KR101882678B1 - Pesticidal composition for controlling mushroom fly comprising Tanacetum cinerariifolium extracts and effective enhancer as active ingredient - Google Patents

Abstract

The present invention relates to an insecticidal composition for controlling mushroom flies containing an extract of Dalmatian insecticide and an effect enhancer as an active ingredient. More particularly, it relates to a insecticidal composition for controlling mushroom flies when Dalmatian insecticide extract, effect enhancer and smoke- It has a high insecticidal power against adults and utilizes eco-friendly materials. It can be usefully and safely used in agriculture.

Description

Technical Field [0001] The present invention relates to a pesticidal composition for controlling mushroom flies, which comprises Dalmatian bug insecticide extract and an effective enhancing agent,

The present invention relates to an insecticidal composition for controlling mushroom flies comprising Dalmatian bugs extract and an effect-enhancing agent.

The most severely damaging insects in domestic mushroom cultivation are mushroom flies, which are one of the major causes of mushroom production in the mushroom growing season. The mushroom fly larvae cut mushroom by cutting off mycelium and cutting it into mushroom fruiting body while directly impregnating the mushroom badge, and leaving a trail to reduce the merchantability and mediating pathogens such as various bacteria and blue fungi on the body, . In addition, mushroom fly adults carry worms that grow mushrooms, contaminate the fungi with pathogenic bacteria or fungi, and mediate mushroom mites and nematodes, which is an important cause of decrease in yield and deterioration of quality due to secondary damage.

However, the registration drug for controlling mushroom flies is only two kinds of diflubenzuron (teflubenzuron) and teflubenzuron (mushroom) that inhibit the formation of chitin in the epidermis of the trichomes and inhibit the peeling of the mushroom flies. In the case of mushroom mushrooms, And it is difficult to control because there are many restrictions on the use of medicines due to the residual problem of pesticides. In addition, since the registered medicines are only for the insecticidal insecticides of mosquitoes, they have been used for a long time even though they are sprayed alternately at the cultivation farmers. Furthermore, the use and abuse of organic synthetic pesticides for agricultural pest control measures indispensable to crop production is a major concern for the environment, such as the appearance of resistant pests, soil and food residues, toxicity to manure, It can cause side effects of disturbance.

Therefore, development of environmentally friendly natural pesticides that can replace organic synthetic pesticides and have excellent effects is required.

1. Korean Patent No. 10-0537946

1. Research institute Seoul National University, Plant origin of low toxicity selective toxicity Development of mushroom flies control agent, final report of the Ministry of Agriculture (2006.07.10).

It is an object of the present invention to provide an insecticidal composition for controlling mushroom flies, which comprises an extract of Danacetum cinerariifolium and an effect enhancer as an active ingredient, wherein the effect enhancer is selected from the group consisting of olive, grapefruit seed extract, ginkgo leaf extract, Wherein the insecticide composition is selected from the group consisting of insecticidal compositions for controlling mushroom flies.

It is another object of the present invention to provide a mushroom flies insecticidal method comprising the step of mixing and spraying the insecticidal composition for controlling mushroom flies, the effect promoting agent and the smoke filming agent, wherein the effect enhancer is selected from olive, grapefruit seed extract, Wherein the extract is selected from the group consisting of extracts, extracts of Parsley, and extracts of Pisum sativum.

In order to achieve the above object, the present invention provides an insecticidal composition for controlling mushroom flies, comprising an extract of Danacetum cinerariifolium and an effect enhancer as an active ingredient, wherein the effect enhancer is selected from olive, grapefruit seed extract, ginkgo leaf extract, Wherein the extract is selected from the group consisting of Parsley extract and Pseudomonas aeruginosa extract. The insecticidal composition for controlling mushroom flies is characterized in that the Dalmatian insect repellent extract and the effect enhancer are contained in a weight ratio of 1: 1.

In one embodiment of the present invention, the Dalmatian insect repellent extract, the dil seed extract, the parsley extract, or the sesame extract may be dissolved in water, methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene Chloride, hexane, and cyclohexane. The solvent extract may be one or more selected from the group consisting of hydrochloride, chloride, hexane, and cyclohexane.

In one embodiment of the present invention, the effect enhancer may be one that increases the insecticidal activity against the mushroom flies of Dalmatian bug extract.

