CN107347942A - A kind of beauveria bassiana microbial pesticide and preparation method thereof - Google Patents
A kind of beauveria bassiana microbial pesticide and preparation method thereof Download PDFInfo
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- CN107347942A CN107347942A CN201710714107.6A CN201710714107A CN107347942A CN 107347942 A CN107347942 A CN 107347942A CN 201710714107 A CN201710714107 A CN 201710714107A CN 107347942 A CN107347942 A CN 107347942A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom not containing sulfur-to-oxygen bonds, e.g. polysulfides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a kind of beauveria bassiana microbial pesticide and preparation method thereof, the raw material of beauveria bassiana microbial pesticide includes：Beauveria bassiana, nicotine, diatomite, Metarhizium anisopliae, baicalein, bacteriophagic Bdellovibrio, talcum powder, bacillus, vermiculite, sodium carboxymethylcellulose, plant growth regulator, aluminum oxide, baicalein, garlic oil, matrine, starch, round onions, oxymycin, streptomysin and oneself, chrysanthemum height, Phytolacca acinosa, the rhizome of Chinese monkshood, the coptis, cordate houttuynia, camphor, folium artemisiae argyi, Chinese violet, trace element compound.The raw materials used proportioning of agricultural chemicals of the present invention is simple, and green, cost is cheap；The immunocompetence of plant can be improved, pollution will not be brought to ecological environment, and active ingredient complexity is various so that insect can not develop immunity to drugs to one or more of compositions therein, and so as to receive preferable prevention effect, manufacture craft is simply, conveniently.
Description
Technical field
The present invention relates to technical field of pesticide, specifically a kind of beauveria bassiana microbial pesticide and preparation method thereof.
Background technology
At present, agricultural maintenance using high poison, medium toxic pesticides and other agricultural chemicals for influenceing health and ecological environment security as
It is main, to carry out the daily insect pest preventing and controlling in park maintenance.Desinsection is mainly with organic phosphates, the cruel class of carbamic acid, pyrethroid wine
Based on the agricultural chemicals such as class, sterilization is more based on the agricultural chemicals such as organic sulfur class, organic phosphates, the stupid class of substitution, benzo miaow mile class.
Such medicine had both polluted environmental disruption ecology, with the agricultural chemicals continuous use of kind or close preventing and treating mechanism, and easily made
Pest and disease damage is developed immunity to drugs, and lasting potential threat is easily caused to health of human body, especially for real estate gardens ring
The cell owner of the long-term close contact in border, health hazard are more direct.
Microbial pesticide (microbialpesticide) includes farm antibiotics and alive microbial agrochemical.It is micro- to utilize
Biology or its metabolite come prevent and treat the disease to harm the crops, worm, grass, the plague of rats and promote plant growth.It include with bacterium control worm,
Fungus treatment, with bacterium weeding etc..Biological pesticide is that one kind utilizes biological living or its metabolite to insect, germ, weeds, line
A kind of pesticidal preparations that the harmful organisms such as worm, muroid are prevented and treated, biological pesticide include microbial pesticide, botanical pesticide,
The class of animal pesticide and genetically modified organism agricultural chemicals etc. four, biological pesticide have the advantage that compared with chemical pesticide：1) selectivity is strong,
It is safer to people and animals and various beneficial organisms；2) it is small to eco-environmental impact.These microbial pesticides include bacterium, fungi,
Virus or its metabolin, such as Dipel, muscardine, nucleopolyhedrosis virus, jinggangmeisu, c-type clostridium botulinum exotoxin
Deng.As people are undoubtedly one of developing direction of agricultural chemicals from now on to the higher and higher requirement of environmental protection, microbial pesticide.
In known technology, the disease and pest control agent containing microorganism, selected microorganism is single or less.Preventing and treating
The scope of pest and disease damage is narrower, quality less stable, and the effect for preventing and treating pest and disease damage is unsatisfactory.Meanwhile it is more there is also access times,
Total Meteorological is more, and production technology is numerous and diverse, operates the deficiencies of inconvenient part.
The content of the invention
It is an object of the invention to provide the low beauveria bassiana microbial pesticide of a kind of less toxic wide spectrum, the resistance to the action of a drug and its system
Preparation Method, to solve the problems mentioned in the above background technology.
To achieve the above object, the present invention provides following technical scheme：
A kind of beauveria bassiana microbial pesticide, is made up of the raw material of following parts by weight：Beauveria bassiana 600-800 parts, nicotine
1-3 parts, diatomite 50-70 parts, Metarhizium anisopliae 6-8 parts, baicalein 15-25 parts, bacteriophagic Bdellovibrio 6-8 parts, talcum powder 6-8
Part, bacillus 8-12 parts, vermiculite 5-10 parts, sodium carboxymethylcellulose 1-3 parts, plant growth regulator 3-5 parts, aluminum oxide 5-
10 parts, baicalein 4-6 parts, garlic oil 0.5-2 parts, matrine 2-6 parts, starch 1-5 parts, round onions 20-30 parts, oxymycin 2-4 parts,
Streptomysin 1-3 parts and own 10-20 parts, chrysanthemum height 15-20 parts, Phytolacca acinosa 8-12 parts, rhizome of Chinese monkshood 10-20 parts, coptis 20-25 parts, fish raw meat
Careless 10-20 parts, camphor 3-5 parts, folium artemisiae argyi 3-5 parts, Chinese violet 1-3 parts, trace element compound 5-10 parts.
