KR20150116081A - Seed coating agent containing perlite for enhancing effect of Leguminous Manures growht in the high salinity land - Google Patents

Abstract

The present invention relates to a seed coating agent of beans and green manure crops, a manufacturing method thereof, and a method for coating seeds of beans and green manure crops by using the seed coating agent. Specifically, a seed coating agent mixed with a Rhizobium sp. strain and perlite can be usefully used for a seed coating agent for promoting germination and growth of the seeds of beans and green manure crops by mixing the Rhizobium sp. strain and the perlite, a manufacturing method thereof, and a method for coating seeds of beans and green manure crops by using the seed coating agent, by confirming effects in promoting germination and increasing growth of the seed of beans and green manure crops in soil in a hypersaline environment.

Description

[0001] The present invention relates to a seed coating agent containing perlite and a green seed grain coating composition containing a perlite for enhancing the growth of leguminous manures in the high salinity land,

The present invention relates to a soybean and green grass seed coating agent, a method for producing the same, and a coating method of soybean and green grass seeds using the seed coating agent.

Nitrogen fixation refers to the process of converting stable inert nitrogen molecules present in large amounts in air into other highly reactive nitrogen compounds (ammonia, nitrate, nitrogen dioxide, etc.). Nitrogen-fixing bacteria are nitrogen-fixing bacteria. Nitrogen-fixing bacteria are typically found in soybean roots. Nitrogen-fixing bacteria undergo biological nitrogen fixation, and biological nitrogen fixation is sometimes referred to as nitrogen assimilation. Means the conversion of nitrogen or inorganic nitrogen compounds in the atmosphere into organic nitrogen compounds by the action of an organism.

The two crops are not only able to grow by fixing nitrogen in the air through symbiotic relationship with the rhizobia genus, which is a root bacterium, but also can reduce the nitrogen application capacity because other crops can use the nitrogen fixed by the two crops. . In order to grow a stable two-crop crop, it is essential to inoculate the root with excellent nitrogen fixation. As a typical bacterium among nitrogen-fixing bacteria, there are root-knot bacteria. The root bacterium is a gram-negative soil bacterium of Rhizobium sp., Also known as rhizobium. It is symbiotic (or parasitic) with two crops and fixes nitrogen in the air. Changes in the density of the root bacilli in the soil are related to the number of years of cultivation of the soybeans. Native root formation and nitrogen fixation can not be expected unless the soybean is cultivated for many years or the new breeding grounds are not cultivated at all.

Although Korea has a large population, more than 60% of the whole country is a mountain, so it is difficult to supply the necessary paper for new industrial parks, housing complexes and ports required for economic development. Accordingly, in order to supply the necessary paper, a project has been carried out to supply these papers by changing to the land, though it was originally a sea or a river. For the purpose of prevention of disaster of agriculture and fisheries industry and increase of productivity, the State and local autonomous entities are required to preserve and manage the tide bank management law. However, in the case of reclaimed land, the soil is filled with soil, but its effectiveness is inferior due to the high concentration of salt in the soil. Therefore, there is a need to increase the plant utility of reclaimed land not only in local governments but also in national level.

There are various solutions to solve the problem of accumulation of salts in the soil. Especially, improvement of soil condition and improvement of seaweed method to minimize damage and development of salt tolerant crops have been carried out to date. Most crops are neutral crops (glycophyte), which are sensitive to salt accumulation and exhibit growth retardation and yield reduction. The lethal effects of salt accumulation on plant growth are known to be due to the following factors. 1) The plant growth is greatly affected by the low osmotic pressure of the soil solution compared to the plant, 2) the nutrient imbalance, 3) the ion effect which harms the plant, and 4) the combined effect of the above factors. In the plant body, major metabolic processes such as photosynthesis, protein synthesis, carbohydrate and fat metabolism are influenced by the degree of salting, resulting in growth inhibition and reduction in yield.

