KR102255693B1 - Cultivation method of Cissus quadrangularis - Google Patents

Abstract

The present invention relates to a cultivation method of Cissus quadrangularis, and more particularly, to a cultivation method of Cissus quadrangularis with a high active ingredient content in a short time without pesticide spraying or fertilization after planting by planting of the cissus seedlings in soil after transplanting and cultivating the cissus seedlings several times using a uniformly composed soil composition. The Cissus cultivation method of the present invention comprises: a step of preparing the cissus seedlings; a pot cutting cultivation step of cutting and cultivating the cissus seedlings in a pot containing the soil composition for cultivating the cissus; a pot transplant-cultivation step of transplanting and cultivating the same 1 to 4 times in a larger pot containing the soil composition for the cissus cultivation; and a soil cultivation step of planting and cultivating the same in the soil after the pot-transplant cultivation step is completed.

Description

Cultivation method of Cissus quadrangularis}

The present invention relates to a method of cultivation of Sisus, and more specifically, by transplanting and cultivating Sisus seedlings several times using a uniformly formulated soil composition, and then planting it in the soil, in a short period of time without pesticide spraying or fertilization after planting. It relates to a method for cultivating cissus with a high content of active ingredients.

Cissus (Cissus quadrangularis Linn, Cissus quadrangularis) is an evergreen perennial plant belonging to the genus Cissus, the buckthorn grape family. Sisus grows similar to ivy, and the generic name'Cissus' means'ivy' in Greek. It is native to Australia and New Zealand, and about 350 species are distributed worldwide. There are a variety of varieties in Sisus, such as Enrendanika, which has strong vitality called'grape ivy', and Antarctica, which is called'kangaroo ivy'. The stem is woody and has no tendrils, and the leaves are single-leaved (single leaves) with a length of 10cm, and have a leather-like feel and are thick. It is a common foliage plant because it breeds by cuttings and is easy to grow due to its strong vitality. Sisus Antarctica has ovate leaves, bright green, and irregularly serrated edges. Because it has tendrils, it hangs on other objects, so it can be attracted by connecting them with strings. Since it is sensitive to ozone, the degree of damage caused by ozone can be checked with the naked eye, so it can function as an ozone warning device when growing indoors.

Sisus, which lives in certain regions of Asia, Africa and the Arabian Peninsula, has long been used as a natural remedy to treat a variety of diseases.

In particular, Sisus balances the hormones related to obesity in our body. Among them, it is known to help weight loss by regulating leptin, an appetite suppressing hormone, and adiponectin, a fat synthesis inhibitory hormone. In addition, the antioxidants contained in Sisus are known to help reduce the risk of various cardiovascular diseases such as high blood pressure and arteriosclerosis by reducing the level of LDL cholesterol, which is known as bad cholesterol. In addition, it is known to increase serotonin excretion to help relieve stress, health of teeth and bones, reduce inflammation, improve respiratory disorders, prevent diabetes, improve immunity, and relieve menstrual pain.

Cisus, which has recently been in the spotlight as a diet material, is known to be excellent in decomposing carbohydrates, and quercetin and isoramneting components contained in Cisus are known to have excellent effects in decomposing fat, but with little side effects. Sisus is used overseas as a material for processed foods, medicinal foods, foods, etc. As it has been proven to inhibit fat absorption and fat synthesis and accumulation in fat cells, it is already used as a raw material for many diet products.

It is common to cultivate seeds without transplantation by sowing or cutting them at a time, and harvesting is possible only after cultivation for 1 year or more, 1 year and 2 months. Since it is a plant that grows in tropical and subtropical climates, it is common to cultivate it while maintaining 40 to 70% humidity at an average temperature of 21 to 25°C. At higher temperatures, active ingredients do not come out properly, and it does not grow well at temperatures below 21°C. Is known. In the conventional cissus cultivation method, pesticides are frequently sprayed in many areas, causing environmental pollution and there is a problem in that pesticides are accumulated in the produced cissus.

Korean Patent Registration No. 10-1286217 Korean Patent Registration No. 10-2161601 Korean Patent Publication No. 10-2019-0104388

An object of the present invention is to provide a method for cultivating a seedling that has a high content of active ingredients, which enables rapid harvesting, because the roots of Sisus are vigorous and healthy through seedling cultivation using a uniformly formulated soil composition. do.

