CN105028108A - Planting method for controlling infection of citrus tristeza virus on newly-grown citrus seedlings - Google Patents

Planting method for controlling infection of citrus tristeza virus on newly-grown citrus seedlings Download PDF

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Publication number
CN105028108A
CN105028108A CN201510536645.1A CN201510536645A CN105028108A CN 105028108 A CN105028108 A CN 105028108A CN 201510536645 A CN201510536645 A CN 201510536645A CN 105028108 A CN105028108 A CN 105028108A
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China
Prior art keywords
citrus
oranges
tangerines
seedling
tristeza virus
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CN201510536645.1A
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Inventor
毛润乾
郑基焕
张宇宏
董冰雪
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Guangdong Entomological Institute
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Guangdong Entomological Institute
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Priority to CN201510536645.1A priority Critical patent/CN105028108A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods

Abstract

The invention discloses a planting method for controlling the infection of the citrus tristeza virus on newly-grown citrus seedlings. The planting method includes the steps that terrestrial citrus seedlings are loaded with a nutrition pot filled with nutrition soil or field soil, then the nutrition pot loaded with the terrestrial citrus seedlings is put into a greenhouse to be cultivated in a centralized mode or centralized in farmland to be cultivated, a black mulching film or a light reflecting mulching film is used for covering the ground of the farmland, the farmland is relatively independent, and citrus planting does not exist in the far peripheral range; the young shoot emerging time, the shoot emerging amount and the young shoot growing height of each batch are recorded; the pest and disease damage occurrence on the citrus seedlings is regularly detected, spraying preventing and controlling are conducted purposefully, a diaphorina citri killing pesticide is sprayed in the shoot stage, meanwhile, pests such as aphids and leaf miners are controlled, and disease preventing and controlling are conducted according to a conventional method; the tristeza virus is detected every year, and if trees infected with the tristeza virus are found, the tristeza virus infected with the tristeza virus are removed immediately; the citrus seedlings are fixedly planted into a field after two years. The planting method can effectively lower the risk of infecting the citrus tristeza virus and even eliminate the citrus tristeza virus.

