CN103271045A - Composition and method for preventing and treating root-knot nematode, soil borne pathogenic fungi and weeds - Google Patents
Composition and method for preventing and treating root-knot nematode, soil borne pathogenic fungi and weeds Download PDFInfo
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- CN103271045A CN103271045A CN2013101845640A CN201310184564A CN103271045A CN 103271045 A CN103271045 A CN 103271045A CN 2013101845640 A CN2013101845640 A CN 2013101845640A CN 201310184564 A CN201310184564 A CN 201310184564A CN 103271045 A CN103271045 A CN 103271045A
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Abstract
The invention discloses a composition and a method for preventing and treating root-knot nematode, soil borne pathogenic fungi and weeds, and belongs to the technical field of pesticides. The composition consists of dazomet preparation and dimethyl disulfide preparation. The using method of the composition comprises the following steps of: performing deep tillage on the soil, then applying the dazomet preparation into the soil, injecting the dimethyl disulfide preparation into the soil for applying the dimethyl disulfide preparation into the soil through a dropping system, covering a film on the soil by a reverse burying method, fumigating the soil for 2-4 weeks, ventilating the soil for 3-12 days, and planting a crop seed to be cultivated into the soil after the soil does not contain any insecticide residues based on the qualitative judgment of a sprouting test. The composition is suitable for sterilization of the soil before vegetables, strawberries, flowers, melons, nursery gardens, fruit gardens, tea gardens, grape gardens, forests, traditional Chinese medicinal crops and the like are planted; and therefore, the southern root-knot nematode, the main soil borne pathogenic fungi and the main graminaceous and broad leaf weeds can be effectively prevented and treated.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of composition and method of preventing and treating root-knot nematode, soil-borne disease fungal pathogens and weeds.
Background technology
Over past ten years, along with the adjustment of agricultural planting structure, protect the ground cultivation to have in China and develop by leaps and bounds.Protection ground, whole nation area is above 2,000,000 hectares.And the cultivation of protection ground is increased farmers' income as one in China; by the important agricultural measures of traditional farming to the modern agriculture transformation; to continue development in future, the crop of cultivation also will be developed to directions such as flowers, melon, fruit tree, nursery, Chinese herbal medicines by current vegetables, strawberry.The development on protection ground; also for the generations of root-knot nematode, soil-borne disease and weeds, development provide adapt circumstance, plant 3～5 years usually after, the yield and quality of crop is seriously influenced; the general underproduction 20～40%, the serious underproduction is 60% or more even have no harvest.Along with the continuation development on protection ground and the cultivation year after year of high added value crop, the problem of root-knot nematode, soil-borne disease and weeds will be more and more outstanding, and will become the key factor of serious restriction protection ground development.According to the inventor in Shandong, ground investigation such as Jiangsu, Liaoning, Hebei, Zhejiang, Henan, the harm of root-knot nematode, soil-borne disease and weeds has become the outstanding problem in the cultivation of protection ground.Because no effectively preventing method, the ground peasant uses the medicament (as Aldicarb, carbofuran, thimet etc.) of country's forbidding to prevent and treat root-knot nematode, soil-borne disease and weeds etc., serious harm people's life and health in a large number in Shandong etc.Because protection ground investment is big, some processing times are long, need longer crop rotation time method to be difficult to carry out in the greenhouse, also are difficult to be accepted by the peasant.And the soil chemistry disinfection technology becomes one of one preferred technique of current protection ground agriculture plant protection just day by day before planting.
Summary of the invention
The present invention is directed to easily take root in the economic crops such as present flowers, melon, fruit tree, nursery, Chinese herbal medicine tie lines worm, soil-borne disease and weeds, and the problem that is difficult to eradicate, a kind of composition of preventing and treating root-knot nematode, soil-borne disease fungal pathogens and weeds is proposed.
The present invention also aims to provide a kind of using method of preventing and treating the composition of root-knot nematode, soil-borne disease fungal pathogens and weeds.
A kind of composition of preventing and treating root-knot nematode, soil-borne disease fungal pathogens and weeds, said composition is made up of dazomet preparation and Methyl disulfide preparation.