In addition, since the soil composition fermented using soil microorganisms is used for transplantation and cultivation, it can prevent pests and pests and grow well without spraying or fertilizing pesticides that cause environmental pollution after planting.Therefore, the use of pesticides or fertilizers can be eliminated or greatly reduced. The purpose of this is to provide a safe and eco-friendly method of cultivation of cissus that can cultivate cissus even in areas where the use of pesticides or fertilizers is restricted.

In addition, it is an object of the present invention to provide a cultivation method capable of obtaining a cisace with a high content of active ingredients and growing well at high temperatures.

In the present invention in order to achieve the above object,

Preparing cissus seedlings;

A seedling transplant cultivation step of transplanting and cultivating the seedlings in a pot containing a soil composition for cultivation including volcanic soil, fermented humus, and fermented palm leaf;

A seedling transplanting cultivation step of cultivating the seedling transplanted cultivated cissus while transplanting one or more times in a larger pot containing the soil composition for planting the seedlings;

It provides a cultivation method of the seedlings including a soil cultivation step of planting and cultivating in the soil after the seedling transplanting cultivation step is finished.

The cultivation method, preferably

A seedling transplant cultivation step of transplanting a seedling seedling into a pot containing the soil composition for seedling cultivation and cultivating it for 20 to 30 days;

The first seedling transplant cultivation step of transplanting the seedling transplanted cultivated cissus into a larger pot containing the soil composition and cultivating it for 30 to 40 days;

A second seedling transplant cultivation step of transplanting the first transplanted cultivated cissus into a larger pot containing the soil composition and cultivating it for 30 to 40 days;

A third seedling transplant cultivation step of transplanting the second transplanted cultivated cissus into a larger pot containing the soil composition and cultivating it for 30 to 40 days; And

After the third seedling transplant cultivation step is over, it includes a soil cultivation step of cultivating it in the soil for at least 4 months.

The soil composition for cultivation of Sisus is preferably prepared by mixing volcanic soil, humus fermentation product, and palm leaf fermentation product in a weight ratio of 30-50:20-40:20-40, and fermenting for 70-120 days. More preferably, volcanic soil, fermented humus, and fermented palm leaf are mixed at a weight ratio of 35 to 45:25 to 35:25 to 35 and fermented for 90 to 100 days at a temperature of 35 to 39°C and a humidity of 30 to 40%. It was manufactured.

The fermented humus is preferably prepared by fermenting for 40 to 80 days while spraying liquid manure on humus or fallen leaves. More preferably, a liquid manure diluted 8 to 12 times on humus or fallen leaves is sprayed 22 to 28 times at regular time intervals at a weight ratio of 90 to 100:5, and 45 to 60 at a temperature of 35 to 39°C and a humidity of 60 to 80% It is made by fermenting for days.

The fermented palm leaf is preferably prepared by fermenting for 40 to 80 days while spraying liquid manure on the palm leaf. More preferably, the liquid ratio diluted 18 to 22 times in palm leaf is sprayed 22 to 28 times at regular time intervals at a weight ratio of 80 to 100:10, and for 45 to 60 days at a temperature of 35 to 39°C and a humidity of 60 to 80% It is made by fermentation.

The liquid manure is obtained by fermentation by inoculating an indigenous microbial culture solution containing Bacillus subtilis into a mixed by-product including a fish by-product and a sugar watermelon. More preferably, the liquid manure is indigenous containing Bacillus subtilis separated from humus in a mixed by-product comprising 20 to 30 parts by weight of fish by-product, 30 to 40 parts by weight of seawater, and 15 to 25 parts by weight of sugar cane Inoculating 8 to 12 parts by weight of the microorganism culture solution; First aging for 40 to 80 hours at 30 to 40°C after the inoculation; And separating only the liquid after the first aging, adding 8 to 12 parts by weight of rice bran powder, and secondary aging at 30 to 40°C for 40 to 80 hours.

In the cultivation method, the seedling transplanting cultivation step may be used by mixing a soil composition for cultivation of seeds and fallen leaves in a pot. When mixed and used, preferably, the soil composition for cultivation of Sisus and fallen leaves may be mixed in a weight ratio of 1:1 to 3:1.

