CN103651576B - A kind of honeyization nicotine insecticidal solution and preparation method thereof - Google Patents
A kind of honeyization nicotine insecticidal solution and preparation method thereof Download PDFInfo
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- CN103651576B CN103651576B CN201310551624.8A CN201310551624A CN103651576B CN 103651576 B CN103651576 B CN 103651576B CN 201310551624 A CN201310551624 A CN 201310551624A CN 103651576 B CN103651576 B CN 103651576B
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Abstract
The present invention relates to biological pesticide technical field, disclose a kind of honeyization nicotine insecticidal solution and preparation method thereof.The present invention soaks to obtain the nicotine aqueous solution by getting waste/hypo-tobacco leaf, and in the described nicotine aqueous solution, add EM bacterium stoste or EM bacterium stoste and brown sugar, anaerobic fermentation, obtains sweetization nicotine.Honeyization nicotine insecticidal solution of the present invention can be used for flower garden ornamental plants cultivation and organic agronomic crop is produced, and is one green bio agricultural chemicals completely.
Description
Technical field
The present invention relates to biological pesticide technical field, more specifically, relate to a kind of honeyization nicotine insecticidal solution and preparation method thereof.
Background technology
Nicotine is a kind of natural green bio agricultural chemicals, has the feature of efficient, low toxicity, noresidue.Determination of Nicotine Content of Tobacco is generally 3% ~ 4%, because nicotine is very easily oxidized, usual inorganic acid (sulfuric acid, hydrochloric acid, nitric acid etc.) absorb nicotine make with salt volume form exist nicotine insecticide, mass percent concentration be 40% nicotine sulphate can be used for prepare organic farming use efficient insecticide liquid.Prove according to field experiment, the nicotine sulphate aqueous solution of 0.03% ~ 0.1% mass percent concentration is utilized to spray farmland economic crops, every mu of ground consumption 0.05 ~ 0.15 kilo sulfuric acid nicotine, reaches more than 90% to the killing effect of the multiple kinds of crops insects such as aphid, rice fulgorid, cabbage caterpillar, corn borer, European red mite, tea moth.
Patent CN102986744A discloses a kind of agricultural insecticide and preparation method, and specifically disclosing with tobacco waste is raw material, makes by inorganic acid the nicotine insecticide existed in a salt form that concentration is 70-80%.
Patent CN1098251A discloses a kind of insecticide of containing nicotine sulfate, specifically discloses each component ratio of nicotine sulphate and benzyl oxide pyrethrin pesticide mixture.
Prior art needs to use the chemical reagent such as inorganic acid (as sulfuric acid), and production cost is higher, and equipment investment is comparatively large, and operating process is complicated, and need administer the sewage produced in production process.
Summary of the invention
The object of this invention is to provide a kind of honeyization nicotine insecticidal solution, described honeyization nicotine insecticidal solution has the insecticidal effect higher than common nicotine solution, and the better therefore storage life of the stability of nicotine is longer.
Another object of the present invention is to provide a kind of preparation method of honeyization nicotine insecticidal solution.
Above-mentioned purpose of the present invention is achieved by following technical solution:
A kind of honeyization nicotine insecticidal solution, described honeyization nicotine insecticidal solution prepares by the following method: get waste/hypo-tobacco leaf and soak to obtain the nicotine aqueous solution, in the described nicotine aqueous solution, add EM bacterium stoste, anaerobic fermentation, obtains described honeyization nicotine insecticidal solution.
Inventor finds; effective microbe in EM bacterium stoste can carry out anaerobic fermentation to the soluble protein of the nicotine aqueous solution and carbohydrate; in anaerobic fermentation process, antioxidant protein and low molecular polyphenoils can be generated, can available protecting nicotine stability and improve insecticidal effect.
A kind of honeyization nicotine insecticidal solution, described honeyization nicotine insecticidal solution prepares by the following method: get waste/hypo-tobacco leaf and soak to obtain the nicotine aqueous solution, in the described nicotine aqueous solution, add EM bacterium stoste and brown sugar, anaerobic fermentation, obtains described honeyization nicotine insecticidal solution.
