WO2013100405A1

WO2013100405A1 - Bacillus subtilis kbc1010 strain, and composition for controlling plant pathogens containing culture broth of same as active ingredient

Info

Publication number: WO2013100405A1
Application number: PCT/KR2012/010162
Authority: WO
Inventors: 강훈석; 강재곤; 백상훈; 박정찬; 한상훈; 박창성
Original assignee: (주)한국바이오케미칼
Priority date: 2011-12-28
Filing date: 2012-11-28
Publication date: 2013-07-04
Also published as: JP2015503548A; KR101184296B1

Abstract

The present invention relates to the Bacillus subtilis KBC1010 strain and to a composition for controlling plant pathogens containing a culture broth of same as an active ingredient. More specifically, the Bacillus subtilis KBC1010 strain, which has been isolated from soil, can be used to advantage as an environmentally friendly composition which: exhibits outstanding antifungal activity against diverse plant pathogens including the turf-damage-inducing pathogen Rhizoctonia solani AG-2-2(IV); is less toxic than existing synthetic organic agrochemicals and does not cause damage to ecosystems, people and animals; and can control organisms pathogenic to turf and other plants.

Description

【Specification】

[Name of invention]

Composition for controlling plant pathogens containing Bacillus subtilis KB C 1 0 1 0 strain and its culture as an active ingredient

Technical Field

The present invention relates to a bacterium subtilis mClO BaciJJus subtilis BC1010) strain isolated from the intestine of slugs inhabiting pine and a composition for controlling grass and other plant pathogens containing the culture medium thereof as an active ingredient.

Background Art

The grass is naturally wild and has a strong resistance to disease and does not develop under normal development. However, under When receiving a stress, such as ⁱ changes in the growing conditions of the intensive management, depression and wound on curlers the onset environment standard normal growth conditions when the management cultivated for special purposes, commercially available, such as a golf course is not a problem There is a tendency to easily develop pathogens.

There are about 14 kinds of diseases that occur on grasses planted in golf courses in Korea, and about 17 kinds of pathogens are known (Korea Society of Plant Pathology, 2009. Korea Plant Disease List). The main disease is brown leaf blight, Skrlotetinia homoekapa (5c / e //), caused by Raiztonia solani age -l (IA) [oc c «/ s solani AG-l (IA)]. y? / 2 homoeocarpa-induced coin blight, Pythium spp.), anemia caused by Plethium blight, colletotricum acuatum b // eo / ^" / cA acutatum) ^] Dried leaf blight, caused by foot stalks of ceratopsia 0 / oc ^ M / a cerea // s, Laytononia solani age -2-2 (IV) [¾ / cii / a solani AG—2-2 ( IV)] is caused by Raiztonia blight (large patch). Various kinds of organic synthetic pesticides are used for the control of the grass disease, but not only the residents around the golf course but also the players are negatively recognized by the problems of ecosystem destruction by pesticide soil residue. Thus, the ^"Development of non-toxic disinfectant as alhwan the environmentally friendly control is urgently required. Various grass disease compositions are being developed using microorganisms applied to agriculture at home and abroad, but compositions having excellent antifungal activity against grass disease are insufficient. Meanwhile, as a composition for controlling plant pathogens using Bacillus subtilis, Korean Patent No. 769360 discloses that the novel Bacillus subtilis S 37-2 strain and microbial fertilizer using the same exhibit antifungal activity against mycobacterium fungi. In Korea Patent No. 825652, the novel Bacillus subtilis Divi 1501 strain discloses antifungal activity against cucumber powdery mildew, strawberry powdery mildew, melon white powdery mildew, peach bacterial pore disease, and ginseng spot pattern disease, and is registered in Korea. Patent No. 890013 discloses a novel Bacillus subtilis cage 1 and a microbial pesticide using the same exhibit antifungal activity against pepper blight. In addition, Korean Patent No. 1093579 discloses a novel thermophilic Bacillus subtilis D.O.K. 0327 strain exhibiting antifungal activity against lettuce fungal nucleus disease, but Laytononia solani age-2, a pathogen causing grass disease. -2 (ΐν) [^ σ £? Ω / 3 solani AG-2-2 (IV)] is a composition for controlling pathogens using microorganisms that exhibits strong antifungal activity against lysoneonia blight (large patch) None known.

