CN106490000A - A kind of agricultural insecticidal bactericidal composition - Google Patents

A kind of agricultural insecticidal bactericidal composition Download PDF

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Publication number
CN106490000A
CN106490000A CN201610916928.3A CN201610916928A CN106490000A CN 106490000 A CN106490000 A CN 106490000A CN 201610916928 A CN201610916928 A CN 201610916928A CN 106490000 A CN106490000 A CN 106490000A
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China
Prior art keywords
parts
beauveria bassiana
green meat
culture
seat bacterium
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CN201610916928.3A
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Chinese (zh)
Inventor
曾升华
李肖宇
石仕福
吴俊杰
梁小文
石峥
罗建辉
李英武
罗双燕
王根豪
严艺波
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Jiangxi Tianxiang General Aviation Co Ltd
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Jiangxi Tianxiang General Aviation Co Ltd
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Priority to CN201610916928.3A priority Critical patent/CN106490000A/en
Publication of CN106490000A publication Critical patent/CN106490000A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom

Abstract

The invention provides a kind of agricultural insecticidal bactericidal composition, including the component of following parts by weight:20~85 parts of medicine activity component, the medicine activity component are beauveria bassiana and into green meat seat bacterium;5~65 parts of pharmaceutical carrier;1.0~15 parts of auxiliary agent.In the present invention, the compounding of beauveria bassiana and into green meat seat bacterium, so as to get agricultural insecticidal bactericidal composition efficiently, low toxicity, insecticidal effect is stable, and to people and animals and crop safety, both with cover crop, and can not injure natural enemy, safe, good environmental protection.Test result indicate that, containing the agricultural insecticidal bactericidal composition compounded into green meat seat bacterium and beauveria bassiana, insecticidal effect is up to 85%, and bactericidal effect is up to 84%, and pesticide-germicide effect will be significantly larger than and individually spray beauveria bassiana or into green meat seat bacterium.

Description

A kind of agricultural insecticidal bactericidal composition
Technical field
A kind of the present invention relates to technical field of pesticide, more particularly to agricultural insecticidal bactericidal composition.
Background technology
China is large agricultural country, and for thousands of years always based on planting industry, agricultural is the source of the basic necessities of life of the mankind, survives it This, is that other departments are able to independent and development basis, is the basis of national economy, and therefore agriculture development will be directly affected The development of national economy.But in agricultural production, the generation of pest and disease damage often has a strong impact on crop growth, makes crops A series of change and destruction is there occurs from physiological function to organizational structure, and then causes a large amount of underproduction of crops, to grower Bring huge economic loss.Therefore to effective preventing and treating of crop pests be instant, with ensure grain security and The economic interests of grower.
The species of diseases and pests of agronomic crop has many kinds at present, and wherein topmost insect pest is lepidoptera pest and coleoptera evil Worm, such as Pieris rapae, diamondback moth, bollworm, Pyrausta nubilalis (Hubern)., chafer and subterranean pest-insect Holotrichia diomphalia Batess, Gryllotalpa.Disease mainly has stricture of vagina withered The diseases such as disease, rice blast, downy mildew, bakanae disease, water rice brown spot, rice green smut and droop.These disease pests Harmful generation, causes a large amount of underproduction of crops and causes the quality of crops to reduce.In prior art, disease-resistant variety is usually selected Or pest control with insecticide antibacterial carrys out prevention and elimination of disease and pests.When being prevented and treated using pest control with insecticide antibacterial, although prevention effect is preferable, It is that pest control with insecticide antibacterial has difficult degradation, is easily destroyed the balance of ecosystem, people and animals is endangered larger.
Content of the invention
In view of this, present invention aims to the deficiencies in the prior art, and a kind of agricultural insecticidal sterilization group is provided Compound, agricultural insecticidal bactericidal composition prepared by the present invention, may be up to more than 85% to the control rate of insect pest, the preventing and treating to disease Effect up to more than 84%, pesticide-germicide effect will significantly larger than be used alone beauveria bassiana or into green meat seat bacterium.
The invention provides a kind of agricultural insecticidal bactericidal composition, including the component of following parts by weight:
20~85 parts of medicine activity component, the medicine activity component are beauveria bassiana and into green meat seat bacterium;
5~65 parts of pharmaceutical carrier;
1.0~15 parts of auxiliary agent.
Preferably, including medicine activity component that parts by weight are 45~65.
Preferably, the beauveria bassiana is (1.5~10.2) with the mass ratio into green meat seat bacterium:(4.0~15).