In order to improve the fertility of soils such as reclaimed land, it is necessary to cultivate green foliage crops that can fix nitrogen, and they are exploring ways to help plant growth by using salt tolerant microorganisms. In order to improve the soil fertility of the reclaimed soil, it is necessary to use the same green color as the above crops. However, until now, hairy vetch was used as a green coloring and hairy vetch was inoculated to increase the nitrogen fixing rate There have been no studies on the inoculation of root cancers.

Seed pellets are used to bond the coating material to the seed surface after the adhesive material is applied to the seed surface. The coating materials are capable of bonding to some extent when water is added. However, in transportation or in farming, There is a lot of concern, and the bonding force between the seed and the covering material must be strengthened. Therefore, a supplementary adhesive material is required in addition to moisture. The adhesive material must be easy to form seeds without reducing seed germination at a certain concentration. Known seed electrodeposits include methyl cellulose, carboxymethyl cellulose carboxymethyl cellulose, gum, starch, polyvinyl alcohol, cyclo dextrin, and the like. It is reported that the effect of methyl cellulose and gum on the binding strength of adhesive and covering materials in seed pelleting is excellent, It has not been used as a seed electrodeposition agent.

Accordingly, the present invention relates to Korean Patent Laid-Open No. 10-2013-0042861 (a hairy rheumatoid arthritis resistant rubybacterium strain) to which the present inventors have rights. The strains were found to be excellent in growth in a high salt environment and had excellent nodule forming ability and nitrogen fixation ability when they were inoculated with hair ribbing. This strain has been deposited with the Genetic Bank of Korea (KCTC) under accession number KCTC 12024BP. The inventors of the present invention have found that, in the course of continuous research using the strain, the present inventors have found that when hairy vetch seeds are coated with a coating agent comprising a mixture of the strain and perlite, the germination rate of hairy vetch, A seed coating agent for promoting germination and growth of soybean and green crop seeds, a method for producing the same, and a method for producing the seed coating agent by mixing the Rhizobium sp. Strain and perlite, The present invention has been accomplished on the basis of the fact that it can be usefully used as a coating method of soybean and green grass seeds.

It is an object of the present invention to provide a coating agent for soybean and green grass seeds including hair ribbing using pearlite.

Another object of the present invention is to provide a method for producing the seed coating agent.

Another object of the present invention is to provide a method for promoting germination and promoting growth by coating seeds of soybean and green crops including hair ribbing using the seed coating agent.

In order to achieve the above object,

Carboxylmethyl cellulose (CMC) and Arabic gum (AG) as electrodeposition agents; And

There is provided a soybean seed and a green grass seed coat coating agent containing perlite and Rhizobium sp. As a coating agent.

In addition,

i) mixing a carboxylmethyl cellulose (CMC) at a concentration of 0.5 to 2% (v / v) and an Arabic gum (AG) at a concentration of 1-2% (v / v) step; And

( Ii ) a step of mixing the perlite and Rhizobium sp. Strains to prepare a coating agent, wherein the coating agent comprises an electrodeposition agent and a coating agent.

In addition,

i) treating the seeds of soybean and green crops with carboxylmethyl cellulose (CMC) and Arabic gum (AG) as an electrodeposition agent and coating them first; And

Ii ) treating the perlite and Rhizobium sp. Strains as a coating to effect secondary coating, thereby providing a method of promoting or promoting the germination rate of soybean and green crop seeds.

The invention leguminous green manure crop seed coating agent, relates to a production method thereof, and a coating method of leguminous green manure crop seeds with the seed coating agent, and specifically Li Jovi ium in (Rhizobium Seed coatings containing a mixture of a strain and a perlite were found to be effective in promoting germination and promoting growth of soybean and green crop seeds in high salt environment so that the Rhizobium sp. strain and perlite ) Can be used as a seed coating agent for promoting germination and promoting growth of soybean and green grass seeds, a method for producing the same, and a coating method for soybean and green grass seeds using the seed coating agent.