Described dazomet preparation is for being the formulations of active ingredients product with the dazomet.
Described dazomet preparation is 98% dazomet fine granule.
Described Methyl disulfide preparation is for being the formulations of active ingredients product with the Methyl disulfide.
Described Methyl disulfide preparation is 99% Methyl disulfide.
A kind of control root-knot nematode, soil-borne disease fungal pathogens and method for weed, carry out according to following steps:
(1) at first soil is ploughed deeply 25-35cm, then by active ingredient 20-30g/m
2Consumption, the dazomet preparation is mixed soil uses;
(2) secondly, the Methyl disulfide preparation is pressed active ingredient 35-45g/m
2Consumption, be injected into soil or be applied in the soil by drip irrigation system, the degree of depth is 20-30cm;
(3) last, adopt the anti-method overlay film that buries after stifling 2～4 weeks, spacious gas 3-12 days, after treating that the no medicament of raise crop seed germination test qualitative judgement is residual, can plant.
Described crop is vegetables, strawberry, flowers, melon, fruit tree, nursery or Chinese herbal medicine.
Beneficial effect of the present invention: composition of the present invention is to Meloidogyne incognita, reaping hook belongs to fungi, the Phytophthora fungi, broad leaved weeds (as piemarker etc.) and grassy weed (as lady's-grass etc.) all have obvious synergistic effect, use conveniently, to crop safety, simultaneously, two kinds of medicaments all do not have residual in soil, to crop safety, composition for preventing and controlling Meloidogyne incognita of the present invention, reaping hook belongs to fungi, the Phytophthora fungi, broad leaved weeds and grassy weed effect excellence, and use cost is well below soil fumigant Celfume commonly used etc., and need not look like to use the special heating vaporising devices of employing such as Celfume, therefore save a large amount of the expenditure; Chemical fumigant is generally high malicious chemicals, and one of composition of the present invention dazomet low toxicity plays a role by producing methyl-isorhodanate (MITC), and another composition Methyl disulfide is poisoned, and the two all takes soil application in addition, therefore to the person poultry safety.
Related composition of the present invention is applicable to that Meloidogyne incognita, the reaping hook on the crops such as control vegetables, strawberry, flowers, melon, Chinese herbal medicine, nursery, orchard, tea place, forest belongs to fungi, Phytophthora fungi, broad leaved weeds and grassy weed etc.
Description of drawings
Fig. 1 handles Fusarium effect comparison diagram for each.
Fig. 2 handles piemarker effect comparison diagram for each.
Fig. 3 handles lady's-grass effect comparison diagram for each.
Embodiment
The present invention will be further described below in conjunction with the drawings and specific embodiments.
The reagent that present embodiment adopts is:
99% Methyl disulfide (Shanghai Yuan Ji chemical industry Co., Ltd), abbreviation: DMDS;
98% dazomet fine granule (strong chemical industry Co., Ltd is executed in the Nantong), abbreviation: DZ.
For the examination soil sample be:
Soil sample is picked up from Tongzhou, Beijing cucumber ground of uprooting plants after their edible portions have been harvested, and nematode and disease take place seriously throughout the year, and after getting topsoil and crossing the 2mm sieve, mixing is standby.By analysis, soil moisture content (water: dry ground) be 19.84%, pH7.07, soil organic matter content 33.0g/kg, NH
4+-N37.2mg/kg, NO
3-N388.1mg/kg, available phosphorus 327.7mg/kg, available potassium 875.4mg/kg belongs to sandy loam.
Evaluation index and method are:
Meloidogyne incognita Meloidogyne incognita. adopts centrifugal process;
Fusarium Fusarium spp. adopts the Komada method to separate;
Phytophthora belongs to Phytophthora spp., adopts the Masago method to separate;
Piemarker Abutilon theophrasti adopts the vernier caliper measurement bud long;
Lady's-grass Digitaria sanguinalis adopts the vernier caliper measurement bud long.