Accordingly, the present inventors endeavored to develop a composition having excellent antifungal activity against a variety of plant pathogens, Bacillus subtilis KBC1010O¾c /// ws subtilis KBC1010) strain and its culture solution deposited with accession number KCTC 10304BP to the grass disease A variety of pathogens, including the causative agent Lyraitonia solani age-2-2 (IV) / ^ c OT / a solani AG ᅳ 2-2 (IV) _. Excellent antifungal activity against plant pathogens The present invention was completed by confirming the appearance.

[Detailed Description of the Invention]

[Technical problem]

Object of the present invention is Bacillus subtilis deposited under accession number KCTC 10304BP

It is to provide a composition for controlling grass and other plant pathogens containing KBC1010 (^ 3c /// £ / 5 subtil is KBC1010) strain and its culture as an active ingredient, and a method for producing the same.

In addition, another object of the present invention is to provide a Bacillus subtilis KBC1010 strain deposited with Accession No. KCTC 10304BP, and a culture for controlling grass lysonia blight (large patch) containing the culture medium thereof as an active ingredient.

Technical Solution

In order to achieve the above object, the present invention provides a composition for controlling plant pathogens containing Bacillus subtilis KBC1010 (fec / / / i / s subtilis KBC1010) strain and the culture solution thereof deposited as Accession No. KCTC 10304BP. to provide.

In addition, the present invention provides a Bacillus subtilis mCm0 (Bac / Hus subtil Is KBC1010) strain deposited with Accession No.KCTC 10304BP and a culture for controlling grass lysogenic blight (large patch) containing the culture medium thereof as an active ingredient. do.

In addition, the present invention

1) preparing a culture by culturing Bacillus subtilis KBC1010G¾c /// i / s subtilis KBC1010) strain deposited with accession number KCTC 10304BP in a liquid medium; And

2) It provides a method for producing a plant pathogen control composition comprising the step 1) drying and powdering the prepared culture to prepare a raw agent.

Advantageous Effects

Bacillus subtilis KBC1010 (^ C /// / ^ /// sKBC1010) strain of the present invention or Its cultures have excellent antifungal activity against a variety of plant pathogens, including the lysatonia solani age -2-2 (1 V) oc iw / a solani AG-2-2 (IV), a pathogen causing grass disease. Therefore, it can be usefully used as an environment-friendly composition for controlling plant pathogens.

[Brief Description of Drawings]

FIG. 1 is a diagram showing the inhibitory zones generated after replacement of various phytopathogens and Bacillus subtilis KBC1010 (fec /// "s subtilis KBC1010):

1) The Botrytis Ceria 05b o ^ / 5 cinerea ^ Bacillus subtilis

Figure shows lowland generated after replacement of KBC1010;

2) is a diagram showing the lowland produced after the incubation of Lysatonia serearis (/ ^: (/ a ce / "ea // s) and Bacillus subtilis KBC1010;

3) is a diagram showing the lowland produced after the incubation of Choletotricum accutum // ^ 0 / ^ acutatum) Bacillus subtilis KBC1010;

4) is a plot showing the lowland produced after the incubation of Wer / rolfsii ^ Bacillus subtilis KBC1010;

5) is a diagram showing the lowland produced after the incubation of Lysatonia Solani Age-l (IA) [7 / oc «7 / a solani AG-l (IA)] and Bacillus subtilis KBC1010; And 6) is a diagram showing the lowland generated after replacement of Bacillus subtilis KBC1010] with Raiztonia solanie age-2—S ROI ^ / ^ cii / a so / / AG-2-2CIV). . Figure 2 is a diagram confirming the effect of the control of grass Lyriatonia blight (large patch) of Bacillus subtilis KBC1010 strain through a field experiment. [Form for implementation of invention]

Hereinafter, the present invention will be described in detail. The present invention provides a composition for controlling plant pathogens containing Bacillus subtilis mCl MBac / Jlus subtilis KBC1010) strain deposited with Accession No. KCTC 10304BP or a culture thereof as an active ingredient.