Preferably, effective spore content of the beauveria bassiana is 100~30,000,000,000/gram.
Preferably, the effective spore amount into green meat seat bacterium is 50~15,000,000,000/gram.
Preferably, the beauveria bassiana is (4.5~8.5) with the mass ratio into green meat seat bacterium:(6.5~10).
Preferably, including pharmaceutical carrier that parts by weight are 20~50.
Preferably, the pharmaceutical carrier is included in kieselguhr, Pulvis Talci, coal dust, bentonite, Hydrocerussitum (Ceruse) and calcined kaolin One or more.
Preferably, including auxiliary agent that parts by weight are 5~10.
Preferably, it is characterised in that the auxiliary agent include alkylbenzenesulfonate formaldehyde condensation products, fat alcohol polyethylene ether, One or more in alkylnaphthalene sulfonate and wooden sodium.
The invention provides a kind of agricultural insecticidal bactericidal composition, including the component of following parts by weight:Pharmaceutically active group Divide 20~85 parts, the medicine activity component is beauveria bassiana and into green meat seat bacterium;5~65 parts of pharmaceutical carrier;Auxiliary agent 1.0~ 15 parts.In the present invention, the compounding of beauveria bassiana and into green meat seat bacterium, so as to get agricultural insecticidal bactericidal composition efficiently, Low toxicity, insecticidal effect are stable, and to people and animals and crop safety, both with cover crop, and can not injure natural enemy, safe, ring Guaranteed cost is good.Test result indicate that, containing the agricultural insecticidal bactericidal composition compounded into green meat seat bacterium and beauveria bassiana, parasite killing is imitated Fruit up to 85%, bactericidal effect is up to 84%, and pesticide-germicide effect will be significantly larger than and individually spray beauveria bassiana or into green meat Seat bacterium.
Biological deposits explanation
Into green meat seat bacterium (Hypocrea virens), Chinese microorganism strain preservation was deposited on 08 13rd, 2013 Administration committee's common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microorganism Institute, biological deposits numbering is CGMCC No.7995.
Beauveria bassiana (Beauveria bassiana), was deposited in Chinese microorganism strain guarantor on 08 13rd, 2013 Hide administration committee common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the micro- life of the Chinese Academy of Sciences Thing institute, biological deposits numbering is CGMCC NO.7997 or CGMCC NO.7998.
Specific embodiment
The invention provides a kind of agricultural insecticidal bactericidal composition, including the component of following parts by weight:
20~85 parts of medicine activity component, the medicine activity component are beauveria bassiana and into green meat seat bacterium;Medicine is carried 5~65 parts of body;1.0~15 parts of adjuvant.
The agricultural insecticidal bactericidal composition that the present invention is provided includes 20~85 parts of medicine activity components, preferably 45~65 Part, more preferably 55 parts.In the present invention, described medicine activity component is beauveria bassiana and into green meat seat bacterium, described Beauveria bassiana is preferably (1.5~10.2) with the mass ratio of into green meat seat bacterium:(4.0~15), more preferably (4.5~8.5): (6.5~10), most preferably (5.5~6.5):(7.5~8.5).
In beauveria bassiana of the present invention, spore content living is preferably 100~30,000,000,000/gram, more preferably 150~25,000,000,000/gram, most preferably 20,000,000,000/gram.
In the present invention, the beauveria bassiana be preferably selected from biological deposits numbering be CGMCC NO.7997 and/or The strain activation and culture of CGMCC NO.7998 is obtained.
In the present invention, the preparation method of described beauveria bassiana is comprised the following steps:
1) by beauveria bassiana actication of culture;
2) beauveria bassiana after the activation is inoculated in beauveria bassiana fluid medium carries out liquid culture, training Support 30~50h and stop culture, obtain beauveria bassiana culture fluid;
3) by the step 2) the beauveria bassiana culture fluid that obtains is inoculated in beauveria bassiana solid medium, carries out Solid fermentation, obtains beauveria bassiana.
In the present invention, the activation of beauveria bassiana strain is that the beauveria bassiana strain of preservation is inoculated in equipped with nutrition fine jade In the eggplant type bottle of fat culture medium, 25-28 DEG C of 8~12h of culture carries out activation culture.
In the present invention, the beauveria bassiana after the activation be inoculated in fluid medium inoculum concentration be preferably 20~ 30%, more preferably 23~27%, most preferably 25%.
