CN105360110A - Plant extract composition having nematode and soil-borne fungi killing activity - Google Patents
Plant extract composition having nematode and soil-borne fungi killing activity Download PDFInfo
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- CN105360110A CN105360110A CN201510795264.5A CN201510795264A CN105360110A CN 105360110 A CN105360110 A CN 105360110A CN 201510795264 A CN201510795264 A CN 201510795264A CN 105360110 A CN105360110 A CN 105360110A
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- soil
- nematode
- oil
- plant
- extract composition
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
Abstract
The present invention relates to a plant extract composition having nematode and soil-borne fungi killing activity, and the plant extract composition includes the following components by weight: 2-55% of plant oil fatty acid or 5-55% of plant essential oil, 0.1-0.6 % of an antioxidant, 10-35% of a surfactant, and the balance of lactic acid. The plant extract composition having nematode and soil-borne fungi killing effect is safe, efficient, environmentally friendly, sustainable in development, and free of environmental pollution, and belongs to green environment-friendly preparations.
Description
Technical field
The present invention relates to cultural control disease field, specifically a kind of have the composition of plant extracts killing nematode and soil-borne fungus activity.
Background technology
Root-knot nematode (Meloidogyneincognita) causes the agricultural losses of about 1,570 hundred million dollars every year in the world.In China, the widest with Meloidogyne incognita distribution, endanger maximum, host's output reduction of diseased region generally reaches 15%-20%, can up to more than 70% time serious, and nematode also can cause syndrome for the infringement of crop, and namely fungal disease more easily infects the crop having infected nematode.
The control of current south China root-knot nematode still based on chemical means, such as Aldicarb, Furadan, Celfume, chloropicrin, metham-sodium etc. nematode control play an important role.But, due to height poison shortcoming and the long-term unreasonable use of synthetic chemistry bactericide itself, expose much serious problem: toxicity is large, residual high, be easy to environment, threaten human health.These high malicious chemical pesticides, directly pass to people and animals by environment and food chain etc.Therefore the new trend that high, the eco-friendly natural bactericidal agent of biologically active has become bactericide development is at present developed.
For this reason, from eco-friendly visual angle, scientist is according to the feature such as natural plant active component " toxicity is little ", " decomposing fast " and " polluting few ", and the various plant resource meeting organic farming requirement of active development kills line preparation.
But natural plant active component shows higher hydrophobicity mostly, suitable water-soluble dosage forms must be developed and can drop into practical application.But existing research many employings ethanol, acetone and other organic solvent when preparation, as the carrier systems of active component, still there is certain toxicity, and physical stability are poor, or scale organic farming production can not cannot be applied to.
Summary of the invention
Object of the present invention is just for above-mentioned the deficiencies in the prior art, aim to provide and a kind of there is the composition of plant extracts killing nematode and soil-borne fungus activity, its active component is all from natural plant extracts, and environmentally safe, belongs to environmental type preparation.
Technical scheme provided by the invention comprises the composition of following percentage by weight: vegetable fatty acids 2-55% or plants essential oil 5-55%, antioxidant 0.1-0.6%, surfactant 10-35%, and surplus is lactic acid.
Wherein, described vegetable oil is one or more in cocoa butter, Canola oil, kostelezkya virginica seed oil.
Wherein, described antioxidant is TBHQ (TBHQ), butylated hydroxy anisole (BHA), one or more in 2,6-toluene di-tert-butyl phenol (BHT).
Wherein, surfactant is one or both in Dofax111 and 111.
The invention provides a kind of highly effective and safe, environmental friendliness, can continue development there is the composition killing soil-borne fungus, nematode effect, and these compositions are micro-emulsifiers, significantly can increase active ingredient solubility, improve its bioavilability, active component of the present invention is all from natural plant extracts, environmentally safe, belongs to environmental type preparation.
Embodiment
Below in conjunction with embodiment, specific embodiment of the invention method is further described.This embodiment is used for clearly technical scheme of the present invention being described, and can not limit the scope of the invention with this.
The preferred embodiments of the present invention preparation group is as follows: (by weight percentage)
| Component (W/W%) | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Canola oil | 55% | - | - |
| Cocoa butter | - | 55% | - |
| Kostelezkya virginica oil | - | - | 55% |
| Lactic acid | 10% | 10% | 10% |
| Surfactant | 35% | 35% | 35% |
| 4 DEG C of cold storages 1 week | Qualified | Qualified | Qualified |
| 54 DEG C of heat are store 2 weeks | Qualified | Qualified | Qualified |
Above-mentioned preparation is respectively used to test, specific embodiment of the invention process to be described and verification technique advantageous effect.