In addition, the present invention provides Bacillus subtilis KBC1010G¾c /// us subtilis KBC1010; Accession No. KCTC 10304BP) Provides a method for controlling plant pathogens, comprising the step of treating a plant or soil with a strain or a culture thereof.

In addition, the present invention is Bacillus subtilis KBC1010G¾c / / / is subtilis KBC1010; Accession No. KCTC 10304BP) strain or a culture thereof is provided for use in a composition for controlling plant pathogens.

In addition, the present invention is a medium containing Bacillus subtilis KBC1010 (^ c / / s subtilis KBC1010; accession number KCTC 10304BP) strain 0.1 to 10.0 parts by weight of dextrose and 0.1 to 10.0 parts by weight of peptone The culture obtained by incubating at 25 to 35 ⁰ C and pH 5.5 to 8.5 in a dry and powdered composition containing 5.0 to 50.0% (w / w) of the original agent, and an extender, 200 to 1000 times dilution in water It provides a method for controlling plant pathogens comprising the step of irrigation of ¹ L / m ² of soil.

The present invention also provides a Bacillus subtilis KBC1010 (^ c /// s subtilis KBC1010; accession number KCTC 10304BP) strain containing 0.1 to 10.0 parts by weight of dextrose and 0.1 to 10.0 parts by weight of peptone. Use of a composition comprising a raw material 5.0 to 50.0% (w / w) dried and powdered by culturing at 25 to 35 ° C. and pH 5.5 to 8.5 at 25, and an extender, in a hydrous composition for controlling plant pathogens To provide.

The Bacillus subtilis BC1010 strain was deposited by the present inventors with accession number KCTC 10304BP to the Korea Biotechnology Research Institute Gene Bank on July 18, 2002. The plant pathogens are Lyaxtonia solani Age-2-2 (IV) [y / zoc o7 / a solani AG-2-2 (IV)], Laytononia serearis / ^ 9C (/ a cerea // s ), Raiztonia Solani Age -l (IA) [y¾ oc (/ a solani AG-l (IA)], Botrytis Ceria (? R ᅳ / s cinerea), Colletotricum Accuatum // eor / acutatum) and sctelotium It is preferable that at least one selected from the group consisting of ruff e (r / e / / rolfsi, lyzania solani age-2-2 (0 [^ ¾ / ^ 0/7/350/3/2 / 八 ( More preferably, it is? 2-2.

In a specific embodiment of the present invention, the present inventors confirmed the growth inhibitory effect on plant pathogens of the Bacillus subtilis KBC1010 strain, the bacillus subtilis KBC1010 strain of the present invention has a significant antifungal effect against six plant pathogens it was confirmed that the indicative, especially grass Rhizoctonia blight (large ^patches), pathogen Rhizoctonia solani Age -2 eu 2 (1 \ [¾ inducing / ^? 0/7/3 solani AG-2- 2 (IV)] was confirmed to exhibit a significant antifungal effect (see Table 1 and Fig. 1).

1) preparing a culture by culturing Bacillus subtilis KBC1010 (fec /// "s subtil is KBC1010) strain deposited with accession number KCTC 10304BP in a liquid medium; and

The medium of step 1) preferably includes 0.1 to 10.0 parts by weight of dextrose and 0.1 to 10.0 parts by weight of peptone, but is not limited thereto.

The culture of step 1) is preferably incubated at 25 to 35 ° C and pH 5.5 to 8.5, but is not limited thereto.

Drying and powdering of step 2) is preferably powdered culture medium using a spray dryer or a lyophilizer, but is not limited thereto.

