KR20170014211A - Sustained release carrier having a Biological Control Activity of Plant disease, Biological Agent and The Method thereof - Google Patents
Sustained release carrier having a Biological Control Activity of Plant disease, Biological Agent and The Method thereof Download PDFInfo
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- KR20170014211A KR20170014211A KR1020150107173A KR20150107173A KR20170014211A KR 20170014211 A KR20170014211 A KR 20170014211A KR 1020150107173 A KR1020150107173 A KR 1020150107173A KR 20150107173 A KR20150107173 A KR 20150107173A KR 20170014211 A KR20170014211 A KR 20170014211A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- Pest Control & Pesticides (AREA)
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Abstract
Description
The present invention relates to a sustained-release biologic agent for controlling plant diseases, which has an excellent controlling effect on a plant disease, and more particularly to a sustained-release biologic agent for controlling a plant disease by obtaining a microorganism fermentation broth having an acidity controlled, impregnating the microorganism fermentation broth with a sustained- And to a sustained-release biologic preparation for controlling.
In Korea, 96.5% of synthetic pesticides, 0.5% of biological pesticides, and 3.0% of environment friendly agricultural materials are used as crop protection agents in Korea. However, synthetic pesticides among these crop protection agents are very toxic The manufacturer or user takes great care in handling it, and despite these precautions, there is a risk of lethal damage to the user.
Moreover, not only does it harm the consumer consuming agricultural chemicals contaminated with pesticides, or livestock being raised, but also poses a serious threat to the public health and destroys the ecosystem due to contamination of water quality and soil caused by the spraying of chemical pesticides .
Meanwhile, as international trade agreements such as the FTA are expected to come into effect one after another, in order to secure competitiveness for foreign agricultural products and increase agricultural exports to the US, Europe, and neighboring countries in Asia, The development and dissemination of pest control technology is urgent, and the government is attempting to convert it into an eco-friendly agricultural policy to reduce the use of pesticides and chemical fertilizers.
However, in Korea, the development of natural raw materials and eco-friendly substitutes to replace chemical pesticides and chemical fertilizers has been delayed, and the use of pesticides has not been reduced so much. Therefore, the development of environmentally friendly pesticides as fertilizers come.
As a means of solving these problems, most of the pesticides developed and marketed in Korea have been developed as microbial sustained bioproducts or metabolic products such as BT protein or plant extracts. And it is in the state of being imported as a finished product since imitation product is manufactured by importing main raw material or seed bacterium in a state where the patent right of the relevant strain is not clear.
Prior art disclosed in the prior art to provide biocidal pesticides has been disclosed in Korean Patent No. 1453447, which discloses a biocidal pesticide auxiliary composition comprising galectin as an active ingredient, a biocide composition containing galectin and bacillus thuringiensis, A polynucleotide encoding a galectin comprising a nucleotide sequence optimized to be expressed in Lymeenasis, an expression vector comprising the polynucleotide, a transformant transformed to express galectin by introducing the expression vector, A biocidal pesticide composition comprising a transformant is disclosed.
In addition, in Patent Publication 2015 27484, a BT-based biocidal pesticide composition capable of simultaneously controlling Chinese cabbage moths and moths moths is effectively mixed with Bithi producing Cry1 toxin protein and metabolites derived from Xn to produce Chinese cabbage moths and Chinese cabbage moths At the same time, a high-efficiency control agent capable of being killed within a short period of time has been proposed, but it has not been commercialized yet.
The present invention was initiated with a task to provide a sustained-release biological agent for controlling plant diseases, which is excellent in bactericidal effect against causative bacteria of various fungi and bacterial plant diseases.
The present invention provides a sustained-release sustained-release sustained-release microbial fermentation broth obtained by controlling the acidity of the microbial fermentation broth to improve the effectiveness of the control effect at agricultural sites, to prevent damage to crops, And to provide a sustained-release biological agent for controlling plant diseases.
In addition, the present application discloses that when a fermentation broth of a liquid microorganism is directly sprayed on lawn or cultivated plants, most of the active ingredients act on the plant or soil temporarily, halving the effect thereof, The present invention provides a sustained-release biologic preparation for controlling plant diseases, wherein the component of the carrier itself functions as a fertilizer component for supplying a trace element, and the microorganism fermentation liquid has a function of supplying a trace element to the carrier, And has the purpose of providing.
