CN103782966B - Method for identifying rice planthopper resistance of rice - Google Patents
Method for identifying rice planthopper resistance of rice Download PDFInfo
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- CN103782966B CN103782966B CN201410049067.4A CN201410049067A CN103782966B CN 103782966 B CN103782966 B CN 103782966B CN 201410049067 A CN201410049067 A CN 201410049067A CN 103782966 B CN103782966 B CN 103782966B
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Abstract
The invention discloses a method for identifying the rice planthopper resistance of rice. A device for identifying the rice planthopper resistance of the rice is used for carrying out identification on the rice planthopper resistance of the rice, collected living rice planthoppers are taken into a rice planthopper source cultivating room to carry out purification cultivation, when to-be-identified rice materials cultivated in rice identification material cultivating rooms are in the quick tillering season, valves are opened, the rice planthoppers migrate into the rice identification material cultivating rooms through natural transmission to parasitize on the to-be-identified rice materials, after 71 hours to 72 hours, the valves are closed, and insect resistance identification is carried out on the to-be-identified rice materials according to the phenotypic characteristics when all the rice planthopper high-infected contrast materials are dead. According to the method, influences of external conditions on growth of the rice planthoppers are ingeniously avoided, the complex process of preparing a plurality of nymphs or adults is omitted in the inoculation conditions, limitation of rice planthopper stages is avoided during inoculation, inevitable damage of manual rice planthopper transferring to the rice planthoppers is avoided, meanwhile, the condition that different rice planthoppers are mixed to enter the same identification materials in one greenhouse is also avoided, and the data accuracy after inoculation is guaranteed.
Description
Technical field
The present invention relates to a kind of Rice Resistance rice fulgorid authentication method, belong to crops and insect pest makes studying technological domain mutually.
Technical background
In order to ensure the food of sustainable growth population, it is extremely important for controlling rice insect pest.A large amount of scientific researches and production practices prove, in the comprehensive regulation of rice grub, cultivating and promote pest-resistant cultivar can effectively Control pests population, Protect natural enemies, the using and reduce production cost of minimizing insecticide, and can reach the object of Initiative defence.
Rice fulgorid is the important pests in China and many Asian countries Rice Production, to sting crops such as inhaling plant juice hazard rice, often causes the large stretch of dead stalk lodging of paddy rice, the significantly underproduction.At present, rice fulgorid harm is day by day serious, and the hereditary basis of cultivated rice against-plant hopper is narrow, and need to be excavated the anti-source material of new against-plant hopper, therefore, the qualification of the rice material of against-plant hopper is particularly important.
Up to the present, according to present bibliographical information, evaluation rice makes resource against-plant hopper method and sets up, adopt seedling stage colony's Artificial Inoculation of Anoplophora glabripennis, seedling stage individual plant connect worm qualification and tillering regularity individual plant connect worm authentication method and Natural infection method, the resistant phenotype data obtained in these methods are Natural infection the most reliably.Seedling stage colony's Artificial Inoculation of Anoplophora glabripennis, seedling stage individual plant connect worm qualification and tillering regularity individual plant and connect worm authentication method three kinds to connect worm method workload extremely large, especially be difficult to ensure to have enough inoculation worm sources at short notice, also the difficult unified worm source connect is all the rice fulgorid nymph in 2-3 age.To measure 30 resistance to insectss for examination material, 3 repetitions are set up in experiment, each material has 10 strains, every strain at least needs 10 nymphs, preparation 9000 nymphs are just needed when doing this experiment, if these experiment materials will be obtained very accurately, need the larger work energy of cost and experience the very loaded down with trivial details course of work.Although Natural infection method is reliable, but Natural infection timeliness is too strong, rice fulgorid belongs to migratory pest, can cause harm with wind and rain breeding of migrating at a distance, also there is fulminant and sudden, namely need to carry out rice fulgorid insect pest evaluation in the rice fulgorid emergence period, also easily affect by unfavorable factors such as region, weather and worm sources.
These difficult problems are badly in need of wanting feasible solution, if Natural infection can be simulated in laboratory or greenhouse to the qualification work carrying out against-plant hopper for examination material, this will increase work efficiency greatly, and scientific data can be obtained exactly, for the resistance screening of seed rice resistance to insects material provides strong foundation.
Summary of the invention
Preparing that inoculation rice fulgorid worm amount is difficult to ensure, Natural infection timeliness is strong for overcoming in prior art, by defect and deficiencies such as the restrictions of the factor such as region, weather, the invention provides and a kind of authentication method that conveniently can provide rice fulgorid worm source, reduce workload, can obtain the Rice Resistance rice fulgorid of appraising datum accurately and reliably is simultaneously provided.
The authentication method of Rice Resistance rice fulgorid provided by the present invention, adopts Rice Resistance rice fulgorid identification apparatus to carry out the qualification of Rice Resistance rice fulgorid, comprises the following steps:
A. accessed in rice fulgorid Chong Yuan culturing room by the live body rice fulgorid gathered from field, purifying is cultivated, now all valve closings leading to paddy rice expert evidence plantation room;
B. plant the control material of indoor plantation rice material to be identified and high sense rice fulgorid at paddy rice expert evidence, wait rice material to be identified to be in tillering regularity, open valve; Rice fulgorid moves to paddy rice expert evidence plantation indoor by natural propagation, and parasitizes rice material to be identified;
C.71 ~ 72h, valve-off, stops the propagation of rice fulgorid;
D., when the control material of height sense rice fulgorid is all dead, according to phenotypic characteristic, insect resistance identification is carried out to rice material to be identified;
Described Rice Resistance rice fulgorid identification apparatus, be provided with rice fulgorid Chong Yuan culturing room and at least one paddy rice expert evidence plantation room, the identical structure that described rice fulgorid Chong Yuan culturing room and paddy rice expert evidence plant room is: on the wall of cylinder, be fixedly connected with at least three vertical bars, the upper end of cylinder is fixedly connected with doorframe, the top of doorframe is fixedly connected with a beam, the two ends of door beam are fixedly connected with adjacent vertical bar respectively, doorframe is provided with can the door of switch, door is provided with keeper, the top of vertical bar is fixedly connected with annulus I, in the annular groove of the bottom inserted base of cylinder and with annular groove matched in clearance, the edge of base is fixedly connected with fixed block, fixed block is provided with fixing hole, the aperture of pore ring I is equal with the aperture of pore ring II and equal with the aperture of rice fulgorid passage, the outside of the vertical bar of the outside of the vertical bar of rice fulgorid Chong Yuan culturing room and paddy rice expert evidence plantation room is provided with guard respectively, the end mouth of guard is connected with the outer wall of cylinder, guard having the hole identical with the shape of the shape of the shape of corresponding pore ring I, pore ring II, doorframe with pore ring I or pore ring II and position corresponding to doorframe, the edge in each hole is fixedly connected with corresponding pore ring I, pore ring II, doorframe respectively, the aperture of described guard is 0.1mm ~ 0.3mm,
Described rice fulgorid Chong Yuan culturing room from the structure that paddy rice expert evidence plants room different is: be fixedly connected with annulus II and annulus III at the middle part of the vertical bar of rice fulgorid Chong Yuan culturing room, be fixedly connected with pore ring I between annulus II and annulus III, it is equal that quantity and the paddy rice expert evidence of pore ring I plant number of chambers amount; Annulus IV and annulus V is fixedly connected with on the top of the vertical vertical rod of paddy rice expert evidence plantation room, a pore ring II is fixedly connected with between annulus IV and annulus V, on the vertical bar of rice fulgorid Chong Yuan culturing room, the position of annulus II and annulus III is all lower than the annulus IV on the vertical bar of paddy rice expert evidence plantation room and annulus V, and pore ring I is lower than pore ring II;
The annexation of described rice fulgorid Chong Yuan culturing room and paddy rice expert evidence plantation room is: rice fulgorid Chong Yuan culturing room and the paddy rice expert evidence plantation room rice fulgorid expanding channels by tilting, the pore ring II that the two ends of rice fulgorid passage are planted on room with the pore ring I in rice fulgorid Chong Yuan culturing room and paddy rice expert evidence is respectively fixedly connected with, and described rice fulgorid passage is provided with valve.
The center line of described rice fulgorid passage and the angle α of horizontal plane are 45 ° ~ 60 °.
Paddy rice expert evidence in replaceable paddy rice expert evidence plantation room 2, carries out identical operation according to above-mentioned steps A-D, other rice material is carried out to the qualification of Rice Resistance rice fulgorid.
The invention has the beneficial effects as follows, solve the impact that external condition grows rice fulgorid dexterously, make the life condition of rice fulgorid can be optimized to the pattern of rice fulgorid outbreak period, inoculation condition eliminates the complicated processes preparing some nymphs or adult, not by the restriction of worm phase when inoculation rice fulgorid, paddy rice expert evidence plantation room can be planted at any time by needing the seed rice material of qualification, rice fulgorid Chong Yuan culturing room and paddy rice expert evidence plant connection between room by rice fulgorid expanding channels, because the horizontal inclination alpha of this rice fulgorid passage is tilting, namely rice fulgorid passage and paddy rice expert evidence plant port that room is connected higher than the port be connected with rice fulgorid Chong Yuan culturing room with rice fulgorid passage, utilize the upper property that becomes of rice fulgorid, when empirically plan need be identified rice material to be identified, only need open the process that valve just can complete simulating natural condition, rely on rice fulgorid natural propagation, to the inevitable injury that worm is caused when avoiding manually turning worm, it also avoid rice fulgorid in operation to fly out situation about propagating separately, also overcome different rice fulgorid in a greenhouse simultaneously and mutually mix the situation entering same expert evidence, ensure that the accuracy of the rear data of inoculation.Change rice strain or reseed seed rice simple.
The described device that the present invention adopts can be planted room with several corresponding paddy rice expert evidences in rice fulgorid Chong Yuan culturing room respectively by several rice fulgorid passages and be connected, therefore, the Resistance Identification of against-plant hopper can be carried out simultaneously to multiple rice material to be identified simultaneously, save scientific research time, workload and cost in large quantities.
Described device also can be installed easily in field, utilize the present invention to carry out Resistance Identification, efficiently avoid the interference of other sick worms, make experimental data have more reliability; In greenhouse, utilize the present invention, can multiple described device be utilized to cultivate the rice fulgorid of some geographical population in a greenhouse simultaneously, greatly can save greenhouse space like this, and effectively utilize the energy.
The present invention also can as cultivating the cultural method that infection insect live body is preserved, virus is preserved, and the operating process that preservation insect vector transmitted virus is preserved simplifies and accurately, avoids because infection insect effusion causes cross pollution.
Accompanying drawing explanation
Fig. 1 is the structural representation of the Rice Resistance rice fulgorid identification apparatus that the present invention adopts.
Fig. 2 is the structural representation not arranging guard in Fig. 1.
Fig. 3 is the structural representation of rice fulgorid Chong Yuan culturing room in Fig. 2.
Fig. 4 is the structural representation of paddy rice expert evidence plantation room in Fig. 2.
Fig. 5 is the structural representation of base.
In figure, each mark represents successively:
1-rice fulgorid Chong Yuan culturing room; 2-paddy rice expert evidence plantation room; 3-base; 4-rice fulgorid passage; 5-valve; 6-guard; 7-vertically bar; 8-annulus, I: 9-annulus II; 10-annulus III; 11-cylinder; 12-annulus IV; 13-annulus, V: 14-pore ring I; 15-door beam; 16-doorframe; 17-door; 18-keeper; 19-pore ring II; 20-fixed block: 21-fixing hole; 22-annular groove; α is the angle of rice fulgorid channel centerline and horizontal plane.
Embodiment
Be conventional method if no special instructions in following embodiment.
The authentication method of Rice Resistance rice fulgorid provided by the present invention, adopts Rice Resistance rice fulgorid identification apparatus to carry out the qualification of Rice Resistance rice fulgorid, comprises the following steps:
A. accessed in rice fulgorid Chong Yuan culturing room 1 by the live body rice fulgorid gathered from field, purifying is cultivated, and now all valves 5 leading to paddy rice expert evidence plantation room 2 are closed;
B. the rice material that plantation is to be identified in paddy rice expert evidence plantation room 2 and the control material of high sense rice fulgorid, wait rice material to be identified to be in tillering regularity, open valve 5; Rice fulgorid is moved in paddy rice expert evidence plantation room 2 by natural propagation, and parasitizes rice material to be identified;
C.71h or 72h, valve-off 5, stops the propagation of rice fulgorid;
D., when the control material of height sense rice fulgorid is all dead, according to phenotypic characteristic, insect resistance identification is carried out to rice material to be identified.
Rice material to be identified also in replaceable paddy rice expert evidence plantation room 2, carries out identical operation according to above-mentioned steps A-D, other rice material is carried out to the qualification of Rice Resistance rice fulgorid.
Composition graphs 1-Fig. 5, Rice Resistance rice fulgorid identification apparatus provided by the present invention, be provided with rice fulgorid Chong Yuan culturing room 1 and at least one paddy rice expert evidence plantation room 2, the identical structure that described rice fulgorid Chong Yuan culturing room 1 and paddy rice expert evidence plant room 2 is: on the wall of cylinder 11, be fixedly connected with at least three vertical bar 7(cylinders i.e. its top and bottom open, the perisporium of vertical direction is closed), the upper end of cylinder 11 is fixedly connected with doorframe 16, the top of doorframe 16 is fixedly connected with a beam 15, the two ends of door beam 15 are fixedly connected with adjacent vertical bar 7 respectively, doorframe 16 is provided with can the door 17 of switch, the side of side and doorframe 16 that door 17 is provided with keeper 18(door 17 is connected through the hinge, the opposite side of door 17 connects with crossing keeper 18 to the corresponding side of doorframe 16), the top of vertical bar 7 is fixedly connected with annulus I 8, in the annular groove 22 of the bottom inserted base 3 of cylinder 11 and with annular groove 22 matched in clearance, the edge of base 3 is fixedly connected with fixed block 20, fixed block 20 is provided with fixing hole 21, base 3 is connected by the fixing hole 21 of bolt through fixed block 20 with ground, or use other fixed forms, as long as the gravity according to base in greenhouse also can be put surely on ground.The aperture of pore ring I 14 is equal with the aperture of pore ring II 19 and equal with the aperture of rice fulgorid passage 4, the outside of the vertical bar 7 of the outside of the vertical bar 7 of rice fulgorid Chong Yuan culturing room and paddy rice expert evidence plantation room 2 is provided with guard 6 respectively, and the end mouth of guard 6 is connected with the outer wall of cylinder 11; Guard having the hole identical with the shape of the shape of the shape of corresponding pore ring I 14, pore ring II 19, doorframe 16 with pore ring I 14 or pore ring II 19 and position corresponding to doorframe 16, the edge in each hole is fixedly connected with corresponding pore ring I 14, pore ring II 19 or doorframe 16 respectively, the aperture of described guard 6 is 0.2mm;
Described rice fulgorid Chong Yuan culturing room 1 from the structure that paddy rice expert evidence plants room 2 different is: be fixedly connected with annulus II 9 and annulus III 10 at the middle part of the vertical bar 7 of rice fulgorid Chong Yuan culturing room 1, be fixedly connected with pore ring I 14 between annulus II 9 and annulus III 10, it is equal that quantity and the paddy rice expert evidence of pore ring I 14 plant room 2 quantity; Annulus IV 12 and annulus V 13 is fixedly connected with on the top of the vertical vertical rod 7 of paddy rice expert evidence plantation room 2, a pore ring II 19 is fixedly connected with between annulus IV 12 and annulus V 13, annulus II 9 on the vertical bar 7 of rice fulgorid Chong Yuan culturing room 1 and the position of annulus III 10 are all lower than the annulus IV 12 on the vertical bar 7 of paddy rice expert evidence plantation room 2 and annulus V 13, and pore ring I 14 is lower than pore ring II 19;
Rice fulgorid Chong Yuan culturing room 1 with the annexation of paddy rice expert evidence plantation room 2 is: rice fulgorid Chong Yuan culturing room 1 is connected by the rice fulgorid passage 4 tilted with paddy rice expert evidence plantation room 2, the pore ring II 19 that the two ends of rice fulgorid passage 4 are planted on room 2 with the pore ring I 14 in rice fulgorid Chong Yuan culturing room 1 and paddy rice expert evidence is respectively fixedly connected with, namely the inclination of its rice fulgorid passage 4 is one end that one end that rice fulgorid passage is connected with the pore ring I 14 in rice fulgorid Chong Yuan culturing room is connected lower than the pore ring II 19 that rice fulgorid passage and paddy rice expert evidence are planted on room 2, its angle of inclination is: the center line of rice fulgorid passage 4 and the angle α of horizontal plane are 45 ° or 60 °, rice fulgorid passage 4 is provided with valve 5.
Claims (2)
1. the authentication method of Rice Resistance rice fulgorid, is characterized in that, adopts Rice Resistance rice fulgorid identification apparatus to carry out the qualification of Rice Resistance rice fulgorid, comprises the following steps:
A. access in rice fulgorid Chong Yuan culturing room (1) by the live body rice fulgorid gathered from field, purifying is cultivated, and now all valves (5) leading to paddy rice expert evidence plantation room (2) are closed;
The control material of the rice material that B. plantation is to be identified in paddy rice expert evidence plantation room (2) and high sense rice fulgorid, waits rice material to be identified to be in tillering regularity, opens valve (5);
C.71 ~ 72h, valve-off (5), stops the propagation of rice fulgorid;
D., when the control material of height sense rice fulgorid is all dead, according to phenotypic characteristic, insect resistance identification is carried out to rice material to be identified;
Described Rice Resistance rice fulgorid identification apparatus, be provided with rice fulgorid Chong Yuan culturing room (1) and at least one paddy rice expert evidence plantation room (2), the identical structure that described rice fulgorid Chong Yuan culturing room (1) and paddy rice expert evidence plant room (2) is: on the wall of cylinder (11), be fixedly connected with at least three vertical bars (7), the upper end of cylinder (11) is fixedly connected with doorframe (16), the top of doorframe (16) is fixedly connected with a beam (15), the two ends of door beam (15) are fixedly connected with adjacent vertical bar (7) respectively, doorframe (16) is provided with can the door (17) of switch, door (17) is provided with keeper (18), the top of vertical bar (7) is fixedly connected with annulus I (8), in the annular groove (22) of cylinder (11) inserted base (3) and with annular groove (22) matched in clearance, the edge of base (3) is fixedly connected with fixed block (20), fixed block (20) is provided with fixing hole (21), the aperture phase of pore ring I (14) is equal with the aperture of pore ring II (19) and equal with the aperture of rice fulgorid passage (4), the outside of the vertical bar (7) of the outside of the vertical bar (7) of rice fulgorid Chong Yuan culturing room (1) and paddy rice expert evidence plantation room (2) is provided with guard (6) respectively, the end mouth of guard (6) is connected with the outer wall of cylinder (11), guard (6) having the hole identical with the shape of the shape of the shape of corresponding pore ring I (14), pore ring II (19), doorframe (16) with pore ring I (14) or pore ring II (19) and position corresponding to doorframe (16), the edge in each hole is fixedly connected with corresponding pore ring I (14), pore ring II (19), doorframe (16) respectively, the aperture of described guard (6) is 0.1mm ~ 0.3mm,
The structure that described rice fulgorid Chong Yuan culturing room (1) and paddy rice expert evidence plant room (2) different is: be fixedly connected with annulus II (9) and annulus III (10) at the middle part of the vertical bar (7) of rice fulgorid Chong Yuan culturing room (1), be fixedly connected with pore ring I (14) between annulus II (9) and annulus III (10), it is equal that quantity and the paddy rice expert evidence of pore ring I (14) plant room (2) quantity, annulus IV (12) and annulus V (13) is fixedly connected with on the top of the vertical bar (7) of paddy rice expert evidence plantation room (2), a pore ring II (19) is fixedly connected with between annulus IV (12) and annulus V (13), the upper annulus II (9) of vertical bar (7) of rice fulgorid Chong Yuan culturing room (1) and the position of annulus III (10) are all lower than the annulus IV (12) on the vertical bar (7) of paddy rice expert evidence plantation room (2) and annulus V (13), pore ring I (14) is lower than pore ring II (19),
Described rice fulgorid Chong Yuan culturing room (1) with the annexation of paddy rice expert evidence plantation room (2) is: the pore ring II (19) that the two ends of rice fulgorid passage (4) are planted on room (2) with the pore ring I (14) in rice fulgorid Chong Yuan culturing room (1) and paddy rice expert evidence is respectively fixedly connected with, and rice fulgorid passage (4) is provided with valve (5).
2. the authentication method of Rice Resistance rice fulgorid according to claim 1, is characterized in that: the center line of rice fulgorid passage (4) of described Rice Resistance rice fulgorid identification apparatus and the angle α of horizontal plane are 45 ° ~ 60 °.
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| CN201410049067.4A CN103782966B (en) | 2014-02-13 | 2014-02-13 | Method for identifying rice planthopper resistance of rice |
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| CN201410049067.4A CN103782966B (en) | 2014-02-13 | 2014-02-13 | Method for identifying rice planthopper resistance of rice |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105165502B (en) * | 2015-07-21 | 2017-11-07 | 中国水稻研究所 | A kind of method of half field evaluation paddy rice Adult plant whitebacked planthopper-resistance |
| CN108184578B (en) * | 2017-12-22 | 2021-01-05 | 武汉禾泰青生物科技有限公司 | Identification method for brown planthopper resistance of rice |
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| CN101822187A (en) * | 2010-04-30 | 2010-09-08 | 浙江省农业科学院 | Method for evaluating rice planthopper-resistant property of rice |
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| CN202222293U (en) * | 2011-08-09 | 2012-05-23 | 浙江省农业科学院 | Breeding device for rice planthopper, leafhopper and leaf roller |
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| KR101312680B1 (en) * | 2011-10-12 | 2013-09-27 | 경기도 | Ecology experience kits for photo-tactic insects |
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| CN101822187A (en) * | 2010-04-30 | 2010-09-08 | 浙江省农业科学院 | Method for evaluating rice planthopper-resistant property of rice |
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| CN202222293U (en) * | 2011-08-09 | 2012-05-23 | 浙江省农业科学院 | Breeding device for rice planthopper, leafhopper and leaf roller |
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