KR101400713B1 - Composition of pesticide containing pesticidal essential oils and/or food additives - Google Patents

Abstract

The present invention relates to insecticidal (non) compositions comprising plant essential oils and / or food additives. They exhibit insecticidal (non-irritant) properties or exhibit their synergistic function when mixed with each other. Insecticides containing them provide the ability to safely kill endangered species.

Description

≪ Desc / Clms Page number 2 > < RTI ID = 0.0 > [0002] < / RTI > A composition for pesticidal use containing insoluble essential oils and /

The present invention relates to insecticides containing insecticidal essential oils and insecticidal compounds, and compositions capable of exerting a lift through mixing thereof. More particularly, the present invention relates to a composition for preventing the harmful organisms of compounds used in plant essential oils and food additives, It provides animal loyalty to animals. In particular, the plant essential oils and the insecticidal compounds of the present invention exhibit an enhanced ascending power upon mixing even at a concentration which does not exert insecticidal power alone, thereby providing strong insecticidal characteristics. It can be used as a means to effectively control pests acutely in a small amount and can be used to protect plants, livestock, pets, and humans.

As invertebrates, important pests have been competing with our human beings since history, and they have been causing problems such as agricultural productivity decrease in agriculture, disease mediation and induction of newness in hygiene health. As an effective means of preventing the decrease in agricultural productivity, pesticides are mainly dependent on the majority of countries in the world including Korea. However, due to excessive pesticide dependence, its use is severely limited due to the emergence of resistant organisms, toxicity to human body and environment. Much attention has been devoted to research and development to utilize plants as alternatives to these organic chemical syntheses. In this respect, various research and development attempts have been made on Korean Patent Nos. 0350391, 0391827, 0530840, 0486819, 0514978 and 0511823. However, most of these patents have a disadvantage in that they are able to inhibit harmful organisms only by using relatively high amounts because they are focused only on activity based on plant insecticidal (non). This is a very weak part in terms of production cost, and there was a limit to the attempt to develop actual products. Therefore, the present invention focuses on the synergistic action of plants and compounds capable of overcoming such weaknesses.

Generally, synergists do not show direct toxicity at the concentration (dose) used, but when used in combination with those exhibiting insecticidal (non-toxic) effects, they have the effect of enhancing their insecticidal power. Up to now, the most commercially successful ascertaining agent is piperonyl butoxide, which has been developed as a synergistic agent for pyrethroid insecticides. However, in recent years, the use of this synergist has been gradually losing ground, with the use of pyrethroid insecticides declining.

In recent years, many technologies for controlling harmful organisms using plants have been developed due to increased interest in environment and health. In particular, studies have been conducted to effectively control arthropods using plant essential oils, but their utilization rate is low due to their high price compared to chemical compounds and insufficient preparation of various data necessary for registration. Therefore, to overcome these weaknesses, reducing the production cost by reducing the use of insecticides through synergy can be an alternative. Studies to develop cheaper and environmentally friendly pest control agents with these approach alternatives are conducted through the present invention and are provided below.

It is an object of the present invention to provide plant essential oils that are insecticidal and environmentally safe while exhibiting insecticidal (non-toxic) power against various harmful organisms, especially arthropods.

It is another object of the present invention to provide insecticidal (non-toxic) power of compounds widely used as plant-derived or food additives.

The present invention also provides a synergistic feature of providing a strong insecticidal power by increasing the activity at the time of mixing even when there is no insecticidal power at a concentration (content) applied solely by mixing these plant essential oils and food additives with insecticidal It is another purpose.