FIG. 1 is a diagram showing the treatment of hairy vetch seeds by mixing different strains of the strain RHH84 ( Rhizobium sp. RHH84) with different strains of hairy roots and selecting highly stable clones.
FIG. 2 is a view showing the stability of coating according to storage time and temperature by mixing Rhizobium sp. RHH84 strain with perlite.
FIG. 3 is a graph showing the effect of germination rate and growth promoting effect of hairy vetch seeds coated with hairy vetch seeds by mixing Rhizobium sp. RHH84 strain with perlite Also:
1) perlite single treatment group; And
2) Perlite + Rhizobium sp. RHH84 strain.

Hereinafter, the present invention will be described in detail.

The present invention relates to a composition comprising carboxylmethyl cellulose (CMC) and Arabic gum (AG) as an electrodeposition agent; And

There is provided a soybean seed and a green grass seed coat coating agent containing perlite and Rhizobium sp. As a coating agent.

The Rhizobium sp. Strain is preferably Rhizobium sp. RHH84 deposited with Accession No. KCTC12024BP.

The production of pellet seeds can produce densified pellet seeds by spraying the adhesive to continuously add the pellet coating material to the seed coat (Scott, 1989).

A binder, polymer, is injected onto the seed surface and begins by bonding the seed to the pellet material. Pellet coat materials can be agglomerated while rotating in a pellet container because of their ability to agglomerate to some extent, but an electrodeposite is needed to enhance the bondability, and pelletized seeds without the electroplating agent tend to break. The role of the electrodeposition is important in the binding between the seed and the coat material, but should not impair seed germination. The selection of the electrodeposition agent should be different according to the water solubility, the constant hardness to withstand the rupture, the substrate affinity and the degree of agglomeration. Generally, the electrodeposition agent such as carboxylmethyl cellulose (CMC), polyvinyl alcohol (polyvinyl alcohol) PCA), methyl cellulose, Tween 80, polyvinyl pyrrolidinone (PVP) or hydroxyethyl cellulose (HEC) may be used, and it is more preferable to use carboxyl methyl cellulose.

In addition, compared to other electrodeposits, it is easy to granulate, has excellent hardness and hardness, and is water-soluble. Therefore, when the granules absorb moisture, it is easily decomposed and used as a powder. Arabic gum (AG) . Therefore, the theory that can be used as an electrodeposition agent for seeds using carboxyl methyl cellulose and gum arabic was confirmed. In a specific embodiment of the present invention, the present inventors collected hairy vicia villosa Roth seeds, and selected healthy hairy vetch seeds, except for the light-spotted seeds, from the collected seeds to obtain carboxymethyl cellulose , And CMC) and arabic gum (AG) were selected as hairy vetch seed coated electrodeposits. The hair germination rates of 1.0, % And germination rate of the group treated with carboxymethylcellulose at the concentration of 0.5 and 1.0% were similar to those of the untreated group, and both of the whole plants were titrated to hairy vetch seeds It was confirmed that it can be used as a electrodeposition agent. The germination rate (55%) treated with arabic gum 1% and the germination rate (55%) treated with 1% carboxymethyl cellulose were superior to the germination rate (50%) of the untreated group (See Table 1). ≪ tb > < TABLE > At this time, as the electrodeposition agent of the hairy vetch seeds, carboxylmethyl cellulose (CMC) at a concentration of 0.5 to 2% (v / v) and arabic gum at a concentration of 1 to 2% (v / v) AG), and it is preferable to use a mixture of carboxymethyl cellulose (CMC) and arabic gum (AG) at a concentration of 1% (v / v) at a concentration of 0.5 and 1% (v / v) It is more preferable to mix and process them.