As the further scheme of the present invention：The beauveria bassiana microbial pesticide, by the raw material group of following parts by weight
Into：Beauveria bassiana 650-750 parts, nicotine 1.5-2.5 parts, diatomite 55-65 parts, Metarhizium anisopliae 6.5-7.5 parts, radix scutellariae
Plain 18-22 parts, bacteriophagic Bdellovibrio 6.5-7.5 parts, talcum powder 6.5-7.5 parts, bacillus 9-11 parts, vermiculite 6-9 parts, carboxymethyl
Sodium cellulosate 1.5-2.5 parts, plant growth regulator 3.5-4.5 parts, aluminum oxide 6-9 parts, baicalein 4.5-5.5 parts, garlic oil
1-1.5 parts, matrine 3-5 parts, starch 2-4 parts, round onions 22-28 parts, oxymycin 2.5-3.5 parts, streptomysin 1.5-2.5 parts and
Own 12-18 parts, chrysanthemum height 16-19 parts, Phytolacca acinosa 9-11 parts, rhizome of Chinese monkshood 12-18 parts, coptis 21-24 parts, cordate houttuynia 12-18 parts, camphor
3.5-4.5 parts, folium artemisiae argyi 3.5-4.5 parts, Chinese violet 1.5-2.5 parts, trace element compound 6-9 parts.
As the further scheme of the present invention：The beauveria bassiana microbial pesticide, by the raw material group of following parts by weight
Into：700 parts of beauveria bassiana, 2 parts of nicotine, 60 parts of diatomite, 7 parts of Metarhizium anisopliae, 20 parts of baicalein, bacteriophagic Bdellovibrio 7
Part, 7 parts of talcum powder, 10 parts of bacillus, 8 parts of vermiculite, 2 parts of sodium carboxymethylcellulose, 4 parts of plant growth regulator, aluminum oxide 8
Part, 5 parts of baicalein, 1.2 parts of garlic oil, 4 parts of matrine, 3 parts of starch, 25 parts of round onions, 3 parts of oxymycin, 2 parts of streptomysin and oneself
15 parts, it is 18 parts of chrysanthemum height, 10 parts of Phytolacca acinosa, 15 parts of the rhizome of Chinese monkshood, 22 parts of the coptis, 15 parts of cordate houttuynia, 4 parts of camphor, 4 parts of folium artemisiae argyi, pale reddish brown
2 parts of fourth, 8 parts of trace element compound.
As the further scheme of the present invention：The bacillus is bacillus subtilis, bafillus natto, lichens bud
Spore bacillus or wax bacillus.
As the further scheme of the present invention：The plant growth regulator is heteroauxin, 2,4-D, Yield-increasing Hormone, naphthalene second
Acid, sevin, gibberellin, zeatin, kinetin, di- phenylurea, triacontanol, brassin lactones, cycocel, sodium humate,
At least one of alar-85 or paclobutrazol plant growth regulator.
As the further scheme of the present invention：The trace element compound is borax, zinc sulfate, ferrous sulfate, sulfuric acid
At least one of copper, manganese sulfate, ammonium molybdate, cobalt oxide, titanium oxide, selenium oxide or KI compound.
The preparation method of the beauveria bassiana microbial pesticide, is comprised the following steps that：
（1）The solid medium of strain is prepared, sterile working is strictly carried out after sterilizing, flat board, 30 DEG C of temperature are forwarded to from inclined-plane
Lower culture 1-3d；By the flat board grown difference picking beauveria bassiana, Metarhizium anisopliae, bacteriophagic Bdellovibrio, bacillus
Single bacterium colony is forwarded in the 50mL conical flasks equipped with fluid nutrient medium, sterile working, 30 DEG C, 130r/min cultures 15-25h；Will
Cultured strain is inoculated in the 500mL conical flasks for filling fluid nutrient medium, sterile working, 30 DEG C, 130r/min cultures 15-
25h；Above-mentioned four kinds of strains are mixed by the parts by weight of the strain, mixed bacterium solution is inoculated in and fills fermentation medium
In fermentation tank, pH6.0-8.0, inoculum concentration 1-5%, 4-7d is cultivated at 30 DEG C, bacterium number in zymotic fluid is reached 8-10 × 108Individual/
ML, obtain mixed bacteria liquid；
（2）Round onions and oneself, chrysanthemum height, Phytolacca acinosa, the rhizome of Chinese monkshood, the coptis, cordate houttuynia, camphor, folium artemisiae argyi, Chinese violet are put into container,
Add 4-6 times of decocting and boil 3-5h, filtered to get filtrate after decoction；
（3）After vermiculite, diatomite, talcum powder are mixed, add 3-5 times of water and continue to be well mixed, carry out after super 3-7min again
Filtered and done, the ultrasonic frequency is 80-100KHZ, and dry temperature is 100-200 DEG C, time 1-3h, is obtained
First solid；Wherein, the vermiculite, diatomite, talcum powder, boehmite are with dry basis；
（4）First solid is subjected to roasting 2-4h at 380-420 DEG C, obtains the second solid；
（5）20-100 mesh sieves are crossed after second solid is crushed, obtain the first powder；By first powder and cigarette
Alkali, baicalein, sodium carboxymethylcellulose, plant growth regulator, aluminum oxide, baicalein, garlic oil, matrine, starch, oxygen are mould
Element, streptomysin, trace element compound and above-mentioned steps（1）-（2）Obtained mixed bacteria liquid, filtrate are well mixed, and obtain
Beauveria bassiana microbial pesticide.
