KR102096720B1 - Low―toxic and eco-friendly composition for controlling Dermanyssus gallinae having Formic Acid and method for manufacturing the same - Google Patents

Abstract

The present invention relates to a low-toxic and eco-friendly composition for controlling Dermanyssus gallinae using formic acid, and a method for preparing the same. The composition includes 3-4 wt% of formic acid in combination with the balance amount of a solvent. The composition may further include 0.1-3 wt% of a surfactant to enhance the efficiency of controlling Dermanyssus gallinae of formic acid and to sterilize salmonella remaining on shells of edible eggs. The composition for controlling Dermanyssus gallinae uses an eco-friendly material, formic acid, and thus is not harmful even when it is applied directly to edible eggs, and causes no problems related with resistance and residual agricultural chemical ingredients in environment. In addition, the composition has an excellent effect of controlling Dermanyssus gallinae and an excellent sterilizing effect against salmonella remaining on shells of edible eggs.

Description

Low toxicity and eco-friendly composition for controlling Dermanyssus gallinae having Formic Acid and method for manufacturing the same}

The present invention relates to a low toxicity eco-friendly chicken mite control composition using formic acid and a method for manufacturing the same, more specifically, it has excellent chicken mite control effect, and can effectively control chicken mite using formic acid, even if it is sprayed directly into the edible egg. Safe, edible relates to a low-toxic, eco-friendly chicken mite control composition that has a sterilizing effect against Salmonella remaining in the egg shell and a method for manufacturing the same.

The chicken mite ( Dermanyssus gallinae De Geer; Acaridae) corresponds to the arachnid mite, and is a nocturnal pest that parasites and vampires chickens, wild birds and rodents with a size of 0.7 to 1.0 mm. Silver is a vampire parasite that can cause economic loss to poultry farms, as it can cause spawning rates or even group death due to stress, such as anemia or itching. The National Institute for Livestock Science, Poultry Research Institute of the Rural Development Administration estimates that 94% of domestic poultry farms have chicken mite infection.

The life cycle from chicken mite to adulthood usually takes about 7 to 10 days, and the stage of vampire in their life cycle is usually the larvae stage and adult stage, about 3 to 5 days after hatching. Chicken mites lay 4 to 8 eggs at a time and spawn on average 5 to 6 times, so if they are not efficiently controlled in time due to the characteristics of short chicken mites, their growth rate can progress exponentially rapidly.

In order to increase the insecticidal efficiency of chicken mites, chemical pesticides that have not been tested for the safety of ingredients that are not intended to control chicken mites are used indiscriminately, and organic synthetic pesticide control agents that negatively suppress pests of crops are also used.

In addition, in principle, chicken mite control agents should be applied to empty chickens without chickens or eggs, but it is not easy to empty tens of thousands of chickens at a time in laying farms, and chemical pesticides are often sprayed with the laying eggs and edible eggs intact. Is known. In the process, chemical pesticide components harmful to the human body may be absorbed into the body of the chicken or left on the surface of the egg. In addition, most chemical pesticides can continue to increase resistance due to resistance problems when applied continuously, and have neurotoxins that block the signaling of nerve cell membranes, raising chickens due to food hygiene, pesticide residues, environmental pollution, and worker toxicity problems. There was a problem that the items that can be used are limited.

Republic of Korea Patent No. 10-1295228 is a pesticide for poultry and poultry disinfection, which includes formic acid and pyridaben and excipients, which are the active ingredients of the insecticidal agent for tick anthelmintic, and has a rapid and continuous killing effect on chicken mites. There are advantages.

Formic acid is a natural acid extracted from plants such as nettle, and its molecular formula is CH ₂ O ₂ , the simplest structure of carboxylic acid.

However, high concentration of formic acid solution can strongly irritate the skin, eyes, mucous membranes, etc. and cause discoloration and surface loss of the edible eggshell, especially when pyridaben is exposed to the human body for a long period of time, it affects the nervous system and causes side effects such as weight loss. This may appear and is known as one of the ingredients that should not be detected in the edible egg.

