CN106305833A - Preparation for preventing anthracnose of rhizoma paris, and preparation method thereof - Google Patents
Preparation for preventing anthracnose of rhizoma paris, and preparation method thereof Download PDFInfo
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- CN106305833A CN106305833A CN201610701550.5A CN201610701550A CN106305833A CN 106305833 A CN106305833 A CN 106305833A CN 201610701550 A CN201610701550 A CN 201610701550A CN 106305833 A CN106305833 A CN 106305833A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
Abstract
The invention relates to the technical field of prevention of pests and diseases of traditional Chinese medicines, and particularly relates to a preparation for preventing anthracnose of rhizoma paris, and a preparation method thereof. The preparation contains the following raw materials: cymbidium sinense, rabdosia rubesens, pumpkin stems, thesium chinense, humifuse euphorbia herb, tea tree essential oil, chili stems and complex microorganisms. The preparation method comprises the following steps: firstly, mixing cymbidium sinense, humifuse euphorbia herb, chili stems with sweet water, then inoculating lactobacillus bulgaricus, performing constant-temperature soaking, then inoculating bacillus amyloliquefaciens and bacillus subtitles, performing secondary constant-temperature soaking, and filtering to obtain a soaking solution for later use; mixing thesium chinense, rabdosia rubesens and pumpkin stems, adding water for extraction, performing ultrasonic processing, and filtering to obtain extracting liquid for later use; evenly mixing the soaking solution, the extracting liquid and the tea tree essential oil, and evenly mixing with new high-fat film powder after performing oscillation and ultrasonic processing, so as to obtain the preparation. The preparation has balanced nutrients, is safe and non-toxic, and has better inhibition effect on harmful bacteria, especially has obvious inhibition effect on the bacillus anthracis.
Description
Technical field
The present invention relates to Chinese medicine techniques of pest control field, particularly relate to a kind of preparation preventing Rhizoma Paridis anthrax and
Preparation method.
Background technology
Rhizoma Paridis has another name called Rhizoma Paridis, belongs to liliaceous plant, another name: Rhizoma Paridis, Rhizoma Paridis, money Rhizoma Paridis, seven leaves, lamp-stand
Seven, Rhizoma Paridis, Omoto Nipponlily Rhizome, branch fresh idea, Carnis Rapanae thomasianae seven, Calanthe davidii Franhc., weight platform grass and Herba Senecionis Chrysanthemoidis etc., have heat-clearing and toxic substances removing, reducing swelling and alleviating pain,
Cool liver arresting convulsion health-care effect, Rhizoma Paridis has broad-spectrum antibacterial action, to dysentery bacterium, Bacillus typhi, escherichia coli, bacillus enteritidis,
Bacillus pyocyaneus, staphylococcus aureus, Hemolytic streptococcus, meningococcus etc. all have inhibitory action in various degree, especially
It is better than Rhizoma Coptidis to the inhibitory action of pyococcus；Asia influenza A virus there is stronger inhibitory action；Contained steroidal
Saponin and aminoacid have anti-snake venom action；Rhizoma Paridis glycosides has calmness, analgesic activity；Its water decoction or ethanol extraction have obvious town
Cough, antiasthmatic effect；Rhizoma Paridis powder has obvious anastalsis, additionally, also antitumor action, therefore, its medical value is increasingly
Coming into one's own, its crude drug consumption amount increases the most further, can not meet its demand day by day increased due to wild resource, because of
This, artificial growth Rhizoma Paridis becomes the necessary means solving Rhizoma Paridis scarcity of resources.
Constantly there is new pest and disease damage to occur under artificial cultivation condition, yield and the quality of Rhizoma Paridis medical material will be affected.At present,
The Major Diseases of Rhizoma Paridis has leaf spot, root rot, stem rot and anthrax, and wherein, anthrax harm is relatively universal, and easily causes people
Poultry suffers from altogether, becomes the potential threat of Rhizoma Paridis plantation, and it is perennial plant that its reason essentially consists in Rhizoma Paridis, and growth cycle is long, for a long time
It is planted in the continuous cropping mode in identical plot, is highly prone to infecting of pathogen.
Summary of the invention
For solving above-mentioned technical problem, the invention provides a kind of preparation preventing Rhizoma Paridis anthrax and preparation method thereof.
The present invention is achieved by the following technical programs.