In the present invention, the fluid medium preferably includes following components:30~50g/L of analysis for soybean powder, white sugar 20~ 40g/L, 0.01~0.05g/L of flour, 0.01~0.05g/L of dehydrated potato powder, dipotassium hydrogen phosphate 0.0002g/L;More preferably:Yellow 35~45g/L of Semen Glycines powder, 25~35g/L of white sugar, 0.02~0.04g/L of flour, 0.02~0.04g/L of dehydrated potato powder, phosphoric acid hydrogen Dipotassium 0.0002g/L;Most preferably:Analysis for soybean powder 40g/L, white sugar 30g/L, flour 0.03g/L, dehydrated potato powder 0.03g/L, phosphorus Sour hydrogen dipotassium 0.0002g/L.
In the present invention, the liquid culture is preferably used after 120~130 DEG C of 30~45min of sterilizing.The present invention is to training The equipment of foster base sterilizing does not have special restriction, using sterilizing installation well known to those skilled in the art, such as high steam Autoclave.
The present invention does not have special restriction to the culture device, using Zymolysis Equipment well known to those skilled in the art is Can, such as fermentation tank.
In the present invention, the temperature of the beauveria bassiana liquid culture is preferably 25~30 DEG C, and more preferably 26~29 DEG C, most preferably 28 DEG C;The pH value of the beauveria bassiana liquid culture is preferably 6.6~7.2, more preferably 6.8~7.0, Most preferably 6.9;Pressure inside the tank is preferably 0.05~0.1MPa, more preferably 0.07~0.08MPa;The liquid culture logical Tolerance is preferably 0.8~1.2V/V min, more preferably 1V/V min;The time of the liquid culture is preferably 30~50h, More preferably 35~45h, most preferably 40h.
After completing the beauveria bassiana liquid culture, the beauveria bassiana culture fluid for obtaining is inoculated in solid by the present invention In culture medium, solid fermentation is carried out, obtain solid beauveria bassiana.In the present invention, the beauveria bassiana fermentation liquid inoculation 20~30%, more preferably 23~27%, most preferably 25% is preferably in the inoculum concentration of solid medium.
In the present invention, the solid medium preferably includes the component of following weight portion:50~90 parts of Testa Tritici, Semen Maydis powder 10~30 parts, 5~15 parts of rice husk;More preferably 60~80 parts of Testa Tritici, 15~25 parts of Semen Maydis powder, 7~13 parts of rice husk;Most preferably 70 parts of Testa Tritici, 20 parts of Semen Maydis powder, 10 parts of rice husk.The present invention adds distilled water in the beauveria bassiana solid medium, makes Solid medium water content is preferably 45%~55%, more preferably 47%~52%, most preferably 50%, then carries out solid Fermentation.
In the present invention, the culture medium thickness of the solid culture are preferably 1~6cm, more preferably 2~4cm, most preferably For 3cm.
In the present invention, the temperature of the beauveria bassiana solid fermentation is preferably 24~30 DEG C, and more preferably 26~29 DEG C, most preferably 28 DEG C;The humidity of the beauveria bassiana solid fermentation preferably 40%~95%, more preferably 55%~ 75%, most preferably 65%;The time of the beauveria bassiana solid fermentation is preferably 48~96h, more preferably 60~84h, Most preferably 72h.
After the solid fermentation, preferred pair of the present invention obtains being collected for spore powder, obtains beauveria bassiana spore.? In the present invention, the collection of the spore powder is specifically carried out using the instrument of microorganism solid fermentation fungal spore separation equipment.Institute The instrument for stating microorganism solid fermentation fungal spore separation equipment is purchased from Jiangxi Tianren Ecology Co., Ltd..
Heretofore described effective ingredient includes into green meat seat bacterium, and described effective spore content in green meat seat bacterium is excellent Choosing for 50~15,000,000,000/gram, most preferably more preferably 75~12,500,000,000/gram, 10,000,000,000/gram.
In the present invention, described into green meat seat bacterium be preferably selected from biological deposits numbering be CGMCC NO.7995 strain Activation culture is obtained.
In the present invention, the preparation method into green meat seat bacterium preferably includes following steps:
1) will be into green meat seat bacterium actication of culture;
2) by the activation after be inoculated in in green meat seat bacterium level liquid culture medium into green meat seat bacterium and carry out one-level liquid Body culture, 35~50h of culture stop culture, obtain into green meat seat bacterium one-level culture fluid;
3) by the step 2) obtain be inoculated in into green meat seat bacterium secondary liquid culture into green meat seat bacterium one-level culture fluid Secondary liquid culture is carried out in base, and 50~75h of culture stops culture, obtains into green two grades of culture fluid of meat seat bacterium;
4) by the step 3) two grades of culture fluid obtaining are inoculated in in green meat seat bacterium solid medium, carry out solid and send out Ferment culture, obtains into green meat seat bacterium.
