Prior art
Insect pathogenic fungus is most important insecticidal microorganism monoid, and about under field conditions (factors) 70% insect disease is fungus-caused, and the insect pathogenic fungus of having found nearly more than 100 more than 700 of genus is planted.Disease fungus has safety, does not does not kill and wound advantage such as nontarget organism, is unique pathogenic microorganism with the characteristic of tagging simultaneously, its mechanism of causing a disease complexity, although thereby it be the microorganism that is applied to control of insect the earliest, find to have the resistant insect generation yet.Green muscardine fungus (Metarhizium) is the insect pathogenic fungus that is used for pest control the earliest, utilizes it to come the research of pest control and practice more century-old.According to incompletely statistics, existing nearly over one hundred kind of crops, forest and sanitary insect pest carry out the evaluation of laboratory experiment with green muscardine fungus, 150 kinds of insects are arranged approximately to carry out small-scale field controling test.Along with Brazil and Australia are extensive use of Metarhizium anisopliae control sugarcane and the herbage insect is obtained bigger progress, and the success of the test that prevents and treats locust in Africa with yellowish green green muscardine fungus, the research of green muscardine fungus has been entered climax.Over nearly 10 years, both at home and abroad the development of green muscardine fungus insecticide is obviously quickened, domestic and international registered product has more than 10.But there is the slow or more high shortcoming of cost of preventive effect instability, desinsection speed in fungus insecticide at present, has limited its extensive use.
In order to improve fungus insecticide desinsection speed, to reduce cost, fungus insecticide that people adopt usually and compatible chemical pesticide carry out composite, achieve the above object.Since Anderson and Roberts have selected 6 kinds of chemical pesticides such as sevin, furans pellet, gusathion m, methyl gusathion m, diflubenzuron and 5a,6,9,9a-hexahydro-6,9-methano-2,4 as composite dose when the research white muscardine fungi is mixed, the number of chemical agricultural chemicals is used in the middle of beauvericin insecticide composite, and has obtained good insecticidal effect.At present, the problem of three aspects of the mixed existence of chemical insecticide and fungus insecticide: the higher pollution that causes environment of content of chemical pesticide in the higher or complex preparation of (1) chemical pesticide toxicity; (2) chemical pesticide and fungal farm chemicals compatibility are relatively poor, influence the fungi bin stability; (3) fungal farm chemicals and chemical pesticide complex process are immature, as add the emulsifier influence fungi activity, solubilizer etc. in complex preparation.Up to now, domestic do not have a kind of disinsection fungal and chemical pesticide complex preparation to register.
Pyrethrin pesticide is the insect never poison of a kind of efficient, wide spectrum, low toxicity, low-residual, and tool stomach toxicity and action of contace poison because action site is single, many farmings, woods, is herded harmful insect pyrethrin pesticide is developed immunity to drugs; Simultaneously, the pyrethrin pesticide of high dose has certain toxicity to honeybee, fish and natural enemy insect, influence ecological environment, and these deficiencies make some pyrethrin pesticides be accepted degree to descend gradually that people have to seek new agricultural chemicals and substitute pyrethrin pesticide.As: sumicidin has been inhibited on the tealeaves and has used.Solve drug-fast problem as not adopting an effective measure and reduce field consumption, the destiny that multiple pyrethrin pesticide will inevitably be eliminated.
Adopt the pyrethrin pesticide of low dosage or sublethal dose to mix with green muscardine fungus, can bring into play fungus insecticide and pyrethrin pesticide advantage separately, accelerate the desinsection speed of fungal farm chemicals, can reduce the chemical pesticide consumption again significantly, reduce pollution environment, postponing insect produces the chemical pesticide pesticide resistance, therefore, has vast potential for future development.
Summary of the invention
It is good that one of purpose of the present invention provides a kind of compatibility, and can reduce the green muscardine fungus and the pyrethrin pesticide compounded pesticides of pyrethrin pesticide environmental pollution significantly.
Two of purpose of the present invention provides the preparation method of a kind of green muscardine fungus and pyrethrin pesticide compounded pesticides.
Compounded pesticides of the present invention mainly is made up of insect pathogenic fungus and pyrethrin pesticide, and the percentage by weight of insect pathogenic fungus and pyrethrin pesticide is: insect pathogenic fungus accounts for 90.0-99.0%, and pyrethrin pesticide accounts for 1.0-10.0%; Described insect pathogenic fungus is a green muscardine fungus, and described pyrethrin pesticide is the former medicine of natural pyrethrin or the former medicine of sumicidin or former medicine of Cyhalothrin or the former medicine of beta-cypermethrin.
