CN110037057A - A kind of broad-spectrum micro-organisms pesticide and preparation method thereof - Google Patents
A kind of broad-spectrum micro-organisms pesticide and preparation method thereof Download PDFInfo
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- CN110037057A CN110037057A CN201910448792.1A CN201910448792A CN110037057A CN 110037057 A CN110037057 A CN 110037057A CN 201910448792 A CN201910448792 A CN 201910448792A CN 110037057 A CN110037057 A CN 110037057A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
- A01N39/02—Aryloxy-carboxylic acids; Derivatives thereof
- A01N39/04—Aryloxy-acetic acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Abstract
A kind of broad-spectrum micro-organisms pesticide and preparation method thereof, the present invention relates to technical field of pesticide；It includes following parts by weight ingredient: 0.8-1.0 parts of beauveria bassiana, 0.6-1.0 parts of Paecilomyces lilacinus, 0.8-1.3 parts of Trichoderma harzianum, 8-12 parts of expanded perlite, 1-3 parts of sodium carboxymethylcellulose, 2-4 parts of starch, 6-8 parts of diatomite, 7-12 parts of flyash, 15-30 parts of straw powder, 3~5 parts of acrylamide, 5~8 parts of xylitol, 9-13 parts of clay, 3-5 parts of plant growth regulating substance, 3-6 parts of composite synergist, 27.9~38.5 parts of water.Have the function of the prevention of wide spectrum and prevention and treatment diseases and pests of agronomic crop; the control efficiency having had to harmful organisms such as grub, cutworm, garlic maggot, bradysia odoriphaga, root-knot nematode, tomato late blight, capsicum late blight, cucumber fusarium axysporums; and it is stable product quality, efficient, nontoxic; it is safe to the human body; it is pollution-free; it preserves the ecological environment, energy substituting chemical pesticide is used for agricultural production, adapts to the demand for development of " green agriculture ".
Description
Technical field
The present invention relates to technical field of pesticide, and in particular to a kind of broad-spectrum micro-organisms pesticide and preparation method thereof.
Background technique
Capsicum epidemic disease, cucurbits fusarium wilt, damping-off, graw mold of tomato, root-knot nematode, grub, cutworm, garlic maggot, bradysia odoriphaga
Etc. being important soil-borne disease and subterranean pest-insect.Beauveria bassiana single dose is to subterranean pest-insects such as grub, garlic maggot, bradysia odoriphaga, cutworms
Control efficiency it is preferable；Paecilomyces lilacinus single dose has preferable control efficiency to root-knot nematode etc.；Trichoderma harzianum single dose is to tomato
The important soil-borne disease control efficiency such as gray mold, cucurbits fusarium wilt is preferable.
In vegetables production, root-knot nematode, soil-borne disease, subterranean pest-insect harm are serious, often result in 10%~30% underproduction,
When serious generation, up to 50% or more, individual plot even cause to have no harvest for production loss.These harmful organisms not only cause to subtract
It produces, while substantially reduces victual quality.Currently, mostly using chemistry greatly to the prevention and treatment of these important harmful organisms in production
Medicament, production cost is higher, and is also easy to produce drug resistance and larger to environmental toxicity, causes environmental pollution, seriously threatens people and animals
Health.
Currently, microorganism selected by most microbial pesticides is single or less, prevention spectrum is relatively narrow, and exists and use
In the process the problem of quality less stable, using effect is not ideal enough in the specifically practice of prevention and treatment pest and disease damage, in harmful organism
The deficiencies of when carrying out Synthetical prevention, it is more that there are times for spraying, and input cost is big, inconvenient place.
Preventing and treating substitution chemical pesticide control using microbial pesticide is that current chemical pesticide subtracts one of the Critical policies applied, will
There is a variety of pairs of pest and disease damages the microbial bacterial agent compounding of preferable control efficiency to be paid more and more attention for harmful organism Synthetical prevention.
Suitable biocontrol bacterial strain is selected, and carries out the matched combined of science, it is wider to the prevention spectrum of harmful organism, and can effectively keep away
Exempt from the generation of harmful organism drug resistance, protect pest natural enemy, keep the sustainable development of ecological environment, anticipates with important development
Justice.