A kind of preparation preventing Rhizoma Paridis anthrax, its raw material includes following parts by weight of component: Chinese cymbidium 0.1-1.5 part, winter insult
Grass 2-5 part, Caulis Cucurbitae 10-15 part, Herba Thesii 5-9 part, Herba Euphorbiae Humifusae 3-7 part, tea tree ethereal oil 8-13 part, Caulis Capsici 6-10 part, multiple
Closing bacterium 0.02-0.05 part, described compound bacteria is pressed by Bulgarian Lactobacillus, bacillus amyloliquefaciens and bacillus subtilis
Mass ratio (1.5-2): 1:(0.3-1) composition.
Further, its raw material includes following parts by weight of component: Chinese cymbidium 0.3-1.3 part, Rabdosia rubescens 2-3 part, Caulis Cucurbitae 11-
15 parts, Herba Thesii 6-8 part, Herba Euphorbiae Humifusae 4-6 part, tea tree ethereal oil 8-12 part, Caulis Capsici 7-9 part, compound bacteria 0.02-0.04 part.
Further, its raw material includes following parts by weight of component: Chinese cymbidium 0.8 part, Rabdosia rubescens 3 parts, Caulis Cucurbitae 13 parts, hundred
Stamen grass 7 parts, Herba Euphorbiae Humifusae 5 parts, tea tree ethereal oil 10 parts, Caulis Capsici 8 parts, compound bacteria 0.03 part.
Further, described compound bacteria is by Bulgarian Lactobacillus, bacillus amyloliquefaciens and bacillus subtilis
Bacterium 1.8:1:0.7 in mass ratio forms.
Further, described Herba Thesii is the flavonoid glycoside in Herba Thesii, mannitol to be extracted.
Its preparation method, comprises the following steps:
(1) after Chinese cymbidium, Herba Euphorbiae Humifusae, Caulis Capsici being mixed with sucrose solution, inoculate Bulgarian Lactobacillus, soak through constant temperature
After, inoculate bacillus amyloliquefaciens and bacillus subtilis, through secondary constant temperature immersion treatment, filter, obtain soak, standby；
(2) by after Herba Thesii, Rabdosia rubescens, Caulis Cucurbitae mixing, extracting in water, under the conditions of ultrasonic frequency is 53-58kHz
After supersound process 60-90s, filter, obtain extracting solution, standby；
(3) by soak, extracting solution, tea tree ethereal oil mixing, vibration 30-60min it is placed in constant temperature oscillator, then ultrasonic
Wave frequency is under the conditions of 30-35kHz after supersound process 10-15s, adds the new high fat film powder mixing of material gross weight 1-3%,
Obtain preparation.
Described sucrose solution, its Brix degree is 25-40%.
Described sucrose solution, its consumption is 2.5-3 times of soak gross weight.
Described constant temperature soaks, and its temperature is 28-32 DEG C.
Described constant temperature soaks, and its time is 1-5h.
Described extracting in water, its amount of water is 2-2.3 times of extract gross weight, and Extracting temperature is 60-80 DEG C, extracts
Time is 1-2h.
Described vibration, its temperature is 35 DEG C.
The method that the present invention also provides for preventing Rhizoma Paridis anthrax: before Rhizoma Paridis overwinter management, execute nitrogen phosphorus by 80-110kg/ mu
Potash fertilizer, wherein, the mass ratio of N P and K is nitrogen: potassium: phosphorus=10:2:1, then executes the preparation of the present invention by 10kg/ mu, has sprayed
Bi Hou, covers Semen Maydis powder by the amount of 12-15kg/ mu.