In order to accomplish the above object, the present invention provides a method for producing an oil-in-water emulsion composition comprising an anise oil, a basil oil, a heistoyl oil, a terpentine oil, a pacolioil, a black pepper oil, an estradiol oil, a saborioil, , Methyl cinnamate, cinnamic acetate, methyl salicylate, estragol, 3-pinanone, phenethyl 2-methylbutyrate, phenethyl acetate, pen Phenethyl anthranilate, Phenethyl benzoate, Phenethyl butyrate, Phenethyl cinnamate, Phenethyl hexanoate, Phenethyl isobutyrate, But are not limited to, Phenethyl isobutyrate, Phenethyl isovalerate, Phenethyl octanoate, Phenethyl salicylate, Phenethyl tiglate, There is provided a fungicidal composition comprising at least one compound selected from the group consisting of isobutylphenethyl alcohol, methylphenethyl butyrate, phenethyl phenylacetate and the like.

The plant essential oils or insecticidal (non) compounds act synergistically to increase their insecticidal activity. Therefore, when at least one plant essential oil among the above-mentioned insecticidal compositions is selected and one or more kinds of insecticidal compounds are selected and mixed, they exhibit synergistic properties exhibiting insecticidal activity even in a range where they do not show insecticidal (non-exerted) power.

The plant essential oils and compounds of the present invention exhibit insecticidal or insecticidal activity against arthropods and preferably exert their functions against agricultural pests, hygiene pests, domestic pests, external parasites and the like.

The most representative agricultural pests are spotted mite, peach aphid, powdery mildew, thripworm, etc. Each of these insecticidal compositions can be excellent for managing resistant pests.

The most common sanitary or domestic pests are mosquitoes, house dust mites, hair mites, moth flies.

The most common animal parasites are chicken mites (chicken red mites) and ear mites.

Among the insecticidal (non-toxic) compositions of the present invention, one or more compounds selected from the group consisting of anise oil, basil oil, histo oil, terpentine oil, patcholioyl, black pepper oil, It is preferable that the number of species or more is selected. The insecticidal (non-toxic) composition may further comprise a substance contained in a conventional insecticide, wherein the composition may comprise at least one selected from the group consisting of 0.5-70% Preferably 2-10% by weight. However, it is a matter of course that the content of the plant essential oil contained in the insecticidal composition is appropriately adjusted according to the method of using the insecticide or the presented formulation.

The plant essential oil is preferably obtained by extraction such as steam distillation or hydrodistillation, supercritical extraction or the like. These essential oils, which exhibit insolubility, can also be obtained by means of combining them with their main constituents via combinatorial chemistry.

Among the insecticidal (non-toxic) compositions of the present invention, ethyl cinnamate, methyl cinnamate, cinnamyl acetate, methyl salicylate, estragol, 3-pinanone, phenethyl 2-methyl butyrate, phenethyl acetate, Phenethyl benzoate, phenethyl butyrate, phenethyl cinnamate, phenethyl hexanoate, phenethyl isobutyrate, phenethyl isobalate, phenethyl octanoate, phenethyl salicylate, pen Ethylhexyl acetate, ethyl tiglate, isobutylphenethyl alcohol, methylphenyl ethyl butyrate, phenethyl phenyl acetate, and the like. The insecticidal (non-toxic) composition may further comprise a substance contained in a conventional insecticide, wherein the composition may include at least one selected from the group, and the content thereof is preferably from 0.1 to 70% Preferably 0.5 to 10% by weight. However, it is a matter of course that the content of the compound contained in the insecticidal composition is appropriately adjusted according to the method of using the insecticide or the presented formulation.

The insecticidal (non-insoluble) composition may be formulated into a commercialized general insecticide formulation used for insecticidal purposes against a variety of insects including, for example, emulsions, emulsions, suspensions, solutions, dispersions, wettable powders, Capsules, suspensions, powders, fine powders, granules, granules, capsules, fumigants, fumigants and the like. In addition, aerosol, smoke, spray (pump type), and gel-like formulations are preferable for domestic use and anti-viral use, and more preferably, they can be manufactured from a liquid formulation.

Hereinafter, preferred examples for the understanding of the present invention will be described. However, the following experimental examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited to the following experimental examples.