Summary of the invention
In view of the defects and deficiencies of the prior art, the present invention intends to provide a kind of structure is simple, design rationally, make
With convenient broad-spectrum micro-organisms pesticide and preparation method thereof, have the function of the prevention of wide spectrum and prevention and treatment diseases and pests of agronomic crop, it is right
The harmful organisms such as grub, cutworm, garlic maggot, bradysia odoriphaga, root-knot nematode, tomato late blight, capsicum late blight, cucumber fusarium axysporum have
Good control efficiency, and it is stable product quality, efficient, nontoxic, it is safe to the human body, it is pollution-free, it preserves the ecological environment, it can substitutionization
Medicine of learning to farm is used for agricultural production, adapts to the demand for development of " green agriculture ".
To achieve the above object, the broad-spectrum micro-organisms pesticide in the present invention, it includes following parts by weight ingredient: the white deadlock of ball spore
0.8-1.0 parts of bacterium, 0.6-1.0 parts of Paecilomyces lilacinus, 0.8-1.3 parts of Trichoderma harzianum, 8-12 parts of expanded perlite, carboxymethyl cellulose
Plain sodium 1-3 parts, 2-4 parts of starch, 6-8 parts of diatomite, 7-12 parts of flyash, 15-30 parts of straw powder, 3~5 parts of acrylamide, wood
5~8 parts of sugar alcohol, 9-13 parts of clay, 3-5 parts of plant growth regulating substance, 3-6 parts of composite synergist, 27.9~38.5 parts of water.
Further, the partial size of the flyash is 50~200 μm, and porosity is 60%~80%.
Further, the grain diameter of the straw powder is 200~500 μm, carbohydrate content 40%~
50%.
Further, the plant growth regulating substance is heteroauxin, 2,4-D, methyl α-naphthyl acetate, gibberellin, brassin
One or more of lactone, cycocel, sodium humate, paclobutrazol mix.
Further, the composite synergist include amino acid, borax, zinc sulfate, ammonium nitrate, chitosan, Tea Saponin,
Ferrous sulfate, copper sulphate, manganese sulfate, ammonium molybdate, in urea at least five kinds mix.
Further, the beauveria bassiana, Paecilomyces lilacinus, strain content is 80~10,000,000,000 in Trichoderma harzianum
A spore/mL.
The preparation method of broad-spectrum micro-organisms pesticide in the present invention, its step are as follows:
1, the preparation of Biocontrol microorganism opportunistic pathogen powder:
1.1, the preparation of beauveria bassiana: by the strain inoculated of the beauveria bassiana of preservation in PDA culture medium, 28 DEG C of perseverances
After temperature culture 2-4d, the culture dish that bacterial strain grows fine is chosen in sterile working, and taking diameter in colony edge is 5mm pure culture biscuits involvng inoculation
In the conical flask equipped with 50mL optimization Cha Shi liquid medium through sterilizing in advance, at 28 DEG C, 150r/min shaken cultivation
It after 7-8d, is transferred in solid fermentation culture by 10% inoculum concentration, mixes, be placed in constant temperature incubation in 28 DEG C of constant incubators
10d or so；Take out fermentation material, 35 DEG C of baking 36h in baking oven；Pulverizer crushes, and crosses 200 meshes to get opportunistic pathogen powder, 4 DEG C of preservations are arrived
It is spare；
1.2, by the strain inoculated of the Paecilomyces lilacinus of preservation in PDA culture medium, 28 the preparation of Paecilomyces lilacinus: are placed in
After cultivating 5-6d in DEG C incubator, choose the culture dish that bacterial strain grows fine, colony edge take diameter be 5mm pure culture biscuits involvng inoculation in
In PDB culture solution, 28 DEG C, 150r/min shaken cultivation 5d are transferred in solid fermentation culture by 10% inoculum concentration, are mixed,
It is placed in constant temperature incubation 10d or so in 28 DEG C of constant incubators；Take out fermentation material, 35 DEG C of baking 36h in baking oven；Pulverizer crushes, mistake
To get opportunistic pathogen powder is arrived, 4 DEG C save backup 200 meshes；