Test method
1, dose setting
By dazomet (active ingredient) and two concentration for the treatment of 100mg/kg of soil quality ratio design and 50mg/kg (the corresponding product quality is 61.2mg/600g, 30.6mg/600g);
By DMDS (active ingredient) and two concentration for the treatment of 40mg/kg of soil quality ratio design and 20mg/kg (the corresponding product volume is 23.04uL/600g, 11.52uL/600g, and 99% DMDS density is 1.052g/mL);
Each handles as follows:
2, process of the test
Take by weighing the ready soil sample of 600g, put into the 2.5L drier.During dispenser, dazomet fine granule use ten thousand/take by weighing, with soil sample mixing in the drier; DMDS accurately pipettes with liquid-transfering gun.After applying medicament, each handles rapid program request respectively 10 in 15 in the lady's-grass seed of vernalization and piemarker seed.25 ℃ of down stifling 5d.The stifling spacious gas 1d in back that finishes separates nematode and soil-borne disease fungal pathogens then, and the bud of measuring lady's-grass, piemarker simultaneously is long.And space management is set, each is handled 3 times and repeats.
3, computational methods
Root-knot nematode survival rate and corrected mortality calculate with following formula:
Survival rate %=100 * keep nematode number/confession to try total borer population
Corrected mortality %=100 * (handling lethality-contrast lethality)/(1-contrasts lethality)
The pathogen inhibiting rate calculates with following formula:
Inhibiting rate %=100 * (check plot pathogen quantity-chemicals treatment district pathogen quantity)/check plot pathogen quantity
The weeds inhibiting rate calculates with following formula:
Inhibiting rate %=100 * (blank weeds bud length-chemicals treatment weeds bud is long)/blank weeds bud is long
According to Colby method (1966) formula calculate preventive effect and with the preventive effect of actual measurement relatively, easy, estimate the synergy effect of mixture effectively.Computing formula is:
E＝X
1×X
2...×X
n/100
(n-1)
E is the theoretical preventive effect of mixture; N is the quantity of using medicament with; X
1The 1st kind of preventive effect behind the medicament used in expression; X
2The 2nd kind of preventive effect behind the medicament medicament used in expression; X
nThe preventive effect behind the n kind medicament is used in expression.When the actual preventive effect of mixture during greater than theoretical preventive effect, expression synergy; When the actual preventive effect of mixture during less than theoretical preventive effect, the expression antagonism.
4, measurement result
Effect to root-knot nematode
Table 1 Methyl disulfide+dazomet is used the effect to Meloidogyne incognita with
As can be seen from Table 1, four of Methyl disulfide and dazomet kinds of different amounts are used with root-knot nematode have all been shown synergistic effect.The Meloidogyne incognita corrected mortality that four kinds of different amounts of Methyl disulfide and dazomet are used with has all reached more than 83%, reaches more than 93% when the high consumption of Methyl disulfide and the high consumption of dazomet are used with, and it is obvious to use synergy with.
Effect to two kinds of main soil-borne disease fungal pathogenses
Table 2 Methyl disulfide+dazomet is used the effect to two kinds of main soil-borne disease fungal pathogenses with
From table 2 and Fig. 1 as can be seen, four kinds of different amounts of Methyl disulfide and dazomet are used with Fusarium and phytophthora belonged to and have all been shown synergistic effect.Four kinds of different amounts of Methyl disulfide and dazomet are used with the Fusarium inhibiting rate are all reached more than 90%, and the inhibiting rate that phytophthora is belonged to all reaches more than 88%, and it is obvious to use synergy with.
Effect to two kinds of main weeds in field
Table 3 Methyl disulfide+dazomet is used the effect to two kinds of main weeds in field with
By table 3, Fig. 2 and Fig. 3 as can be seen, four of Methyl disulfide and dazomet kinds of different amounts are used with piemarker and lady's-grass have all been shown synergistic effect.Use with except the low consumption of Methyl disulfide and the low consumption of dazomet piemarker and lady's-grass inhibiting rate are respectively 95.98% and 99.64%, its excess-three is used processing with piemarker and lady's-grass inhibiting rate is all reached 100%, illustrates that both pharmacy mix all have fabulous inhibitory action to broad leaved weeds and grassy weed.