In the method of cultivation of Sisius of the present invention, the seedlings of Sisius are grown while transplanting several times in a potted plant using a soil composition formed at a certain ratio, and then planted in the soil, so that the roots are remarkably healthy and the roots are remarkably healthy. , It is possible to harvest quickly due to its rapid growth, and in a short period of time, it is possible to obtain a sesace with a high content of active ingredients. In general, unlike the conventional method in which the seedlings can be harvested after 1 year or more after planting the seedlings, according to the cultivation method of the present invention, the seedlings can be harvested 4 months after planting in the soil. Harvesting is possible after 6 months, and stems can be harvested once every two months.

In addition, the cissus cultivation method of the present invention can cultivate cissus in a healthy way without separate pesticide spraying or fertilization after planting, and even in areas where the use of pesticides or fertilizers is prohibited, the cissus can be cultivated in an eco-friendly and safe manner without environmental pollution. I can.

In addition, according to the method of cultivating seeds of the present invention, seeds grow well at a high temperature of 28 to 33°C, which is higher than 21 to 25°C, which is a temperature at which the seeds are grown, and seeds with a high content of active ingredients are harvested. can do.

1 is a photograph of volcanic soil used in the present invention.
2 is a photograph of a process of fermenting by mixing humus and liquid manure.
3 is a photograph of a mixture of humus and liquid manure and fermentation for 3 weeks.
Figure 4 is a photograph of a process of fermentation by mixing palm leaf and liquid manure.
5 is a photograph of a mixture of volcanic soil, humus fermentation products, and palm leaf fermentation products.
6 is a photograph of seedlings to be cultivated.
7 is a photograph of a seedling of Sisus transplanted into a flower pot.
8 is a photograph of a seedling in which the seedlings transplanted into a flower pot were constantly grown.
9 is a photograph of Sisus during cultivation of primary seedlings.
10 is a photograph of a second seedling transplant cultivation of Sisus.
Fig. 11 is a photograph of a sequence before the third seedling is transplanted by growing consistently after the second seedling is transplanted.
12 is a photograph of a seedling cultivation in the third seedling transplantation.
13 is a photograph of Sisus 3 months after planting in the soil.
14 is a photograph of Sisus 4 months after planting in the soil.

The method of cultivation of the present invention,

Preparing cissus seedlings; A seedling transplant cultivation step of transplanting and cultivating a seedling seedling in a pot containing a soil composition for seedling cultivation; Seedling transplanting cultivation step of cultivating the seedlings while transplanting the seedlings at least once in a larger pot containing the soil composition for cultivation of Sisus; It includes a soil cultivation step of planting and cultivating in the soil after the seedling transplant cultivation step is completed.

Hereinafter, the method of cultivation of the present invention will be described in detail.

The soil composition for cultivation of Sisus used in the present invention includes volcanic soil, fermented humus and fermented palm leaf. The soil composition for cultivation of Sisus is preferably prepared by mixing volcanic soil, humus fermentation product, and palm leaf fermentation product at a weight ratio of 30 to 50:20 to 40:20 to 40 and fermenting for 70 to 120 days. More preferably, volcanic soil, fermented humus, and fermented palm leaf are mixed at a weight ratio of 35 to 45:25 to 35:25 to 35 and fermented at a temperature of 35 to 39°C and a humidity of 30 to 40% for 90 to 100 days. do. Particularly preferably, volcanic soil, fermented humus, and fermented palm leaf are mixed and fermented at a weight ratio of 40:30:30.

The volcanic soil is a soil produced using volcanic eruptions as a basic material, and'volcanic soil' in the present invention is defined as including a mixture of the volcanic soil and soil by a maximum of 2 times the weight of the volcanic soil.

The fermented humus is prepared by mixing and fermenting liquid manure with humus or fallen leaves. Preferably, it is prepared by fermenting for 40 to 80 days while spraying liquid manure on humus or fallen leaves. More preferably, a liquid manure diluted 8 to 12 times on humus or fallen leaves is sprayed 22 to 28 times at regular time intervals at a weight ratio of 90 to 100:5, and 45 to 60 days at a temperature of 35 to 39°C and a humidity of 60 to 80% It is prepared by fermenting during the period. In the present invention, humus (腐葉土) refers to the soil generated by decaying and decomposing leaves or small branches by microorganisms. The humus may be collected from nature or used commercially.