As further scheme of the invention：The composition of the fermentation medium is glucose 60-80g/L, sucrose 20-
40g/L, milk casein peptone 2-4g/L, corn steep liquor 1-3g/L, dipotassium hydrogen phosphate 0.3-0.7g/L, ammonium sulfate 0.01-0.05g/L,
Magnesium sulfate 0.05-0.1g/L, calcium carbonate 0.08-0.12g/L, brown sugar 0.3-0.7g/L, bean sprout juice 2-4g/L, remaining is distillation
Water.
Compared with prior art, the beneficial effects of the invention are as follows：
The raw materials used proportioning of agricultural chemicals of the present invention is simple, and green, cost is cheap；The immunity energy of plant can be improved
Power, it will not remain and accumulate on agricultural product, human living space and health will not be damaged, will not band to ecological environment
To pollute, and active ingredient complexity is various, especially with two or more plants compound preparation when, composition is even more a variety of more
Sample so that insect can not develop immunity to drugs to one or more of compositions therein, so as to receive preferable prevention effect, make work
Skill is simply, conveniently.
Embodiment
The technical scheme of this patent is described in more detail with reference to embodiment.
Embodiment 1
A kind of beauveria bassiana microbial pesticide, is made up of the raw material of following parts by weight：600 parts of beauveria bassiana, 1 part of nicotine,
50 parts of diatomite, 6 parts of Metarhizium anisopliae, 15 parts of baicalein, 6 parts of bacteriophagic Bdellovibrio, 6 parts of talcum powder, 8 parts of bacillus, leech
5 parts of stone, 1 part of sodium carboxymethylcellulose, 3 parts of plant growth regulator, 5 parts of aluminum oxide, 4 parts of baicalein, 0.5 part of garlic oil, hardship
Join 2 parts of alkali, 1 part of starch, 20 parts of round onions, 2 parts of oxymycin, 1 part of streptomysin and oneself 10 parts, 15 parts of chrysanthemum height, 8 parts of Phytolacca acinosa, the rhizome of Chinese monkshood
10 parts, 20 parts of the coptis, 10 parts of cordate houttuynia, 3 parts of camphor, 3 parts of folium artemisiae argyi, 1 part of Chinese violet, 5 parts of trace element compound.
The preparation method of the beauveria bassiana microbial pesticide, is comprised the following steps that：
（1）The solid medium of strain is prepared, sterile working is strictly carried out after sterilizing, flat board, 30 DEG C of temperature are forwarded to from inclined-plane
Lower culture 1d；By the flat board grown difference picking beauveria bassiana, Metarhizium anisopliae, bacteriophagic Bdellovibrio, bacillus list
Bacterium colony is forwarded in the 50mL conical flasks equipped with fluid nutrient medium, sterile working, 30 DEG C, 130r/min cultures 15h；It will cultivate
Strain be inoculated in the 500mL conical flasks for filling fluid nutrient medium, sterile working, 30 DEG C, 130r/min culture 15h；Will be upper
State four kinds of strains to mix by the parts by weight of the strain, mixed bacterium solution is inoculated in the fermentation tank for filling fermentation medium
In, pH6.0, inoculum concentration 1%, 4d is cultivated at 30 DEG C, bacterium number in zymotic fluid is reached 8 × 108Individual/mL, obtains mixed bacteria liquid；
The composition of the fermentation medium is glucose 60g/L, sucrose 20g/L, milk casein peptone 2g/L, corn steep liquor 1g/L, phosphoric acid hydrogen
Dipotassium 0.3g/L, ammonium sulfate 0.01g/L, magnesium sulfate 0.05g/L, calcium carbonate 0.08g/L, brown sugar 0.3g/L, bean sprout juice 2g/L, its
Remaining is distilled water；
（2）Round onions and oneself, chrysanthemum height, Phytolacca acinosa, the rhizome of Chinese monkshood, the coptis, cordate houttuynia, camphor, folium artemisiae argyi, Chinese violet are put into container,
Add 4 times of decocting and boil 3h, filtered to get filtrate after decoction；
（3）After vermiculite, diatomite, talcum powder are mixed, add 3 times of water and continue to be well mixed, carried out again after carrying out super 3min
Filter and dry, the ultrasonic frequency is 80KHZ, and dry temperature is 100 DEG C, time 1h, obtains the first solid；Wherein,
The vermiculite, diatomite, talcum powder, boehmite are with dry basis；
（4）First solid is subjected to roasting 2h at 380 DEG C, obtains the second solid；
（5）20 mesh sieves are crossed after second solid is crushed, obtain the first powder；By first powder and nicotine, Huang
A kind of reed mentioned in ancient books element, sodium carboxymethylcellulose, plant growth regulator, aluminum oxide, baicalein, garlic oil, matrine, starch, oxymycin, chain
Mycin, trace element compound and above-mentioned steps（1）-（2）Obtained mixed bacteria liquid, filtrate are well mixed, and it is white to obtain ball spore
Stiff bacteria microorganism agricultural chemicals.
