CN105794845A - Microbial pesticide and preparing method thereof - Google Patents
Microbial pesticide and preparing method thereof Download PDFInfo
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- CN105794845A CN105794845A CN201610237627.8A CN201610237627A CN105794845A CN 105794845 A CN105794845 A CN 105794845A CN 201610237627 A CN201610237627 A CN 201610237627A CN 105794845 A CN105794845 A CN 105794845A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
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- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention relates to microbial pesticide and a preparing method thereof.The microbial pesticide is prepared from vermiculite, kieselguhr, talcum powder, aluminum oxide, bacillus hypha, sodium alga acid, sodium carboxymethyl cellulose and chitin.The dry basis weight ratio of vermiculite to kieselguhr to talcum powder to aluminum oxide is 1:(3-10):(15-35):(0.1-0.6):(0.1-0.6).The weight fraction of sodium alga acid in the microbial pesticide is 0.1-3%, the weight fraction of sodium carboxymethyl cellulose is 0.1-3%, the weight fraction of chitin is 0.1-3%, and the inoculation bacterial colony of bacillus is 109-1010 cfu/g.The microbial pesticide is good in effect and high in release rate.
Description
Technical field
The present invention relates to pesticide field, be specifically related to a kind of microbial pesticide and preparation method thereof.
Background technology
Microbial pesticide (microbial pesticide) includes agricultural antibiotic and alive microbial agrochemical.Disease, worm, grass, the plague of rats and the promotion plant growth harmed the crops for utilizing microorganism or its metabolite to prevent and treat.It include with bacterium control worm, fungus treatment, with bacterium weeding etc..It is strong that this kind of pesticide has selectivity, to people, animal, crops and natural environment safety, does not injure natural enemy, is not likely to produce the features such as resistance.These microbial pesticides include antibacterial, fungus, virus or its metabolite, such as Bacillus thuringiensis, muscardine, nucleopolyhedrosis virus, jingganmycin, c-type bacillus botulinus extracellular toxin etc..Along with the requirement that people are the highest to environmental conservation, one of developing direction of microbial pesticide pesticide the most from now on.
Chinese patent CN105010438A discloses and the present invention relates to a kind of microbial pesticide, it is made up of the raw material of following weight portion: kill aphid element powder 10-20 part, baicalin 1-5 part, Oleum Bulbus Allii 0.5-2 part, matrine 2-6 part, starch 1-5 part, onion 4-30 part, escherichia coli powder 1-15 part, wheel branch cryptogam end 2-6 part.This invention provides a kind of microbial pesticide, and with microorganism powder and plant material as major ingredient, raw material is easy to get, cheap, and raw materials used proportioning is simple, and environmental protection is with low cost.
Chinese patent CN102381912A discloses a kind of microbial pesticide preparation for plant protection growth regulating; including Wuyi bacterium spore powder 0.5-5%, choline chloride 5-25%, light rare earth 10-30%, methionine 5-20%, zinc sulfate 1-8%, copper sulfate 0.3-3%, boric acid 1-5%, vitamin B 0.05-0.5%, vitamin C 0.05-0.5%, inserts Kaolin surplus；The advantage of this invention is: based on Wuyi biocontrol microorganisms spore, the organic acid such as the salicylic acid simultaneously coordinating plant-less toxicity and other be conducive to the composition such as choline chloride, glycine betaine, light rare earth, aminoacid etc. of plant stress-resistance growth promoting, have obvious diseases prevention, disease-resistant, degeneration-resistant, improve crop yield, improve crop products quality, and effect of increasing production is obvious.
Existing microbial pesticide release rate is relatively low, easily causes the problems such as efficiency is low.
Summary of the invention
In order to solve the problems referred to above that prior art exists, the invention provides a kind of microbial pesticide and preparation method thereof, the microbial pesticide of the present invention is the most effective, and release rate is high.
