CN107232242A - A kind of preparation method for being used to extend the probiotics of flower ornamental phase - Google Patents
A kind of preparation method for being used to extend the probiotics of flower ornamental phase Download PDFInfo
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- CN107232242A CN107232242A CN201710326222.6A CN201710326222A CN107232242A CN 107232242 A CN107232242 A CN 107232242A CN 201710326222 A CN201710326222 A CN 201710326222A CN 107232242 A CN107232242 A CN 107232242A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
A kind of preparation method for being used to extend the probiotics of flower ornamental phase, comprises the following steps：The mixing of extracts of Chinese herbal medicine and fluid nutrient medium, extracts of Chinese herbal medicine and fluid nutrient medium, bacillus and lactic acid bacteria culture inoculation fermentation are prepared, taking precipitate is separated, prepares suspension, adds drying after other components finished product is made.The composition of extracts of Chinese herbal medicine of the present invention can suppress pathogen and insect breeding in soil, reduce pest and disease damage, promote flower growth, improve the chlorophyll content of blade, strengthen the photosynthesis of plant, promote the synthesis of the materials such as anthocyanidin, extend the viewing period of flowers；Containing nutriments such as abundant starch, polysaccharide, protein in Vegetable powder, nutriment necessary to being provided for the growth of plant promotes growing for plant；And by fermentation after, many macromoleculars are become into small molecule, the absorption of plant is more beneficial for.
Description
Technical field：
The present invention relates to a kind of preparation method for being used to extend the probiotics of flower ornamental phase.
Background technology：
The flower of delicate fragrance persistently, can mould mood, beautify the environment, increasingly deeper to be liked by consumers in general.While China
It is a flower planting and big export country, substantial amounts of flowers is had every year and are sold in international market, yet with deals with improperly
And the difference of environmental condition, the viewing period of flowers can be caused to shorten.Therefore, extend the viewing period of flowers, improve flowers product
Quality has caused the concern of people.At present, both at home and abroad for flowers viewing period extension method mainly include physical method and
Chemical method, although these methods can extend the viewing period of flowers, there is also some shortcomings.Physical method is mainly logical
Cross and change the growing environment condition of flowers to extend viewing period, such as：Reduce temperature, reduction illumination condition etc., but physical method institute
Need objective condition require it is higher, be difficult to control, very flexible, after processing the quality of product still it is difficult to ensure that.Chemical agent
Method is mainly by spraying chemical preservative to extend viewing period, and existing chemical method needs to use substantial amounts of agricultural chemicals medicine
Agent, causes residues of pesticides, pollutes environment, not disposable, directly or indirectly have impact on the physical and mental health of people.Prior art is to this
The not plan of solution.
The content of the invention：
The purpose of the present invention is used to extend flower ornamental aiming at the disadvantages mentioned above that prior art is present there is provided one kind
The preparation method of the probiotics of phase, it can suppress pathogen and insect breeding in soil, reduce pest and disease damage, promote flowers
Growth, improves the chlorophyll content of blade, strengthens the photosynthesis of plant, promotes the synthesis of the materials such as anthocyanidin, extends flowers
Viewing period, solve problems of the prior art.