(1) MEC-8910 and MEC-1003 is to the isolated experiment of Meloidogyne incognita
1, Meloidogyne incognita (
meloidogyneincognica) preparation of suspension
Get the old complaint infecting root-knot nematode, after cleaning, be placed in 500 order mesh screens immediately and constantly eluriate for several times, the ovum on flushing sieve is in beaker, and constant volume is for subsequent use.Get 1ml root-knot nematode second instar larvae suspension, containing root-knot nematode second instar larvae 90-130 bar, detect J2 under stereomicroscope and to live borer population, add medicament, observe when making nematode and medicament time of contact be 30min and remain nematode work borer population.
2, observation, statistical data
By Abbott formulae discovery corrected mortality, according to log concentration and dead probit value, calculate the virulence regression equation y=ax+b of medicament, semi lethal concentration (EC
50) and EC
90coefficient R.
MEC-1003 and MEC-8910 processes the virulence after in vitro root-knot nematode 0.5h respectively
| Virulence regression equation | LC50(mg/L) | LC90(mg/L) | Coefficient R | |
| Canola oil | Y=-5.3632＋3.9061X | 450 | 957.6 | 0.9983 |
| Cocoa butter | Y=-2.4133+2.8855X | 273.0 | 729.5 | 0.9943 |
| Kostelezkya virginica oil | Y= -3.7207+3.5586X | 257.1 | 583.8 | 0.9956 |
(2) to root-knot nematode egg and the potted plant test of pesticide effectiveness of second instar larvae
1, the acquisition of tomato seedling (3-4 leaf): vernalization is to showing money or valuables one carries unintentionally in conventional manner for tomato seeds, and be seeded in seedling-growing container that diameter is 5cm, culture soil quality (sand: soil=2:1), greenhouse temperature (18-34 DEG C), one, every ware, transplants during 4-5 leaf.
2, cut-off footpath is that 6cm nutritive cube carries out following pretreatment, MEC-1003250 ×, MEC-1003500 ×, MEC-10031000 ×, MEC-8910250 ×, MEC-8910500 ×, Avermectin 2000 ×.Wherein twice pretreatment interval time is one week: every basin formulation rate is 40ml, after each process terminates, transplants tomato plants (4-5 leaf) after one week.
3, data survey and record: record each root system insect gall number, and classification:
Zero level: strong seedling anosis (without root knot on root system);
One-level: less than 10% root system has root knot, but root knot does not connect mutually;
Secondary: the root system of 11% ~ 30% has root knot, only a small amount of root knot is interconnected:
The root system of three grades: 31% ~ 50% has root knot, and the following root knot of half is interconnected;
Level Four: the root system of 51% ~ 75% has root knot, root knot more than half is interconnected, and part is main, side root is thicker in deformity;
Pyatyi: the root system of more than 75% has root knot, and is interconnected, Duo Shuozhu, side root are thicker in deformity.
4, data processing.Calculate each process insect gall index, then compare insect gall index and plant growth parameters relationship, relatively more each impact processed plant growth and nematode, evaluates various medicament drug effect.
Disease index=∑ (diseased plant number at different levels × corresponding number of levels)/(investigating total strain number × highest number of levels) × 100%;
Relative control effect=(contrast disease index-process disease index)/contrast disease index × 100%.
three embodiments are to the potted plant drug effect of second instar larvae:
| Medicine | Concentration (ppm) | A pretreatment preventive effect (%) | Secondary pretreatment preventive effect (%) |
| Canola oil | 4000 | 93.33 | 92 |
| 2000 | 80.00 | 80.00 | |
| Cocoa butter | 4000 | 80.00 | 73.33 |
| 2000 | 68.34 | 53.33 | |
| Kostelezkya virginica oil | 4000 | 76.67 | 60.00 |
| 2000 | 62.23 | 51.12 |
(3) indoor biometrics of four kinds of disease funguses is tested
Test Rhizoctonia solani (
rhizoctoniasolani), citrus anthracnose bacterium (
colletotrichumgloeosporioides), Fusarium oxysporum (
fusariumoxysporium), cucumber corruption mould (
pythiumaphanidermatum(Edson)
fitzp.) indoor harmacological effect, these four kinds of disease funguses are universal agricultural diseases, and host range is extensive, all kinds of crops can be caused to rot, damping off, and the time that disease seriously occurs can cause a large amount of dead seedlings, causes heavily to plant, reseed, ruin kind, plant growth period is postponed, and causes a large amount of underproduction.