Before lettuce planting, at first soil is ploughed deeply 30cm, guarantee that simultaneously soil has certain humidity, then 98% dazomet fine granule (is pressed active ingredient 25g/m
2Consumption) mix soil and use, afterwards with 99% Methyl disulfide (by active ingredient 40g/m
2Consumption) evenly be injected in the soil, adopt " the anti-method of burying " overlay film after stifling 2 weeks at last, spacious gas 3 days, by the lactuca sativa seeds sprouting test qualitatively judge no medicament residual after, can plant.
Before green cucumber, at first soil is ploughed deeply 30cm, guarantee that simultaneously soil has certain humidity, then 98% dazomet fine granule (is pressed active ingredient 25g/m
2Consumption) mix soil and use, afterwards with 99% Methyl disulfide (by active ingredient 40g/m
2Consumption) evenly be applied in the soil (for increasing the water-soluble of Methyl disulfide by drip irrigation system, need the amount by 4% to add Tween 80), adopt " the anti-method of burying " overlay film after stifling 3 weeks at last, spacious gas 7 days, after residual by the no medicament of germination of cucumber seeds test qualitative judgement, can plant.
Before the tulip plantation, at first soil is ploughed deeply 35cm, guarantee that simultaneously soil has certain humidity, then 98% dazomet fine granule (is pressed active ingredient 20g/m
2Consumption) mix soil and use, afterwards with 99% Methyl disulfide (by active ingredient 35g/m
2Consumption) evenly be applied in the soil (for increasing the water-soluble of Methyl disulfide by drip irrigation system, need the amount by 4% to add Tween 80), adopt " the anti-method of burying " overlay film after stifling 4 weeks at last, spacious gas 10 days, after residual by the no medicament of tulip seed germination test qualitative judgement, can plant.
Before root of Dahurain angelica plantation, at first soil is ploughed deeply 25cm, guarantee that simultaneously soil has certain humidity, then 98% dazomet fine granule (is pressed active ingredient 30g/m
2Consumption) mix soil and use, afterwards with 99% Methyl disulfide (by active ingredient 45g/m
2Consumption) evenly be injected in the soil, adopt " the anti-method of burying " overlay film after stifling 2 weeks at last, spacious gas 5 days, by the test of root of Dahurain angelica seed germination qualitatively judge no medicament residual after, can plant.
Before strawberry cultivating, at first soil is ploughed deeply 28cm, guarantee that simultaneously soil has certain humidity, then 98% dazomet fine granule (is pressed active ingredient 27g/m
2Consumption) mix soil and use, afterwards with 99% Methyl disulfide (by active ingredient 41g/m
2Consumption) evenly be injected in the soil, adopt " the anti-method of burying " overlay film after stifling 3 weeks at last, spacious gas 10 days, by the test of strawberry seed germination qualitatively judge no medicament residual after, can plant.
Before the tealeaves plantation, at first soil is ploughed deeply 35cm, guarantee that simultaneously soil has certain humidity, then 98% dazomet fine granule (is pressed active ingredient 29g/m
2Consumption) mix soil and use, afterwards with 99% Methyl disulfide (by active ingredient 38g/m
2Consumption) evenly be injected in the soil, adopt " the anti-method of burying " overlay film after stifling 3 weeks at last, spacious gas 10 days, by the test of tealeaves seed germination qualitatively judge no medicament residual after, can plant.
Claims (7)
1. a composition of preventing and treating root-knot nematode, soil-borne disease fungal pathogens and weeds is characterized in that, said composition is made up of dazomet preparation and Methyl disulfide preparation.
2. according to the composition of the described control of claim 1 root-knot nematode, soil-borne disease fungal pathogens and weeds, it is characterized in that described dazomet preparation is for being the formulations of active ingredients product with the dazomet.
3. according to the composition of the described control of claim 2 root-knot nematode, soil-borne disease fungal pathogens and weeds, it is characterized in that described dazomet preparation is 98% dazomet fine granule.
4. according to the composition of the described control of claim 1 root-knot nematode, soil-borne disease fungal pathogens and weeds, it is characterized in that described Methyl disulfide preparation is for being the formulations of active ingredients product with the Methyl disulfide.