The present invention is for the technical scheme that is used of solution above-mentioned technical problem：
A kind of preparation method for being used to extend the probiotics of flower ornamental phase, comprises the following steps：
S1, prepare extracts of Chinese herbal medicine and fluid nutrient medium：Extracts of Chinese herbal medicine is Asiatic plantain, honeysuckle, purslane, sweet
Grass, matrimony vine, Radix Angelicae Sinensis, hawthorn one or more are polished into powder, add the water for not having above-mentioned medicinal powder, and slow fire boils, taken after filtering
Filtrate obtains extracts of Chinese herbal medicine；Fluid nutrient medium is made up of the raw material of following parts by weight：Molasses：3-7g, dregs of beans：20-
30g, corn steep liquor：40-60g, beef extract：0.5-1.5g, sodium acetate：3.0-8.0g, Triammonium citrate：1.0-3.0g, phosphoric acid hydrogen
Dipotassium：1.0-3.0g, magnesium sulfate：0.1-0.5g, manganese sulfate：0.02-0.18g, Tween-80：0.5-1.5 parts, distilled water：
700-1300 parts；
S2, by extracts of Chinese herbal medicine and fluid nutrient medium by weight 20-30:800-1200 is prepared, autoclaving 15min
More than, culture medium is made after cooling；
S3, by bacillus and lactic acid bacteria respectively at 25 DEG C of -37 DEG C of activation 1-2min, be inoculated according to 2-4% ratio
In the culture medium that S2 is obtained, in 25 DEG C -37 DEG C, 30-100r/min, culture to logarithmic phase；
S4, logarithmic phase zymocyte liquid is seeded to made from S2 in remaining culture medium according to 1%-5% ratio respectively, in
25 DEG C -37 DEG C, 100-140r/min cultivates 4-8d；
S5, zymotic fluid pH value reach 6.2-7.2, treat that living bacteria count reaches 2-10*108CFU/mL, stop fermentation, centrifugation
Separation, taking precipitate abandons supernatant；
S6, sediment is added to equivalent extracts of Chinese herbal medicine obtains suspension；
S7, by the following material mixing of weight fraction：30-50 parts of suspension, 5-15 parts of trehalose, distilled water 300-800
Part, then filtration sterilization obtains mixed liquor；
S8, the fruits and vegetables powder of straw of autoclaving of learning from else's experience processing, the mixed liquor that S7 is obtained is 30-90 parts with parts by weight
Fruits and vegetables powder of straw mixing, then through drying finished product is made.
It is preferred that, the bacillus be bacillus subtilis, bacillus coagulans, bacillus pumilus in one kind or
It is several.
It is preferred that, the lactic acid bacteria is one kind in Lactobacillus casei, lactobacillus acidophilus, German-style lactobacillus, Lactobacillus brevis
Or it is several.
It is preferred that, fruits and vegetables powder of straw be the one or more such as peanut shell, potato, tomato, eggplant, soybean be polished into powder and
Into.
It is preferred that, using freeze-drying in S8.
It is preferred that, in S5, centrifugation condition is：Rotating speed 6000r/min, time 5-8min.
Compared with prior art, it is an advantage of the invention that：The composition of extracts of Chinese herbal medicine of the present invention can suppress in soil
Pathogen and insect breeding, reduce pest and disease damage, promote flower growth, improve the chlorophyll content of blade, strengthen the photosynthetic of plant
Effect, promotes the synthesis of the materials such as anthocyanidin, extends the viewing period of flowers；Contain abundant starch, polysaccharide, albumen in Vegetable powder
The nutriments such as matter, nutriment necessary to being provided for the growth of plant promotes growing for plant；And pass through hair
After ferment, many macromoleculars are become into small molecule, the absorption of plant is more beneficial for.
Embodiment：
For the technical characterstic for illustrating this programme can be understood, below by embodiment, the present invention is explained in detail
State.
