CN107509732A - A kind of application of epiphysin in rape Freezing stress is alleviated - Google Patents
A kind of application of epiphysin in rape Freezing stress is alleviated Download PDFInfo
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- CN107509732A CN107509732A CN201710825259.3A CN201710825259A CN107509732A CN 107509732 A CN107509732 A CN 107509732A CN 201710825259 A CN201710825259 A CN 201710825259A CN 107509732 A CN107509732 A CN 107509732A
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- epiphysin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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Abstract
The invention discloses a kind of application of epiphysin in Brassica Napus Seedling freeze injury is alleviated, its step is：1）According to weight than preparing the aqueous solution containing epiphysin, it is dissolved according to weight than weighing epiphysin with the weight of water in corresponding water；2）The blade face of the Brassica Napus Seedling of growth is sprayed, every two alms bowl sprays 20ml, makes plant leaf tow sides moistening uniform, is cultivated after having sprayed under normal temperature, and then 4 DEG C of each processing 1 hour of culture one day, 2 DEG C and 1 DEG C, are finally cultivated 2 hours under the conditions of 2 DEG C；3）Brassica Napus Seedling blade face is sprayed, sprayed at dusk, sprays frequency once a day, continuous three days, always to spray number as three times, the amount of solution that each every plant of seedling sprays is 5mL, making plant blade face moistening uniform.Method is simple, strong operability, and it can effectively alleviate the injury that freeze injury is brought to Brassica Napus Seedling, significantly improves the freezing tolerance of rape.
Description
Technical field
The present invention relates to Rape-seed production field, is more particularly to a kind of use of epiphysin in rape Freezing stress is alleviated
On the way.
Background technology
Rape is Chinese unique oil crops that survive the winter, and the temperature of suitable growth is 10~20 DEG C, too high or too low
Growing for rape is had undesirable effect.When winter temperature is down to below 3 DEG C, growth of rape is stagnated, lower temperature
When, rape leaf starts performance and endured cold symptom, causes plant dead when serious.At present, to meet the needs of production, rape
The cultivation date gradually postpones, and causes also not up to required condition of surviving the winter just to have been enter into winter, the winter resistance of rape is proposed
Higher requirement.Therefore how research improves the frost resistance of rape, helps Brassica Napus Seedling safe overwintering to be particularly important.
Adaptation of the crop to adverse circumstance is restricted by two kinds of factors of hereditary capacity and crop body physiological situation, physiological status
With crop hormone in vivo close relation.Strengthen resistivity of the crop to adverse circumstance using plant growth regulator, into
One of important means for the degeneration-resistant border cultivation of current crop.For example, there are some researches show ABA and GA are related to plant cold resistance,
External source, which applies, can effectively improve plant cold resistance.Epiphysin (melatonin, MT) is new indole parahormone, and chemical name is
Melatonin, it is widely present in animals and plants.Epiphysin has multiple functions in plant, including regulation is planted
Thing grows, and such as promotes the growth of lateral root, changes the florescence, participates in Chlorophyll synthesis and Delaying Leaf-Senescence.Research at present
Think, epiphysin is mainly due to the removing that it participates in active oxygen to the adjustment effect of plant stress-resistance.Relevant epiphysin is to freeze injury
The relieving effect of the lower Brassica Napus Seedling of stress has not been reported, and this research takes external source addition epiphysin processing to alleviate low temperature to oil
The injury of dish seedling, to alleviate rape freeze injury, promote Brassica Napus Seedling safe overwintering, open a new way.
The content of the invention
The purpose of the present invention is the application for being the provision of a kind of epiphysin in Brassica Napus Seedling Freezing stress is alleviated, method
Simply, strong operability, it can effectively alleviate the injury that freeze injury is brought to Brassica Napus Seedling, significantly improve the freeze proof energy of rape
Power.
In order to realize above-mentioned purpose, the present invention uses following technical measures：
A kind of application of epiphysin in Brassica Napus Seedling freeze injury is alleviated, the structural formula of the epiphysin are：
Its step is：
1) it is commercially available prod than preparing the aqueous solution (0.5-2.0g/L) containing epiphysin, epiphysin according to weight, solid,
According to weight (0.5 of the weight than weighing epiphysin and water:1000-2:1000) it is dissolved in corresponding water.
2) blade face of the Brassica Napus Seedling of growth 19-21 days is sprayed, every two alms bowl sprays 20ml (4, an alms bowl seedling), makes
Plant leaf tow sides moistening is uniform.Cultivate at 19-22 DEG C after having sprayed 1-3 days, then cultivate one day, 2 DEG C and 1 DEG C for 4 DEG C
Each processing 1 hour.Finally cultivated 2 hours under the conditions of -2 DEG C.