In the present invention, the activation into green meat seat bacterium strain is preferably and is inoculated in preservation into green meat seat bacterium strain In eggplant type bottle equipped with nutrient agar, 20~35 DEG C of 30~65h of culture carry out activation culture.
After the activation culture, the present invention by the activation after be inoculated in into green meat seat bacterium level liquid into green meat seat bacterium Level liquid culture is carried out in culture medium.In the present invention, the inoculum concentration of the one-level culture fluid preferably 10~30%, more excellent Elect 15~25%, most preferably 20% as.
In the present invention, the level liquid culture medium preferably includes the component of following masses percentage composition:Glucose 0.5%~2.0%, white sugar 0.5%~2.0%, analysis for soybean powder 0.1%~0.3%, ammonium sulfate 0.1%~0.3%, phosphoric acid hydrogen Dipotassium 0.05%~0.1%, potassium chloride 0.05%~0.1%, magnesium sulfate 0.01%~0.04%, iron sulfate 0.00%~ 0.004%, balance of water;More preferably glucose 0.8%~1.5%, white sugar 0.8%~1.5%, analysis for soybean powder 0.15%~ 0.25%th, ammonium sulfate 0.15%~0.25%, dipotassium hydrogen phosphate 0.06%~0.09%, potassium chloride 0.06%~0.09%, sulfur Sour magnesium 0.015%~0.03%, iron sulfate 0.001%~0.003%, balance of water;Most preferably glucose 1%, white sugar 1%th, analysis for soybean powder 0.2%, ammonium sulfate 0.2%, dipotassium hydrogen phosphate 0.08%, potassium chloride 0.08%, magnesium sulfate 0.02%, iron sulfate 0.002%, balance of water.The present invention is not particularly limited to the source of above-mentioned medicine, using well known to those skilled in the art The medicine for preparing culture medium.
In the present invention, the level liquid culture medium is preferably used after 120~130 DEG C of 30~45min of sterilizing.At this In invention, more preferably 125 DEG C of the sterilising temp;The sterilization time is more preferably 40min.The present invention is gone out to culture medium Bacterium equipment is not particularly limited, using sterilizing installation well known to those skilled in the art, such as high-pressure steam sterilizing pan.
In the present invention, the temperature of the level liquid culture is preferably 20~32 DEG C, more preferably 25~30 DEG C, most Preferably 28 DEG C;The time of the level liquid culture is preferably 35~50h, more preferably more preferably 40~48h, 45h.
The original ph into green meat seat bacterium level liquid culture be preferably 5.5~6.5, more preferably 5.8~ 6.2, most preferably 6.0;
The present invention is continually fed into filtrated air in the level liquid incubation, is entered in 24h after being preferably inoculated with, Ventilation is 5~25m3/ h, after culture 24h, ventilation is 20~30m3/h;Enter after being more preferably inoculated with 24h, ventilation For 10~20m3/ h, after culture 24h, ventilation is 22~28m3/h;Enter in 24h after being most preferably inoculated with, ventilation is 15m3/ h, after culture 24h, ventilation is 25m3/h.The present invention does not have special restriction to the equipment for being passed through filtrated air, Using aeration equipments well known to those skilled in the art, such as air compressor machine.
After obtaining into green meat seat bacterium level liquid culture fluid, the present invention will be obtained into green meat seat bacterium level liquid culture fluid It is inoculated in in green meat seat bacterium secondary liquid culture medium, carries out secondary liquid culture, obtains into green meat seat bacterium secondary liquid culture Liquid.In the present invention, the inoculum concentration of the secondary liquid culture is preferably 10~30%, more preferably 15~25%, most preferably For 20%.
In the present invention, the secondary liquid culture medium preferably includes the component of following mass content:Glucose 1%~ 3%th, white sugar 1%~3%, dehydrated potato powder 0.5%~1.5%, analysis for soybean powder 0.5%~1.5%, Semen Maydis powder 0.3%~ 1.0%th, dipotassium hydrogen phosphate 0.01%~0.02%, balance of water;More preferably glucose 1.5%~2.5%, white sugar 1.5%~2.5%, dehydrated potato powder 0.8%~1.2%, analysis for soybean powder 0.8%~1.2%, Semen Maydis powder 0.4%~0.7%, phosphoric acid Hydrogen dipotassium 0.012%~0.018%, balance of water;Most preferably glucose 2%, white sugar 2%, dehydrated potato powder 1%, Semen Glyciness Powder 1%, Semen Maydis powder 0.5%, dipotassium hydrogen phosphate 0.015%, balance of water.The present invention does not have special limit to the source of above-mentioned medicine System, using the medicine for preparing culture medium well-known to those skilled in the art.