As mentioned above, the present invention provides plants or compounds that are insecticidal (non-resistant) to agricultural pests, hygiene pests, domestic pests, external parasites. They also exhibit a synergistic effect which shows a marked increase in the insecticidal (non-exerted) power that can not be exhibited in the single treatment through intermixing. These characteristics mean that they will be used as environmentally friendly, low-toxicity natural insecticides in appropriate future combinations to improve agricultural productivity and to be used as a means of mediating disease of humans and animals. This aspect is a newly proven feature of the present invention.

[Experimental Example 1] Insecticidal (non) property against agricultural pests

Experimental materials were dissolved in 5 ml of ethanol, and 10 - 35 individual insect pests with excellent vigor were inoculated by preparing leaf slices (diameter 5 cm) of host plants of each insect pests. All experiments were done in 3 replicates.

The spotted mite used in the system was cultivated for more than 10 years without insecticide culling. The resistance system was pyridaben, an acaricide. Petri dishes (5.5 cm in diameter) were laid on the ground with a cotton wool cloth, and then the prepared kidney bean leaf slices were inoculated and 20 mice of the susceptible and resistant strains were inoculated.

The peach aphid has been applied according to the spotted mite test method, but only 10 individuals are used per treatment lot.

Biotype Type B, a cigarette but not a pesticide, was grown in host plants (tomatoes) for more than 12 years without insecticide culling. Biotype Q type, an insecticide resistant strain, They were used for experiments in the subculture. Breeding was carried out at a temperature of 26 ° C, a relative humidity of 40-60% and a light condition of 16: 8 hours (note: night). The insecticidal activity test for tobacco rui was carried out in two ways: fumigation activity and contact activity. In the case of the former, the branches cultivated in the plastic pot were arbitrarily selected, and the leaf pods were cut to a suitable size, and the periphery thereof was curled with a cotton swab and fixed on an Eppendorf tube (1.5 ml). They were placed in a 20 ml glass bayer at a rate sufficient to overflow the water and placed vertically in an acrylic cage (6.5, approximately 410 cm ³ ). Approximately 30-35 individuals Adult B, Q biological flour These were introduced into each cage 12-18 hours before the test and induced to settle stably on the back or front of the leaves. The optimum amount of each plant sample was diluted in 50 μl of ethanol, treated uniformly in a filter paper (diameter 4.25 cm), and then volatilized for 90 seconds. The treated wastes were placed on the bottom of each acrylic cage, and the lid was closed to examine the mortality rate after 24 hours. In the latter case, each of the test samples was prepared to a final concentration of 2% in an aqueous solution of 5% ethanol, 0.02% tween 80, residual water and poured into a pump spray container (60 ml). Five times sprayed manually for contact test on the powder settled on the host leaf. The results were examined after 24 hours.

In the insecticidal test for cucumber syrup, the cigarette was tested in the same manner as in the test for adult rui, except that the cucumber was used as a host plant and the number of inoculated individuals was 10 per treatment.

The results of the insecticidal rate (three repeated mean values) of the above experiment are shown in Table 1 below.

