KR102096720B1 - Low―toxic and eco-friendly composition for controlling Dermanyssus gallinae having Formic Acid and method for manufacturing the same - Google Patents
Low―toxic and eco-friendly composition for controlling Dermanyssus gallinae having Formic Acid and method for manufacturing the same Download PDFInfo
- Publication number
- KR102096720B1 KR102096720B1 KR1020180128796A KR20180128796A KR102096720B1 KR 102096720 B1 KR102096720 B1 KR 102096720B1 KR 1020180128796 A KR1020180128796 A KR 1020180128796A KR 20180128796 A KR20180128796 A KR 20180128796A KR 102096720 B1 KR102096720 B1 KR 102096720B1
- Authority
- KR
- South Korea
- Prior art keywords
- formic acid
- control composition
- chicken mite
- composition
- weight
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
본 발명은 개미산을 이용한 저독성의 친환경 닭 진드기 방제조성물 및 이의 제조방법에 관한 것으로서, 더욱 상세하게는 닭 진드기 방제효과가 우수하고, 개미산을 이용해 닭 진드기를 효과적으로 방제할 수 있고, 식용란에 직접 살포되더라도 안전하며, 식용란 난각에 잔류된 살모넬라균에도 살균효과가 있는 저독성의 친환경 닭 진드기 방제조성물 및 이의 제조방법에 관한 것이다.The present invention relates to a low toxicity eco-friendly chicken mite control composition using formic acid and a method for manufacturing the same, more specifically, it has excellent chicken mite control effect, and can effectively control chicken mite using formic acid, even if it is sprayed directly into the edible egg. Safe, edible relates to a low-toxic, eco-friendly chicken mite control composition that has a sterilizing effect against Salmonella remaining in the egg shell and a method for manufacturing the same.
닭 진드기(Dermanyssus gallinae De Geer; Acaridae, 와구모)는 거미강 진드기목에 해당하며, 0.7~1.0mm의 크기로 닭, 야생조류를 비롯한 설치류에 기생하여 흡혈하는 야행성 해충으로, 닭 진드기에 물린 닭은 빈혈이나 가려움 등 스트레스로 인해 산란률이 떨어지거나 심지어는 집단 폐사를 유발할 수 있어, 양계장에 경제적 손실을 크게 일으키는 흡혈성 기생충이다. 농촌진흥청 국립축산과학원 가금연구소는 국내 양계농가의 94%가 닭진드기 감염문제를 가지고 있는 것으로 보고 있다.The chicken mite ( Dermanyssus gallinae De Geer; Acaridae) corresponds to the arachnid mite, and is a nocturnal pest that parasites and vampires chickens, wild birds and rodents with a size of 0.7 to 1.0 mm. Silver is a vampire parasite that can cause economic loss to poultry farms, as it can cause spawning rates or even group death due to stress, such as anemia or itching. The National Institute for Livestock Science, Poultry Research Institute of the Rural Development Administration estimates that 94% of domestic poultry farms have chicken mite infection.
닭 진드기가 알에서 성충이 되기까지의 1 생활사 주기는 보통 7~10일정도 소요되며, 이들의 생활사 중 흡혈을 하는 단계는 대게 부화한 후 3~5일 정도 후인 약충단계와 성충 단계이다. 닭 진드기는 한번에 4~8개의 알을 낳고 평균 5~6번 정도를 산란하므로, 생활사가 짧은 닭 진드기의 특성상 제 때에 효율적으로 방제하지 않으면 증식속도가 따라갈 수 없을 만큼 빠르게 기하급수적으로 진행될 수 있다.The life cycle from chicken mite to adulthood usually takes about 7 to 10 days, and the stage of vampire in their life cycle is usually the larvae stage and adult stage, about 3 to 5 days after hatching. Chicken mites lay 4 to 8 eggs at a time and spawn on average 5 to 6 times, so if they are not efficiently controlled in time due to the characteristics of short chicken mites, their growth rate can progress exponentially rapidly.
양계농가에서는 닭 진드기의 살충 효율을 높이기 위해서 닭 진드기 방제용도가 아닌 성분의 안전성이 검증되지 않은 화학살충제들이 무분별하게 사용되고 있으며, 음성적으로 농작물의 해충을 억제하는 유기합성농약 방제제도 사용되고 있다.In order to increase the insecticidal efficiency of chicken mites, chemical pesticides that have not been tested for the safety of ingredients that are not intended to control chicken mites are used indiscriminately, and organic synthetic pesticide control agents that negatively suppress pests of crops are also used.
또한, 원칙적으로 닭이나 계란이 없는 빈 계사에 닭 진드기 방제제를 살포해야 하지만, 산란계 농가에서는 몇 만 마리의 닭을 일거에 비우기 쉽지 않아, 산란계와 식용란을 그대로 두고 화학살충제를 살포하는 경우가 많다고 알려져 있다. 그 과정에서 인체에 유해한 화학살충제 성분이 닭의 체내로 흡수되거나 계란 표면에 노출되면서 잔류하기도 한다. 또한 대부분의 화학살충제는 지속적으로 적용 시 내성문제로 인해 저항성이 계속 커질 수 있고, 신경세포막의 신호전달을 차단하는 신경독을 가지고 있어, 식품위생, 농약잔류, 환경오염, 작업자의 독성 문제로 닭 사육 중에 사용할 수 있는 품목은 제한적이라는 문제점이 있었다.In addition, in principle, chicken mite control agents should be applied to empty chickens without chickens or eggs, but it is not easy to empty tens of thousands of chickens at a time in laying farms, and chemical pesticides are often sprayed with the laying eggs and edible eggs intact. Is known. In the process, chemical pesticide components harmful to the human body may be absorbed into the body of the chicken or left on the surface of the egg. In addition, most chemical pesticides can continue to increase resistance due to resistance problems when applied continuously, and have neurotoxins that block the signaling of nerve cell membranes, raising chickens due to food hygiene, pesticide residues, environmental pollution, and worker toxicity problems. There was a problem that the items that can be used are limited.