In the present invention, the secondary liquid culture medium is preferably used after 120~130 DEG C of 30~45min of sterilizing.At this In invention, more preferably 125 DEG C of the sterilising temp;The sterilization time is more preferably 40min.The present invention is gone out to culture medium Bacterium equipment is not particularly limited, using sterilizing installation well known to those skilled in the art, such as high-pressure steam sterilizing pan.
The present invention does not have special restriction to the equipment of the fermentation, using Zymolysis Equipment well known to those skilled in the art , such as fermentation tank.
In the present invention, the temperature into green meat seat bacterium secondary liquid culture is preferably 25~35 DEG C, more preferably 28~32 DEG C, most preferably 30 DEG C;The time of the secondary liquid culture is preferably 50~75h, more preferably 60~70h, more Preferably 65h.
The original ph into green meat seat bacterium secondary liquid culture be preferably 5.5~6.5, more preferably 5.8~ 6.2, most preferably 6.0.
In the present invention, described preferably enter under the conditions of filtrated air is continually fed into into green meat seat bacterium secondary liquid culture OK, the program for being passed through filtrated air is particularly preferred as:It is inoculated within the 24h after secondary liquid culture medium, ventilation is 100~180m3/ h, more preferably 120~160m3/ h, most preferably 150m3/h;Culture 24h after, ventilation be 200~ 260m3/ h, more preferably 220~250m3/ h, most preferably 240m3/h.The present invention does not have to the equipment for being passed through filtrated air There is special restriction, using aeration equipments well known to those skilled in the art, such as air compressor machine.
After obtaining into green meat seat bacterium secondary liquid culture fluid, the present invention will be obtained into green meat seat bacterium secondary liquid culture fluid It is inoculated in in green meat seat bacterium solid medium, carries out solid fermentation, obtains into green meat seat bacterium.In the present invention, the solid The inoculum concentration of fermentation is preferably 10~30%, more preferably 15~25%, most preferably 20%.
In the present invention, the solid medium preferably includes the component of following weight portion:Testa Tritici 64%~76%, bean cake 8%~13%, Semen Maydis powder 7.6%~13.5%, rice husk 7.8%~13.4%, more preferably 68%~74%, bean cake 9%~ 11%th, Semen Maydis powder 8.4%~11.4%, rice husk 8.6%~11.2%;Most preferably Testa Tritici 70%, bean cake 10%, Semen Maydis powder 10%th, rice husk 10%.The present invention preferably adds distilled water described in green meat seat bacterium solid medium, contains solid medium The water yield is preferably 45%~55%, more preferably 47%~52%, most preferably 50%, then carries out solid fermentation.
The present invention is to the control of the ambient temperature of the solid culture process, bacterium material temperature degree and ambient humidity.In the present invention, The control program of the temperature and humidity is particularly preferred as:It is preferably in the inner control loop border humidity for entering 1~3d of solid culture 85~90%, more preferably 88%, ambient temperature is preferably 25~35 DEG C, and bacterium material temperature degree is controlled to 28~30 DEG C;Solid entering The inner control loop border humidity of body 4~5d of culture is 40~60%, and ambient temperature is 30 DEG C;Within 6~7d, ambient temperature is preferably 30~35 DEG C;Preferably turn over during the 32~36h of solid culture radiating;Preferably by nutritional solution during the 48~55h of solid culture It is sprayed onto on bacterium material.
In the present invention, it is that 2~7% glucoses and mass fraction are 0.0005 that the nutritional solution preferably includes mass concentration ~0.002% ZnCl2, more preferably 5% glucose and mass fraction is 0.001% ZnCl2.The present invention is to the Fructus Vitis viniferae Sugar and ZnCl2Source be not particularly limited, using conventional medication well known to those skilled in the art.
In the present invention, the sprinkling volume of the nutritional solution is preferably the 5~10%, more preferably 6~9 of bacterium material volume, Most preferably 7%.
In the present invention, the thickness of the solid medium is preferably 2~5cm, more preferably 2.5~3.5cm, most preferably For 3cm.