Compounded pesticides main component weight optimal proportion of the present invention is: green muscardine fungus accounts for 90.0-93.0%, and natural pyrethrin accounts for 7-10%.
Compounded pesticides main component weight optimal proportion of the present invention is: green muscardine fungus accounts for 96.0-98.0%, and sumicidin accounts for 2-4%.
Compounded pesticides main component weight optimal proportion of the present invention is: green muscardine fungus accounts for 97.0-98.0%, and Cyhalothrin accounts for 2-3%.
Compounded pesticides main component weight optimal proportion of the present invention is: green muscardine fungus accounts for 94.0-96.0%, and beta-cypermethrin accounts for 4-6%.
Compounded pesticides of the present invention is applicable to the conidium that green muscardine fungus obtains by solid fermentation.Its weight is spore content (hundred million spores/g) of the pure conidial powder of conidium sum ÷.
In the compounded pesticides proportioning of the present invention, pyrethrin pesticide is by the conversion of effective ingredient net content.
The formulation of green muscardine fungus of the present invention and pyrethrin pesticide compounded pesticides is oil-suspending agent or pulvis.
The preparation method of compounded pesticides oil-suspending agent of the present invention is: the ratio requirement by the agriculture chemical compounding insecticide takes by weighing pure conidial powder of green muscardine fungus and the former medicine of pyrethrin pesticide respectively.Earlier pyrethrin pesticide is dissolved in the vegetable oil that weight content is the 0.1-1.0% antioxidant, add pure conidial powder of green muscardine fungus and pyrethrin pesticide weight 0.5-3 synergist doubly again, stirred 1 hour with 100-200 rev/min speed, add at last that to satisfy compounded pesticides be the vegetable oil of 0.1-1.0% antioxidant to the weight content of green muscardine fungus content requirement.
The preparation method of compounded pesticides pulvis of the present invention is: the green muscardine fungus culture and the medium of oven dry are pulverized, cross 300 orders and make the high cryptogam of green muscardine fungus, spore content with the high cryptogam of blood counting chamber detection green muscardine fungus converses the weight that is equivalent to the pure cryptogam of green muscardine fungus; Take by weighing pyrethrin pesticide and pyrethrin pesticide weight 0.5-3 synergist (as: Butacide) doubly by ratio requirement then, and pyrethrin pesticide and synergist be dissolved in the vegetable oil that weight content is the 0.1-1.0% antioxidant, adopt super-thin spray technology or the technology of mixing to contain the vegetable oil of pyrethrin pesticide again and the high cryptogam of green muscardine fungus of corresponding proportion mixes, add at last and satisfy the hydrogen matter calcium carbonate of compounded pesticides, mix getting final product in 30-40 minute with mixer with 100-200 rev/min speed the muscardine spore content requirement.
Above-mentioned antioxidant is butyl alkyl anisole or dibutyl hydroxy toluene or does not have sub-gallate-based propyl ester or dilauryl thiodipropionate.Described vegetable oil is an edible vegetable oil.As soybean oil, rapeseed oil, cotton seed oil, salad wet goods.
Compounded pesticides of the present invention has the following advantages:
1, pyrethrin pesticide and muscardine spore compatibility are good, and synergistic effect is obvious, compare with each single agent agricultural chemicals of its composition, have improved insecticidal toxicity.
2, the pyrethrin pesticide field dosage in the complex preparation is the 2-10% of pyrethrin pesticide list agent consumption, reduces its pollution to environment, to natural enemies security.
3, reduce production costs.The field consumption of green muscardine fungus is the 30-75% of the single agent of green muscardine fungus in the complex preparation, reduces the green muscardine fungus production cost.
4, can reduce the pyrethrin pesticide environmental pollution significantly, delay pest resistance to insecticide and produce.
5, production technology is simple.
Experimental data one, disease fungus spore and beta-cypermethrin compatible good
Take by weighing pyrethrin pesticide natural pyrethrin, sumicidin, Cyhalothrin, each 2g of the former medicine of beta-cypermethrin, the weight content that is dissolved in 89g respectively is in the vegetable oil of 1-10% vaseline and 0.1-1.0% antioxidant, add then pure conidial powder of 19g green muscardine fungus and 1g Butacide mixing evenly after, respectively make the 100g oil-suspending agent, it is in 26 ± 1 ℃ the environment, to be contrast not add pyrethrin pesticide that sealing is stored in temperature.Take out the oil-suspending agent 1g behind the mixing every month at random, on the 1/4SDA flat board, survey germination rate with glassine paper disk method.Cultivate down for 26 ± 1 ℃ and got the glassine paper disk in 24 hours with cotton blue dyeing back microscopy, count 200-300 spore, the spore count that record is sprouted calculates germination rate, and each handles three repetitions.
