CN109362814A - A kind of biological insecticides and preparation method thereof that preventing and treating orchard insect pest and application method - Google Patents
A kind of biological insecticides and preparation method thereof that preventing and treating orchard insect pest and application method Download PDFInfo
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- CN109362814A CN109362814A CN201811507008.1A CN201811507008A CN109362814A CN 109362814 A CN109362814 A CN 109362814A CN 201811507008 A CN201811507008 A CN 201811507008A CN 109362814 A CN109362814 A CN 109362814A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/32—Ranunculaceae [Buttercup family], e.g. hepatica, hydrastis or goldenseal
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/46—Stemonaceae [Stemona family], e.g. croomia
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- Biotechnology (AREA)
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- Wood Science & Technology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
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Abstract
The invention discloses a kind of biological insecticides for preventing and treating orchard insect pest and preparation method thereof and application methods.Biological insecticides by being made following preparation method: it takes tuber of stemona grass block root slice to decoct, tuber of stemona grassland liquid is obtained by filtration, tuber of stemona grassland liquid is diluted with water, the tuber of stemona grassland liquid after being diluted；Beauveria bassiana is inoculated with, is cultivated, obtain beauveria bassiana culture solution, slurry paste ground into juice after the cutting of tuber of stemona grass blade leaf is mixed well, anaerobic fermentation is sealed jointly in the fermenter with beauveria bassiana culture solution, by the product filtering after fermentation, fermentation liquid is obtained, fermentation filter residue is obtained after filtering；Fermenation raw liquid is diluted with water, the fermenation raw liquid after being diluted.Application method is that 1:1 is mixed by volume with the fermenation raw liquid after dilution by the tuber of stemona grass block stoste after above-mentioned dilution；Fruit tree is sprayed or brushed after mixing, or original hole of trunks of fruit trees is irrigated or is irrigated by Manual punching, or is mixed again after mixing with fermentation filter residue, soil, and the hole on trunks of fruit trees block or brush to trunks of fruit trees.
Description
Technical field
The present invention relates to a kind of biological insecticides for preventing and treating orchard insect pest.In addition, the invention further relates to a kind of prevention and treatment orchards
The process for preparing bio-insecticide of insect pest.In addition, making the invention further relates to a kind of biological insecticides for preventing and treating orchard insect pest
Use method.
Background technique
Longicorn is the general name of Polyphaga orchard section insect, is main on production of forestry, arable farming and construction timber
One of pest, and the primary pest for endangering orchard.Orchard is biting mouthparts, and palate is strong, gnaws that ability is strong, and adult gnaws
Newborn branch, bark, limb, young fruit, achievement can leave strip scar after gnawing.Serious, branch bark is gnawed light, is made
It is withered at fruiting branch frangibility.And adult can lay eggs at limb gap and in bark, and the larva after egg hatching can be along wooden
Portion, marrow eat into downwards food, up to root, can lead to that plant strain growth is bad, and tree vigo(u)r early ageing seriously affects achievement yield, when serious
Complete stool can be caused withered.Adult gnaws everywhere, and oviposition, larva moves in inside trees, is not easy to prevent and treat.Main prevention and treatment is done at present
Method is chemical prevention, although killing rate is big, control efficiency is good, is easy contaminated soil, air, fruit, chemical residual amount is big, shadow
Edible health is rung, while being easy that orchard is made to develop drug resistance.There is a method in which trap-tree need to be planted by being traped by trap-tree, but its
Heavy workload, effect are unobvious.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide one kind safely, effectively to kill fruit
Garden insect pest, without drug resistance, avoid environment by it is contaminated prevention and treatment orchard insect pest biological insecticides.
The present invention also provides a kind of process for preparing bio-insecticide for preventing and treating orchard insect pest.
The present invention also provides a kind of application methods of biological insecticides for preventing and treating orchard insect pest.