Sample name Concentration% Spotted mite Peach aphid The cigarette is Louis Cucumber thrush sensibility Resistance Type B Q type fumigation contact fumigation contact 5 5 10 5mg / room 2 5mg / room 2 5 Anise oil 100 100 88 96 ^* - 100 - 72 Basil oil 98 100 77 88 - 84 - 82 Hyshop oil 100 98 84 74 - 80 - 78 Terpentine oil 100 100 88 84 - 78 - 84 Patolio Day 100 100 96 100 - 100 - 98 Black pepper oil 96 98 89 80 - 92 - 100 Estrain Oil 100 100 100 96 - 92 - 96 Saborio Day 100 99 100 100 - 100 - 96 Armeous oil 94 92 84 96 - 100 - 88 Wintergreen Oil 100 100 92 100 - 100 - 100 Ethyl cinnamate 100 100 92 - 78 - 84 84 Methyl cinnamate 92 88 88 - 72 - 68 80 Cinnamyl acetate 100 100 96 - 88 - 92 84 Methyl salicylate 100 96 84 - 68 - 60 96 Estrago - - 92 - 84 - 72 88 3-pinanone - - 82 - 94 - 90 - Phenethyl 2-methylbutyrate 100 - 88 - 96 - 100 94 Phenethyl acetate 100 100 100 - 100 - 100 98 Phenethyl anthranilate 88 - 96 - 92 - 100 96 Phenethyl benzoate 100 100 100 - 100 - 100 100 Phenethyl butyrate 98 100 88 - 100 - 96 96 Phenethyl cinnamate 100 88 92 - 88 - 96 100 Phenethyl hexanoate 100 100 98 - 92 - 98 92 Phenethyl isobutyrate 100 100 96 - 96 - 100 96 Phenethyl isovalerate 100 92 98 - 92 - 88 92 Phenethyl octanoate 98 90 88 - ^* - - 84 92 Phenethyl salicylate 88 96 96 - 84 - - 88 Phenethyl tiglate 100 100 96 - 76 - 84 92 Alpha, alpha-dimethylphenethyl acetate 19 - - - - - - - Alpha-isobutylphenethyl alcohol 96 - 88 - 92 - 96 84 Alpha-methylphenethyl butyrate 100 100 96 - 100 - 100 96 Phenethyl phenylacetate 100 - 100 - 100 - 100 96

^* -, US experiment.

The above results indicate that an anise oil, a basil oil, a hyssop oil, a terpentine oil, a patcholioil, a black pepper oil, an estradiol oil, a saborioil, an amoiose oil, a wintergreen oil and the like for the spotted mite susceptibility and the pyridivine resistance system It shows a strong resistance force. They also showed strong insecticidal activity against peach aphid and cucumber thrips. In addition, it can be seen that the fumigant shows the insecticidal power against the two species of Louis. It can be concluded that these plant oils are a very effective tool for the management of resistant insects.

Although the insecticidal power of the compounds is somewhat different depending on the test species and the method, it is preferable to use a compound such as ethyl cinnamate, methyl cinnamate, cinnamic acetate, methyl salicylate, estragol, 3-pinanone, But are not limited to, ethyl acetate, butylate, phenethyl acetate, phenethyl anthranilate, phenethyl benzoate, phenethyl butyrate, phenethyl cinnamate, phenethyl hexanoate, phenethyl isobutyrate, Phenate salicylate, phenethyl tiglate, isobutylphenethyl alcohol, methylphenyl ethyl butyrate, and phenetyl phenylacetate exhibit strong insecticidal activity at a concentration of 2-5%. The excellent insecticidal effect of insecticide resistant strains has also proved that they can be used as an excellent agricultural material to control insecticide resistant strains.

[Experimental Example 2] Insecticidal (non) property against hygiene and domestic pest

Among the experimental materials, plant essential oil was dissolved in 100 ul of ethanol, and treated with a filter paper (diameter 5 cm). Then, ethanol was volatilized for 1 minute and put into petri dish (diameter 5.5 cm). On top of that, we emitted the appropriate number of house dust mite species (large leg house dust mite, vertical house dust mite). Compounds were sprayed three times onto prepared mites by preparing 4% ethanol preparation. The Salvia outcome was examined 2 hours later on a dissecting microscope (20-40 times). All experiments were performed in the room with 3 repetitions.

The larvae of the mites, which mediate Tsutsugamushi, were collected and used as outbred host plants. Test specimens were dissolved in 5 ml of ethanol at a concentration of 4%, and a black cloth (diameter 5 cm) was placed in a Petri dish (diameter 5.5 cm) and sprayed twice with 10 randomly picked larvae. Four hours after the treatment, the number of lethal populations was examined under a microscope. The determination of lethal populations was considered to be a bug when three or more of the appendages were moved. All tests were performed in 3 replicates.