3) Brassica Napus Seedling blade face is sprayed, the general dusk (18:00 or so) sprayed, spray frequency as one day one
It is secondary, continuous three days, number was always sprayed as three times.The amount of solution that each every plant of seedling sprays is 5mL, makes plant blade face moistening equal
It is even, patience of the Brassica Napus Seedling to low temperature can be significantly improved, significantly improves the survival of Brassica Napus Seedling under Freezing stress
Rate.
The present invention compared with prior art, has advantages below and effect：
Serotonin is applied to alleviate in rape salt damage stress by the present invention, and application method is simple and easy, workable,
This method can significantly mitigate injury of the salt damage stress to rapeseed plants, it will be apparent that improve the energy of rapeseed plants resistance salt damage stress
Power.Under salt damage stress conditions, by spray serotonin solution can Brassica Napus Seedling root long, stem length, ground fresh weight,
Upper dry weight, underground fresh weight and underground dry weight increase respectively compared to increment for the Brassica Napus Seedling for not spraying serotonin
7.2%-21.7%, 3.6%-9.3%, 10.4%-38.7%, 15.4%-45.5%13.1%-36.6% and highest increase
47.1%.
Epiphysin is used to alleviate in rape Freezing stress by the present invention, and application method is simple and easy, workable, the party
Method can significantly improve Brassica Napus Seedling freezing tolerance.Compared with control group, pre-processed by epiphysin, rape is in Freezing stress
Afterwards, survival rate can be promoted to 50%-87.5% from 25%, and survival rate improves 2-3 times.
Brief description of the drawings
Fig. 1 is a kind of survival rate signal of the Brassica Napus Seedling after epiphysin pretreatment can significantly improve Freezing stress
Figure.
Control group：Spray water；Treatment group 1：Spray 0.5:1000 epiphysin solution；
Treatment group 2：Spray 1.0:1000 epiphysin solution；
Treatment group 3：Spray 2.0:1000 epiphysin solution.
Embodiment
The present invention is expanded on further with reference to specific embodiment, but the present invention is not limited to following examples.
Embodiment 1：
With reference to embodiment, the present invention will be further described, material therefor, and such as epiphysin, vermiculite soil is city
Sell, mill water culture nutrient solution is formulated by Huo Gelan nutrient solution prescriptions.
A kind of application of epiphysin in Brassica Napus Seedling Freezing stress is alleviated, its step are：
1) table 1 (water and epiphysin weight proportion) weight is first according to than preparing the aqueous solution containing epiphysin, epiphysin
For commercially available prod, solid, weigh corresponding weight with epiphysin weight proportion according to the water of table 1 and be dissolved in corresponding water；
2) warp layer cloth is enclosed in rectangular plastic magazine, is tightened with rubber band, is placed in equipped with 1/4 Glan nutrient solution suddenly
Hydroponic box in, liquid level touches gauze, chooses full seed, is arranged on gauze, is placed in 22 or 23 or 24 or 25 DEG C of trainings
Support in room, 16/8h brightness culture 7 days, then select the consistent Brassica Napus Seedling of growing way, be caught in plastic plate with holes, then put
In being accompanied equipped with 1 in the hydroponic box of Huo Gelan nutrient solutions, the photoperiod is placed on as in 16h on daytime, 25 DEG C/night 8h, 22 DEG C of greenhouse
Culture 7 days.After one week, choose grow consistent Brassica Napus Seedling move into fill vermiculite soil the small side's alms bowl of black (10 × 10 ×
In 8.5cm), per 4 seedlings of alms bowl, cultivated 7 days in the photoperiod is 16h on daytime, 25 DEG C/night 8h, 22 DEG C of greenhouse, in case place
Reason；
3) choose the growing way unanimously growth Brassica Napus Seedling of 19 or 20 or 21 days, be divided into 4 groups, every group of two alms bowls, carry out respectively with
Lower processing：
4) control group, water is sprayed；Treatment group 1：Spray 0.5:1000 epiphysin solution；Treatment group 2：Spray 1:1000
Epiphysin solution；Treatment group 3：Spray 2.0:1000 epiphysin solution.Every plant of seedling sprays 5ml, sprays frequency as one day one
It is secondary, number is always sprayed as three times；
5) and then 4 DEG C are continued each processing 1 hour of culture one day, 2 DEG C and 1 DEG C, are finally handled 2 hours under the conditions of -2 DEG C；
6) seedling is cultivated into 1h in 15 DEG C after processing terminates and observes its wilting degree, counts survival rate of seedling.
The treatment fluid of table 1 specifically matches：
The rape survival rate statistical form of table 2
As shown in Table 2：Compared with control group, pre-processed by epiphysin, for rape after Freezing stress, survival rate can be from
25% is promoted to 50%-87.5%, and survival rate improves 2-3 times.