The technical solution adopted in the present invention is:
The present invention provides a kind of microbial pesticide, it is characterized in that, this microbial pesticide includes Vermiculitum, kieselguhr, Pulvis Talci, aluminium oxide, bacillus cereus mycelia, sodium alginate, sodium carboxymethyl cellulose and chitin, wherein, described Vermiculitum, kieselguhr, the butt weight ratio of Pulvis Talci and aluminium oxide is 1:(3-10): (15-35): (0.1-0.6): (0.1-0.6), in described microbial pesticide, the weight fraction of sodium alginate is 0.1-3 weight %, the weight fraction of sodium carboxymethyl cellulose is 0.1-3 weight %, the weight fraction of chitin is 0.1-3 weight %, the inoculation bacterium colony of bacillus cereus is 109-1010cfu/g。
Preferably, the butt weight ratio of described Vermiculitum, kieselguhr, Pulvis Talci and aluminium oxide is 1:(5-7): (25-30): (0.2-0.4): (0.2-0.4), in described microbial pesticide, the weight fraction of sodium alginate is 0.5-1 weight %, the weight fraction of sodium carboxymethyl cellulose is 0.5-1 weight %, and the weight fraction of chitin is 0.5-1 weight %.
Preferably, described bacillus cereus is bacillus subtilis, Bafillus natt, Bacillus licheniformis or wax bacillus cereus.
The present invention also provides for the preparation method of a kind of microbial pesticide, it is characterized in that, the method includes: a, by Vermiculitum, kieselguhr, Pulvis Talci, boehmite, Polyethylene Glycol, carbamide and water with weight ratio 1:(3-10): (15-35): (0.1-0.6): (0.1-0.6): (1-5): filter and be dried after (60-120) mix homogeneously, obtain the first solid；Wherein, described Vermiculitum, kieselguhr, Pulvis Talci, boehmite are with dry basis；B, the first solid of gained in step a is carried out roasting 1-10 hour at 350-430 DEG C, obtain the second solid；C, the second solid of gained in step c is pulverized after cross 20-100 mesh sieve, obtain the first powder；D, the first powder of gained in step c is carried out sterilizing after mix homogeneously with bacillus cereus mycelia, sodium alginate, sodium carboxymethyl cellulose and chitin, obtain microbial pesticide；Wherein, in described microbial pesticide, the weight fraction of sodium alginate is 0.1-3 weight %, and the weight fraction of sodium carboxymethyl cellulose is 0.1-3 weight %, and the weight fraction of chitin is 0.1-3 weight %, and the inoculation bacterium colony of bacillus cereus is 109-1010cfu/g.
Preferably, the weight ratio of described Vermiculitum, kieselguhr, Pulvis Talci, boehmite, Polyethylene Glycol, carbamide and water is 1:(5-7): (25-30): (0.2-0.4): (0.2-0.4): (2-3): (80-100), Vermiculitum described in step a, kieselguhr, Pulvis Talci, boehmite, Polyethylene Glycol, carbamide and water mix homogeneously carry out described filtration again and are dried after carrying out ultrasonic 1-10 minute；Wherein, described ultrasonic frequency is 50-120KHZ, and dry temperature is 100-200 DEG C, and the time is 1-3 hour.
Preferably, the temperature of roasting described in step b is 370-400 DEG C, and the time is 2-4 hour.
Preferably, described in step c, the mesh number of sieve is 50-80 mesh.
Preferably, carry out described filtration after being mixed together uniformly with Vermiculitum, kieselguhr, Pulvis Talci, boehmite, Polyethylene Glycol, carbamide and water by the hydrochloric acid that weight fraction is 1-5% and be dried；Wherein, hydrochloric acid is (0.1-1) with the weight ratio of Vermiculitum: 1.
Preferably, in step d, the preparation method of bacillus cereus mycelia includes: be inoculated in culture medium by the quantity of the inoculation bacterium colony of 0.3-1.5% by bacillus cereus, cultivating 1-10 days at 20-30 DEG C, take out culture and filter, the solid obtained is as bacillus cereus mycelia.
Preferably, bacillus cereus described in step d is bacillus subtilis, Bafillus natt, Bacillus licheniformis or wax bacillus cereus；In described microbial pesticide, the weight fraction of sodium alginate is 0.5-1 weight %, and the weight fraction of sodium carboxymethyl cellulose is 0.5-1 weight %, and the weight fraction of chitin is 0.5-1 weight %.
The invention have the benefit that
One, the bacillus cereus in pesticide of the present invention, at rhizosphere amount reproduction, defines dominant population at root system of plant, it is suppressed that the growth and breeding of pathogenic microorganism, reduces the generation of plant pest；Plant can be induced to produce the defense reaction of the involved in plant such as peroxidase of Antibiotics material and plant, improve the resistance against diseases of plant, the many kinds of substance such as siderophore and antibiotic, inducible system resistance can also be produced, produce the effect indirectly reaching to promote plant growing.