The beneficial effects of the present invention is:
The present invention, by understanding the growth characteristics of Rhizoma Paridis, utilizes Chinese cymbidium, Rabdosia rubescens, Caulis Cucurbitae, Herba Thesii, Radix seu Caulis Parthenocissi tricuspidatae
Grass, tea tree ethereal oil, Caulis Capsici are that raw material makes preparation, by the proportion design of science so that preparation is balanced in nutrition, equal in nutrition
Under conditions of weighing apparatus, enhance the inhibitory action to anthrax bacillus；The present invention is mixed with sucrose solution by Chinese cymbidium, Herba Euphorbiae Humifusae, Caulis Capsici,
Inoculation Bulgarian Lactobacillus, bacillus amyloliquefaciens and bacillus subtilis, provide nutrient for strain, in conjunction with temperature,
Dispensing and the regulation of vaccination ways so that bacterial activity strengthens, and then enhances the inhibitory action to anthrax bacillus；In conjunction with to hundred
Stamen grass, Rabdosia rubescens, Caulis Cucurbitae reasonably extract, on the premise of in Herba Thesii, flavonoid glycoside and mannitol exist so that the winter
Effective ingredient generation synergism in LINGCAO and Caulis Cucurbitae, enhances the preparation rejection ability to harmful bacteria, and then enhances
Inhibitory action to anthrax bacillus；Nutritional labeling, vibration and ultrasonic and parameter restriction in conjunction with tea tree ethereal oil so that extracting solution
Interact with soak so that effective ingredient interphase interaction, enhance probiotics vigor；In conjunction with new high fat film so that change
Learn disease-resistant disease-resistant with physics anti-combine in advance, and then improve the rejection ability to harmful bacteria.
Growth characteristics according to Rhizoma Paridis and absorption characteristic, the invention provides the side of a kind of effective anti-pre-Rhizoma Paridis anthrax
Method, compared with tradition prevention and controls, the method makes the healthy rate of Rhizoma Paridis improve about 35%, further promotes the growth of Rhizoma Paridis,
Make Rhizoma Paridis growth cycle shorten 8%, and the present invention is based on natural plants, the technique coordinating science so that preparation safety, nothing
Pollute.
Detailed description of the invention
Technical scheme is described further below, but claimed scope is not limited to described.
Embodiment 1
The present embodiment provides a kind of preparation preventing Rhizoma Paridis anthrax and preparation method thereof, specifically includes following steps:
1, raw material prepares
Include by weight: Chinese cymbidium 0.8kg, Rabdosia rubescens 3kg, Caulis Cucurbitae 13kg, Herba Thesii 7kg, Herba Euphorbiae Humifusae 5kg, Camellia sinensis
Quintessence oil 10kg, Caulis Capsici 8kg, compound bacteria 0.03kg；Wherein, compound bacteria is by Bulgarian Lactobacillus, bacillus amyloliquefaciens
And bacillus subtilis 1.8:1:0.7 in mass ratio composition；
2, the making of soak:
After Chinese cymbidium, Herba Euphorbiae Humifusae, Caulis Capsici and sucrose solution 34.5kg that Brix degree is 35% are mixed, inoculation Bulgaria lactic acid
Bacillus, after constant temperature soaks 2h under the conditions of being 30 DEG C in temperature, inoculates bacillus amyloliquefaciens and bacillus subtilis, continues
Under the conditions of Tong, constant temperature soaks 5h, filters, obtains soak, standby；
3, the making of extracting solution:
After Herba Thesii, Rabdosia rubescens, Caulis Cucurbitae mixing, add water 52kg, extracts 1h, ultrasonic under the conditions of temperature is 70 DEG C
Wave frequency is under the conditions of 56kHz after supersound process 70s, filters, obtains extracting solution, standby；
4, material mixing
By soak, extracting solution, tea tree ethereal oil mixing, it is placed in the constant temperature oscillator that temperature is 35 DEG C vibration 60min, then
Under the conditions of ultrasonic frequency is 33kHz after supersound process 15s, add the new high fat film powder mixing of material gross weight 1.5%,
Obtain preparation.
Embodiment 2
The present embodiment provides a kind of preparation preventing Rhizoma Paridis anthrax and preparation method thereof, specifically includes following steps:
1, raw material prepares
Include by weight: Chinese cymbidium 1.5kg, Rabdosia rubescens 2kg, Caulis Cucurbitae 15kg, Herba Thesii 5kg, Herba Euphorbiae Humifusae 3kg, Camellia sinensis
Quintessence oil 13kg, Caulis Capsici 6kg, compound bacteria 0.05kg；Wherein, compound bacteria is by Bulgarian Lactobacillus, bacillus amyloliquefaciens
And bacillus subtilis 1.8:1:0.7 in mass ratio composition；
2, the making of soak:
After Chinese cymbidium, Herba Euphorbiae Humifusae, Caulis Capsici and sucrose solution 31.5kg that Brix degree is 25% are mixed, inoculation Bulgaria lactic acid
Bacillus, after constant temperature soaks 4h under the conditions of being 28 DEG C in temperature, inoculates bacillus amyloliquefaciens and bacillus subtilis, continues
Under the conditions of Tong, constant temperature soaks 1h, filters, obtains soak, standby；
3, the making of extracting solution:
After Herba Thesii, Rabdosia rubescens, Caulis Cucurbitae mixing, add water 45kg, extracts 2h, ultrasonic under the conditions of temperature is 60 DEG C
Wave frequency is under the conditions of 58kHz after supersound process 80s, filters, obtains extracting solution, standby；
4, material mixing
By soak, extracting solution, tea tree ethereal oil mixing, it is placed in the constant temperature oscillator that temperature is 35 DEG C vibration 40min, then
Under the conditions of ultrasonic frequency is 35kHz after supersound process 12s, add the new high fat film powder mixing of material gross weight 3%, i.e.