The fermented palm leaf is prepared by mixing and fermenting liquid manure in palm leaf. Preferably, it is prepared by fermenting for 40 to 80 days while spraying liquid manure on palm leaf. More preferably, a liquid manure diluted 18 to 22 times in palm leaf is sprayed 22 to 28 times at regular time intervals at a weight ratio of 80 to 100:10, and fermented for 45 to 60 days at a temperature of 35 to 39°C and a humidity of 60 to 80%. It is manufactured by ordering.

Liquid fertilizer (液肥) refers to a liquid fertilizer. Liquid manure can use a variety of commercialized products. Preferably, the liquid manure is obtained by fermenting a mixed by-product including a fish by-product and a sugarcane by using an indigenous microbial culture broth containing Bacillus subtilis.

More preferably, the liquid manure comprises the steps of inoculating an indigenous microbial culture solution to a mixed by-product including a fish by-product and a sugar cane; Primary aging after inoculation; And it is prepared by a method comprising the step of secondary aging by separating only the liquid after the first aging.

First, in a mixed by-product comprising 20 to 30 parts by weight of fish by-product, 30 to 40 parts by weight of seawater, and 15 to 25 parts by weight of sugar cane, 8 to 12 parts by weight of indigenous microbial culture solution containing Bacillus subtilis separated from humus Inoculate.

After the inoculation, the primary aging is performed at 30 to 40°C for 40 to 80 hours, preferably at 36 to 38°C for 48 to 60 hours.

After the first aging, only the liquid is separated and subjected to second aging to prepare a liquid manure. At this time, preferably, rice bran powder is added to the separated liquid, and secondary aging is performed at 30 to 40°C for 40 to 80 hours. More preferably, 8 to 12 parts by weight of rice bran powder is added to the separated liquid, followed by secondary aging at 36 to 38°C for 48 to 60 hours.

It is preferable to carry out the entire process of aging in the aging tank.

As the fish by-products, as well as by-products from the fish processing process, various fish species with low marketability can be used.

The liquid manure thus obtained is preferably diluted 8 to 12 times during fermentation of the humus, and most preferably diluted 10 times for use. In addition, during the fermentation of the palm leaf, it is preferable to use the obtained liquid ratio by dilution 18 to 22 times, and most preferably, it is used after diluting about 20 times. When diluting the liquid manure, it can generally be diluted with water.

The indigenous microorganism culture medium is obtained by separating and culturing a strain containing Bacillus subtilis from humus and soil.

When the seedlings are transplanted and cultivated several times in a potted plant using the soil composition for cultivation of the present invention as described above, and then planted in the soil, the soil composition creates an environment in which microorganisms can grow in the roots of the seeds. After planting, it is possible to greatly reduce pests and pests without using a separate pesticide, and roots are remarkably healthy and growth is accelerated due to active root growth, and the content of active ingredients is also increased.

The seedling cultivation method of the present invention using the Sisus cultivation soil composition, more preferably, a seedling transplant cultivation step of transplanting and cultivating a Sisus seedling in a pot containing the Sisus cultivation soil composition; The primary seedling transplant cultivation step of transplanting and cultivating the seedlings grown in the pots into larger pots containing the soil composition; A second seedling transplant cultivation step of transplanting and cultivating a larger potted plant containing the soil composition; A third seedling transplant cultivation step of transplanting and cultivating a larger potted plant containing the soil composition; It includes planting in the soil.

First, prepare the seedlings to be grown. Sisus seedlings are generally obtained by cutting seeds. It is preferable that the length of the seedling of the seed to be cultivated is 5 to 10 cm.

Sisus seedlings are transplanted into a first pot containing the soil composition for cissus cultivation, and are usually first cultivated for 20 to 30 days. Pots for primary cultivation are preferably pots with a diameter of 5 to 10 cm so as to be suitable for cultivation of Sisus seedlings.