The coating material is used as an extender to increase the size of the pellet seed. To date, the coating materials used in seed pellets have been classified as clay materials such as montmorillonite and clay materials such as chalk, sawdust, sand, cellite, krilium, bentonite clay bentonite clay, calcium carbonate, diatomaceous earth, zeolite, talc, bauxite, peat moss and vermiculite are mainly used. (Scott, 1989; Taylor et al., 1990). The perlite, which is a clone of the hairy vetch seed, can not be evacuated to the atmosphere during the cooling of volatile gases, water, etc. due to the quenching of magma in the atmosphere as a result of the volcanic activity of the Cenozoic era, It can be regarded as secondary minerals that exist together with crystal water in the ball. The pearlite has been used as a lightweight soil material until now as a secondary mineral and has been conventionally used as a building material. The pearlite pulverized product is washed with water, dried at 150-200 ° C and then mixed with an electrodeposition agent. come. These pearlite expanded processing stones are generally lightweight and porous, and are listed as food additive (filtration) as approved by domestic and overseas organic agriculture in addition to building materials (CODEX, IFOAM). They are also used for rooftop planting and grass cultivation. The pearlitic stones are mixed with peatmoss, bark, zeolite and coco peat for plant control.

The Rhizobium sp. Strain is a nitrogen-fixing bacterium that fixes nitrogen and is a typical example of nitrogen-fixing bacteria in the roots of soybean roots. Nitrogen-fixing bacteria are among the most common germs. The root-borne bacteria are Rhizobium sp.) gram-negative soil bacteria, also known as rhizomes. It is symbiotic (or parasitic) with soybean and crops and fixes nitrogen in the air. Therefore, the theory that can be used as a seed coat using pearlite and lysozyme can be confirmed. In a specific embodiment of the present invention, the present inventors have found that hairy vicia villosa Roth has a nodule-forming ability and a nitrogen fixation ability, which are isolated from hairy vicia villosa Roth in order to select hairy vetch seeds with high- Rhizobium ( Rhizobium ) Rhizobium ( Rhizobium) sp. RHH84) (KCTC12024BP) were mixed with different types of pharmacopoeia to determine the number of viable cells in each preparation. As a result, Rhizobium RHH84 sp. RHH84) (KCTC12024BP) and pearlite were mixed with bentonite, kaolin, and pyrophyllite, the number of viable bacteria was 4 to 20 times higher than the viable count of bacteria treated with the mixture of bentonite, kaolin and pyrophyllite (see FIG. 1) . In addition, in order to confirm the stability of the preparation of the strain RHL84 ( Rhizobium sp. RHH84) (KCTC12024BP) containing perlite, the stability of the clotting was evaluated according to storage time and temperature. As a result, Perlite-blended hair-rhea nodules RHY84 ( Rhizobium sp. RHH84) (KCTC12024BP) increased from 3 weeks at different temperature conditions, and it was confirmed that stable viable cell count was maintained continuously for 4 to 6 months (see FIG. 2) Genus RHH84 ( Rhizobium sp. RHH84) (KCTC12024BP) and pearlite may be mixed to be useful as a coating agent for hair resting seeds.

 It is preferable to mix perlite and Rizobygous sp. Strain as a coating agent in a weight ratio of 1: 1.

Preferably, the Ribobacterium strain contains 2 × 10 ⁵ cfu / ml to 2 × 10 ¹⁰ cfu / ml, more preferably 2 × 10 ⁷ cfu / ml. desirable.

The myofiber root inoculum may contain the strain or a culture thereof as an active ingredient. The inoculum of the present invention can be formulated for promoting plant growth by a conventional method and can be prepared in the form of a dry powder or a liquid fertilizer. Specifically, the inoculum of the present invention can be granulated in the form of a liquid fertilizer, but the formulation is not particularly limited. Specifically, it can be formulated as a plant growth-promoting biologic fertilizer for replacing chemical fertilizer, that is, it can be formulated as a biological fertilizer to overcome it in environmentally friendly organic agriculture with limited supply of chemical fertilizer.

The inoculum of the present invention may be prepared by a method known to a person skilled in the art, and the application method thereof may be a conventional method, that is, spraying (for example, spraying, misting, atomizing, powder spraying, granule spraying, (For example, coating, smearing, coating or the like), immersion, poisoning, smoking, application, etc. can be carried out. The amount of use can be appropriately determined depending on the body type, the damage situation, the application method, and the application place.

The herbicidal inoculation agent may be used in a liquid state, in a plant-like manner, in a seed of a crop, or in a seed coat, but is not limited thereto.

