of pentaerythritol; It is an archpolymer of one or more monomers composed of one of methacrylic acid and C10-30 alkyl acrylate. It is mainly used as a stabilizer for unstable emulsions, a viscosity modifier, and a film forming agent.

Hydroxyethylcellulose is a water-soluble polymer obtained by reacting cellulose with ethylene oxide to neutralize, purify, dry, and crush alkali cellulose by reacting caustic soda with cellulose. It is widely used as a stabilizer and thickener for emulsifying and dispersing products, shampoos, rinses and lotions.

Triethanolamine is an oily liquid with a slight ammonia odor. It is an organic compound that is denser than water, has a freezing point of 71°F, and is both a tertiary amine and a trialcohol. It is mainly used as a pH balance maintaining material and emulsifier.

Hereinafter, the contents of the present invention will be described in more detail through the following examples or experimental examples. However, the scope of the present invention is not limited only to the following examples or experimental examples, and includes modifications of equivalent technical ideas.

[Example 1: Preparation of the plant extract of the present invention]

For the preparation of the plant extract of the present invention, commercially available catnip oil (appalachian valley natural products, Catnip essential oil) was used.

On the other hand, the extract of ginkgo leaves (Gingko) was collected after pulverizing the ginkgo leaves in a grinder, and 40 g of the ginkgo leaves was immersed in 200 ml of an extraction solvent in an extraction container and extracted at room temperature for 7 days. At this time, 50% ethanol aqueous solution was used as the extraction solvent.

Thereafter, the extract was obtained by removing the debris using a sieve, and the supernatant was recovered and obtained by additional centrifugation, and stored in a refrigerator for the salbi test.

[Example 2: Preparation of house dust mite acaricidal composition for sheet paper containing catnip oil and ginkgo leaf extract]

For the preparation of acaricide for sheet paper, ginkgo leaf extract and catnip oil obtained in Example 1, Disodium EDTA, Citric Acid, Sodium Citrate, Butylene Glycol , Glycerin and 1,2-hexanediol were put into a mixer and mixed for 30 minutes to prepare an aqueous layer.

Then, an aqueous solution of Acrylates/C10-30 Alkyl Acrylate Crosspolymer, an aqueous solution of hydroxyethylcellulose, and an aqueous solution of triethanolamine were sequentially added, followed by 30 minutes It was emulsified to prepare a gel-type acaricidal composition.

The detailed composition of the acaricidal composition is shown in Table 1 below.

Composition of house dust mite acaricidal composition containing catnip oil and ginkgo leaf extract Product Dust Mite Control Agent Name Phase ingradiant wt% A Water 58.55 ginkgo leaf extract 7 catnip oil 3 Disodium EDTA 0.05 Citric Acid 0.05 Sodium Citrate 0.15 Butylene Glycol 7 Glycerin 5 1,2-Hexanediol 2 B Water 9.8 Acrylates/C10-30 Alkyl Acrylate Crosspolymer 0.2 C Water 4.9 Hydroxyethylcellulose 0.1 D Water 2 Triethanolamine 0.2 Total 100

[Example 3: Manufacturing sheet paper using the house dust mite acaricidal composition of Example 2]

For the preparation of the house dust mite acaricidal composition sheet, 5 g of the composition prepared above was applied per felt sheet (22.5 cm × 15 cm) and naturally dried for 24 hours.

Then, it was used in Experimental Example 1 to evaluate the acaricidal efficacy.

[Experimental Example 1: Evaluation of acaricidal efficacy of the house dust mite acaricidal sheet of the present invention]

In order to evaluate the acaricidal efficacy of the acaricidal sheet prepared in the present invention against house dust mites, the degree of death of house dust mites was confirmed by a residue contact test method (continuous contact test method). For convenience of notation, the salbi sheet prepared in the present invention is referred to as a 'test sheet' in the following.

1. Residue contact test method (continuous contact test method)

① A test sheet was cut and pasted to the size of the bottom of a Petri dish (diameter 5 cm), and active adult dust mites were checked under a dissecting microscope, and 25 of them were placed with an art brush.

② Petri dishes (5cm) were prepared in the same way for both the negative control group and the positive control group, dust mites were put there, and dead individuals were observed after 24 hours.

