CN107232242B - Preparation method of microecological preparation for prolonging ornamental period of flowers - Google Patents
Preparation method of microecological preparation for prolonging ornamental period of flowers Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
A preparation method of a microecological preparation for prolonging the ornamental period of flowers comprises the following steps: preparing Chinese herbal medicine extract and liquid culture medium, mixing the Chinese herbal medicine extract and the liquid culture medium, culturing bacillus and lactobacillus, inoculating and fermenting, separating and taking precipitate, preparing suspension, adding other components, and drying to obtain the finished product. The components of the Chinese herbal medicine extracting solution can inhibit the propagation of pathogenic bacteria and pests in soil, reduce the plant diseases and insect pests, promote the growth of flowers, improve the chlorophyll content of leaves, enhance the photosynthesis of plants, promote the synthesis of substances such as anthocyanin and the like, and prolong the ornamental period of the flowers; the vegetable powder is rich in nutrient substances such as starch, polysaccharide, protein and the like, can provide necessary nutrient substances for the growth of plants, and promotes the growth and development of the plants; after fermentation, many macromolecules are changed into small molecules, which is more beneficial to the absorption of plants.
Description
The technical field is as follows:
the invention relates to a preparation method of a microecological preparation for prolonging the ornamental period of flowers.
Background art:
the flowers with lasting faint scent can cultivate mood and beautify environment, and are more and more popular with consumers. Meanwhile, China is also a flower planting and exporting country, a large amount of flowers are sold in the international market every year, and the ornamental period of the flowers is shortened due to improper treatment and difference of environmental conditions. Therefore, people have attracted attention to prolong the ornamental period of flowers and improve the quality of flower products. At present, methods for prolonging the ornamental period of flowers at home and abroad mainly comprise physical methods and chemical medicament methods, and although the methods can prolong the ornamental period of the flowers, the methods also have some defects. The physical method mainly prolongs the ornamental period by changing the growth environmental conditions of flowers, such as: the temperature is reduced, the illumination condition is reduced, and the like, but the requirement of objective conditions required by a physical method is high, the physical method is not easy to control, the flexibility is poor, and the quality of a treated product is still difficult to ensure. The chemical agent method is mainly used for prolonging the ornamental period by spraying a chemical preservative, and the existing chemical agent method needs to use a large amount of pesticide agents, causes pesticide residues, pollutes the environment, is not easy to treat, and directly or indirectly influences the physical and mental health of people. The prior art has no solution to this problem.
The invention content is as follows:
the invention aims to overcome the defects in the prior art, and provides a preparation method of a microecological preparation for prolonging the ornamental period of flowers, which can inhibit the propagation of pathogenic bacteria and pests in soil, reduce plant diseases and insect pests, promote the growth of the flowers, improve the chlorophyll content of leaves, enhance the photosynthesis of the plants, promote the synthesis of substances such as anthocyanin and the like, prolong the ornamental period of the flowers and solve the problems in the prior art.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a preparation method of a microecological preparation for prolonging the ornamental period of flowers comprises the following steps:
s1, preparing Chinese herbal medicine extracting solution and liquid culture medium: the Chinese herbal medicine extract is prepared by grinding one or more of herba plantaginis, flos Lonicerae, herba Portulacae, Glycyrrhrizae radix, fructus Lycii, radix Angelicae sinensis, and fructus crataegi into powder, adding water with the above medicinal powder, boiling with slow fire, filtering, and collecting filtrate to obtain Chinese herbal medicine extract; the liquid culture medium is composed of the following raw materials in parts by weight: molasses: 3-7g, soybean meal: 20-30g, corn steep liquor: 40-60g, beef extract: 0.5-1.5g, sodium acetate: 3.0-8.0g, triammonium citrate: 1.0-3.0g, dipotassium hydrogen phosphate: 1.0-3.0g, magnesium sulfate: 0.1-0.5g, manganese sulfate: 0.02-0.18g, tween-80: 0.5-1.5 parts of distilled water: 700 portions and 1300 portions;
s2, preparing the Chinese herbal medicine extracting solution and a liquid culture medium according to the weight ratio of 20-30: 800-;
s3, respectively activating the bacillus and the lactic acid bacteria at 25-37 ℃ for 1-2min, inoculating the bacillus and the lactic acid bacteria into the culture medium obtained in S2 according to the proportion of 2-4%, and culturing at 25-37 ℃ at 30-100r/min until the logarithmic phase;
s4, inoculating the logarithmic phase zymocyte liquid into the residual culture medium prepared in the S2 according to the proportion of 1-5%, and culturing at 25-37 ℃ for 4-8d at 100-140 r/min;
s5, when the pH value of the fermentation liquor reaches 6.2-7.2 and the effective viable count reaches 2-10X 108CFU/mL, stopping fermentation, carrying out centrifugal separation, taking the precipitate and removing the supernatant;
s6, adding the precipitate into the Chinese herbal medicine extract with the same amount to obtain a suspension;
s7, mixing the following substances in parts by weight: 30-50 parts of suspension, 5-15 parts of trehalose and 800 parts of distilled water, and then filtering and sterilizing to obtain a mixed solution;
and S8, taking the fruit and vegetable straw powder subjected to autoclaving, mixing the mixed liquid obtained in the step S7 with 30-90 parts by weight of the fruit and vegetable straw powder, and drying to obtain a finished product.
Preferably, the bacillus is one or more of bacillus subtilis, bacillus coagulans and bacillus pumilus.
Preferably, the lactobacillus is one or more of lactobacillus casei, lactobacillus acidophilus, lactobacillus delbrueckii and lactobacillus brevis.
Preferably, the fruit and vegetable straw powder is prepared by grinding one or more of peanut shells, potatoes, tomatoes, eggplants, soybeans and the like into powder.
Preferably, freeze-drying is used in S8.
Preferably, in S5, the centrifugal separation conditions are: the rotating speed is 6000r/min, and the time is 5-8 min.
Compared with the prior art, the invention has the advantages that: the components of the Chinese herbal medicine extracting solution can inhibit the propagation of pathogenic bacteria and pests in soil, reduce the plant diseases and insect pests, promote the growth of flowers, improve the chlorophyll content of leaves, enhance the photosynthesis of plants, promote the synthesis of substances such as anthocyanin and the like, and prolong the ornamental period of the flowers; the vegetable powder is rich in nutrient substances such as starch, polysaccharide, protein and the like, can provide necessary nutrient substances for the growth of plants, and promotes the growth and development of the plants; after fermentation, many macromolecules are changed into small molecules, which is more beneficial to the absorption of plants.
The specific implementation mode is as follows:
in order to clearly illustrate the technical features of the present invention, the present invention is explained in detail by the following embodiments.
