CN1500392A - Microorganism anti-disease anti-insect formulation and its making method - Google Patents
Microorganism anti-disease anti-insect formulation and its making method Download PDFInfo
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- CN1500392A CN1500392A CNA021452512A CN02145251A CN1500392A CN 1500392 A CN1500392 A CN 1500392A CN A021452512 A CNA021452512 A CN A021452512A CN 02145251 A CN02145251 A CN 02145251A CN 1500392 A CN1500392 A CN 1500392A
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- diseases prevention
- proof agent
- garlic
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Abstract
The microbial disease and pest preventing agent is compound spawn liquid produced through compound culture with Bacillus subtilis, globisporal muscardine, plant lactobacillus, swamp rhodopseudomonad and streptomycete. The production process of the microbial disease and pest preventing agent includes first alone culture of globisporal muscardine, the subsequent culture of the other spawns and fermentation together with garlic and other bactericidal matter. The microbial disease and pest preventing agent has the functions of strengthening plant metabolism to reduce disease, raising the nutrient absorbing capacity of plant root, inhibiting the growth of harmful bacteria, improving soil, killing and inhibiting pathogenic microbe in soil and preventing diseases and pests.
Description
Technical field:
The present invention relates to a kind of microorganism diseases prevention insect-proof agent and preparation method, can be used for preventing the generation of damage by disease and insect in the proportion of crop planting, belong to biological pesticide technical field.
Background technology:
At present, disclosed patent, publication number is CN92112764, wherein disclosed high-efficiency crop disease prevention yield increasing fungus TC26 is a bacillus subtilis, its preparation technology comprises bacillus subtilis separation screening, protospecies breeding, solid fermentation and liquid fermentation, this diseases prevention insect-proof agent only adopts a kind of spawn culture, and control efficiency is limited.
Summary of the invention:
Purpose of the present invention is exactly a kind of microorganism diseases prevention insect-proof agent and preparation method that adopts multiple bacterial classification produced with combination of invention.
For realizing above purpose, technical scheme of the present invention provides a kind of microorganism diseases prevention insect-proof agent, and its characteristics are, by composite bacteria stoste and be furnished with medium, garlic, capsicum, molasses, Chinese herbal medicine, it is formulated by following weight percentages:
Composite bacteria stoste 20-60%
Garlic 1-15%
Capsicum 1-15%
Molasses 20-60%
Chinese herbal medicine 1-15%
The optimum weight proportioning of each raw material of preparation of the present invention is:
Composite bacteria stoste 45%
Garlic 6%
Capsicum 2%
Molasses 45%
Chinese herbal medicine 2%
Composite bacteria stoste of the present invention is one of bacillus subtilis, beauveria bassiana, Lactobacillus plantarum, Rhodopseudomonas palustris, streptomycete or their mixture; Described Chinese herbal medicine is one of Radix Glycyrrhizae, honeysuckle, corter pseudolaricis or their mixture.
Preparation method of the present invention is characterized in that, after earlier beauveria bassiana being cultivated separately, carries out compound cultivation with other bacterial classifications again, and it comprises the following steps:
(1) from the root soil of multiple kinds of crops, screening and optimizing bacterial classification the environment such as in stubble, the pest body in spite of illness;
(2) earlier beauveria bassiana is cultivated separately in medium, cultivation temperature is 25-35 ℃, makes the conidium of beauveria bassiana reach 2-5 hundred million/gram;
(3) be to cultivate in one of molasses, peptone, dusty yeast, ammonium sulfate, magnesium sulfate or their mixture with other bacterial classifications at same medium again, the ph value is adjusted to 6.8-7.2; Cultivation temperature is that 25-35 ℃, time are 48-96 hour, obtains composite bacteria agent capable stoste;
(4) garlic, capsicum, Chinese herbal medicine are pulverized;
(5) composite bacteria agent capable stoste is added inserted in the vapor tight tank fermentation after garlic, capsicum, molasses, Chinese herbal medicine evenly mix 7 days, temperature is 30-35 ℃, obtains the kind of the foliage-spray and the pouring water reclamation of microorganism diseases prevention insect-proof agent;
(6) microorganism diseases prevention insect-proof agent is mixed corn flour, blue or green chaff, straw powder, mixed ratio is 1 to 5, after uniformly mixing, 25-36 ℃ of bottom fermentation 7 days, obtains the microorganism diseases prevention insect-proof agent of solid.