Complete described into after green meat seat bacteria solid fermentation, preferred pair of the present invention obtains being collected for spore powder, obtains into Green meat seat bacterium.In the present invention, the collection into green meat seat bacterium spore is specifically using microorganism solid fermentation fungal spore point Instrument from equipment is carried out.The instrument of the microorganism solid fermentation fungal spore separation equipment is purchased from Jiangxi day people ecology share Company limited.
In the present invention, the effective spore amount into green meat seat bacterium that the solid fermentation is obtained is 50~15,000,000,000/gram.
The agricultural insecticidal bactericidal composition that the present invention is provided preferably includes 5~65 parts of pharmaceutical carriers, and more preferably 20~50 Part, most preferably 35 parts.In the present invention, described pharmaceutical carrier is not particularly limited, using commercial goods.Specifically Preferable kieselguhr, Pulvis Talci, coal dust, bentonite, one or more in Hydrocerussitum (Ceruse) and calcined kaolin, more preferably swelling Soil and calcined kaolin.When the pharmaceutical carrier is specially above two material, the matter of the bentonite and calcined kaolin Amount is than being preferably (10~25):(8~20), more preferably (15~20):(10~15), most preferably 18~12.In the present invention In, the pharmaceutical carrier has good medicine stability, can also improve the drug effect of medicine.
The agricultural insecticidal bactericidal composition that the present invention is provided preferably includes 1.0~15 parts of auxiliary agents, more preferably 5~10 parts, Most preferably 8 parts.The present invention does not have special restriction to the auxiliary agent, using commercial goods.It can be specifically alkylbenzene One or more in sulfonate and formaldehyde condensation substance, fat alcohol polyethylene ether, alkylnaphthalene sulfonate and wooden sodium, more preferably fat Alcohol polyvinylether and alkylnaphthalene sulfonate.When the auxiliary agent be specially above two material when, the fat alcohol polyethylene ether with The mass ratio of alkylnaphthalene sulfonate is preferably (1~10):(2~12), more preferably (3~8):(5~10), most preferably 5:8. In the present invention, described auxiliary agent can make medicine character stable, improve drug effect.
The present invention does not have special restriction to the preparation method of described agricultural insecticidal bactericidal composition, using this area skill The preparation method of compositionss known to art personnel.Specifically can be by described medicine activity component, pharmaceutical carrier and auxiliary agent Prepare after mixing.
Agricultural insecticidal bactericidal composition of the present invention can be applicable to crops prevention and elimination of disease and pests, specifically, can apply Crop specie include cereal crops, industrial crops, vegetable crop, orchard crop, forage crop etc..Agriculture of the present invention All kinds of insect pests, such as Pieris rapae, diamondback moth, bollworm, Pyrausta nubilalis (Hubern)., chafer and underground can be prevented and treated with insecticidal and bactericidal composition Insect Holotrichia diomphalia Batess, Gryllotalpa, the disease that can be prevented and treated have banded sclerotial blight, rice blast, downy mildew, bakanae disease, water rice brown spot, water The disease such as rice false smut and droop.
The present invention is not particularly limited to the application method of described agricultural insecticidal bactericidal composition, using art technology Application method known to personnel.Following steps specifically may include:Agricultural insecticidal bactericidal composition of the present invention is used After water dilution obtains 1000~5000 times of diluents, foliage-spray is carried out.
Embodiment in below in conjunction with the present invention, to the present invention in technical scheme be clearly and completely described.Aobvious So, described embodiment is only a part of embodiment of the invention, rather than whole embodiment.Reality in based on the present invention Example is applied, the every other embodiment obtained under the premise of creative work is not made by those of ordinary skill in the art all belongs to In the scope of protection of the invention.
Following examples active constituent content is pressed after hundred kilograms of folding is calculated and is added, and wherein beauveria bassiana such as nothing is referred in particular to Go out spore content, spore content is 100~30,000,000,000/gram, into green meat seat bacterium as without spore content is particularly pointed out, spore content is 50~150 Hundred million/gram.