1.3, the preparation of Trichoderma harzianum: the trichoderma harzianum strain of preservation is seeded in PDA culture medium, 28 DEG C, culture 3-
After 4d, the culture dish that bacterial strain grows fine is chosen, taking diameter in colony edge is 5mm pure culture biscuits involvng inoculation in PD culture solution, 28 DEG C,
It after 150r/min shaken cultivation 5d, is transferred in solid fermentation culture by 10% inoculum concentration, mixes, be placed in 28 DEG C of constant temperature incubations
Constant temperature incubation 10d or so in case；Take out fermentation material, 35 DEG C of baking 36h in baking oven；Pulverizer crushes, and crosses 200 meshes to get original is arrived
Bacterium powder, 4 DEG C save backup；
2, according to mass ratio 1:1:3:4:5:16 sodium carboxymethylcellulose, starch, diatomite, flyash, expanded pearlite
Rock and water are mixed and stirred for uniformly, obtaining absorption mixture, this absorption mixture is aseptically placed 4d；
3, the opportunistic pathogen powder of beauveria bassiana, Paecilomyces lilacinus, Trichoderma harzianum, absorption mixing are added in absorption mixture
Object: beauveria bassiana: Paecilomyces lilacinus: the ratio of Trichoderma harzianum is 50:1:1:1, obtains the mixture of absorption microorganism；
4, according to mass ratio 1:2:3:5:1:1 acrylamide, xylitol, clay, straw powder, Plant growth regulators
Matter, composite synergist are mixed evenly, and obtain Synergistic blend；
5,1:1 in mass ratio is uniformly mixed the mixture and Synergistic blend of absorption microorganism, obtains compounding microorganism
Pesticide.
After adopting the above structure, the invention has the following beneficial effects: a kind of broad-spectrum micro-organisms pesticide of the present invention and its system
Preparation Method will be mainly used for the beauveria bassiana for preventing and treating subterranean pest-insect (grub, cutworm etc.), the pale purple of prevention and treatment root-knot nematode is intended
Mould and prevention and treatment soil-borne disease fungal pathogens trichoderma harzianum compounding mixing, carry out integrated control, pesticide to the harmful organism in soil
In the straw powder containing larger proportion, while can also increase the content of organic matter of soil, play certain improvement soil texture
Effect；Have the function of wide spectrum prevention with prevention and treatment diseases and pests of agronomic crop, to grub, cutworm, garlic maggot, bradysia odoriphaga, root-knot nematode,
The control efficiency that the harmful organisms such as tomato late blight, capsicum late blight, cucumber fusarium axysporum have had, and stable product quality, height
It imitates, is nontoxic, it is safe to the human body, it is pollution-free, it preserves the ecological environment, energy substituting chemical pesticide is used for agricultural production, adapts to " green agriculture
The demand for development of industry ".
Specific embodiment
Broad-spectrum micro-organisms pesticide in present embodiment (embodiment one), it includes following parts by weight ingredient: ball spore
0.8 part of muscardine, 0.8 part of Trichoderma harzianum, 10 parts of expanded perlite, 2 parts of sodium carboxymethylcellulose, is formed sediment at 0.8 part of Paecilomyces lilacinus
2 parts of powder, 8 parts of diatomite, partial size are 200 μm, and 10 parts of the flyash that porosity is 80%, grain diameter are 200 μm, carbon hydrate
Object content be 45% 20 parts of straw powder, 4 parts of acrylamide, 8 parts of xylitol, 12 parts of clay, 4 parts of plant growth regulating substance,
4 parts of composite synergist, 32 parts of water.
Further, the plant growth regulating substance be gibberellin, it is brassin lactones, cycocel, sodium humate, more
It is mixed in effect azoles.
Further, the composite synergist includes ammonium nitrate, chitosan, Tea Saponin, ferrous sulfate, copper sulphate, sulphur
Sour manganese, ammonium molybdate, urea mix.
Further, the beauveria bassiana, Paecilomyces lilacinus, strain content is 80~10,000,000,000 in Trichoderma harzianum
A spore/mL.