Technical solution used by the process for preparing bio-insecticide of prevention and treatment orchard insect pest of the invention is: including following
Step:
(1) tuber of stemona grass block root slice is taken to decoct, root tuber and water quality ratio press 1:25~1:40, and water is cooked by slow fire 60 after opening~
90min is obtained by filtration tuber of stemona grassland liquid, is cooled to room temperature；
(2) tuber of stemona grassland liquid and water 1:25~1:40 in mass ratio are diluted, the tuber of stemona grassland liquid after being diluted is standby
With；
(3) beauveria bassiana is inoculated with, cultivated, obtain beauveria bassiana culture solution, beauveria bassiana in culture solution
The concentration of spore is 100~120/ml；
(4) grinder slurry paste ground into juice will be used after the cutting of tuber of stemona grass blade leaf, mixed well, with beauveria bassiana culture solution
It is sealed anaerobic fermentation jointly in the fermenter, the mass ratio of cauline leaf starchiness juice and beauveria bassiana culture solution is 10:1
~15:1, the temperature of fermentation are 26 ± 2 DEG C, and the time of fermentation is 6~8 days；
(5) product after fermentation is filtered, obtains fermentation liquid, fermentation filter residue is obtained after filtering, it is spare；
(6) after according to volume ratio 1:25~1:40 of fermenation raw liquid and water dilution, the fermenation raw liquid after being diluted is standby
With.
The tuber of stemona grass is using radix stemonae tuberosae or Radix stemonae japonicae or radix stemonae sessilifoliae.
Technical solution used by the biological insecticides of prevention and treatment orchard insect pest of the invention is: by the insect pest of above-mentioned prevention and treatment orchard
Process for preparing bio-insecticide be made.
The first technical solution used by the application method of the biological insecticides of prevention and treatment orchard insect pest of the invention is: will
1:1 is mixed tuber of stemona grass block stoste after above-mentioned dilution by volume with the fermenation raw liquid after dilution；After mixing to fruit tree into
Row sprinkling is brushed.
Second of technical solution used by the application method of the biological insecticides of prevention and treatment orchard insect pest of the invention is: will
1:1 is mixed tuber of stemona grass block stoste after above-mentioned dilution by volume with the fermenation raw liquid after dilution；To fruit tree tree after mixing
Dry original hole is irrigated or is irrigated by Manual punching.
The third technical solution used by the application method of the biological insecticides of prevention and treatment orchard insect pest of the invention is: will
1:1 is mixed tuber of stemona grass block stoste after above-mentioned dilution by volume with the fermenation raw liquid after dilution；It is filtered after mixing with fermentation
Slag, soil mix again, and block to the hole on trunks of fruit trees.
4th kind of technical solution used by the application method of the biological insecticides of prevention and treatment orchard insect pest of the invention is: will
1:1 is mixed tuber of stemona grass block stoste after above-mentioned dilution by volume with the fermenation raw liquid after dilution；It is filtered after mixing with fermentation
Slag, soil mix again, and brush to trunks of fruit trees.
The beneficial effects of the present invention are: the biological insecticides of prevention and treatment orchard insect pest of the invention use beauveria bassiana culture
Liquid and tuber of stemona grass block root and cauline leaf are fermented as main function raw material using specific technique, filtrate and filter after fermentation
Slag is utilized effectively, and for preventing and treating the insect pest of peach garden orchard, beauveria bassiana is a kind of ascomycetous entomogenous fungi, white deadlock
The high cryptogam of bacterium is non-toxic and tasteless, non-environmental-pollution, has persistent infection power to pest, and pest can continuously disseminate propagation once infection,
It is residual without agriculture, any pesticide residue, non-resistant will not be caused harvesting product immediately after applying muscardine, muscardine passes through
Lethal with the contact infection of the body wall of pest under field conditions (factors), pest will not generate any resistance to it, use year after year, effect is anti-
And it is higher and higher, beauveria bassiana contains living body fungi and spore, after applying field, by suitable temperature, humidity, Ke Yiji
Continuous flourish enhances insecticidal effect, has regrowth, different from chemical pesticide do not distinguish between the enemy and ourselves by beneficial insect, pest total number
Poisoning, muscardine specificity is strong, smaller on the beneficial insects influence such as non-target organism such as ladybug, Chrysopa and food aphid horsefly, with Gao Xuan