Microorganism diseases prevention insect-proof agent adopts the fermentation of growing jointly of antibacterial materials such as multiple bacterial classification and garlic, produces the beauveria bassiana element of big content simultaneously, and viable bacteria content liquid is 1,200,000,000/milliliter, and solid is 10,000,000,000/gram.
Beauveria bassiana can produce the beauveria bassiana element, the defense response that can suppress the insect haemocyte can be bred it in a large number in host, destroy host's function of organization, the a large amount of accumulation of its metabolite oxalate in blood can make host's metabolic disturbance and death simultaneously.The coenzyme that produces in the Rhodopseudomonas palustris metabolic process and discharge is a kind of disease-resistant factor, pathogenic bacteria in body and the soil are had certain inhibitory action, also can with the actinomycetes symbiosis in the soil, make profitable strain propagation, suppress other pernicious bacteria breedings, play ecological disease-proof functions.
Complex microbial community can produce and accumulate a large amount of nutritious microbial cells and useful metabolite in plant root and soil, as organic acid, alcohol, aldehyde, vitamin, hormone, antibiotic, can reach the purpose that comprehensively prevents damage by disease and insect.
Advantage of the present invention is:
1. can strengthen the metabolism of plant, strengthen the cuticula of blade protection film, prevent that pathogen from entering, the plant roots lignin is increased, disease is alleviated;
2. the ester composition that contains in the microorganism diseases prevention insect-proof agent does not decompose in herbvore pest body, can make insect produce physiological disorder and death;
3. microorganism fungus kind can also form collaborative mutually mycoderm in plant corpus or root, blade face definite value, can improve the nutrient absorption ability of plant root, effectively suppresses growth and the breeding of harmful bacterium.
Embodiment:
1. the preparation method of microorganism diseases prevention insect-proof agent:
(1) from the root soil of multiple kinds of crops, screening and optimizing bacterial classification the environment such as in stubble, the pest body in spite of illness;
(2) earlier beauveria bassiana is cultivated separately in medium, cultivation temperature is 25-35 ℃, makes ball reach 2-5 hundred million/gram from the conidium of stiff bacterium;
(3) the screening bacterial classification takes by weighing by following weight proportion;
Bacillus subtilis 25%, beauveria bassiana 30%, Lactobacillus plantarum 15%, Rhodopseudomonas palustris 18%, streptomycete 12%.
Take by weighing medium by following weight proportion:
Molasses 2.5%, peptone 0.6%, dusty yeast 0.2%, ammonium sulfate 0.3%, magnesium sulfate 0.2%, ph value 6.8.
All bacterial classifications are cultivated in same medium, and cultivation temperature is that 30 ℃, time are 72 hours, obtains composite bacteria agent capable stoste;
(4) garlic, capsicum, Chinese herbal medicine are pulverized;
(5) take by weighing composite bacteria agent capable and raw material by following weight proportion:
Composite bacteria stoste 45%, garlic 6%, capsicum 2%, molasses 45%, Chinese herbal medicine are chosen Radix Glycyrrhizae 2%.
Composite bacteria agent capable stoste added inserted in the vapor tight tank fermentation after garlic, capsicum, molasses, Chinese herbal medicine evenly mix 7 days, temperature is 30 ℃, obtains the kind of the foliage-spray and the pouring water reclamation of microorganism diseases prevention insect-proof agent.
2. the microorganism diseases prevention insect-proof agent preparation method of composite curing:
(6) microorganism diseases prevention insect-proof agent is mixed corn flour, blue or green chaff, straw powder, mixed ratio is 1 to 5, after uniformly mixing, 30 ℃ of temperature bottom fermentations 7 days, obtains the microorganism diseases prevention insect-proof agent of solid.
Pressing different proportion according to different usages uses:
Foliage-spray: dilution 200-300 doubly sprays with vaporific later, and the blade face tow sides all will be sprayed onto.
The pouring water reclamation: dilution 1000-1500 doubly waters later.
Soil melioration: when the crop basal dressing, evenly admix use, every mu of general 2-3kg that uses; When crop growth period uses, should after loosening the soil, apply, every mu of 5-6kg waters after the use.