The insecticidal activity against the moth flies and mosquito adults (red house mosquito and white mosquito mosquito) was also irradiated to the exposed field (10 cm in diameter, 20 cm in height) with 10 adult mosquitoes (mosquitoes only female) % Ethanol solution was sprayed using a high pressure sprayer (benchtop type sprayer). A cotton swab soaked in 10% aqueous sugar solution was provided and insecticidal activity was investigated after 4 hours.

The test results (3 repetitive mean insecticidal rates,%) are shown in Table 2 below.

Sample concentration Big legs house dust mite Vertical house dust mite Mite Red house mosquito White line mosquito Moth flies 4 mg / paper or 4% 4% 10% Anise oil 100 100 100 80 72 68 Basil oil 100 100 100 ^* - - - Hyshop oil 94 88 - - - - Terpentine oil 98 96 84 72 88 78 Patolio Day 100 100 92 100 100 100 Black pepper oil 88 78 100 100 96 84 Estrain Oil 92 98 - - - 88 Saborio Day 100 100 98 78 84 68 Armeous oil 84 80 - - - - Wintergreen Oil 100 100 94 88 92 88 Ethyl cinnamate 78 72 100 - 84 67 Methyl cinnamate 90 92 100 - 78 88 Cinnamyl acetate 100 100 100 96 92 92 Methyl salicylate 100 100 100 92 84 98 Estrago 100 100 100 94 90 58 3-pinanone 94 100 90 - - 44 Phenethyl 2-methylbutyrate 92 92 100 82 78 88 Phenethyl acetate 100 100 100 96 98 96 Phenethyl anthranilate 93 96 93 - - 68 Phenethyl benzoate 100 100 100 100 96 100 Phenethyl butyrate 100 100 100 92 88 100 Phenethyl cinnamate 100 100 92 88 78 96 Phenethyl hexanoate 100 100 100 100 100 92 Phenethyl isobutyrate 100 100 100 100 100 96 Phenethyl isovalerate 100 98 94 84 80 88 Phenethyl octanoate 97 92 88 - - - Phenethyl salicylate 88 92 100 78 68 64 Phenethyl tiglate 100 96 92 - - 68 Alpha, alpha-dimethylphenethyl acetate 19 4 24 - - 0 Alpha-isobutylphenethyl alcohol 97 100 100 100 96 82 Alpha-methylphenethyl butyrate 100 100 100 100 100 92 Phenethyl phenylacetate 100 96 100 - 92 88 Phenethyl formate 84 86 96 - - 76

^* -, did not proceed

These results suggest that the two kinds of house dust mites that are important for the domestic pest that can cause asthma, atopic dermatitis and rhinitis are anise oil, basil oil, heyshoot oil, Salmon Oil, Arumose Oil, and Wintergreen Oil have proven to show strong resistance. Anise oil, Basil oil, Turpentine oil, Pallolioil, Black pepper oil, Saborioil, Wintergreen oil showed strong antimicrobial activity against the mites that mediate Tsutsugamushi symptoms. In addition, these plant essential oils showed strong insecticidal activity against red house mosquito, white mosquito mosquito and moth flies when sprayed. However, the insecticide intensity was weaker than that of ticks.

Similar insecticidal results were obtained using ethyl cinnamate, methyl cinnamate, cinnamyl acetate, methyl salicylate, estragol, 3-pinanone, phenethyl 2-methyl butyrate, phenethyl acetate, phenethyl anthranilate, Phenethyl benzoate, phenethyl benzoate, phenethyl butyrate, phenethyl cinnamate, phenethyl hexanoate, phenethyl isobutyrate, phenethylisobalylate, phenethyl octanoate, phenethyl salicylate, phenethyl tiglate , Isobutylphenethyl alcohol, methylphenyl ethyl butyrate, and phenethyl phenyl acetate.

These results demonstrate that they can be used as materials that can effectively control arthropods important in domestic and hygienic environments.