They are two years old, the microbial pesticide of the present invention contains multiple strong aminoacid, soluble chelating thing can be formed from word with metal, salinization of soil degree can be reduced, be conducive to farmland pollution thing degraded, microorganism produce polymer have drought-resistant, reduce water stress, improve Soil structure, supply organic nutrition of plant and regulation ion activity ability.
Its three, microbial pesticide inorganic elements can be converted into useful plants growth organic compound, improve redox of soil condition, be reduced to nitrogen deoxidation and oxidizing process, thus reduce nitrate content.The raw bacillus cereus of the rhizosphere growth-promoting of plant can promote the mechanism of plant growing, produces auxin, suppression pathogen, rhizosphere fixed nitrogen and decomposition insoluble phosphorus potassium element etc..
Its four, the microbial pesticide release rate of the present invention is high, containing multiple nutrients material, and the long-term preservation of beneficially pesticide.
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearer, technical scheme will be described in detail below.Obviously, described embodiment is only a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, other embodiments all that those of ordinary skill in the art are obtained on the premise of not making creative work, broadly fall into the scope that the present invention is protected.
The present invention provides a kind of microbial pesticide, it is characterized in that, this microbial pesticide includes Vermiculitum, kieselguhr, Pulvis Talci, aluminium oxide, bacillus cereus mycelia, sodium alginate, sodium carboxymethyl cellulose and chitin, wherein, described Vermiculitum, kieselguhr, the butt weight ratio of Pulvis Talci and aluminium oxide is 1:(3-10): (15-35): (0.1-0.6): (0.1-0.6), in described microbial pesticide, the weight fraction of sodium alginate is 0.1-3 weight %, the weight fraction of sodium carboxymethyl cellulose is 0.1-3 weight %, the weight fraction of chitin is 0.1-3 weight %, the inoculation bacterium colony of bacillus cereus is 109-1010cfu/g。
According to the present invention, Vermiculitum is a kind of natural, nontoxic mineral, dilatable mineral under high temperature action.It is the mineral that a kind of comparison is rare, belongs to silicate.Its crystal structure is monoclinic system, and from profile, it appears as Muscovitum.Vermiculitum is to produce during certain granite hydration.It typically produces with asbestos simultaneously.Owing to Vermiculitum has the ability that ion exchanges, it has effect greatly to the nutrition of soil.The butt weight ratio of described Vermiculitum, kieselguhr, Pulvis Talci and aluminium oxide can be 1:(5-7): (25-30): (0.2-0.4): (0.2-0.4), in described microbial pesticide, the weight fraction of sodium alginate can be 0.5-1 weight %, the weight fraction of sodium carboxymethyl cellulose can be 0.5-1 weight %, and the weight fraction of chitin is 0.5-1 weight %.
According to the present invention, bacillus cereus (Bacillus), a section of antibacterial, bacillus or the coccus of spore (endospore) can be formed.Including bacillus, Sporolactobacillus, fusobacterium, Desulfotomaculum and Sporosarcina etc..They to external world injurious factor resistance strong, distribution is wide, is present in soil, water, air and animal intestinal etc..Described bacillus cereus can be bacillus subtilis, Bafillus natt, Bacillus licheniformis or wax bacillus cereus.