대한민국 등록특허 10-1295228은 개미산(Formic acid)과 진드기 구충용 살충제의 활성성분인 피리다벤(Pyridaben) 및 부형제를 포함하는 축사 및 양계 소독용 살충제로서, 닭 진드기에 신속하고 지속적인 살비효과를 가진다는 장점이 있다.Republic of Korea Patent No. 10-1295228 is a pesticide for poultry and poultry disinfection, which includes formic acid and pyridaben and excipients, which are the active ingredients of the insecticidal agent for tick anthelmintic, and has a rapid and continuous killing effect on chicken mites. There are advantages.
개미산은 천연적으로 쐐기풀 등의 식물에서 추출한 천연산으로서, 분자식은 CH2O2의 가장 간단한 구조의 카르복실산으로, 개미의 독샘에서 발견됐다는 데서 개미산으로 불린다.Formic acid is a natural acid extracted from plants such as nettle, and its molecular formula is CH 2 O 2 , the simplest structure of carboxylic acid.
하지만 고농도의 개미산 용액은 피부와 눈, 점막 등을 강하게 자극하고, 식용란 난각의 탈색 및 표면탈락을 일으킬 수 있으며, 특히 피리다벤은 인체에 장기간 노출될 경우 신경계의 영향을 주어 체중 감소 등의 부작용 증상이 나타날 수 있어, 식용란에서 검출되어서는 안 되는 성분 중 하나로 알려져 있다.However, high concentration of formic acid solution can strongly irritate the skin, eyes, mucous membranes, etc. and cause discoloration and surface loss of the edible eggshell, especially when pyridaben is exposed to the human body for a long period of time, it affects the nervous system and causes side effects such as weight loss. This may appear and is known as one of the ingredients that should not be detected in the edible egg.
실제로 지난 2017년 8월에 농림축산식품부가 국내 1,239개 산란계 농장을 전수검사한 결과, 약 52개의 일반농장과 친환경 농장에서 사용이 금지되거나 기준치를 넘어선 살충제 성분이 검출되어 부적합 판정을 받았다. 검출된 살충제 성분으로는 피프로닐, 비펜트린, 플루테녹수론, 에톡사졸 및 피리다벤 등이 있었으며, 부적합 판정을 받은 농가의 모든 계란이 출하 중지, 전량 폐기되었다. 농가에서 검출된 일부 성분들은 발암 가능성이 있는 물질로 분류되거나 다량 섭취 시 간, 갑상선, 신장 등에 이상을 일으킬 수 있다는 연구결과가 있었다. 이에 소비자들의 불안은 팽배해졌고, 믿고 먹을 수 있는 안전한 먹거리가 절실하게 요구되고 있는 실정이다.In fact, in August 2017, the Ministry of Agriculture, Food and Rural Affairs conducted a total inspection of 1,239 laying farms in Korea. As a result, pesticide ingredients that were prohibited or exceeded the standard were detected in approximately 52 general farms and eco-friendly farms, and were judged to be unsuitable. The pesticide components detected were fipronil, bifenthrin, flutenoxuron, ethoxazole, and pyridaben, and all eggs of farmers who were judged to be non-conforming were discontinued from shipment and discarded in full. Studies have shown that some of the components detected in farmhouses are classified as potentially carcinogenic substances or may cause abnormalities in the liver, thyroid, and kidneys when consumed in large quantities. As a result, consumers' anxiety has increased, and there is an urgent need for safe food that can be trusted and eaten.
또한, 이외에도 양계에서 인체에 유해할 수 있는 성분 중 하나는 티푸스성 질환을 일으키고 식중독의 원인이 되기도 하는 살모넬라균(Salmonella spp.)이다. 살모넬라균은 다양한 원인으로 난각에 잔류되거나 식용란 내부로 침투되며 급성장염, 장티푸스 혹은 식중독 등을 일으키는 것으로 알려져 있다. 최근 2018년 8월, 계란껍질에 묻어있던 살모넬라균에 의해 오염된 제품이 학교 급식소로 유통되어 2,207명의 집단 식중독이 발생되었다.In addition, one of the components that may be harmful to the human body in poultry is Salmonella spp. , Which causes typhoid disease and causes food poisoning. Salmonella bacteria are known to remain in the eggshell for various reasons or penetrate into the edible egg and cause acute enteritis, typhoid fever, or food poisoning. In August 2018, 2,207 group food poisonings occurred as products contaminated by Salmonella in egg shells were distributed to school meals.
이에 따라 화학살충제의 단점을 보완하여 식용란에 직접 살포하더라도 안전하며, 식용란 난각에 잔류된 살모넬라균에도 살균효과가 있는 닭 진드기 방제조성물의 개발이 요구되고 있다.Accordingly, there is a need to develop a chicken mite control composition that is safe even if it is applied directly to the edible egg by supplementing the disadvantages of the chemical pesticide and has a bactericidal effect against Salmonella remaining in the egg shell.
본 발명은 상기와 같은 요구에 의해 발명된 것으로, 양계에 서식하며 동물의 체중감소, 산란율 저하, 스트레스 증대, 질병 전파와 같은 문제를 야기시키는 닭 진드기(와구모)에 대한 구제 효율이 우수한 개미산을 이용한 방제조성물 및 이의 제조방법을 제공한다.The present invention was invented by the above-mentioned demands, and formic acid which has excellent remedy for chicken mites (wagumo) that inhabit poultry and cause problems such as weight loss, decreased egg production, increased stress, and disease transmission in animals. Provided is a control composition used and a method for manufacturing the same.
동시에, 식용란에 직접 살포하더라도 안전하고, 식용란 난각에 잔류된 살모넬라균에도 살균효과가 있는 개미산을 이용한 저독성의 친환경 닭 진드기 방제조성물 및 이의 제조방법을 제공한다.At the same time, it is safe to spray directly on the edible egg, and provides a low-toxic, eco-friendly chicken mite control composition using formic acid that has a bactericidal effect against salmonella remaining in the egg shell and a method for manufacturing the same.
본 발명은 상기와 같은 목적을 달성하기 위하여 개미산 및 용매로 이루어지는 것을 특징으로 하는 개미산을 이용한 저독성의 친환경 닭 진드기 방제조성물 및 이의 제조방법을 제공한다.The present invention provides a low-toxic eco-friendly chicken mite control composition using formic acid and a method for manufacturing the same, comprising formic acid and a solvent to achieve the above object.