[Experimental Example 3] Animal parasites

Chicken ticks were collected from a poultry farm in Pocheon, Gyeonggi-do. Of the test samples, 4 mg was dissolved in ethanol 100, and the mixture was uniformly dispersed with a micropipette (4.25 cm in diameter) and the solvent was evaporated for 3 minutes. The treated petri dish was treated with petri dish (diameter 4.8 cm, height 1.25 cm < / RTI > Each petri dish was pre-treated with 50% deionized water at 0.5 1 cm to maintain adequate relative humidity. Fifteen to thirty adult chick moths with excellent vitality were irradiated on the treated material. After 24 hours, the lid of the petridish was opened under a dissecting microscope (), and the animals were directly stimulated with fine pins, Were regarded as cattle. The compounds were sprayed directly onto the mites three times with a sprayer at a concentration of 10% in ethanol and the results were examined after 3 hours. All tests were performed in 3 replicates.

In order to test for inactivation of ear mite, which is mainly parasitic on a pet cat or dog 's outer ear, mites collected from a pet shop were tested in the same manner as the chick tick test described above. The difference was that the number of mite treatments was 10 individuals and that it was done with 2 repetitions. These differences are due to relatively difficult access to these ticks.

The results (average of three reproductive rates) are shown in Table 3 below.

Name of sample Chicken tick Ear mites Anise oil 100 92 Basil oil 88 84 Hyshop oil 78 66 Terpentine oil 74 72 Patolio Day 100 88 Black pepper oil 78 68 Estrain Oil 100 74 Saborio Day 100 100 Armeous oil 96 84 Wintergreen Oil 92 88 Ethyl cinnamate 64 78 Methyl cinnamate 72 72 Cinnamyl acetate 88 92 Methyl salicylate 96 76 Estrago 68 56 3-pinanone 72 60 Phenethyl 2-methylbutyrate 92 - Phenethyl acetate 100 88 Phenethyl anthranilate 92 84 Phenethyl benzoate 100 96 Phenethyl butyrate 100 78 Phenethyl cinnamate 88 68 Phenethyl hexanoate 96 72 Phenethyl isobutyrate 100 88 Phenethyl isovalerate 92 84 Phenethyl octanoate 84 78 Phenethyl salicylate 80 64 Phenethyl tiglate 92 78 Alpha, alpha-dimethylphenethyl acetate 33 20 Alpha-isobutylphenethyl alcohol 100 88 Alpha-methylphenethyl butyrate 98 92 Phenethyl phenylacetate 100 92

The above results indicate that the chicken mites and ear mites, which are external parasites of animals, can be used as an anemia oil, a basil oil, a hysopo oil, a terpentine oil, a pacolioil, a black pepper oil, an estradiol oil, a saborioil, The 10% spray liquid such as winter oil showed a strong resistance.

Similar anti-aging effects can be achieved with ethyl cinnamate, methyl cinnamate, cinnamic acetate, methyl salicylate, estragol, 3-pinanone, phenethyl 2-methyl butyrate, phenethyl acetate, phenethyl anthranilate, But are not limited to, ethyl acetate, isobutyl acetate, isobutyl acetate, isobutyl acetate, isobutyl acetate, isobutyl acetate, isobutyl acetate, isobutyl acetate, Methylphenyl ethyl butyrate, phenethyl phenyl acetate, and the like.

These results demonstrate that they can be used as protective material for animals that can effectively control external parasites that are parasitic on livestock and pets.

[Experimental Example 4] Synergy of plant essential oils or compounds

In order to investigate the synergistic action of plant essential oils and compounds, three types of terpentine oil, patolioyl and saborioil were selected and 1%: 1%, 1%: 2% , 2%: 2%, etc. were prepared and tested for ascending force. Each arthropod was irradiated on a leaf or leaf slice of a host plant, sprayed with a mixture of the prepared samples alone or in an appropriate ratio, and examined for insecticidal power after 3 hours of treatment.

The mutual synergism between terpentin oil and some insecticidal compounds (3 repetitive average insecticidal rates) is shown in Table 4 below.