S1, preparing Chinese herbal medicine extracting solution and liquid culture medium: the Chinese herbal medicine extract is prepared by grinding one or more of herba plantaginis, flos Lonicerae, herba Portulacae, Glycyrrhrizae radix, fructus Lycii, radix Angelicae sinensis, and fructus crataegi into powder, adding water with the above medicinal powder, boiling with slow fire, filtering, and collecting filtrate to obtain Chinese herbal medicine extract; the liquid culture medium is composed of the following raw materials in parts by weight: molasses: 3-7 parts of soybean meal: 20-30 parts of corn steep liquor: 40-60 parts of beef extract: 0.5-1.5 parts, sodium acetate: 3.0-8.0 parts of triammonium citrate: 1.0-3.0 parts of dipotassium hydrogen phosphate: 1.0-3.0 parts, magnesium sulfate: 0.1-0.5 parts of manganese sulfate: 0.02-0.18 parts, tween-80: 0.5-1.5 parts of water: 700 portions and 1300 portions;
s2, preparing the Chinese herbal medicine extracting solution and a liquid culture medium according to the weight ratio of 20-30:800-1200, sterilizing at high pressure, and cooling to obtain the culture medium;
s3, respectively activating the bacillus and the lactic acid bacteria at 25-37 ℃ for 1-2min, inoculating the bacillus and the lactic acid bacteria into the culture medium obtained in S2 according to the proportion of 2-4%, and culturing at 25-37 ℃ at 30-100r/min until the logarithmic phase;
s4, inoculating the logarithmic phase zymocyte liquid into the residual culture medium prepared in the S2 according to the proportion of 1-5%, and culturing at 25-37 ℃ for 4-8d at 100-140 r/min;
s5, when the pH value of the fermentation liquor reaches 6.2-7.2 and the effective viable count reaches 2-10X 108CFU/mL, stopping fermentation, carrying out centrifugal separation, taking the precipitate and removing the supernatant;
s6, adding the precipitate into the Chinese herbal medicine extract with the same amount to obtain a suspension;
s7, mixing the following substances in parts by weight: 30-50 parts of suspension, 5-15 parts of trehalose and 800 parts of water, and then filtering and sterilizing to obtain a mixed solution;
and S8, taking the fruit and vegetable straw powder subjected to autoclaving, mixing the mixed liquid obtained in the step S7 with 30-90 parts by weight of the fruit and vegetable straw powder, and drying to obtain a finished product. The components of the Chinese herbal medicine extracting solution can inhibit the propagation of pathogenic bacteria and pests in soil, reduce the plant diseases and insect pests, promote the growth of flowers, improve the chlorophyll content of leaves, enhance the photosynthesis of plants, promote the synthesis of substances such as anthocyanin and the like, and prolong the ornamental period of the flowers.
The bacillus is one or more of bacillus subtilis, bacillus coagulans and bacillus pumilus. All adopt natural bacteria, do not cause potential threat to human body and environment, also do not harm human health.
The lactobacillus is one or more of Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus delbrueckii and Lactobacillus brevis. The common natural bacteria do not pose potential threats to human bodies and the environment.
The fruit and vegetable straw powder is prepared by grinding one or more of peanut shell, potato, tomato, eggplant, soybean and the like into powder. Is used for providing high-quality nutrients.
In S8, freeze drying is used. The freeze drying has the following advantages: 1. the food preservative is particularly suitable for heat-sensitive substances, can keep heat-sensitive components after the heat-sensitive materials are dried, and can preserve various levels of nutritional components in food, particularly more than 90% of vitamin C; 2. under vacuum and low temperature, the growth of microorganisms and enzyme action are inhibited; 3. extremely water-absorbing recovery; 4. because of the operation under vacuum, oxygen is very little, so some substances (such as grease) which are easy to oxidize are protected.
In S5, the centrifugation conditions are: the rotating speed is 6000r/min, and the time is 5-8 min.
Example 1:
s1, preparing a Chinese herbal medicine extracting solution, a liquid culture medium and fruit and vegetable straw powder: grinding herba plantaginis, flos Lonicerae and herba Portulacae into powder, adding water, boiling with slow fire, and filtering to obtain extractive solution; the liquid culture medium consists of the following raw materials by mass: molasses: 3 g, soybean meal: 20 g, corn steep liquor: 40 g, beef extract: 0.5g, sodium acetate: 3.0g, triammonium citrate: 1.0 g, dipotassium hydrogen phosphate: 1.0 g, magnesium sulfate: 0.1 g, manganese sulfate: 0.02 g, tween-80: 0.5g, distilled water: 700 g; the fruit and vegetable straw powder is prepared by grinding peanut shells and potatoes which have the same mass into powder;
s2, preparing the Chinese herbal medicine extract and a liquid culture medium according to the weight ratio of 20:800, sterilizing for 15min under high pressure, and cooling to obtain the culture medium;
s3, respectively activating the bacillus and the lactic acid bacteria at 25 ℃ for 1min, inoculating the bacillus and the lactic acid bacteria into the culture medium obtained in S2 according to the proportion of 2%, and culturing at 25 ℃ and 30r/min until the logarithmic phase;
s4, inoculating the logarithmic phase zymocyte liquid into the residual culture medium prepared in the S2 according to the proportion of 1%, and culturing for 4d at 25 ℃ at 100 r/min;
s5, when the pH value of the fermentation liquor reaches 6.2 and the effective viable count reaches 2 x 108Stopping fermentation at CFU/mL, performing centrifugal separation at 6000r/min for 5min, taking out precipitate, and removing supernatant;
s6, adding the precipitate into the Chinese herbal medicine extracting solution to obtain a suspension;
s7, mixing the following substances by mass: 30g of suspension, 5g of trehalose and 300 g of distilled water, and then filtering and sterilizing to obtain mixed liquor;
s8, taking the fruit and vegetable straw powder after autoclaving, mixing the mixed liquid obtained in S7 with the fruit and vegetable straw powder with the weight of 30-90 g, and then freezing and drying to obtain the finished product T1.