The composition may include the strain or its culture as an active ingredient. The composition according to the present invention may be formulated for controlling plant pathogens in a conventional manner and may be prepared in dry powder form or in liquid form.

Specifically, the composition according to the present invention can be prepared in the form of a liquid biopesticide It may be used in the form of powder powder by adding an extender or granulated by formulating it. However, the formulation is not particularly limited.

In a specific embodiment of the present invention, the present inventors, after liquid culture of the strain of the present invention, to prepare a powder raw material, and then examined the number of live spores according to the silver and pH of the culture medium and powder raw material, 1.0xl0 ⁹ cfu / ml and 1.0xl0 ⁹ cfu / g or more content was confirmed to show a similar productivity to the general Bacillus strains (see Table 2 and Table 3).

In addition, the inventors of the present invention was formulated into a hydrating agent by mixing the Bacillus subtilis KBC1010 strain of the present invention and the extender, etc., and then examined the physicochemical properties and the amount of spores, the hydration of the present invention is good powder degree and hydration It was confirmed that it has a significant amount of spores (see Table 4).

The extender added to the composition serves to control the amount of the total biopesticide composition to be contained in an appropriate ratio in an active ingredient composition such as microorganisms. Extenders that can be used in the present invention include sodium alginate, gelatinized starch, corn starch, soybean meal, wheat flour, granular fiber, yuan, diatomaceous earth, zeolite, bentonite, talc, kallin, pyrophyllite, white carbon, sugar, etc. This can be used.

As described above, the Bacillus subtilis KBC1010 (^ c // 7i / 5 s KBC1010) strain deposited with KCTC 10304BP of the present invention and a composition containing the culture medium thereof as an active ingredient may be prepared in various formulations. Since various formulations contain significant number of live spores, the pathogens that cause turf disease are Lyzatonia Solani Age-2-2 (IV) [7¾ / oc o? / A solan / AG-2-2 (IV). ] Can be usefully used as a composition for the control of environmentally friendly plant pathogens for various plant pathogens. The control method using the composition for controlling plant pathogens is generally performed in general, that is, spraying (for example, spraying, misting, atomizing, powder spraying, granulating spraying, sleeping, permanent use, etc.), soil application (for example, For example, it may be carried out by shaking, irrigation, etc.), surface use (for example, coating, smearing, coating, etc.), dipping, or smoking. That The amount used may be appropriately determined according to the damage situation, the method of application, and the place of application. In addition, the present invention provides a composition for the control of grass lysonia blight (large patch) containing Bacillus subtilis mC O (Bac / Hus subtilis KBC1010) strain or culture medium thereof deposited with KCTC 10304BP as an active ingredient.

In addition, the present invention is a Bacillus subtilis KBC1010 (fec / / / ws subtilis KBC1010; accession number KCTC 10304BP) strains or cultures thereof comprising the step of treating the plant or soil Laytonia solani age -2-2 ( IV) Provide a method of controlling turf Laytononia blight (large patch) caused by age.

In addition, the present invention is Bacillus subtilis KBC10100¾c / / s subtilis KBC1010; Accession No. KCTC 10304BP) strain or culture thereof

Provided for use in a composition for controlling grass lyxtonian blight (large patch) caused by age-2-2 (IV).

The turf Raiztonia blight (large patch) is the most common disease in Korean golf grass in Korea, Raiztonia Solani Age-2-2 (IV) U / oc iM / a solani AG-2-2 (IV )] Is caused by the pathogen, it was confirmed that the composition of the present invention exhibits a control effect against remarkable grass Laytononia blight (large patch) (see Fig. 2 and Table 5). ,

Therefore, Bacillus subtilis mClOlOiBaciJJus subtilis KBCIOIO strains deposited with Accession No. KCTC 10304BP of the present invention and cultures thereof are lysatonia solani age-2-2 (IV) / ^ c cw / 3 solani AG-2-2 (IV)] Since it exhibits excellent antifungal activity against the pathogen-induced grass lyxtonia blight (large patch), it can be usefully used as an active ingredient for controlling the composition of grass lyxtonia blight (large patch). Hereinafter, the present invention will be described in detail by way of examples.