본 발명자들은 닭 진드기를 효과적으로 방제하면서도 식용란 난각에 잔류된 살모넬라균에도 효과가 있는 저독성의 친환경 닭 진드기 방제조성물에 대하여 연구한 결과, 닭 진드기 방제 효과를 나타내며 식용란에 직접 살포하더라도 안전한 개미산의 사용 농도범위를 알아내었고, 개미산의 닭 진드기 방제 효율을 우수하게 증진시키면서 동시에 식용란 난각에 잔류된 살모넬라균에도 효과가 있는 계면활성제와 첨가 농도범위를 정하여 본 발명을 완성하였다.The present inventors have researched on a low-toxic eco-friendly chicken mite control composition that effectively controls chicken mites, but is also effective against Salmonella remaining in the eggshell of the edible egg. The present invention was completed by determining the surfactant and additive concentration range effective for Salmonella remaining in the edible egg shell while simultaneously improving the control efficiency of chicken mite of formic acid.
본 발명의 닭 진드기 방제조성물은 집먼지진드기, 털진드기, 닭진드기 및 귀진드기로 이루어진 군으로부터 선택되는 어느 하나 이상에 대한 방제제인 것이 바람직하며, 구체적으로 닭 진드기 방제조성물인 것이 보다 바람직하나 이에 한정되지 않는다.The chicken mite control composition of the present invention is preferably a control agent for any one or more selected from the group consisting of house dust mite, hair mite, chicken mite and ear mite, and more specifically, it is more preferably a mite control composition, but is not limited thereto. Does not.
상기 닭 진드기 방제조성물은 개미산 3 내지 4 중량%와 잔량의 용매가 혼합되어 이루어지는 것을 특징으로 하는 개미산을 이용한 저독성의 친환경 닭 진드기 방제조성물을 제공한다.The chicken mite control composition provides a low-toxic eco-friendly chicken mite control composition using formic acid, characterized in that 3 to 4% by weight of formic acid is mixed with a residual amount of a solvent.
상기 용매는 물, 에탄올, 메탄올, 에틸아세테이트, 아세톤, N-메칠피롤리돈, 디메칠설폭사이드 및 디메칠포름아마이드로 구성된 군으로부터 하나 또는 둘 이상 선택될 수 있다.The solvent may be selected from the group consisting of water, ethanol, methanol, ethyl acetate, acetone, N-methylpyrrolidone, dimethyl sulfoxide, and di methyl formamide.
또한, 개미산의 닭 진드기 방제 효율을 증진시키고 식용란 난각에 잔류된 살모넬라균을 살균하기 위해 계면활성제 0.1 내지 3 중량%가 더 포함될 수 있다.In addition, 0.1 to 3% by weight of surfactant may be further included in order to improve the efficiency of controlling chicken mite of formic acid and sterilize Salmonella remaining in the edible egg shell.
상기 계면할성제는 모노이소트리데실포스페이트, 디이소트리데실포스페이트, 폴리옥시에틸렌옥틸페닐에테르, 폴리옥시에틸렌트리데실에테르 및 폴리옥시에틸렌에틸헥실에테르로 구성된 군으로부터 하나 또는 둘 이상 선택될 수 있다.The surfactant may be selected from one or more of the group consisting of monoisotridecyl phosphate, diisotridecyl phosphate, polyoxyethylene octylphenyl ether, polyoxyethylene tridecyl ether and polyoxyethylene ethylhexyl ether.
상기 닭 진드기 방제조성물에는 필요에 따라서 접착제, 분산제, 안정화제 및 보존제 등이 첨가될 수 있다.Adhesives, dispersants, stabilizers and preservatives may be added to the chicken mite control composition, if necessary.
본 발명에 따른 개미산을 이용한 저독성의 친환경 닭 진드기 방제조성물은 저독성으로 식용란에 직접 살포되더라도 안전하여 약해가 적은 장점을 가진다. 뿐만 아니라, 유기합성 농약 또는 인공화학 살충제가 아닌 친환경적인 소재를 사용하기 때문에 내성문제, 농약 성분의 환경 잔류문제 등이 발생하지 않는 장점도 가진다. 또한, 닭 진드기 구제 효율도 우수하여 산란계의 스트레스를 감소시키고 산란율을 증가시키며, 나아가 식용란 난각에 잔류된 살모넬라균에도 살균효과가 있어, 농가 경쟁력 향상 및 소비자 신뢰회복의 효과를 기대할 수 있다.The low-toxic eco-friendly chicken mite control composition using formic acid according to the present invention has the advantage of being less toxic and safe even when sprayed directly onto an edible egg with low toxicity. In addition, since it uses eco-friendly materials, not organic synthetic pesticides or artificial chemical pesticides, it has the advantage that resistance problems and environmental residues of pesticide components do not occur. In addition, chicken mite control efficiency is also excellent, which reduces the stress of the spawning system, increases the spawning rate, and furthermore, it has antiseptic effect on Salmonella bacteria remaining in the edible egg shell, which can be expected to improve farming competitiveness and restore consumer confidence.
이하, 본 발명에 따른 개미산을 이용한 저독성의 친환경 닭 진드기 방제조성물 및 이의 제조방법을 하기의 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 의해 한정되는 것은 아니다.실시예Hereinafter, a low toxicity eco-friendly chicken mite control composition using formic acid according to the present invention and a method for manufacturing the same will be described in detail by the following examples. However, the following examples are only illustrative of the present invention, and the contents of the present invention are not limited by the following examples.
본 발명에 따른 개미산(Formic acid)을 이용한 저독성의 친환경 닭 진드기 방제조성물은 개미산 및 용매로 이루어지며, 개미산 3 내지 4 중량%와 잔량의 용매를 혼합하여 이루어지는 것이 바람직하다.The low toxicity eco-friendly chicken mite control composition using formic acid according to the present invention is composed of formic acid and a solvent, and is preferably made by mixing formic acid 3 to 4% by weight and a residual amount of a solvent.