5. according to the composition of the described control of claim 4 root-knot nematode, soil-borne disease fungal pathogens and weeds, it is characterized in that described Methyl disulfide preparation is 99% Methyl disulfide.
6. a control root-knot nematode, soil-borne disease fungal pathogens and method for weed is characterized in that, carry out according to following steps:
(1) at first soil is ploughed deeply 25-35cm, then by active ingredient 20-30g/m
2Consumption, the dazomet preparation is mixed soil uses;
(2) secondly, the Methyl disulfide preparation is pressed active ingredient 35-45g/m
2Consumption, be injected into soil or be applied in the soil by drip irrigation system, the degree of depth is 20-30cm;
(3) last, adopt the anti-method overlay film that buries after stifling 2～4 weeks, spacious gas 3-12 days, after treating that the no medicament of raise crop seed germination test qualitative judgement is residual, can plant.
7. according to the described a kind of control root-knot nematode of claim 6, soil-borne disease fungal pathogens and method for weed, it is characterized in that described crop is vegetables, strawberry, flowers, melon, fruit tree, nursery or Chinese herbal medicine.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105360110A (en) * | 2015-11-17 | 2016-03-02 | 安徽云溪农业有限公司 | Plant extract composition having nematode and soil-borne fungi killing activity |
| CN106561679A (en) * | 2016-11-11 | 2017-04-19 | 中国农业科学院植物保护研究所 | Pesticide composition and application thereof |
| CN108293705A (en) * | 2016-08-24 | 2018-07-20 | 文山苗乡三七科技有限公司 | A method of handling notoginseng soil with soil fumigant dazomet |
| CN114097346A (en) * | 2021-11-30 | 2022-03-01 | 李守华 | Film-covering high-temperature fumigation soil remediation method for solving ginger continuous cropping obstacle |
| CN114391537A (en) * | 2022-01-27 | 2022-04-26 | 中国农业科学院植物保护研究所 | Pharmaceutical composition, medicine and application in prevention and treatment of nematode diseases |
| CN115315183A (en) * | 2020-03-24 | 2022-11-08 | 塔明克私人有限公司 | Method for reducing fusarium contamination |
Citations (2)
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| CN1124573A (en) * | 1994-12-12 | 1996-06-19 | 桂保华 | Sterilizing agent for seeds and soil |
| WO2004077949A1 (en) * | 2003-03-05 | 2004-09-16 | Kanesho Soil Treatment Bvba | Compositions for controlling plant-injurious organisms |
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2013
- 2013-07-02 CN CN201310184564.0A patent/CN103271045B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1124573A (en) * | 1994-12-12 | 1996-06-19 | 桂保华 | Sterilizing agent for seeds and soil |
| WO2004077949A1 (en) * | 2003-03-05 | 2004-09-16 | Kanesho Soil Treatment Bvba | Compositions for controlling plant-injurious organisms |
Non-Patent Citations (2)
| Title |
|---|
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105360110A (en) * | 2015-11-17 | 2016-03-02 | 安徽云溪农业有限公司 | Plant extract composition having nematode and soil-borne fungi killing activity |
| CN108293705A (en) * | 2016-08-24 | 2018-07-20 | 文山苗乡三七科技有限公司 | A method of handling notoginseng soil with soil fumigant dazomet |
| CN106561679A (en) * | 2016-11-11 | 2017-04-19 | 中国农业科学院植物保护研究所 | Pesticide composition and application thereof |
| CN106561679B (en) * | 2016-11-11 | 2020-01-07 | 中国农业科学院植物保护研究所 | Pesticide composition and application thereof |
| CN115315183A (en) * | 2020-03-24 | 2022-11-08 | 塔明克私人有限公司 | Method for reducing fusarium contamination |
| CN114097346A (en) * | 2021-11-30 | 2022-03-01 | 李守华 | Film-covering high-temperature fumigation soil remediation method for solving ginger continuous cropping obstacle |
| CN114391537A (en) * | 2022-01-27 | 2022-04-26 | 中国农业科学院植物保护研究所 | Pharmaceutical composition, medicine and application in prevention and treatment of nematode diseases |
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| CN103271045B (en) | 2015-02-25 |
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