In the case of the above immersing method, the strain, the culture liquid thereof or the herbicidal inoculum may be poured into the soil around the plant, or the seed may be immersed in the culture medium and the preparation. In the case of spraying, But it is not limited thereto.

Preferably, the soybean and green crops are any one selected from the group consisting of hairy bean curd, chrysanthemum, clover, and nema vermiculite, but the present invention is not limited thereto.

It is preferable that the seed coating agent has an effect of promoting seed germination and promoting growth in a soil of high salt environment.

The soil in the high salt environment is preferably one selected from the group consisting of a reclaimed land, a sea landfill, a vinyl house, and a salt accumulating soil, but is not limited thereto.

In the method, the perlite and rhizobium sp.) is preferably stored at a temperature of 5 ° C to 30 ° C, more preferably at a temperature of 4 ° C to 28 ° C.

In the above method, the perlite and Rhizobium sp. Strains are preferably stored for 5 to 10 months, more preferably 4 to 6 months.

In addition,

i) mixing a carboxylmethyl cellulose (CMC) at a concentration of 0.5 to 2% (v / v) and an Arabic gum (AG) at a concentration of 1-2% (v / v) step; And

( Ii ) a step of mixing the perlite and Rhizobium sp. Strains to prepare a coating agent, wherein the coating agent comprises an electrodeposition agent and a coating agent.

It is preferable to mix the perlite and the Rizobacterium strain of step i) in a weight ratio of 1: 1.

The concentration of carboxylmethyl cellulose (CMC) in step i) is preferably 0.5 to 2% (v / v), more preferably 0.5 to 1.0% (v / v).

The concentration of arabic gum in the step i) is preferably 1 to 2% (v / v), more preferably 1.0, 1.5 and 2.0% (v / v).

Preferably, the step (ii) includes 2 × 10 ⁵ cfu / ml to 2 × 10 ¹⁰ cfu / ml, more preferably 2 × 10 ⁷ cfu / ml.

The seed coating material obtained by mixing Rhizobium sp. (KCTC12024BP) strain and perlite according to the present invention is effective in promoting germination and promoting growth of soybean and green crop seeds in high salt environment soil, Rhizobium sp. (KCTC12024BP) strain and perlite can be used as a seed coating agent for promoting germination and growth of soybean and green crop seeds.

In addition,

i) treating the seeds of soybean and green crops with carboxylmethyl cellulose (CMC) and Arabic gum (AG) as an electrodeposition agent and coating them first; And

Ii ) treating the perlite and Rhizobium sp. Strains as a coating to effect secondary coating, thereby providing a method of promoting or promoting the germination rate of soybean and green crop seeds.

The concentration of arabic gum in the step i) is preferably 1 to 2% (v / v), more preferably 1.0, 1.5 and 2.0% (v / v).

Preferably, the step (ii) includes 2 × 10 ⁵ cfu / ml to 2 × 10 ¹⁰ cfu / ml, more preferably 2 × 10 ⁷ cfu / ml.

 Preferably, the soybean and green crops are any one selected from the group consisting of hairy bean curd, chrysanthemum, clover, and nema vermiculite, but the present invention is not limited thereto.

It is preferable that the above method has the effect of promoting germination and promoting growth of soybean and green grass seeds in a soil of high salt environment.

The myofiber root inoculum may contain the strain or a culture thereof as an active ingredient. The inoculum of the present invention can be formulated for promoting plant growth by a conventional method and can be prepared in the form of a dry powder or a liquid fertilizer. Specifically, the inoculum of the present invention can be granulated in the form of a liquid fertilizer, but the formulation is not particularly limited. Specifically, it can be formulated as a plant growth-promoting biologic fertilizer for replacing chemical fertilizer, that is, it can be formulated as a biological fertilizer to overcome it in environmentally friendly organic agriculture with limited supply of chemical fertilizer.

The inoculum of the present invention may be prepared by a method known to a person skilled in the art, and the application method thereof may be a conventional method, that is, spraying (for example, spraying, misting, atomizing, powder spraying, granule spraying, (For example, coating, smearing, coating or the like), immersion, poisoning, smoking, application, etc. can be carried out. The amount of use can be appropriately determined depending on the body type, the damage situation, the application method, and the application place.