The present invention also provides for the preparation method of a kind of microbial pesticide, it is characterized in that, the method includes: a, by Vermiculitum, kieselguhr, Pulvis Talci, boehmite, Polyethylene Glycol, carbamide and water with weight ratio 1:(3-10): (15-35): (0.1-0.6): (0.1-0.6): (1-5): filter and be dried after (60-120) mix homogeneously, obtain the first solid；Wherein, described Vermiculitum, kieselguhr, Pulvis Talci, boehmite are with dry basis；B, the first solid of gained in step a is carried out roasting 1-10 hour at 350-430 DEG C, obtain the second solid；C, the second solid of gained in step c is pulverized after cross 20-100 mesh sieve, obtain the first powder；D, the first powder of gained in step c is carried out sterilizing after mix homogeneously with bacillus cereus mycelia, sodium alginate, sodium carboxymethyl cellulose and chitin, obtain microbial pesticide；Wherein, in described microbial pesticide, the weight fraction of sodium alginate is 0.1-3 weight %, and the weight fraction of sodium carboxymethyl cellulose is 0.1-3 weight %, and the weight fraction of chitin is 0.1-3 weight %, and the inoculation bacterium colony of bacillus cereus is 109-1010cfu/g。
According to the present invention, kieselguhr is a kind of siliceous rock, is mainly distributed on the states such as China, the U.S., Denmark, France, Romania.Being a kind of biogenic silicastone, it is mainly made up of the remains of diatom in ancient times.Its chemical composition is with SiO2It is main, available SiO2·nH2O represents, mineralogical composition is opal and mutation thereof.The weight ratio of described Vermiculitum, kieselguhr, Pulvis Talci, boehmite, Polyethylene Glycol, carbamide and water can be 1:(5-7): (25-30): (0.2-0.4): (0.2-0.4): (2-3): (80-100), Vermiculitum described in step a, kieselguhr, Pulvis Talci, boehmite, Polyethylene Glycol, carbamide and water mix homogeneously carry out described filtration again and are dried after carrying out ultrasonic 1-10 minute；Wherein, described ultrasonic frequency can be 50-120KHZ, and dry temperature can be 100-200 DEG C, and the time can be 1-3 hour.
According to the present invention, the temperature of roasting described in step b can be 370-400 DEG C, and the time can be 2-4 hour.
According to the present invention, described in step c, the mesh number of sieve can be 50-80 mesh.
According to the present invention it is possible to carry out described filtration after being mixed together uniformly with Vermiculitum, kieselguhr, Pulvis Talci, boehmite, Polyethylene Glycol, carbamide and water by the hydrochloric acid that weight fraction is 1-5% and be dried；Wherein, hydrochloric acid is (0.1-1) with the weight ratio of Vermiculitum: 1.
According to the present invention, in step d, the preparation method of bacillus cereus mycelia may include that and is inoculated in culture medium by the quantity of the inoculation bacterium colony of 0.3-1.5% by bacillus cereus, cultivating 1-10 days at 20-30 DEG C, take out culture and filter, the solid obtained is as bacillus cereus mycelia.
According to the present invention, bacillus cereus described in step d can be bacillus subtilis, Bafillus natt, Bacillus licheniformis or wax bacillus cereus；In described microbial pesticide, the weight fraction of sodium alginate can be 0.5-1 weight %, and the weight fraction of sodium carboxymethyl cellulose can be 0.5-1 weight %, and the weight fraction of chitin can be 0.5-1 weight %.
The present invention will be further illustrated by embodiment below, but the present invention is not therefore subject to any restriction.
If no special instructions, instrument of the present invention and reagent are and are purchased, and the commodity of different brands do not affect specifically used.
Embodiment 1
Being inoculated in culture medium by the quantity of the inoculation bacterium colony of 1.5% by bacillus subtilis, cultivate 5 days at 20 DEG C, take out culture and filter, the solid obtained is as bacillus subtilis.
By Vermiculitum, kieselguhr, Pulvis Talci, boehmite, Polyethylene Glycol, carbamide and water to filter after weight ratio 1:3:15:0.1:0.1:1:60 mix homogeneously and to be dried, obtain the first solid；Wherein, described Vermiculitum, kieselguhr, Pulvis Talci, boehmite are with dry basis；First solid is carried out at 430 DEG C roasting 10 hours, obtains the second solid；Cross 100 mesh sieves after being pulverized by gained the second solid, obtain the first powder；Mix homogeneously with bacillus cereus mycelia, sodium alginate, sodium carboxymethyl cellulose and chitin after gained the first powder is carried out sterilizing, obtain microbial pesticide；Wherein, in described microbial pesticide, the weight fraction of sodium alginate is 3 weight %, and the weight fraction of sodium carboxymethyl cellulose is 3 weight %, and the weight fraction of chitin is 3 weight %, and the inoculation bacterium colony of bacillus cereus is 9.5 × 109cfu/g。
Embodiment 2
Being inoculated in culture medium by the quantity of the inoculation bacterium colony of 0.3% by Bacillus licheniformis, cultivate 10 days at 25 DEG C, take out culture and filter, the solid obtained is as Bacillus licheniformis silk.