The present invention relates to a liquid fertilizer which is sprayed directly onto lawn or cultivated plants and, when used, most of the phytochemicals act on the plant or soil temporarily, resulting in only about 1/2 to 1/3 of the effect, 2/3 of the total amount of the microorganism fermentation broth, the microbial fermentation broth had a problem that the active ingredient of the microbial fermentation broth was damaged, and a function of inducing the microbial fermentation broth to release slowly while maintaining the effect of the microbial fermentation broth for a long period of time was required. Wherein the microorganism in the microbial fermentation broth functions as a fertilizer component and the non-hygroscopic component of the microbial fermentation broth cooperate with the plant to be cultivated.
The diatomaceous earth used in the present invention is a diatomaceous earth which is used as a carrier to be used for maintaining the fertilizer effect for a certain period of time and to increase the period of use. The diatomaceous earth used here is sedimentary rock deposited in the bottom layer after the diatoms living in lakes and marine sediments died.
The diatomaceous earth used by the present inventors was 12.5% in 20 mesh or more and 98.8% in 60 mesh or more through sieving, and had a surface area of 15 to 19 m 2 / g As a standard, diatomaceous earth having an oil absorption amount of 120% and a water absorption amount of 130% was used in a wide surface area.
The diatomaceous earth of the above composition is analyzed as a main component of silicon dioxide, but it is a photosynthetic material deposited on the bottom layer after the death of diatomic algae, which are naturally living in lakes or marine sediments, as iron, calcium, magnesium and Diatomaceous earth itself is not toxic to aquatic or terrestrial plants but has the effect as a natural insecticide in itself and has a high specific surface area and has the property of improving air permeability through pores and increasing water retention The inventors of the present invention can utilize the biocidal agent as a paste-like granule having a fertilizer and sterilizing function by impregnating the diatomaceous earth with the fermentation broth by using the characteristics of the diatomite.
Accordingly, the present inventor has proposed a method for obtaining a sustained-release biologic agent for controlling plant diseases, which is excellent in bactericidal effect against causative microorganisms of various fungi and bacterial plant diseases, which comprises culturing a microorganism culture solution containing water- The first step is to obtain a microbial fermentation broth having a pH of 3.4 ± 0.1 by fermentation in a thermostatic chamber maintained at 35 to 38 ° C in the range of 2 to 4 days by adding the microbial fermentation broth obtained from natural plants, And a second step of obtaining a sustained-release biological preparation for controlling a plant disease by impregnating 100 parts by weight of a sustained-release carrier material selected from rice bran, wheat bran and clay minerals at a ratio of 100 ± 50 parts by weight of the microbial fermentation broth obtained in the first step A sustained-release biological preparation for plant disease control can be obtained through a simple process comprising the steps of: The diatomaceous earth has a large amount of voids of up to 130% in water absorption. However, the rice bran clay minerals provide incompatible materials useful for the crops, but the water absorption amount is only 50 to 60%, and the use of diatomaceous earth is relatively .
The microbial fermentation broth used in obtaining the sustained-release biologic agent for plant disease control provided herein is obtained from a fruit or flower fruit which is provided naturally and the microbial fermentation broth is used in a pH range of 3.0 to 4.0. Since it exhibits alkalinity itself, it also has the effect of acting without stress on an organism that releases and slowly adsorbs an acidic microbial fermentation liquid to an alkali diatomaceous earth and releases it slowly.
The present invention relates to a sustained-release biologic agent for controlling plant diseases, which is used for preventing or treating plant diseases. The microorganism fermentation broth fermented to a pH of 3.4 ± 0.1 using a natural plant bacterium is dissolved in diatomaceous earth, rice bran, When a sustained-release biologic agent for controlling plant diseases of simple constitution provided by impregnating with a sustained-release carrier selected from clay minerals is applied to a plant in which a disease has occurred or may occur, Which is an environmentally friendly, sustained-release control agent exhibiting antimicrobial activity against bacteria and fungi which are the causative agents of bacteria and fungi.
The term "microbial fermentation broth" used in the present invention refers to healthy plants that do not contaminate natural ecosystems and have antibacterial activity against various plant diseases. Therefore, the term " It is preferable to use the seeds obtained. Then, a small amount of sugar is sprayed from fruit tree, lily fruit or magnolia fruit among the fruit trees, and then aged in a low temperature atmosphere of about 15 to 30 ° C for 20 to 40 days, The present inventors obtained the seeds through the above process, seeded the seeds with a small amount of molasses in a clean water, and seeded with seeds After about 2 to 4 days have elapsed, when the pH reaches the range of 3.4 ± 0.1, the microbial foot It could be used as a liquid.