The herbicidal inoculation agent may be used in a liquid state, in a plant-like manner, in a seed of a crop, or in a seed coat, but is not limited thereto.

In the case of the above immersing method, the strain, the culture liquid thereof or the herbicidal inoculum may be poured into the soil around the plant, or the seed may be immersed in the culture medium and the preparation. In the case of spraying, But it is not limited thereto.

In the present invention, in order to have the effect of promoting germination and enhancing the growth of hairy vetch seeds in the soil of high salt environment, the seed coat is first coated with an adhesive agent having excellent adhesive strength, and then, Coating method was used. Namely, carboxylmethyl cellulose (CMC) and Arabic gum (AG) were first coated as an electrodeposition agent of hairy vetch seeds and treated with perlite containing Rhizobium sp. perlite), it is desirable to stably maintain the adhesive force at the same time as the effective inoculation of the strain of the genus Rizobacterium.

In a specific embodiment of the present invention, the inventors of the present invention treated a hair-reeved seed with a coating preparation prepared by mixing a strain of hairybee's RHH84 ( Rhizobium sp. RHH84) (KCTC12024BP) and perlite, The germination rate and growth promoting ability of the seeds were examined. The germination rate of the control group was found to be 30%. The germination rate of the control group was 30%, and the coating of a mixture of Rhizobium sp. RHH84 (KCTC12024BP) strain and perlite In the group treated with the preparation, germination rate of about 70% or more was confirmed. As a result of confirming hairy vesicle growth on the ground part, it was confirmed that the growth of the group treated with the hair follicle of the present invention was effectively promoted by about 1.5 to 2.2 times as compared with the control group (see Fig. 3). As a result, as the electrodeposition agent for hairy roots, carboxylmethyl cellulose (CMC) and Arabic gum (AG) were treated and coated first, and perlite and Rhizobium ( Rhizobium sp.) and perlite were mixed with each other by confirming the effect of promoting germination and promoting growth of the soybean and the green grass seeds in the high salt environment soil by treating the strains with a secondary coating by treating the strains of Rhizobium sp. And can be usefully used as a method for promoting germination and promoting growth of soybean and green grass seeds.

Hereinafter, the present invention will be described in detail with reference to examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

< Example  1> Electroplating agent selection and treatment concentration setting

 Seed pellets are used to bond the covering material to the seed surface after the adhesive material is applied to the seed surface. The covering materials are capable of binding to some extent when water is added. However, in transportation or in farming, There is a great deal of concern that the adhesion between the seed and the coating material must be strengthened. Therefore, two kinds of electrodeposition agents such as carboxymethyl cellulose (CMC) and arabic gum (AG) were selected as the hairy vetch seed coating electrodeposition agent, and the hair germination rate In order to set the optimum electrodeposition agent and proper concentration, the following method was performed.

Specifically, the hairy vetch seeds were collected, and the hairy vetch seeds except for the seedlings of the collected seeds were selected, and arabic gum and carboxymethylcellulose as the electrodeposits were selected as 0.5, 1.0, 1.5, 2.0, 2.5 The seeds were dipped in sterilized water for 30 min. Then seeds were placed on a filter paper treated with sterilized water. The germination rate was observed for 4 days in a thermostat at 28 ° C. Seed germination was repeated 3 times The lip / pots were used one by one.

As a result, as shown in Table 1, germination rates of the group treated with arabic gum at the concentrations of 1.0, 1.5 and 2.0% and the group treated with carboxymethylcellulose at the concentrations of 0.5 and 1.0% were similar to those of the untreated group It was confirmed that both of the preforms could be used as an appropriate electrodeposition agent for the hair ribbing seeds. The germination rate (55%) treated with arabic gum 1% and the germination rate (55%) treated with 1% carboxymethyl cellulose were superior to the germination rate (50%) of the untreated group (Table 1). &Lt; tb &gt;&lt; TABLE &gt;

Germination rate (%) Arabic sword
(Arabic gum) (%)
Carboxymethylcellulose
(CMC) (%) No treatment 0.5 One 1.5 2 2.5 0.5 One 1.5 2 2.5 - 48.3 55.0 50.0 50.0 46.7 53.3 55.0 45.0 43.3 43.3 50.0

< Example  2> Clone  Selection

In order to select a hair follicle seed having high stability, the following method was performed.

