KR101482764B1 - Natural insecticide composition comprising botanical extract - Google Patents

Abstract

The present invention relates to a natural insecticide composition, and more specifically, to a natural insecticide including a botanical extract such as a Torilis japonica extract, a Polygonum Cuspidatum root extract, a Kalopanax pictus extract, an Amur cork-tree extract and a green tea extract as an active ingredient. The insecticide composition of the present invention is an environment-friendly formulation having no effect on the body and the environment, increases the insecticidal performance against harmful insects such as mosquitos, flies and mites, and can be utilized in various formulas.

Description

Natural insecticide composition comprising botanical extract containing plant extract as active ingredient

The present invention relates to pesticides, and more particularly, to pesticides using natural plant extracts.

Pesticides used in pest control are distinguished from digestive poison (oral), contact pesticide (percutaneous) or fumigant from the pathway in which the drug enters the pest. In recent years, hormonal agents have been developed that disturb developmental processes and are used widely as pesticides, but this creates another problem of ecosystem disturbance. In addition, the early development of infertile agents have been shown to affect and affect other organisms than insects, and they are rarely used at present.

Although the use of such insecticides is a powerful means of controlling pests, selective insecticides that exhibit high toxicity only in the target insect population have been developed, as they reduce natural enemies or cause direct and indirect harm to crops, people, and livestock. However, It also does not solve the disadvantage of acting specifically on certain bacteria or insects and adverse effects on the environment.

In addition, chemical synthetic insecticides increase insecticide resistance to pests and are environmentally unfriendly. Therefore, regulating or disturbing behavior of insect pests such as attractants that induce insect pests by sex pheromone, repellents to avoid or feeding inhibitors that inhibit feeding Although pesticides using bioactive agents have been developed, these insecticides have not been shown to have a complete pest control effect.

Therefore, it is necessary to develop a natural insecticide which is harmless to the human body and is effective in preserving natural ecosystem and preventing insect pests by preventing contamination of natural environment.

Korean Patent No. 10-1128264

The present invention has been made to solve the problems of pesticides which have been used in the past, and it has been found that the mixture of natural plant extracts extracted from dead casualties, And to provide a natural insecticide composition which is effective for pest control without being insufficient.

The present invention provides a natural insecticidal composition comprising, as an active ingredient, a mixture of plant extracts obtained by extracting casualties, horseshoe crab, Japanese apricot, Japanese apricot, and green tea.

The plant extract may contain 25 to 35% by weight of caspase extract, 15 to 25% by weight of Hojokgang extract, 15 to 25% by weight of a mandarin extract, 15 to 20% by weight of an Ehrlichia cylindrica extract and 10 to 20% .

The plant extracts may be hydrothermal, steam, ethyl acetate, chloroform, hexane, C1-C4 alcohols and mixtures thereof.

The natural pesticide composition may be selected from the group consisting of Lepidoptera, Coleoptera, Homoptera, Hemiptera, Thysanoptera, Isoptera, Orthoptera, Diptera, Hymenoptera, Siphonaptera and Acarina. ≪ Desc / Clms Page number 2 >

The natural pesticide composition may be contained in an amount of 1 to 90 parts by weight based on 100 parts by weight of the total pesticide composition.

The natural pesticide composition may be in the form of one or more formulations selected from the group consisting of suspensions, suspension emulsions, wettable powders, granular concentrates, microemulsions, wettable powders, granules, powders, emulsions, sprayed forms, .

The natural insecticidal composition of the present invention is an environmentally friendly composition which does not affect the human body and the environment and contains a mixture of plant extracts such as caspase extract, horsetail extract, mandarin extract, pricklewood extract, green tea extract and the like as an active ingredient. It can be used as a variety of formulations to increase the insecticidal power against insects such as ticks.

Fig. 1 is an extraction process for extracting the active ingredients of each plant constituting the natural insecticidal composition.

The present invention provides a natural insecticidal composition comprising, as an active ingredient, a mixture of plant extracts obtained by extracting casualties, horseshoe crab, Japanese apricot, Japanese apricot, and green tea.

More specifically, Torilis japonica is also called serpentinus, which is 30 to 70 cm in height. It has snowy hairs all over and stands straight. White flowers bloom from June to August, and bivalve inflorescence at the end of stem. It runs. Casualties are eaten as herbs, and berries are used as astringent and anti-inflammatory agents.