1, test method and step:
Growth hormone rate determination method is adopted to detect.1000mgL-1 mother liquor will be mixed with for reagent thing aseptic deionized water, and then be diluted to the liquid of 5 ~ 6 concentration gradients, now with the current.Get and be added in 9mLPDA medium for reagent liquid 1mL, pour culture dish into, place 1 bacterium cake (diameter is 6mm) in each culture dish central authorities, take sterile water as solvent control, each process repeats 3 times.Be placed in 28 DEG C of constant temperature culture, observed and recorded mycelial growth situation after 72 hours, right-angled intersection method measures colony growth diameter, with following formulae discovery mycelial growth inhibition rate:
Mycelial growth inhibition rate (%)=(contrast colony diameter-bacterium cake diameter)-(contrast colony diameter-bacterium cake diameter)/(process colony diameter-bacterium cake diameter) * 100.
2, result of the test
| Drug concentration (ppm) | Rhizoctonia solani Kuhn inhibiting rate | Citrus anthracnose bacterium inhibiting rate | Fusarium oxysporum inhibiting rate | |
| Canola oil | 250 | 62.8% | 45.5% | 35.8% |
| 500 | 78.4% | 89.7% | 77.5% | |
| 1000 | 100% | 98.5% | 95.4% | |
| Cocoa butter | 250 | 60.0% | 66.8% | 67.8% |
| 500 | 88.9% | 85.3% | 82.9% | |
| 1000 | 100% | 100% | 100% | |
| Kostelezkya virginica oil | 250 | 55.7% | 44.8% | 56.9% |
| 500 | 70.2% | 67.7% | 60.7% | |
| 1000 | 89.5% | 94.3% | 92.1% |
Above example illustrates, the sterilization of the present composition and kill nematode Be very effective.
The present invention is the achievement that above-mentioned three Plant Extracts of research obtain the control effects of various pathogen and soil nematodes.
The vegetable oil used in the present invention is cocoa butter, kostelezkya virginica seed oil, Canola oil one or more, wherein:
That the shell that coconut (CocosnuciferaL.) is fiber by skin forms, coconut coir is provided and includes the large nut of edible thick meat.Cocoa butter is the edible oil of a kind of extraction in ripe coconut palm pulp.Cocoa butter is at food manufacturing, medicine and industrially have multiple use, highly stable to heat, therefore it to have oxidation slow, the antiacid feature lost.Coconut oil-containing 35%-45%.Containing free fatty acid 20% in oil, caproic acid (caproicacid) 2%, palmitic acid (palmiticacid), caprylic acid (caprylicacid) 9%, capric acid (capricacid), oleic acid (oleicacid) 2%, lauric acid (lauricacid).
Kostelezkya virginica (Kosteletzkyavirginica) is Malvaceae herbaceos perennial, it is the pioneer's salt-tolerant plant being adapted to beach strongly salined soil environment, kostelezkya virginica fleshy root output can up to 15000kg/hm2, wherein polysaccharide (about about 5.36%), flavones (about 0.72%), saponin(e (about 0.62%) isoreactivity content of material enrich, and the potentiality to be exploited as food (feed), energy-source plant, landscape plant is very large.Kostelezkya virginica seed oil content more than 18%, protein more than 20%, equals to soybean.
Rape (BrassicacampestrisL.) is Cruciferae, brassica plant, and multiple species of seed oil-containing in plant are referred to as rape by agronomy.Rape is nutritious, and wherein Vitamin C content is very high.Rape seed can be extracted oil, containing multivitamin in Canola oil, as vitamin A, vitamin D and vitamin E, wherein content of vitamin E reaches 60 milligrams/100 grams oil, especially A type content of vitamin E up to 13.2 milligrams/100 grams of oil, for 2.58 times of soybean oil, and reduce few after long term storage and heating, can be used as the source of food vitamins E.It is the important sources of human body lipovitamin.Content of phytosterol in Canola oil also comparatively the Vegetable Oils such as soya-bean oil be high, and of a great variety, some sterol also has special physiological function.