상기 개미산은 본 발명의 닭 진드기 방제조성물의 소독력을 나타내는 동시에, 닭 진드기에 살충효과를 나타낸다. 상기 개미산의 함량이 3 중량% 미만으로 함유되면, 본 발명의 닭 진드기 방제조성물의 살충효과가 저하되며, 상기 개미산의 함량이 4 중량% 초과이면, 식용란의 난각이 탈색 및 표면탈락 현상을 보이게 된다.The formic acid exhibits the disinfecting power of the chicken mite control composition of the present invention, while exhibiting a pesticidal effect on the chicken mite. If the content of formic acid is less than 3% by weight, the insecticidal effect of the chicken mite control composition of the present invention is lowered, and if the content of formic acid is more than 4% by weight, the egg shell of the edible egg shows discoloration and surface dropping phenomenon. .
상기 용매는 개미산과 계면활성제를 원활히 분산시켜 희석되도록 하는 기능을 하는 것을 사용할 수 있다. 바람직하게는 물, 에탄올, 메탄올, 에틸아세테이트, 아세톤, N-메칠피롤리돈, 디메칠설폭사이드 및 디메칠포름아마이드로 구성된 군으로부터 하나 또는 둘 이상 선택될 수 있다.The solvent may be used to function to disperse the formic acid and surfactant smoothly to be diluted. Preferably, one or two or more may be selected from the group consisting of water, ethanol, methanol, ethyl acetate, acetone, N-methylpyrrolidone, dimethyl sulfoxide and di methyl formamide.
또한, 개미산의 닭 진드기 방제 효율을 증진시키고, 식용란 난각에 잔류된 살모넬라균을 살균하기 위해, 계면활성제 0.1 내지 3 중량%가 더 포함될 수 있다.In addition, 0.1 to 3% by weight of surfactant may be further included in order to improve the efficiency of controlling chicken mite of formic acid and sterilize Salmonella remaining in the edible egg shell.
상기 계면활성제는 표적 미생물 세포 표면에 흡착된 뒤 세포막을 파괴하여 미생물을 불활성화시키고, 본 발명의 닭 진드기 방제조성물의 살충성분을 처리표면에 확산시키는 역할을 한다. 상기 계면활성제의 함량이 0.1 중량% 미만으로 함유되면, 본 발명의 닭 진드기 방제조성물의 표면 확산효과가 저하되며, 상기 계면황성제의 함량이 3 중량% 초과이면, 표면 확산효과는 더 이상 향상되지 않고, 개미산의 상대적인 함량이 줄어들어 방제조성물의 살충효과가 저하된다.The surfactant is adsorbed to the target microbial cell surface and then destroys the cell membrane to inactivate the microorganism and serves to diffuse the insecticidal component of the chicken mite control composition of the present invention to the treated surface. When the content of the surfactant is less than 0.1% by weight, the surface diffusion effect of the chicken mite control composition of the present invention is lowered, and when the content of the surfactant is more than 3% by weight, the surface diffusion effect is no longer improved. , The relative content of formic acid decreases, and the insecticidal effect of the control composition decreases.
상기 계면활성제는 개미산과 혼합 시 닭 진드기 방제효율이 가장 뛰어나고, 식용란 난각에 잔류된 살모넬라균에도 살균효과가 우수한 물질을 사용하였으며, 구체적으로는 모노이소트리데실포스페이트, 디이소트리데실포스페이트, 폴리옥시에틸렌옥틸페닐에테르, 폴리옥시에틸렌트리데실에테르 및 폴리옥시에틸렌에틸헥실에테르로 구성된 군으로부터 하나 또는 둘 이상 선택될 수 있다.The surfactant has the best control efficiency against chicken mite when mixed with formic acid, and a material having excellent sterilizing effect against Salmonella remaining in the edible egg shell, specifically, monoisotridecylphosphate, diisotridecylphosphate, polyoxy One or two or more may be selected from the group consisting of ethylene octyl phenyl ether, polyoxyethylene tridecyl ether and polyoxyethylene ethyl hexyl ether.
또한, 닭 진드기 방제효과를 더욱 더 증진시키기 위해서는, 본 발명의 닭 진드기 방제조성물에 쇠비름 추출물 및/또는 족제비싸리 추출물을 혼합하여 사용할 수 있다.In addition, in order to further enhance the control effect of chicken mite, the chicken mite control composition of the present invention may be used by mixing purslane extract and / or weasel fern extract.
쇠비름(Portulaca oleracea. L.)은 1년생 잡초로서 주로 길가, 텃밭 등에서 자생하며, 약 15~30cm 높이로 자라며, 줄기는 갈적색으로 땅 위로 비스듬히 퍼져서 생장한다. 쇠비름의 추출물은 살균, 살충효과가 있는 것으로 알려져 있으며, 다른 식물추출물이나 살충성분과 함께 혼합되어 몇몇의 농약으로 상업화되어 있으나, 닭 진드기 방제조성물로는 아직 상용화되지 않았다.Purslane ( Portulaca oleracea. L.) is a 1-year-old weed that grows mainly on roadsides and gardens. It grows to a height of about 15 ~ 30cm. The extract of purslane is known to have a sterilizing and insecticidal effect, and is mixed with other plant extracts or insecticidal ingredients to be commercialized as several pesticides, but has not yet been commercialized as a chicken mite control composition.
쇠비름을 추출하는 방법으로는 쇠비름의 잎 100g을 믹서기에서 마쇄하고, 쇠비름잎 10배의 메탄올을 혼합하여 45~55℃에서 24시간 방치한 후에, 여과시킨 추출용액을 45~55℃에서 회전진공감압농축기(EYELA N-1000, 일본)로 농축한 후, 동결건조과정을 거쳐 파우더를 얻을 수 있다.As a method of extracting purslane, 100 g of purslane leaves were ground in a blender, mixed with 10 times the purslane leaves and left to stand at 45 to 55 ° C for 24 hours, and the filtered extract was rotated under reduced pressure at 45 to 55 ° C. After concentration with a concentrator (EYELA N-1000, Japan), the powder can be obtained through a freeze-drying process.
