CN101984799B - Method for continuously propagating large amount of Cnaphalocrocis medinalis in field - Google Patents
Method for continuously propagating large amount of Cnaphalocrocis medinalis in field Download PDFInfo
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- CN101984799B CN101984799B CN201010537023A CN201010537023A CN101984799B CN 101984799 B CN101984799 B CN 101984799B CN 201010537023 A CN201010537023 A CN 201010537023A CN 201010537023 A CN201010537023 A CN 201010537023A CN 101984799 B CN101984799 B CN 101984799B
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Abstract
The invention provides a method for continuously propagating a large amount of Cnaphalocrocis medinalis in field, which comprises the following steps: (1) building a nylon net room which is 40 meters long and 8 meters wide in a field, dividing the nylon net room into four parts transversely, and continuously sowing seeds or transplanting rice seedlings in the independent divided parts every 25 to30 days; (2) performing normal field management; (3) inoculating adults of Cnaphalocrocis medinalis into the first isolated part in a high tillering period of rice, and finally allowing 10 to 20 percent of senile larvae or pupae to propagate in the first part; and (4), in the peak emergence period of adults, opening a second isolated nylon net adjacent to the first isolated part for keeping the Cnaphalocrocis medinalis population in the net room going. Thus, larvae of the same age used for doing experiments can be bred continuously and in large amount and supplied continuously.
Description
Technical field
The present invention relates to insect and raise and field of pest control, the method for a large amount of continuously breeding rice leaf roller in a kind of field is provided.
Background technology
Rice leaf roller Cnaphalocrocis medinalis (Guenee) belongs to migratory pest, and the annual rice district, China south of moving into from Vietnam and other places is one of most important insect (Shepard etc., 1995 on China's south rice; Cheng Jiaan, 1996).This worm is serious day by day in China's generation since 2000, area takes place every year surpass 2,000 ten thousand hectares, 760,000 tons of average annual grain losses.Particularly 2007, receive the influence of factors such as worm source radix, climatic factor and planting conditions, large tracts of land takes place rice leaf roller in rice district, China south, and 2,530 ten thousand hectares of areas take place, and is that the maximum time (Liu Yu etc., 2008) of area takes place over nearly 20 years.Therefore, carry out research in a deep going way, become the task of top priority in plant protection technology field rice leaf roller.
Studies all needs the consistent confession examination worm source of a large amount of development progresses, carries out the accuracy with result of study continuously with what guarantee research work.But, seriously restricted research to this worm biological property, evaluation of resistance and Prevention Technique etc. owing to lack a large amount of cnaphalocrocis snout moth's larva sources.And; Change along with cropping system; The confusion of rice growing phase causes that the abiogenesis of rice leaf roller field is irregular, generation overlap is serious, larva is unstable breeding time, simultaneously because a large amount of uses of changeable and the agricultural chemicals of weather cause that rice leaf roller field population is difficult to predict.Though existing many researchs about rice leaf roller artificial feed prescription and indoor a large amount of propagation techniques all do not obtain important breakthrough so far.For this reason, seeking a kind of method through artificial mass rearing rice leaf roller, to provide development progress consistent test worm source, is that the problem that solves is urgently hoped in the present technique field.
Summary of the invention
The objective of the invention is; Experiment worm source to traditional rice leaf roller mainly is collected in the nature field, is subject to the influence of abiogenesis situation of paddy growth state, rice leaf roller and the development progress of larva etc., causes worm source deficiency, worm source physiological status difference, defective such as the length of time is inconsistent and experimental result is inaccurate; A kind of method that is used for artificial mass rearing rice leaf roller is provided; Many to obtain quantity, the standardization of examination worm is realized in the length of time, the more consistent worm source of state.The object of the invention is achieved through following technical scheme.
The method of a large amount of continuously breeding rice leaf roller in a kind of field, this method is carried out according to the following steps:
(1), sets up the nylon solarium of 40m * 8m (long * wide) in the field; Be horizontally divided into 4 separate at least parts to the nylon solarium with nylon wire, sowed or transplanting in batches the hybrid rice that plant is tall and big, blade is roomy, tillering ability is strong in the part of independently cutting apart continuously in every separated 25-30 days by stages.In this step, hybrid rice can be excellent No. 1 of two line system or middle Zhejiang.