S9, burying 100g of T1 at the roots of the chrysanthemum two weeks before the flowering phase of the chrysanthemum, scoring by 1-10 points according to the vigorous growth degree, withering rate, more brilliant degree and lasting fragrance of the chrysanthemum, and recording the effective ornamental period.
Example 2:
s1, preparing a Chinese herbal medicine extracting solution, a liquid culture medium and fruit and vegetable straw powder: grinding Glycyrrhrizae radix, fructus Lycii, radix Angelicae sinensis and fructus crataegi into powder, adding water, boiling with slow fire, and filtering to obtain extractive solution; the liquid culture medium consists of the following raw materials by mass: molasses: 7g, soybean meal: 30g, corn steep liquor: 60g, beef extract: 1.5g, sodium acetate: 8.0g, triammonium citrate: 3.0g, dipotassium hydrogen phosphate: 3.0g, magnesium sulfate: 0.5g, manganese sulfate: 0.18g, tween-80: 1.5g, distilled water: 1300 g; the fruit and vegetable straw powder is prepared by grinding one or more of peanut shells, potatoes, tomatoes, eggplants, soybeans and the like with the same mass into powder;
s2, preparing the Chinese herbal medicine extract and a liquid culture medium according to the weight ratio of 30:1200, sterilizing for 30min under high pressure, and cooling to obtain the culture medium;
s3, respectively activating the bacillus and the lactic acid bacteria at 37 ℃ for 2min, inoculating the bacillus and the lactic acid bacteria into the culture medium obtained in S2 according to the proportion of 4%, and culturing at 37 ℃ and 100r/min until the logarithmic phase;
s4, inoculating the logarithmic phase zymocyte liquid into the residual culture medium prepared in the S2 according to the proportion of 5%, and culturing at 37 ℃ at 140r/min for 8 d;
s5, the pH value of the fermentation liquor reaches 7.2, and the effective viable count reaches 10 x 108Stopping fermentation at CFU/mL, performing centrifugal separation at 6000r/min for 8min, taking out precipitate, and removing supernatant;
s6, adding the precipitate into the Chinese herbal medicine extracting solution to obtain a suspension;
s7, mixing the following substances by mass: 50 g of suspension, 15 g of trehalose and 800 g of distilled water, and then filtering and sterilizing to obtain mixed liquor;
s8, taking the fruit and vegetable straw powder after autoclaving, mixing the mixed liquid obtained in S7 with the fruit and vegetable straw powder with the weight of 30-90 g, and then freezing and drying to obtain the finished product T2.
S9, burying 100g of T2 at the roots of the chrysanthemum two weeks before the flowering phase of the chrysanthemum, scoring by 1-10 points according to the vigorous growth degree, withering rate, more brilliant degree and lasting fragrance of the chrysanthemum, and recording the effective ornamental period.
