CN110200028A - A kind of cane planting insecticide and preparation method thereof - Google Patents
A kind of cane planting insecticide and preparation method thereof Download PDFInfo
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- CN110200028A CN110200028A CN201910487213.4A CN201910487213A CN110200028A CN 110200028 A CN110200028 A CN 110200028A CN 201910487213 A CN201910487213 A CN 201910487213A CN 110200028 A CN110200028 A CN 110200028A
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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Abstract
The invention discloses a kind of cane planting insecticides and preparation method thereof.The cane planting is fermented with insecticide by compound bacteria and Chinese herb culture medium, wherein the compound bacteria is selected from one or both of thorn saccharopolyspora strain, aspergillus niger；The Chinese herb culture medium, according to parts by weight, comprising: 20~40 parts of Fructus Forsythiae, 5~15 parts of garlic, 3~12 parts of Celastrus angulatus, 1~8 part of crotons, 10~30 parts of crowndaisy chrysanthemum, 30~50 parts of nutrients, 30~50 parts of deionized water.The present invention is extracted the effective component in Chinese herbal medicine by melanomyces and thorn saccharopolyspora strain, simultaneously, generated cometabolism ingredient acts synergistically with active constituent contained by Chinese herbal medicine during the fermentation for melanomyces and thorn saccharopolyspora strain, and insecticide of the invention is enable to reduce the pest and disease damage of crops to greatest extent.
Description
Technical field
The present invention relates to technical field of pesticide, in particular to a kind of cane planting insecticide and preparation method thereof.
Background technique
Sugarcane is temperate zone and tropical crops, is the raw material of manufacture sucrose, and can refine ethyl alcohol as energy substitution product, but
It is in the planting process of sugarcane, it may occur that pest and disease damage will lead to serious economic loss if do not administered.Sweet
In sugarcane planting process, people are typically employed in field sprinkling chemical pesticide to prevent and treat the generation of sugarcane pest and disease damage.Due to certain agricultures
Medicine toxicity is big, is not easy to decompose, and extended residual is accumulated in the soil, can cause remains of pesticide occur in sugarcane and livestock products,
Endanger the health of the mankind.
Summary of the invention
The purpose of the present invention is to provide a kind of cane planting insecticides and preparation method thereof, are asked with solving above-mentioned technology
Topic.
To achieve the goals above, the technical solution of the present invention is as follows:
In a first aspect, a kind of cane planting insecticide provided by the invention, is fermented by compound bacteria and Chinese herb culture medium
It forms, wherein the compound bacteria is selected from one or both of thorn saccharopolyspora strain, aspergillus niger；The Chinese herb culture medium, by weight
Measure number meter, comprising: 20~40 parts of Fructus Forsythiae, 5~15 parts of garlic, 3~12 parts of Celastrus angulatus, 1~8 part of crotons, 10~30 parts of crowndaisy chrysanthemum,
30~50 parts of nutrients, 30~50 parts of deionized water.
Preferably, the nutrients is selected from one of cornstarch, cotton seed meal, sucrose, glucose, beancake powder or more
Kind.
It is further preferred that the nutrients is 1~3:1~3:1 cornstarch, cotton seed meal and grape by weight ratio
Sugar.
Preferably, the compound bacteria is made of the thorn saccharopolyspora strain and aspergillus niger that weight ratio is 0.1~0.5:1.
Preferably, the Chinese herb culture medium is to be made using following methods:
It crushes, then is poured into pressure cooker after Fructus Forsythiae, garlic, Celastrus angulatus, crotons, crowndaisy chrysanthemum are mixed, is added into pressure cooker
Deionized water is heated to 90~105 DEG C after mixing evenly, keeps the temperature 20~40min under stirring conditions, slurry is made, then
Nutrients is added while hot, stirs evenly and Chinese herb culture medium can be obtained.
Preferably, the Chinese herb culture medium, according to parts by weight, comprising: 24~36 parts of Fructus Forsythiae, 7~11 parts of garlic, hardship
5~9 parts of skin rattan, 3~6 parts of crotons, 14~25 parts of crowndaisy chrysanthemum, 40~50 parts of nutrients, 34~48 parts of deionized water.