In addition to the above diatomaceous earth components, carriers suitable for use in the sustained-release biologics for controlling plant diseases according to the present invention may be added with suitable solid carriers, liquid carriers or other suitable adjuvants as agriculturally acceptable excipients, For example, talc, clay, bentonite, pyrophyllite, kaolin, silica and the like may be used together as the solid carrier. The liquid carrier may further include a surfactant, an adhesive, and the like. The active ingredient of the sustained-release biologically active agent and the excipient may be mixed and used as an agricultural composition. The conventional method of preparing the composition for agricultural use may be used by using all conventional methods of application.
Therefore, in the present invention, a microorganism fermentation broth fermented so that the diatomaceous earth having a surface area in the range of 15 to 19 m 2 / g is used as a carrier and reached the range of pH 3.4 ± 0.1 by using the seed bacteria is impregnated into the carrier, The sustained-release biologic agent for plant disease control provided herein can be applied as a sustained-release biologic agent for plant disease control having a structure in which the fertilizer effect and the trace element supplying function of the carrier are applied so as to be gradually released to the plant to be cultivated. Various methods known in the art can be used to spray, apply, smear or the like to the plant or the environment, and the application concentration can also be determined by a person skilled in the art in accordance with the size, kind, growth condition, And the like.
The sustained-release biologic agent for plant disease control provided in the present invention is a non-toxic, non-toxic, non-toxic, non-toxic, non-toxic, non- It has an effect of providing a non-polluting disinfectant.
When the "microbial fermentation broth" used in the present invention is sowed on a lawn or cultivated plant as a liquid phase, the effect of controlling the plant disease of the microbial fermentation broth is reduced to about 1/2 to 1/3, The effect of controlling the plant disease is 80 ~ 99% and the effect is gradually eluted.
Fig. 1: Manufacturing process diagram for obtaining the sustained-release biologic agent of the present invention.
Hereinafter, the present invention will be described in detail with reference to the following examples. It should be understood, however, that the following examples are intended to illustrate but not limit the scope of the present invention.
1 is a diagram showing a production process for obtaining a sustained-release biologic agent to which the technical idea of the present application is applied. The sustained-release biologic agent for plant disease control provided herein is obtained by collecting seed bacterium from a natural plant and, for example, 25 kg of molasses and 5 kg of rice were weighed and then 2 kg of sugar was added to the solution to obtain a microorganism fermentation broth obtained by fermenting the fermentation broth for 2 to 4 days and slowly discharging the fermentation broth of the microorganism. In order to obtain a sustained-release biological preparation for controlling a plant disease by packaging a microorganism fermentation broth in a unit of 10 kg to 20 kg by impregnating with a fermentation broth of 100 ± 50, the following description will sequentially describe the production examples applicable to each step .
Production Example (1)
Since the present technology is aimed at living animals, it is preferable to use an organism obtained from a natural plant not artificially by changing the environment or processed by a chemical process or chemical process. Therefore, the fruit of the fruit tree, In a low temperature atmosphere of 15 to 30 ° C for 20 to 40 days, and when cotton or hemp is covered on top of it, the seeds are applied to the top of cotton or hemp so that the seeds are brought into a white fungus state, When you want to shorten the period, it is aged in a low temperature atmosphere of 15 ~ 30 ℃ for 10 days after subtotal fruit, lily fruit or magnolia fruit and after adding a little sugar or molasses slightly, about 3 ~ 4 days later, To obtain a microbial fermentation broth by using the above-mentioned seeds.
Example 2 (Preparation of microbial fermentation broth)
As a means for obtaining a microorganism fermentation broth as a raw material for obtaining the sustained-release biological agent for controlling plant diseases, the present invention provides a method for producing a fermentation broth of plant microorganism, which comprises obtaining 25 kg of molasses, 1.0 kg of the seed obtained in the above Preparation Example (1) And about 2 to 4 days are elapsed. If the pH is in the range of 3.2 to 3.5, it can be used as a microbial fermentation solution used in the present invention.