Embodiment 1
Preparation into green meat seat bacterium:Preservation is inoculated in the eggplant type equipped with nutrient agar into green meat seat bacterium strain In bottle, 30 DEG C of culture 35h carry out activation culture;121 DEG C are inoculated in into green meat seat bacterium with 20% inoculum concentration after activation culture In level liquid culture medium after sterilizing 35min, specific condition of culture is:Temperature is 28 DEG C;PH value is 6.0;Inoculation is laggard Enter in 24h, ventilation is 15m3/ h, after culture 24h, ventilation is 25m3/h;Stop culture after culture 45h, obtain into green meat seat Bacterium level liquid culture fluid;The one-level culture fluid for obtaining is inoculated in into green meat seat bacterium secondary liquid culture with 20% inoculum concentration In liquid, specific condition of culture is:Temperature is 30 DEG C;PH value is 6.0;Enter in 24h after inoculation, ventilation is 150m3/ h, training After foster 24h, ventilation is 240m3/h;Stop culture after culture 65h, obtain into green meat seat bacterium secondary liquid culture fluid;To obtain Be inoculated in solid medium into green two grades of culture fluid of meat seat bacterium with 20% inoculum concentration, in solid medium add distillation Water, it is 50% to make solid medium water content, then carries out solid fermentation culture, and fermentation culture conditions are:Entering solid training The inner control loop border humidity for supporting 1~3d is preferably 90%, and ambient temperature is preferably 25 DEG C, and bacterium material temperature degree is controlled to 30 DEG C;Entering The inner control loop border humidity for entering 4~5d of solid culture is 60%, and ambient temperature is 30 DEG C;Within 6~7d, ambient temperature is preferably 35℃;Preferably turn over during the solid culture 36h radiating, nutritional solution is sprayed onto on bacterium material in solid culture 50h;By solid Bacterium material after fermentation collects into green meat seat bacterium spore, after testing, Cheng Lv using microorganism solid fermentation fungal spore separation equipment Effective spore amount of meat seat bacterium is 50~15,000,000,000/gram.
Embodiment 2
The preparation of beauveria bassiana:The beauveria bassiana strain of preservation is inoculated in the eggplant type equipped with nutrient agar In bottle, 26 DEG C of culture 9h carry out activation culture;Beauveria bassiana after activation culture is inoculated in 121 DEG C with 25% inoculum concentration and goes out In fluid medium after bacterium 35min, liquid culture is carried out in fermentation tank, specific condition of culture is:Temperature is 28 DEG C;pH It is worth for 6.9;Pressure inside the tank is 0.08MPa, and culture ventilation is 1V/V min;Stop culture after culture 40h, obtain ball spore white Stiff bacteria culture fluid;The ball spore Bombyx Batryticatus culture fluid for obtaining is inoculated in solid medium with 25% inoculum concentration, in solid culture Distilled water is added in base, it is 50% to make solid medium water content, then carries out solid fermentation, and fermentation condition is:Fermentation temperature For 26 DEG C, fermentation humidity is 65%, and fermentation time is 72h;Bacterium material after by solid fermentation adopts microbe in solid state culture funguses Spore separation equipment collects beauveria bassiana spore, and the effective spore amount of the beauveria bassiana for obtaining is 100~30,000,000,000/gram.Will The spore of collection examines under a microscope spore shape, to be defined as beauveria bassiana spore.
Embodiment 3
The preparation of agricultural insecticidal bactericidal composition:In terms of parts by weight, by the solid prepared in embodiment 1 into green meat 23.6 parts of the beauveria bassiana prepared in 31.4 parts of bacterium of seat, embodiment 2,20 parts of bentonite, 10 parts of calcined kaolin, fat 5 parts of alcohol polyvinylether, 5 parts of mixing of alkylnaphthalene sulfonate, obtains agricultural insecticidal bactericidal composition.
Embodiment 4
The preparation of agricultural insecticidal bactericidal composition:In terms of parts by weight, by the solid prepared in embodiment 1 into green meat 12.3 parts of the beauveria bassiana prepared in 32.7 parts of bacterium of seat, embodiment 2,15 parts of bentonite, 15 parts of calcined kaolin, coal dust 10 parts, 5 parts of alkylbenzenesulfonate formaldehyde condensation products, the 10 parts of mixing of wooden sodium obtain agricultural insecticidal bactericidal composition.
Embodiment 5
The preparation of agricultural insecticidal bactericidal composition:In terms of parts by weight, by the solid prepared in embodiment 1 into green meat 21.7 parts of the beauveria bassiana prepared in 43.3 parts of bacterium of seat, embodiment 2,15 parts of Hydrocerussitum (Ceruse), 10 parts of kieselguhr, alkyl benzene sulphonate 5 parts of salt formaldehyde condensation products, 5 parts of mixing of fat alcohol polyethylene ether, obtain agricultural insecticidal bactericidal composition.
Embodiment 6
The preparation of agricultural insecticidal bactericidal composition:In terms of parts by weight, by the solid prepared in embodiment 1 into green meat 32.2 parts of the beauveria bassiana prepared in 37.8 parts of bacterium of seat, embodiment 2,10 parts of Hydrocerussitum (Ceruse), 5 parts of Pulvis Talci, 10 parts of kieselguhr, 5 parts of mixing of fat alcohol polyethylene ether, obtain agricultural insecticidal bactericidal composition.