③ In the center of the Petri dish lid, there is a fine mesh with a diameter of 1.5 cm to allow air to pass through the inside and outside of the Petri dish.

④ The temperature of 26 ~ 28 ℃ and humidity of 65 ~ 70% were maintained in the test and storage areas, and the test group and control group were stored separately so that they were not affected by each other.

⑤ The test was repeated three times, and the mortality rate was calculated by observing the dead individuals after 24 hours.

2. Negative control group

0.1 ml of 50% ethanol (based on 50 ml/m2) was dropped on a filter paper with a diameter of 5 cm, dried for about 15 minutes, attached to the bottom of a petri dish (5 cm in diameter), and 25 dust mites were placed there.

3. Positive control group

The sheet was prepared so that the concentration of d-phenothrin was 0.003 g in 100 cm ² , the same as the concentration of commercially available house dust mite eradication sheet products containing d-phenothrin, in the negative control group. Dust mites were exposed in the same way as the treatment method.

4. Experimental results

The experimental results confirmed two things: the result of confirming the acaricidal effect on dust mites after 24 hours of exposure and the result of confirming the acaricidal effect of dust mites according to the elapsed time (days) after opening the test sheet.

First, the results of confirming the acaricidal effect on dust mites after 24 hours of exposure are shown in Table 2 below.

Acaricidal effect on large-legged dust mites of the test sheet test group Mean mortality (%) ± SD after 24 hours exposure division negative control group positive control test sheet Total test population 75 75 75 total lethal population 0 4 75 Mean mortality (%)±SD 0±0 5.3±6.1 100±0

As a result of the experiment, as shown in Table 2, the acaricidal sheet of the present invention showed 100% acaricidal activity against house dust mites after 24 hours of exposure, which was superior to that of the positive control d-petothrin-containing sheet (about 5%). It was confirmed to have.

On the other hand, the results of confirming the acaricidal effect of dust mites according to the elapsed time (days) after opening the test sheet are shown in Table 3 below.

Acaricidal effect of large-legged dust mite according to the elapsed time (days) after opening the test sheet test group Mean mortality (%) ± SD over elapsed time (days) division 10 20 30 40 50 60 70 80 90 100 negative control group 0±0 0±0 0±0 0±0 0±0 0±0 0±0 0±0 0±0 0±0 positive control 8.0±4.0 4.0±4.0 5.3±4.6 5.3±2.3 8.0±4.0 5.3±4.6 2.7±2.3 4.0±4.0 1.3±2.3 1.3±2.3 test sheet 100±0 100±0 100±0 100±0 98.7±2.3 98.7±2.3 96.0±0 89.3±2.3 80.0±4.0 68.0±8.0

As a result of the experiment, as shown in Table 3, the acaricide sheet of the present invention maintained 98% or more of acaricidal activity against house dust mites even after 60 days of manufacture, confirming that it was very economical.

Claims (6)

An acaricide composition comprising Nepeta cataria oil and Ginkgo biloba leaf extract.
According to claim 1,
The catnip oil and ginkgo leaf extract,
An acaricidal composition, characterized in that it is included in 1 to 10% by weight based on the total content of the acaricidal composition.
According to claim 1,
The ginkgo leaf extract,
An acaricidal composition, characterized in that it is a supernatant of an extract obtained by using water or an aqueous ethanol solution as an extraction solvent.
According to claim 1,
The acaricidal composition,
An acaricidal composition characterized in that it has an acaricidal effect against any one or more selected from house dust mites, small cattle mites, hair mites and ear mites.
According to claim 1,
The acaricidal composition,
Disodium EDTA, Citric Acid, Sodium Citrate, Butylene Glycol, Glycerin, 1,2-Hexanediol, Acrylates / C10-30 Alkyl Acrylate Crosspolymer (Acrylates / C10-30 Alkyl Acrylate Crosspolymer) aqueous solution, hydroxyethylcellulose (Hydroxyethylcellulose) aqueous solution and triethanolimine (Triethanolamine) aqueous solution characterized in that it further comprises an acaricidal composition.
An acaricidal sheet containing the acaricidal composition of claim 5.