However, the following Examples are specifically illustrated the present invention, the contents of the present invention It is not limited by the Example.

Example 1 Confirmation of Growth Inhibitory Effect on Plant Pathogens

The present inventors cultured various plant pathogens and Bacillus subtilis KBC1010 on sterilized potato agar medium to confirm the growth inhibitory effect on plant pathogens of Bacillus subtilis BC1010 deposited with accession number KCTC 10304BP. At this time, the plant pathogen is in potato agar medium. Subcultured subpopulations were circular sections of 5 mm in diameter and inoculated using Bacillus subtilis KBC1010 cultured in LB medium. Then, after incubating for 10 days at 28 ° C. temperature of the growth of various pathogens, the diameter of the inhibition zone (Inhibition zone) was measured.

Examples of the plant pathogens include grass plant pathogens that cause a lot of damage to crops, such as lyc trioni solani age-2-2 (IV) / zoc / a solani AG-2-2 (IV)] ¾7 o cM / a cereal is), Lysatonia solani -lGA) [/ 7 oc ^ v? / A solani AG-l (IA)], and other common plant pathogens. ^ / s cinerea), Collet sat tree glutamicum ^{0 \ ^^^ Col let otri chum acutatum} ) and Micrel's Loti help ^ peusyo Sclewthun was used rolfsii ^ 6 species.

As a result, as shown in Figure 1 and Table 1, it was confirmed that the Bacillus subtilis KBC1010 strain exhibits a significant antifungal effect against all of the plant pathogens, in particular, Laytononia Solani, a pathogen that causes grass disease It was confirmed that the age -2-2 (IV) [^ / ^ 3c OT / a solani AG-2-2 (IV)] exhibits significant antifungal activity (Table 1 and Figure 1).

Table 11

Antifungal Spect of Bacillus subtilis KBC1010 Strain ¾

+: 0 <X ≤ 2 讓

++ ： 2mm <X ≤ 5 顏

+++ ： 5ιιιιη <X <lOmni

++++ ： 10mm <X <15 匪

+++++ ： 15 讓 <X

Example 2 Liquid Culture of Bacillus Subtilis KBC1010 Strain and Preparation of Powder Raw Material The Bacillus subtilis KBC1010 strain of the present invention was subjected to liquid culture using a 1.2KL incubator.

Specifically, Bacillus subtilis KBC1010 strain in a liquid medium containing dextrose 0.01-10.0% and peptone 0.01-10.0% as a medium component (w / v) ⁰ C, pH 5.5 ~ 8.5 was adjusted to incubation using 1.2KL fermenter. In addition, the total incubation time was 48 hours, and after the end of the culture, the contents of the live spores according to the temperature and pH of the culture medium, and the sprayed and lyophilized powder raw materials, were all 1.0x l0 ⁹ cfu / ml and 1.0 By showing a content of x l0 ⁹ cfu / g or more was confirmed to show a similar productivity to the general Bacillus strains (Table 2 and Table 3).

Table 2

Spore Number Contents according to Temperature and pH of Bacillus Subtilis KBC1010 Strain Cultures and Spore Numbers of KBC1010 Cultures, Dry Powder and Freeze-Dried Ingredients

[Table 3]

Example 3 Preparation of a Composition Containing Bacillus Subtilis KBC1010

Bacillus subtilis KBC1010 strain prepared in <Example 3> 5.0 to 50.0% (w / w) of the original agent and 50.0 to 95.0% (w / w) extender was mixed and then prepared a hydration formulation. As a result of formulating with a hydrating agent as described above, the hydrating formulation of the present invention showed a good powder degree and hydration properties disclosed in RDA criterion "Testing method of pesticide", the Bacillus subtilis KBC1010 spores content in the hydrating agent is 5.1xl0 ⁷ cfu / g or more was confirmed to have a suitable effective content (Table 4). Table 4