Embodiment 7
Prevent containing the agricultural insecticidal bactericidal composition into green meat seat bacterium and beauveria bassiana to what embodiment 3~6 was obtained The test of insect pest is controlled, and the compositionss of beauveria bassiana are comprised only as control, the results are shown in Table 1.
The test of 1 agricultural insecticidal bactericidal composition insecticidal effect of table
As can be drawn from Table 1, the agricultural insecticidal bactericidal composition that prepared by the present invention is up to the prevention effect of insect pest More than 85%, its insecticidal effect significantly larger than individually sprays beauveria bassiana.
Prevent containing the agricultural insecticidal bactericidal composition into green meat seat bacterium and beauveria bassiana to what embodiment 3~6 was obtained The test of disease is controlled, and the compositionss of green meat seat bacterium are comprised only into as control, the results are shown in Table 2.
The test of 2 agricultural insecticidal bactericidal composition bactericidal effect of table
As can be drawn from Table 2, the agricultural insecticidal bactericidal composition that prepared by the present invention is up to the prevention effect of disease More than 84%, its bactericidal effect significantly larger than individually sprays into green meat seat bacterium.
As seen from the above embodiment, the agricultural insecticidal bactericidal composition that prepared by the present invention, may be up to the control rate of insect pest More than 85%, the prevention effect up to more than 84% to disease, pesticide-germicide effect will significantly larger than be used alone ball spore Bombyx Batryticatus Bacterium or into green meat seat bacterium.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should It is considered as protection scope of the present invention.

Claims (10)

1. a kind of agricultural insecticidal bactericidal composition, including the component of following parts by weight:
20~85 parts of medicine activity component, the medicine activity component are beauveria bassiana and into green meat seat bacterium;
5~65 parts of pharmaceutical carrier;
1.0~15 parts of auxiliary agent.
2. compositionss according to claim 1, it is characterised in that including the pharmaceutically active group that parts by weight are 45~65 Point.
3. compositionss according to claim 1, it is characterised in that the beauveria bassiana and the mass ratio into green meat seat bacterium For (1.5~10.2):(4.0~15).
4. compositionss according to claims 1 to 3 any one, it is characterised in that effective spore of the beauveria bassiana Sub- content is 100~30,000,000,000/gram.
5. compositionss according to claims 1 to 3 any one, it is characterised in that the effective spore into green meat seat bacterium Son amount is 50~15,000,000,000/gram.
6. compositionss according to claims 1 to 3 any one, it is characterised in that the beauveria bassiana with into green meat The mass ratio of seat bacterium is (4.5~8.5):(6.5~10).
7. compositionss according to claim 1, it is characterised in that including the pharmaceutical carrier that parts by weight are 20~50.
8. compositionss according to claim 1 or 7, it is characterised in that the pharmaceutical carrier include kieselguhr, Pulvis Talci, One or more in coal dust, bentonite, Hydrocerussitum (Ceruse) and calcined kaolin.
9. compositionss according to claim 1, it is characterised in that be 5~10 auxiliary agents including parts by weight.
10. compositionss according to claim 1 or 9, it is characterised in that the auxiliary agent includes that alkylbenzenesulfonate formaldehyde contracts One or more in compound, fat alcohol polyethylene ether, alkylnaphthalene sulfonate and wooden sodium.
CN201610916928.3A 2016-10-21 2016-10-21 A kind of agricultural insecticidal bactericidal composition Pending CN106490000A (en)

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CN104073439A (en) * 2013-12-06 2014-10-01 江西天人生态股份有限公司 Beauveria bassiana(Bals.-Criv.)Vuill and application thereof
CN104087510A (en) * 2013-12-06 2014-10-08 江西天人生态股份有限公司 Beauveria bassiana and application thereof
CN104798821A (en) * 2015-04-24 2015-07-29 于书强 Double-effect microorganism microbial inoculum with sterilization and insecticide functions and preparation method of double-effect microorganism microbial inoculum

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CN1463604A (en) * 2002-06-03 2003-12-31 杨爱民 Pest control agent comprising multiple micro-organism
CN103756911A (en) * 2013-12-05 2014-04-30 江西天人生态股份有限公司 Hypocrea virens and its application
CN104073439A (en) * 2013-12-06 2014-10-01 江西天人生态股份有限公司 Beauveria bassiana(Bals.-Criv.)Vuill and application thereof
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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