S1, prepare extracts of Chinese herbal medicine and fluid nutrient medium：Extracts of Chinese herbal medicine is Asiatic plantain, honeysuckle, purslane, sweet
Grass, matrimony vine, Radix Angelicae Sinensis, hawthorn one or more are polished into powder, add the water for not having above-mentioned medicinal powder, and slow fire boils, taken after filtering
Filtrate obtains extracts of Chinese herbal medicine；Fluid nutrient medium is made up of the raw material of following parts by weight：Molasses：3-7 parts, dregs of beans：20-30
Part, corn steep liquor：40-60 parts, beef extract：0.5-1.5 parts, sodium acetate：3.0-8.0 part, Triammonium citrate：1.0-3.0 part, phosphorus
Sour hydrogen dipotassium：1.0-3.0 part, magnesium sulfate：0.1-0.5 parts, manganese sulfate：0.02-0.18 parts, Tween-80：0.5-1.5 parts, water：
700-1300 parts；
S2, by extracts of Chinese herbal medicine and fluid nutrient medium by weight 20-30:800-1200 is prepared, autoclaving, cooling
Culture medium is made afterwards；
S3, by bacillus and lactic acid bacteria respectively at 25 DEG C of -37 DEG C of activation 1-2min, be inoculated according to 2-4% ratio
In the culture medium that S2 is obtained, in 25 DEG C -37 DEG C, 30-100r/min, culture to logarithmic phase；
S4, logarithmic phase zymocyte liquid is seeded to made from S2 in remaining culture medium according to 1%-5% ratio respectively, in
25 DEG C -37 DEG C, 100-140r/min cultivates 4-8d；
S5, zymotic fluid pH value reach 6.2-7.2, treat that living bacteria count reaches 2-10*108CFU/mL, stop fermentation, centrifugation
Separation, taking precipitate abandons supernatant；
S6, sediment is added to equivalent extracts of Chinese herbal medicine obtains suspension；
S7, by the following material mixing of weight fraction：30-50 parts of suspension, 5-15 parts of trehalose, 300-800 parts of water,
Then filtration sterilization obtains mixed liquor；
S8, the fruits and vegetables powder of straw of autoclaving of learning from else's experience processing, the mixed liquor that S7 is obtained is 30-90 parts with parts by weight
Fruits and vegetables powder of straw mixing, then through drying finished product is made.The composition of extracts of Chinese herbal medicine of the present invention can suppress sick in soil
Opportunistic pathogen and insect breeding, reduce pest and disease damage, promote flower growth, improve the chlorophyll content of blade, strengthen the photosynthetic work of plant
With the synthesis of the material such as promotion anthocyanidin extends the viewing period of flowers.
The bacillus is the one or more in bacillus subtilis, bacillus coagulans, bacillus pumilus.Entirely
Portion uses nature bacterium, does not result in potential threat to human body and environment, will not also be detrimental to health.
The lactic acid bacteria is the one or more in Lactobacillus casei, lactobacillus acidophilus, German-style lactobacillus, Lactobacillus brevis.
Conventional natural bacterium, potential threat is not resulted in human body and environment.
Fruits and vegetables powder of straw is polished into powder for one or more such as peanut shell, potato, tomato, eggplant, soybean and formed.For
The nutriment of high-quality is provided.
Using freeze-drying in S8.It is freeze-dried advantage as follows：1st, heat-sensitive substance is particularly suitable for, temperature-sensitive can be made
Retain heat-sensitive ingredients after the dry materials of property, the nutritional ingredients at different levels in food can be preserved, especially to vitamin C, can be preserved
More than 90%；2nd, operated under vacuum and low temperature, the growth of microorganism and enzyme effect are suppressed；3rd, extremely water suction is restored；4、
Because operating under vacuo, oxygen is few, and therefore, some oxidizable materials (such as grease type) are protected.
In S5, centrifugation condition is：Rotating speed 6000r/min, time 5-8min.
Embodiment 1：
S1, prepare extracts of Chinese herbal medicine, fluid nutrient medium and fruits and vegetables powder of straw：Extracts of Chinese herbal medicine is car identical in quality
Preceding grass, honeysuckle, purslane are polished into powder, add the water for not having above-mentioned medicinal powder, and slow fire boils, and are taken after filtering during extract solution obtains
Herbal medicine extract solution；Fluid nutrient medium is made up of the raw material of following quality：Molasses：3 grams, dregs of beans：20 grams, corn steep liquor：40 grams, ox
Meat extract：0.5 gram, sodium acetate：3.0 grams, Triammonium citrate：1.0 grams, dipotassium hydrogen phosphate：1.0 grams, magnesium sulfate：0.1 gram, sulfuric acid
Manganese：0.02 gram, Tween-80：0.5 gram, distilled water：700 grams；Fruits and vegetables powder of straw is peanut shell identical in quality, potato polishing
Formed into powder；
S2, by extracts of Chinese herbal medicine and fluid nutrient medium by weight 20:800 prepare, autoclaving 15min, after cooling
Culture medium is made；
S3, bacillus and lactic acid bacteria respectively at 25 DEG C activated into 1min, the training that S2 is obtained is inoculated according to 2% ratio
Support in base, in 25 DEG C, 30r/min, culture to logarithmic phase；
S4, logarithmic phase zymocyte liquid is seeded to made from S2 in remaining culture medium according to 1% ratio respectively, in 25
DEG C, 100r/min cultivates 4d；
S5, zymotic fluid pH value reach 6.2, treat that living bacteria count reaches 2*108CFU/mL, stops fermentation, centrifuges, and turns
Fast 6000r/min, time 5min, taking precipitate abandons supernatant；
S6, sediment is added to extracts of Chinese herbal medicine obtains suspension；
S7, the material mixing by following quality：30 grams of suspension, 5 grams of trehalose, 300 grams of distilled water, then filtration sterilization
Obtain mixed liquor；
S8, the fruits and vegetables powder of straw of autoclaving of learning from else's experience processing, the mixed liquor that S7 is obtained is 30-90 grams with weight grams
Fruits and vegetables powder of straw mixing, it is then freeze-dried that finished product T1 is made.