Embodiment 2
A kind of beauveria bassiana microbial pesticide, is made up of the raw material of following parts by weight：650 parts of beauveria bassiana, nicotine 1.5
Part, 55 parts of diatomite, 6.5 parts of Metarhizium anisopliae, 18 parts of baicalein, 6.5 parts of bacteriophagic Bdellovibrio, 6.5 parts of talcum powder, gemma bar
9 parts of bacterium, 6 parts of vermiculite, 1.5 parts of sodium carboxymethylcellulose, 3.5 parts of plant growth regulator, 6 parts of aluminum oxide, 4.5 parts of baicalein,
1 part of garlic oil, 3 parts of matrine, 2 parts of starch, 22 parts of round onions, 2.5 parts of oxymycin, 1.5 parts of streptomysin and oneself 12 parts, chrysanthemum height
16 parts, 9 parts of Phytolacca acinosa, 12 parts of the rhizome of Chinese monkshood, 21 parts of the coptis, 12 parts of cordate houttuynia, 3.5 parts of camphor, 3.5 parts of folium artemisiae argyi, 1.5 parts of Chinese violet,
6 parts of trace element compound.
The preparation method of the beauveria bassiana microbial pesticide, is comprised the following steps that：
（1）The solid medium of strain is prepared, sterile working is strictly carried out after sterilizing, flat board, 30 DEG C of temperature are forwarded to from inclined-plane
Lower culture 1.5d；By the flat board grown difference picking beauveria bassiana, Metarhizium anisopliae, bacteriophagic Bdellovibrio, bacillus
Single bacterium colony is forwarded in the 50mL conical flasks equipped with fluid nutrient medium, sterile working, 30 DEG C, 130r/min cultures 18h；Will culture
Good strain is inoculated in the 500mL conical flasks for filling fluid nutrient medium, sterile working, 30 DEG C, 130r/min cultures 18h；Will
Above-mentioned four kinds of strains are mixed by the parts by weight of the strain, and mixed bacterium solution is inoculated in the fermentation tank for filling fermentation medium
In, pH6.5, inoculum concentration 2%, 5d is cultivated at 30 DEG C, bacterium number in zymotic fluid is reached 8.5 × 108Individual/mL, obtains Mixed Microbes
Liquid；The composition of the fermentation medium be glucose 65g/L, sucrose 25g/L, milk casein peptone 2.5g/L, corn steep liquor 1.5g/L,
Dipotassium hydrogen phosphate 0.4g/L, ammonium sulfate 0.02g/L, magnesium sulfate 0.06g/L, calcium carbonate 0.09g/L, brown sugar 0.4g/L, bean sprout juice
2.5g/L, remaining is distilled water；
（2）Round onions and oneself, chrysanthemum height, Phytolacca acinosa, the rhizome of Chinese monkshood, the coptis, cordate houttuynia, camphor, folium artemisiae argyi, Chinese violet are put into container,
Add 4.5 times of decocting and boil 3.5h, filtered to get filtrate after decoction；
（3）After vermiculite, diatomite, talcum powder are mixed, add 3.5 times of water and continue to be well mixed, enter again after carrying out super 4min
Row is filtered and done, and the ultrasonic frequency is 85KHZ, and dry temperature is 120 DEG C, time 1.5h, obtains the first solid；Its
In, the vermiculite, diatomite, talcum powder, boehmite are with dry basis；
（4）First solid is subjected to roasting 2.5h at 390 DEG C, obtains the second solid；
（5）40 mesh sieves are crossed after second solid is crushed, obtain the first powder；By first powder and nicotine, Huang
A kind of reed mentioned in ancient books element, sodium carboxymethylcellulose, plant growth regulator, aluminum oxide, baicalein, garlic oil, matrine, starch, oxymycin, chain
Mycin, trace element compound and above-mentioned steps（1）-（2）Obtained mixed bacteria liquid, filtrate are well mixed, and it is white to obtain ball spore
Stiff bacteria microorganism agricultural chemicals.
Embodiment 3
A kind of beauveria bassiana microbial pesticide, is made up of the raw material of following parts by weight：700 parts of beauveria bassiana, 2 parts of nicotine,
60 parts of diatomite, 7 parts of Metarhizium anisopliae, 20 parts of baicalein, 7 parts of bacteriophagic Bdellovibrio, 7 parts of talcum powder, 10 parts of bacillus, leech
8 parts of stone, 2 parts of sodium carboxymethylcellulose, 4 parts of plant growth regulator, 8 parts of aluminum oxide, 5 parts of baicalein, 1.2 parts of garlic oil, hardship
Join 4 parts of alkali, 3 parts of starch, 25 parts of round onions, 3 parts of oxymycin, 2 parts of streptomysin and oneself 15 parts, 18 parts of chrysanthemum height, 10 parts of Phytolacca acinosa, crow
First 15 parts, 22 parts of the coptis, 15 parts of cordate houttuynia, 4 parts of camphor, 4 parts of folium artemisiae argyi, 2 parts of Chinese violet, 8 parts of trace element compound.