In fact, in August 2017, the Ministry of Agriculture, Food and Rural Affairs conducted a total inspection of 1,239 laying farms in Korea. As a result, pesticide ingredients that were prohibited or exceeded the standard were detected in approximately 52 general farms and eco-friendly farms, and were judged to be unsuitable. The pesticide components detected were fipronil, bifenthrin, flutenoxuron, ethoxazole, and pyridaben, and all eggs of farmers who were judged to be non-conforming were discontinued from shipment and discarded in full. Studies have shown that some of the components detected in farmhouses are classified as potentially carcinogenic substances or may cause abnormalities in the liver, thyroid, and kidneys when consumed in large quantities. As a result, consumers' anxiety has increased, and there is an urgent need for safe food that can be trusted and eaten.

In addition, one of the components that may be harmful to the human body in poultry is Salmonella spp. , Which causes typhoid disease and causes food poisoning. Salmonella bacteria are known to remain in the eggshell for various reasons or penetrate into the edible egg and cause acute enteritis, typhoid fever, or food poisoning. In August 2018, 2,207 group food poisonings occurred as products contaminated by Salmonella in egg shells were distributed to school meals.

Accordingly, there is a need to develop a chicken mite control composition that is safe even if it is applied directly to the edible egg by supplementing the disadvantages of the chemical pesticide and has a bactericidal effect against Salmonella remaining in the egg shell.

The present invention was invented by the above-mentioned demands, and formic acid which has excellent remedy for chicken mites (wagumo) that inhabit poultry and cause problems such as weight loss, decreased egg production, increased stress, and disease transmission in animals. Provided is a control composition used and a method for manufacturing the same.

At the same time, it is safe to spray directly on the edible egg, and provides a low-toxic, eco-friendly chicken mite control composition using formic acid that has a bactericidal effect against salmonella remaining in the egg shell and a method for manufacturing the same.

The present invention provides a low-toxic eco-friendly chicken mite control composition using formic acid and a method for manufacturing the same, comprising formic acid and a solvent to achieve the above object.

The present inventors have researched on a low-toxic eco-friendly chicken mite control composition that effectively controls chicken mites, but is also effective against Salmonella remaining in the eggshell of the edible egg. The present invention was completed by determining the surfactant and additive concentration range effective for Salmonella remaining in the edible egg shell while simultaneously improving the control efficiency of chicken mite of formic acid.

The chicken mite control composition of the present invention is preferably a control agent for any one or more selected from the group consisting of house dust mite, hair mite, chicken mite and ear mite, and more specifically, it is more preferably a mite control composition, but is not limited thereto. Does not.

The chicken mite control composition provides a low-toxic eco-friendly chicken mite control composition using formic acid, characterized in that 3 to 4% by weight of formic acid is mixed with a residual amount of a solvent.

The solvent may be selected from the group consisting of water, ethanol, methanol, ethyl acetate, acetone, N-methylpyrrolidone, dimethyl sulfoxide, and di methyl formamide.

In addition, 0.1 to 3% by weight of surfactant may be further included in order to improve the efficiency of controlling chicken mite of formic acid and sterilize Salmonella remaining in the edible egg shell.

The surfactant may be selected from one or more of the group consisting of monoisotridecyl phosphate, diisotridecyl phosphate, polyoxyethylene octylphenyl ether, polyoxyethylene tridecyl ether and polyoxyethylene ethylhexyl ether.

Adhesives, dispersants, stabilizers and preservatives may be added to the chicken mite control composition, if necessary.

The low-toxic eco-friendly chicken mite control composition using formic acid according to the present invention has the advantage of being less toxic and safe even when sprayed directly onto an edible egg with low toxicity. In addition, since it uses eco-friendly materials, not organic synthetic pesticides or artificial chemical pesticides, it has the advantage that resistance problems and environmental residues of pesticide components do not occur. In addition, chicken mite control efficiency is also excellent, which reduces the stress of the spawning system, increases the spawning rate, and furthermore, it has antiseptic effect on Salmonella bacteria remaining in the edible egg shell, which can be expected to improve farming competitiveness and restore consumer confidence.

Hereinafter, a low toxicity eco-friendly chicken mite control composition using formic acid according to the present invention and a method for manufacturing the same will be described in detail by the following examples. However, the following examples are only illustrative of the present invention, and the contents of the present invention are not limited by the following examples.

The low toxicity eco-friendly chicken mite control composition using formic acid according to the present invention is composed of formic acid and a solvent, and is preferably made by mixing formic acid 3 to 4% by weight and a residual amount of a solvent.

The formic acid exhibits the disinfecting power of the chicken mite control composition of the present invention, while exhibiting a pesticidal effect on the chicken mite. If the content of formic acid is less than 3% by weight, the insecticidal effect of the chicken mite control composition of the present invention is lowered, and if the content of formic acid is more than 4% by weight, the egg shell of the edible egg shows discoloration and surface dropping phenomenon. .