When the seedlings transplanted into the pots grow constantly and become seedlings, the seedlings are first transplanted into the second potted plant containing the cissus cultivation soil composition and cultivated for 30 to 40 days. The second potted plant is larger than the first potted plant, and a potted plant having a diameter of 6 to 12 cm is preferable.

After the first seedling transplant cultivation, the seedlings are secondarily transplanted into the third potted plant containing the soil composition for seedling cultivation and cultivated for 30 to 40 days. The third potted plant is larger than the second potted plant, and a potted plant having a diameter of 10 to 15 cm is preferable.

After the second seedling is cultivated, the seedlings are thirdly transplanted into the fourth potted plant containing the soil composition for seedling and cultivated for 30 to 40 days. The fourth potted plant uses a large potted plant larger than the second potted plant, and a potted plant having a diameter of 15 to 20 cm is preferable.

In the seedling transplanting cultivation step, a soil composition for cultivation and fallen leaves may be mixed and used in a potted plant. Preferably, the soil composition and fallen leaves are mixed and used in the second and third seedling cultivation stages. When using a mixture of the soil composition for cultivation of Sisus and fallen leaves, it is preferable to mix and use the soil composition for cultivation of Sisus and the fallen leaves in a weight ratio of 1:1 to 3:1. More preferably, 1.5:1 to 2.5:1 are mixed and used.

After the seedlings are transplanted and cultivated, the seeds are planted in the soil, and the soil is cultivated for at least 4 months, preferably 4 to 6 months.

In the process of cultivating cissus as described above, it is preferable to irrigate twice a day before sunrise and after sunset.

The cultivation temperature can be cultivated at an average of 21~25℃, which is a general seasace cultivation temperature, as well as a higher average of 28~33℃. It is desirable to maintain the humidity at about 30 to 80%.

The seeds grown as described above can be planted in the soil and harvested after 4 months, and full-scale harvesting is possible after 6 months, and the stems can be harvested once every two months.

The method of cultivation of Sisus of the present invention is cultivated in the soil after a certain period of cultivation in the soil microbial environment and nutrient conditions optimized as described above. It is possible to cultivate cissus even at high temperatures of ~33℃.

The present invention will be described in more detail through the following examples. These examples are illustrative of the present invention and the scope of the present invention is not limited thereto.

<Example 1>

Preparation of soil composition for cultivation of Sisus

10 kg of indigenous microbial culture medium containing Bacillus subtilis isolated from humus was inoculated into a mixture of 30 kg of fish by-products, 30 kg of seawater and 20 kg of sugar cane and inoculated in a maturation tank at 36~38℃ for 52 hours 1 Let the tea mature. After the first aging, only the liquid was extracted, 10 kg of rice bran powder was added, followed by secondary aging at 36-38° C. for 52 hours to prepare a liquid manure.

Prepared volcanic soil. A photograph of the volcanic soil used in the present invention is shown in FIG. 1.

Beeleaf soil (manufactured by SAMUDERA) 5kg of liquid manure diluted 10 times in 95kg was sprayed 25 times at intervals of 48 hours and fermented for 50 days at 35~39℃ and 60~80% humidity to prepare a fermented humus. The process of fermenting by mixing humus and liquid manure is shown in FIGS. 2 and 3.

10 kg of liquid manure diluted 20 times to 90 kg of palm leaf was sprayed 25 times at intervals of 48 hours and fermented for 50 days at a temperature of 35 to 39° C. and a humidity of 60 to 80% to prepare a fermented palm leaf. Fig. 4 shows a process of fermenting by mixing palm leaf and liquid manure.

Fermented humus 90kg, fermented palm leaf 90kg and volcanic soil 120kg were mixed and fermented at 35~39℃ and 30~40% humidity for 95 days to prepare a soil composition for cultivation of Sisus. Fig. 5 shows a state in which volcanic soil, humus fermentation product, and palm leaf fermentation product are mixed.

<Example 2>

Preparation of soil composition for cultivation of Sisus

A soil composition for cissus cultivation was prepared in the same manner as in Example 1, except that the liquid manure purchased from Ori-Ocean Blue was used as the liquid manure. Volcanic Soil,

<Example 3>

Preparation of soil composition for cultivation of Sisus

A soil composition for Sisus cultivation was prepared in the same manner as in Example 1, except that humus purchased from Ori-Ocean Blue was used as humus.