The preparation method of the beauveria bassiana microbial pesticide, is comprised the following steps that：
（1）The solid medium of strain is prepared, sterile working is strictly carried out after sterilizing, flat board, 30 DEG C of temperature are forwarded to from inclined-plane
Lower culture 2d；By the flat board grown difference picking beauveria bassiana, Metarhizium anisopliae, bacteriophagic Bdellovibrio, bacillus list
Bacterium colony is forwarded in the 50mL conical flasks equipped with fluid nutrient medium, sterile working, 30 DEG C, 130r/min cultures 20h；It will cultivate
Strain be inoculated in the 500mL conical flasks for filling fluid nutrient medium, sterile working, 30 DEG C, 130r/min culture 20h；Will be upper
State four kinds of strains to mix by the parts by weight of the strain, mixed bacterium solution is inoculated in the fermentation tank for filling fermentation medium
In, pH7.0, inoculum concentration 3%, 6d is cultivated at 30 DEG C, bacterium number in zymotic fluid is reached 9 × 108Individual/mL, obtains mixed bacteria liquid；
The composition of the fermentation medium is glucose 70g/L, sucrose 30g/L, milk casein peptone 3g/L, corn steep liquor 2g/L, phosphoric acid hydrogen
Dipotassium 0.5g/L, ammonium sulfate 0.03g/L, magnesium sulfate 0.08g/L, calcium carbonate 0.1g/L, brown sugar 0.5g/L, bean sprout juice 3g/L, its
Remaining is distilled water；
（2）Round onions and oneself, chrysanthemum height, Phytolacca acinosa, the rhizome of Chinese monkshood, the coptis, cordate houttuynia, camphor, folium artemisiae argyi, Chinese violet are put into container,
Add 5 times of decocting and boil 4h, filtered to get filtrate after decoction；
（3）After vermiculite, diatomite, talcum powder are mixed, add 4 times of water and continue to be well mixed, carried out again after carrying out super 5min
Filter and dry, the ultrasonic frequency is 90KHZ, and dry temperature is 150 DEG C, time 2h, obtains the first solid；Wherein,
The vermiculite, diatomite, talcum powder, boehmite are with dry basis；
（4）First solid is subjected to roasting 3h at 400 DEG C, obtains the second solid；
（5）60 mesh sieves are crossed after second solid is crushed, obtain the first powder；By first powder and nicotine, Huang
A kind of reed mentioned in ancient books element, sodium carboxymethylcellulose, plant growth regulator, aluminum oxide, baicalein, garlic oil, matrine, starch, oxymycin, chain
Mycin, trace element compound and above-mentioned steps（1）-（2）Obtained mixed bacteria liquid, filtrate are well mixed, and it is white to obtain ball spore
Stiff bacteria microorganism agricultural chemicals.
Embodiment 4
A kind of beauveria bassiana microbial pesticide, is made up of the raw material of following parts by weight：750 parts of beauveria bassiana, nicotine 2.5
Part, 65 parts of diatomite, 7.5 parts of Metarhizium anisopliae, 22 parts of baicalein, 7.5 parts of bacteriophagic Bdellovibrio, 7.5 parts of talcum powder, gemma bar
11 parts of bacterium, 9 parts of vermiculite, 2.5 parts of sodium carboxymethylcellulose, 4.5 parts of plant growth regulator, 9 parts of aluminum oxide, 5.5 parts of baicalein,
1.5 parts of garlic oil, 5 parts of matrine, 4 parts of starch, 28 parts of round onions, 3.5 parts of oxymycin, 2.5 parts of streptomysin and oneself 18 parts, chrysanthemum
High 19 parts, 11 parts of Phytolacca acinosa, 18 parts of the rhizome of Chinese monkshood, 24 parts of the coptis, 18 parts of cordate houttuynia, 4.5 parts of camphor, 4.5 parts of folium artemisiae argyi, Chinese violet 2.5
Part, 9 parts of trace element compound.