The preparation method of broad-spectrum micro-organisms pesticide in present embodiment, its step are as follows:
1, the preparation of Biocontrol microorganism opportunistic pathogen powder:
1.1, the preparation of beauveria bassiana: by the strain inoculated of the beauveria bassiana of preservation in PDA culture medium, 28 DEG C of perseverances
After temperature culture 2-4d, the culture dish that bacterial strain grows fine is chosen in sterile working, and taking diameter in colony edge is 5mm pure culture biscuits involvng inoculation
In the conical flask equipped with 50mL optimization Cha Shi liquid medium through sterilizing in advance, at 28 DEG C, 150r/min shaken cultivation
It after 7-8d, is transferred in solid fermentation culture by 10% inoculum concentration, mixes, be placed in constant temperature incubation in 28 DEG C of constant incubators
10d or so；Take out fermentation material, 35 DEG C of baking 36h in baking oven；Pulverizer crushes, and crosses 200 meshes to get opportunistic pathogen powder, 4 DEG C of preservations are arrived
It is spare；
1.2, by the strain inoculated of the Paecilomyces lilacinus of preservation in PDA culture medium, 28 the preparation of Paecilomyces lilacinus: are placed in
After cultivating 5-6d in DEG C incubator, choose the culture dish that bacterial strain grows fine, colony edge take diameter be 5mm pure culture biscuits involvng inoculation in
In PDB culture solution, 28 DEG C, 150r/min shaken cultivation 5d are transferred in solid fermentation culture by 10% inoculum concentration, are mixed,
It is placed in constant temperature incubation 10d or so in 28 DEG C of constant incubators；Take out fermentation material, 35 DEG C of baking 36h in baking oven；Pulverizer crushes, mistake
To get opportunistic pathogen powder is arrived, 4 DEG C save backup 200 meshes；
1.3, the preparation of Trichoderma harzianum: the trichoderma harzianum strain of preservation is seeded in PDA culture medium, 28 DEG C, culture 3-
After 4d, the culture dish that bacterial strain grows fine is chosen, taking diameter in colony edge is 5mm pure culture biscuits involvng inoculation in PD culture solution, 28 DEG C,
It after 150r/min shaken cultivation 5d, is transferred in solid fermentation culture by 10% inoculum concentration, mixes, be placed in 28 DEG C of constant temperature incubations
Constant temperature incubation 10d or so in case；Take out fermentation material, 35 DEG C of baking 36h in baking oven；Pulverizer crushes, and crosses 200 meshes to get original is arrived
Bacterium powder, 4 DEG C save backup；
2, by 2 parts of sodium carboxymethylcelluloses, 2 parts of starch, 6 parts of diatomite, 8 parts of flyash, 10 parts of expanded perlites and 32
Part water is mixed and stirred for uniformly, obtaining absorption mixture, this absorption mixture is aseptically placed 4d；
3,0.8 part of beauveria bassiana, 0.8 part of Paecilomyces lilacinus, 0.8 part of Trichoderma harzianum are added in 40 parts of absorption mixtures
Opportunistic pathogen powder, obtain absorption microorganism mixture；
4, by 4 parts of acrylamides, 8 parts of xylitols, 12 parts of clays, 20 parts of straw powders, 4 parts of plant growth regulating substances, 4 parts
Composite synergist is mixed evenly, and obtains Synergistic blend；
5,1:1 in mass ratio is uniformly mixed the mixture and Synergistic blend of absorption microorganism, obtains compounding microorganism
Pesticide.
After adopting the above structure, present embodiment has the beneficial effect that a kind of wide spectrum described in present embodiment
Microbial pesticide and preparation method thereof will be mainly used for preventing and treating beauveria bassiana, the prevention and treatment of subterranean pest-insect (grub, cutworm etc.)
The Paecilomyces lilacinus of root-knot nematode and prevention and treatment soil-borne disease fungal pathogens trichoderma harzianum compounding mixing, to the harmful organism in soil into
Row integrated control, the straw powder containing larger proportion in pesticide, while can also increase the content of organic matter of soil, it plays certain
Improve the effect of the soil texture；Have the function of wide spectrum prevention with prevention and treatment diseases and pests of agronomic crop, to grub, cutworm, garlic maggot,
The control efficiency that the harmful organisms such as bradysia odoriphaga, root-knot nematode, tomato late blight, capsicum late blight, cucumber fusarium axysporum have had, and produce
Quality is stable, efficient, nontoxic, safe to the human body, pollution-free, preserves the ecological environment, and energy substituting chemical pesticide is raw for agricultural
It produces, adapts to the demand for development of " green agriculture ".