Physicochemical Properties and Microbial Content of Bacillus Subtilis KBC1010 Hydration

Example 4 Plant Pathogen Control Effect of Bacillus subtilis KBC1010

In the early stages of the development of turf Lai-tononia blight (large patch) in turfgrass (variety: Korean grass) in Gyeongnam, the water-repellent agent of the present invention prepared in Example 3 was diluted 200-1000 times in water and 1 L. Soil irrigation was performed once at / m ² (August 30, 2011). After 9, 21 days and one month after the last drug treatment, photographs were taken of the grass-laytonnia blight (large patch) test group.

As a result, as shown in Fig. 2 and Table 5, KBC1010 hydrating agent of the present invention showed a high control value of up to 89.4> and confirmed that the practicality as a biopesticide is high (Fig. 2 and Table 5).

[Table 5]

Effect of Hydration of the Present Invention on Grass Laytononia Blight Disease (Large Patch)

Industrial Applicability Bacillus subtilis KBC1010 strain of the present invention and a composition for the control of plant pathogens containing the culture medium thereof as an active ingredient is a pathogen causing grass disease Lycania solani age -2-2 (IV) [¾ / oc OT / a solani AG-2-2 (IV)] shows excellent antifungal activity against various plant pathogens, including environmentally friendly composition for controlling plant pathogens against various plant diseases including grass lytononia blight (large patch). It can be usefully used. Therefore, it is possible to solve the destruction of ecosystems and human toxicity caused by chemical pesticides can be very useful in the pesticide industry and environmental industry.

[Accession number]

Depositary Name ： Korea Research Institute of Bioscience and Biotechnology

Accession number ： KCTC10304BP

Trust date: 20020720

Burned station door

Budapest Treaty on International Approval of Microbial Deposits in Patent Procedure

International format

According to rule 7.1

Receipt of Won Deposit

O ： Shin

Korea Bio Chemical Co., Ltd.

# 1539-6, Dongdong-ri, Uiryeong-eup, Uiryeong-gun, Gyeongsangnam-do, South Korea (636-803)

BP / 4 type (KCTC type 17)

Claims

[Range of request]

[Claim 11

Bacillus subtilis KBC1010 ^' (fec / / / i / s subtilis KBC1010; accession number KCTC 10304BP) strain or a composition for the control of plant pathogens containing the culture medium thereof as an active ingredient.

[Claim 2]

The plant pathogen of claim 1, wherein the plant pathogen is Lyxatoni solani age ^^ (IV ^^ / oc i / s solani AG-2-2 (IV)], Lyxatoni serearis (7 / oc i / 3 cereal is), Lysatonia Solani Age -l (IA) [/ / zoc »? / a solani AG-KIA)], Botrytis Ceria C? ry / s cinerea), cholettotrium c> ^^^ ColletotrichLun acutatum) and Sterlotium lfsi (5Wero / ffl rolfsii) —A composition for controlling plant pathogens, characterized in that at least one selected from the group consisting of.

[Claim 3]

Turf Laytononia blight caused by Bacillus subtilis KBC1010 (fec // s subtilis KBC1010; Accession No. KCTC 10304BP) strain or its culture solution as an active ingredient Raiztonia Solani Age— 2-2 (IV) (Large patch) Composition for controlling.

[Claim 4]

1) preparing a culture by culturing Bacillus subtilis KBC1010 (^ c /// ws subtilis KBC1010) strain deposited with accession number KCTC 10304BP in a liquid medium; And 2) The method of producing a plant pathogen control composition comprising the step 1) preparing a raw material by drying and powdering the prepared culture.

[Claim 5]

The method of claim 4, wherein the medium of step 1) comprises 0.1 to 10.0 parts by weight of textrose (dextrose) and 0.01 to 10.0 parts by weight of peptone (peptone) of the production of a composition for controlling plant pathogens Way .