Description

A kind of implantation methods controlling oranges and tangerines newborn seedling infection Citrus Huanglongbing pathogen
Technical field:
The invention belongs to control of agricultural pest field, be specifically related to a kind of implantation methods controlling oranges and tangerines newborn seedling infection Citrus Huanglongbing pathogen.
Background technology:
China is Orange Producing big country in the world, and Citrus Industry is China's Major agro-industry, occupies extremely important status in agricultural economy, in promotion increasing peasant income, increase social employment and building Socialist New, play extremely important effect.Oranges and tangerines damage by disease and insect is the major obstacles of Aspects In The Development of Citrus Industry, is wherein the most seriously called as the Citrus Huanglongbing pathogen of oranges and tangerines " cancer ".Along with the fast development of Orange Producing, the generation of Citrus Huanglongbing pathogen and cause harm more and more serious.This disease is that one causes harm great contagious disease, germ is propagated by diaphorina citri, its popular duration is long, morbidity scope is wide, the incidence of disease is high, cause China's most area oranges and tangerines life-span short, yield poorly, production cost is high, annual economic loss, up to multi-million dollar, seriously perplexs and restricts the development of Citrus Industry in China and even world wide.
Guangdong Province Insects Research Institute compares systematic research in yellow twig, and result shows that yellow twig can effectively control.At present, there is serious region in Citrus Huanglongbing pathogen, and some peasant household all cuts down, and prepares again to plant, and also current oranges and tangerines price good opportunity is also aimed in some other agricultural lands, prepares to greatly develop oranges and tangerines plantation.But we investigate discovery, although we advocate use container seedlings, due to Lu Sheng seedling cheap (container seedlings 10-15 unit/, Lu Sheng seedling 5 yuan /), the common way in novel species orchard buys Lu Shengmiao, is then directly transplanted in orchard.There is following problem in this method: when (1) Lu Sheng shoot root ties up to transplanting, be subject to destruction in various degree, therefore survival rate can be relatively low, and important time root regeneration needs the time, therefore grows slower.(2) slow owing to growing, just bear fruit gradually after the 3rd year, the therefore normal negligence of management of peasant household, in fruit setting garden, diaphorina citri causes harm seriously, and before not bearing fruit, yellow twig just infects.Find at riverhead one novel species orchard survey, novel species 1 year orchard, yellow twig incidence reaches 20%.(3) novel species orchard, due to Miao Tai little, weeding pressure is larger.(4) pest management difficulty is large, and because seedling is little, plantation dispersion, therefore pest management difficulty is large, and cost is high, weak effect, and this is also that diaphorina citri control is not in place, the incident reason of Citrus Huanglongbing pathogen.
As can be seen here, resolving the management work of oranges and tangerines before Seedling Stage result, is that can oranges and tangerines plant one of important technology that bring benefits.
Summary of the invention:
The object of this invention is to provide and a kind ofly effectively can reduce the risk that Citrus Huanglongbing pathogen is even eliminated in infection, and simple to operate, effectively and the newborn seedling of the control oranges and tangerines of environmental protection infect the implantation methods of Citrus Huanglongbing pathogen.
The newborn seedling of control oranges and tangerines of the present invention infects the implantation methods of Citrus Huanglongbing pathogen, it is characterized in that, comprises the following steps:
The plantation of a, oranges and tangerines Lu Sheng seedling: the oranges and tangerines Lu Sheng seedling nutritive cube being filled with Nutrition Soil or field soil is loaded, then the nutritive cube of the oranges and tangerines Lu Sheng seedling be mounted with is concentrated in booth cultivation or concentrate in farmland and cultivate, the ground in farmland covers black mulch film or Reflecting mulch film, described farmland is relatively independent, around plants without oranges and tangerines in scope far away;
B, oranges and tangerines seedling young sprout manage: record is often criticized young sprout and gone out the time, goes out tip amount and young sprout growing height, to assess growing state and to formulate extermination of disease and insect pest strategy;
C, oranges and tangerines pest management: on periodic detection oranges and tangerines seedling, a situation arises for damage by disease and insect, the control of specific aim spray medicine, tip phase spray wine diaphorina citri kills medicament, and control the insect such as aphid, leaf miner, disease control is carried out according to a conventional method simultaneously;
D, detect yellow twig every year, as found yellow twig tree, then root out immediately and infected yellow twig tree;
E, after 2 years by the field planting of oranges and tangerines seedling to large Tanaka.
Described nutritive cube is preferably circular or square heightening type nutritive cube.
Described booth preferably has the booth of fly net equipment.
Young sprout management can be oranges and tangerines transplantation of seedlings field planting provides basic data to large Tanaka.
Described diaphorina citri is killed medicament and is comprised Imidacloprid Insecticide, mineral oil (or vegetable oil or waste oil) insecticide, doublely to eliminate aphis and leaf miner medicament is Avermectin etc.On periodic detection oranges and tangerines seedling, a situation arises is 1 time weekly for damage by disease and insect, can stochastical sampling investigation, its mainly for be canker, anthraenose, can spraying fungicide in good time, bactericide comprises copper agent etc.
Described annual detection yellow twig is preferably annual at least sampling Detection 2 yellow twigs, can detect by PCR method and starch coloration method (patent ZL201310244391.7).
Before the present invention effectively solves oranges and tangerines Seedling Stage result oranges and tangerines management work in there is technical problem, effectively can reduce the risk that Citrus Huanglongbing pathogen is even eliminated in infection.Method of the present invention has advantage that is simple to operate, effective, environmental protection, can reach the target of the effective prevention and control of the early stage yellow twig of plantation.
Specific implementation method:
Following examples further illustrate of the present invention, instead of limitation of the present invention.
Embodiment 1:
Farm, faithful and honest town, riverhead, zoology breeding in farm, has live pig 2000, chicken 3000, goose 1500, peanut cultivation 60 mu, 120 mu, paddy rice, vegetables 100 mu, olecranon peach 2000 strain.Plant sugar orange 10000 strain simultaneously, plant 1 year, and 10000 strains can plant land used.Farm is relatively independent, near do not have oranges and tangerines to plant.
Planted sugar orange seedling because Miao little Wei result, manage poor, weeds are many, and tree vigo(u)r is weak, and insect occurs serious, has aphid, leaf miner, two spotted spider mite, Papilio xuthus etc., find diaphorina citri adult simultaneously.Weeding expense is every mu each 35 yuan, 1 year secondary, totally 70 yuan.Spray medicine is less, and fertilising adopts methane liquid in farm.
1, detect sample investigation with PCR and planted sugar orange yellow twig infection conditions, concluding shows that 20% sugar orange infects yellow twig.
2, use starch coloration rapid assay methods, to planting sugar orange examination one by one, rooting out and infecting yellow twig tree.Diseased plant is burnt, and tree well spills quicklime.Strengthen management simultaneously, kill diaphorina citri.
3, sugar orange seedling is bought.Due to Cost Problems, farmer still buys Lu Shengmiao, and the 1st spring phase bought 5000 strains.
4, circular heightening type nutritive cube is selected to load plantation sugar orange Lu Sheng seedling, load with common field soil with above fertilizer at the bottom of the alms bowl of heightening type nutritive cube, then the 4900 strain sugar orange Lu Sheng seedlings that the first phase is bought are cultivated with the heightening type nutritive cube of this circle, concentrate and be planted in existing green house of vegetables, booth has fly net.Select 100 strains for Germicidal efficacy in addition, wherein the 50 strain sugar orange Lu Sheng seedlings heightening type nutritive cube of the circle that Nutrition Soil is housed is cultivated, cultivate with the heightening type nutritive cube of the circle of common field soil with above fertilizer at the bottom of other 50 strain sugar orange Lu Sheng seedling alms bowls, be also planted in above-mentioned existing green house of vegetables.
The heightening type nutritive cube of the 5000 strain sugar orange Lu Sheng seedlings circles that the second phase is bought is cultivated, load with common field soil with above fertilizer at the bottom of its alms bowl, then concentrate and be planted in former peanut ground, covered ground black mulch film, this peanut ground is relatively independent, around plants without oranges and tangerines in 300m-1000m.
5, young sprout management: 100 strains of record Germicidal efficacy are often criticized young sprout and gone out the time, go out tip amount and young sprout growing height, to assess growing state and to formulate extermination of disease and insect pest strategy.
6, oranges and tangerines pest management: on periodic detection oranges and tangerines seedling, a situation arises for damage by disease and insect, the control of specific aim spray medicine.Tip phase spray wine diaphorina citri kills medicament, controls the insect such as aphid, leaf miner simultaneously.Diaphorina citri medicament comprises Imidacloprid Insecticide, mineral oil (or vegetable oil or waste oil) insecticide, doublely to eliminate aphis and leaf miner medicament is Avermectin etc.Periodic detection is 1 time weekly, can stochastical sampling investigation.Plant disease management, can spraying fungicide in good time mainly for canker, anthraenose, and bactericide comprises copper agent etc.Disease control is carried out according to a conventional method.
7, every year concentrate the sugar orange Lu Sheng seedling sampling Detection yellow twig 2 times of cultivation to booth and peanut, detection method can use PCR method, also can use starch coloration method, method detection disclosed in patent ZL201310244391.7.
8, after 2 years, spring concentrates the sugar orange seedling field planting of cultivation to large Tanaka by above-mentioned at booth or peanut.
9, implementation result performance:
(1) weed killer herbicide use cost and artificial weeding cost are saved completely.5000 strains as plantation to large Tanaka account for 100 mu, weeding cost is 100 mu * 35 yuan/times * 2 times * 2 years=14000 yuan.
(2) young sprout goes out the more original increase by 25% of tip amount, and young sprout growing height increases by 18%.Young sprout goes out the tip neatly simultaneously, is beneficial to management.
(3) after field planting, the direct planting survival rates of survival rate 99.99%, Lu Shengmiao is about 90-95%.Invention increases 5-10%.
(4) Pest management pressure is low.In booth, only have citrus red mite to cause harm, then cause harm extremely light, spray wine mineral oil (or vegetable oil or waste oil) insecticide or can prevent and treat with methane liquid mixing sprinkling.Concentrate growing area peanut, can diaphorina citri be prevented and treated 1-2 time at young sprout germination period spray Imidacloprid, the young sprout phase leaf miner, aphid also has and causes harm, interpolation Avermectin and mineral oil can be prevented and treated.Pest management cost comparatively land for growing field crops province 78% in booth, the larger Tanaka's cost savings about 46% of vacant lot plantation.
(5) plant disease management.In booth, the young sprout phase adds copper agent, as 30%DTWP500 doubly uses, and the effectively generation of prevention citrus bacterial canker disease.Or spray the bactericidal agent for preventing and treating anthraenose such as carbendazim, benzene first Fluoxastrobin to cause harm.Vacant lot manages same booth, and each tip phase spray medicine 2 times, outer rim mandarin tree needs emphasis to spray medicine.
(6) Citrus Huanglongbing pathogen testing result.Within 2 years, detect 4 times altogether, each sampling 200 strains, random sampling, result shows that yellow twig incidence is 0.
(7) can to bear fruit then after field planting 10-50 jin.
(8) brief summary: young sprout of the present invention goes out the more original increase by 25% of tip amount, young sprout growing height increases by 18%, and plant strain growth is healthy; Survival rate 99.99%; Effective guarantee economic well-being of workers and staff, can bear fruit after field planting then, obtains income; Management cost significantly reduces management cost, and wherein 100% reduces weeding cost, and 46-78% reduces pest management cost; Prevention Citrus Huanglongbing pathogen method safety is effective, and yellow twig incidence is 0; Preventive effect can reach 100%.