Embodiment two:
Broad-spectrum micro-organisms pesticide in the present embodiment, it includes following parts by weight ingredient: 1.0 parts of beauveria bassiana, pale purple
1.0 parts of Paecilomyces varioti, 1.0 parts of Trichoderma harzianum, 10.5 parts of expanded perlite, 2.1 parts of sodium carboxymethylcellulose, 2.1 parts of starch, diatom
6.3 parts of soil, partial size are 100 μm, and 8.4 parts of the flyash that porosity is 40%, grain diameter are 400 μm, carbohydrate content
For 50% 17.5 parts of straw powder, 3.5 parts of acrylamide, 7 parts of xylitol, 10.5 parts of clay, plant growth regulating substance 3.5
Part, 3.5 parts of composite synergist, 33.6 parts of water.
Further, the plant growth regulating substance be heteroauxin, 2,4-D, methyl α-naphthyl acetate, gibberellin mixing and
At.
Further, the composite synergist include amino acid, borax, zinc sulfate, ammonium nitrate, chitosan mixing and
At.
Further, the beauveria bassiana, Paecilomyces lilacinus, strain content is 80~10,000,000,000 in Trichoderma harzianum
A spore/mL.
The preparation method of broad-spectrum micro-organisms pesticide in the present embodiment, its step are as follows:
1, the preparation of Biocontrol microorganism opportunistic pathogen powder:
1.1, the preparation of beauveria bassiana: by the strain inoculated of the beauveria bassiana of preservation in PDA culture medium, 28 DEG C of perseverances
After temperature culture 2-4d, the culture dish that bacterial strain grows fine is chosen in sterile working, and taking diameter in colony edge is 5mm pure culture biscuits involvng inoculation
In the conical flask equipped with 50mL optimization Cha Shi liquid medium through sterilizing in advance, at 28 DEG C, 150r/min shaken cultivation
It after 7-8d, is transferred in solid fermentation culture by 10% inoculum concentration, mixes, be placed in constant temperature incubation in 28 DEG C of constant incubators
10d or so；Take out fermentation material, 35 DEG C of baking 36h in baking oven；Pulverizer crushes, and crosses 200 meshes to get opportunistic pathogen powder, 4 DEG C of preservations are arrived
It is spare；
1.2, by the strain inoculated of the Paecilomyces lilacinus of preservation in PDA culture medium, 28 the preparation of Paecilomyces lilacinus: are placed in
After cultivating 5-6d in DEG C incubator, choose the culture dish that bacterial strain grows fine, colony edge take diameter be 5mm pure culture biscuits involvng inoculation in
In PDB culture solution, 28 DEG C, 150r/min shaken cultivation 5d are transferred in solid fermentation culture by 10% inoculum concentration, are mixed,
It is placed in constant temperature incubation 10d or so in 28 DEG C of constant incubators；Take out fermentation material, 35 DEG C of baking 36h in baking oven；Pulverizer crushes, mistake
To get opportunistic pathogen powder is arrived, 4 DEG C save backup 200 meshes；
1.3, the preparation of Trichoderma harzianum: the trichoderma harzianum strain of preservation is seeded in PDA culture medium, 28 DEG C, culture 3-
After 4d, the culture dish that bacterial strain grows fine is chosen, taking diameter in colony edge is 5mm pure culture biscuits involvng inoculation in PD culture solution, 28 DEG C,
It after 150r/min shaken cultivation 5d, is transferred in solid fermentation culture by 10% inoculum concentration, mixes, be placed in 28 DEG C of constant temperature incubations
Constant temperature incubation 10d or so in case；Take out fermentation material, 35 DEG C of baking 36h in baking oven；Pulverizer crushes, and crosses 200 meshes to get original is arrived
Bacterium powder, 4 DEG C save backup；
2, by 2.1 parts of sodium carboxymethylcelluloses, 2.1 parts of starch, 6.3 parts of diatomite, 8.4 parts of flyash, 10.5 parts of expansions
Perlite and 33.6 parts of water are mixed and stirred for uniformly, obtaining absorption mixture, this absorption mixture is aseptically placed
4d；
3,1.0 parts of beauveria bassianas, 1.0 parts of Paecilomyces lilacinus, 1.0 parts of Trichoderma harzianums are added in 50 parts of absorption mixtures
Opportunistic pathogen powder, obtain absorption microorganism mixture；
4, by 3.5 parts of acrylamides, 7 parts of xylitols, 10.5 parts of clays, 17.5 parts of straw powders, 3.5 parts of plant growth regulatings
Substance, 3.5 parts of composite synergists are mixed evenly, and obtain Synergistic blend；
5,1:1 in mass ratio is uniformly mixed the mixture and Synergistic blend of absorption microorganism, obtains compounding microorganism
Pesticide.