Selecting property, therefore muscardine is a kind of environment friendly agricultural, ideal pesticide, not only can safely and effectively control orchard insect pest, but also can protect
The places such as phylactocarp garden avoid ecological environment from destroying by pollution；Tuber of stemona grass, Ranunculaceae, delphinium, away from ground leave plant, extensively
It is distributed in Central China the Yellow River, Yangtze river basin each province, it is mostly wild in height above sea level 500~2800 meters of mountainous regions grass slope or hills sandy ground,
Main product Shaanxi, Shandong, Anhui, Jiangsu, Zhejiang etc. save；It to be used as medicine with root tuber, root tuber contains a variety of alkaloids, take energy moistening lung to arrest cough orally,
External application can delousing desinsection, have itching-relieving action；When antibacterial action in vitro test, tuber of stemona decoction and radix stemonae tuberosae alcohol immersion liquid are to a variety of
Pathogenic bacteria such as pneumococcus, beta hemolysis type streptococcus, meningococcus, staphylococcus aureus, staphylococcus albus and dysentery
Shigella, typhoid bacillus, paratyphosum Bacterium, Escherichia coli, proteus, corynebacterium diphtheriae, pneumobacillus, yersinia pestis, anthrax
Bacillus, hay bacillus and comma bacillus, Bacillus tuberculosis etc. have different degrees of bacteriostasis, Radix stemonae japonicae water logging
Liquid has certain inhibiting effect to certain pathomycetes in vitro；It is practiced and is tested, the present invention reaches the eradicative rate in orchard
95% or more, be it is a kind of safely, effectively kill orchard, without drug resistance, avoid environment by contaminated prevention and treatment orchard insect pest
Biological insecticides.
Specific embodiment
The biological insecticides of prevention and treatment orchard insect pest of the invention by being made following preparation method, specifically includes the following steps:
(1) tuber of stemona grass block root slice is taken to decoct, root tuber and water quality ratio press 1:25~1:40, and water is cooked by slow fire 60 after opening~
90min is obtained by filtration tuber of stemona grassland liquid, is cooled to room temperature；
(2) tuber of stemona grassland liquid and water 1:25~1:40 in mass ratio are diluted, the tuber of stemona grassland liquid after being diluted is standby
With；
(3) beauveria bassiana is inoculated with, cultivated, obtain beauveria bassiana culture solution, beauveria bassiana in culture solution
The concentration of spore is 100~120/ml；
(4) grinder slurry paste ground into juice will be used after the cutting of tuber of stemona grass blade leaf, mixed well, with beauveria bassiana culture solution
It is sealed anaerobic fermentation jointly in the fermenter, the mass ratio of cauline leaf starchiness juice and beauveria bassiana culture solution is 10:1
~15:1, the temperature of fermentation are 26 ± 2 DEG C, and the time of fermentation is 6~8 days；
(5) product after fermentation is filtered, obtains fermentation liquid, fermentation filter residue is obtained after filtering, it is spare；
(6) after according to volume ratio 1:25~1:40 of fermenation raw liquid and water dilution, the fermenation raw liquid after being diluted is standby
With.
The tuber of stemona grass is using radix stemonae tuberosae or Radix stemonae japonicae or radix stemonae sessilifoliae, through testing, using the effect of radix stemonae tuberosae
More preferably.
The application method of the biological insecticides of prevention and treatment orchard insect pest of the invention is exemplified below:
(A) by the tuber of stemona grass block stoste after above-mentioned dilution, 1:1 is mixed by volume with the fermenation raw liquid after dilution；It is mixed
Fruit tree is sprayed after even；
(B) by the tuber of stemona grass block stoste after above-mentioned dilution, 1:1 is mixed by volume with the fermenation raw liquid after dilution；It is mixed
Fruit tree is brushed after even；
(C) by the tuber of stemona grass block stoste after above-mentioned dilution, 1:1 is mixed by volume with the fermenation raw liquid after dilution；It is mixed
Original hole of trunks of fruit trees is irrigated after even or is irrigated by Manual punching, if Manual punching, one tree should not be more
In 10 holes；
(D) by the tuber of stemona grass block stoste after above-mentioned dilution, 1:1 is mixed by volume with the fermenation raw liquid after dilution；It is mixed
It mixes again with fermentation filter residue, soil after even, and the hole on trunks of fruit trees is blocked；
(E) by the tuber of stemona grass block stoste after above-mentioned dilution, 1:1 is mixed by volume with the fermenation raw liquid after dilution；It is mixed
It mixes again with fermentation filter residue, soil after even, and trunks of fruit trees is brushed.