<Example 4>

Cultivation of Sisus

Sisus seedlings were prepared for cultivation by cutting seeds. Fig. 6 shows a picture of seedlings to be cultivated.

The soil composition for cultivation of Sisus prepared in Example 1 was used for cultivation of Sisus.

Sisus seedlings were transplanted and cultivated for 30 days , containing the soil composition for cultivation of Sisus in a small first pot. 7 is a photograph of a seedling of Sisus transplanted into a potted plant, and FIG. 8 is a photograph of a seedling in which the seedlings are constantly grown.

After seedling transplant cultivation, the seedlings grown regularly were first transplanted into a larger second pot (medium pot) containing the soil composition for seedling cultivation and cultivated for 30 days (first seedling transplant cultivation). 9 is a photograph of Sisus during cultivation of primary seedlings.

Primary seedling transplantation The cultivated cissus was secondarily transplanted into a larger third pot (large pot) and cultivated for 30 days (second seedling transplant cultivation). At this time, the soil composition for cultivation of Sisus and fallen leaves were mixed in a ratio of 2:1 in the pot. FIG. 10 is a photograph of a second seedling cultivation during cultivation, and FIG. 11 is a photograph before a third seedling is transplanted after being constantly grown.

The second seedling transplanted and grown as shown in FIG. 11 was transplanted into the fourth pot, the largest large potted plant, and cultivated for 40 days (third seedling transplant cultivation). At this time, in a potted plant, the soil composition for cultivation of Sisus and fallen leaves were mixed in a 1:1 ratio. 12 is a photograph of a seedling cultivation in the third seedling transplantation.

When the tertiary seedling transplanted cultivated seedlings were grown as shown in FIG. 12, they were planted in the soil. 13 is a photograph of Sisus 3 months after planting. 14 is a photograph of a sisus that can be harvested 4 months after planting.

During the cultivation process of the cissus, watering was performed twice daily, before sunrise and after sunset.

<Experimental Example 1>

The components of the fermented humus fermented with liquid manure in Example 1 were analyzed, and the results are shown in Table 1 below.

Test Items unit result Method or equipment used Nitrogen (as N) (As is basis) %w/w 0.8 MS417:1994 Nitrogen (as N)(Dry basis) %w/w 6.3 MS417:1994 Phosphorus(as P ₂ O ₅ )(As is basis) %w/w 0.3 MS417:1994 Phosphorus (as P ₂ O ₅ )(Dry basis) %w/w 2.4 MS417:1994 Potassium (as K ₂ O) (As is basis) %w/w 0.3 MS417:1994 Potassium (as K ₂ O) (Dry basis) %w/w 2.4 MS417:1994 Humic acid (As is basis) %w/w 0.6 in House Method Humic acid (Dry basis) %w/w 4.7 in House Method Boron (as B ₂ O ₃ )(As is basis) mg/kg 182.0 AOAC 982.01, 17th Ed Boron (as B ₂ O ₃ )(Dry basis) mg/kg 0.1 AOAC 982.01, 17th Ed Magnesium (as MgO) (As is basis) %w/w 678.2 MS417:1994 Magnesium (as MgO)(Dry basis) %w/w 0.5 MS417:1994 Copper (as is basis) mg/kg 8.8 AOAC 965.09, 17th Ed Copper (as Cu) (Dry basis) mg/kg 69.3 AOAC 965.09, 17th Ed Zinc (as Zn) (As is basis) mg/kg 70.9 AOAC 965.09, 17th Ed Zinc (as Zn) (Dry basis) mg/kg 558.0 AOAC 965.09, 17th Ed moisture %w/w 87.3 oven(2 hours, 105℃)

<Experimental Example 2>

The components of the fish by-products used in Example 1 were analyzed and the results are shown in Table 2 below.

Test Items unit result Method or equipment used Nitrogen (as N)(Dry basis) %w/w 7.1 MS417:1994 Phosphorus (as P ₂ O ₅ )(Dry basis) %w/w 2.7 MS417:1994 Potassium (as K ₂ O) (Dry basis) %w/w 8.9 MS417:1994 moisture %w/w 88.8 oven(2 hours, 105℃)

<Experimental Example 3>

The components of the liquid manure prepared in Example 1 were analyzed and the results are shown in Table 3 below.