Polygonum cuspidata (SIEB. Et ZUCC.) Is a perennial herbaceous plant belonging to the genus Euphorbiaceae. It grows in the mountains and branches of each country, and the inside of the stem is empty, and reddish purple spots are scattered in the stem of the herd. In the oriental medicine, rootstock is mainly used for relief, diuretic and general economy.

Pine is a deciduous broad-leaved arboreous tree belonging to Araliaceae. It is distributed horizontally in the enemy country. It is used as a medicinal herb in one room.

As the deciduous tree is a deciduous tree, the bark is corky, gray, and deeply cracked. The leaves are opposite and are double leaf. It is distributed throughout Korea except Jeonnam, and it grows in the wet and fertile valley of the deep mountain, the stream. The endothelium of Echidnaeus japonica is treated with boiling water or dalyeoseo as a raw or branching agent.

The plant extract may contain 25 to 35% by weight of caspase extract, 15 to 25% by weight of Hojokgang extract, 15 to 25% by weight of a mandarin extract, 15 to 20% by weight of an Ehrlichia cylindrica extract and 10 to 20% .

More preferably, the natural insecticide composition may be formulated with 30% by weight of caspase extract, 20% by weight of Hodgkin's root extract, 20% by weight of green tea extract, 18% Do not.

The plant extracts may be hydrothermal, steam, ethyl acetate, chloroform, hexane, C1-C4 alcohols and mixtures thereof.

In addition, the plant extract according to the present invention includes not only the extract obtained by the above-mentioned extraction solvent, but also the extract obtained through a conventional purification process. For example, the extract of the present invention may contain active fractions obtained by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies, and various purification methods.

The plant extract according to the present invention can be prepared by an additional process such as vacuum concentration. In addition, the extract of the present invention can be produced in a powder state by an additional process such as vacuum distillation and freeze-drying or spray drying.

The natural pesticide composition may be selected from the group consisting of Lepidoptera, Coleoptera, Homoptera, Hemiptera, Thysanoptera, Isoptera, Orthoptera, Diptera, Hymenoptera, Siphonaptera, and Acarina. In the case of the insect,

The natural pesticide composition may be contained in an amount of 1 to 90 parts by weight based on 100 parts by weight of the total pesticide composition.

The natural pesticide composition may be in the form of one or more formulations selected from the group consisting of suspensions, suspension emulsions, wettable powders, granular concentrates, microemulsions, wettable powders, granules, powders, emulsions, sprayed forms, .

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the present invention is not limited by these examples.

< Example  1> Plant Extract Extraction Process as a Natural Pesticide Composition

1-1. Casualty extract

Torilis japonica (Houtt.) (Stem or root) was thoroughly dried and subdivided into mature water and then extracted with purified water. The hot-water extract was fermented at 50 ° C for 3 days, aged and then re-extracted with a steam distillation method. The active ingredient of the steam-extracted caspase extract was diluted with distilled water to 100 ppm and used as a sample.

1-2. Hojo Chang  extract

(Stem or root) of Reynoutria japonica HOUTT was thoroughly dried and subdivided into mature water and then extracted with purified water. The hot-water extract was fermented at 50 ° C for 3 days, aged and then re-extracted with a steam distillation method. The active ingredient of the above-mentioned steamed extract of Hojokgang extract was diluted with distilled water to 100ppm and used as a sample.

1-3. Green tea extract

Put green tea leaves in a pot heated at 250-300 ℃, spread tea leaves quickly and cool. The above procedure was repeated 3 to 4 times to completely dry the tea leaves by using a distillation method using purified water. The active ingredient of the steamed green tea extract was diluted with distilled water to 200 ppm.

1-4. Dwarf tree  extract

The bark of Phellodendron amurense was completely dried and then subdivided into mature mineral water and extracted with steam distillation method using purified water. The active ingredient of the above-mentioned steamed extract was diluted 100 ppm in distilled water and used as a sample.

