CN105165502B - A kind of method of half field evaluation paddy rice Adult plant whitebacked planthopper-resistance - Google Patents
A kind of method of half field evaluation paddy rice Adult plant whitebacked planthopper-resistance Download PDFInfo
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- CN105165502B CN105165502B CN201510430004.8A CN201510430004A CN105165502B CN 105165502 B CN105165502 B CN 105165502B CN 201510430004 A CN201510430004 A CN 201510430004A CN 105165502 B CN105165502 B CN 105165502B
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- 241000196324 Embryophyta Species 0.000 title claims abstract description 48
- 238000011156 evaluation Methods 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 25
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 91
- 241001498622 Cixius wagneri Species 0.000 claims abstract description 32
- 239000004568 cement Substances 0.000 claims abstract description 23
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 19
- 241000176086 Sogatella furcifera Species 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000012512 characterization method Methods 0.000 claims abstract description 8
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- 241001466044 Delphacidae Species 0.000 description 2
- 241000258937 Hemiptera Species 0.000 description 2
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- 238000009395 breeding Methods 0.000 description 2
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- 235000013339 cereals Nutrition 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 239000002917 insecticide Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
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- 241000238631 Hexapoda Species 0.000 description 1
- 241000500891 Insecta Species 0.000 description 1
- 241001470017 Laodelphax striatella Species 0.000 description 1
- 241001556089 Nilaparvata lugens Species 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Biodiversity & Conservation Biology (AREA)
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Abstract
The invention discloses a kind of method of half field evaluation paddy rice Adult plant whitebacked planthopper-resistance, the rice cultivation in cement pit, the paddy rice includes rice varieties to be measured, pest-resistant check variety and sense worm check variety, the rice varieties to be measured and pest-resistant check variety surrounding plantation sense worm check variety, normal water and fertilizer management；At rice tillering initial stage, 1 pair of emerging adult, natural propagation at the beginning of connecing white backed planthopper with every tiller；According to the anti-white backed planthopper characterization and evaluation standard of rice varieties Adult plant, start to evaluate the resistance of rice varieties to be measured when feeling worm check variety and reaching 7 grades, until sense worm check variety is all dead, the resistance of rice varieties to be measured is final resistance when sense worm check variety is all dead；The present invention overcomes can not carry out the problem of Adult plant against-plant hopper is identified to batch rice varieties in the prior art, compensate for only carrying out the deficiency of anti-white backed planthopper evaluation with seedling stage assay.
Description
(1) technical field
The present invention relates to a kind of Rice Resistance white backed planthopper evaluating characteristics, more particularly under controlled condition, pass through water
The Resistance Identification of rice Adult plant Whitebacked Planthopper, improves Rice Resistance white backed planthopper identification technology level.
(2) background technology
Planthopper is Insecta, Homoptera (Homoptera), Delphacidae (Delphacidae) insect.Plant juice is inhaled to pierce
The crops such as liquid hazard rice, are the primary pests in Rice Production.Frequent species have brown paddy plant hopper (Nilaparvata lugens),
White backed planthopper (Sogatella furcifera) and small brown rice planthopper (Laodelphax striatellus).Planthopper generally lives away from home
It is main to draw paddy rice juice in paddy rice basal part of stem or fringe portion, in paddy growth early stage, cause that paddy growth is slow, tiller delay；
In Rise's boot period, performance is not eared when endangering serious；In Grain Filling, then mass of 1000 kernel is caused to mitigate, rate of immatured grain increase, sternly
The underproduction or withered again.In addition to directly endangering, planthopper causes rice disease with viruses such as stripe virus disease, black streak dwarf
Prevalence.The preventing and treating of planthopper relies primarily on efficient chemical insecticide, and chemical insecticide, which is used for a long time, can cause plant hopper again
The rampant and resistance to the action of a drug, while pesticide residues in rice is caused, so as to influence rice quality.Selection utilization has relatively strong anti-to planthopper
Property rice varieties be preventing and treating the most safe and effective method of plant hopper.There is seedling stage mirror the country in Resistance of Rice Varieties evaluation at present
Determine method and Adult plant field test method.Continue to use the standard seedling stage group screening method of International Rice Research Institute's foundation always for seedling stage
(SSST methods) or through the improved standard seedling screening method of Chinese scholar (MSST methods), i.e., the resistance of planthopper is entered in rice seedling
Row is evaluated, using the resistance level in seedling stage as paddy rice to planthopper Resistant index Instructing manufacture.The experiment table of this group in recent years
Bright, many rice varieties are inconsistent to the resistance level of planthopper in seedling stage and Adult plant, and some even have very big difference.Have
Kind it is bad in wheat seeding, but may increase in the resistance of Adult plant, it is conversely also right.Recent year scholar invents
Using paddy rice Adult plant as identification target, against-plant hopper authentication method is carried out only by evaluation criterion of the rice varieties death rate, is made up
The deficiency of against-plant hopper evaluation is only carried out with seedling stage assay, still, such a identification is used for the identification of high-volume rice varieties
Method is, it is necessary to which the planthopper of sufficient amount is difficult.In consideration of it, in order to which batch rice varieties carry out Adult plant against-plant hopper identification, I
Invented a kind of new anti-white backed planthopper evaluation criterion in half field.