The compatibility of table 1 muscardine spore and pyrethrin pesticide
As can be seen from Table 1: pyrethrin pesticide natural pyrethrin, sumicidin, Cyhalothrin, beta-cypermethrin are that the back sealing that is mixed of 10: 90 ratio and green muscardine fungus xerospore is stored by weight, not significantly influence of germination rate to fungal spore illustrates the compatible good of natural pyrethrin, sumicidin, Cyhalothrin, beta-cypermethrin and muscardine spore.
The synergistic effect of experimental data two, green muscardine fungus and pyrethrin pesticide complex preparation
To kill locust green muscardine fungus CQMa102 is test strain, in above-mentioned compound proportion scope, dispose 10,000,000,000/ml green muscardine fungus natural pyrethrin oil-suspending agent, 10,000,000,000/ml green muscardine fungus sumicidin oil-suspending agent, 10,000,000,000/ml green muscardine fungus Cyhalothrin oil-suspending agent, 10,000,000,000/ml green muscardine fungus beta-cypermethrin oil-suspending agent and 100,000,000/g green muscardine fungus beta-cypermethrin pulvis.Measure green muscardine fungus, pyrethrin pesticide and complex preparation virulence respectively to the close healthy Asiatic migratory locust adult of 2-4 age in days body weight.Examination worm after the processing is put into rearing-box, raises with fresh wheat seeding, places 27 ± 1 ℃, and humidity 75-85% raises under the photoperiod 16h L/8hD condition, observes its borer population anyway in 4 days behind the confession medicine, calculates the lethality (%) under the various experimental concentration.With the DPS software statistics lethal dose of 50 (LD
50).Calculate co-toxicity coefficient (CTC) according to Sun method (1960), judge the co-toxicity effect of the high chlorine green muscardine fungus of 10,000,000,000 spores/mL with the CTC value.CTC value about 100 is a summation action, is the notable synergistic effect greater than 120 o'clock.To be defined as the standard agent with green muscardine fungus.Its toxicity index (TI) is 100.
Complex preparation is the virulence multiple=fungal farm chemicals list agent LD of fungal farm chemicals list agent
50/ complex preparation LD
50
Be blank with not administration simultaneously in the test, if lethality≤5% of blank locust o'clock, the lethality of administration group is not proofreaied and correct, during as if control group lethality 5-15%, selects by following formula the lethality of administration group is proofreaied and correct.
Table 2 pyrethrin pesticide and green muscardine fungus are to the co-toxicity effect of locust
Chrysanthemum ester class composition | Formulation | Weight proportion | LD50 (ug/ head) (95% confidence limit) | CTC |
Natural pyrethrin | Oil-suspending agent | 92∶8 | 0.29(0.15-0.62) | 413.30 |
Sumicidin | Oil-suspending agent | 97∶3 | 0.51(0.13-0.68) | 396.96 |
Cyhalothrin | Oil-suspending agent | 98∶2 | 0.28(0.12-0.55) | 381.81 |
Beta-cypermethrin | Oil-suspending agent | 96∶4 | 0.16(0.10-0.49) | 346.70 |
Beta-cypermethrin | Pulvis | 95∶5 | 0.21(0.13-0.52) | 329.15 |
Contrast (green muscardine fungus) | | - | 5.61(3.89-9.32) | - |
As can be seen from Table 2: in above-mentioned composite scope ratio, the co-toxicity coefficient of pyrethrin pesticide and green muscardine fungus (CTC) all greater than 120, illustrates that pyrethrin pesticide and green muscardine fungus complex preparation have the notable synergistic effect between 300-420.The purer green muscardine fungus preparation of complex preparation virulence improves 10-35 doubly.