Example 3: Preparation of sustained-release bioproducts for controlling plant diseases [
Since the microbial fermentation broth obtained from Preparation Example (2) is provided as a liquid phase, when it is impregnated with the diatomaceous earth support shown in Table 1, the diatomite has a mesh size of 12.5% or more through 20% ) Was 98.8% and the surface area was 15 ~ 19 ㎡ / g. Using the diatomite, it was easily sprayed on the plant cultivation site, And to provide a sustained-release biologic preparation for control.
Field Test Application Example of Microbial Fermentation Liquid from Preparation Example (2) (4)
The inventors of the present invention found that the microbial fermentation broth obtained in Production Example (2) was subjected to 3 times as much water as a means for confirming whether the microbial fermentation broth sample product obtained in Production Example (2) had the expected antimicrobial effect and ineffective effect As a result of spraying with sprayer in the form of liquid fertilizer in the garden of the company, it was found that the effect of preventing lawn disease was better than that of organic liquid fertilizer sprayed in the past and that the grass grew fresh.
Field test application of a sustained-release biological agent for controlling plant diseases (5)
As a means for confirming the effect of controlling the golf course of the sustained-release biological agent for plant disease control obtained in the above Production Example (3), the grass area of the S country club golf course in Gwangjak-si, Gyeonggi-do, The sustained-release biocontrol agent for plant disease control obtained in the above-mentioned Production Example (3) was applied to each 4-m 2 for 30 days so as to spray 400 g / m 2 once every three days, To apply the vegetable grass control agent provided by K-Bio for 4 days to 400g / ㎡ for 3 days once a day for 3 days, the use of both sides was checked.
The growth conditions of the lawn were smooth and soft with the leaves of the grass in both products of both the sample product and the golf course lawn care biological product supplied from K-Bio, and it was dark green, good root growth and vigorous rooting rate The results were confirmed. However, in the test results of the aphid control test, it was confirmed that the one-half-sized pod of the present sample product was transferred to the grass where aphids died a day and other aphids were not sprayed with the sample product of the present invention However, in the golf course lawn care biologics imported and supplied from K-Bio, aphids did not disappear or diminished, and the results were more vigorous.
Therefore, the sustained-release biologic preparation for plant disease control provided in the preparation example (3) of the present invention can attain satisfactory results in both growth and development of turfgrass, there was.
Field test application of a sustained-release biological agent for controlling plant diseases (6)
The inventor of the present invention has found that when a sustained-release biologic preparation for controlling plant diseases obtained in the above-mentioned Production Example (3) is used as a means for confirming the ineffectiveness and control effect of strawberry, 1/3 of the cultivation area is obtained at the strawberry farm in Namyangju- The results are shown in Table 2. The results are shown in Table 2. [Table 2] < tb > < TABLE >
As can be seen from the above Table 2, the strawberry obtained in the area where the sustained-release bioprostheses for controlling plant diseases according to the present invention were used was 145% higher than that of the untreated area (grown without application) Roots and stem were also observed to grow firmly.
Therefore, the sustained-release biological agent for controlling plant diseases to which the technical idea of the present application is applied is a natural product that minimizes the use of chemical pesticides conventionally used for greenhouse or special crop cultivation such as a greenhouse or a green house, It is anticipated that it will be possible to provide a sustained-release biocide exhibiting non-toxic, non-pollution-free sterilization characteristics that do not pollute the ecosystem.
FIG. 1 shows a manufacturing process diagram for obtaining a sustained-release biologic preparation of the present invention. Since no separate apparatus is used, a separate reference numeral is not necessary.
Claims (5)
A first step of obtaining a microorganism culture solution containing a component selected from molasses, rice, and sugar in a purified water to produce a microorganism fermentation broth having a pH of 3.4 ± 0.1 by fermenting the microorganism in a range of 2 to 4 days;
100 parts by weight of a carrier material selected from diatomaceous earth, rice bran, wheat bran and clay minerals as a microbial agent carrier is impregnated with the microbial fermentation broth obtained in the first step at a ratio of 100 + - 50 parts by weight to obtain a sustained- step;
Wherein the method comprises the steps of:
Wherein the microorganism fermentation broth used in the first step is obtained by using a seed obtained from fruit or flower fruit and is adapted to be used in a pH range of 3.3 to 3.5.
Wherein the diatomaceous earth is chemically inert and has a surface area in the range of 15 to 19 m < 2 > / g in the application of the second step.