The above is only used to illustrate the technical scheme of the present invention and not to limit it, and those of ordinary skill in the art are to this hair
The other modifications or equivalent replacement that bright technical solution is made, as long as it does not depart from the spirit and scope of the technical scheme of the present invention,
It is intended to be within the scope of the claims of the invention.
Claims (7)
1. a kind of broad-spectrum micro-organisms pesticide, it is characterised in that: it includes following parts by weight ingredient: 0.8-1.0 parts of beauveria bassiana,
0.6-1.0 parts of Paecilomyces lilacinus, 8-12 parts of expanded perlite, 1-3 parts of sodium carboxymethylcellulose, forms sediment at 0.8-1.3 parts of Trichoderma harzianum
2-4 parts of powder, 7-12 parts of flyash, 15-30 parts of straw powder, 3~5 parts of acrylamide, 5~8 parts of xylitol, glues 6-8 parts of diatomite
9-13 parts native, 3-5 parts of plant growth regulating substance, 3-6 parts of composite synergist, 27.9~38.5 parts of water.
2. a kind of broad-spectrum micro-organisms pesticide according to claim 1, it is characterised in that: the partial size of the flyash is 50
~200 μm, porosity is 60%~80%.
3. a kind of broad-spectrum micro-organisms pesticide according to claim 1, it is characterised in that: the grain diameter of the straw powder
It is 200~500 μm, carbohydrate content is 40%~50%.
4. a kind of broad-spectrum micro-organisms pesticide according to claim 1, it is characterised in that: the plant growth regulating substance
For one of heteroauxin, 2,4-D, methyl α-naphthyl acetate, gibberellin, brassin lactones, cycocel, sodium humate, paclobutrazol or
It is a variety of to mix.
5. a kind of broad-spectrum micro-organisms pesticide according to claim 1, it is characterised in that: the composite synergist includes ammonia
Base acid, borax, zinc sulfate, ammonium nitrate, chitosan, Tea Saponin, ferrous sulfate, copper sulphate, manganese sulfate, ammonium molybdate, in urea extremely
Few five kinds mix.
6. a kind of broad-spectrum micro-organisms pesticide according to claim 1, it is characterised in that: the beauveria bassiana, pale purple
Strain content is 80~10,000,000,000 spore/mL in Paecilomyces varioti, Trichoderma harzianum.
7. a kind of preparation method of broad-spectrum micro-organisms pesticide, it is characterised in that: its step are as follows:
(1), the preparation of Biocontrol microorganism opportunistic pathogen powder:
(1.1), the preparation of beauveria bassiana: by the strain inoculated of the beauveria bassiana of preservation in PDA culture medium, 28 DEG C of constant temperature
After cultivating 2-4d, the culture dish that grows fine of bacterial strain is chosen in sterile working, colony edge take diameter be 5mm pure culture biscuits involvng inoculation in
In the conical flask equipped with 50mL optimization Cha Shi liquid medium through sterilizing in advance, at 28 DEG C, 150r/min shaken cultivation 7-
It after 8d, is transferred in solid fermentation culture by 10% inoculum concentration, mixes, be placed in constant temperature incubation 10d in 28 DEG C of constant incubators
Left and right；Take out fermentation material, 35 DEG C of baking 36h in baking oven；Pulverizer crushes, and crosses 200 meshes to get opportunistic pathogen powder is arrived, 4 DEG C of preservations are standby
With；
(1.2), by the strain inoculated of the Paecilomyces lilacinus of preservation in PDA culture medium, 28 DEG C the preparation of Paecilomyces lilacinus: are placed in
After cultivating 5-6d in incubator, choose the culture dish that grows fine of bacterial strain, colony edge take diameter be 5mm pure culture biscuits involvng inoculation in
In PDB culture solution, 28 DEG C, 150r/min shaken cultivation 5d are transferred in solid fermentation culture by 10% inoculum concentration, are mixed,
It is placed in constant temperature incubation 10d or so in 28 DEG C of constant incubators；Take out fermentation material, 35 DEG C of baking 36h in baking oven；Pulverizer crushes, mistake
To get opportunistic pathogen powder is arrived, 4 DEG C save backup 200 meshes；