1-5. Pomegranate extract

Bark of Kalapanax pictus (THUNB.) NAKAI) was thoroughly dried and subdivided into mature with mineral water and then subjected to hot water extraction with purified water. After the hot-water extract was filtered, the filtrate was fermented at 50 ° C for 3 days, aged and sonicated. A solution obtained by diluting 100 ppm of the effective substance of the above-mentioned extract with distilled water was used as a sample.

< Example  2> Production of complex plant extract

The plant extract dilutions according to Examples 1-1 to 1-5 were mixed to prepare a complex plant extract. The combined plant extracts were mixed with 30% by weight of caspase extract, 20% by weight of Hojokgang extract, 20% by weight of green tea extract, 18% by weight of Ehrlichia cylindrica, and 12% by weight of extract of mandarin.

< Example  3> Bioassay of multiple plant extracts

3-1. Identification of mite insecticidal effect

To confirm the insecticidal activity against mites, fabric-piece contact toxicity was performed. The American house dust mite (Dermatophagoides farinae) and the vertical house dust mite (Dermatophagoide pteronyssinus) were subjected to artificial insemination at 75 ± 5% relative humidity and 25 ± 5 ℃. An adequate amount of feed and saline was poured, and they were kept indoors without exposure to pesticides.

A piece of fiber having a diameter of 5 cm, which had been treated with the sample prepared in Example 2 at a concentration of 50 μM per cm 2, was placed in a Petri dish, and 30-40 individuals of each of the two mites were spun into a Petri dish and capped. After 24 hours, the condition of the mites was confirmed by using a 20-magnification microscope.

The state of the mites was judged to be the case where the mites were stimulated with fine brush or pin, and the individuals with no movement of the appendix were found dead. All the tests were repeated 3 times each, and the insecticidal activity was calculated by the following formula And the mean ± standard error as shown in Table 1.

Insecticidal activity (%) = [number of dead insects / (number of living insects + number of dead insects)] × 100

sample Insecticidal activity% (mean ± standard error) Complex plant
The extract (Example 2) Appendage Big legs house
mite Appendage Vertical house
mite 96 92 ± 2.8 98 90 ± 1.4

Referring to Table 1, it was confirmed that the compound plant extract exhibited a high insecticidal effect against the large legged house dust mite and longitudinal pattern house dust mite.

3-2. Examination of mosquito insecticidal effect

Aedes albopictus and Culex pipiens pallens larvae were kept at 25 to 28 ° C, 75 to 95% humidity and 16: 8 (L: D), respectively, in order to determine insecticidal activity against mosquitoes. The artificial diet was fed with 8: 2 mixture of poultry and yeast.

Each of the three-week-old three-week-old white mosquito bred and red mosquito larvae raised by the above method was treated with 2.5 μM of the plant extract of Example 2 for each mosquito female rats by micromachining at a rate of 30 individuals, Respectively. All the assays were repeated three times, and the insecticidal activity was calculated by the following equation and expressed as the mean ± standard error as shown in Table 2.

Insecticidal activity (%) = [number of dead insects / (number of living insects + number of dead insects)] × 100

sample Insecticidal activity% (mean ± standard error) Complex plant
The extract (Example 2) Appendage White line mosquito
(Aedes albopictus) Appendage Red house mosquito
(Culex pipiens pallens) 90 96 ± 3.3 90 100

Referring to Table 2 above, the compound plant extract showed 100% insecticidal activity against the red house mosquito larva, and the insecticidal effect against the larva of the mosquito larva was also confirmed.

3-3. Identification of housefly insecticidal effect

To confirm the insecticidal activity against the flies, 1 μl of the plant extract of Example 2 was treated per one litter of the adult female flies raised in the house, and 5 of the flies treated with the plant extract were transferred into plastic cups, (L: D) conditions under light conditions of 16: 8 (L: D) for 24 hours. All the assays were repeated three times in the above manner, and the insecticidal activity was calculated by the following equation and expressed as the mean ± standard error as shown in Table 3.

Knockdown rate (%) = [number of knocked insects / (number of insects + number of knocked insects)] × 100

sample % Knockdown% (mean ± standard error) Complex plant
The extract (Example 2 Appendage housefly 15 100

Referring to Table 3, it was confirmed that the compound plant extract of Example 2 exhibited 100% insecticidal activity against housefly.