Preferably, the weight ratio of the compound bacteria and the Chinese herb culture medium is 1~20%.
It is further preferred that the weight ratio of the compound bacteria and the Chinese herb culture medium is 3~10%.
Second aspect, a kind of preparation method of cane planting insecticide as described in relation to the first aspect provided by the invention,
Include the following steps:
(1) compound bacteria is added in Chinese herb culture medium, is stirred evenly, control fermentation temperature is 25~30 DEG C, aerobic
Fermentation 1~3 day, is adjusted to 30~38 DEG C for fermentation temperature, aerobic fermentation 1~3 day, obtains compound bacteria fermentation material；
(2) it by after the resulting compound bacteria fermentation material sterilizing of step (1), is added in deionized water, 1~3h of ultrasonic extraction, warp
Cane planting insecticide is obtained by filtration.
Preferably, in the step (2) deionized water additive amount be the compound bacteria fermentation material weight 5~15
Times.
Compared with prior art the beneficial effects of the present invention are:
The present invention is extracted the effective component in Chinese herbal medicine by melanomyces and thorn saccharopolyspora strain, meanwhile, melanomyces
And thorn saccharopolyspora strain during the fermentation caused by there is association between active constituent contained by cometabolism ingredient and Chinese herbal medicine
Same synergistic effect enables insecticide of the invention to reduce the pest and disease damage of crops to greatest extent；Experiments have shown that the present invention provides
Cane planting insecticide have good control efficiency to sugarcane moth borer, sugar cane cottony aphid, sugarcane chafer, termite etc..
Specific embodiment
Specific embodiments of the present invention will be further explained below.It should be noted that for these implementations
The explanation of mode is used to help understand the present invention, but and does not constitute a limitation of the invention.In addition, invention described below
Technical characteristic involved in each embodiment can be combined with each other as long as they do not conflict with each other.
Herbal raw material employed in following embodiment is the raw material of fresh clean.
Embodiment 1
A kind of preparation method of cane planting insecticide provided in this embodiment, includes the following steps:
(1) following raw materials according to parts by weight: 24 parts of Fructus Forsythiae, 11 parts of garlic, 9 parts of Celastrus angulatus, 3 parts of crotons, Tong are weighed
14 parts of wormwood artemisia, 15 parts of cornstarch, 15 parts of cotton seed meal, 15 parts of glucose, 48 parts of deionized water, by Fructus Forsythiae, garlic, Celastrus angulatus, bar
It crushes, then is poured into pressure cooker after beans, crowndaisy chrysanthemum mixing, deionized water is added into pressure cooker, is heated to 95 ± 2 after mixing evenly
DEG C, 30min is kept the temperature under stirring conditions, slurry is made, cornstarch, cotton seed meal, glucose are then added while hot, and stirring is equal
It is even that Chinese herb culture medium can be obtained；
(2) Chinese herb culture medium and compound bacteria are added in fermentor, are uniformly mixed, control fermentation temperature is 28
DEG C, it ferments 2 days under conditions of oxygenation and stirring, fermentation temperature is adjusted to 36 DEG C, ferment 3 under conditions of oxygenation and stirring
It, obtains compound bacteria fermentation material, wherein the additive amount of compound bacteria is the 10% of the weight of Chinese herb culture medium, and compound bacteria is by weight
Amount is formed than the thorn saccharopolyspora strain and aspergillus niger for being 0.1:1；
(3) resulting compound bacteria fermentation material is put into autoclave, is heated to 121 DEG C of heat preservations under sealed conditions
Sterilize 30min, the compound bacteria fermentation material after sterilizing is mixed with deionized water according to the weight ratio of 1:10, ultrasonic extraction 3h, passes through
Cane planting insecticide is obtained by filtration.
