CN108925362A - A kind of cultural method of high-yield disease resisting black fungus - Google Patents
A kind of cultural method of high-yield disease resisting black fungus Download PDFInfo
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- CN108925362A CN108925362A CN201810822569.4A CN201810822569A CN108925362A CN 108925362 A CN108925362 A CN 108925362A CN 201810822569 A CN201810822569 A CN 201810822569A CN 108925362 A CN108925362 A CN 108925362A
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- black fungus
- cultural method
- starch
- heap
- disease resisting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of cultural methods of high-yield disease resisting black fungus, the present invention by starch dispersion in water, gelatinization is mixed into starch solution with industrial formic acid and short-chain amylose activator, by tramp material material heap after discarded auricuralia auricular bran inoculating starter, sucrose, agar etc. and ironic citrate, sodium alginate are mixed with starch solution again, sprays and keeps spontaneous fermentation on fermentation heap, after sterilizing carries out inoculation black fungus after pack, after carrying out bacterium germination culture and daily management maturation, pick in time.The present invention keeps the black fungus bacterial piece coloring of cultivation good by preparing culture material using starch crosslinker solution and waste blackfungus mushroom bran, with sufficient sunlight, avoids the infringement of a part of miscellaneous bacteria, the quality of black fungus is improved, growth cycle is short, and yield is high, quality is good, and economic benefit is good.
Description
Technical field
The invention belongs to edible mushrooms to cultivate field, and in particular to a kind of cultural method of high-yield disease resisting black fungus.
Background technique
Black fungus is the edible fungus of Basidiomycetes Auriculariale Auriculariaceae, saprophytic on withered tree limb, Dan Shenghuo
All living creatures, shape is flat, and shaped like human ear, color is dark brown, and quality is transparent in colloidal substance, thin and flexible, delicious flavour, and nutrition is rich
Richness, can element can meat or fish, nutritive value is high, contains protein, fat, sugar, crude fibre, calcium, phosphorus, iron and much vitamins, quilt
Modern nutriology man highly praise is " meat or fish in element ".Black fungus also has very high medical value, its gas sweet in flavor is flat, have nourishing, beneficial stomach,
Promoting blood circulation is moisturized and other effects, blood lipid can be inhibited to rise, and prevents the fat deposition of the aortas such as cardiac muscle, liver, can be substantially reduced or be delayed
Atherogenesis.To hypertension, diarrhea, menorrhalgia, drops tears, mole sore, has a toothache and attached help curative effect.
Summary of the invention
The present invention provides a kind of cultural method of high-yield disease resisting black fungus, and the present invention is by utilizing starch crosslinker solution and giving up
It abandons auricuralia auricular bran and prepares culture material, keep the black fungus bacterial piece coloring of cultivation good with sufficient sunlight, avoid a part
The infringement of miscellaneous bacteria improves the quality of black fungus, and growth cycle is short, and yield is high, and quality is good, and economic benefit is good.
The present invention is achieved by the following technical solutions:
A kind of cultural method of high-yield disease resisting black fungus, which comprises the following steps:
(1) preparation of starch solution
By starch dispersion in 10-30 times of cold water, 60-90 DEG C is heated under agitation gelatinization 1-2 hours, be cooled to 20-35
DEG C, it is activated with the short-chain amylose of sulfuric acid tune pH to 2-3, the industrial formic acid and 0.08-0.4% that are slowly added to mixed liquor weight 40%
Agent is to slowly warm up to 40 DEG C and starts stirring, and time 30-60min is then cooled to room temperature, with ammonium hydroxide tune pH to 7.0 or so,
For use；
(2) preparation of culture base-material
A) suitable discarded auricuralia auricular bran is put into basin, 1-2% leavening is uniformly mixed in mushroom bran, is then uniformly spilt
Sawdust, the stalk material heap entered, and stir evenly；
B) by the sucrose of appropriate ratio, agar, yeast extract, magnesium sulfate, potassium sulfate, urea and microelement addition step in other
(1) abundant dissolution is boiled in the mixing of gained starch solution, heating, and then addition ironic citrate, sodium alginate stir evenly, after sterilizing
It is cooling；
C) spray gained mixed solution to the auricuralia auricular bran material heap after fermentation 3-5 days, adjusting moisture to 60-70% or so,
Heap growth 2-2.2m, high 0.8-1m, the flat-top trapezoidal heap of wide 1.2-1.5 m are taken the form supplemental oxygen of artificial turning, are started
Every turning in 3-7 days is primary, keeps spontaneous fermentation；
D) water content of the mushroom bran after spontaneous fermentation is controlled in 50-60%, is spread out, be aerated ventilation thickness, be packed into plastics bacterium
Kind bag, is punched in strain bag after the completion of filling, and opening is placed, for use；
(3) it is inoculated with
After carrying out inoculation black fungus by sterile working regulation using the resulting packed mushroom bran of step (2), it is transferred to culturing room and carries out bacterium
It the silk culture 2-3 month, is promoted and keeps normal temperature laboratory, dark, dry environment, mycelia carries out light-exposed culture when covering with bacterium bag, logical daily
Wind take a breath 1-2 time, it is 30-40 minute each, keep long-time illumination, it is daily carry out watering and pre- antiforeign bacteria, black fungus at
After ripe mark, picked in time.