(2), normal field management, use more than or equal to 250kg/ha nitrogenous fertilizer, impel that paddy rice early sends out, excessive growth and light green, spray Buprofezin or the pyrrole aphid ketone control rice fulgorid invalid simultaneously to rice leaf roller;
(3), insert the rice leaf roller adult of gathering at first isolated part, utilize light green and the tall and big habit of becoming of rice leaf roller adult, concentrate and produce ovum in the tall and big paddy rice of sowing in advance regional from natural field in the rice tillering phase of containing; Along with the growth of rice leaf roller larvae, regularly gather different instar larvaes or pupa and be for experiment; The high instar larvae or the pupa that keep 10%-20% at last supply natural propagation in the first that isolates.
(4) contain the phase of sending out adult and open one of them second isolation nylon wire adjacent with first isolated part; Utilize light green habit of becoming of rice leaf roller adult and manual work to catch up with the moth method that the rice leaf roller adult is concentrated ovum once more and originate in second paddy rice zone, to keep the continuity of rice leaf roller population in the solarium.
Certainly, can also comprise that after step 4 repeating step 3 and 4 carries out in the next three isolation nylon wire adjacent with the second isolation nylon wire, continuing with rice leaf roller population in the maintenance solarium.
In addition, the nylon solarium is generally long 40 meters, and wide 8 meters, height are 4 meters circular arc type.Also have, the interval sowing here can be first independent isolating nylon wire and second independent isolating nylon wire time phase difference 25-30 days, and the sowing between second independent isolating nylon wire and the 3rd the independent isolating nylon wire differed 25-30 days; Sowing between the 3rd independent isolating nylon wire and the 4th the independent isolating nylon wire differed 25-30 days.Certainly; In selectable mode; 4 independently the seedling age between the paddy rice seedling of the transplanting between the independent isolating nylon wire differed 20-30 days; Guarantee rice leaf roller cycling deposition breeding continuously between four different independent nylon wires like this, can obtain larva not of the same age so throughout the year.
Like this; When the rice leaf roller that can work as first independent isolating nylon wire runs out of the nutrition that paddy rice provides; Let rice leaf roller get into next independent isolating nylon wire and proceed the breeding growth; Can carry out continuously like this, and then can get into the 3rd and the 4th independent isolating nylon wire and carry out cycling deposition and breeding, accomplish first cycling deposition.
Certainly, before getting into next circulation again, need remove, sow new paddy rice then and grow previous paddy rice in the isolation nylon wire of new entering.Sowing time can with the 4th independent isolating nylon wire in first circulation in differed 25-30 days or transplanting time 20-30 days at interval.
In the time of the larva of the some given age of needs; Can come to regulate arbitrarily the supply of the sowing time of paddy rice according to the production habit of leaf roll snout moth's larva with increase or minimizing nutrition; Let be in some moments, can satisfy the needs of different actual experiments like this in the specific period.
In a selectable mode; Can also comprise step 5, promptly during the adult Sheng was sent out, the cotton ball that 10% hydromel is arranged was inhaled in suspension all around in the nylon solarium; Provide the rice leaf roller adult to supplement the nutrients, improve the rice leaf roller population quantity to prolong the adult life-span, to increase egg laying amount.
Beneficial effect of the present invention:
(1) through the improvement of the present invention to the rice leaf roller rearing method; Not only realized the rice leaf roller field purpose of a large amount of propagating and breedings continuously simultaneously; Also can obtain each more consistent instar larvae such as the length of time, state; Overcome the worm source of in the past gathering from the abiogenesis rice field; The influence of the development progress that is subject to host plant growth conditions, rice leaf roller abiogenesis situation and larva that is brought etc. causes defectives such as worm source deficiency, worm source physiological status difference and experimental result are inaccurate, has guaranteed the standardization of experiment examination worm;
(2) in the method provided by the invention, can cover, regulate temperature, do sth. in advance or prolong the paddy rice implantation time through solarium's external application nylon membrane or nylon gauze.And the solarium is divided at least 4 parts, sowing by stages and in groups can guarantee that enough riotous growth, leaf look light green paddy rice confession rice leaf roller get food, does not allow to be subject to the influence of natural plantation situation of field water rice and weather etc.Like fund permission, the glass greenhouse of temperature control control light is reconstructed into by the solarium, then can not receive the influence of region, season, weather etc., can realize all-year raising, guarantee the needs of experiment.