By Vermiculitum, kieselguhr, Pulvis Talci, boehmite, Polyethylene Glycol, carbamide and water to filter after weight ratio 1:6:20:0.3:0.3:3:80 mix homogeneously and to be dried, obtain the first solid；Wherein, described Vermiculitum, kieselguhr, Pulvis Talci, boehmite are with dry basis；First solid is carried out at 350 DEG C roasting 5 hours, obtains the second solid；Cross 20 mesh sieves after being pulverized by gained the second solid, obtain the first powder；Mix homogeneously with bacillus cereus mycelia, sodium alginate, sodium carboxymethyl cellulose and chitin after gained the first powder is carried out sterilizing, obtain microbial pesticide；Wherein, in described microbial pesticide, the weight fraction of sodium alginate is 0.1 weight %, and the weight fraction of sodium carboxymethyl cellulose is 0.1 weight %, and the weight fraction of chitin is 0.1 weight %, and the inoculation bacterium colony of bacillus cereus is 8.7 × 109cfu/g。
Embodiment 3
Being inoculated in culture medium by the quantity of the inoculation bacterium colony of 1% by Bafillus natt, cultivate 1 day at 30 DEG C, take out culture and filter, the solid obtained is as Bafillus natt mycelia.
By Vermiculitum, kieselguhr, Pulvis Talci, boehmite, Polyethylene Glycol, carbamide and water to filter after weight ratio 1:10:35:0.6:0.6:5:120 mix homogeneously and to be dried, obtain the first solid；Wherein, described Vermiculitum, kieselguhr, Pulvis Talci, boehmite are with dry basis；First solid is carried out at 400 DEG C roasting 1 hour, obtains the second solid；Cross 80 mesh sieves after being pulverized by gained the second solid, obtain the first powder；Mix homogeneously with bacillus cereus mycelia, sodium alginate, sodium carboxymethyl cellulose and chitin after gained the first powder is carried out sterilizing, obtain microbial pesticide；Wherein, in described microbial pesticide, the weight fraction of sodium alginate is 1 weight %, and the weight fraction of sodium carboxymethyl cellulose is 1 weight %, and the weight fraction of chitin is 1 weight %, and the inoculation bacterium colony of bacillus cereus is 4.5 × 109cfu/g。
The release rate of the microbial pesticide that Application Example 1-3 is prepared for the inventive method is described.
The microbial pesticide prepared being placed in room temperature for storage, claims sample to join in the sterilized water with bead after 2d, by gradient dilution, coating, in detection preparation, the viable bacteria body quantity of release, is repeated 3 times.Release rate (%)=release bacillus cereus number (cfu)/inoculation bacillus cereus number (cfu) × 100%.
Table 1
It can be seen that the microbial pesticide release rate prepared of embodiment of the present invention 1-3 is high from the data of table 1.
The bactericidal property of the microbial pesticide that Application Example 4-6 is prepared for the inventive method is described.
Under greenhouse experiment, full cucumber seeds is sowed in the flowerpot filling with soil in advance, 3 strains in each basin, place it in greenhouse, when growing to tri-leaf period Deng plant, test liquid is sprayed on its blade, after 24h, inoculates cucumber powdery mildew's pathogen handle, bacterium face is affixed on cucumber leaves central authorities, and during cultivation, every day sprays proper amount of clear water to keep moistening.Calculate sickness rate after 10d, often process 3 times and repeat.Using clear water as comparison.Correlation computations formula: sickness rate=(morbidity strain number/investigate total strain number) × 100%.
Table 2
| Embodiment | Sickness rate, % |
| Clear water | 97.1 |
| Embodiment 1 | 18.9 |
| Embodiment 2 | 13.2 |
| Embodiment 3 | 15.3 |
From table 2 it can be seen that the microbial pesticide prevention effect of the present invention is good.