The solvent may be used to function to disperse the formic acid and surfactant smoothly to be diluted. Preferably, one or two or more may be selected from the group consisting of water, ethanol, methanol, ethyl acetate, acetone, N-methylpyrrolidone, dimethyl sulfoxide and di methyl formamide.

In addition, 0.1 to 3% by weight of surfactant may be further included in order to improve the efficiency of controlling chicken mite of formic acid and sterilize Salmonella remaining in the edible egg shell.

The surfactant is adsorbed to the target microbial cell surface and then destroys the cell membrane to inactivate the microorganism and serves to diffuse the insecticidal component of the chicken mite control composition of the present invention to the treated surface. When the content of the surfactant is less than 0.1% by weight, the surface diffusion effect of the chicken mite control composition of the present invention is lowered, and when the content of the surfactant is more than 3% by weight, the surface diffusion effect is no longer improved. , The relative content of formic acid decreases, and the insecticidal effect of the control composition decreases.

The surfactant has the best control efficiency against chicken mite when mixed with formic acid, and a material having excellent sterilizing effect against Salmonella remaining in the edible egg shell, specifically, monoisotridecylphosphate, diisotridecylphosphate, polyoxy One or two or more may be selected from the group consisting of ethylene octyl phenyl ether, polyoxyethylene tridecyl ether and polyoxyethylene ethyl hexyl ether.

In addition, in order to further enhance the control effect of chicken mite, the chicken mite control composition of the present invention may be used by mixing purslane extract and / or weasel fern extract.

Purslane ( Portulaca oleracea. L.) is a 1-year-old weed that grows mainly on roadsides and gardens. It grows to a height of about 15 ~ 30cm. The extract of purslane is known to have a sterilizing and insecticidal effect, and is mixed with other plant extracts or insecticidal ingredients to be commercialized as several pesticides, but has not yet been commercialized as a chicken mite control composition.

As a method of extracting purslane, 100 g of purslane leaves were ground in a blender, mixed with 10 times the purslane leaves and left to stand at 45 to 55 ° C for 24 hours, and the filtered extract was rotated under reduced pressure at 45 to 55 ° C. After concentration with a concentrator (EYELA N-1000, Japan), the powder can be obtained through a freeze-drying process.

Amorpha fruticosa ( Amorpha fruticosa ) is a deciduous shrub of the Rosaceae legume, also known as a parsley or a weasel. The leaf of a weasel fern contains rutin, and the seed contains amorphine, a rotenoid-based glycoside. Rutin and amorphine in weasel sari are known to have repellent and insecticidal effects against insects and fungi.

As a method of extracting the weasel fern, 100 g of the leaves and seeds of the weasel crushed were crushed in a blender, mixed with 10 times the methanol of the weasel fern and the seeds were left at 45 to 55 ° C. for 24 hours, and the filtered extraction solution was then 45 ~ After concentration at 55 ° C with a rotary vacuum concentrator (EYELA N-1000, Japan), the powder can be obtained through a lyophilization process.

The purslane extract and weasel fern extract may further be added to 4 to 7% by weight to the chicken mite control composition of the present invention.

In addition, an adhesive, a dispersing agent, a stabilizing agent and a preservative may be added to the chicken mite control composition as necessary.

In order to evaluate the chicken mite pesticidal composition of the chicken mite control composition prepared by the following examples, the method for measuring the efficacy test for each composition was published in the 'Efficacy Test Guidelines for Infectious Disease Prevention Pesticides' published by the Food and Drug Administration in 2008. Accordingly, the efficacy test was conducted at the animal hygiene laboratory in northern Gyeonggi Province.

<Example 1> Formic acid efficacy confirmation

In the formic acid efficacy test, formic acid was mixed with 3% by weight, 5% by weight, 7% by weight, and 10% by weight with a residual amount of solvent, and in Example 1, purified water from which impurities were removed was used. The composition prepared in Example 1 was injected into a chicken mite for 7 seconds, and the insecticidal rate of chicken mite was examined after 24 hours. The average insecticidal rate for each concentration was investigated three times, and the average insecticidal rate was calculated by applying the corrected insecticidal rate when dead individuals were seen in the control group.