Inventor also finds, carries out adding brown sugar in the process of anaerobic fermentation, can strengthen the anaerobic fermentation effect of the nicotine aqueous solution further in use EM bacterium stoste.Inventor infers, the brown sugar added can for beneficial microbe as saccharomycete, bacillus, lactic acid bacteria etc. provide energy needed for growth, suppress harmful microbe growth, thus prevent the nicotine aqueous solution putrid and deteriorated, strengthen the anaerobic fermentation effect of the nicotine aqueous solution.
Described honeyization nicotine insecticidal solution prepares as follows:
S1. get waste/hypo-tobacco leaf, soak, obtain the nicotine aqueous solution, the mass ratio of described water and waste/hypo-tobacco leaf is 5 ~ 10:1;
S2. add EM bacterium stoste to the described nicotine aqueous solution, the mass ratio of the described nicotine aqueous solution and described EM bacterium stoste is 20 ~ 30:1;
S3. get step S2. gained solution, under being placed in 25 ~ 30 DEG C of conditions, anaerobic fermentation 7 ~ 15 days, obtains sweetization nicotine insecticidal solution.
Described honeyization nicotine insecticidal solution can also prepare as follows:
S1. get waste/hypo-tobacco leaf, soak, obtain the nicotine aqueous solution, the mass ratio of described water and waste/hypo-tobacco leaf is 5 ~ 10:1;
S2. add EM bacterium stoste and brown sugar to the described nicotine aqueous solution, the mass ratio of the described nicotine aqueous solution and described EM bacterium stoste is 20 ~ 30:1;
S3. get step S2. gained solution, under being placed in 25 ~ 30 DEG C of conditions, anaerobic fermentation 7 ~ 15 days, obtains sweetization nicotine insecticidal solution.
Alternatively, in step S1., described water can be clean well water or river.
As a kind of preferred version, in step S1., the time of described immersion is preferably 24 ~ 48 hours.
As a kind of preferred version, when step S2. is added with brown sugar, the mass ratio of the nicotine aqueous solution, EM bacterium stoste, brown sugar is preferably 20 ~ 30:1:1.
Described honeyization nicotine insecticidal solution is brown color, and the sweet taste of the sweet acid that described honeyization nicotine insecticidal solution has one strong, the pH of described honeyization nicotinic liquid is 3 ~ 5.
Honey nicotinic liquid well water the present invention prepared or river dilution 3 ~ 5 times are directly sprayed on economic crops afterwards, effective microbe in honeyization nicotine insecticidal solution can stimulate blade face and the root system of crops, improve the vigor of crops, its diseases and insect pests resistance of further enhancing, improve the quality and yield of crops, can more than 90% be reached to the killing effect of crop pests simultaneously.
The invention has the beneficial effects as follows:
1, utilize EM bacterium to carry out anaerobic fermentation to the nicotine aqueous solution, both effectively protect the stability of nicotine, in turn enhance the insecticidal effect of nicotine;
2, the effective microbe in the honey nicotine insecticidal solution after the fermentation of EM bacterium can stimulate blade face and the root system of crops, improves the vigor of crops, improves the quality and yield of crops further;
3, production cost is lower, simple to operate, environmentally friendly;
4, can be used for flower garden ornamental plants cultivation and organic agronomic crop production, is one green bio agricultural chemicals completely.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further details.Embodiment, only for explaining the present invention, does not limit in any form content of the present invention.
Embodiment 1
S1. utilize clean well water to soak waste/hypo-tobacco leaf 48 hours, the mass ratio of well water and waste/hypo-tobacco leaf is 5:1, obtains the nicotine aqueous solution;
S2. in the nicotine aqueous solution, EM bacterium stoste and brown sugar is added, the nicotine aqueous solution: EM bacterium stoste: brown sugar=20:1:1(mass ratio);
S3., under room temperature 25 DEG C of conditions, anaerobic fermentation 15 days, namely obtain honeyization nicotine insecticidal solution, its color is brown color, and have the sweet tart flavour that one is dense, pH value is about 3.3.