The extract of above-mentioned three Plants all has the effect of killing two spotted spider mite, and their environmental toxicities are low, clearly indicates avoid registration at U.S. EPA 40CFR152:25B.
In addition, vegetable oil and other component are mixed and made into microemulsifying formulations by the present invention, are convenient to be applied to two spotted spider mite and control.Surfactant makes the vegetable oil microemulsion that dispersed formation is stable in water, and solvent plays diluting effect.And the solvent that the present invention uses is for being low VOC solvent, to ecotope without harm.
Therefore, the invention solves the technical problem that vegetable oil can not be applied to soil, provide a kind of composition having sterilization simultaneously and kill nematode double action, it is not containing toxicity, and environmentally safe, belongs to environmental type preparation.
Above are only the preferred embodiment of the present invention, be to be noted that for the ordinary skill technical staff in the industry, can be improved by some and replace under principle of the present invention, this improvement and replacement also should be considered as protection scope of the present invention.
Claims (3)
1. one kind has the composition of plant extracts killing nematode and soil-borne fungus activity, it is characterized in that the raw material comprising following percentage by weight: vegetable fatty acids 2-55% or plants essential oil 5-55%, antioxidant 0.1-0.6%, surfactant 10-35%, surplus is lactic acid.
2. according to claim 1 a kind ofly have the composition of plant extracts killing nematode and soil-borne fungus activity, it is characterized in that: described vegetable oil is one or more in cocoa butter, Canola oil, kostelezkya virginica seed oil.
3. according to claim 1 a kind ofly have the composition of plant extracts killing nematode and soil-borne fungus activity, it is characterized in that: described antioxidant is TBHQ (TBHQ), butylated hydroxy anisole (BHA), one or more in 2,6-toluene di-tert-butyl phenol (BHT).
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|---|---|---|---|
| CN201510795264.5A CN105360110A (en) | 2015-11-17 | 2015-11-17 | Plant extract composition having nematode and soil-borne fungi killing activity |
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|---|---|---|---|
| CN201510795264.5A CN105360110A (en) | 2015-11-17 | 2015-11-17 | Plant extract composition having nematode and soil-borne fungi killing activity |
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| CN105360110A true CN105360110A (en) | 2016-03-02 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1800346A (en) * | 2005-12-16 | 2006-07-12 | 沈阳农业大学 | Fungus possessing eelworm-killing activity and its preparation method and uses |
| CN101356933A (en) * | 2008-08-05 | 2009-02-04 | 聊城大学 | Edible vegetable oil bactericide and use method thereof |
| CN101558771A (en) * | 2009-05-26 | 2009-10-21 | 刘兴健 | Active pesticide |
| CN103271045A (en) * | 2013-07-02 | 2013-09-04 | 中国农业科学院植物保护研究所 | Composition and method for preventing and treating root-knot nematode, soil borne pathogenic fungi and weeds |
| CN103783093A (en) * | 2014-03-13 | 2014-05-14 | 四川龙蟒福生科技有限责任公司 | Composition having functions of sterilization and nematode prevention and control |
-
2015
- 2015-11-17 CN CN201510795264.5A patent/CN105360110A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1800346A (en) * | 2005-12-16 | 2006-07-12 | 沈阳农业大学 | Fungus possessing eelworm-killing activity and its preparation method and uses |
| CN101356933A (en) * | 2008-08-05 | 2009-02-04 | 聊城大学 | Edible vegetable oil bactericide and use method thereof |
| CN101558771A (en) * | 2009-05-26 | 2009-10-21 | 刘兴健 | Active pesticide |
| CN103271045A (en) * | 2013-07-02 | 2013-09-04 | 中国农业科学院植物保护研究所 | Composition and method for preventing and treating root-knot nematode, soil borne pathogenic fungi and weeds |
| CN103783093A (en) * | 2014-03-13 | 2014-05-14 | 四川龙蟒福生科技有限责任公司 | Composition having functions of sterilization and nematode prevention and control |
Non-Patent Citations (1)
| Title |
|---|
| EL-KADY等: "Formulation and evaluation the nematicidal activity of certain plant oils against citrus nematode Tylenculus semipenetrans", 《ARAB UNIVERSITIES JOURNAL OF AGRICULTURAL SCIENCES》 * |
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