Obtain preparation.
Embodiment 3
The present embodiment provides a kind of preparation preventing Rhizoma Paridis anthrax and preparation method thereof, specifically includes following steps:
1, raw material prepares
Include by weight: Chinese cymbidium 0.1kg, Rabdosia rubescens 5kg, Caulis Cucurbitae 10kg, Herba Thesii 9kg, Herba Euphorbiae Humifusae 7kg, Camellia sinensis
Quintessence oil 8kg, Caulis Capsici 10kg, compound bacteria 0.02kg；Wherein, compound bacteria is by Bulgarian Lactobacillus, bacillus amyloliquefaciens
And bacillus subtilis 1.8:1:1 in mass ratio composition；
2, the making of soak:
After Chinese cymbidium, Herba Euphorbiae Humifusae, Caulis Capsici and sucrose solution 43kg that Brix degree is 40% are mixed, inoculation Bulgaria lactic acid bar
Bacterium, after constant temperature soaks 5h under the conditions of being 30 DEG C in temperature, inoculates bacillus amyloliquefaciens and bacillus subtilis, continues same
Under the conditions of constant temperature soak 5h, filter, obtain soak, standby；
3, the making of extracting solution:
After Herba Thesii, Rabdosia rubescens, Caulis Cucurbitae mixing, add water 55kg, extracts 2h, ultrasonic under the conditions of temperature is 80 DEG C
Wave frequency is under the conditions of 53kHz after supersound process 60s, filters, obtains extracting solution, standby；
4, material mixing
By soak, extracting solution, tea tree ethereal oil mixing, it is placed in the constant temperature oscillator that temperature is 35 DEG C vibration 50min, then
Under the conditions of ultrasonic frequency is 33kHz after supersound process 10s, add the new high fat film powder mixing of material gross weight 2%, i.e.
Obtain preparation.
Embodiment 4
The present embodiment provides a kind of preparation preventing Rhizoma Paridis anthrax and preparation method thereof, specifically includes following steps:
1, raw material prepares
Include by weight: Chinese cymbidium 0.3kg, Rabdosia rubescens 3kg, Caulis Cucurbitae 11kg, Herba Thesii 6kg, Herba Euphorbiae Humifusae 4kg, Camellia sinensis
Quintessence oil 8kg, Caulis Capsici 7kg, compound bacteria 0.04kg；Wherein, compound bacteria by Bulgarian Lactobacillus, bacillus amyloliquefaciens and
Bacillus subtilis 2:1:0.3 in mass ratio forms；
2, the making of soak:
After Chinese cymbidium, Herba Euphorbiae Humifusae, Caulis Capsici and sucrose solution mixing 32kg that Brix degree is 30%, inoculation Bulgaria lactic acid bar
Bacterium, after constant temperature soaks 3h under the conditions of being 32 DEG C in temperature, inoculates bacillus amyloliquefaciens and bacillus subtilis, continues same
Under the conditions of constant temperature soak 2h, filter, obtain soak, standby；
3, the making of extracting solution:
After Herba Thesii, Rabdosia rubescens, Caulis Cucurbitae mixing, add water 60kg, extracts 1h, ultrasonic under the conditions of temperature is 65 DEG C
Wave frequency is under the conditions of 55kHz after supersound process 90s, filters, obtains extracting solution, standby；
4, material mixing
By soak, extracting solution, tea tree ethereal oil mixing, it is placed in the constant temperature oscillator that temperature is 35 DEG C vibration 30min, then
Under the conditions of ultrasonic frequency is 30kHz after supersound process 12s, add the new high fat film powder mixing of material gross weight 1%, i.e.