Claims (10)
1. a microbial pesticide, it is characterised in that this microbial pesticide includes Vermiculitum, kieselguhr, Talcum
Powder, aluminium oxide, bacillus cereus mycelia, sodium alginate, sodium carboxymethyl cellulose and chitin, wherein, described
The butt weight ratio of Vermiculitum, kieselguhr, Pulvis Talci and aluminium oxide is 1:(3-10): (15-35): (0.1-0.6):
(0.1-0.6), in described microbial pesticide, the weight fraction of sodium alginate is 0.1-3 weight %, carboxymethyl cellulose
The weight fraction of element sodium is 0.1-3 weight %, and the weight fraction of chitin is 0.1-3 weight %, the inoculation of bacillus cereus
Bacterium colony is 109-1010cfu/g。
Microbial pesticide the most according to claim 1, it is characterised in that described Vermiculitum, kieselguhr,
The butt weight ratio of Pulvis Talci and aluminium oxide is 1:(5-7): (25-30): (0.2-0.4): (0.2-0.4),
In described microbial pesticide, the weight fraction of sodium alginate is 0.5-1 weight %, and the weight of sodium carboxymethyl cellulose is divided
Number is 0.5-1 weight %, and the weight fraction of chitin is 0.5-1 weight %.
Microbial pesticide the most according to claim 1, it is characterised in that described bacillus cereus is hay
Bacillus cereus, Bafillus natt, Bacillus licheniformis or wax bacillus cereus.
4. the preparation method of a microbial pesticide, it is characterised in that the method includes:
A, by Vermiculitum, kieselguhr, Pulvis Talci, boehmite, Polyethylene Glycol, carbamide and water with weight ratio
1:(3-10): (15-35): (0.1-0.6): (0.1-0.6): (1-5): mistake after (60-120) mix homogeneously
Filter and be dried, obtain the first solid；Wherein, described Vermiculitum, kieselguhr, Pulvis Talci, boehmite are with dry
Basic weight gauge；
B, the first solid of gained in step a is carried out roasting 1-10 hour at 350-430 DEG C, obtain second
Solid；
C, the second solid of gained in step c is pulverized after cross 20-100 mesh sieve, obtain the first powder；
D, the first powder of gained in step c is carried out after sterilizing with bacillus cereus mycelia, sodium alginate, carboxylic first
Base sodium cellulosate and chitin mix homogeneously, obtain microbial pesticide；Wherein, Sargassum in described microbial pesticide
The weight fraction of acid sodium is 0.1-3 weight %, and the weight fraction of sodium carboxymethyl cellulose is 0.1-3 weight %, chitin
Weight fraction be 0.1-3 weight %, the inoculation bacterium colony of bacillus cereus is 109-1010cfu/g。
Preparation method the most according to claim 4, it is characterised in that described Vermiculitum, kieselguhr, Pulvis Talci,
The weight ratio of boehmite, Polyethylene Glycol, carbamide and water is 1:(5-7): (25-30): (0.2-0.4):
(0.2-0.4): (2-3): (80-100), the thin water of Vermiculitum described in step a, kieselguhr, Pulvis Talci, plan
Aluminum stone, Polyethylene Glycol, carbamide and water mix homogeneously carry out described filtration again and do after carrying out ultrasonic 1-10 minute
Dry；Wherein, described ultrasonic frequency is 50-120KHZ, and dry temperature is 100-200 DEG C, and the time is 1-3
Hour.
Preparation method the most according to claim 4, it is characterised in that the temperature of roasting described in step b is
370-400 DEG C, the time is 2-4 hour.
Preparation method the most according to claim 4, it is characterised in that described in step c, the mesh number of sieve is 50-80
Mesh.
Preparation method the most according to claim 4, it is characterised in that by hydrochloric acid that weight fraction is 1-5% with
Vermiculitum, kieselguhr, Pulvis Talci, boehmite, Polyethylene Glycol, carbamide and water are carried out after being mixed together uniformly
Described filtration and be dried；Wherein, hydrochloric acid is (0.1-1) with the weight ratio of Vermiculitum: 1.
Preparation method the most according to claim 4, it is characterised in that the system of bacillus cereus mycelia in step d
Preparation Method includes: be inoculated in culture medium by the quantity of the inoculation bacterium colony of 0.3-1.5% by bacillus cereus,
Cultivating 1-10 days at 20-30 DEG C, take out culture and filter, the solid obtained is as bacillus cereus mycelia.
Preparation method the most according to claim 4, it is characterised in that bacillus cereus described in step d is
Bacillus subtilis, Bafillus natt, Bacillus licheniformis or wax bacillus cereus；Described microbial pesticide
The weight fraction of middle sodium alginate is 0.5-1 weight %, and the weight fraction of sodium carboxymethyl cellulose is 0.5-1 weight %,
The weight fraction of chitin is 0.5-1 weight %.
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Application publication date: 20160727 |