(1.3), the preparation of Trichoderma harzianum: the trichoderma harzianum strain of preservation is seeded in PDA culture medium, 28 DEG C, culture 3-4d
Afterwards, the culture dish that bacterial strain grows fine is chosen, taking diameter in colony edge is 5mm pure culture biscuits involvng inoculation in PD culture solution, 28 DEG C,
It after 150r/min shaken cultivation 5d, is transferred in solid fermentation culture by 10% inoculum concentration, mixes, be placed in 28 DEG C of constant temperature incubations
Constant temperature incubation 10d or so in case；Take out fermentation material, 35 DEG C of baking 36h in baking oven；Pulverizer crushes, and crosses 200 meshes to get original is arrived
Bacterium powder, 4 DEG C save backup；
(2), according to mass ratio 1:1:3:4:5:16 sodium carboxymethylcellulose, starch, diatomite, flyash, expanded perlite
It is mixed and stirred for uniformly, obtaining absorption mixture with water, this absorption mixture is aseptically placed into 4d；
(3), the opportunistic pathogen powder of beauveria bassiana, Paecilomyces lilacinus, Trichoderma harzianum is added in absorption mixture, adsorbs mixture:
Beauveria bassiana: Paecilomyces lilacinus: the ratio of Trichoderma harzianum is 50:1:1:1, obtains the mixture of absorption microorganism；
(4), according to mass ratio 1:2:3:5:1:1 acrylamide, xylitol, clay, straw powder, plant growth regulating substance,
Composite synergist is mixed evenly, and obtains Synergistic blend；
(5), 1:1 in mass ratio is uniformly mixed the mixture and Synergistic blend of absorption microorganism, obtains compounding microorganism agriculture
Medicine.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111202097A (en) * | 2020-01-14 | 2020-05-29 | 河南农贝得农业科技有限公司 | Agricultural microbial agent for preventing and treating nematode diseases and preparation method thereof |
| CN111848242A (en) * | 2020-07-29 | 2020-10-30 | 福建三炬生物科技股份有限公司 | Compound microbial agent with biocontrol function and preparation method and application thereof |
| CN113396936A (en) * | 2021-04-26 | 2021-09-17 | 海南金雨丰生物工程有限公司 | Disease-preventing and insect-preventing composition and preparation method and application thereof |
| CN113831182A (en) * | 2021-09-30 | 2021-12-24 | 海南金雨丰生物工程有限公司 | Trichoderma harzianum compound bacterial fertilizer and preparation method and application thereof |
| CN114223677A (en) * | 2022-02-09 | 2022-03-25 | 潍坊绿威特生物工程有限公司 | Microbial agent for preventing and treating ginger nematode and application thereof |
| CN114831132A (en) * | 2022-06-09 | 2022-08-02 | 遵义市农业科学研究院 | Pesticide for preventing and treating cayenne root-knot nematode and preparation method thereof |
| CN115093294A (en) * | 2022-06-16 | 2022-09-23 | 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) | Microbial pesticide preparation for plant protection and growth regulation |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111202097A (en) * | 2020-01-14 | 2020-05-29 | 河南农贝得农业科技有限公司 | Agricultural microbial agent for preventing and treating nematode diseases and preparation method thereof |
| CN111848242A (en) * | 2020-07-29 | 2020-10-30 | 福建三炬生物科技股份有限公司 | Compound microbial agent with biocontrol function and preparation method and application thereof |
| CN113396936A (en) * | 2021-04-26 | 2021-09-17 | 海南金雨丰生物工程有限公司 | Disease-preventing and insect-preventing composition and preparation method and application thereof |
| CN113831182A (en) * | 2021-09-30 | 2021-12-24 | 海南金雨丰生物工程有限公司 | Trichoderma harzianum compound bacterial fertilizer and preparation method and application thereof |
| CN114223677A (en) * | 2022-02-09 | 2022-03-25 | 潍坊绿威特生物工程有限公司 | Microbial agent for preventing and treating ginger nematode and application thereof |
| CN114831132A (en) * | 2022-06-09 | 2022-08-02 | 遵义市农业科学研究院 | Pesticide for preventing and treating cayenne root-knot nematode and preparation method thereof |
| CN115093294A (en) * | 2022-06-16 | 2022-09-23 | 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) | Microbial pesticide preparation for plant protection and growth regulation |
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Application publication date: 20190723 |