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In one embodiment of the invention, the mushroom flies are selected from the group consisting of Camptomyia spp. Or Lycoriella mushroom flies ingenua .

In one embodiment of the present invention, the mushroom flies may be adults or larvae.

Also, the present invention provides a mushroom flies insecticidal method comprising the step of spraying a mixture of the insecticide composition for controlling mushroom flies, an effect enhancer and a smoke film agent, wherein the effect enhancer is selected from the group consisting of olive, grapefruit seed extract, Wherein the Dalmatian insect repellent extract and the effect enhancer are contained in a weight ratio of 1: 1.

In one embodiment of the present invention, the plasticizer may include glycerin and water.

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In one embodiment of the present invention, the plasticizer may be glycerin and water in a weight ratio of 1: 0.1 to 1:10, preferably 1: 2 to 1: 5, more preferably 1: : 3, but is not limited thereto. In addition, the dilution ratio of glycerin and water can be changed depending on the size and environmental conditions of the mushroom grower.

In one embodiment of the present invention, the spraying may be carried out in a hot fog manner.

In one embodiment of the invention, the mushroom flies are selected from the group consisting of Camptomyia spp. Or Lycoriella mushroom flies ingenua .

In one embodiment of the present invention, the mushroom flies may be adults or larvae.

The Dalmatian pest control extract according to the present invention is an eco-friendly material and has a high insecticidal activity against mushroom flies. When the efficacy enhancer is mixed with the Dalmatian pest control extract, the insecticidal power of the Dalmatian pest is increased and the Dalmatian pest control extract, When the mixture is sprayed by heating and fogging method, the insecticidal effect of the mushroom flies is remarkable, which can be usefully and safely used in agriculture.

Fig. 1 shows mushroom mushroom which was damaged by mushroom fly larvae.
Figure 2 shows the mushroom fly larvae that are damaging the mushroom mushroom.
Figure 3 shows mushroom fly adults.
Fig. 4 is a graph showing the insecticidal effect against the mushroom fly adult when diluted Dalmatian bug extract, diluted plant extract, and smoke film were sprayed by the heat mist method. Untreated: only water sprayed by heating and fogging; Contrast: Smoothening agent and Dalmatian bug bug extract were sprayed with 50 times dilution and heated and sprayed; Olive 6 times: Smoothening agent, a 50-fold dilution of dalmatian bug extract and a 6-fold dilution of olive were sprayed with heated mist; GSE 6 fold: Smoothening agent, a 50-fold dilution of Dalmatian pest control extract and a 6-fold dilution of grapefruit seed extract were sprayed by heating and fogging; Bank 6 times: group of smoke, diluted 50 times of dalmatian bug extract and 6 times of diluted ginkgo biloba extract with heating and fogging; Diss Seed Triplicate: Smoothening agent, a 50-fold dilution of Dalmatian trefoil extract and a 3-fold dilution of the dil seed extract were sprayed by heating and fogging; Parsley triple: Smoothening agent, a 50-fold dilution of Dalmatian pest control extract and a 3-fold dilution of parsley extract by heating and spraying; And three times as much as the diluent: smoke, 50-fold dilution of Dalmatian bug extract and 3-fold dilution of Aspergillus sp.

The present invention provides an insecticidal composition for controlling mushroom flies comprising Dalmatian bug extract extract and an effect-enhancing agent as an active ingredient.

The term " effect-enhancing agent " of the present invention refers to a substance that increases the insecticidal effect or insecticidal power. In the present invention, it refers to increasing the insecticidal power of the mushroom fly against the Dalmatian bug extract.

The controlling insecticidal composition of the present invention may further comprise an agriculturally acceptable carrier.