Experimental data three, compounded pesticides Toxicity Determination
To kill locust green muscardine fungus CQMa102 is test strain, and preparation is 10,000,000,000/ml green muscardine fungus natural pyrethrin oil-suspending agent (92: 8), 2. 10,000,000,000/ml green muscardine fungus beta-cypermethrin oil-suspending agent (95: 5), 3. 100,000,000/g green muscardine fungus Cyhalothrin pulvis (98: 2), 4. 500,000,000/g green muscardine fungus sumicidin pulvis (97: 3), 5. 10,000,000,000/ml green muscardine fungus oil-suspending agent and 6. 100,000,000/g green muscardine fungus pulvis 1..In the first generation locust population of indoor feeding, select locust polypide heavy phase with healthy adult, put into that kind the diameter of wheat seeding to be arranged be 35cm * 50cm cylinder mould, 50 of every processing.The consumption ultra low volume spraying that oil-suspending agent is pressed the 50ul/ cage after diluting 5 times, pulvis dusts by the consumption of 1.5g/ cage.Be contrast if kill locust green muscardine fungus finish with not dispenser.Repeat 3 times.Regularly observe every day, changes fresh wheat seeding after three days.Adjusting insectary room temperature to 28 ± 1 ℃, relative moisture 60-85%, light dark period are 16h/8h.Observed once in 24 hours.In one week of continuous observation, each dead borer population of handling of record touches polypide with tweezers, and foot and worm wing are motionless to be dead.Data statistics is with reference to experimental data one
Table 3 pyrethrin pesticide and green muscardine fungus to the Toxicity Determination of locust (corrected mortality, %)
Medicament | 1d behind the medicine | 2d behind the medicine | 3d behind the medicine | 4d behind the medicine | 5d behind the medicine | 6d behind the medicine | LT
50(d)
|
① | 1.24 | 5.33 | 21.67 | 48.67 | 90.67 | 95.33 | 3.69 |
② | 3.67 | 11.67 | 23.00 | 59.67 | 94.33 | 97.67 | 3.31 |
③ | 5.33 | 13.67 | 19.67 | 63.67 | 93.33 | 98.67 | 3.24 |
④ | 3.33 | 10.67 | 20.33 | 54.67 | 95.33 | 100 | 3.38 |
⑤ | 0 | 2.67 | 7.33 | 12.33 | 25.33 | 34.67 | - |
⑥ | 0 | 1.33 | 5.33 | 8.67 | 21.67 | 29.33 | - |
As can be seen from Table 3: in above-mentioned composite scope ratio, pyrethrin pesticide and green muscardine fungus complex preparation are to the indoor virulence of the locust virulence apparently higher than the single agent of green muscardine fungus, and the desinsection time obviously shortens.The LT of each complex preparation
50At 3-4 days, behind the medicine 5 days corrected mortality greater than 90%, and the single agent of green muscardine fungus corrected mortality<30% of 5 days behind medicine.
Experimental data four, compounded pesticides field efficacy
To kill locust green muscardine fungus CQMa102 is test strain, prepares the high fluorocarbon oil suspending agent of 10,000,000,000/ml green muscardine fungus (green muscardine fungus: high chlorine weight ratio is 96: 4).Ultra low volume spraying.Data statistics is with reference to experimental data one.
The high fluorocarbon oil suspending agent control of table 4 10,000,000,000/ml green muscardine fungus Tibet migratory locusts field control effectiveness test
Handle | Extension rate | Dosage (ml/ hectare) | Insect population radix (head/m
2)
| Behind the medicine 1 day | Behind the medicine 3 days | Behind the medicine 5 days |
Residual worm amount (head/m
2)
| Preventive effect (%) | Residual worm amount (head/m
2)
| Preventive effect (%) | Residual worm amount (head/m
2)
| Preventive effect (%) |
Complex preparation | 7 | 1000 | 128.14 | 117.13 | 13.61 | 79.34 | 44.31 | 45.54 | 68.46 |
5 | 1000 | 151.71 | 129.60 | 13.75 | 98.19 | 41.79 | 15.21 | 91.10 |
3 | 1000 | 162.36 | 87.21 | 43.68 | 49.94 | 68.95 | 12.23 | 93.32 |
The single agent of green muscardine fungus | 3 | 1000 | 153.09 | 146.11 | 4.30 | 113.61 | 33.26 | 95.47 | 44.80 |
Blank | | | 134.41 | 142.25 | | 149.17 | | 151.11 | |
As can be seen from Table 4: the different extension rates of the high fluorocarbon oil suspending agent of 10,000,000,000/ml green muscardine fungus to the virulence of Tibet migratory locusts all apparently higher than the single agent of green muscardine fungus, the preventive effect of preventive effect of wherein diluting behind the complex preparation medicine of 3 times and 5 times 5 days is all greater than 90%, no evident difference, select for use during use the dilution 5 times after dispenser, can reach and significantly improve virulence, can reduce the consumption of green muscardine fungus and beta-cypermethrin again.