족제비싸리(Amorpha fruticosa)는 장미목 콩과의 낙엽관목으로, 쪽싸리 또는 왜싸리라고도 하며, 높이는 3m 내외이고, 잎은 싸리나무와 같고 씨앗은 백태콩깍지와 비슷하다. 족제비싸리의 잎에는 루틴이 포함되어 있으며, 씨앗에는 로테노이드계 배당체인 아모르핀 성분이 포함되어 있다. 족제비싸리에 들어있는 루틴과 아모르핀은 충과 균에 대해 기피, 살충효과가 있는 것으로 알려져 있다. Amorpha fruticosa ( Amorpha fruticosa ) is a deciduous shrub of the Rosaceae legume, also known as a parsley or a weasel. The leaf of a weasel fern contains rutin, and the seed contains amorphine, a rotenoid-based glycoside. Rutin and amorphine in weasel sari are known to have repellent and insecticidal effects against insects and fungi.
족제비싸리를 추출하는 방법으로는 족제비싸리의 잎과 종자 100g을 믹서기에서 마쇄하고, 족제비싸리 잎과 종자 10배의 메탄올를 혼합하여 45~55℃에서 24시간 방치한 후에, 여과시킨 추출용액을 45~55℃에서 회전진공감압농축기(EYELA N-1000, 일본)로 농축한 후, 동결건조과정을 거쳐 파우더를 얻을 수 있다.As a method of extracting the weasel fern, 100 g of the leaves and seeds of the weasel crushed were crushed in a blender, mixed with 10 times the methanol of the weasel fern and the seeds were left at 45 to 55 ° C. for 24 hours, and the filtered extraction solution was then 45 ~ After concentration at 55 ° C with a rotary vacuum concentrator (EYELA N-1000, Japan), the powder can be obtained through a lyophilization process.
상기 쇠비름 추출물과 족제비싸리 추출물은 본 발명의 닭 진드기 방제조성물에 각각 4 내지 7 중량%가 더 첨가될 수 있다.The purslane extract and weasel fern extract may further be added to 4 to 7% by weight to the chicken mite control composition of the present invention.
또한, 상기 닭 진드기 방제조성물에는 필요에 따라서 접착제, 분산제, 안정화제 및 보존제 등이 첨가될 수 있다.In addition, an adhesive, a dispersing agent, a stabilizing agent and a preservative may be added to the chicken mite control composition as necessary.
하기 실시예에 의해 제조된 닭 진드기 방제조성물의 닭 진드기 살충력을 평가하기 위하여 각각의 조성물에 대한 효력시험 측정방법은 2008년 식품의약품안전청이 고시한‘전염병예방용 살충제 등의 효력시험 가이드라인’에 따라 경기도북부 동물위생시험소에서 효력시험을 실시하였다.In order to evaluate the chicken mite pesticidal composition of the chicken mite control composition prepared by the following examples, the method for measuring the efficacy test for each composition was published in the 'Efficacy Test Guidelines for Infectious Disease Prevention Pesticides' published by the Food and Drug Administration in 2008. Accordingly, the efficacy test was conducted at the animal hygiene laboratory in northern Gyeonggi Province.
<실시예 1> 개미산의 효능확인<Example 1> Formic acid efficacy confirmation
개미산의 효능확인 시험은 개미산 3 중량%, 5 중량%, 7 중량% 및 10 중량%와 잔량의 용매를 혼합하였으며, 상기 실시예 1에서 용매는 불순물을 제거한 정제수가 사용되었다. 상기 실시예 1에서 제조된 조성물을 닭 진드기에 7초간 분사하고 24시간 후 닭 진드기 살충율을 조사하였다. 각 농도마다 평균 살충율을 3회 반복하여 조사하였으며, 평균 살충율은 대조군에서 죽은 개체가 보일 경우 보정살충율을 적용해 계산하였다.In the formic acid efficacy test, formic acid was mixed with 3% by weight, 5% by weight, 7% by weight, and 10% by weight with a residual amount of solvent, and in Example 1, purified water from which impurities were removed was used. The composition prepared in Example 1 was injected into a chicken mite for 7 seconds, and the insecticidal rate of chicken mite was examined after 24 hours. The average insecticidal rate for each concentration was investigated three times, and the average insecticidal rate was calculated by applying the corrected insecticidal rate when dead individuals were seen in the control group.
보정 살충율 = (실험군의 치사율-대조군의 치사율)/(100-대조군의 치사율)Corrected insecticide rate = (mortality rate in the experimental group-mortality rate in the control group) / (100-mortality rate in the control group)
(중량%)Treatment concentration
(weight%)
상기 표 1에서 개미산 혼합물은 3 내지 10 중량%의 모든 농도에서 80%이상의 닭 진드기 살충효과를 나타내는 것을 확인하였다.In Table 1, it was confirmed that the formic acid mixture exhibited an insecticidal effect of chicken mite of 80% or more at all concentrations of 3 to 10% by weight.
<실시예 2> 개미산 식용란에 분무시 변화 시험<Example 2> Change test when spraying formic acid edible egg
개미산 식용란에 분무시 변화 시험은 개미산 3 중량%, 4 중량%, 5 중량% 및 6 중량%와 잔량의 용매를 혼합하였으며, 상기 실시예 2에서 용매는 불순물을 제거한 정제수가 사용되었다. 상기 실시예 2에서 제조된 조성물을 식용란에 분사하고, 24시간 후 식용란의 표면 변화를 조사하였다. 각 농도마다 3회 반복하여 조사하였다.In the formic acid edible egg spray test, formic acid was mixed with 3% by weight, 4% by weight, 5% by weight, and 6% by weight with a residual amount of solvent. In Example 2, purified water from which impurities were removed was used. The composition prepared in Example 2 was sprayed onto the edible egg, and the surface change of the edible egg was examined after 24 hours. It was irradiated three times for each concentration.