Embodiment 2
A kind of preparation method of cane planting insecticide provided in this embodiment, includes the following steps:
(1) following raw materials according to parts by weight: 36 parts of Fructus Forsythiae, 7 parts of garlic, 5 parts of Celastrus angulatus, 6 parts of crotons, crowndaisy chrysanthemum are weighed
25 parts, 18 parts of cornstarch, 18 parts of cotton seed meal, 6 parts of glucose, 34 parts of deionized water, by Fructus Forsythiae, garlic, Celastrus angulatus, crotons,
It crushes, then is poured into pressure cooker after crowndaisy chrysanthemum mixing, deionized water is added into pressure cooker, is heated to 95 ± 2 DEG C after mixing evenly,
30min is kept the temperature under stirring conditions, slurry is made, and cornstarch, cotton seed meal, glucose are then added while hot, stirs evenly
Chinese herb culture medium can be obtained；
(2) Chinese herb culture medium and compound bacteria are added in fermentor, are uniformly mixed, control fermentation temperature is 25
DEG C, it ferments 1 day under conditions of oxygenation and stirring, fermentation temperature is adjusted to 38 DEG C, ferment 3 under conditions of oxygenation and stirring
It, obtains compound bacteria fermentation material, wherein the additive amount of compound bacteria is the 3% of the weight of Chinese herb culture medium, and compound bacteria is by weight
Than thorn saccharopolyspora strain and the aspergillus niger composition for 0.5:1；
(3) resulting compound bacteria fermentation material is put into autoclave, is heated to 121 DEG C of heat preservations under sealed conditions
Sterilize 30min, the compound bacteria fermentation material after sterilizing is mixed with deionized water according to the weight ratio of 1:10, ultrasonic extraction 2h, passes through
Cane planting insecticide is obtained by filtration.
Embodiment 3
A kind of preparation method of cane planting insecticide provided in this embodiment, includes the following steps:
(1) following raw materials according to parts by weight: 30 parts of Fructus Forsythiae, 9 parts of garlic, 7 parts of Celastrus angulatus, 5 parts of crotons, crowndaisy chrysanthemum are weighed
20 parts, 15 parts of cornstarch, 20 parts of cotton seed meal, 10 parts of glucose, 45 parts of deionized water, by Fructus Forsythiae, garlic, Celastrus angulatus, crotons,
It crushes, then is poured into pressure cooker after crowndaisy chrysanthemum mixing, deionized water is added into pressure cooker, is heated to 95 ± 2 DEG C after mixing evenly,
30min is kept the temperature under stirring conditions, slurry is made, and cornstarch, cotton seed meal, glucose are then added while hot, stirs evenly
Chinese herb culture medium can be obtained；
(2) Chinese herb culture medium and compound bacteria are added in fermentor, are uniformly mixed, control fermentation temperature is 27
DEG C, it ferments 2 days under conditions of oxygenation and stirring, fermentation temperature is adjusted to 37 DEG C, ferment 3 under conditions of oxygenation and stirring
It, obtains compound bacteria fermentation material, wherein the additive amount of compound bacteria is the 8% of the weight of Chinese herb culture medium, and compound bacteria is by weight
Than thorn saccharopolyspora strain and the aspergillus niger composition for 0.3:1；
(3) resulting compound bacteria fermentation material is put into autoclave, is heated to 121 DEG C of heat preservations under sealed conditions
Sterilize 30min, the compound bacteria fermentation material after sterilizing is mixed with deionized water according to the weight ratio of 1:10, ultrasonic extraction 2h, passes through
Cane planting insecticide is obtained by filtration.
Embodiment 4
A kind of preparation method of cane planting insecticide provided in this embodiment, includes the following steps:
(1) following raw materials according to parts by weight: 28 parts of Fructus Forsythiae, 10 parts of garlic, 6 parts of Celastrus angulatus, 5 parts of crotons, Tong are weighed
21 parts of wormwood artemisia, 20 parts of cornstarch, 20 parts of cotton seed meal, 10 parts of glucose, 45 parts of deionized water, by Fructus Forsythiae, garlic, Celastrus angulatus, bar
It crushes, then is poured into pressure cooker after beans, crowndaisy chrysanthemum mixing, deionized water is added into pressure cooker, is heated to 95 ± 2 after mixing evenly
DEG C, 30min is kept the temperature under stirring conditions, slurry is made, cornstarch, cotton seed meal, glucose are then added while hot, and stirring is equal
It is even that Chinese herb culture medium can be obtained；
(2) Chinese herb culture medium and compound bacteria are added in fermentor, are uniformly mixed, control fermentation temperature is 25
DEG C, it ferments 2 days under conditions of oxygenation and stirring, fermentation temperature is adjusted to 35 DEG C, ferment 2 under conditions of oxygenation and stirring
It, obtains compound bacteria fermentation material, wherein the additive amount of compound bacteria is the 5% of the weight of Chinese herb culture medium, and compound bacteria is by weight
Than thorn saccharopolyspora strain and the aspergillus niger composition for 0.4:1；
(3) resulting compound bacteria fermentation material is put into autoclave, is heated to 121 DEG C of heat preservations under sealed conditions
Sterilize 30min, the compound bacteria fermentation material after sterilizing is mixed with deionized water according to the weight ratio of 1:10, ultrasonic extraction 2h, passes through
Cane planting insecticide is obtained by filtration.