The carrier may be inorganic or organic and of synthetic or natural origin, and the active composition may be mixed with or formulated with the carrier to facilitate the storage, movement and / or handling of the plant, seed, soil or other treated subject, Of the substance. In general, any material that can be routinely used as a carrier in pesticides, herbicides or antimicrobial compositions is suitable for use in the present invention. The insecticidal compositions for the control of natural mushroom flies of the present invention may be used alone or in combination with the solid and / or liquid dispersible carrier vehicles and / or other compatible compatible actives, in particular other insecticides, In the form of a mixture with a plant protection agent such as a herbicide, a nematicide, a fungicide, a bactericide, a rodent remover, a herbicide, a fertilizer, a growth regulator, etc., or, if desired, For example, they may be used in ready-to-use forms such as solutions, emulsions, suspensions, powders, pastes and granules. The pesticidal composition for controlling natural mushroom flies of the present invention may optionally contain conventional inert (plant compatible or herbally inert) insecticide diluents or swelling agents of the type available for conventional insecticidal agents or compositions, for example, The present invention also relates to a method for producing a microcapsule comprising the steps of mixing a microcapsule, a microcapsule, a microcapsule, a microcapsule, a microcapsule, a microcapsule, a suppositories, A coating composition for use in seeds, and a preparation for use in combination with burning equipment such as a foaming cartridge, foamable can and foaming coils, as well as ULV cold air mist and hot air fog formulation. The carrier is preferably adjusted according to the formulation, spraying location and spraying method of the insecticide composition for controlling natural mushroom flies.

The insecticidal composition for controlling mushroom flies can be diluted to an appropriate concentration at the time of use.

As the diluting solvent, at least one selected from the group consisting of water and a surfactant can be used, and for example, water can be used.

The present invention provides a mushroom flue control product (e.g., an insecticide) comprising the above composition.

The mushroom flameproofing product may be formulated into a suitable form depending on the purpose and use of the mushroom. For example, the mushroom flies can be granules, powders, solutions, aerosols, spray agents, exfoliants, pastes, The composition may be a capsule, a liquid wettable powder, a granule wettable powder, a wettable powder, a powder, a fine granule, an oil, a gel broth, a fuming agent, a smoke film, a fumigant and the like. Fumigants or aerosols are most preferred.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

Example  One. Dalmatian bug bug  Manufacturing process of extract

Dried flowers of Dalmatian bug bug were dried for 5 ~ 6 hours at 50 ℃ to expose the harvested Dalmatian bug bug to sunlight for 5 hours and then to prevent breakdown of pyrethrin by using a hot air dryer. Then, 100 g of the dried flower of Dalmatian trephinist was put into a 500 ml fermentation alcohol (95% ethanol) and pulverized and immersed for 3 weeks to obtain an extractant solution of 2,400 ppm. The extract stock solution obtained was diluted 50 times (48 ppm) and used.

Example  2. Manufacturing process of effect enhancer

In order to improve the insecticidal effect, various plants were used for the experiment. The effect-enhancing agent in the present invention refers to olive, grapefruit seed extract, gingko leaf extract, dill seed extract, parsley extract or sesame extract.

 First, olives were used in commercially available olive oil of extra virgin olive oil from Italy, diluted 6 times with water and mixed with 1: 1 diluted with 50 times diluted solution of Dalmatian bugs extract.

Grapefruit Seed Extract (GSE) is a commercially available edible grapefruit seed extract (DF-100, glycerin 50% and naringin 0.48%) diluted 6-fold with water and mixed with a 50-fold dilution of dalmatian bug extract Respectively.

The bank was diluted 6 fold with water and diluted 1: 1 with a 50-fold diluted solution of Dalmatian bug extract. The extracts of Ginkgo biloba leaf extract (Garden keeper, Organic agricultural material disclosure -1-6-019, Ginkgo biloba extract 54.4%, Surfactant 44.3%, Citric acid 1.3% 1.

Dill seeds were prepared by diluting 400 g of dry dill seed (ShinYoung FS, imported) with 3 times dilution of dill seed extract which was pulverized and immersed in 1.2 L fermented alcohol (95%) for 3 weeks and diluted with 50 times diluted solution of dalmatian bug extract 1: 1.

Parsley was purchased by purchasing Parsley Seed (Aram Seedling) and 500g of parsley leaves and stems grown in a plastic house in Sindeokri vinyl house in Cheonan, Gwangdeok-myeon were pulverized for 3 weeks in 1.0L fermented alcoholic beverages (95%) and diluted 3 times with water The mixture was mixed with a 50-fold diluted solution of Dalmatian bug extract at a ratio of 1: 1.

(100%), which was cultivated in Sindeokri vinyl house in Cheonan, Gwangdeok-myeon, was purchased for 2 weeks from the date of fermentation (95%) for 3 weeks. Diluted 3 times with water and 1: 1 with a 50-fold dilution of Dalmatian bug extract.