Specifically, a strain of Rhizobium sp. RHH84 ( Rhizobium sp. RHH84) (KCTC12024BP) having a nodule-forming ability and nitrogen fixation ability isolated from the nadir of Hairy Vicia villosa Roth, After culturing for 3 days in the medium, the culture supernatant was removed and only the cells were recovered. 8 ml of the bacterial suspension at a concentration of 1 × 10 ⁷ cfu / ml was mixed with 20 g of bentonite, kaolin, pyrophylite and perlite and stored at 28 ° C. The bacterial density of Rhizobychna sp. Strain RHH84 was measured for one week.

As a result, as shown in Fig. 1, a suspension of a strain of hairy rhebismus RHH84 ( Rhizobium sp. RHH84) (KCTC12024BP) and a suspension of bentonite, kaolin, pyrophylite, And perlite were mixed, and as a result, the number of viable cells in each preparation was confirmed. As a result, Rhizobium RHH84 ( Rhizobium sp. RHH84) (KCTC12024BP) and pearlite were mixed and the number of viable cells of the bacteria treated was 2 × 10 ⁷ cfu / g, which was about 4 to 20 times higher (FIG. 1).

< Example  3> Confirmation of the stability of coating according to storage time and temperature

Hairy Ribs with Perlite Rectilineus RHH84 ( Rhizobium sp. RHH84) (KCTC12024BP) strain, the following method was performed.

Specifically, a strain of Rhizobium sp. RHH84 ( Rhizobium sp. RHH84) (KCTC12024BP) having a nodule-forming ability and nitrogen fixation ability isolated from the nadir of Hairy Vicia villosa Roth, After culturing in the medium for 3 days, the culture supernatant was removed and only the cells were recovered. 8 ml of the bacterial suspension at a concentration of 1 × 10 ⁷ cfu / ml was mixed with 20 g of perlite and stored for 6 months at different temperature conditions of 4 ° C., room temperature (22 to 23 ° C.) and 28 ° C. The stability of the formulation of pearlite-containing Rhizobia sp. Strain RHH84 was confirmed at intervals of one week.

As a result, as shown in Fig. 2, the pearlite-mixed hair ribbing nerve regrowth RHH84 ( Rhizobium sp. RHH84) (KCTC12024BP) was stored at 4 ° C, room temperature (22-23 ° C) and 28 ° C, respectively. After 3 weeks, the viable cell count was increased, and stable from 4 weeks to 6 months And the viable cell count was maintained. In addition, as a result of confirming the stability of the preparation by temperature, the number of viable cells was similar at 4 ° C and room temperature (22 to 23 ° C), and it was confirmed that the viable cell count was maintained relatively stable even at 28 ° C 2).

< Example  4> Hair Ribechi  Identification of seed germination rate and growth promoting ability

Hair Ribetch Seed Hairy Ribbee Muscle Ribbee Muscle RHH84 ( Rhizobium sp. RHH84) (KCTC12024BP) and perlite were treated in order to confirm the germination rate and growth promoting ability of the hairy vetch seeds.