Claims (1)
1. a kind of application of epiphysin in Brassica Napus Seedling freeze injury is alleviated, its step are：
1）According to weight than preparing the aqueous solution 0.5-2.0g/L containing epiphysin, according to weight of the weight than weighing epiphysin and water
Amount：0.5:1000-2:1000 are dissolved in corresponding water；
2）The blade face of the growth Brassica Napus Seedling of 19-21 days is sprayed, every two alms bowl sprays 20ml：One 4, alms bowl seedling, makes plant
Blade tow sides moistening is uniform, is cultivated after having sprayed under normal temperature 1-3 days, then 4 DEG C of each processing 1 of culture one day, 2 DEG C and 1 DEG C
Hour, finally cultivated 2 hours under the conditions of -2 DEG C；
3）Brassica Napus Seedling blade face is sprayed, sprayed at dusk, sprays frequency once a day, continuous three days, always to spray
Number makes plant blade face moistening uniform for three times, the amount of solution that each every plant of seedling sprays is 5 mL.
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| CN201710825259.3A CN107509732B (en) | 2017-09-14 | 2017-09-14 | Application of melatonin in relieving freezing injury stress of rape |
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| CN201710825259.3A CN107509732B (en) | 2017-09-14 | 2017-09-14 | Application of melatonin in relieving freezing injury stress of rape |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108849904A (en) * | 2018-08-02 | 2018-11-23 | 吉林省农业科学院 | Low-temperature resistance plant growth regulator and its preparation method and application |
| CN109496711A (en) * | 2018-12-29 | 2019-03-22 | 中国农业科学院油料作物研究所 | A kind of serotonin is alleviating the application in rape freeze injury |
| CN109673456A (en) * | 2019-01-06 | 2019-04-26 | 山东农业大学 | A method of chrysanthemum winter resistance is improved using epiphysin |
| CN109793004A (en) * | 2019-01-04 | 2019-05-24 | 深圳诺普信农化股份有限公司 | Suspension seed-coating agent and its preparation method and application |
| CN110946140A (en) * | 2019-12-11 | 2020-04-03 | 广东省生物工程研究所（广州甘蔗糖业研究所） | Application of melatonin in increasing sugar content of sugarcane |
| CN111615884A (en) * | 2019-02-27 | 2020-09-04 | 中国农业科学院油料作物研究所 | Application of 5-hydroxytryptamine in relieving low-temperature stress of rape in germination period |
| CN111615883A (en) * | 2019-02-27 | 2020-09-04 | 中国农业科学院油料作物研究所 | Application of melatonin in relieving low-temperature stress of rape in germination period |
| CN114938755A (en) * | 2022-07-05 | 2022-08-26 | 宁夏大学 | Method for improving anti-late spring cold freezing damage capability of cabernet sauvignon seedlings |
| CN115504828A (en) * | 2022-09-02 | 2022-12-23 | 广东省农业科学院蔬菜研究所 | Method for improving low calcium stress resistance of wax gourds |
Citations (1)
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| CN103875673A (en) * | 2014-03-24 | 2014-06-25 | 中国农业科学院农业质量标准与检测技术研究所 | Novel application of melatonin to promotion of plant growth |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108849904A (en) * | 2018-08-02 | 2018-11-23 | 吉林省农业科学院 | Low-temperature resistance plant growth regulator and its preparation method and application |
| CN109496711A (en) * | 2018-12-29 | 2019-03-22 | 中国农业科学院油料作物研究所 | A kind of serotonin is alleviating the application in rape freeze injury |
| CN109793004A (en) * | 2019-01-04 | 2019-05-24 | 深圳诺普信农化股份有限公司 | Suspension seed-coating agent and its preparation method and application |
| CN109673456A (en) * | 2019-01-06 | 2019-04-26 | 山东农业大学 | A method of chrysanthemum winter resistance is improved using epiphysin |
| CN111615884A (en) * | 2019-02-27 | 2020-09-04 | 中国农业科学院油料作物研究所 | Application of 5-hydroxytryptamine in relieving low-temperature stress of rape in germination period |
| CN111615883A (en) * | 2019-02-27 | 2020-09-04 | 中国农业科学院油料作物研究所 | Application of melatonin in relieving low-temperature stress of rape in germination period |
| CN111615884B (en) * | 2019-02-27 | 2022-03-08 | 中国农业科学院油料作物研究所 | Application of 5-hydroxytryptamine in relieving low-temperature stress of rape in germination period |
| CN110946140A (en) * | 2019-12-11 | 2020-04-03 | 广东省生物工程研究所（广州甘蔗糖业研究所） | Application of melatonin in increasing sugar content of sugarcane |
| CN114938755A (en) * | 2022-07-05 | 2022-08-26 | 宁夏大学 | Method for improving anti-late spring cold freezing damage capability of cabernet sauvignon seedlings |
| CN115504828A (en) * | 2022-09-02 | 2022-12-23 | 广东省农业科学院蔬菜研究所 | Method for improving low calcium stress resistance of wax gourds |
| CN115504828B (en) * | 2022-09-02 | 2023-09-05 | 广东省农业科学院蔬菜研究所 | Method for improving low-calcium stress resistance of white gourd |
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