S9, chrysanthemum florescence the last fortnight in chrysanthemum root buriing 100g T1, according to the vigorous degree of Dendranthema Growth, wither
Speed, the more bright-coloured degree of flower, the lasting degree of delicate fragrance carries out 1-10 points of marking, and records effective viewing period.
Embodiment 2：
S1, prepare extracts of Chinese herbal medicine, fluid nutrient medium and fruits and vegetables powder of straw：Extracts of Chinese herbal medicine is identical in quality sweet
Grass, matrimony vine, Radix Angelicae Sinensis, hawthorn are polished into powder, add the water for not having above-mentioned medicinal powder, and slow fire boils, and are taken after filtering during extract solution obtains
Herbal medicine extract solution；Fluid nutrient medium is made up of the raw material of following quality：Molasses：7 grams, dregs of beans：30 grams, corn steep liquor：60 grams, ox
Meat extract：1.5 grams, sodium acetate：8.0 grams, Triammonium citrate：3.0 grams, dipotassium hydrogen phosphate：3.0 grams, magnesium sulfate：0.5 gram, sulfuric acid
Manganese：0.18 gram, Tween-80：1.5 grams, distilled water：1300 grams；Fruits and vegetables powder of straw be peanut shell identical in quality, potato, kind
The one or more such as eggplant, eggplant, soybean are polished into powder and formed；
S2, by extracts of Chinese herbal medicine and fluid nutrient medium by weight 30:1200 prepare, autoclaving 30min, after cooling
Culture medium is made；
S3, bacillus and lactic acid bacteria respectively at 37 DEG C activated into 2min, the training that S2 is obtained is inoculated according to 4% ratio
Support in base, in 37 DEG C, 100r/min, culture to logarithmic phase；
S4, logarithmic phase zymocyte liquid is seeded to made from S2 in remaining culture medium according to 5% ratio respectively, in 37
DEG C, 140r/min cultivates 8d；
S5, zymotic fluid pH value reach 7.2, treat that living bacteria count reaches 10*108CFU/mL, stops fermentation, centrifuges,
Rotating speed 6000r/min, time 8min, taking precipitate abandons supernatant；
S6, sediment is added to extracts of Chinese herbal medicine obtains suspension；
S7, the material mixing by following quality：Then 50 grams of suspension, 15 grams of trehalose, 800 grams of distilled water crossed and filtered out
Bacterium obtains mixed liquor；
S8, the fruits and vegetables powder of straw of autoclaving of learning from else's experience processing, the mixed liquor that S7 is obtained is 30-90 grams with weight grams
Fruits and vegetables powder of straw is mixed, then freeze-dried that finished product T2 is made.
S9, chrysanthemum florescence the last fortnight in chrysanthemum root buriing 100g T2, according to the vigorous degree of Dendranthema Growth, wither
Speed, the more bright-coloured degree of flower, the lasting degree of delicate fragrance carries out 1-10 points of marking, and records effective viewing period.