Name of sample ratio House dust mites Spotted mite Mite Peach aphid Terpentine oil One% 3 0 0 0 2% 9 0 12 0 Phenethyl benzoate (PB) One% 9 9 3 3 2% 12 15 3 0 Turpentine 1: PB1% 30 12 21 12 Turpentine 1: PB2% 33 9 24 15 Turpentine 2: PB2% 100 100 100 96 Phenethyl butyrate (PBT) One% 0 0 0 3 2% 15 24 9 3 Turpentine 1: PBt 1% 24 9 33 24 Turpentine 1: PBt 2% 42 51 45 33 Turpentine 2: PBt2% 100 100 92 88 Phenethyl cinnamate (PC) One% 12 12 15 0 2% 24 15 9 3 Turpentine 1: PC1% 21 21 15 9 Turpentine 1: PC2% 27 36 33 39 Turpentine 2: PC2% 100 100 84 76 Phenethyl hexanoate (PH) One% 12 3 3 0 2% 15 15 9 3 Turpentine 1: PH1% 21 33 12 12 Turpentine 1: PH2% 33 39 24 15 Turpentine 2: PH2% 100 100 100 100 Phenethyl isobutyrate (PI) One% 6 3 3 0 2% 24 15 9 9 Turpentine 1: PI1% 9 15 21 21 Turpentine 1: PI2% 44 39 33 24 Turpentine 2: PI2% 100 100 100 92 Phenethyl phenylacetate (PP) One% 12 9 9 6 2% 21 24 18 15 Turpentine 1: PP1% 21 30 24 24 Turpentine 1: PP2% 39 33 36 18 Turpentine 2: PP2% 100 100 100 100

      The results of Table 4 above show that the elevation of some compounds such as terpentine oil and phenylethyl groups was not observed. No oils or compounds showed strong insecticidal activity at 1 or 2% treated groups alone. In addition, the mixture ratio of 1: 1 and 1: 2% showed an increase in insecticidal activity compared with that of monotherapy, but a clear increase, that is, an increase in insecticidal power near perfect, was observed in 2: 2% treatment. This indicates that plant essential oils and compounds exert increased insecticidal power through mutual synergistic function, and this increased insecticidal power is unexpected and very important in pest control. Because these phenylethyl-based compounds are widely used for food additives, they have proved their usefulness for cheap agricultural and health use.

The results of examining the mutual synergism between phenolioil and some insecticidal compounds (3 repetitive mean insecticidal rates) are shown in Table 5 below.

Name of sample ratio House dust mites Spotted mite Mite Peach aphid Patolio Day One% 9 15 12 0 2% 9 12 9 6 Phenethyl benzoate (PB) Patrol 1: PB1% 15 18 15 9 Patrizia 1: PB2% 24 21 33 15 Patrizia 2: PB2% 100 100 100 100 Phenethyl butyrate (PBT) Patrizia 1: PBt1% 12 15 15 9 Patolli 1: PBt2% 33 45 33 30 Patrizia 2: PBt2% 100 100 92 92 Phenethyl cinnamate (PC) Patrol 1: PC1% 9 12 12 0 Patrol 1: PC2% 24 33 30 30 Patrizia 2: PC2% 100 100 92 92 Phenethyl hexanoate (PH) Phosphorus 1: PH1% 9 12 15 3 Phosphorus 1: PH2% 33 48 45 33 Patrizia 2: PH2% 100 100 100 100 Phenethyl isobutyrate (PI) Patrizia 1: PI1% 3 15 18 12 Patrizia 1: PI2% 30 42 45 27 Patrizia 2: PI2% 100 100 100 97 Phenethyl phenylacetate (PP) Patrol 1: PP1% 9 9 12 6 Patrol 1: PP2% 33 39 27 27 Patrizia 2: PP2% 100 100 100 92

As shown in Table 4, as shown in Table 4, it was experimented to ascertain the ascending powers of some compounds of Phezoly oil and phenylethyl group. Non-power). In addition, the mixture ratio of 1: 1 and 1: 2% showed an increase in insecticidal activity compared with that of monotherapy, but a clear increase, that is, an increase in insecticidal power near perfect, was observed in 2: 2% treatment. The results were similar to those of Table 4. In order to more closely examine these unexpected results, the same experiment as the results of Table 6 was carried out again.