Specific embodiment
Embodiment 1:
Take by weighing former medicine of 1Kg beta-cypermethrin (weight effective content 97%) or the former medicine of 0.5Kg sumicidin (weight effective content 92%) or the former medicine of 2.3Kg natural pyrethrin (weight effective content 70%); Add and to be equipped with in 150 liters of blenders of soybean oil that the 5Kg weight content is 0.2% butyl alkyl anisole; Adding the 70Kg weight content after the dissolving again is the soybean oil of the fragrant ether of 0.1% butyl alkyl merchant, pure cryptogam of green muscardine fungus and the 1.25Kg Butacide that the 19Kg spore content is 50,000,000,000/g, stirs 0.5 hour with 100-200 rev/min speed; Be the fragrant ether vegetable oil dilution of 0.1% butyl alkyl merchant with weight content at last, making the content of the fungal spore in the oil-suspending agent is 10,000,000,000 spores/mL, makes 10,000,000,000/ml green muscardine fungus natural pyrethrin oil-suspending agent or 10,000,000,000/ml green muscardine fungus sumicidin oil-suspending agent or 10,000,000,000/ml green muscardine fungus beta-cypermethrin oil-suspending agent.
Embodiment 2:
Take by weighing the former medicine of 2.3Kg natural pyrethrin (weight effective content 70%); Add mixing in 150 liters of blenders of rapeseed oil that the 10Kg weight content is 0.5% dibutyl hydroxy toluene is housed; Add the rapeseed oil that the 70Kg weight content is 0.5% dibutyl hydroxy toluene, pure cryptogam of green muscardine fungus and the 2.3Kg Butacide that the 19Kg spore content is 1,000 hundred million/g again, stirred 0.5 hour with 100-200 rev/min speed; Be the rapeseed oil dilution of 0.1% dibutyl hydroxy toluene with weight content at last, make 20,000,000,000/ml green muscardine fungus natural pyrethrin oil-suspending agent.
Embodiment 3:
Take by weighing former medicine of 0.6Kg beta-cypermethrin (weight effective content 97%) or the former medicine of 0.3Kg Cyhalothrin (weight effective content 94%) back respectively and add that weight content is housed is 0.4% not have in 10 liters of blenders of cotton seed oil of sub-gallate-based propyl ester, add the 0.5Kg Butacide again, making gross weight is 4Kg, with 100-200 rev/min speed stir 0.5 hour standby; The green muscardine fungus medium of oven dry is pulverized, crossed 300 orders and make the high cryptogam of green muscardine fungus, the spore content that detects the high cryptogam of green muscardine fungus with blood counting chamber is that (it is 50,000,000,000/g) that the pure cryptogam of green muscardine fungus CQMa102 gets spore content to 11,000,000,000/g; The high cryptogam of 46Kg green muscardine fungus that will contain the pure cryptogam of the 10Kg spray tower of packing into, spray into standby cotton seed oil with high-pressure sprayer, the hydrogen matter calcium carbonate that adds 4950Kg again, with mixer mixing 30-40 minute 100,000,000/g green muscardine fungus beta-cypermethrin pulvis or 100,000,000/g green muscardine fungus Cyhalothrin pulvis.
Embodiment 4:
Taking by weighing former medicine of 0.7Kg beta-cypermethrin (weight effective content 97%) or the former medicine of 0.5Kg sumicidin (weight effective content 94%) back respectively adds and to be equipped with in 10 liters of blenders of soybean salad oil that weight content is 0.3% dilauryl thiodipropionate, add the 1Kg Butacide again, making gross weight is 10Kg, with 100-200 rev/min speed stir 0.5 hour standby; The green muscardine fungus culture of oven dry is connected the medium pulverizing, cross 300 orders and make the high cryptogam of green muscardine fungus, the spore content that detects the high cryptogam of green muscardine fungus with blood counting chamber is that (it is 1,000 hundred million/g) that the pure cryptogam of green muscardine fungus CQMa105 gets spore content to 12,000,000,000/g; The high cryptogam of 100Kg green muscardine fungus that will contain the pure cryptogam of the 12Kg spray tower of packing into, spray into standby soybean salad oil with high-pressure sprayer, the hydrogen matter calcium carbonate that adds 4890Kg again, with mixer mixing 30-40 minute 200,000,000/g green muscardine fungus beta-cypermethrin pulvis or 200,000,000/g green muscardine fungus sumicidin pulvis.