Specifically, the hairy vetch seeds were selected. In order to sterilize the selected hairy vetch seed surface, it was immersed in 95% ethanol for 1 minute, immersed in 2% sodium hypochlorite (NaClO) for 2 minutes and then washed with sterilized distilled water for 3 to 4 times or more. The washed seeds were dried in a clean bench and used in the present invention. Hair Ribeiro nodule values Lee Jovi ium in RHH84 (Rhizobium sp. RHH84) ( KCTC12024BP) strains and to coat the seeds by using a coating formulation by mixing pearlite (Perlite), Li Jovi ium in RHH84 (Rhizobium sp. RHH84) (KCTC12024BP) was cultured in YMB medium for 3 days, and the culture supernatant was removed and only the cells were recovered. 8 ml of the bacterial suspension having a density of 1 × 10 ⁸ cfu / ml was mixed with 20 g of pearlite, and then coated on the surface of the hairy vetch seed primary coated with 1% CMC electrodeposition. The control group was coated with a sterile pearlite coating agent on the surface of the hairy vetch seed coated with 1% CMC. Seedlings were seeded in pots using the soil of the reclaimed soil with three repeats of hair ripened seedlings coated with the coating agent of the present invention in 10 laps. In January 2013, germination rate and growth promoting ability were observed from 3 weeks to 6 weeks after hairy vetch seed sowing in a greenhouse with a temperature of 20-22 ° C. The soil conditions used in the port experiment were reclaimed soil. The seeds used for sowing were sown with three repeats of 10 lips each of the hair ribbing seeds coated with the coating agent of the present invention.

As a result, as shown in FIG. 3, the hair germination rate of the seeds was found to be 30% after 3 days from the coating and seeding of the hairy vetch seedlings using the coat of the present invention. As a result, Rhizobium RHH84 ( Rhizobium sp. RHH84) (KCTC12024BP) and pearlite (Perlite) treated group, the germination rate of about 70% or more was confirmed. Further, as a result of confirming the growth of hairy vetch on the ground surface, it was confirmed that the growth of the group treated with the hair follicle of the present invention was effectively promoted by about 1.5 to 2.2 times as compared with the control group (Fig. 3).

Korea Biotechnology Research Institute KCTC12024BP 20110928

Claims (13)

Carboxylmethyl cellulose (CMC) and Arabic gum (AG) as electrodeposition agents; And
Soybean and green grass seed coat coatings containing perlite and Rhizobium sp. Strains as coatings.
[Claim 2] The method according to claim 1, wherein the Rhizobium sp. Strain is Rhizobium RHH84 deposited with Accession No. KCTC12024BP sp. RHH84). &Lt; / RTI &gt;
The soybean and green tea seed coating composition according to claim 1, wherein the soybean and green tea crops are any one selected from the group consisting of hairy bean curd, black currant, clover, and nema vermiculite.
[2] The seed coating composition of claim 1, wherein the seed coating agent has an effect of promoting seed germination and promoting growth in a soil of high salt environment.
5. The seed coating composition of claim 4, wherein the high salt environment soil is selected from the group consisting of a reclaimed land, a sea landfill, a vinyl house, and a salt accumulating soil.
The seed coating composition of claim 1, wherein the perlite and the Rhizobium sp. Strain are stored at a temperature of 5 to 30 캜.
The seed coating composition of claim 1, wherein the perlite and the Rhizobium sp. Strain are stored for 5 to 10 months.
i) mixing a carboxylmethyl cellulose (CMC) at a concentration of 0.5 to 2% (v / v) and an Arabic gum (AG) at a concentration of 1-2% (v / v) step; And
( Ii ) a step of mixing the perlite and Rhizobium sp. Strains to prepare a coating agent.
The method according to claim 8, wherein the perlite of step i) and the strain of genus Rizobacterium are mixed in a weight ratio of 1: 1.
The method according to claim 8, wherein the step (ii) comprises 2 × 10 ⁵ cfu / ml to 2 × 10 ¹⁰ cfu / ml.
i) treating the seeds of soybean and green crops with carboxylmethyl cellulose (CMC) and Arabic gum (AG) as an electrodeposition agent and coating them first; And
Ii ) treating the perlite and Rhizobium sp. Strains as a coating to effect a secondary coating, thereby promoting or promoting the germination rate of the soybean and the green grass seeds.
[12] The method according to claim 11, wherein the soybean and green crops are any one selected from the group consisting of hairy vetch, black woongyu, clover, and nema vermilion.
12. The method according to claim 11, wherein the soybean seeds have a germination promoting effect and a growth promoting effect in a soil of high salt environment.

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