By the biological insecticides of the prevention and treatment orchard insect pest of above-described embodiment, in the village Jiashan of Zhejiang Xian Yao, whitefish swings 110 mu certainly
There is yellow peach garden to be implemented, specific implementation process are as follows:
(1) after orchard larva comes into play, (C) and (D) step in above-mentioned application method citing is carried out March；
(2) May, orchard start to lay eggs, and carry out (B) and (E) step in above-mentioned application method citing；
(3) July~August part carries out (A) and (C) step in above-mentioned application method citing.
Above procedure, every 5 days are a cycle, check medication effect, and repeat to be administered.
Above embodiments are only for the purpose of illustration to be used, and is not construed as limiting the scope of the present invention.It is not departing from
All using description of the invention and the various equivalence changes done in the case where inventive concept, reason is the same as being contained in the present invention
Scope of the claims in.
The biological insecticides of prevention and treatment orchard insect pest of the invention are using beauveria bassiana culture solution and tuber of stemona grass block root and stem
Leaf is fermented as main function raw material using specific technique, and filtrate and filter residue are utilized effectively after fermentation, is used
In prevention and treatment peach garden orchard insect pest, beauveria bassiana is a kind of ascomycetous entomogenous fungi, and the high cryptogam of muscardine is non-toxic and tasteless, nothing
Environmental pollution has persistent infection power to pest, and pest can continuously disseminate propagation once infection, and no agriculture is residual, even if applying white deadlock
Any pesticide residue will not be caused by harvesting product after bacterium immediately, non-resistant, muscardine by under field conditions (factors) with pest
Body wall be infected by contact it is lethal, pest will not generate any resistance to it, use year after year, and effect is higher and higher instead, and ball spore is white
Stiff bacterium contains living body fungi and spore, after applying field, by suitable temperature, humidity, can continue flourish, enhancing is killed
Worm effect has regrowth, kills beneficial insect, pest total number different from what chemical pesticide was not distinguished between the enemy and ourselves, muscardine specificity
By force, non-target organism such as ladybug, Chrysopa and food aphid horsefly etc. beneficial insects are influenced smaller, with highly selective, therefore muscardine is
A kind of environment friendly agricultural, ideal pesticide, not only can safely and effectively control orchard insect pest, but also can protect the places such as orchard, avoid
Ecological environment is destroyed by pollution；Tuber of stemona grass, Ranunculaceae, delphinium are distributed widely in Central China Huang away from ground leave plant
River, Yangtze river basin each province, it is mostly wild in height above sea level 500~2800 meters of mountainous regions grass slope or hills sandy ground, main product Shaanxi, Shandong, peace
Emblem, Jiangsu, Zhejiang etc. save；Be used as medicine with root tuber, root tuber contain a variety of alkaloids, take orally can moistening lung to arrest cough, external application can delousing desinsection, tool
Itching-relieving action；When antibacterial action in vitro test, tuber of stemona decoction and the immersion liquid of radix stemonae tuberosae alcohol to various pathogens such as pneumococcus,
Beta hemolysis type streptococcus, meningococcus, staphylococcus aureus, staphylococcus albus and shigella dysenteriae, typhoid bacillus, pair
Typhoid bacillus, Escherichia coli, proteus, corynebacterium diphtheriae, pneumobacillus, yersinia pestis, bacillus anthracis, hay bacillus, and
Comma bacillus, Bacillus tuberculosis etc. have different degrees of bacteriostasis, and Radix stemonae japonicae infusion causes a disease to certain in vitro
Fungi has certain inhibiting effect；It is practiced and is tested, the present invention reaches 95% or more to the eradicative rate in orchard, is a kind of peace
Entirely, orchard is effectively killed, without drug resistance, the biological insecticides for avoiding environment from suffering contaminated prevention and treatment orchard insect pest.
It the composite can be widely applied to pest and disease control of fruit trees field.