Namely, the mutual synergism between the days of the saborium and some insecticidal compounds (3 repetitive average insecticidal rates) is shown in Table 6 below.

Name of sample ratio House dust mites Spotted mite Mite Peach aphid Saborio Day One% 0 0 6 0 2% 12 9 6 3 Phenethyl benzoate (PB) Savory 1: PB1% 27 15 27 33 Savory 1: PB2% 33 36 42 45 Savory 2: PB2% 100 100 100 100 Phenethyl butyrate (PBT) Savory 1: PBt1% 24 9 33 24 Savory 1: PBt2% 45 48 42 36 Savory 2: PBt2% 100 100 100 97 Phenethyl cinnamate (PC) Savory 1: PC1% 12 12 0 0 Savory 1: PC2% 27 33 33 24 Savory 2: PC2% 100 100 97 92 Phenethyl hexanoate (PH) Savory 1: PH1% 24 42 33 12 Savory 1: PH2% 33 36 30 15 Savory 2: PH2% 100 100 100 100 Phenethyl isobutyrate (PI) Savory 1: PI1% 12 18 12 21 Savory 1: PI2% 45 42 39 33 Savory 2: PI2% 100 100 100 100 Phenethyl phenylacetate (PP) Savory 1: PP1% 15 30 24 12 Savory 1: PP2% 36 45 45 33 Savory 2: PP2% 100 100 100 100

The results of Table 6 above are also shown in Tables 4 and 5 to examine the ascending powers of some compounds of saponin oil and phenylethyl groups. As a result, oils and compounds in a single treatment of 1% or 2% Non-power). In addition, the mixture ratio of 1: 1 and 1: 2% showed an increase in insecticidal activity compared with that of monotherapy, but a clear increase, that is, an increase in insecticidal power near perfect, was observed in 2: 2% treatment. The results are similar to those of Tables 4 and 5.

As a result of the above Tables 4 to 6, it has been found that an increased synergistic effect can be expected by mixing plant-essential oils having insecticidal power and insecticidal compounds having a specific structure in the compound such as phenylethyl group. Therefore, the present invention finally shows that the compound having the phenylethyl group and the plant essential oil having insecticidal power can be expected to increase the insecticidal power through the synergistic action. I think this is a big application development that can be used in the field of pest control in the future.

Claims (8)

Having insecticidal or acaricidal activity against insects selected from the group consisting of mites, flour, aphids, thrips, house dust mites, mites, moth flies, mosquitoes, chicken mites and ear mites
(A) a plant essential oil selected from the group consisting of anise oil, basil oil, hyssop oil, terpentine oil, petroleum jelly, black pepper oil, estrogen oil,
(B) ethyl cinnamate, methyl cinnamate, cinnamyl acetate, methyl salicylate, estragol, 3-pinanone, phenethyl 2-methylbutyrate, But are not limited to, phenethyl acetate, phenethyl anthranilate, phenethyl benzoate, phenethyl butyrate, phenethyl cinnamate, phenethyl hexanoate, Phenethyl isobutyrate, Phenethyl isovalerate, Phenethyl octanoate, Phenethyl salicylate, Phenethyl tiglate, Phenethyl isobutyrate, ), An isobutylphenethyl alcohol, methylphenethyl butyrate, and phenethyl phenylacetate. The phenylacetyl group is preferably selected from the group consisting of isobutylphenethyl alcohol, methylphenethyl butyrate, and phenethyl phenylacetate. Agricultural chemical composition comprising the water. delete delete delete delete delete delete delete