Embodiment 5
A kind of preparation method of cane planting insecticide provided in this embodiment, includes the following steps:
(1) following raw materials according to parts by weight: 20 parts of Fructus Forsythiae, 5 parts of garlic, 3 parts of Celastrus angulatus, 1 part of crotons, crowndaisy chrysanthemum are weighed
10 parts, 20 parts of cornstarch, 20 parts of cotton seed meal, 10 parts of glucose, 30 parts of deionized water, by Fructus Forsythiae, garlic, Celastrus angulatus, crotons,
It crushes, then is poured into pressure cooker after crowndaisy chrysanthemum mixing, deionized water is added into pressure cooker, is heated to 90 DEG C after mixing evenly,
20min is kept the temperature under conditions of stirring, slurry is made, and cornstarch, cotton seed meal, glucose are then added while hot, stirs evenly i.e.
Chinese herb culture medium can be obtained；
(2) Chinese herb culture medium and compound bacteria are added in fermentor, are uniformly mixed, control fermentation temperature is 25
DEG C, it ferments 1 day under conditions of oxygenation and stirring, fermentation temperature is adjusted to 38 DEG C, ferment 3 under conditions of oxygenation and stirring
It, obtains compound bacteria fermentation material, wherein the additive amount of compound bacteria is the 5% of the weight of Chinese herb culture medium, and compound bacteria is by weight
Than thorn saccharopolyspora strain and the aspergillus niger composition for 0.2:1；
(3) resulting compound bacteria fermentation material is put into autoclave, is heated to 121 DEG C of heat preservations under sealed conditions
Sterilize 30min, the compound bacteria fermentation material after sterilizing is mixed with deionized water according to the weight ratio of 1:10, ultrasonic extraction 1h, passes through
Cane planting insecticide is obtained by filtration.
Embodiment 6
A kind of preparation method of cane planting insecticide provided in this embodiment, includes the following steps:
(1) following raw materials according to parts by weight: 40 parts of Fructus Forsythiae, 15 parts of garlic, 12 parts of Celastrus angulatus, 8 parts of crotons, Tong are weighed
30 parts of wormwood artemisia, 10 parts of cornstarch, 10 parts of cotton seed meal, 10 parts of glucose, 50 parts of deionized water, by Fructus Forsythiae, garlic, Celastrus angulatus, bar
It crushes, then is poured into pressure cooker after beans, crowndaisy chrysanthemum mixing, deionized water is added into pressure cooker, is heated to 100 after mixing evenly
DEG C, 30min is kept the temperature under stirring conditions, slurry is made, cornstarch, cotton seed meal, glucose are then added while hot, and stirring is equal
It is even that Chinese herb culture medium can be obtained；
(2) Chinese herb culture medium and compound bacteria are added in fermentor, are uniformly mixed, control fermentation temperature is 30
DEG C, it ferments 1 day under conditions of oxygenation and stirring, fermentation temperature is adjusted to 32 DEG C, ferment 3 under conditions of oxygenation and stirring
It, obtains compound bacteria fermentation material, wherein the additive amount of compound bacteria is the 5% of the weight of Chinese herb culture medium, and compound bacteria is by weight
Than thorn saccharopolyspora strain and the aspergillus niger composition for 0.2:1；
(3) resulting compound bacteria fermentation material is put into autoclave, is heated to 121 DEG C of heat preservations under sealed conditions
Sterilize 30min, the compound bacteria fermentation material after sterilizing is mixed with deionized water according to the weight ratio of 1:10, ultrasonic extraction 3h, passes through
Cane planting insecticide is obtained by filtration.
