CN103766202A - Dendrobium officinale tissue culture seedling hardening method - Google Patents
Dendrobium officinale tissue culture seedling hardening method Download PDFInfo
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- CN103766202A CN103766202A CN201310724081.5A CN201310724081A CN103766202A CN 103766202 A CN103766202 A CN 103766202A CN 201310724081 A CN201310724081 A CN 201310724081A CN 103766202 A CN103766202 A CN 103766202A
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Abstract
The invention relates to the technical field of plant cultivation, in particular to a dendrobium officinale tissue culture seedling hardening method. The dendrobium officinale tissue culture seedling hardening method includes the steps of conducting bottle seedling selection on tissue culture seedlings, selecting seedling hardening matrixes, managing bottle seedlings before the bottle seedlings are taken out of a bottle, processing the bottle seedlings after the bottle seedlings are taken out of the bottle, and conducting management after transplanting. According to the dendrobium officinale tissue culture seedling hardening method, the dendrobium officinale tissue culture seedlings after seedling hardening are healthy and strong, the leaf color is deep green, root systems are flourished, and the survival rate of transplanting is greatly improved.
Description
Technical field
The present invention relates to field of plant growing technology, more specifically, relate to a kind of hardening off method of candidum tissue culturing seedling.
Background technology
Dendrobium candidum is the essential raw material of domestic and international nearly hundred kinds of medicines and health products at present, the whole nation relates to the pharmaceutical factory and the health products processing enterprise that use stem of noble dendrobium class family more than 100, China every year medicine take dendrobium candidum as raw material, the health products output value exceedes hundred million yuan of hundreds ofs, the quantity of the market demand is often every year more than millions of kilograms, and in rising trend year by year, due to the rush of demand of market to dendrobium candidum medicinal material.
Current, dendrobium candidum batch production is produced and is mainly expanded numerously by cultivation group training seedling, and the group training seedling autophyting ability of just having produced from group training chamber is very poor, and the photosynthesis of dredging function and blade of absorption function, fleshy stem that is mainly manifested in root is poor; Adaptability is to external world not strong, causes transplanting survival rate not high.Before facility or field cultivation, must pass through this important step of hardening from group training chamber, traditional hardening off method normally bottle seedling first adapts to a period of time (general 5-10 days) through indoor environment, open gradually bottle cap and carry out transition (3-5 days), and then bottle outlet, wash medium off, the transition hardening before giving high humidity (90% above humidity), low light level processing and normally cultivating.But by traditional Trained Transition Methods of Chinese Dwarf, the survival rate of stem of noble dendrobium class group training seedling or undesirable, makes batch production production seedling cost maintain higher level.Tradition hardening off method does not have to solve the problem that absorption function is poor, plant is fragile, adaptability is not strong of stem of noble dendrobium class group training shoot root system at all.Therefore, traditional hardening off method cannot guarantee the transplanting survival rate of stem of noble dendrobium class group training seedling, causes group training shoot root system and stem section is rotten, lethality is higher.
Summary of the invention
The present invention is at least one defect overcoming described in above-mentioned prior art, and a kind of hardening off method of candidum tissue culturing seedling is provided, and this hardening off method makes the candidum tissue culturing seedling stalwartness after hardening, and leaf look dark green, well developed root system, and transplanting survival rate improves greatly.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
A kind of hardening off method of candidum tissue culturing seedling is provided, and its step comprises:
(1) group training seedling bottle seedling is selected: individual plant inoculation group training seedling plant height reaches more than 3 centimetres, has 2 above root systems,
(2) selection of hardening matrix, the matrix of hardening is sphagna, the fertilizer of hardening is the organic manure that herbivore manure fermentation makes,
(3) management before bottle seedling bottle outlet: group training seedling goes out group training chamber and is placed on spray chamber seedbed, upper covering nonwoven prevents from polluting, hardening temperature is controlled at 5 ℃-35 ℃, intensity of illumination is controlled at: first 7 days intensities of illumination are that 1500-3000lux, second 7 days intensity of illumination are 3000-8000lux, to stablize controlled light intensity be afterwards 8000-15000lux
(4) bottle outlet post processing: adopting long not wide ancient height is the square position of 54cm*27cm*6cm, take sphagna as hardening matrix, sphagna is soaked in water 12 hours, spin-dry, the weigh sphagna of 85 grams of every dish, manually pulls after loose and is filled to square position, the hardening matrix that the about 4cm of height of formation is high; Wash seedling: seedling is taken out gently from bottle, in clear water, the medium adhering to is cleaned up, seedling is made thinner, dried in the shade, after about more than 2 hours, turn white to root system; Transplant: adopt mass fraction carbendazim solution O.05% to dip in root 2-3 second, 3 one clump of strain, every clump of interval 5cm transplants to square position,
(5) easily carry rear management: the indoor humidity of spray chamber is maintained more than 90%, and illumination is controlled at 1500-3000lux; After 5-7 days, controlled humidity is 80-85%, and illumination is controlled at 3000-8000lux; After 5-7 days, adopt humidity 75-80%, illumination 8000-15000lux; After 30 days, can transplant to land for growing field crops.
One is preferably: described controlled light intensity is used two layers of removable shade net.
Compared with prior art, the invention has the beneficial effects as follows:
The candidum tissue culturing seedling that the present invention cultivates by hardening technology, through the program of drying in the shade in bottle outlet post processing, can greatly promote the generation of the aerial root of seedling, root system rotting rate obviously reduces, shoot survival percent reaches 95.7%, be significantly higher than existing 82.5% survival rate, and seedling stalwartness, leaf look dark green, well developed root system.
The present invention adopts hardening matrix and organic manure to mutually combine by the dendrobium candidum hardening off method providing, and can reduce hardening cost, simplifies hardening operation, and gained matrix has the loose feature of breathing freely, can keep moisture and nutrient; Can be dendrobium candidum adequate nutrients be provided, in hardening process without adding chemical fertilizer and nutrient solution.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment 1
A kind of hardening off method of candidum tissue culturing seedling is provided, and its step comprises:
(1) group training seedling bottle seedling is selected: individual plant inoculation group training seedling plant height reaches more than 3 centimetres, has 2 above root systems,
(2) selection of hardening matrix, the matrix of hardening is sphagna, the fertilizer of hardening is the organic manure that herbivore manure fermentation makes,
(3) management before bottle seedling bottle outlet: group training seedling goes out group training chamber and is placed on spray chamber seedbed, upper covering nonwoven prevents from polluting, hardening temperature is controlled at 5 ℃, intensity of illumination is controlled at: first 7 days intensities of illumination are that 3000lux, second 7 days intensity of illumination are 8000lux, to stablize controlled light intensity be afterwards 15000lux, and described controlled light intensity is used two layers of removable shade net.Two layers of above-mentioned removable shade net are selectively applied according to actual light intensity.Bottle cap 30 day time of full envelope, more loose lid 3 days, then take off and cover 2 days.
(4) bottle outlet post processing: adopting long not wide ancient height is the square position of 54cm*27cm*6cm, take sphagna as hardening matrix, sphagna is soaked in water 12 hours, spin-dry, the weigh sphagna of 85 grams of every dish, manually pulls after loose and is filled to square position, the hardening matrix that the about 4cm of height of formation is high; Wash seedling: seedling is taken out gently from bottle, in clear water, the medium adhering to is cleaned up, seedling is made thinner, dried in the shade, after about more than 2 hours, turn white to root system; Transplant: adopt mass fraction carbendazim solution O.05% to dip in for 3 seconds, 3 one clump of strain, every clump of interval 5cm transplants to square position,
(5) easily carry rear management: the indoor humidity of spray chamber is maintained more than 90%, and illumination is controlled at 3000lux; After 7 days, controlled humidity is 85%, and illumination is controlled at 8000lux; After 7 days, adopt humidity 80%, illumination 15000lux; After 30 days, can transplant to land for growing field crops.
Embodiment 2
A hardening off method for candidum tissue culturing seedling, its step comprises:
(1) group training seedling bottle seedling is selected: individual plant inoculation group training seedling plant height reaches more than 3 centimetres, has 2 above root systems,
(2) selection of hardening matrix, the matrix of hardening is sphagna, the fertilizer of hardening is the organic manure that herbivore manure fermentation makes,
(3) management before bottle seedling bottle outlet: group training seedling goes out group training chamber and is placed on spray chamber seedbed, upper covering nonwoven prevents from polluting, hardening temperature is controlled at 35 ℃, intensity of illumination is controlled at: first 7 days intensities of illumination are that 1500lux, second 7 days intensity of illumination are 3000lux, to stablize controlled light intensity be afterwards 8000lux, and described controlled light intensity is used two layers of removable shade net.Two layers of above-mentioned removable shade net are selectively applied according to actual light intensity.Bottle cap 30 day time of full envelope, more loose lid 3 days, then take off and cover 2 days.
(4) bottle outlet post processing: adopting long not wide ancient height is the square position of 54cm*27cm*6cm, take sphagna as hardening matrix, sphagna is soaked in water 12 hours, spin-dry, the weigh sphagna of 85 grams of every dish, manually pulls after loose and is filled to square position, the hardening matrix that the about 4cm of height of formation is high; Wash seedling: seedling is taken out gently from bottle, in clear water, the medium adhering to is cleaned up, seedling is made thinner, dried in the shade, after about more than 2 hours, turn white to root system; Transplant: adopt carbendazim solution O.05% of mass fraction to dip in 2 seconds of root, 3 one clump of strain, every clump of interval 5cm transplants to square position,
(5) easily carry rear management: the indoor humidity of spray chamber is maintained more than 90%, and illumination is controlled at 1500lux; After 5-7 days, controlled humidity is 80%, and illumination is controlled at 3000lux; After 5 days, adopt humidity 75%, illumination 8000lux; After 30 days, can transplant to land for growing field crops.
Embodiment 3
A hardening off method for candidum tissue culturing seedling, its step comprises:
(1) group training seedling bottle seedling is selected: individual plant inoculation group training seedling plant height reaches more than 3 centimetres, has 2 above root systems,
(2) selection of hardening matrix, the matrix of hardening is sphagna, the fertilizer of hardening is the organic manure that herbivore manure fermentation makes,
(3) management before bottle seedling bottle outlet: group training seedling goes out group training chamber and is placed on spray chamber seedbed, upper covering nonwoven prevents from polluting, hardening temperature is controlled at 25 ℃, intensity of illumination is controlled at: first 7 days intensities of illumination are that 2100lux, second 7 days intensity of illumination are 5100lux, to stablize controlled light intensity be afterwards 11000ux
(4) bottle outlet post processing: adopting long not wide ancient height is the square position of 54cm*27cm*6cm, take sphagna as hardening matrix, sphagna is soaked in water 12 hours, spin-dry, the weigh sphagna of 85 grams of every dish, manually pulls after loose and is filled to square position, the hardening matrix that the about 4cm of height of formation is high; Wash seedling: seedling is taken out gently from bottle, in clear water, the medium adhering to is cleaned up, seedling is made thinner, dried in the shade, after about more than 2 hours, turn white to root system; Transplant: adopt mass fraction carbendazim solution O.05% to dip in root 2-3 second, 3 one clump of strain, every clump of interval 5cm transplants to square position,
(5) easily carry rear management: the indoor humidity of spray chamber is maintained more than 90%, and illumination is controlled at 2200lux; After 6 days, controlled humidity is 83%, and illumination is controlled at 5600lux; After 6 days, adopt humidity 78%, illumination 11000lux; After 30 days, can transplant to land for growing field crops.
Obviously, the above embodiment of the present invention is only for example of the present invention is clearly described, and is not the restriction to embodiments of the present invention.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here without also giving exhaustive to all embodiments.All any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in the protection domain of the claims in the present invention.
Claims (2)
1. a hardening off method for candidum tissue culturing seedling, is characterized in that, its step comprises:
(1) group training seedling bottle seedling is selected: individual plant inoculation group training seedling plant height reaches more than 3 centimetres, has 2 above root systems,
(2) selection of hardening matrix, the matrix of hardening is sphagna, the fertilizer of hardening is the organic manure that herbivore manure fermentation makes,
(3) management before bottle seedling bottle outlet: group training seedling goes out group training chamber and is placed on spray chamber seedbed, upper covering nonwoven prevents from polluting, hardening temperature is controlled at 5 ℃-35 ℃, intensity of illumination is controlled at: first 7 days intensities of illumination are that 1500-3000lux, second 7 days intensity of illumination are 3000-8000lux, to stablize controlled light intensity be afterwards 8000-15000lux
(4) bottle outlet post processing: adopting long not wide ancient height is the square position of 54cm*27cm*6cm, take sphagna as hardening matrix, sphagna is soaked in water 12 hours, spin-dry, the weigh sphagna of 85 grams of every dish, manually pulls after loose and is filled to square position, the hardening matrix that the about 4cm of height of formation is high; Wash seedling: seedling is taken out gently from bottle, in clear water, the medium adhering to is cleaned up, seedling is made thinner, dried in the shade, after about more than 2 hours, turn white to root system; Transplant: adopt mass fraction carbendazim solution O.05% to dip in root 2-3 second, 3 one clump of strain, every clump of interval 5cm transplants to square position,
(5) easily carry rear management: the indoor humidity of spray chamber is maintained more than 90%, and illumination is controlled at 1500-3000lux; After 5-7 days, controlled humidity is 80-85%, and illumination is controlled at 3000-8000lux; After 5-7 days, adopt humidity 75-80%, illumination 8000-15000lux; After 30 days, can transplant to land for growing field crops.
2. a kind of hardening off method of candidum tissue culturing seedling according to claim 1, is characterized in that, described controlled light intensity is used two layers of removable shade net.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103988779A (en) * | 2014-05-22 | 2014-08-20 | 四川农业大学 | Efficient corn callus seedling regeneration culture method |
| CN104145794A (en) * | 2014-08-15 | 2014-11-19 | 湖南省农业信息与工程研究所 | Temporary planting and seedling hardening method of dendrobium tissue culture seedlings |
| CN104542209A (en) * | 2014-10-17 | 2015-04-29 | 李登云 | Cultivating and planning method for Dendrobium huoshanense seedling |
| CN104770179A (en) * | 2015-04-21 | 2015-07-15 | 李天兰 | Cultivation method for Dendrobium officinale |
| CN106332761A (en) * | 2016-08-30 | 2017-01-18 | 柳州市泓吉农业科技有限公司 | Factory cultivating method of dendrobium officinale |
| CN106359049A (en) * | 2016-08-29 | 2017-02-01 | 江西九草铁皮石斛科技协同创新有限公司 | Aerial fog type seedling hardening method of dendrobium officinale tissue culture seedlings |
| CN106416978A (en) * | 2016-09-29 | 2017-02-22 | 紫云自治县瑞生中药材种植农民专业合作社 | Method for planting dendrobium officinale |
| CN107624637A (en) * | 2017-11-08 | 2018-01-26 | 山东博华高效生态农业科技有限公司 | A kind of dendrobium candidum hardening off method |
-
2013
- 2013-12-24 CN CN201310724081.5A patent/CN103766202A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103988779A (en) * | 2014-05-22 | 2014-08-20 | 四川农业大学 | Efficient corn callus seedling regeneration culture method |
| CN104145794A (en) * | 2014-08-15 | 2014-11-19 | 湖南省农业信息与工程研究所 | Temporary planting and seedling hardening method of dendrobium tissue culture seedlings |
| CN104542209A (en) * | 2014-10-17 | 2015-04-29 | 李登云 | Cultivating and planning method for Dendrobium huoshanense seedling |
| CN104770179A (en) * | 2015-04-21 | 2015-07-15 | 李天兰 | Cultivation method for Dendrobium officinale |
| CN104770179B (en) * | 2015-04-21 | 2017-03-22 | 李天兰 | Cultivation method for Dendrobium officinale |
| CN106359049A (en) * | 2016-08-29 | 2017-02-01 | 江西九草铁皮石斛科技协同创新有限公司 | Aerial fog type seedling hardening method of dendrobium officinale tissue culture seedlings |
| CN106332761A (en) * | 2016-08-30 | 2017-01-18 | 柳州市泓吉农业科技有限公司 | Factory cultivating method of dendrobium officinale |
| CN106416978A (en) * | 2016-09-29 | 2017-02-22 | 紫云自治县瑞生中药材种植农民专业合作社 | Method for planting dendrobium officinale |
| CN107624637A (en) * | 2017-11-08 | 2018-01-26 | 山东博华高效生态农业科技有限公司 | A kind of dendrobium candidum hardening off method |
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