[Claim 6]

The method of claim 4, wherein the culturing of step 1) is carried out at 25 to 35 ^° C and pH 5.5 to 8.5.

[Claim 7]

After mixing the strain agent 5.0 to 50.0¾> (w / w) and the extender 50.0 to 95.0% (w / w) prepared by the method of claim 4 to 6, characterized in that it is prepared by adding water Formulations for Plant Pathogen Control.

[Claim 8]

A method for controlling plant pathogens, comprising diluting the formulation for controlling plant pathogens of claim 7 in water 200-1000 times in lL / m ² .

[Claim 9]

Bacillus subtilis KBC1010 (fec /// i / s subtilis KBC1010; accession number KCTC

10304BP) dry the culture obtained by incubating the strain at 25 to 35 ° C. and pH 5.5 to 8.5 in a medium containing 0.11 to 10.0 parts by weight of dextrose and 0.01 to 10.0 parts by weight of peptone And powdered raw agent 5.0 to 50. o (w / w), and a hydrating composition for plant pathogen control, characterized in that the soil irrigation of lL / m ² by diluting the composition containing 200 to 1000 times in water .

[Claim 10] 10. The plant pathogen of claim 9, wherein the plant pathogen is Rhizoctonia solani AG-2-2 (IV), Rhizoctonia cerealis, or Ryazotonia solar. Any one selected from the group consisting of Rhizoctonia solani AG-l (IA), Collet ot rich 醒 acutatum and Sclerotium rolfsii Hydration composition for plant pathogen control characterized by the above.

[Claim 11]

10. The method of claim 9, wherein the extender comprises sodium alginate, gelatinized starch, corn starch, soybean meal, bran, granular fiber, yuan, diatomaceous earth, zeolite, bentonite, talc, kaolin, pyrophyllite, white carbon and sugars. Hydrating composition for controlling plant pathogens, characterized in that any one selected from the group.

[Claim 12]

Bacillus subtilis KBC1010 (fec /// t / s subtilis KBC1010; accession number KCTC

10304BP) A method for controlling plant pathogens, comprising the steps of treating a plant or soil with a strain or a culture thereof.

[Claim 13]

Bacillus subtilis KBC1010 (fec / 7 / i / s subtilis KBC1010; accession number KCTC

10304BP) A method for controlling turf Laytononia blight (large patch) caused by Laytononia Solani Age-2-2 IV) comprising the step of treating a strain or a culture thereof to a plant or soil.

[Claim 14]

Bacillus subtilis KBClOlOCfec /// ^ subtilis KBC1010; Accession No. KCTC 10304BP) strain of dextrose 0.1 to 10.0 parts by weight and peptone 0.1 A composition comprising 5.0 to 50.0¾> (w / w) of an original agent, which was dried and powdered from a culture obtained by culturing at 25 to 35 ° C. and pH 5.5 to 8.5 in a medium containing 1 to 10.0 parts by weight, and an extender, Diluting 200 to 1000 times in the soil plant control method comprising the step of irrigation of the soil lL / m ² .

[Claim 15]

Use of Bacillus subtilis KBC1010 (^ c /// i / s subtil is KBC1010; Accession No. KCTC 10304BP) strain or a culture thereof in a composition for controlling plant pathogens. [Claim 16]

Bacillus subtilis KBC1010 (fec /// z / 5 subtil is KBC1010; accession number KCTC 10304BP) strains or their cultures were treated with Laitononia Solani age-2-2 (IV); Large patch) Use for control composition. [Claim 17]

Bacillus subtilis KBC1010O¾c /// i «subtil is KBC1010; Drying the culture obtained by incubating the Accession No. KCTC 10304BP strain in a medium containing 0.1-10.0 parts by weight of dextrose and 0.1-10.0 parts by weight of peptone at 25-35 ^° C. and pH 5.5-8.5 Use of the composition containing the powdered raw agent 5.0-50.0% (w / w), and an extender for the hydration composition for plant pathogen control.