Embodiment 2
S1. utilize free of contamination river to soak waste/hypo-tobacco leaf 36 hours, the mass ratio of river and waste/hypo-tobacco leaf is 8:1, obtains the nicotine aqueous solution;
S2. in the nicotine aqueous solution, EM bacterium stoste and brown sugar is added, the nicotine aqueous solution: EM bacterium stoste: brown sugar=25:1:1(mass ratio);
S3., under room temperature 28 DEG C of conditions, anaerobic fermentation 13 days, namely obtain honeyization nicotine insecticidal solution, its color is brown color, and have the sweet tart flavour that one is dense, pH value is about 3.5.
Embodiment 3
S1. utilize clean well water to soak waste/hypo-tobacco leaf 24 hours, the mass ratio of well water and waste/hypo-tobacco leaf is 10:1, obtains the nicotine aqueous solution;
S2. in the nicotine aqueous solution, EM bacterium stoste and brown sugar is added, the nicotine aqueous solution: EM bacterium stoste: brown sugar=30:1:1(mass ratio);
S3., under room temperature 30 DEG C of conditions, anaerobic fermentation 7 days, namely obtain honeyization nicotine insecticidal solution, its color is brown color, and have the sweet tart flavour that one is dense, pH value is about 3.8.
Embodiment 4
S1. utilize free of contamination river to soak waste/hypo-tobacco leaf 36 hours, the mass ratio of river and waste/hypo-tobacco leaf is 8:1, obtains the nicotine aqueous solution;
S2. in the nicotine aqueous solution, EM bacterium stoste is added, the nicotine aqueous solution: EM bacterium stoste=25:1(mass ratio);
S3. under room temperature 28 DEG C of conditions, anaerobic fermentation 15 days, namely obtain honeyization nicotine insecticidal solution, its color is brown color, slightly sweet tart flavour, and pH value is about 5.0;
Comparative example 1
S1. utilize free of contamination river to soak waste/hypo-tobacco leaf 36 hours, the mass ratio of river and waste/hypo-tobacco leaf is 8:1, obtains the nicotine aqueous solution;
S2. in the nicotine aqueous solution, brown sugar is added, the nicotine aqueous solution: brown sugar=30:1(mass ratio);
S3., under room temperature 28 DEG C of conditions, place 13 days, its solution is brown color, has the sweet taste of one brown sugar, but without obvious tart flavour, pH value is about 6.5;
Embodiment 5 nicotine insecticidal solution effect test
Example 1 ~ 4 and comparative example 1 prepare the nicotine insecticidal solution 1 kilogram of gained respectively, dilute 5 times be directly sprayed on afterwards on tobacco with well water, as shown in table 1 to the killing effect of crop pests.
The insecticidal effect of the different nicotine insecticidal solution of table 1
Embodiment 6 nicotine stability is tested
Example 1-4 and comparative example 1 prepare the nicotine insecticidal solution 100ml of gained respectively, sealing is preserved, number consecutively is 1,2,3,4,5, utilize continuous flow method to measure nicotine content (YC/T160-2002), test each nicotine insecticidal solution respectively and place 1 day, 3 days, 10 days, after 30 days, in each nicotine insecticidal solution, the content of nicotine, the results are shown in Table 2.Can find out, without the process of EM bacterium stoste anaerobic fermentation the nicotine aqueous solution in placement after 30 days, significantly declining appears in nicotine content.And described in the application, although declining also appears in nicotine content in honeyization nicotine, amplitude of variation is little.