Corrected insecticide rate = (mortality rate in the experimental group-mortality rate in the control group) / (100-mortality rate in the control group)

Treatment concentration
(weight%) Chicken tick insecticidal rate (%) 1 time Episode 2 3rd time Average 3% 78.2 82.6 86.9 82.6 5% 95.6 95.6 91.3 94.2 7% 100 100 95.6 98.7 10% 100 100 100 100

In Table 1, it was confirmed that the formic acid mixture exhibited an insecticidal effect of chicken mite of 80% or more at all concentrations of 3 to 10% by weight.

<Example 2> Change test when spraying formic acid edible egg

In the formic acid edible egg spray test, formic acid was mixed with 3% by weight, 4% by weight, 5% by weight, and 6% by weight with a residual amount of solvent. In Example 2, purified water from which impurities were removed was used. The composition prepared in Example 2 was sprayed onto the edible egg, and the surface change of the edible egg was examined after 24 hours. It was irradiated three times for each concentration.

Treatment concentration
(weight%) Change of egg shell surface when spraying edible eggs 1 time Episode 2 3rd time 3% clear clear clear 4% clear clear clear 5% Discoloration and surface dropping Discoloration and surface dropping Discoloration and surface dropping 6% Discoloration and surface dropping Discoloration and surface dropping Discoloration and surface dropping

In Table 2, it was confirmed that the formic acid mixture showed discoloration and surface detachment of the edible eggshell at concentrations of 5% by weight and 6% by weight, and it was confirmed that the formic acid mixture of 5% by weight or more was not safe when sprayed directly onto the edible egg.

<Example 3> Preparation of formic acid, solvent and surfactant mixture

The composition was prepared by mixing 3% by weight of the formic acid, 4% by weight of the formic acid, and 3% by weight of the surfactant and the remaining amount of the solvent. In the above Example 3, purified water from which impurities were removed was used, and the surfactant was monoisotridecyl phosphate-diisotridecyl phosphate polymer, polyoxyethylene octylphenyl ether, polyoxyethylene tridecyl ether, and polyoxyethylene ethylhexyl. Ethero was used.

The solvent, formic acid, and surfactant were added to each of the stirrers in turn and mixed, and the stirring time is preferably 15 to 20 minutes of stirring at 50 to 100 rpm, and stirring is terminated when the stirring solution becomes transparent. The stirrer is generally used in the art, and is not limited to the type as long as it can operate from 0 to 2000 rpm based on 1000L.

<Example 4> Insecticidal assay test for chicken mites

The composition prepared in Example 3 was sprayed on a chicken mite for 7 seconds, and the insecticidal rate of chicken mite was examined after 24 hours. The average insecticidal rate for each concentration was repeatedly examined three times, and when the dead individuals were seen in the control group, the average emergence rate was calculated by correcting the calibrated insecticide rate.

Corrected insecticide rate = (mortality rate in the experimental group-mortality rate in the control group) / (100-mortality rate in the control group)

Treatment concentration
(Unit: weight%, residual amount: purified water) Chicken tick insecticidal rate (%) 1 time Episode 2 3rd time Average 3% formic acid
+ 3% monoisotridecyl phosphate
T-diisotridecylphosphate polymer 94.9 97.0 95.6 95.2 4% formic acid
+ 3% monoisotridecylphosphate
-Diisotridecylphosphate polymer 96.2 98.5 95.7 96.2 3% formic acid
+ 3% polyoxyethylene octylphenyl ether 96.1 97.5 95.6 96.1 4% formic acid
+ 3% polyoxyethylene octylphenyl ether 97.8 99.2 99.8 98.8 3% formic acid
+ 3% polyoxyethylene tridecyl ether 94.5 96.7 96.0 95.3 4% formic acid
+ 3% polyoxyethylene tridecyl ether 98.1 96.8 97.5 97.6 3% formic acid
+ 3% polyoxyethylene ethylhexyl ether 97.8 96.4 98.2 97.1 4% formic acid
+ 3% polyoxyethylene ethylhexyl ether 99.7 97.3 98.6 98.5

In Table 3, it was confirmed that the 3% by weight, 4% by weight of formic acid and 3% by weight surfactant mixture composition all exhibited an excellent chicken mite insecticidal effect of 95% or more, and the chicken mite insecticidal efficacy of formic acid by the surfactant. It was confirmed that it was elevated. In particular, the 4% by weight formic acid and 3% by weight polyoxyethylene octyl phenyl ether mixture composition is an average of 98.8%, the 4% by weight formic acid and 3% by weight polyoxyethylene ethylhexyl ether mixture composition is an average of 98.5% excellent chicken mite pesticide It confirmed that it showed an effect.