Obtain preparation.
Embodiment 5
The present embodiment provides a kind of preparation preventing Rhizoma Paridis anthrax and preparation method thereof, specifically includes following steps:
1, raw material prepares
Include by weight: Chinese cymbidium 1.3kg, Rabdosia rubescens 2kg, Caulis Cucurbitae 13kg, Herba Thesii 8kg, Herba Euphorbiae Humifusae 6kg, Camellia sinensis
Quintessence oil 12kg, Caulis Capsici 9kg, compound bacteria 0.02kg；Wherein, compound bacteria is by Bulgarian Lactobacillus, bacillus amyloliquefaciens
And bacillus subtilis 1.8:1:0.3 in mass ratio composition；
Its preparation method is with embodiment 2.
Embodiment 6
The present embodiment provides a kind of preparation preventing Rhizoma Paridis anthrax and preparation method thereof, specifically includes following steps:
1, raw material prepares
Include by weight: Chinese cymbidium 0.09kg, Rabdosia rubescens 1.5kg, Caulis Cucurbitae 9kg, Herba Thesii 4kg, Herba Euphorbiae Humifusae 2kg, tea
Tree quintessence oil 7.5kg, Caulis Capsici 5kg, compound bacteria 0.01kg；Wherein, compound bacteria is by Bulgarian Lactobacillus, solution starch spore bar
Bacterium and bacillus subtilis 1.8:1:0.7 in mass ratio forms；
Its preparation method is with embodiment 1.
Embodiment 7
The present embodiment provides a kind of preparation preventing Rhizoma Paridis anthrax and preparation method thereof, specifically includes following steps:
1, raw material prepares
Include by weight: Chinese cymbidium 2kg, Rabdosia rubescens 6kg, Caulis Cucurbitae 15.5kg, Herba Thesii 9.5kg, Herba Euphorbiae Humifusae 8kg, tea
Tree quintessence oil 14kg, Caulis Capsici 11kg, compound bacteria 0.06kg；Wherein, compound bacteria is by Bulgarian Lactobacillus, solution starch spore bar
Bacterium and bacillus subtilis 2:1:0.7 in mass ratio forms；
Its preparation method is with embodiment 3.
Claims (10)
1. the preparation preventing Rhizoma Paridis anthrax, it is characterised in that its raw material includes following parts by weight of component: Chinese cymbidium 0.1-
1.5 parts, Rabdosia rubescens 2-5 part, Caulis Cucurbitae 10-15 part, Herba Thesii 5-9 part, Herba Euphorbiae Humifusae 3-7 part, tea tree ethereal oil 8-13 part, Caulis Capsici
6-10 part, compound bacteria 0.02-0.05 part, described compound bacteria is by Bulgarian Lactobacillus, bacillus amyloliquefaciens and hay
Bacillus cereus (1.5-2): 1:(0.3-1 in mass ratio) composition.
2. the preparation preventing Rhizoma Paridis anthrax as claimed in claim 1, it is characterised in that its raw material includes following weight portion group
Point: Chinese cymbidium 0.3-1.3 part, Rabdosia rubescens 2-3 part, Caulis Cucurbitae 11-15 part, Herba Thesii 6-8 part, Herba Euphorbiae Humifusae 4-6 part, tea tree ethereal oil 8-
12 parts, Caulis Capsici 7-9 part, compound bacteria 0.02-0.04 part.
3. the preparation preventing Rhizoma Paridis anthrax as claimed in claim 1 or 2, it is characterised in that its raw material includes following weight
Part component: Chinese cymbidium 0.8 part, Rabdosia rubescens 3 parts, Caulis Cucurbitae 13 parts, Herba Thesii 7 parts, Herba Euphorbiae Humifusae 5 parts, tea tree ethereal oil 10 parts, Caulis Capsici
8 parts, compound bacteria 0.03 part.
4. as described in any one of claim 1-3 prevention Rhizoma Paridis anthrax preparation, it is characterised in that described compound bacteria by
Bulgarian Lactobacillus, bacillus amyloliquefaciens and bacillus subtilis 1.8:1:0.7 in mass ratio forms.