< Example  4> Safety evaluation

4-1. One time  Dose toxicity experiment

Acute toxicity tests were performed using 7-week-old ICR mice. Ten mice were adapted to an animal feeding room for 7 days, and then 5 plants were treated with the plant extract of Example 2 and the remaining 5 mice were orally administered with water as a control. The dose of botanical extracts was orally administered at a maximum concentration of 2,000 mg / kg, and 0.05 ml of the sample was orally administered. After 14 days, the mouse was visually observed and the mice were all survived for 14 days and showed no change in body weight.

From the above results, it was confirmed that the plant extract according to the present invention is a safe substance without toxicity.

4-2. Repeated dose toxicity test

Repeated dose toxicity studies were performed using Sprague Dawley rats. Ten rats were adapted to the laboratory conditions for 14 days, and the plant extract of Example 2 was applied to the back surface of 5 mice in an experimental group, and physiological saline was applied to the back surface of 5 mice in the control group. 10% of the body surface area of the rats before application was epilated and additional epilating was performed at intervals of one week. The dose of the plant extract was 350 μl based on 1,000 mg / kg and applied for 3 days per week for 120 days. Thereafter, the animals were maintained for 28 days without treatment to confirm the recovery pattern and the persistence of the toxic effects.

As a result, the plant extract according to the present invention showed no specific abnormality in skin appearance of experimental animals. From the above results, the plant extract of the present invention was confirmed as a safe substance without toxicity.

4-3. Sensory writing experiment

Twelve guinea pig females were applied for 14 days. Three mice were treated with saline as a negative control and three were treated with Dinitrochlorobenzene (DNCB) as a positive control. In 6 experimental groups, DNCB was injected intradermally to induce first sensitization before application of the plant extract of Example 2, and 100 μl of the original extract of the plant extract was applied to the skin for 14 days to evaluate sensitization degree.

As a result, the plant extract according to the present invention did not show an allergic reaction, and as a result, it was confirmed that it was a safe substance without skin feeling.

4-4. Repeated dose inhalation toxicity test

Five specific-pathogen-free (SPF) 6-week-old rats were adapted for one week and then placed in a suction chamber and repeatedly injected with the plant extract solution of Example 2 every 6 hours in an automatic sprayer sprayed every 15 minutes Respectively. The amount of feed per day was fixed at 80g and the water was fully consumed.

The automatic injector was injected 0.15 ml per injection and about 3.75 ml per day was injected into the chamber. The body weight of the animals was measured daily for 90 days at intervals of 6 hours per day for 5 days per day under the condition that the extract was sprayed with the above method, and the state change was observed.

As a result, no significant clinical symptoms or deaths occurred in the animals inhaled with the plant extract of the present invention.

From the above results, the plant extract according to the present invention was confirmed as a safe substance.

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereby. something to do. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (6)

A natural insecticidal composition comprising, as an active ingredient, a mixture of plant extracts, each of which is extracted from casualties, horseshoe crab, apricot, oak, and green tea. The plant extract mixture according to claim 1, wherein the plant extract mixture comprises 25 to 35% by weight of the caspase extract, 15 to 25% by weight of horsetail extract, 15 to 25% by weight of the horsetail extract, 15 to 20% % &Lt; / RTI &gt; by weight of the composition. The natural insecticidal composition according to claim 1 or 2, wherein the plant extract is extracted with a solvent selected from the group consisting of hot water, steam, ethyl acetate, chloroform, hexane, C1-C4 alcohol and mixtures thereof. The natural insecticidal composition according to claim 1 or 2, wherein the natural insecticide composition is selected from the group consisting of Lepidoptera, Coleoptera, Homoptera, Hemiptera, Thysanoptera, Isoptera, Wherein the insecticidal composition is characterized by disrupting insects selected from the group consisting of Orthoptera, Diptera, Hymenoptera, Siphonaptera, Diptera and Acarina. The natural insecticidal composition according to claim 1 or 2, wherein the natural insecticide composition is contained in an amount of 1 to 90 parts by weight based on 100 parts by weight of the total insecticidal composition. 6. The composition of claim 5, wherein the natural pesticide composition is selected from the group consisting of suspension, wax emulsion, wettable powder, granular concentrate, microemulsion, wettable powder, granule, powder, emulsion, spray, &Lt; / RTI &gt; wherein the composition is at least one formulation.

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