(중량%)Treatment concentration
(weight%)
상기 표 2에서 개미산 혼합물은 5 중량%와 6 중량%의 농도에서 식용란 난각의 탈색 및 표면탈락 현상이 보이는 것을 확인하였으며, 5 중량% 이상의 개미산 혼합물은 식용란에 직접 살포시 안전하지 못하다는 것을 확인하였다.In Table 2, it was confirmed that the formic acid mixture showed discoloration and surface detachment of the edible eggshell at concentrations of 5% by weight and 6% by weight, and it was confirmed that the formic acid mixture of 5% by weight or more was not safe when sprayed directly onto the edible egg.
<실시예 3> 개미산, 용매 및 계면활성제 혼합물의 제조<Example 3> Preparation of formic acid, solvent and surfactant mixture
상기 개미산 3 중량%, 4 중량%와 상기 계면활성제 3 중량% 및 잔량의 용매를 혼합하여 조성물을 제조하였다. 상기 실시예 3에서 용매는 불순물을 제거한 정제수가 사용되었고, 계면활성제는 모노이소트리데실포스페이트-디이소트리데실포스페이트 중합체, 폴리옥시에틸렌옥틸페닐에테르, 폴리옥시에틸렌트리데실에테르 및 폴리옥시에틸렌에틸헥실에테르로가 사용되었다.The composition was prepared by mixing 3% by weight of the formic acid, 4% by weight of the formic acid, and 3% by weight of the surfactant and the remaining amount of the solvent. In the above Example 3, purified water from which impurities were removed was used, and the surfactant was monoisotridecyl phosphate-diisotridecyl phosphate polymer, polyoxyethylene octylphenyl ether, polyoxyethylene tridecyl ether, and polyoxyethylene ethylhexyl. Ethero was used.
상기 용매, 개미산 및 계면활성제는 교반기에 각각 차례대로 투입되어 혼합되었고, 교반시간은 50 내지 100rpm에서 15분 내지 20분 교반을 실시하는 것이 바람직하며, 교반액이 투명해지면 교반을 종료한다. 상기 교반기는 당업계에서 통상적으로 사용하는 것으로 1000L 기준 0 내지 2000rpm을 운용할 수 있는 것이면 그 종류에 한정을 두지 않는다.The solvent, formic acid, and surfactant were added to each of the stirrers in turn and mixed, and the stirring time is preferably 15 to 20 minutes of stirring at 50 to 100 rpm, and stirring is terminated when the stirring solution becomes transparent. The stirrer is generally used in the art, and is not limited to the type as long as it can operate from 0 to 2000 rpm based on 1000L.
<실시예 4> 닭 진드기에 대한 살충력 검정 시험<Example 4> Insecticidal assay test for chicken mites
상기 실시예 3에서 제조된 조성물을 닭 진드기에 7초간 분사하고, 24시간 후 닭 진드기 살충률을 조사하였다. 각 농도마다 평균 살충율을 3회 반복하여 조사하였으며, 대조군에서 죽은 개체가 보일 경우는 보정살충율로 보정하여 평균 상출율을 계산하였다.The composition prepared in Example 3 was sprayed on a chicken mite for 7 seconds, and the insecticidal rate of chicken mite was examined after 24 hours. The average insecticidal rate for each concentration was repeatedly examined three times, and when the dead individuals were seen in the control group, the average emergence rate was calculated by correcting the calibrated insecticide rate.
보정 살충율 = (실험군의 치사율-대조군의 치사율)/(100-대조군의 치사율)Corrected insecticide rate = (mortality rate in the experimental group-mortality rate in the control group) / (100-mortality rate in the control group)
(단위:중량%, 잔량:정제수)Treatment concentration
(Unit: weight%, residual amount: purified water)
+ 3% 모노이소트리데실포스페이
트-디이소트리데실포스페이트 중합체3% formic acid
+ 3% monoisotridecyl phosphate
T-diisotridecylphosphate polymer
+ 3% 모노이소트리데실포스페이트
-디이소트리데실포스페이트 중합체4% formic acid
+ 3% monoisotridecylphosphate
-Diisotridecylphosphate polymer
+ 3% 폴리옥시에틸렌옥틸페닐에테르3% formic acid
+ 3% polyoxyethylene octylphenyl ether
+ 3% 폴리옥시에틸렌옥틸페닐에테르4% formic acid
+ 3% polyoxyethylene octylphenyl ether
+ 3% 폴리옥시에틸렌트리데실에테르3% formic acid
+ 3% polyoxyethylene tridecyl ether
+ 3% 폴리옥시에틸렌트리데실에테르4% formic acid
+ 3% polyoxyethylene tridecyl ether
+ 3% 폴리옥시에틸렌에틸헥실에테르3% formic acid
+ 3% polyoxyethylene ethylhexyl ether
+ 3% 폴리옥시에틸렌에틸헥실에테르4% formic acid
+ 3% polyoxyethylene ethylhexyl ether
상기 표 3에서 상기 3 중량%, 4 중량%의 개미산과 3 중량%의 계면활성제 혼합 조성물은 모두 95% 이상의 우수한 닭 진드기 살충효과를 나타내는 것을 확인하였으며, 상기 계면활성제에 의한 개미산의 닭 진드기 살충효력이 상승되었음을 확인하였다. 특히, 상기 4 중량% 개미산과 3 중량% 폴리옥시에틸렌옥틸페닐에테르 혼합 조성물은 평균 98.8%, 상기 4 중량% 개미산과 3 중량% 폴리옥시에틸렌에틸헥실에테르 혼합 조성물은 평균 98.5%의 탁월한 닭 진드기 살충효과를 나타내는 것을 확인하였다.In Table 3, it was confirmed that the 3% by weight, 4% by weight of formic acid and 3% by weight surfactant mixture composition all exhibited an excellent chicken mite insecticidal effect of 95% or more, and the chicken mite insecticidal efficacy of formic acid by the surfactant. It was confirmed that it was elevated. In particular, the 4% by weight formic acid and 3% by weight polyoxyethylene octyl phenyl ether mixture composition is an average of 98.8%, the 4% by weight formic acid and 3% by weight polyoxyethylene ethylhexyl ether mixture composition is an average of 98.5% excellent chicken mite pesticide It confirmed that it showed an effect.