Claims (7)
1. a kind of process for preparing bio-insecticide for preventing and treating orchard insect pest, it is characterised in that: the following steps are included:
(1) tuber of stemona grass block root slice is taken to decoct, root tuber and water quality ratio press 1:25~1:40, and water is cooked by slow fire 60 after opening~
90min is obtained by filtration tuber of stemona grassland liquid, is cooled to room temperature；
(2) tuber of stemona grassland liquid and water 1:25~1:40 in mass ratio are diluted, the tuber of stemona grassland liquid after being diluted is spare；
(3) beauveria bassiana is inoculated with, cultivated, obtain beauveria bassiana culture solution, beauveria bassiana spore in culture solution
Concentration be 100~120/ml；
(4) grinder slurry paste ground into juice will be used after the cutting of tuber of stemona grass blade leaf, mixes well, is being sent out with beauveria bassiana culture solution
Be sealed anaerobic fermentation in fermentation tank jointly, the mass ratio of cauline leaf starchiness juice and beauveria bassiana culture solution be 10:1~
15:1, the temperature of fermentation are 26 ± 2 DEG C, and the time of fermentation is 6~8 days；
(5) product after fermentation is filtered, obtains fermentation liquid, fermentation filter residue is obtained after filtering, it is spare；
(6) after according to volume ratio 1:25~1:40 of fermenation raw liquid and water dilution, the fermenation raw liquid after being diluted is spare.
2. the process for preparing bio-insecticide of prevention and treatment orchard insect pest according to claim 1, it is characterised in that: described hundred
Portion's grass is using radix stemonae tuberosae or Radix stemonae japonicae or radix stemonae sessilifoliae.
3. a kind of biological insecticides for preventing and treating orchard insect pest, it is characterised in that: by prevention and treatment orchard of any of claims 1 or 2 worm
Harmful process for preparing bio-insecticide is made.
4. a kind of application method of the biological insecticides of prevention and treatment orchard insect pest as claimed in claim 3, it is characterised in that: will dilute
1:1 is mixed tuber of stemona grass block stoste afterwards by volume with the fermenation raw liquid after dilution；After mixing to fruit tree carry out sprinkling or
It brushes.
5. a kind of application method of the biological insecticides of prevention and treatment orchard insect pest as claimed in claim 3, it is characterised in that: will dilute
1:1 is mixed tuber of stemona grass block stoste afterwards by volume with the fermenation raw liquid after dilution；To the original of trunks of fruit trees after mixing
Hole is irrigated or is irrigated by Manual punching.
6. a kind of application method of the biological insecticides of prevention and treatment orchard insect pest as claimed in claim 3, it is characterised in that: will dilute
1:1 is mixed tuber of stemona grass block stoste afterwards by volume with the fermenation raw liquid after dilution；After mixing again with fermentation filter residue, soil
Secondary mixing, and the hole on trunks of fruit trees is blocked.
7. a kind of application method of the biological insecticides of prevention and treatment orchard insect pest as claimed in claim 3, it is characterised in that: will dilute
1:1 is mixed tuber of stemona grass block stoste afterwards by volume with the fermenation raw liquid after dilution；After mixing again with fermentation filter residue, soil
Secondary mixing, and trunks of fruit trees is brushed.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112106789A (en) * | 2020-09-11 | 2020-12-22 | 安徽农业大学 | Application of delphinium ethyl acetate extract in preventing and treating diamond back moth |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108850034A (en) * | 2018-05-03 | 2018-11-23 | 蒋德云 | A kind of drug and preparation method of deinsectization |
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2018
- 2018-12-11 CN CN201811507008.1A patent/CN109362814A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108850034A (en) * | 2018-05-03 | 2018-11-23 | 蒋德云 | A kind of drug and preparation method of deinsectization |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112106789A (en) * | 2020-09-11 | 2020-12-22 | 安徽农业大学 | Application of delphinium ethyl acetate extract in preventing and treating diamond back moth |
| CN112106789B (en) * | 2020-09-11 | 2021-08-24 | 安徽农业大学 | Application of delphinium ethyl acetate extract in preventing and treating diamond back moth |
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Application publication date: 20190222 |