Comparative example 1
(1) following raw materials according to parts by weight: 24 parts of Fructus Forsythiae, 11 parts of garlic, 9 parts of Celastrus angulatus, 3 parts of crotons, Tong are weighed
14 parts of wormwood artemisia, 15 parts of cornstarch, 15 parts of cotton seed meal, 15 parts of glucose, 48 parts of deionized water, by Fructus Forsythiae, garlic, Celastrus angulatus, bar
It crushes, then is poured into pressure cooker after beans, crowndaisy chrysanthemum mixing, deionized water is added into pressure cooker, is heated to 95 ± 2 after mixing evenly
DEG C, 30min is kept the temperature under stirring conditions, slurry is made, cornstarch, cotton seed meal, glucose are then added while hot, and stirring is equal
It is even that Chinese herb culture medium can be obtained；
(2) Chinese herb culture medium is put into autoclave, is heated to 121 DEG C of insulated sterilizings under sealed conditions
30min mixes the Chinese herb culture medium after sterilizing with deionized water, ultrasonic extraction 3h, through filtering according to the weight ratio of 1:10
Obtain insecticide.
Comparative example 2
(1) following raw materials according to parts by weight: 24 parts of Fructus Forsythiae, 11 parts of garlic, 9 parts of Celastrus angulatus, 3 parts of crotons, Tong are weighed
14 parts of wormwood artemisia, 15 parts of cornstarch, 15 parts of cotton seed meal, 15 parts of glucose, 48 parts of deionized water, by Fructus Forsythiae, garlic, Celastrus angulatus, bar
It crushes, then is poured into pressure cooker after beans, crowndaisy chrysanthemum mixing, deionized water is added into pressure cooker, is heated to 95 ± 2 after mixing evenly
DEG C, 30min is kept the temperature under stirring conditions, slurry is made, cornstarch, cotton seed meal, glucose are then added while hot, and stirring is equal
It is even that Chinese herb culture medium can be obtained；
(2) Chinese herb culture medium and aspergillus niger are added in fermentor, are uniformly mixed, control fermentation temperature is 28
DEG C, it ferments 2 days under conditions of oxygenation and stirring, fermentation temperature is adjusted to 36 DEG C, ferment 3 under conditions of oxygenation and stirring
It, obtains fermentation of Aspergillus niger object, wherein the additive amount of aspergillus niger is 10%；
(3) resulting fermentation of Aspergillus niger object is put into autoclave, is heated to 121 DEG C of heat preservations under sealed conditions
Sterilize 30min, the fermentation material after sterilizing is mixed with deionized water according to the weight ratio of 1:10, ultrasonic extraction 3h, through filtering
To cane planting insecticide.
Effect example
It is 10m that 8 pieces of areas are chosen in a cane -growing region in Guangxi2And sugarcane field similar in insect pest, and build modeling
Expect greenhouse, 8 pieces of sugarcane fields are separated, spray the insecticide of Examples 1 to 6 and comparative example 1~2 respectively, the amount of spraying is
200mL/m2, after spraying 3 days, detection kill sugarcane moth borer, sugarcane aphid quantity, and calculate separately 8 pieces ground sugarcane moth borers and
The deinsectization rate of sugarcane aphid:
1, the The dead quantity/8 piece ground sugarcane moth borer of the ground of the deinsectization rate (%) of sugarcane moth borer=a certain piece sugarcane moth borer is dead
Die sum × 100%；
2, the The dead quantity/8 piece ground sugarcane aphid of the ground of the deinsectization rate (%) of sugarcane aphid=a certain piece sugarcane aphid is dead
Die sum × 100%；
Statistical result is as shown in table 1.