Example 3:
s1, preparing a Chinese herbal medicine extracting solution, a liquid culture medium and fruit and vegetable straw powder: the Chinese herbal medicine extracting solution is prepared by grinding plantain, honeysuckle, purslane, liquorice, medlar, angelica and hawthorn which have the same mass into powder, adding water which is submerged in the medicinal powder, boiling the mixture with slow fire, and filtering the mixture to obtain extracting solution of the Chinese herbal medicine; the liquid culture medium consists of the following raw materials by mass: molasses: 5g, soybean meal: 25 g, corn steep liquor: 50 g, beef extract: 1 g, sodium acetate: 5.5 g, triammonium citrate: 2 g, dipotassium hydrogen phosphate: 2 g, magnesium sulfate: 0.3 g, manganese sulfate: 0.1 g, tween-80: 1 g, distilled water: 1000 g; the fruit and vegetable straw powder is prepared by grinding one or more of peanut shells, potatoes, tomatoes, eggplants, soybeans and the like with the same mass into powder;
s2, preparing the Chinese herbal medicine extract and a liquid culture medium according to the weight ratio of 25:1000, sterilizing for 15min under high pressure, and cooling to obtain the culture medium;
s3, respectively activating the bacillus and the lactic acid bacteria at 31 ℃ for 1.5min, inoculating the bacillus and the lactic acid bacteria into the culture medium obtained in S2 according to the proportion of 3%, and culturing at 31 ℃ and 65r/min until the logarithmic phase;
s4, inoculating the logarithmic phase zymocyte liquid into the residual culture medium prepared in the S2 according to the proportion of 3%, and culturing at 31 ℃ and 120r/min for 6 d;
s5, the pH value of the fermentation liquor reaches 6.8, and the effective viable count reaches 6 x 108Stopping fermentation at CFU/mL, performing centrifugal separation at 6000r/min for 6min, taking out precipitate, and removing supernatant;
s6, adding the precipitate into the Chinese herbal medicine extracting solution to obtain a suspension;
s7, mixing the following substances by mass: 40 g of suspension, 10 g of trehalose and 500 g of distilled water, and then filtering and sterilizing to obtain mixed liquor;
s8, taking the fruit and vegetable straw powder after autoclaving, mixing the mixed liquid obtained in S7 with the fruit and vegetable straw powder with the weight of 60g, and then freezing and drying to obtain the finished product T3.
S9, burying 100g of T3 at the roots of the chrysanthemum two weeks before the flowering phase of the chrysanthemum, scoring by 1-10 points according to the vigorous growth degree, withering rate, more brilliant degree and lasting fragrance of the chrysanthemum, and recording the effective ornamental period.
Example 4:
s1, preparing a Chinese herbal medicine extracting solution, a liquid culture medium and fruit and vegetable straw powder: the Chinese herbal medicine extracting solution is prepared by grinding plantain, honeysuckle, purslane, liquorice, medlar, angelica and hawthorn which have the same mass into powder, adding water which is submerged in the medicinal powder, boiling the mixture with slow fire, and filtering the mixture to obtain extracting solution of the Chinese herbal medicine; the liquid culture medium consists of the following raw materials by mass: molasses: 5g, soybean meal: 25 g, corn steep liquor: 50 g, beef extract: 1 g, sodium acetate: 5.5 g, triammonium citrate: 2 g, dipotassium hydrogen phosphate: 2 g, magnesium sulfate: 0.3 g, manganese sulfate: 0.1 g, tween-80: 1 g, distilled water: 1000 g; the fruit and vegetable straw powder is prepared by grinding one or more of peanut shells, potatoes, tomatoes, eggplants, soybeans and the like with the same mass into powder;
s2, preparing the Chinese herbal medicine extract and a liquid culture medium according to the weight ratio of 25:1000, sterilizing for 15min under high pressure, and cooling to obtain the culture medium;
s3, respectively activating the bacillus and the lactic acid bacteria at 31 ℃ for 1.5min, inoculating the bacillus and the lactic acid bacteria into the culture medium obtained in S2 according to the proportion of 3%, and culturing at 31 ℃ and 65r/min until the logarithmic phase;
s4, inoculating the logarithmic phase zymocyte liquid into the residual culture medium prepared in the S2 according to the proportion of 3%, and culturing at 31 ℃ and 120r/min for 6 d;
s5, the pH value of the fermentation liquor reaches 6.8, and the effective viable count reaches 6 x 108Stopping fermentation at CFU/mL, performing centrifugal separation at 6000r/min for 6min, taking out precipitate, and removing supernatant;
s6, adding the precipitate into the Chinese herbal medicine extracting solution to obtain a suspension;
s7, mixing the following substances by mass: 40 g of suspension, 10 g of trehalose and 500 g of distilled water, and then filtering and sterilizing to obtain mixed liquor;
s8, the mixed liquid obtained in the S7 is frozen and dried to prepare a finished product T4.