5. the preparation preventing Rhizoma Paridis anthrax as claimed in claim 1, it is characterised in that described Herba Thesii is by Herba Thesii
In flavonoid glycoside, mannitol extract.
6. the preparation method of the preparation of the prevention Rhizoma Paridis anthrax as described in any one of claim 1-3, it is characterised in that
Comprise the following steps:
(1), after Chinese cymbidium, Herba Euphorbiae Humifusae, Caulis Capsici being mixed with sucrose solution, Bulgarian Lactobacillus is inoculated, after constant temperature soaks, then
Inoculation bacillus amyloliquefaciens and bacillus subtilis, through secondary constant temperature immersion treatment, filter, obtain soak, standby；
(2) after Herba Thesii, Rabdosia rubescens, Caulis Cucurbitae being mixed, extracting in water, ultrasonic under the conditions of ultrasonic frequency is 53-58kHz
After process, filter, obtain extracting solution, standby；
(3) by soak, extracting solution, tea tree ethereal oil mixing, vibration 30-60min it is placed in constant temperature oscillator, then at ultrasound wave frequency
Rate is under the conditions of 30-35kHz after supersound process, adds the new high fat film powder mixing of material gross weight 1-3%, obtains preparation.
7. the preparation method of the preparation of prevention Rhizoma Paridis anthrax as claimed in claim 6, it is characterised in that described sucrose solution,
Its Brix degree is 25-40%.
8. the preparation method of the preparation of prevention Rhizoma Paridis anthrax as claimed in claim 6, it is characterised in that described constant temperature leaching
Bubble, its temperature is 28-32 DEG C.
9. the preparation method of the preparation of prevention Rhizoma Paridis anthrax as claimed in claim 6, it is characterised in that described adding water carries
Taking, its amount of water is 2-2.3 times of extract gross weight, and Extracting temperature is 60-80 DEG C, and extraction time is 1-2h.
10. the preparation method of the preparation of prevention Rhizoma Paridis anthrax as claimed in claim 6, it is characterised in that described vibration,
Its temperature is 35 DEG C.
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Cited By (6)
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| CN107439615A (en) * | 2017-07-21 | 2017-12-08 | 广西桂平市蒙圩镇柱强种养专业合作社 | Preparation of Paris polyphylla pest and disease damage and preparation method thereof can effectively be prevented and treated |
| CN107646902A (en) * | 2017-08-22 | 2018-02-02 | 紫云苗族布依族自治县华鹏农业科技开发有限公司 | A kind of composition for treating macaque peach blight |
| CN109757525A (en) * | 2019-03-18 | 2019-05-17 | 云南明远中药材种植有限公司 | A kind of Chinese medicine and preparation method thereof for preventing and treating Paris polyphylla root-rot stem rot gray mold |
| CN109757533A (en) * | 2019-02-01 | 2019-05-17 | 盐城工学院 | A kind of biological pesticide and preparation method thereof |
| CN114916563A (en) * | 2022-05-05 | 2022-08-19 | 山东农业大学 | Bacillus amyloliquefaciens and application of synergist thereof |
| CN115537363A (en) * | 2022-11-24 | 2022-12-30 | 云南省农业科学院农业环境资源研究所 | Bacillus subtilis, microbial inoculum, biological agent and application thereof |
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| CN107646902A (en) * | 2017-08-22 | 2018-02-02 | 紫云苗族布依族自治县华鹏农业科技开发有限公司 | A kind of composition for treating macaque peach blight |
| CN109757533A (en) * | 2019-02-01 | 2019-05-17 | 盐城工学院 | A kind of biological pesticide and preparation method thereof |
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| CN114916563A (en) * | 2022-05-05 | 2022-08-19 | 山东农业大学 | Bacillus amyloliquefaciens and application of synergist thereof |
| CN114916563B (en) * | 2022-05-05 | 2023-02-03 | 山东农业大学 | Bacillus amyloliquefaciens and application of synergist thereof |
| CN115537363A (en) * | 2022-11-24 | 2022-12-30 | 云南省农业科学院农业环境资源研究所 | Bacillus subtilis, microbial inoculum, biological agent and application thereof |
| CN115537363B (en) * | 2022-11-24 | 2023-03-10 | 云南省农业科学院农业环境资源研究所 | Bacillus subtilis, microbial inoculum, biological agent and application thereof |
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