Embodiment
Below the inventive method is done further to describe in detail, but should be appreciated that the present invention is not limited by these contents through embodiment.
Embodiment 1: the solarium makes up and the sub-district design
(1) sets up the nylon solarium (circular arc type, high 4 meters) of 8 * 40m in the field; Be horizontally divided into 4 parts to the nylon solarium with nylon wire, every separated 4 phases of 25-30 natural gift are sowed excellent No. 1 hybrid rice of two line system or middle Zhejiang that plant is tall and big, blade is roomy, tillering ability is strong respectively.
(2) normal field management, capacity is used nitrogenous fertilizer (more than the 250kg/ha), impels that paddy rice early sends out, excessive growth and light green, sprays Buprofezin or the pyrrole aphid ketone control rice fulgorid invalid to rice leaf roller.
(3) the rice tillering Sheng phase is inserted the rice leaf roller adult that gather in the field, utilizes the light green and tall and big habit of becoming of rice leaf roller adult, concentrates and produces ovum in the local tall and big paddy rice zone of sowing in advance.Along with the growth of rice leaf roller larvae, regularly gather different instar larvaes or pupa and be for experiment.The high instar larvae of the final 10%-20% of reservation or pupa supply natural propagation.
(4) contain the phase of sending out adult and open one of them adjacent isolation nylon wire; Utilize light green habit of becoming of rice leaf roller adult and the artificial method of moth of catching up with to make the concentrated paddy rice zone of originating in down the first phase sowing of its ovum once more, keep the continuity of rice leaf roller population in the solarium.
Result of the test shows, transplants in batches and can obviously improve the quantity of the rice leaf roller of acquisition, and on the basis of improving quantity, can guarantee to obtain worm relatively more consistent larva in age.As shown in table 1, divide three times and four times to transplant, each batch collect 3 age rice leaf roller larvae be significantly higher than and once transplant and twice transplanting.
Table 1 different disposal obtains the quantity (head/clump paddy rice) of rice leaf roller 3 instar larvaes
Embodiment 2: different planting is to raising the influence of rice leaf roller
With the solarium that embodiment 1 is built, relatively different water rice culture mode is to raising the effect of rice leaf roller.Planting type is little to the influence of raising rice leaf roller, and the quantity of the rice leaf roller that collects on the transplanting rice is a little more than direct seading rice, but does not have significant difference.
The rice leaf roller 3 instar larvae quantity of gathering in the crops under table 2 different planting (head/clump paddy rice)
Embodiment 3: different nitrogenous fertilizer are to raising the influence of rice leaf roller
With the solarium that embodiment 1 is built, study different nitrogenous fertilizer levels to raising the influence of rice leaf roller effect.Obtain quantity during during for 250kg/ha in the nitrogenous fertilizer usage amount apparently higher than 100kg/ha with rice leaf roller larvae in the length of time.
The different nitrogenous fertilizer usage amounts of table 3 (kg/ha) are the rice leaf roller 3 instar larvae quantity (head/clump paddy rice) of results down
Embodiment 4: adult supplements the nutrients to raising the influence of rice leaf roller
Results rice leaf roller 3 instar larvae quantity (head/clump paddy rice) under the different adult extra-nutrition of table 4 condition
With the solarium that embodiment 1 is built, the research adult supplements the nutrients to raising the influence of rice leaf roller effect.Behind the adult eclosion, suspension all around contains the cotton ball of 10% hydromel in the nylon solarium.The result shows that adult supplements the nutrients can obviously improve the feeding effect of rice leaf roller, but extra-nutrition is provided for a long time and only in the adult peak period no significant difference between the extra-nutrition is provided.
Claims (3)
1. methods of a large amount of continuously breeding rice leaf roller in a field is characterized in that this method may further comprise the steps:
(1), sets up long 40m in the field; The nylon solarium of wide 8m; Be horizontally divided into 4 separate at least parts to the nylon solarium with nylon wire, sowed or transplanting in batches the hybrid rice that plant is tall and big, blade is roomy, tillering ability is strong in the part of independently cutting apart continuously in every separated 25-30 days by stages;
(2), normal field management, use more than or equal to 250kg/ha nitrogenous fertilizer, impel that paddy rice early sends out, excessive growth and light green, spray Buprofezin or the pyrrole aphid ketone control rice fulgorid invalid simultaneously to rice leaf roller;
(3), insert the rice leaf roller adult of gathering at first isolated part, utilize light green and the tall and big habit of becoming of rice leaf roller adult, concentrate and produce ovum in the tall and big paddy rice of sowing in advance regional from natural field in the rice tillering phase of containing; Along with the growth of rice leaf roller larvae, regularly gather different instar larvaes or pupa and be for experiment; The high instar larvae or the pupa that keep 10%-20% at last supply natural propagation in the first that isolates;
(4), contain the phase of sending out adult and open one of them second isolation nylon wire adjacent with first isolated part; Utilize light green habit of becoming of rice leaf roller adult and manual work to catch up with the moth method that the rice leaf roller adult is concentrated ovum once more and originate in second paddy rice zone, to keep the continuity of rice leaf roller population in the solarium.
2. method according to claim 1 is characterized in that, this method comprises that repeating step 3 and 4 carries out continuing in the 3rd isolated part and the 4th isolated part with rice leaf roller population in the maintenance solarium.
3. according to the described method of one of claim 1-2; It is characterized in that; This method comprises: during the adult Sheng is sent out; The cotton ball that 10% hydromel is arranged is inhaled in suspension around in the nylon solarium, provides the rice leaf roller adult to supplement the nutrients to prolong the adult life-span, to increase egg laying amount, improves the rice leaf roller population quantity.
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| CN201010537023A CN101984799B (en) | 2010-11-09 | 2010-11-09 | Method for continuously propagating large amount of Cnaphalocrocis medinalis in field |
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| CN201010537023A CN101984799B (en) | 2010-11-09 | 2010-11-09 | Method for continuously propagating large amount of Cnaphalocrocis medinalis in field |
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| CN102578045B (en) * | 2012-02-15 | 2014-02-05 | 中国农业科学院植物保护研究所 | Method and special device for propagating insects |
| CN102845268A (en) * | 2012-09-10 | 2013-01-02 | 湖南省水稻研究所 | Integrated rice production method based on wasps, frogs and lights |
| CN103782966B (en) * | 2014-02-13 | 2015-06-10 | 云南省农业科学院生物技术与种质资源研究所 | Method for identifying rice planthopper resistance of rice |
| CN106212386A (en) * | 2016-07-18 | 2016-12-14 | 青海省农林科学院 | A kind of field sizeization raises the method for Fructus Foeniculi thin wing open country snout moth's larva |
| CN112034097B (en) * | 2019-07-31 | 2022-06-07 | 广西壮族自治区植保站 | Method for determining trapping and killing effect of prodenia litura adults |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101796939A (en) * | 2010-02-25 | 2010-08-11 | 浙江省农业科学院 | Egg laying device of cnaphalocrocis medinalis guenee and use method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101796939A (en) * | 2010-02-25 | 2010-08-11 | 浙江省农业科学院 | Egg laying device of cnaphalocrocis medinalis guenee and use method thereof |
Non-Patent Citations (3)
| Title |
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| 张俊平等.稻纵卷叶螟种群系统动态模拟.《昆虫学报》.1991,第34卷(第3期),311－318. * |
| 顾海南等.自然条件下稻纵卷叶螟的繁殖特性.《南京农业大学学报》.1987,(第4期),37-41. * |
| 齐国君等.安徽安庆混作稻区稻纵卷叶螟第三、四代发生规律研究.《中国水稻科学》.2008,第22卷(第5期),513－518. * |
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