1 deinsectization effect of table
It can be seen from the results above that effective component of the Chinese herbal medicine extract as deinsectization, comparison is used alone in comparative example 1
Example 2 uses active constituent contained by the secondary metabolite and Chinese herbal medicine of fermentation of Aspergillus niger to compare as the effective component of deinsectization
Have very in the insecticide of comparative example 1 and comparative example 2, the embodiment of the present invention 1~6 to sugarcane moth borer, the sugarcane aphid of sugarcane garden
It is good to go out except effect, melanomyces and thorn saccharopolyspora strain during the fermentation caused by cometabolism ingredient with contained by Chinese herbal medicine
There is significant synergistic effect between active constituent, wherein the deinsectization effect of the resulting insecticide of Examples 1 to 4 is more preferable.
Above the embodiments of the present invention are described in detail, but the present invention is not limited to described embodiments.It is right
For those skilled in the art, in the case where not departing from the principle of the invention and spirit, these embodiments are carried out more
Kind change, modification, replacement and modification, still fall in protection scope of the present invention.
Claims (10)
1. a kind of cane planting insecticide, which is characterized in that fermented by compound bacteria and Chinese herb culture medium, wherein institute
It states compound bacteria and is selected from one or both of thorn saccharopolyspora strain, aspergillus niger；The Chinese herb culture medium, according to parts by weight, packet
It includes: 20~40 parts of Fructus Forsythiae, 5~15 parts of garlic, 3~12 parts of Celastrus angulatus, 1~8 part of crotons, 10~30 parts of crowndaisy chrysanthemum, nutrients 30~
50 parts, 30~50 parts of deionized water.
2. cane planting insecticide according to claim 1, which is characterized in that the nutrients be selected from cornstarch,
One of cotton seed meal, sucrose, glucose, beancake powder are a variety of.
3. cane planting insecticide according to claim 2, which is characterized in that the nutrients by weight ratio be 1~
Cornstarch, cotton seed meal and the glucose of 3:1~3:1.
4. cane planting insecticide according to claim 1, which is characterized in that the compound bacteria is 0.1 by weight ratio
The thorn saccharopolyspora strain and aspergillus niger of~0.5:1 forms.
5. cane planting insecticide according to claim 1, which is characterized in that the Chinese herb culture medium is under using
Method is stated to be made:
Fructus Forsythiae, garlic, Celastrus angulatus, crotons, crowndaisy chrysanthemum be crushed after mixing, then poured into pressure cooker, be added into pressure cooker go from
Sub- water is heated to 90~105 DEG C after mixing evenly, keeps the temperature 20~40min under stirring conditions, slurry is made, then while hot
Nutrients is added, stirs evenly and Chinese herb culture medium can be obtained.
6. cane planting insecticide according to claim 1, which is characterized in that the Chinese herb culture medium, by weight
Number meter, comprising: 24~36 parts of Fructus Forsythiae, 7~11 parts of garlic, 5~9 parts of Celastrus angulatus, 3~6 parts of crotons, 14~25 parts of crowndaisy chrysanthemum, battalion
Support 40~50 parts of object, 34~48 parts of deionized water.
7. cane planting insecticide according to claim 1, which is characterized in that the compound bacteria and the Chinese herbal medicine are trained
The weight ratio for supporting base is 1~20%.
8. cane planting insecticide according to claim 7, which is characterized in that the compound bacteria and the Chinese herbal medicine are trained
The weight ratio for supporting base is 3~10%.
9. a kind of preparation method of cane planting insecticide as described in any one of claims 1 to 8, which is characterized in that packet
Include following steps:
(1) compound bacteria is added in Chinese herb culture medium, is stirred evenly, control fermentation temperature is 25~30 DEG C, aerobic fermentation 1
~3 days, fermentation temperature is adjusted to 30~38 DEG C, aerobic fermentation 1~3 day, obtains compound bacteria fermentation material；
(2) it by after the resulting compound bacteria fermentation material sterilizing of step (1), is added in deionized water, 1~3h of ultrasonic extraction, through filtering
Obtain cane planting insecticide.
10. the preparation method of cane planting insecticide according to claim 9, which is characterized in that in the step (2)
The additive amount of deionized water is 5~15 times of the weight of the compound bacteria fermentation material.
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