<Example 5> Salmonella bacteria sterilization test

Salmonella typhimurium was used by pre-sale from the National Veterinary Science and Quarantine Service, and active bacteria were passaged through LB liquid medium 2-3 times in a row. The temperature was incubated at 37 ° C for 22 to 26 hours, and then maintained at 37 ° C until immediately before use.

To the cultured Salmonella (1 × 10 ⁶ cfu / mL), each of the compositions prepared in Example 3 was administered. After plating at 100 µL on LB solid plate medium at 4 hour intervals and incubating at 37 ° C, colonies formed were counted to investigate the survival rate of Salmonella according to the composition concentration and exposure time. The average survival rate for each concentration was examined three times.

Treatment concentration (unit:% by weight, residual amount: purified water) Salmonella survival rate (%) 0h 4h 8h 12h 3% formic acid
+ 3% monoisotridecylphosphate
-Diisotridecylphosphate polymer 100 72.7 64.4 0 4% formic acid
+ 3% monoisotridecylphosphate
-Diisotridecylphosphate polymer 100 56.5 40.2 0 3% formic acid
+ 3% polyoxyethylene octylphenyl ether 100 81.8 47.4 0 4% formic acid
+ 3% polyoxyethylene octylphenyl ether 100 75.3 39.1 0 3% formic acid
+ 3% polyoxyethylene tridecyl ether 100 72.8 68.7 0 4% formic acid
+ 3% polyoxyethylene tridecyl ether 100 65.9 41.6 0 3% formic acid
+ 3% polyoxyethylene ethylhexyl ether 100 89.3 55.5 0 4% formic acid
+ 3% polyoxyethylene ethylhexyl ether 100 71.9 43.3 0

In Table 4, it was confirmed that the Salmonella bactericidal effect of the mixed composition for 3% by weight, 4% by weight of formic acid and 3% by weight of the surfactant exhibits a complete Salmonella bactericidal effect after 12 hours of treatment.

As shown in Tables 1 to 4, the composition in which 4% by weight of formic acid, 3% by weight of surfactant, and the remaining amount of purified water were mixed is safe and does not cause discoloration and surface dropping of the egg shell when sprayed directly onto the edible egg, 95% It was confirmed that the optimal combination of chicken mite control composition exhibiting excellent chicken mite insecticidal effect and complete sterilization effect on Salmonella after 12 hours of treatment.

Although the present invention has been described with reference to exemplary embodiments, those skilled in the art to which the present invention pertains may make various changes without departing from the scope of the present invention. In addition, the invention is not to be limited to the specific embodiments disclosed as the best mode planned for practicing the invention, but it should be construed that the invention includes all embodiments falling within the scope of the appended claims.

Claims (3)

delete In the manufacturing method of a low-toxic eco-friendly chicken mite control composition using formic acid,
The method for preparing the chicken mite control composition is prepared by further mixing purslane extract with the chicken mite control composition prepared by mixing solvents 93 to 96.9 wt%, formic acid 3 to 4 wt% and surfactant 0.1 to 3 wt%,
The method of extracting the purslane extract is ground with a blender and then mixed with methanol corresponding to 10 times the weight of purslane leaves and left at 45 to 55 ° C for 24 hours, followed by filtering the extracted extract solution at 45 to 55 ° C. The method uses the method of concentrating with a rotary vacuum decompressor.
The surfactant is one or two or more mixtures selected from the group consisting of monoisotridecyl phosphate, diisotridecyl phosphate, polyoxyethylene octylphenyl ether, polyoxyethylene tridecyl ether, and polyoxyethylene ethylhexyl ether. Low toxicity environmentally friendly chicken mite control composition using formic acid
According to claim 2,
The method for preparing the chicken mite control composition is prepared by mixing the weasel sage extract instead of the purslane extract mixed with the chicken mite control composition,
The method of extracting the weasel fern extract was triturated with the leaves and seeds of the weasel sari with a blender, mixed with 10 times the weight of the weasel fern leaves and seeds, and left for 24 hours at 45 to 55 ° C., followed by filtering the extracted solution. Method of manufacturing a low-toxic eco-friendly chicken mite control composition using formic acid, characterized by using a method of concentrating with a rotary vacuum decompressor at 45 ~ 55 ℃.