The microbial fermentation broth was fermented so that the diatomaceous earth having a surface area of 15 ~ 19 ㎡ / g was used as a carrier and reached the range of pH 3.4 ± 0.1 by using the bacterium. Wherein the composition is applied so that the trace element supplying function of the carrier is gradually released to the plant to be cultivated.
Wherein the carrier used in the sustained-release biocide is selected from talc, clay, bentonite, pyrophyllite, kaolin or silica instead of diatomaceous earth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020150107173A KR20170014211A (en) | 2015-07-29 | 2015-07-29 | Sustained release carrier having a Biological Control Activity of Plant disease, Biological Agent and The Method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| KR1020150107173A KR20170014211A (en) | 2015-07-29 | 2015-07-29 | Sustained release carrier having a Biological Control Activity of Plant disease, Biological Agent and The Method thereof |
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| KR20170014211A true KR20170014211A (en) | 2017-02-08 |
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| KR20200056361A (en) | 2020-05-04 | 2020-05-22 | 김용원 | The Generator with Footboard |
| KR20200060307A (en) | 2020-05-06 | 2020-05-29 | 김용원 | The Generator with Bicycle and Rear Carrie (2connected Turbine) |
| KR20200060309A (en) | 2020-05-08 | 2020-05-29 | 김용원 | The Generator with Health Vehicle |
| KR20200060308A (en) | 2020-05-07 | 2020-05-29 | 김용원 | The Generator with Aqua board |
| KR20200061324A (en) | 2020-05-11 | 2020-06-02 | 김용원 | The Generator with a Boat |
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| KR20200060307A (en) | 2020-05-06 | 2020-05-29 | 김용원 | The Generator with Bicycle and Rear Carrie (2connected Turbine) |
| KR20200060308A (en) | 2020-05-07 | 2020-05-29 | 김용원 | The Generator with Aqua board |
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| KR20200061324A (en) | 2020-05-11 | 2020-06-02 | 김용원 | The Generator with a Boat |
| KR20200062092A (en) | 2020-05-11 | 2020-06-03 | 김용원 | Air Turbine type Generator connected Wheels type Generator used by Falling water & Air Compressor |
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| KR20200083393A (en) | 2020-06-16 | 2020-07-08 | 김용원 | Drone with generators that change the rotation direction of the propeller motor using the Peltier element as a power generation element |
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| KR20210040852A (en) | 2021-03-24 | 2021-04-14 | 김용원 | The airbus electric(Generator) |
| KR20210043513A (en) | 2021-03-26 | 2021-04-21 | 김용원 | A device that collects fire smoke, volcanic fumes and ash installed in airbus electric(Generator) |
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| KR20210049046A (en) | 2021-04-13 | 2021-05-04 | 김용원 | Auxiliary wings and auxiliary door devices to rescue airbus electric(Generator) from strong winds and typhoons |
| KR20210055643A (en) | 2021-04-27 | 2021-05-17 | 김용원 | An electric board with a large number of rotor wheels and a large number of stator wheel generators on a motorized shaft |
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| KR20210080293A (en) | 2021-06-11 | 2021-06-30 | 김용원 | Generator case |
| KR20220016234A (en) | 2022-01-14 | 2022-02-08 | 김용원 | A 3wheeled bicycle equipped with an air thruster that combines an air pump and an air turbine |
| KR20220038026A (en) | 2022-02-24 | 2022-03-25 | 김용원 | 4 connected fans on an axle turbine : 1. The inner fans on the axle in inner cylinder, 2. The outer fans on the axle in outer cylinder &3. The duct fans on the outer fans inside in 2 outer cylinders and 2 inner cylinders, 4. The blades on the duct fans ring in the duct with an inlet and an oulet with another Cylinder |
| KR20220056159A (en) | 2022-04-04 | 2022-05-04 | 김용원 | A generator connected to a steam cleaner (powered by a steam turbine with a super heater) and Air filter (air intake, cold and hot air exhaust) A system that combines an air purifying facility and an ice maker in conjunction with an air turbine |
| KR20220056835A (en) | 2022-04-18 | 2022-05-06 | 김용원 | An aqua bicycle equipped with 3 air thrusters that combines an air pump and an air turbines |
| KR20220076421A (en) | 2022-05-11 | 2022-06-08 | 김용원 | A pedal vehicle |
| KR20220080053A (en) | 2022-05-27 | 2022-06-14 | 김용원 | An air layers tube boat |
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