The preparation method of the beauveria bassiana microbial pesticide, is comprised the following steps that：
（1）The solid medium of strain is prepared, sterile working is strictly carried out after sterilizing, flat board, 30 DEG C of temperature are forwarded to from inclined-plane
Lower culture 2.5d；By the flat board grown difference picking beauveria bassiana, Metarhizium anisopliae, bacteriophagic Bdellovibrio, bacillus
Single bacterium colony is forwarded in the 50mL conical flasks equipped with fluid nutrient medium, sterile working, 30 DEG C, 130r/min cultures 22h；Will culture
Good strain is inoculated in the 500mL conical flasks for filling fluid nutrient medium, sterile working, 30 DEG C, 130r/min cultures 22h；Will
Above-mentioned four kinds of strains are mixed by the parts by weight of the strain, and mixed bacterium solution is inoculated in the fermentation tank for filling fermentation medium
In, pH7.5, inoculum concentration 4%, 6d is cultivated at 30 DEG C, bacterium number in zymotic fluid is reached 9.5 × 108Individual/mL, obtains Mixed Microbes
Liquid；The composition of the fermentation medium be glucose 75g/L, sucrose 35g/L, milk casein peptone 3.5g/L, corn steep liquor 2.5g/L,
Dipotassium hydrogen phosphate 0.6g/L, ammonium sulfate 0.04g/L, magnesium sulfate 0.09g/L, calcium carbonate 0.11g/L, brown sugar 0.6g/L, bean sprout juice
3.5g/L, remaining is distilled water；
（2）Round onions and oneself, chrysanthemum height, Phytolacca acinosa, the rhizome of Chinese monkshood, the coptis, cordate houttuynia, camphor, folium artemisiae argyi, Chinese violet are put into container,
Add 5.5 times of decocting and boil 4.5h, filtered to get filtrate after decoction；
（3）After vermiculite, diatomite, talcum powder are mixed, add 4.5 times of water and continue to be well mixed, enter again after carrying out super 6min
Row is filtered and done, and the ultrasonic frequency is 95KHZ, and dry temperature is 180 DEG C, time 2.5h, obtains the first solid；Its
In, the vermiculite, diatomite, talcum powder, boehmite are with dry basis；
（4）First solid is subjected to roasting 3.5h at 410 DEG C, obtains the second solid；
（5）80 mesh sieves are crossed after second solid is crushed, obtain the first powder；By first powder and nicotine, Huang
A kind of reed mentioned in ancient books element, sodium carboxymethylcellulose, plant growth regulator, aluminum oxide, baicalein, garlic oil, matrine, starch, oxymycin, chain
Mycin, trace element compound and above-mentioned steps（1）-（2）Obtained mixed bacteria liquid, filtrate are well mixed, and it is white to obtain ball spore
Stiff bacteria microorganism agricultural chemicals.
Embodiment 5
A kind of beauveria bassiana microbial pesticide, is made up of the raw material of following parts by weight：800 parts of beauveria bassiana, 3 parts of nicotine,
70 parts of diatomite, 8 parts of Metarhizium anisopliae, 25 parts of baicalein, 8 parts of bacteriophagic Bdellovibrio, 8 parts of talcum powder, 12 parts of bacillus, leech
10 parts of stone, 3 parts of sodium carboxymethylcellulose, 5 parts of plant growth regulator, 10 parts of aluminum oxide, 6 parts of baicalein, 2 parts of garlic oil, hardship
Join 6 parts of alkali, 5 parts of starch, 30 parts of round onions, 4 parts of oxymycin, 3 parts of streptomysin and oneself 20 parts, 20 parts of chrysanthemum height, 12 parts of Phytolacca acinosa, crow
First 20 parts, 25 parts of the coptis, 20 parts of cordate houttuynia, 5 parts of camphor, 5 parts of folium artemisiae argyi, 3 parts of Chinese violet, 10 parts of trace element compound.
The preparation method of the beauveria bassiana microbial pesticide, is comprised the following steps that：
（1）The solid medium of strain is prepared, sterile working is strictly carried out after sterilizing, flat board, 30 DEG C of temperature are forwarded to from inclined-plane
Lower culture 3d；By the flat board grown difference picking beauveria bassiana, Metarhizium anisopliae, bacteriophagic Bdellovibrio, bacillus list
Bacterium colony is forwarded in the 50mL conical flasks equipped with fluid nutrient medium, sterile working, 30 DEG C, 130r/min cultures 25h；It will cultivate
Strain be inoculated in the 500mL conical flasks for filling fluid nutrient medium, sterile working, 30 DEG C, 130r/min culture 25h；Will be upper
State four kinds of strains to mix by the parts by weight of the strain, mixed bacterium solution is inoculated in the fermentation tank for filling fermentation medium
In, pH8.0, inoculum concentration 5%, 7d is cultivated at 30 DEG C, bacterium number in zymotic fluid is reached 10 × 108Individual/mL, obtains mixed bacteria liquid；
The composition of the fermentation medium is glucose 80g/L, sucrose 40g/L, milk casein peptone 4g/L, corn steep liquor 3g/L, phosphoric acid hydrogen
Dipotassium 0.7g/L, ammonium sulfate 0.05g/L, magnesium sulfate 0.1g/L, calcium carbonate 0.12g/L, brown sugar 0.7g/L, bean sprout juice 4g/L, its
Remaining is distilled water；
（2）Round onions and oneself, chrysanthemum height, Phytolacca acinosa, the rhizome of Chinese monkshood, the coptis, cordate houttuynia, camphor, folium artemisiae argyi, Chinese violet are put into container,
Add 6 times of decocting and boil 5h, filtered to get filtrate after decoction；
（3）After vermiculite, diatomite, talcum powder are mixed, add 5 times of water and continue to be well mixed, carried out again after carrying out super 7min
Filter and dry, the ultrasonic frequency is 100KHZ, and dry temperature is 200 DEG C, time 3h, obtains the first solid；Wherein,
The vermiculite, diatomite, talcum powder, boehmite are with dry basis；
（4）First solid is subjected to roasting 4h at 420 DEG C, obtains the second solid；
（5）100 mesh sieves are crossed after second solid is crushed, obtain the first powder；By first powder and nicotine, Huang
A kind of reed mentioned in ancient books element, sodium carboxymethylcellulose, plant growth regulator, aluminum oxide, baicalein, garlic oil, matrine, starch, oxymycin, chain
Mycin, trace element compound and above-mentioned steps（1）-（2）Obtained mixed bacteria liquid, filtrate are well mixed, and it is white to obtain ball spore
Stiff bacteria microorganism agricultural chemicals.