Test Items unit result Method or equipment used Nitrogen (as N)(Dry basis) %w/w 10.9 MS417:1994 Phosphorus (as P ₂ O ₅ )(Dry basis) %w/w 1.6 MS417:1994 Potassium (as K ₂ O) (Dry basis) %w/w 15.6 MS417:1994 moisture %w/w 93.6 oven(2 hours, 105℃)

<Experimental Example 4>

The components of the liquid manure used in Example 2 (Ori-Ocean Blue) were analyzed, and the results are shown in Table 4 below.

Test Items unit result Method or sheet used Nitrogen (as N) (As is basis) %w/w 13.6 MS417:1994 Phosphorus(as P ₂ O ₅ )(As is basis) %w/w 4.5 MS417:1994 Potassium (as K ₂ O) (Dry basis) %w/w 4.5 MS417:1994 Humic acid (Dry basis) %w/w 36.4 in House Method Boron (as B2O3)(Dry basis) mg/kg 679.5 AOAC 982.01, 17th Ed Magnesium (as MgO)(Dry basis) %w/w 1.1 MS417:1994 Copper (as Cu) (Dry basis) mg/kg ND<0.1 AOAC 965.09, 17th Ed Zinc (as Zn)(Dry basis) mg/kg 193.2 AOAC 965.09, 17th Ed moisture %w/w 95.6 oven(2 hours, 105℃) Arginine %w/w 2.34 AOAC 994.12&JAOAC,Vol71,No6,1988 Aspartic acid %w/w 0.00 AOAC 994.12&JAOAC,Vol71,No6,1988 Alanine %w/w 0.00 AOAC 994.12&JAOAC,Vol71,No6,1988 Glutamic acid %w/w 0.00 AOAC 994.12&JAOAC,Vol71,No6,1988 Glycine %w/w 0.12 AOAC 994.12&JAOAC,Vol71,No6,1988 Histidine %w/w 0.00 AOAC 994.12&JAOAC,Vol71,No6,1988 Isoleucine %w/w 0.12 AOAC 994.12&JAOAC,Vol71,No6,1988 Leucine %w/w 0.15 AOAC 994.12&JAOAC,Vol71,No6,1988 Lysine %w/w 0.11 AOAC 994.12&JAOAC,Vol71,No6,1988 Metaionine %w/w 0.07 AOAC 994.12&JAOAC,Vol71,No6,1988 Phenylalanine %w/w 0.00 AOAC 994.12&JAOAC,Vol71,No6,1988 Proline %w/w 0.00 AOAC 994.12&JAOAC,Vol71,No6,1988 Serine %w/w 0.04 AOAC 994.12&JAOAC,Vol71,No6,1988 Tyrosine %w/w 0.17 AOAC 994.12&JAOAC,Vol71,No6,1988 Threonine %w/w 0.35 AOAC 994.12&JAOAC,Vol71,No6,1988 Valine %w/w 0.06 AOAC 994.12&JAOAC,Vol71,No6,1988

<Experimental Example 5>

The components of the humus (manufactured by SAMUDERA) used in Example 1 were analyzed, and the results are shown in Table 5 below.

Test Items unit result Method or equipment used Nitrogen (as N)(Dry basis) %w/w 0.72 KJELDAHL Phosphorus (as P ₂ O ₅ )(Dry basis) %w/w 0.57 COLORIMETERIC Potassium (as K ₂ O) (Dry basis) %w/w 0.005 AAS Boron(B)(Dry basis) %w/w 0.007 AAS Magnesium (as MgO)(Dry basis) %w/w 0.12 AAS

<Experimental Example 6>

The components of the humus (manufactured by Ori-Ocean Blue) used in Example 3 were analyzed, and the results are shown in Table 6 below.