Embodiment 3：
S1, prepare extracts of Chinese herbal medicine, fluid nutrient medium and fruits and vegetables powder of straw：Extracts of Chinese herbal medicine is car identical in quality
Preceding grass, honeysuckle, purslane, radix glycyrrhizae, matrimony vine, Radix Angelicae Sinensis, hawthorn are polished into powder, add the water for not having above-mentioned medicinal powder, and slow fire boils,
Extract solution is taken to obtain extracts of Chinese herbal medicine after filtering；Fluid nutrient medium is made up of the raw material of following quality：Molasses：5 grams, dregs of beans：
25 grams, corn steep liquor：50 grams, beef extract：1 gram, sodium acetate：5.5 grams, Triammonium citrate：2 grams, dipotassium hydrogen phosphate：2 grams, sulfuric acid
Magnesium：0.3 gram, manganese sulfate：0.1 gram, Tween-80：1 gram, distilled water：1000 grams；Fruits and vegetables powder of straw be peanut shell identical in quality,
The one or more such as potato, tomato, eggplant, soybean are polished into powder and formed；
S2, by extracts of Chinese herbal medicine and fluid nutrient medium by weight 25:1000 prepare, autoclaving 15min, after cooling
Culture medium is made；
S3, bacillus and lactic acid bacteria respectively at 31 DEG C activated into 1.5min, be inoculated in what S2 was obtained according to 3% ratio
In culture medium, in 31 DEG C, 65r/min, culture to logarithmic phase；
S4, logarithmic phase zymocyte liquid is seeded to made from S2 in remaining culture medium according to 3% ratio respectively, in 31
DEG C, 120r/min cultivates 6d；
S5, zymotic fluid pH value reach 6.8, treat that living bacteria count reaches 6*108CFU/mL, stops fermentation, centrifuges, and turns
Fast 6000r/min, time 6min, taking precipitate abandons supernatant；
S6, sediment is added to extracts of Chinese herbal medicine obtains suspension；
S7, the material mixing by following quality：Then 40 grams of suspension, 10 grams of trehalose, 500 grams of distilled water crossed and filtered out
Bacterium obtains mixed liquor；
S8, the fruits and vegetables powder of straw of autoclaving of learning from else's experience processing, the mixed liquor that S7 is obtained and the fruit that weight grams is 60 grams
Vegetable powder of straw is mixed, then freeze-dried that finished product T3 is made.
S9, chrysanthemum florescence the last fortnight in chrysanthemum root buriing 100g T3, according to the vigorous degree of Dendranthema Growth, wither
Speed, the more bright-coloured degree of flower, the lasting degree of delicate fragrance carries out 1-10 points of marking, and records effective viewing period.
Embodiment 4：
S1, prepare extracts of Chinese herbal medicine, fluid nutrient medium and fruits and vegetables powder of straw：Extracts of Chinese herbal medicine is car identical in quality
Preceding grass, honeysuckle, purslane, radix glycyrrhizae, matrimony vine, Radix Angelicae Sinensis, hawthorn are polished into powder, add the water for not having above-mentioned medicinal powder, and slow fire boils,
Extract solution is taken to obtain extracts of Chinese herbal medicine after filtering；Fluid nutrient medium is made up of the raw material of following quality：Molasses：5 grams, dregs of beans：
25 grams, corn steep liquor：50 grams, beef extract：1 gram, sodium acetate：5.5 gram, Triammonium citrate：2 grams, dipotassium hydrogen phosphate：2 grams, sulfuric acid
Magnesium：0.3 gram, manganese sulfate：0.1 gram, Tween-80：1 gram, distilled water：1000 grams；Fruits and vegetables powder of straw be peanut shell identical in quality,
The one or more such as potato, tomato, eggplant, soybean are polished into powder and formed；
S2, by extracts of Chinese herbal medicine and fluid nutrient medium by weight 25:1000 prepare, autoclaving 15min, after cooling
Culture medium is made；
S3, bacillus and lactic acid bacteria respectively at 31 DEG C activated into 1.5min, be inoculated in what S2 was obtained according to 3% ratio
In culture medium, in 31 DEG C, 65r/min, culture to logarithmic phase；
S4, logarithmic phase zymocyte liquid is seeded to made from S2 in remaining culture medium according to 3% ratio respectively, in 31
DEG C, 120r/min cultivates 6d；
S5, zymotic fluid pH value reach 6.8, treat that living bacteria count reaches 6*108CFU/mL, stops fermentation, centrifuges, and turns
Fast 6000r/min, time 6min, taking precipitate abandons supernatant；
S6, sediment is added to extracts of Chinese herbal medicine obtains suspension；
S7, the material mixing by following quality：Then 40 grams of suspension, 10 grams of trehalose, 500 grams of distilled water crossed and filtered out
Bacterium obtains mixed liquor；
S8, the mixed liquor for obtaining S7 are freeze-dried to be made finished product T4.