The bacillus is bacillus subtilis, bafillus natto, bacillus licheniformis or wax bacillus.Institute
Plant growth regulator is stated as heteroauxin, 2,4-D, Yield-increasing Hormone, methyl α-naphthyl acetate, sevin, gibberellin, zeatin, kinetin, connection
At least one of acardite, triacontanol, brassin lactones, cycocel, sodium humate, alar-85 or paclobutrazol plant growth is adjusted
Save agent.The trace element compound is borax, zinc sulfate, ferrous sulfate, copper sulphate, manganese sulfate, ammonium molybdate, cobalt oxide, oxygen
Change at least one of titanium, selenium oxide or KI compound.
The raw materials used proportioning of agricultural chemicals of the present invention is simple, and green, cost is cheap；The immune of plant can be improved
Ability, it will not remain and accumulate on agricultural product, human living space and health will not be damaged, will not to ecological environment
Bring pollution, and active ingredient complexity is various, especially with two or more plants compound preparation when, composition is even more a variety of more
Sample so that insect can not develop immunity to drugs to one or more of compositions therein, so as to receive preferable prevention effect, make work
Skill is simply, conveniently.
The better embodiment of this patent is explained in detail above, but this patent is not limited to above-mentioned embodiment party
Formula, can also be on the premise of this patent objective not be departed from one skilled in the relevant art's possessed knowledge
Various changes can be made.
Claims (8)
1. a kind of beauveria bassiana microbial pesticide, it is characterised in that be made up of the raw material of following parts by weight：Beauveria bassiana
600-800 parts, nicotine 1-3 parts, diatomite 50-70 parts, Metarhizium anisopliae 6-8 parts, baicalein 15-25 parts, bacteriophagic Bdellovibrio
6-8 parts, talcum powder 6-8 parts, bacillus 8-12 parts, vermiculite 5-10 parts, sodium carboxymethylcellulose 1-3 parts, plant growth regulating
Agent 3-5 parts, aluminum oxide 5-10 parts, baicalein 4-6 parts, garlic oil 0.5-2 parts, matrine 2-6 parts, starch 1-5 parts, round onions 20-
30 parts, oxymycin 2-4 parts, streptomysin 1-3 parts and own 10-20 parts, chrysanthemum height 15-20 parts, Phytolacca acinosa 8-12 parts, rhizome of Chinese monkshood 10-20
Part, coptis 20-25 parts, cordate houttuynia 10-20 parts, camphor 3-5 parts, folium artemisiae argyi 3-5 parts, Chinese violet 1-3 parts, trace element compound
5-10 parts.
2. beauveria bassiana microbial pesticide according to claim 1, it is characterised in that by the raw material group of following parts by weight
Into：Beauveria bassiana 650-750 parts, nicotine 1.5-2.5 parts, diatomite 55-65 parts, Metarhizium anisopliae 6.5-7.5 parts, radix scutellariae
Plain 18-22 parts, bacteriophagic Bdellovibrio 6.5-7.5 parts, talcum powder 6.5-7.5 parts, bacillus 9-11 parts, vermiculite 6-9 parts, carboxymethyl
Sodium cellulosate 1.5-2.5 parts, plant growth regulator 3.5-4.5 parts, aluminum oxide 6-9 parts, baicalein 4.5-5.5 parts, garlic oil
1-1.5 parts, matrine 3-5 parts, starch 2-4 parts, round onions 22-28 parts, oxymycin 2.5-3.5 parts, streptomysin 1.5-2.5 parts and
Own 12-18 parts, chrysanthemum height 16-19 parts, Phytolacca acinosa 9-11 parts, rhizome of Chinese monkshood 12-18 parts, coptis 21-24 parts, cordate houttuynia 12-18 parts, camphor
3.5-4.5 parts, folium artemisiae argyi 3.5-4.5 parts, Chinese violet 1.5-2.5 parts, trace element compound 6-9 parts.