Example  3. Dalmatian bug bug  Methods of spraying extracts, effect-enhancing agents and smoke-

A 50-fold dilution of the dalmatian bug extract extract and the effect-enhancing agent of Example 2 above were mixed with the surfactant. The mixture of glycerin and water was used as a softener. In the experiment, a small size softener was used. Therefore, glycerin and water were mixed at a weight ratio of 1: 3. The extract and the smoke mix were sprayed on the mushroom flies using a hot smoke sprayer using a small softener. The experiment was conducted by irradiating 100 mushroom flies collected in a mushroom flies cultivar (Myeongseongseon-myeon) in a 50 * 50 * 50 cm transparent acrylic breeding box. After 30 minutes of spraying the mixture of the extract and the smoke mixture with heating and fogging method, the insecticidal activity against the dalmatian bug bug and the effect-enhancing agent was analyzed through the mortality of the mushroom flies.

Example  4. Dalmatian bug bug  Extract, effect-enhancing agent and smoke Insecticide  analysis

After diluting Dalmatian bug bug extract and effect enhancer diluted with smoke, the survival and movement of mushroom flies were observed after 30 minutes. The control group was sprayed with water only and the control group was sprayed with a diluent of 50 times diluted solution of Dalmatian pest control solution and a surfactant.

As a result, the mortality rate of mushroom flies was 0% in the case of water spraying, and the individuals ascending to the acrylic box wall and the moving objects on the floor were observed. In the case of spraying 50 times diluted solution (48 ppm) of smoke and dalmatian bug extract without addition of effect-enhancing agent, the mortality rate of mushroom flies was about 31% compared to untreated group, Dull individuals were observed (Fig. 4).

In addition, when the 50% diluted solution (48 ppm) of Dalmatian bug bug and the 3 times diluted solution of dil seed were sprayed together with the surfactant, the mortality rate of mushroom flies was 85% as compared to the untreated group. In the case of 6-fold dilution of GSE (Grapefruit seed extract) and 3-fold dilution of shrimp, the mortality of mushroom flies was 73-74% compared to the untreated group, and the 6-fold dilution of olive and parsley Compared with the untreated group, the mortality rate of the mushroom flies was about 50%, and the 6-fold dilution of the bank showed about 23% lower insecticidal activity than the control group ).

Therefore, it was confirmed that the insecticidal power against the mushroom flies was increased when the Dalmatian bug bug extract and the effect enhancer were sprayed. As a result, Dalmatian trephine extract and effect enhancer can be utilized in various fields in agriculture as an environmentally friendly control means.

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Claims (13)

An insecticidal composition for controlling mushroom flies comprising an extract of Tanacetum cinerariifolium and an effect-enhancing agent as an active ingredient,
Wherein the effect enhancer is any one selected from the group consisting of olive, grapefruit seed extract, dill seed extract, parsley extract and sesame extract, and the Dalmatian insect repellent extract and the effect enhancer are contained in a weight ratio of 1: 1 Insecticidal composition for controlling mushroom flies. The method according to claim 1,
The Dalmatian insecticide extract, the lecithin extract, the parsley extract or the sesame extract may be dissolved in water, methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane ≪ / RTI > or a pharmaceutically acceptable salt thereof. The method according to claim 1,
Wherein the effect enhancer increases insecticidal activity against the mushroom flies of Dalmatian bug extract. delete The method according to claim 1,
The mushroom flies were found in mushroom or mosquitoes ( Camptomyia spp . ) Or a long beard mushroom fly ( Lycoriella ingenua ). The method according to claim 1,
Wherein the mushroom flies are adult or larvae. A mushroom flora insecticidal method comprising the step of mixing and spraying Dalmatian bug insecticide extract, effect enhancer and smoke film agent of claim 1,
Wherein the effect enhancer is any one selected from the group consisting of olive, grapefruit seed extract, dill seed extract, parsley extract, and sesame extract, and the Dalmatian insect repellent extract and the effect enhancer are contained in a weight ratio of 1: 1 Mushroom flies insecticide method. delete 8. The method of claim 7,
Wherein the plasticizer comprises glycerin and water, and the plasticizer comprises glycerin and water in a weight ratio of 1: 0.1 to 1:10. delete 8. The method of claim 7,
Characterized in that the spraying is carried out in a heated fog manner. 8. The method of claim 7,
The mushroom flies were found in mushroom or mosquitoes ( Camptomyia spp . ) Or a long beard mushroom fly ( Lycoriella ingenua ). 8. The method of claim 7,
Wherein said mushroom fly is an adult or larva.

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