Claims (5)
1. a microorganism diseases prevention insect-proof agent is characterized in that, by composite bacteria stoste and be furnished with medium, garlic, capsicum, molasses, Chinese herbal medicine, it is formulated by following weight percentages;
Composite bacteria stoste 20-60%
Garlic 1-15%
Capsicum 1-15%
Molasses 20-60%
Chinese herbal medicine 1-15%
2. a microorganism diseases prevention insect-proof agent is characterized in that, wherein the optimum weight proportioning of each raw material is:
Composite bacteria stoste 45%
Garlic 6%
Capsicum 2%
Molasses 45%
Chinese herbal medicine 2%
3. microorganism diseases prevention insect-proof agent according to claim 1, its characteristics are, described composite bacteria stoste is one of bacillus subtilis, beauveria bassiana, Lactobacillus plantarum, Rhodopseudomonas palustris, streptomycete or their mixture, and bacterial classification is from the root soil of multiple kinds of crops, screening and optimizing the environment such as in stubble, the pest body in spite of illness.
4. microorganism diseases prevention insect-proof agent according to claim 1, its characteristics are that described Chinese herbal medicine is one of Radix Glycyrrhizae, honeysuckle, corter pseudolaricis or their mixture.
5. a microorganism diseases prevention insect-proof agent preparation method is characterized in that, after earlier beauveria bassiana being cultivated separately, carries out compound cultivation with other bacterial classifications again, and it comprises the following steps:
(1) from the root soil of multiple kinds of crops, screening and optimizing bacterial classification the environment such as in stubble, the pest body in spite of illness;
(2) earlier beauveria bassiana is cultivated separately in medium, cultivation temperature is 25-35 ℃, makes the conidium of beauveria bassiana reach 2-5 hundred million/gram;
(3) be to cultivate in one of molasses, peptone, dusty yeast, ammonium sulfate, magnesium sulfate or their mixture with other bacterial classifications at same medium again, the ph value is adjusted to 6.8-7.2; Cultivation temperature is that 25-35 ℃, time are 48-96 hour, obtains composite bacteria agent capable stoste;
(4) garlic, capsicum, Chinese herbal medicine are pulverized;
(5) composite bacteria agent capable stoste is added inserted in the vapor tight tank fermentation after garlic, capsicum, molasses, Chinese herbal medicine evenly mix 6-8 days, temperature is 30-35 ℃, obtains the kind of the foliage-spray and the pouring water reclamation of microorganism diseases prevention insect-proof agent;
(6) microorganism diseases prevention insect-proof agent is mixed corn flour, blue or green chaff, straw powder, mixed ratio is 1 to 5, after uniformly mixing, 25-36 ℃ of temperature bottom fermentation 7 days, obtains the microorganism diseases prevention insect-proof agent of solid.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA021452512A CN1500392A (en) | 2002-11-12 | 2002-11-12 | Microorganism anti-disease anti-insect formulation and its making method |
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| CNA021452512A CN1500392A (en) | 2002-11-12 | 2002-11-12 | Microorganism anti-disease anti-insect formulation and its making method |
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Cited By (15)
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|---|---|---|---|---|
| CN102310966A (en) * | 2010-07-01 | 2012-01-11 | 郑州大学 | Storage mildew-proof and moth-proof packaging hemp cloth and preparation method thereof |
| CN101671212B (en) * | 2009-09-28 | 2013-06-12 | 上海创博现代自然农业（集团）有限公司 | Microbial agent capable of reducing leaf vegetable plant diseases and insect pests and preparing method thereof |
| CN103333813A (en) * | 2012-10-23 | 2013-10-02 | 辛玉江 | Compound microbial preparation and preparation method thereof |
| WO2014111764A1 (en) * | 2013-01-21 | 2014-07-24 | Federacion Nacional De Cafeteros De Colombia | Combination of biological pesticides |
| CN103992968A (en) * | 2014-04-22 | 2014-08-20 | 安徽清明环保科技有限公司 | Household garbage deodorizing and sterilizing composite inoculant and preparation method thereof |
| CN104824072A (en) * | 2015-05-20 | 2015-08-12 | 句容市茅山盛开园林有限公司 | Ornamental tree biopesticide and application method thereof |