<실시예 5> 살모넬라균 살균 시험<Example 5> Salmonella bacteria sterilization test
살모넬라균(Salmonella typhimurium)은 국립수의과학검역원으로부터 분양 받아 사용하였으며, LB 액체 배지에 연속 2 내지 3회 계대배양하여 활력이 있는 세균을 사용하였다. 온도는 37℃에서 22 내지 26시간 동안 배양한 후 사용직전까지 37℃를 유지시켰다. Salmonella typhimurium was used by pre-sale from the National Veterinary Science and Quarantine Service, and active bacteria were passaged through LB liquid medium 2-3 times in a row. The temperature was incubated at 37 ° C for 22 to 26 hours, and then maintained at 37 ° C until immediately before use.
배양된 상기 살모넬라균(1×106 cfu/mL)에 상기 실시예 3에서 제조된 조성물을 각각 투여하였다. 4시간 간격으로 LB고체 평판 배지에 100μL씩 평판 도말하여 37℃에서 배양한 후 형성된 집락을 계수하여 조성물의 농도와 노출 시간에 따른 살모넬라균의 생존율을 각각 조사하였다. 각 농도마다 평균 생존율을 3회 반복하여 조사하였다.To the cultured Salmonella (1 × 10 6 cfu / mL), each of the compositions prepared in Example 3 was administered. After plating at 100 µL on LB solid plate medium at 4 hour intervals and incubating at 37 ° C, colonies formed were counted to investigate the survival rate of Salmonella according to the composition concentration and exposure time. The average survival rate for each concentration was examined three times.
+ 3% 모노이소트리데실포스페이트
-디이소트리데실포스페이트 중합체3% formic acid
+ 3% monoisotridecylphosphate
-Diisotridecylphosphate polymer
+ 3% 모노이소트리데실포스페이트
-디이소트리데실포스페이트 중합체4% formic acid
+ 3% monoisotridecylphosphate
-Diisotridecylphosphate polymer
+ 3% 폴리옥시에틸렌옥틸페닐에테르3% formic acid
+ 3% polyoxyethylene octylphenyl ether
+ 3% 폴리옥시에틸렌옥틸페닐에테르4% formic acid
+ 3% polyoxyethylene octylphenyl ether
+ 3% 폴리옥시에틸렌트리데실에테르3% formic acid
+ 3% polyoxyethylene tridecyl ether
+ 3% 폴리옥시에틸렌트리데실에테르4% formic acid
+ 3% polyoxyethylene tridecyl ether
+ 3% 폴리옥시에틸렌에틸헥실에테르3% formic acid
+ 3% polyoxyethylene ethylhexyl ether
+ 3% 폴리옥시에틸렌에틸헥실에테르4% formic acid
+ 3% polyoxyethylene ethylhexyl ether
상기 표 4에서 3 중량%, 4 중량%의 개미산과 3 중량%의 상기 계면활성제에 대한 혼합 조성물의 살모넬라균 살균효과는 처리 12시간 이후에 모두 완전한 살모넬라균 살균효과를 나타내는 것을 확인하였다.In Table 4, it was confirmed that the Salmonella bactericidal effect of the mixed composition for 3% by weight, 4% by weight of formic acid and 3% by weight of the surfactant exhibits a complete Salmonella bactericidal effect after 12 hours of treatment.
상기 표 1 내지 4에서 나타난 바와 같이, 상기 개미산 4중량%, 계면활성제 3 중량% 및 잔량의 정제수를 혼합한 조성물은 모두 식용란에 직접 살포시 난각의 탈색 및 표면탈락 현상을 일으키지 않아 안전하고, 95% 이상의 탁월한 닭 진드기 살충효과를 나타내며, 처리 12시간 이후 살모넬라균에 완전한 살균효과를 나타내는 닭 진드기 방제조성물의 최적의 조합임을 확인하였다.As shown in Tables 1 to 4, the composition in which 4% by weight of formic acid, 3% by weight of surfactant, and the remaining amount of purified water were mixed is safe and does not cause discoloration and surface dropping of the egg shell when sprayed directly onto the edible egg, 95% It was confirmed that the optimal combination of chicken mite control composition exhibiting excellent chicken mite insecticidal effect and complete sterilization effect on Salmonella after 12 hours of treatment.
지금까지 예시적인 실시 태양을 참조하여 본 발명을 기술하여 왔지만, 본 발명이 속하는 기술 분야의 당업자는 본 발명의 범주를 벗어나지 않고서도 다양한 변화를 실시할 수 있을 것이다. 또한, 본 발명이 본 발명을 실시하는데 계획된 최상의 양식으로서 개시된 특정 실시 태양으로 국한되는 것이 아니며, 본 발명이 첨부된 특허청구의 범위에 속하는 모든 실시 태양을 포함하는 것으로 해석되어야 한다.Although the present invention has been described with reference to exemplary embodiments, those skilled in the art to which the present invention pertains may make various changes without departing from the scope of the present invention. In addition, the invention is not to be limited to the specific embodiments disclosed as the best mode planned for practicing the invention, but it should be construed that the invention includes all embodiments falling within the scope of the appended claims.
Claims (3)
상기 닭 진드기 방제조성물의 제조방법은 용매 93 내지 96.9 중량%, 개미산 3 내지 4 중량% 및 계면활성제 0.1 내지 3 중량%를 혼합하여 제조된 닭 진드기 방제조성물에 쇠비름 추출물을 더 혼합하여 제조하되,
상기 쇠비름 추출물의 추출방법은 쇠비름의 잎을 믹서기로 마쇄한 다음 상기 쇠비름잎 중량의 10배에 해당하는 메탄올을 혼합하여 45~55℃에서 24시간 방치한 후에, 여과시킨 추출용액을 45~55℃에서 회전진공감압농축기로 농축하는 방법을 사용하며,
상기 계면활성제는 모노이소트리데실포스페이트, 디이소트리데실포스페이트, 폴리옥시에틸렌옥틸페닐에테르, 폴리옥시에틸렌트리데실에테르, 폴리옥시에틸렌에틸헥실에테르로 구성된 군으로부터 선택되는 하나 또는 둘 이상의 혼합물인 것을 특징으로 하는 개미산을 이용한 저독성의 친환경 닭 진드기 방제조성물의 제조방법
In the manufacturing method of a low-toxic eco-friendly chicken mite control composition using formic acid,
The method for preparing the chicken mite control composition is prepared by further mixing purslane extract with the chicken mite control composition prepared by mixing solvents 93 to 96.9 wt%, formic acid 3 to 4 wt% and surfactant 0.1 to 3 wt%,
The method of extracting the purslane extract is ground with a blender and then mixed with methanol corresponding to 10 times the weight of purslane leaves and left at 45 to 55 ° C for 24 hours, followed by filtering the extracted extract solution at 45 to 55 ° C. The method uses the method of concentrating with a rotary vacuum decompressor.