3. beauveria bassiana microbial pesticide according to claim 1, it is characterised in that by the raw material group of following parts by weight
Into：700 parts of beauveria bassiana, 2 parts of nicotine, 60 parts of diatomite, 7 parts of Metarhizium anisopliae, 20 parts of baicalein, bacteriophagic Bdellovibrio 7
Part, 7 parts of talcum powder, 10 parts of bacillus, 8 parts of vermiculite, 2 parts of sodium carboxymethylcellulose, 4 parts of plant growth regulator, aluminum oxide 8
Part, 5 parts of baicalein, 1.2 parts of garlic oil, 4 parts of matrine, 3 parts of starch, 25 parts of round onions, 3 parts of oxymycin, 2 parts of streptomysin and oneself
15 parts, it is 18 parts of chrysanthemum height, 10 parts of Phytolacca acinosa, 15 parts of the rhizome of Chinese monkshood, 22 parts of the coptis, 15 parts of cordate houttuynia, 4 parts of camphor, 4 parts of folium artemisiae argyi, pale reddish brown
2 parts of fourth, 8 parts of trace element compound.
4. according to any described beauveria bassiana microbial pesticides of claim 1-3, it is characterised in that the bacillus is
Bacillus subtilis, bafillus natto, bacillus licheniformis or wax bacillus.
5. according to any described beauveria bassiana microbial pesticides of claim 1-3, it is characterised in that the plant growth is adjusted
Section agent is heteroauxin, 2,4-D, Yield-increasing Hormone, methyl α-naphthyl acetate, sevin, gibberellin, zeatin, kinetin, di- phenylurea, 30
At least one of alkanol, brassin lactones, cycocel, sodium humate, alar-85 or paclobutrazol plant growth regulator.
6. according to any described beauveria bassiana microbial pesticides of claim 1-3, it is characterised in that the trace elementization
Compound is borax, zinc sulfate, ferrous sulfate, copper sulphate, manganese sulfate, ammonium molybdate, cobalt oxide, titanium oxide, selenium oxide or KI
At least one of compound.
A kind of 7. preparation method of beauveria bassiana microbial pesticide as described in claim 1-3 is any, it is characterised in that tool
Body step is as follows：
（1）The solid medium of strain is prepared, sterile working is strictly carried out after sterilizing, flat board, 30 DEG C of temperature are forwarded to from inclined-plane
Lower culture 1-3d；By the flat board grown difference picking beauveria bassiana, Metarhizium anisopliae, bacteriophagic Bdellovibrio, bacillus
Single bacterium colony is forwarded in the 50mL conical flasks equipped with fluid nutrient medium, sterile working, 30 DEG C, 130r/min cultures 15-25h；Will
Cultured strain is inoculated in the 500mL conical flasks for filling fluid nutrient medium, sterile working, 30 DEG C, 130r/min cultures 15-
25h；Above-mentioned four kinds of strains are mixed by the parts by weight of the strain, mixed bacterium solution is inoculated in and fills fermentation medium
In fermentation tank, pH6.0-8.0, inoculum concentration 1-5%, 4-7d is cultivated at 30 DEG C, bacterium number in zymotic fluid is reached 8-10 × 108Individual/
ML, obtain mixed bacteria liquid；
（2）Round onions and oneself, chrysanthemum height, Phytolacca acinosa, the rhizome of Chinese monkshood, the coptis, cordate houttuynia, camphor, folium artemisiae argyi, Chinese violet are put into container,
Add 4-6 times of decocting and boil 3-5h, filtered to get filtrate after decoction；
（3）After vermiculite, diatomite, talcum powder are mixed, add 3-5 times of water and continue to be well mixed, carry out after super 3-7min again
Filtered and done, the ultrasonic frequency is 80-100KHZ, and dry temperature is 100-200 DEG C, time 1-3h, is obtained
First solid；Wherein, the vermiculite, diatomite, talcum powder, boehmite are with dry basis；
（4）First solid is subjected to roasting 2-4h at 380-420 DEG C, obtains the second solid；
（5）20-100 mesh sieves are crossed after second solid is crushed, obtain the first powder；By first powder and cigarette
Alkali, baicalein, sodium carboxymethylcellulose, plant growth regulator, aluminum oxide, baicalein, garlic oil, matrine, starch, oxygen are mould
Element, streptomysin, trace element compound and above-mentioned steps（1）-（2）Obtained mixed bacteria liquid, filtrate are well mixed, and obtain
Beauveria bassiana microbial pesticide.
8. the preparation method of beauveria bassiana microbial pesticide according to claim 7, it is characterised in that the fermentation training
The composition for supporting base is glucose 60-80g/L, sucrose 20-40g/L, milk casein peptone 2-4g/L, corn steep liquor 1-3g/L, phosphoric acid hydrogen
Dipotassium 0.3-0.7g/L, ammonium sulfate 0.01-0.05g/L, magnesium sulfate 0.05-0.1g/L, calcium carbonate 0.08-0.12g/L, brown sugar
0.3-0.7g/L, bean sprout juice 2-4g/L, remaining is distilled water.
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| CN108902184A (en) * | 2018-08-03 | 2018-11-30 | 西北农林科技大学 | Brill moth beetle process for preparing microbial insecticide |
| CN115093294A (en) * | 2022-06-16 | 2022-09-23 | 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) | Microbial pesticide preparation for plant protection and growth regulation |
| CN115530184A (en) * | 2022-10-31 | 2022-12-30 | 重庆市农业科学院 | Medicament for preventing and treating indoor reptiles as well as preparation method and application thereof |
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Application publication date: 20171117 |
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| RJ01 | Rejection of invention patent application after publication |