| CN106305833A (en) * | 2016-08-22 | 2017-01-11 | 铜仁市万山区海洋农业科技发展有限公司 | Preparation for preventing anthracnose of rhizoma paris, and preparation method thereof |
| CN106332925A (en) * | 2016-08-22 | 2017-01-18 | 铜仁市万山区海洋农业科技发展有限公司 | Composition for controlling anthracnose of platycodon grandiflorum |
| CN107232242A (en) * | 2017-05-10 | 2017-10-10 | 颐中（青岛）实业有限公司 | A kind of preparation method for being used to extend the probiotics of flower ornamental phase |
| CN107410385A (en) * | 2017-04-21 | 2017-12-01 | 王现明 | A kind of method for producing insecticide for watermelon |
| CN110194991A (en) * | 2019-03-06 | 2019-09-03 | 上海市南洋模范中学 | Extracting method, equipment and the application of Bergamot |
| CN110771630A (en) * | 2019-10-15 | 2020-02-11 | 汤怀信 | Insecticide |
| CN112806397A (en) * | 2021-01-15 | 2021-05-18 | 东北农业大学 | Biopesticide for preventing and treating bacterial diseases of potatoes and preparation method thereof |
| CN114271295A (en) * | 2022-01-05 | 2022-04-05 | 上海中农智丰生物化学有限公司 | Microbial pesticide for killing insects and bacteria and preparation method thereof |
| CN114532163A (en) * | 2022-02-23 | 2022-05-27 | 王晓玉 | Agricultural seedling raising plug culture method |
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2002
- 2002-11-12 CN CNA021452512A patent/CN1500392A/en active Pending
Cited By (20)
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| CN101671212B (en) * | 2009-09-28 | 2013-06-12 | 上海创博现代自然农业（集团）有限公司 | Microbial agent capable of reducing leaf vegetable plant diseases and insect pests and preparing method thereof |
| CN102310966A (en) * | 2010-07-01 | 2012-01-11 | 郑州大学 | Storage mildew-proof and moth-proof packaging hemp cloth and preparation method thereof |
| CN103333813A (en) * | 2012-10-23 | 2013-10-02 | 辛玉江 | Compound microbial preparation and preparation method thereof |
| CN103333813B (en) * | 2012-10-23 | 2015-05-20 | 辛玉江 | Compound microbial preparation and preparation method thereof |
| US20150359229A1 (en) * | 2013-01-21 | 2015-12-17 | Federacion Nacional De Cafeteros De Colombia | Combination of biological pesticides |
| WO2014111764A1 (en) * | 2013-01-21 | 2014-07-24 | Federacion Nacional De Cafeteros De Colombia | Combination of biological pesticides |
| US10104896B2 (en) * | 2013-01-21 | 2018-10-23 | Federacion Nacional De Cafeteros De Colombia | Combination of biological pesticides |
| CN103992968B (en) * | 2014-04-22 | 2017-03-22 | 安徽全民环保科技有限公司 | Preparation method of household garbage deodorizing and sterilizing composite inoculant |
| CN103992968A (en) * | 2014-04-22 | 2014-08-20 | 安徽清明环保科技有限公司 | Household garbage deodorizing and sterilizing composite inoculant and preparation method thereof |
| CN104824072A (en) * | 2015-05-20 | 2015-08-12 | 句容市茅山盛开园林有限公司 | Ornamental tree biopesticide and application method thereof |
| CN106305833A (en) * | 2016-08-22 | 2017-01-11 | 铜仁市万山区海洋农业科技发展有限公司 | Preparation for preventing anthracnose of rhizoma paris, and preparation method thereof |
| CN106332925A (en) * | 2016-08-22 | 2017-01-18 | 铜仁市万山区海洋农业科技发展有限公司 | Composition for controlling anthracnose of platycodon grandiflorum |
| CN107410385A (en) * | 2017-04-21 | 2017-12-01 | 王现明 | A kind of method for producing insecticide for watermelon |
| CN107232242A (en) * | 2017-05-10 | 2017-10-10 | 颐中（青岛）实业有限公司 | A kind of preparation method for being used to extend the probiotics of flower ornamental phase |
| CN107232242B (en) * | 2017-05-10 | 2020-04-03 | 颐中（青岛）实业有限公司 | Preparation method of microecological preparation for prolonging ornamental period of flowers |
| CN110194991A (en) * | 2019-03-06 | 2019-09-03 | 上海市南洋模范中学 | Extracting method, equipment and the application of Bergamot |
| CN110771630A (en) * | 2019-10-15 | 2020-02-11 | 汤怀信 | Insecticide |
| CN112806397A (en) * | 2021-01-15 | 2021-05-18 | 东北农业大学 | Biopesticide for preventing and treating bacterial diseases of potatoes and preparation method thereof |
| CN114271295A (en) * | 2022-01-05 | 2022-04-05 | 上海中农智丰生物化学有限公司 | Microbial pesticide for killing insects and bacteria and preparation method thereof |
| CN114532163A (en) * | 2022-02-23 | 2022-05-27 | 王晓玉 | Agricultural seedling raising plug culture method |
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