The ratio of the ironic citrate is also to contain 0.5-2g ironic citrate in every 100g cultivation Bag Material.
The middle microelement includes zinc sulfate, ferrous sulfate, manganese sulfate, copper sulphate, boric acid and ammonium molybdate, dosage it
Than being 1: 2: 1: 2: 2: 1.
The sterilization treatment is that 100-120 DEG C of temperature, sterilizing in 3-4 hours is carried out to bacterium bag in pressure 0.1-0.15MPa
Processing.
The black fungus maturity symbol is that auricle length is opened, and edge is involute, and the outside of belly slightly has white spore.
The invention has the advantages that
In water by starch dispersion, gelatinization is mixed into starch solution with industrial formic acid and short-chain amylose activator to the present invention, will give up
Tramp material material heap after the auricuralia auricular bran inoculating starter of abandoning, then by sucrose, agar etc. and ironic citrate, sodium alginate and starch
Solution mixing, spray and keep spontaneous fermentation on fermentation heap, after pack sterilizing carry out inoculation black fungus after, carry out bacterium germination culture with
After daily management is mature, pick in time.Starch is as carrier, wherein containing saccharic, vitamin needed for edible fungi growth, mineral
The nutriments such as matter, industrial formic acid is crosslinked starch with short-chain amylose activator, sprays application in mushroom bran later, so that bacterium
Chaff has stickiness, while can adjust the slow release of fertilizer in mushroom bran, is fermented material using waste blackfungus mushroom bran, passes through system
Standby fertilizer starch solution adjusts C/N ratio and moisture, and inoculation fermentation microbial inoculum promotes its fermentation to improve the utilization rate of mushroom bran, opens
Mouth places the gas discharge generated during the fermentation for being, cultivates Bag Material sufficient raw, technology is simple, at low cost, can
Regional agriculture productivity effect, ecological benefits and peasant's increasing both production and income are improved, the sufficient sun is directly exposed to after black fungus bacterium germination
Can make the coloring of black fungus bacterial piece good under light, while avoid the infringement of a part of miscellaneous bacteria, and enhance its speed of growth and
Anti-hybrid ability improves the quality of black fungus, and growth cycle is short, and yield is high, and quality is good, and economic benefit is good.The present invention passes through
Using starch crosslinker solution and waste blackfungus mushroom bran culture material is prepared, the black fungus bacterial piece of cultivation is made with sufficient sunlight
Color is good, avoids the infringement of a part of miscellaneous bacteria, improves the quality of black fungus, and growth cycle is short, and yield is high, and quality is good, warp
Benefit of helping is good.