S9, burying 100g of T4 at the roots of the chrysanthemum two weeks before the flowering phase of the chrysanthemum, scoring by 1-10 points according to the vigorous growth degree, withering rate, more brilliant degree and lasting fragrance of the chrysanthemum, and recording the effective ornamental period.
Example 5:
s1, preparing a Chinese herbal medicine extracting solution, a liquid culture medium and fruit and vegetable straw powder: the Chinese herbal medicine extracting solution is prepared by grinding plantain, honeysuckle, purslane, liquorice, medlar, angelica and hawthorn which have the same mass into powder, adding water which is submerged in the medicinal powder, boiling the mixture with slow fire, and filtering the mixture to obtain extracting solution of the Chinese herbal medicine; the liquid culture medium consists of the following raw materials by mass: molasses: 5g, soybean meal: 25 g, corn steep liquor: 50 g, beef extract: 1 g, sodium acetate: 5.5 g, triammonium citrate: 2 g, dipotassium hydrogen phosphate: 2 g, magnesium sulfate: 0.3 g, manganese sulfate: 0.1 g, tween-80: 1 g, distilled water: 1000 g; the fruit and vegetable straw powder is prepared by grinding one or more of peanut shells, potatoes, tomatoes, eggplants, soybeans and the like with the same mass into powder;
s2, sterilizing the liquid culture medium for 15min under high pressure, and cooling to obtain a culture medium;
s3, respectively activating the bacillus and the lactic acid bacteria at 31 ℃ for 1.5min, inoculating the bacillus and the lactic acid bacteria into the culture medium obtained in S2 according to the proportion of 3%, and culturing at 31 ℃ and 65r/min until the logarithmic phase;
s4, inoculating the logarithmic phase zymocyte liquid into the residual culture medium prepared in the S2 according to the proportion of 3%, and culturing at 31 ℃ and 120r/min for 6 d;
s5, the pH value of the fermentation liquor reaches 6.8, and the effective viable count reaches 6 x 108Stopping fermentation at CFU/mL, performing centrifugal separation at 6000r/min for 6min, taking out precipitate, and removing supernatant;
s6, adding the precipitate into distilled water with the same amount to obtain a suspension;
s7, mixing the following substances by mass: 40 g of suspension, 10 g of trehalose and 500 g of distilled water, and then filtering and sterilizing to obtain mixed liquor;
s8, taking the fruit and vegetable straw powder after autoclaving, mixing the mixed liquid obtained in S7 with the fruit and vegetable straw powder with the weight of 60g, and then freezing and drying to obtain the finished product T5.
S9, burying 100g of T5 at the roots of the chrysanthemum two weeks before the flowering phase of the chrysanthemum, scoring by 1-10 points according to the vigorous growth degree, withering rate, more brilliant degree and lasting fragrance of the chrysanthemum, and recording the effective ornamental period.
Example 6:
s1, burying 100g of common fertilizer T6 at the roots of the chrysanthemum two weeks before the flowering phase of the chrysanthemum, scoring by 1-10 points according to the vigorous growth degree, the withering rate, the more brilliant degree and the lasting degree of the faint scent of the chrysanthemum, and recording the effective ornamental period.
The results of the experiment are shown in the following table:
the above-described embodiments should not be construed as limiting the scope of the invention, and any alternative modifications or alterations to the embodiments of the present invention will be apparent to those skilled in the art.