Claims (5)

1. control the implantation methods that the newborn seedling of oranges and tangerines infects Citrus Huanglongbing pathogen, it is characterized in that, comprise the following steps:
The plantation of a, oranges and tangerines Lu Sheng seedling: the oranges and tangerines Lu Sheng seedling nutritive cube being filled with Nutrition Soil or field soil is loaded, then the nutritive cube of the oranges and tangerines Lu Sheng seedling be mounted with is concentrated in booth cultivation or concentrate in farmland and cultivate, the ground in farmland covers black mulch film or Reflecting mulch film, described farmland is relatively independent, around plants without oranges and tangerines;
B, oranges and tangerines seedling young sprout manage: record is often criticized young sprout and gone out the time, goes out tip amount and young sprout growing height;
C, oranges and tangerines pest management: on periodic detection oranges and tangerines seedling, a situation arises for damage by disease and insect, the control of specific aim spray medicine, tip phase spray wine diaphorina citri kills medicament, and control the insect such as aphid, leaf miner, disease control is carried out according to a conventional method simultaneously;
D, detect yellow twig every year, as found yellow twig tree, then root out immediately and infected yellow twig tree;
E, after 2 years by the field planting of oranges and tangerines seedling to large Tanaka.
2. implantation methods according to claim 1, is characterized in that, described nutritive cube is circular or square heightening type nutritive cube.
3. implantation methods according to claim 1, is characterized in that, described booth is the booth with fly net equipment.
4. implantation methods according to claim 1, is characterized in that, it is Imidacloprid Insecticide or mineral oil insecticide that described diaphorina citri kills medicament; Described control aphid and the medicament of leaf miner are Avermectin.
5. implantation methods according to claim 1, is characterized in that, described annual detection yellow twig is annual at least sampling Detection 2 yellow twigs, adopts PCR method and starch coloration method to detect.
CN201510536645.1A 2015-08-26 2015-08-26 Planting method for controlling infection of citrus tristeza virus on newly-grown citrus seedlings Pending CN105028108A (en)

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Cited By (7)

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Publication number Priority date Publication date Assignee Title
CN105684795A (en) * 2016-01-25 2016-06-22 柳州市净元生物科技有限公司 Method for preventing and treating huanglongbing by means of novel microorganisms
CN107926399A (en) * 2017-12-07 2018-04-20 岑溪市糯垌新科柑水果专业合作社 The pest control method of luxuriant paddy mandarin orange
CN108064891A (en) * 2017-12-01 2018-05-25 钟山县德福农产品有限公司 The pesticide control of Citrus Huanglongbing pathogen
CN108887059A (en) * 2018-07-04 2018-11-27 广西农垦国有明阳农场 A kind of fertile mandarin orange net canopy cultivation technique
CN109964722A (en) * 2019-04-29 2019-07-05 赣州市果树植保站 A kind of safe seedlings of net canopy temporary planting reseed prevention and control Citrus Huanglongbing pathogen technology
CN111149603A (en) * 2020-02-03 2020-05-15 广西特色作物研究院 Prevention and treatment method for citrus sapling yellow shoot
CN113207514A (en) * 2021-05-11 2021-08-06 新兴县天绿农产品专业合作社 Method for preventing citrus yellow shoot infection of citrus trees

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105684795A (en) * 2016-01-25 2016-06-22 柳州市净元生物科技有限公司 Method for preventing and treating huanglongbing by means of novel microorganisms
CN105684795B (en) * 2016-01-25 2019-04-09 柳州市净元生物科技有限公司 A method of yellow twig is prevented and treated using Novel microbial organic fertilizer
CN108064891A (en) * 2017-12-01 2018-05-25 钟山县德福农产品有限公司 The pesticide control of Citrus Huanglongbing pathogen
CN107926399A (en) * 2017-12-07 2018-04-20 岑溪市糯垌新科柑水果专业合作社 The pest control method of luxuriant paddy mandarin orange
CN108887059A (en) * 2018-07-04 2018-11-27 广西农垦国有明阳农场 A kind of fertile mandarin orange net canopy cultivation technique
CN109964722A (en) * 2019-04-29 2019-07-05 赣州市果树植保站 A kind of safe seedlings of net canopy temporary planting reseed prevention and control Citrus Huanglongbing pathogen technology
CN111149603A (en) * 2020-02-03 2020-05-15 广西特色作物研究院 Prevention and treatment method for citrus sapling yellow shoot
CN113207514A (en) * 2021-05-11 2021-08-06 新兴县天绿农产品专业合作社 Method for preventing citrus yellow shoot infection of citrus trees
CN113207514B (en) * 2021-05-11 2022-10-11 新兴县天绿农产品专业合作社 Method for preventing citrus yellow shoot infection of citrus trees

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