S9, chrysanthemum florescence the last fortnight in chrysanthemum root buriing 100g T4, according to the vigorous degree of Dendranthema Growth, wither
Speed, the more bright-coloured degree of flower, the lasting degree of delicate fragrance carries out 1-10 points of marking, and records effective viewing period.
Embodiment 5：
S1, prepare extracts of Chinese herbal medicine, fluid nutrient medium and fruits and vegetables powder of straw：Extracts of Chinese herbal medicine is car identical in quality
Preceding grass, honeysuckle, purslane, radix glycyrrhizae, matrimony vine, Radix Angelicae Sinensis, hawthorn are polished into powder, add the water for not having above-mentioned medicinal powder, and slow fire boils,
Extract solution is taken to obtain extracts of Chinese herbal medicine after filtering；Fluid nutrient medium is made up of the raw material of following quality：Molasses：5 grams, dregs of beans：
25 grams, corn steep liquor：50 grams, beef extract：1 gram, sodium acetate：5.5 grams, Triammonium citrate：2 grams, dipotassium hydrogen phosphate：2 grams, sulfuric acid
Magnesium：0.3 gram, manganese sulfate：0.1 gram, Tween-80：1 gram, distilled water：1000 grams；Fruits and vegetables powder of straw be peanut shell identical in quality,
The one or more such as potato, tomato, eggplant, soybean are polished into powder and formed；
S2, by fluid nutrient medium autoclaving 15min, after cooling be made culture medium；
S3, bacillus and lactic acid bacteria respectively at 31 DEG C activated into 1.5min, be inoculated in what S2 was obtained according to 3% ratio
In culture medium, in 31 DEG C, 65r/min, culture to logarithmic phase；
S4, logarithmic phase zymocyte liquid is seeded to made from S2 in remaining culture medium according to 3% ratio respectively, in 31
DEG C, 120r/min cultivates 6d；
S5, zymotic fluid pH value reach 6.8, treat that living bacteria count reaches 6*108CFU/mL, stops fermentation, centrifuges, and turns
Fast 6000r/min, time 6min, taking precipitate abandons supernatant；
S6, sediment is added to in equivalent distilled water to obtain suspension；
S7, the material mixing by following quality：Then 40 grams of suspension, 10 grams of trehalose, 500 grams of distilled water crossed and filtered out
Bacterium obtains mixed liquor；
S8, the fruits and vegetables powder of straw of autoclaving of learning from else's experience processing, the mixed liquor that S7 is obtained and the fruit that weight grams is 60 grams
Vegetable powder of straw is mixed, then freeze-dried that finished product T5 is made.
S9, chrysanthemum florescence the last fortnight in chrysanthemum root buriing 100g T5, according to the vigorous degree of Dendranthema Growth, wither
Speed, the more bright-coloured degree of flower, the lasting degree of delicate fragrance carries out 1-10 points of marking, and records effective viewing period.
Embodiment 6：
S1, chrysanthemum florescence the last fortnight chrysanthemum root buriing 100g common fertilizer T6, it is vigorous according to Dendranthema Growth
Degree, withers speed, the more bright-coloured degree of flower, the lasting degree of delicate fragrance carries out 1-10 points of marking, and records effective viewing period.