The different nicotine insecticidal solution of table 2 places the nicotine content (%) after different time
Claims (6)
1. a honeyization nicotine insecticidal solution, is characterized in that, described honeyization nicotine insecticidal solution prepares as follows:
S1. get waste/hypo-tobacco leaf, soak, obtain the nicotine aqueous solution, the mass ratio of described water and waste/hypo-tobacco leaf is 5 ~ 10:1;
S2. add EM bacterium stoste to the described nicotine aqueous solution, the mass ratio of the described nicotine aqueous solution and described EM bacterium stoste is 20 ~ 30:1;
S3. get step S2. gained solution, under being placed in 25 ~ 30 DEG C of conditions, anaerobic fermentation 7 ~ 15 days, obtains sweetization nicotine insecticidal solution;
In step S1., the time of described immersion is 24 ~ 48 hours.
2. a honeyization nicotine insecticidal solution, is characterized in that, described honeyization nicotine insecticidal solution prepares as follows:
S1. get waste/hypo-tobacco leaf, soak, obtain the nicotine aqueous solution, the mass ratio of described water and waste/hypo-tobacco leaf is 5 ~ 10:1;
S2. add EM bacterium stoste and brown sugar to the described nicotine aqueous solution, the mass ratio of the described nicotine aqueous solution and described EM bacterium stoste is 20 ~ 30:1;
S3. get step S2. gained solution, under being placed in 25 ~ 30 DEG C of conditions, anaerobic fermentation 7 ~ 15 days, obtains sweetization nicotine insecticidal solution;
In step S1., the time of described immersion is 24 ~ 48 hours.
3. honeyization nicotine insecticidal solution according to claim 1 or 2, is characterized in that, in step S1., described water is clean well water or river.
4. honeyization nicotine insecticidal solution according to claim 2, it is characterized in that, in step S2., the mass ratio of the nicotine aqueous solution, EM bacterium stoste, brown sugar is 20 ~ 30:1:1.
5. honeyization nicotine insecticidal solution according to claim 1 or 2, is characterized in that, the color of described honeyization nicotine insecticidal solution is brown color.
6. honeyization nicotine insecticidal solution according to claim 1 or 2, is characterized in that, the pH value of described honeyization nicotine insecticidal solution is 3 ~ 5.
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| US10201163B1 (en) | 2015-01-13 | 2019-02-12 | Charles Russell Berg | Honey based biocide composition |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102265908A (en) * | 2010-06-04 | 2011-12-07 | 郑州大学 | Tobacco matrix composite biopesticide and preparation method thereof |
| CN102442844A (en) * | 2010-10-09 | 2012-05-09 | 云南昆船设计研究院 | Comprehensive resource treatment process of tobacco wastes |
| CN102851318A (en) * | 2011-06-29 | 2013-01-02 | 郑州大学 | Resource utilization method for combined production of biogas and cultivation matrix through utilizing tobacco stalk fermentation |
| CN102887754A (en) * | 2012-11-05 | 2013-01-23 | 师宗海利食品有限责任公司 | Process for producing marsh gas and bioorganic fertilizer by utilizing tobacco waste |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52120199A (en) * | 1976-03-31 | 1977-10-08 | Japan Tobacco Inc | Improvement of tobacco taste |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102265908A (en) * | 2010-06-04 | 2011-12-07 | 郑州大学 | Tobacco matrix composite biopesticide and preparation method thereof |
| CN102442844A (en) * | 2010-10-09 | 2012-05-09 | 云南昆船设计研究院 | Comprehensive resource treatment process of tobacco wastes |
| CN102851318A (en) * | 2011-06-29 | 2013-01-02 | 郑州大学 | Resource utilization method for combined production of biogas and cultivation matrix through utilizing tobacco stalk fermentation |
| CN102887754A (en) * | 2012-11-05 | 2013-01-23 | 师宗海利食品有限责任公司 | Process for producing marsh gas and bioorganic fertilizer by utilizing tobacco waste |
Non-Patent Citations (2)
| Title |
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| 沼液复合型杀虫剂研究;张全国 等;《农业工程学报》;20060630;第22卷(第6期);第157-160页 * |
| 烟叶下脚料有机肥和有机无机复混肥应用效果研究;张翔 等;《河南农业科学》;20080831(第8期);第84-86页 * |
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