Specific embodiment
A kind of cultural method of high-yield disease resisting black fungus, which comprises the following steps:
(1) preparation of starch solution
By starch dispersion in 20 times of cold water, 70 DEG C are heated under agitation and is gelatinized 2 hours, 30 DEG C are cooled to, with sulfuric acid tune
PH to 2-3 is slowly added to the industrial formic acid of mixed liquor weight 40% and the short-chain amylose activator of 0.08-0.4%, is to slowly warm up to
40 DEG C are started stirring, and time 40min is then cooled to room temperature, with ammonium hydroxide tune pH to 7.0 or so, for use；
(2) preparation of culture base-material
A) suitable discarded auricuralia auricular bran is put into basin, 1-2% leavening is uniformly mixed in mushroom bran, is then uniformly spilt
Sawdust, the stalk material heap entered, and stir evenly；
B) by the sucrose of appropriate ratio, agar, yeast extract, magnesium sulfate, potassium sulfate, urea and microelement addition step in other
(1) abundant dissolution is boiled in the mixing of gained starch solution, heating, and then addition ironic citrate, sodium alginate stir evenly, after sterilizing
It is cooling；
C) gained mixed solution, adjusting moisture to 60-70% or so, heap are sprayed to the auricuralia auricular bran material heap after fermentation 3 days
Grow up 2-2.2m, high 0.8-1m, and the flat-top trapezoidal heap of wide 1.2-1.5 m takes the form supplemental oxygen of artificial turning, starts every
Turning in 3-7 days is primary, keeps spontaneous fermentation；
D) water content of the mushroom bran after spontaneous fermentation is controlled in 50-60%, is spread out, be aerated ventilation thickness, be packed into plastics bacterium
Kind bag, is punched in strain bag after the completion of filling, and opening is placed, for use；
(3) it is inoculated with
After carrying out inoculation black fungus by sterile working regulation using the resulting packed mushroom bran of step (2), it is transferred to culturing room and carries out bacterium
Silk culture 2 months, is promoted and keeps normal temperature laboratory, dark, dry environment, and mycelia carries out light-exposed culture when covering with bacterium bag, is divulged information daily
Ventilation 2 times 340 minutes every time, keeps long-time illumination, and daily to carry out watering and pre- antiforeign bacteria, black fungus has maturity symbol
Afterwards, it is picked in time.
The ratio of the ironic citrate is also to contain 1g ironic citrate in every 100g cultivation Bag Material.
The middle microelement includes zinc sulfate, ferrous sulfate, manganese sulfate, copper sulphate, boric acid and ammonium molybdate, dosage it
Than being 1: 2: 1: 2: 2: 1.
The sterilization treatment is that 110 DEG C of temperature, 3 hours sterilization treatments are carried out to bacterium bag in pressure 0.12MPa.
The black fungus maturity symbol is that auricle length is opened, and edge is involute, and the outside of belly slightly has white spore.
Claims (5)
1. a kind of cultural method of high-yield disease resisting black fungus, which comprises the following steps:
The preparation of starch solution
By starch dispersion in 10-30 times of cold water, 60-90 DEG C is heated under agitation gelatinization 1-2 hours, be cooled to 20-35
DEG C, it is activated with the short-chain amylose of sulfuric acid tune pH to 2-3, the industrial formic acid and 0.08-0.4% that are slowly added to mixed liquor weight 40%
Agent is to slowly warm up to 40 DEG C and starts stirring, and time 30-60min is then cooled to room temperature, with ammonium hydroxide tune pH to 7.0 or so,
For use；
(2) preparation of culture base-material
A) suitable discarded auricuralia auricular bran is put into basin, 1-2% leavening is uniformly mixed in mushroom bran, is then uniformly spilt
Sawdust, the stalk material heap entered, and stir evenly；
B) by the sucrose of appropriate ratio, agar, yeast extract, magnesium sulfate, potassium sulfate, urea and microelement addition step in other
(1) abundant dissolution is boiled in the mixing of gained starch solution, heating, and then addition ironic citrate, sodium alginate stir evenly, after sterilizing
It is cooling；
C) spray gained mixed solution to the auricuralia auricular bran material heap after fermentation 3-5 days, adjusting moisture to 60-70% or so,
Heap growth 2-2.2m, high 0.8-1m, the flat-top trapezoidal heap of wide 1.2-1.5 m are taken the form supplemental oxygen of artificial turning, are started
Every turning in 3-7 days is primary, keeps spontaneous fermentation；
D) water content of the mushroom bran after spontaneous fermentation is controlled in 50-60%, is spread out, be aerated ventilation thickness, be packed into plastics bacterium
Kind bag, is punched in strain bag after the completion of filling, and opening is placed, for use；
(3) it is inoculated with
After carrying out inoculation black fungus by sterile working regulation using the resulting packed mushroom bran of step (2), it is transferred to culturing room and carries out bacterium
It the silk culture 2-3 month, is promoted and keeps normal temperature laboratory, dark, dry environment, mycelia carries out light-exposed culture when covering with bacterium bag, logical daily
Wind take a breath 1-2 time, it is 30-40 minute each, keep long-time illumination, it is daily carry out watering and pre- antiforeign bacteria, black fungus at
After ripe mark, picked in time.