Experimental result see the table below：
Above-mentioned embodiment cannot function as limiting the scope of the invention, for the technology people of the art
For member, any alternate modification or conversion made to embodiment of the present invention are all fallen within protection scope of the present invention.
Part is not described in detail by the present invention, is the known technology of those skilled in the art of the present technique.
Claims (6)
1. a kind of preparation method for being used to extend the probiotics of flower ornamental phase, it is characterised in that：Comprise the following steps：
S1, prepare extracts of Chinese herbal medicine and fluid nutrient medium：Extracts of Chinese herbal medicine be Asiatic plantain, honeysuckle, purslane, radix glycyrrhizae,
Matrimony vine, Radix Angelicae Sinensis, hawthorn one or more are polished into powder, add the water for not having above-mentioned medicinal powder, and slow fire is boiled, and filtered fluid is taken after filtering
Obtain extracts of Chinese herbal medicine；Fluid nutrient medium is made up of the raw material of following parts by weight：Molasses：3-7 parts, dregs of beans：20-30 parts,
Corn steep liquor：40-60 parts, beef extract：0.5-1.5 parts, sodium acetate：3.0-8.0 part, Triammonium citrate：1.0-3.0 parts, phosphoric acid hydrogen
Dipotassium：1.0-3.0 part, magnesium sulfate：0.1-0.5 parts, manganese sulfate：0.02-0.18 parts, Tween-80：0.5-1.5 parts, water：700-
1300 parts；
S2, by extracts of Chinese herbal medicine and fluid nutrient medium by weight 20-30:800-1200 is prepared, autoclaving, is made after cooling
Obtain culture medium；
S3, by bacillus and lactic acid bacteria respectively at 25 DEG C of -37 DEG C of activation 1-2min, be inoculated in S2 according to 2-4% ratio and obtain
To culture medium in, in 25 DEG C -37 DEG C, 30-100r/min, culture to logarithmic phase；
S4, logarithmic phase zymocyte liquid is seeded to made from S2 in remaining culture medium according to 1%-5% ratio respectively, in 25
DEG C -37 DEG C, 100-140r/min cultivates 4-8d；
S5, S4 zymotic fluid pH value reach 6.2-7.2, and living bacteria count reaches 2-10*108CFU/mL, stops fermentation, centrifugation point
From taking precipitate abandons supernatant；
S6, sediment is added to in equivalent extracts of Chinese herbal medicine to obtain suspension；
S7, by the following material mixing of weight fraction：Suspension 30-50 parts made from S6,5-15 parts of trehalose, water 300-800
Part, then filtration sterilization obtains mixed liquor；
S8, the fruits and vegetables powder of straw of autoclaving of learning from else's experience processing, the mixed liquor that S7 is obtained and the fruits and vegetables that parts by weight are 30-90 parts
Powder of straw is mixed, then through being dried to obtain final finished.
2. a kind of preparation method for being used to extend the probiotics of flower ornamental phase according to claim 1, its feature
It is：The bacillus is the one or more in bacillus subtilis, bacillus coagulans, bacillus pumilus.
3. a kind of preparation method for being used to extend the probiotics of flower ornamental phase according to claim 1, its feature
It is：The lactic acid bacteria is the one or more in Lactobacillus casei, lactobacillus acidophilus, German-style lactobacillus, Lactobacillus brevis.
4. a kind of preparation method for being used to extend the probiotics of flower ornamental phase according to claim 1, its feature
It is：Fruits and vegetables powder of straw is polished into powder for one or more such as peanut shell, potato, tomato, eggplant, soybean and formed.
5. a kind of preparation method for being used to extend the probiotics of flower ornamental phase according to claim 1, its feature
It is：Using freeze-drying in S8.
6. a kind of preparation method for being used to extend the probiotics of flower ornamental phase according to claim 1, its feature
It is：In S5, centrifugation condition is：More than rotating speed 6000r/min, time 5-8min.
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