The present invention is not described in detail, but is known to those skilled in the art.
Claims (6)
1. A preparation method of a microecological preparation for prolonging the ornamental period of flowers is characterized by comprising the following steps: the method comprises the following steps:
s1, preparing Chinese herbal medicine extracting solution and liquid culture medium: the Chinese herbal medicine extracting solution is prepared by mixing plantain, honeysuckle and purslane with the same mass or mixing liquorice, medlar, angelica and hawthorn with the same mass or mixing plantain, honeysuckle, purslane, liquorice, medlar, angelica and hawthorn with the same mass, then grinding the mixture into medicinal powder, adding water which is submerged in the medicinal powder, boiling the mixture with slow fire, and filtering the mixture to obtain a filtrate to obtain the Chinese herbal medicine extracting solution; the liquid culture medium is composed of the following raw materials in parts by weight: molasses: 3-7 parts of soybean meal: 20-30 parts of corn steep liquor: 40-60 parts of beef extract: 0.5-1.5 parts, sodium acetate: 3.0-8.0 parts of triammonium citrate: 1.0-3.0 parts of dipotassium hydrogen phosphate: 1.0-3.0 parts, magnesium sulfate: 0.1-0.5 parts of manganese sulfate: 0.02-0.18 parts, tween-80: 0.5-1.5 parts of water: 700 portions and 1300 portions;
s2, preparing the Chinese herbal medicine extracting solution and a liquid culture medium according to the weight ratio of 20-30:800-1200, sterilizing at high pressure, and cooling to obtain the culture medium;
s3, respectively activating the bacillus and the lactic acid bacteria at 25-37 ℃ for 1-2min, inoculating the bacillus and the lactic acid bacteria into the culture medium obtained in S2 according to the proportion of 2-4%, and culturing at 25-37 ℃ at 30-100r/min until the logarithmic phase;
s4, inoculating the logarithmic phase zymocyte liquid into the residual culture medium prepared in the S2 according to the proportion of 1-5%, and culturing at 25-37 ℃ for 4-8d at 100-140 r/min;
the pH value of the fermentation liquor of S5 and S4 reaches 6.2-7.2, and the effective viable count reaches 2-10 x 108CFU/mL, stopping fermentation, performing centrifugal separation, taking a precipitate, and removing a supernatant;
s6, adding the precipitate into the Chinese herbal medicine extract with the same amount to obtain a suspension;
s7, mixing the following substances in parts by weight: 30-50 parts of suspension prepared by S6, 5-15 parts of trehalose and 800 parts of water, and then filtering and sterilizing to obtain mixed liquid;
and S8, taking the fruit and vegetable straw powder subjected to autoclaving, mixing the mixed liquid obtained in the step S7 with 30-90 parts by weight of the fruit and vegetable straw powder, and drying to obtain the finished product.
2. The method for preparing a microecological preparation for extending the ornamental period of flowers according to claim 1, wherein: the bacillus is one or more of bacillus subtilis, bacillus coagulans and bacillus pumilus.
3. The method for preparing a microecological preparation for extending the ornamental period of flowers according to claim 1, wherein: the lactobacillus is one or more of Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus delbrueckii and Lactobacillus brevis.
4. The method for preparing a microecological preparation for extending the ornamental period of flowers according to claim 1, wherein: the fruit and vegetable straw powder is prepared by grinding one or more of peanut shell, potato, tomato, eggplant and soybean into powder.
5. The method for preparing a microecological preparation for extending the ornamental period of flowers according to claim 1, wherein: in S8, freeze drying is used.
6. The method for preparing a microecological preparation for extending the ornamental period of flowers according to claim 1, wherein: in S5, the centrifugation conditions are: the rotating speed is more than 6000r/min, and the time is 5-8 min.
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| CN103155952A (en) * | 2013-02-28 | 2013-06-19 | 王茜 | Compound-type growth accelerating agents |
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