2. a kind of cultural method of high-yield disease resisting black fungus according to claim 1, which is characterized in that the citric acid
The ratio of iron is also to contain 0.5-2g ironic citrate in every 100g cultivation Bag Material.
3. a kind of cultural method of high-yield disease resisting black fungus according to claim 1, which is characterized in that micro member in described
Element includes zinc sulfate, ferrous sulfate, manganese sulfate, copper sulphate, boric acid and ammonium molybdate, and the ratio between dosage is 1: 2: 1: 2: 2: 1.
4. a kind of cultural method of high-yield disease resisting black fungus according to claim 1, which is characterized in that the sterilization treatment
For 100-120 DEG C of temperature, 3-4 hours sterilization treatments are carried out to bacterium bag in pressure 0.1-0.15MPa.
5. a kind of cultural method of high-yield disease resisting black fungus according to claim 1, which is characterized in that the black fungus
Maturity symbol is that auricle length is opened, and edge is involute, and the outside of belly slightly has white spore.
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| CN201810822569.4A CN108925362A (en) | 2018-07-25 | 2018-07-25 | A kind of cultural method of high-yield disease resisting black fungus |
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| CN201810822569.4A CN108925362A (en) | 2018-07-25 | 2018-07-25 | A kind of cultural method of high-yield disease resisting black fungus |
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| CN108925362A true CN108925362A (en) | 2018-12-04 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1463604A (en) * | 2002-06-03 | 2003-12-31 | 杨爱民 | Pest control agent comprising multiple micro-organism |
| CN102199050A (en) * | 2010-12-15 | 2011-09-28 | 沈阳科丰牧业科技有限公司 | Composite microbial fertilizer and preparation method thereof |
| CN103125270A (en) * | 2013-02-28 | 2013-06-05 | 深圳市仁泰生物科技有限公司 | High-yield antrodia cinnamomea mycelium fermentation method for triterpenoids |
| CN103650900A (en) * | 2012-08-31 | 2014-03-26 | 杨文博 | Method for cultivating black funguses |
| CN104845553A (en) * | 2015-06-04 | 2015-08-19 | 吴棋栋 | Adhesive and preparation method thereof |
| CN106588280A (en) * | 2016-11-24 | 2017-04-26 | 颍上县唐垛湖现代农业科技有限公司 | Method for culturing edible mushrooms with effective disease prevention |
-
2018
- 2018-07-25 CN CN201810822569.4A patent/CN108925362A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1463604A (en) * | 2002-06-03 | 2003-12-31 | 杨爱民 | Pest control agent comprising multiple micro-organism |
| CN102199050A (en) * | 2010-12-15 | 2011-09-28 | 沈阳科丰牧业科技有限公司 | Composite microbial fertilizer and preparation method thereof |
| CN103650900A (en) * | 2012-08-31 | 2014-03-26 | 杨文博 | Method for cultivating black funguses |
| CN103125270A (en) * | 2013-02-28 | 2013-06-05 | 深圳市仁泰生物科技有限公司 | High-yield antrodia cinnamomea mycelium fermentation method for triterpenoids |
| CN104845553A (en) * | 2015-06-04 | 2015-08-19 | 吴棋栋 | Adhesive and preparation method thereof |
| CN106588280A (en) * | 2016-11-24 | 2017-04-26 | 颍上县唐垛湖现代农业科技有限公司 | Method for culturing edible mushrooms with effective disease prevention |
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Application publication date: 20181204 |