Test Items unit result Method or equipment used Nitrogen (as N)(Dry basis) %w/w 0.60 KJELDAHL Phosphorus (as P ₂ O ₅ )(Dry basis) %w/w 0.31 COLORIMETERIC Potassium (as K ₂ O) (Dry basis) %w/w 0.003 AAS Boron(B)(Dry basis) %w/w 0.012 AAS Magnesium (as MgO)(Dry basis) %w/w 0.044 AAS

Claims (8)

Preparing cissus seedlings;
A seedling transplant cultivation step of transplanting and cultivating the seedlings in a pot containing a soil composition for cultivation including volcanic soil, fermented humus, and fermented palm leaf;
A seedling transplanting cultivation step of cultivating the seedling transplanted cultivated cissus while transplanting one or more times in a larger potted plant containing the soil composition for planting the seedling;
After the seedling transplanting cultivation step is over, a method of cultivating a seedling comprising a soil cultivation step of cultivating it in the soil. The method of claim 1,
A seedling transplant cultivation step of transplanting a seedling seedling into a pot containing the soil composition for seedling cultivation and cultivating it for 20 to 30 days;
The first seedling transplant cultivation step of transplanting the seedling transplanted cultivated cissus into a larger pot containing the soil composition and cultivating it for 30 to 40 days;
A second seedling transplant cultivation step of transplanting the first transplanted cultivated cissus into a larger pot containing the soil composition and cultivating it for 30 to 40 days;
A third seedling transplant cultivation step of transplanting the second transplanted cultivated cissus into a larger pot containing the soil composition and cultivating it for 30 to 40 days; And
The cultivation method of Sisus, comprising a soil cultivation step of cultivating it in the soil for at least 4 months after the tertiary seedling cultivation cultivation step is finished. The method of claim 1,
The soil composition for cultivation of Sisus is cultivation of Sisus, characterized in that it is prepared by mixing volcanic soil, humus fermentation and palm leaf fermentation at a weight ratio of 30-50:20-40:20-40, and fermenting for 70-120 days. Way. The method of claim 2,
The soil composition for cultivation of Sisus is a mixture of volcanic soil, humus fermentation product, and palm leaf fermentation product at a weight ratio of 35 to 45:25 to 35:25 to 35, and at a temperature of 35 to 39°C and a humidity of 30 to 40% for 90 to 100 days. A method of cultivation of Sisus, characterized in that it is produced by fermentation. The method according to any one of claims 1 to 4,
The fermented humus is prepared by fermenting for 40 to 80 days while spraying liquid manure on humus or fallen leaves,
The fermented palm leaf is prepared by fermenting for 40 to 80 days while spraying liquid manure on the palm leaf,
The liquid manure is obtained by fermenting an indigenous microbial culture solution containing Bacillus subtilis in a mixed by-product including a fish by-product and a sugar watermelon. The method of claim 5,
The liquid manure is,
Inoculating 8 to 12 parts by weight of indigenous microbial culture solution containing Bacillus subtilis isolated from humus into mixed by-products including 20 to 30 parts by weight of fish by-product, 30 to 40 parts by weight of seawater and 15 to 25 parts by weight of sugar cane. step;
First aging for 40 to 80 hours at 30 to 40°C after the inoculation; And
The cultivation method of Sisus, characterized in that it is prepared by a method comprising the step of separating only the liquid after the first aging, adding 8 to 12 parts by weight of rice bran powder, and secondary aging at 30 to 40°C for 40 to 80 hours. The method of claim 6,
The humus fermentation product is sprayed 22 to 28 times at regular time intervals at a weight ratio of 90 to 100:5 with 8 to 12 times diluted liquid manure on humus or fallen leaves, and 45 to 60 at a temperature of 35 to 39°C and a humidity of 60 to 80%. It is made by fermenting for one day,
The fermented palm leaves are sprayed 22 to 28 times at regular time intervals at a weight ratio of 80 to 100:10 with a liquid manure diluted 18 to 22 times in palm leaves, and for 45 to 60 days at a temperature of 35 to 39 °C and a humidity of 60 to 80% A method of cultivation of Sisus, characterized in that it is produced by fermentation. The method according to any one of claims 1 to 4,
The seedling transplanting cultivation step is a method of cultivation of Sisus, characterized in that the soil composition for cultivation of Sisus and fallen leaves are mixed and contained in a weight ratio of 1:1 to 3:1 in a pot.

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