The surfactant is one or two or more mixtures selected from the group consisting of monoisotridecyl phosphate, diisotridecyl phosphate, polyoxyethylene octylphenyl ether, polyoxyethylene tridecyl ether, and polyoxyethylene ethylhexyl ether. Low toxicity environmentally friendly chicken mite control composition using formic acid
상기 닭 진드기 방제조성물의 제조방법은 닭 진드기 방제조성물에 혼합되는 쇠비름추출물 대신에 족제비싸리 추출물을 혼합하여 제조하며,
상기 족제비싸리 추출물의 추출방법은 족제비싸리의 잎과 종자를 믹서기로 마쇄하고, 상기 족제비싸리 잎과 종자 무게의 10배의 메탄올를 혼합하여 45~55℃에서 24시간 방치한 후에, 여과시킨 추출용액을 45~55℃에서 회전진공감압농축기로 농축하는 방법을 사용하는 것을 특징으로 하는 개미산을 이용한 저독성의 친환경 닭 진드기 방제조성물의 제조방법
According to claim 2,
The method for preparing the chicken mite control composition is prepared by mixing the weasel sage extract instead of the purslane extract mixed with the chicken mite control composition,
The method of extracting the weasel fern extract was triturated with the leaves and seeds of the weasel sari with a blender, mixed with 10 times the weight of the weasel fern leaves and seeds, and left for 24 hours at 45 to 55 ° C., followed by filtering the extracted solution. Method of manufacturing a low-toxic eco-friendly chicken mite control composition using formic acid, characterized by using a method of concentrating with a rotary vacuum decompressor at 45 ~ 55 ℃.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020180128796A KR102096720B1 (en) | 2018-10-26 | 2018-10-26 | Low―toxic and eco-friendly composition for controlling Dermanyssus gallinae having Formic Acid and method for manufacturing the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020180128796A KR102096720B1 (en) | 2018-10-26 | 2018-10-26 | Low―toxic and eco-friendly composition for controlling Dermanyssus gallinae having Formic Acid and method for manufacturing the same |
Publications (1)
Publication Number | Publication Date |
---|---|
KR102096720B1 true KR102096720B1 (en) | 2020-04-02 |
Family
ID=70281734
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020180128796A KR102096720B1 (en) | 2018-10-26 | 2018-10-26 | Low―toxic and eco-friendly composition for controlling Dermanyssus gallinae having Formic Acid and method for manufacturing the same |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR102096720B1 (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20120079706A (en) * | 2011-01-05 | 2012-07-13 | 동부팜한농 주식회사 | An insecticidal composition for disinfecting stable and poultry farm |
KR20150080299A (en) * | 2013-12-31 | 2015-07-09 | 전라남도 | A composition comprising extracts of Cinnamomum camphora and Torilis japonica for inhibiting phytophthora blight |
-
2018
- 2018-10-26 KR KR1020180128796A patent/KR102096720B1/en active IP Right Grant
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20120079706A (en) * | 2011-01-05 | 2012-07-13 | 동부팜한농 주식회사 | An insecticidal composition for disinfecting stable and poultry farm |
KR20150080299A (en) * | 2013-12-31 | 2015-07-09 | 전라남도 | A composition comprising extracts of Cinnamomum camphora and Torilis japonica for inhibiting phytophthora blight |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11528908B2 (en) | Acaricide and application thereof | |
CN104322572B (en) | A kind of plant insecticide | |
EP3768087B1 (en) | Pesticidal compositions for pest control | |
EP1896045B1 (en) | Composition comprising brassicacea seeds meal and their use as biopesticides in plants | |
CN101984828A (en) | Compound pesticide | |
EP3319422B1 (en) | Compositions and methods for the control of arthropods | |
KR102096720B1 (en) | Low―toxic and eco-friendly composition for controlling Dermanyssus gallinae having Formic Acid and method for manufacturing the same | |
KR102099825B1 (en) | Compositions for controlling poultry red mite comprising extracts derived from natural materials and preparation method of the same | |
KR20210083736A (en) | Composition for acaricide containing clove oil | |
KR101400713B1 (en) | Composition of pesticide containing pesticidal essential oils and/or food additives | |
CN111374148A (en) | Insecticidal composition containing methoprene | |
KR102096721B1 (en) | Low―toxic and eco-friendly composition for controlling Dermanyssus gallinae having Oxalic Acid and method for manufacturing the same | |
CN105123767A (en) | Drosophila trap-killing agent of fruits | |
KR101632246B1 (en) | Pesticides using the Insect flower powder or sludge and manufacture method thereof | |
KR101470477B1 (en) | Composition for repelling mosquitoes | |
KR20140084596A (en) | Pesticides using the Insect flower powder or sludge | |
US20090214462A1 (en) | Cockcroach jelly bait | |
CN105165900A (en) | Biological pesticide missible oil preparation for prevention and control of forest pest Monochamus alternatus | |
KR20210083738A (en) | Composition for acaricide containing cedarwood oil | |
CN109999014A (en) | It is a kind of can the new merit chigger disease of prevention and control plants essential oil and preparation method thereof | |
CN114451427B (en) | Pesticide containing neem seed extract and copper abietate | |
KR102106921B1 (en) | The composition of livestock disinfectant which improved pest repellent effect | |
CN106719863A (en) | A kind of mosquito repellent liquid and preparation method thereof based on argy wormwood | |
KR101772693B1 (en) | Gel type composition for preventing cockroach | |
CN109452325A (en) | A kind of dacus dorsalis male and female are double to lure attractant |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant |