CN101822187A - Method for evaluating rice planthopper-resistant property of rice - Google Patents
Method for evaluating rice planthopper-resistant property of rice Download PDFInfo
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Abstract
The invention provides a method for evaluating the rice planthopper-resistant property of rice. The method includes the steps that: (1) a nylon net greenhouse is constructed; (2) rice varieties, which include a rice variety to be tested, a pest-resistant check variety and a pest-susceptible variety, are planted in the greenhouse; and the pest-susceptible variety is planted around each variety to serve as rice planthopper-inducing rows; (3) the dosage of nitrogenous fertilizer is 200kg/ha to 250kg/ha; (4) at the early tillering stage of all the varieties, 2.5 percent of deltamethrin emulsifiable concentrate or 20 percent of triazophos emulsifiable concentrate is used for killing all pests and natural enemies in the net; (5) 10 days after the step 4, adult rice planthoppers are put on the pest-susceptible check variety (TN1) planted around the rice variety to be tested, 5 to 10 pairs per clump, and are freely and naturally propagated and randomly transferred to the variety to be tested; (6) after the step 5, when all of the pest-susceptible check variety in the test field dies, the resistance of each variety is evaluated, wherein the rice variety to be tested has high resistance when the death rate of the rice variety to be tested is less than 10 percent, pest resistance when the death rate is 11 percent to 30 percent, medium resistance when the death rate is 31 percent to 50 percent, medium susceptibility when the death rate is 51 percent to 70 percent, and pest susceptibility when the death rate is higher than 70 percent. The method has the advantages of simplicity and high operability, and accords with production reality.
Description
Technical field
The present invention relates to field of pest control, provide a kind of field to identify the method for rice varieties pest resistances such as rice fulgorids.
Background technology
Delphacidae Delphacidae insect mainly contains brown planthopper Nilaparvatalugens, white-backed planthopper Sogatella furcifera and small brown rice planthopper Laodelphax striatellus in paddy ecosystem.Rice fulgorid is directly stung the phloem juice of inhaling the rice strain, cause paddy growth slowly, delays of tillering, flat grain increase.Endanger when serious, cause the rice strain withered, be " lice burning " shape.Except that directly causing harm, can also propagate virus diseases such as black streak dwarf, southern black streak dwarf and stripe virus disease, cause serious harm.Three kinds of plant hoppers alternately or break out simultaneously and cause disaster, becoming influences the of paramount importance limiting factor of China's Rice Production.The control of brown planthopper mainly depends on efficient chemical utilization of pesticides, increases the persticide residue in the paddy simultaneously by ecological problems such as increasing rampant and pesticide resistance again, influences rice quality and use chemical insecticide to cause for a long time continuously.
Utilize pest-resistant cultivar to be proved to be, economy the safest and effective measures into a kind of control brown planthopper.Domestic scholars is continued to use standard group in the seedling stage screening method (being the SSST method) founded International Rice Research Institute (IRRI) or through the improved standard of Chinese scholar group in seedling stage screening method (being the MSSST method) in decades, promptly the resistance of rice fulgorid is estimated, as rice varieties the resistance index guidance of rice fulgorid is produced with the resistance level in seedling stage at rice seedling.But many facts show, paddy rice seedling stage with become the strain phase might not be consistent to the resistance level of rice fulgorid, even seedling stage, resistance and strain-forming period resistance had than big-difference sometimes.The kind that has is bad in seedling stage performance, but becomes the resistance of strain phase to strengthen, and in resistance in seedling stage kind preferably, has arrived strain-forming period resistance and may show generally on the contrary.And these evaluations all carry out in greenhouse or cement tub, and estimating environment has big gap with the land for growing field crops, and often causing the actual resistance in its resistance level that identifies and field to show has certain deviation.Yet under natural situation, field resistance is identified and also is subjected to the influence that rice fulgorid is invaded natural calamities such as population quantity and non-target pest, differing greatly between year easily completely.The particularly important is, rice seedling rice fulgorid quantity is seldom usually becoming the strain phase just really to suffer the serious harm of rice fulgorid in process of production, therefore has only becoming the paddy rice evaluation of carrying out field against-plant hopper characteristic of strain phase can accomplish that just real the guidance produce.Given this, the field standardization is identified and evaluation for rice varieties is carried out the resistance of rice fulgorid, and we have proposed a kind of new evaluation of resistance method.
Summary of the invention
Purpose of the present invention is achieved by the following technical programs.Comprise the steps:
1. set up booth, allow the wide of booth be 6～10 meters, rise rice, along the booth longitudinal axis booth is equally divided into two, and booth is covered fully with 60-100 purpose nylon grenadine into 2-3;
2. plantation treats that rice varieties, rice varieties comprise rice varieties to be tested, pest-resistant check variety in booth; And sense worm check variety; Wherein allow the plantation on every side of rice varieties to be tested and pest-resistant check variety feel the worm check variety;
3. the nitrogenous fertilizer usage amount is 200-250kg/ha, and it is light green to be beneficial to paddy rice, increases rice fulgorid propagation;
4. kill all insects and natural enemy in the net with 2.5% deltamethrin milk oil or 20% triazophos emulsifiable solution in the early tillering stage of all kinds;
5. behind 10 days after step 4, around rice varieties to be tested, insert on the sense worm kind of plantation rice fulgorid adult 5-10 right/clump, give free rein to breeding, random migration to identification of species;
6. after step 5, when sense worm check variety is all dead in the test region, estimate each varietal resistance; When the lethality of rice varieties to be measured less than 10% be high anti-, 11-30%'s be pest-resistant, 31-50% be in anti-, 51-70%'s be middle sense, greater than 70% be to feel worm.
In above-mentioned method:
In a preferred mode, in step 1, outside booth, cover one deck 60-100 purpose nylon grenadine again, enter in the net to prevent rice fulgorid or other insect.Allow booth be in a relatively independent space like this, be consistent with natural environment again simultaneously, more help reducing the harm of extraneous non-target pest, can control the quantity of rice fulgorid simultaneously, it is realistic to obtain resistant variety like this, and identifies the efficient height.
In a preferred mode, in step 2, the sub-district area of each kind is 5～15m
2, various 10-15 are capable for each kind, plant 2 row sense worm check varieties around each kind, are beneficial to bring out rice fulgorid, and line space is 20 * 20cm.
In a preferred mode, in step 2, pest-resistant check variety is IR26 and IR36, and sense worm check variety is TN1, is accepted standard kind in the industry, obtains easily.
The present invention also provides a kind of method, comprising:
1, the paddy field easily of selecting draining and irrigation all to compare, build 6～10 meters wide steel pipe booth, rise 2-3 rice, long is 80-100 rice, build a ridge that 30cm is wide in the centre of canopy, booth is divided into two, whole booth covers with 60-100 purpose nylon grenadine, canopy outdoors face refill one deck nylon yarn, enter in the net to prevent rice fulgorid or other insect;
2, set up the sub-district according to rice varieties quantity to be measured, every sub-district area 5～15m
2To each kind soak seed, after the operations such as vernalization, sowing, transplanting; Comprise pest-resistant check variety IR26 and IR36, sense worm check variety is that TN1 and rice varieties to be measured are planted in each sub-district, and various 10-15 are capable for each kind, around each rice varieties to be measured and pest-resistant check variety, plant 2 row TN1, be beneficial to bring out brown planthopper, line space is 20 * 20cm;
3, the nitrogenous fertilizer usage amount is 200-250kg/ha, and it is light green to be beneficial to paddy rice, quickens rice fulgorid propagation;
4, the early tillering stage is killed all insects and natural enemy in the net with 2.5% deltamethrin milk oil or 20% triazophos emulsifiable solution; After the medication 10 days, insert on the TN1 of plantation around the rice varieties to be measured rice fulgorid adult 5-10 right/clump, give free rein to breeding, random migration to identification of species;
5, connect and when test region contrast sense worm kind TN1 is all dead, estimate each varietal resistance behind the worm; Wherein, the lethality of surveying rice varieties the present age less than 10% be high anti-, 11-30%'s be pest-resistant, 31-50% be in anti-, 51-70%'s is middle sense, greater than 70% be to feel worm.
Beneficial effect
By the enforcement of this scheme, obtained following beneficial effect:
(1) existing resistance authentication method does not provide the method that the paddy rice one-tenth strain phase is estimated the resistance of rice fulgorid under the environment of field, and enforcement of the present invention can become the actual resistance performance to brown planthopper in the field of strain phase by clear and definite rice varieties, and method is easy, workable, meet and produce reality.
(2) utilize enforcement of the present invention, when resistance is identified, be not vulnerable to the influence of factors such as rice fulgorid abiogenesis degree, weather, can guarantee test carry out smoothly, and good reproducibility, the resistance result of acquisition is more reliable.
(3) the field rice fulgorid often is that brown planthopper and white-backed planthopper mix generation, under natural situation, is difficult to accurately distinguish the resistance of rice varieties to brown planthopper or white-backed planthopper.Utilize enforcement of the present invention, can effectively guarantee the unicity in worm source.
Embodiment
Embodiment 1 solarium makes up and the sub-district design
Select draining and irrigate the paddy field easily that all compares, adopt associating eight type monomer booths, wide 8 meters, long 80 meters, 0.6 meter of tube pitch is risen 2.5 meters, 1.5 meters of shoulder heights.A ridge that 30cm is wide is built in the centre, and booth is divided into two.Booth covers with 80 purpose nylon grenadines, canopy outdoors face refill one deck 80 orders, than equal nylon yarn of wide 30cm around the canopy door, the ground connection place uses the clod compacting, enters in the net to prevent rice fulgorid or other insect.
Set up the sub-district according to kind quantity, average every cell-wide is 2 meters.Operations such as seed soaking, vernalization, sowing, transplanting are all carried out routinely.Single this transplanting of hybrid rice kind, two these transplantings of japonica rice variety.Various 10 row of each kind (comprise pest-resistant check variety IR26 and IR36, sense worm check variety is TN1) are planted 2 row TN1 around each kind, in order to bringing out of brown planthopper, line space is 20 * 20cm.Each kind repeats randomized arrangement 3 times.The nitrogenous fertilizer usage amount is 250kg/ha.
Early tillering stage is killed all insects and natural enemy in the net with 500 times of sprayings of 20% triazophos emulsifiable solution.After the medication 10 days, on TN1, insert 5 pairs/clump of rice fulgorid adults, give free rein to breeding, random migration to identification of species.
When the TN1 kind is all dead, estimate each varietal resistance.Lethality less than 10% be high anti-(HR), that 11-30% be pest-resistant (R), 31-50% be in anti-(MR), that 51-70% be middle sense (MS), greater than 70% be to feel worm (S).The results are shown in Table 1, identify 6 parts of high anti-kinds (being), 15 parts of resistant varieties (being), in 11 parts of anti-kinds (being).
The different rice varieties of table 1 (being) show and hold anti-characteristic to the field resistance of brown planthopper
The different fertilizer level of embodiment 2 is to the influence of rice varieties resistance to insects
Solarium with embodiment 1 is made measures kind TN1, IR26 and IR36 resistance to brown planthopper under different nitrogenous fertilizer levels.When nitrogenous fertilizer uses to 250kg/ha, IR26 and IR36 to the resistance level of brown planthopper be respectively the sense worm and in anti-, and when not using nitrogenous fertilizer, sense and pest-resistant during both resistance levels are respectively.Sense worm kind TN1 is not subjected to the influence (table 2) of fertilizer level.
Under the different nitrogenous fertilizer levels of table 2 rice varieties to the resistance of brown planthopper
Under the embodiment 3 abiogenesis situations and utilize the inventive method to identify the comparison of paddy rice resistance to insects
Respectively in the abiogenous situation of brown planthopper with utilize enforcement of the present invention, monitoring kind TN1, IR26 and IR36 are to the resistance of brown planthopper, and the result shows that IR26 is 9 grades to the brown planthopper resistance, TN1 is identical with sense worm kind, shows that IR26 has lost resistance fully to brown planthopper.The resistance of IR36 be 3 grades a little more than 5 grades under the abiogenesis situation, show IR36 brown planthopper is still had in anti-level.Illustrate and adopt method of the present invention and the result's consistent (table 3) who under natural situation, identifies the paddy rice resistance to insects, but under natural situation, identify the influence that is subject to insect occurrence degree and natural calamity, and adopt not susceptible to of method of the present invention, more stable between year, experimental repeatability is good.
Table 3 utilizes the inventive method and identify the resistance result of rice varieties to brown planthopper under natural situation
Embodiment 4 differences connect the influence of worm amount to the paddy rice resistance to insects
2 solariums that built by embodiment 1, wherein connecing the brown planthopper adult in 1 solarium is 5 pairs/clump, connects 10 pairs/clump in 1 solarium in addition.The result shows that the lethality of each kind paddy rice seedling is higher than the lethality of hanging down under the worm amount condition under the high worm amount condition when TN1 is dead.Resistance level slightly descends, but general trend does not change yet.Illustrate where necessary, can strengthen and connect the worm amount, shorten qualification time, raise the efficiency.
Table 4 difference connects the influence of worm amount to the rice varieties brown planthopper resistant
Claims (7)
1. the method for a field evaluation paddy rice against-plant hopper characteristic is characterized in that this method comprises:
(1), sets up booth, allow the wide of booth be 6～10 meters, rise rice, along the booth longitudinal axis booth is equally divided into two, and booth is covered fully with 60-100 purpose nylon grenadine into 2-3;
(2), rice cultivation kind in booth, rice varieties comprises rice varieties to be tested, pest-resistant check variety; And sense worm check variety; Wherein, plantation sense worm check variety around rice varieties to be tested and pest-resistant check variety;
(3), the nitrogenous fertilizer usage amount is 200-250kg/ha, and it is light green to be beneficial to paddy rice, quickens rice fulgorid propagation;
(4), kill all insects and natural enemy in the net with 2.5% deltamethrin milk oil or 20% triazophos emulsifiable solution in the early tillering stage of all kinds;
(5), behind 10 days of step 4, insert on the sense worm check variety of plantation around the rice varieties to be tested rice fulgorid adult 5-10 right/clump, the breeding of giving free rein to, random migration are on kind to be tested;
(6), after step 5, when sense worm check variety is all dead in the test region, estimate each varietal resistance; Wherein, when the lethality of rice varieties to be measured less than 10% be high anti-, 11-30%'s be pest-resistant, 31-50% be in anti-, 51-70%'s be middle sense, greater than 70% be to feel worm.
2. method according to claim 1 is characterized in that, in step 1, covers one deck 60-100 purpose nylon grenadine again outside booth, to prevent in rice fulgorid or other insect turnover net.
3. method according to claim 2 is characterized in that, in step 2, the sub-district area of each kind is 5～15m
2, various 10-15 are capable for each kind, plant 2 row sense worm check varieties around each rice varieties to be tested and pest-resistant check variety, are beneficial to bring out brown planthopper, and line space is 20 * 20cm.
4. method according to claim 3 is characterized in that, described pest-resistant check variety is IR26 or IR36, and sense worm check variety is TN1.
5. method according to claim 3 is characterized in that, described pest-resistant check variety is IR36, and sense worm check variety is TN1.
6. method according to claim 3 is characterized in that, the nitrogenous fertilizer usage amount is 250kg/ha.
7. method according to claim 1 is characterized in that, described two-layer nylon grenadine is 80 orders.
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| CN2010101627572A CN101822187B (en) | 2010-04-30 | 2010-04-30 | Method for evaluating rice planthopper-resistant property of rice |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102577897A (en) * | 2012-02-16 | 2012-07-18 | 扬州大学 | Method for ecological safety evaluation on nilaparvata lugens by Bt genetically modified rice based on intervention of pesticides |
| CN103749230A (en) * | 2014-01-27 | 2014-04-30 | 江苏徐淮地区连云港农业科学研究所 | Anti-rice black-streaked dwarf virus field inoculation identification method for rice varieties |
| CN103782966A (en) * | 2014-02-13 | 2014-05-14 | 云南省农业科学院生物技术与种质资源研究所 | Method for identifying rice planthopper resistance of rice |
| CN105165502A (en) * | 2015-07-21 | 2015-12-23 | 中国水稻研究所 | Method for evaluating resistance of rice in adult-plant stage against sogatella furcifera under semi-field conditions |
| CN106489564A (en) * | 2015-09-08 | 2017-03-15 | 无锡南理工科技发展有限公司 | A kind of Rice Resistance tingia dwarfing authentication method |
| CN106997563A (en) * | 2017-04-14 | 2017-08-01 | 江西省农业科学院植物保护研究所 | A kind of method for evaluating paddy ecosystem stability |
| CN108184578A (en) * | 2017-12-22 | 2018-06-22 | 武汉禾泰青生物科技有限公司 | A kind of identification method of rice brown planthopper resistant |
| CN108496724A (en) * | 2018-04-08 | 2018-09-07 | 四川省农业科学院水稻高粱研究所 | Rice-stem borer, the foundation of yellow rice borer and rice leaf roller screening and identification garden and maintaining method |
| CN108633529A (en) * | 2018-05-14 | 2018-10-12 | 四川农业大学 | It is a kind of based on the rice of radar map to the evaluation of resistance method of planthopper |
| CN108812125A (en) * | 2018-06-12 | 2018-11-16 | 广西大学 | A kind of anti-accurate identification method of brown plant-hopper scale of rice seedling |
| CN109328929A (en) * | 2018-11-01 | 2019-02-15 | 江西省农业科学院水稻研究所 | A kind of method of Natural infection and the anti-snout moth's larva rice material of screening |
| CN110178670A (en) * | 2019-06-19 | 2019-08-30 | 武汉禾泰青生物科技有限公司 | A kind of identification method of rice to brown paddy plant hopper tolerance |
| CN111264320A (en) * | 2020-03-06 | 2020-06-12 | 湖南杂交水稻研究中心 | Method for accurately identifying resistance of brown planthopper in adult stage of rice |
| CN114271158A (en) * | 2021-12-30 | 2022-04-05 | 华智生物技术有限公司 | Method for identifying resistance of brown planthopper of rice in field adult stage |
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2010
- 2010-04-30 CN CN2010101627572A patent/CN101822187B/en not_active Expired - Fee Related
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| CN102577897A (en) * | 2012-02-16 | 2012-07-18 | 扬州大学 | Method for ecological safety evaluation on nilaparvata lugens by Bt genetically modified rice based on intervention of pesticides |
| CN103749230A (en) * | 2014-01-27 | 2014-04-30 | 江苏徐淮地区连云港农业科学研究所 | Anti-rice black-streaked dwarf virus field inoculation identification method for rice varieties |
| CN103782966A (en) * | 2014-02-13 | 2014-05-14 | 云南省农业科学院生物技术与种质资源研究所 | Method for identifying rice planthopper resistance of rice |
| CN103782966B (en) * | 2014-02-13 | 2015-06-10 | 云南省农业科学院生物技术与种质资源研究所 | Method for identifying rice planthopper resistance of rice |
| CN105165502A (en) * | 2015-07-21 | 2015-12-23 | 中国水稻研究所 | Method for evaluating resistance of rice in adult-plant stage against sogatella furcifera under semi-field conditions |
| CN105165502B (en) * | 2015-07-21 | 2017-11-07 | 中国水稻研究所 | A kind of method of half field evaluation paddy rice Adult plant whitebacked planthopper-resistance |
| CN106489564A (en) * | 2015-09-08 | 2017-03-15 | 无锡南理工科技发展有限公司 | A kind of Rice Resistance tingia dwarfing authentication method |
| CN106997563A (en) * | 2017-04-14 | 2017-08-01 | 江西省农业科学院植物保护研究所 | A kind of method for evaluating paddy ecosystem stability |
| CN108184578A (en) * | 2017-12-22 | 2018-06-22 | 武汉禾泰青生物科技有限公司 | A kind of identification method of rice brown planthopper resistant |
| CN108184578B (en) * | 2017-12-22 | 2021-01-05 | 武汉禾泰青生物科技有限公司 | Identification method for brown planthopper resistance of rice |
| CN108496724B (en) * | 2018-04-08 | 2020-04-21 | 四川省农业科学院水稻高粱研究所 | Method for establishing and maintaining rice stem borer, rice stem borer and rice leaf roller screening and identifying garden |
| CN108496724A (en) * | 2018-04-08 | 2018-09-07 | 四川省农业科学院水稻高粱研究所 | Rice-stem borer, the foundation of yellow rice borer and rice leaf roller screening and identification garden and maintaining method |
| CN108633529A (en) * | 2018-05-14 | 2018-10-12 | 四川农业大学 | It is a kind of based on the rice of radar map to the evaluation of resistance method of planthopper |
| CN108812125A (en) * | 2018-06-12 | 2018-11-16 | 广西大学 | A kind of anti-accurate identification method of brown plant-hopper scale of rice seedling |
| CN109328929A (en) * | 2018-11-01 | 2019-02-15 | 江西省农业科学院水稻研究所 | A kind of method of Natural infection and the anti-snout moth's larva rice material of screening |
| CN109328929B (en) * | 2018-11-01 | 2021-06-18 | 江西省农业科学院水稻研究所 | Method for naturally identifying and screening snout moth's larva resistant rice material in field |
| CN110178670A (en) * | 2019-06-19 | 2019-08-30 | 武汉禾泰青生物科技有限公司 | A kind of identification method of rice to brown paddy plant hopper tolerance |
| CN111264320A (en) * | 2020-03-06 | 2020-06-12 | 湖南杂交水稻研究中心 | Method for accurately identifying resistance of brown planthopper in adult stage of rice |
| CN114271158A (en) * | 2021-12-30 | 2022-04-05 | 华智生物技术有限公司 | Method for identifying resistance of brown planthopper of rice in field adult stage |
| CN114271158B (en) * | 2021-12-30 | 2022-12-23 | 华智生物技术有限公司 | Method for identifying resistance of brown planthopper of rice in field adult stage |
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