(3) content of the invention
It is an object of the present invention to provide a kind of evaluation method of the anti-whitebacked planthopper-resistance of half field rice Adult plant, overcome existing
The problem of Adult plant against-plant hopper is identified can not be carried out to batch rice varieties, compensate for only with seedling stage assay being resisted in technology
The deficiency that white backed planthopper is evaluated.
The technical solution adopted by the present invention is：
The present invention provides a kind of method of half field evaluation paddy rice Adult plant whitebacked planthopper-resistance, and described method is：(1)
Cement pit (the cement pit pond along be provided with groove) is set up, and above cement pit builds framework (framework can be rectangular
Build, or shed type), cover nylon grenadine cover on the framework, the nylon wire screen covering point top subnetting yarn and under
Part grenadine, the top subnetting yarn leaves extension, and the top subnetting yarn is provided with and can lived provided with sample mouthful, the sample mouthful
First lock of dynamic connection；The bottom of the bottom subnetting yarn is fixed on cement pit edge and (is fixed on the recessed of cement pit pond edge
In groove), the bottom subnetting yarn top is connected with top subnetting yarn by the second lock of movable connection, and the extension covers
Cover bottom subnetting yarn outer surface (preferably extension grenadine is highly the 1/2~1 of bottom subnetting yarn height)；(2) in cement pit
Interior rice cultivation, the paddy rice includes rice varieties to be measured, pest-resistant check variety and sense worm check variety, the paddy rice product to be measured
Kind and pest-resistant check variety surrounding plantation sense worm check variety (rice varieties to be measured and pest-resistant check variety are planted at random, repeat 3
It is secondary, often repeat 3 caves of plantation), normal water and fertilizer management；The sense worm check variety is TN1；The pest-resistant check variety is IR56；
(3) in rice tillering initial stage (1~2 healthy and strong tiller is stayed per cave), 1 pair of emerging adult at the beginning of connecing white backed planthopper with every tiller is natural
Breeding；(4) according to the anti-white backed planthopper characterization and evaluation standard of rice varieties Adult plant, start when feeling worm check variety and reaching 7 grades
The resistance of rice varieties to be measured is evaluated, until sense worm check variety is all dead, product to be tested when sense worm check variety is all dead
The resistance planted is final resistance；
The anti-white backed planthopper characterization and evaluation standard of rice varieties Adult plant is：
0 grade：Plant is normal, no plant hopper Damage pattern；Resistance level is high anti-；
1 grade：Plant and solid normal, has slight Damage pattern, base portion is without obvious nigrescence；Resistance level is pest-resistant；
3 grades：Plant dwarfing is not obvious, and setting percentage is low；Bottom leaf sheath blacks, and has obvious plant hopper Damage pattern；Resistance level is
Moderate resistance；
5 grades：Plant has been downgraded or turned to be yellow, and ears normal, but diminish；Middle and lower part leaf sheath blacks；Resistance level is
Middle sense；
7 grades：Plant is substantially downgraded, and heading is postponed, and fringe is substantially small；Middle and lower part blade blacks, withered；Resistance level is sense
Worm；
9 grades：Plant is near withered or does not ear；Complete stool major part blade blacks；Resistance level is felt to be high.
8 meters of cement pit length of the present invention, it is wide 1.7 meters, it is high 0.8 meter, build high 1.5 meters of steelframes along cement pit surrounding
40 mesh nylon wire screen coverings are covered on frame, the steelframe frame.
Rice varieties to be measured and pest-resistant check variety of the present invention, sense worm check variety each plant 3 caves, are repeated 3 times,
Per cave Hybrid Rice Varieties 1~2, conventional rice variety 2~3,15 × 20cm of seeding row spacing.
Nylon wire screen covering top subnetting yarn of the present invention is with bottom subnetting yarn highly than being 3:1 (preferred bottom subnetting yarn
It is 0.5 meter high), the sample mouthful is perpendicular to cement pit edge.
Part top to bottom fills 1 sample mouthful every 2 meter amperes on nylon wire screen covering of the present invention, and the sample mouthful is prolonged
Reach extension bottom (the sample mouthful length is the 3/4~1 of steelframe height).First lock is slide fastener, described second
Latch velcro.
The method of half field evaluation paddy rice Adult plant whitebacked planthopper-resistance of the present invention weight when being evaluated and tested to rice varieties
It is multiple to carry out, take the mean as resistance class, grade is determined referring in particular to table 2：
The average aggrieved grade between repeating of table 2
Compared with prior art, the beneficial effects are mainly as follows：The present invention provide a kind of half field rice into
The evaluation method of strain phase whitebacked planthopper-resistance, the framework covered with nylon wire upper cover, and nylon grenadine are built in cement pit surrounding
Two parts above and below cover point, lower part is fixed, and sample mouthful is set up on top separately, conveniently looks into worm, avoids white backed planthopper from flying out while avoiding,
Improve the reliability of inspection；The present invention overcomes can not carry out Adult plant against-plant hopper mirror to batch rice varieties in the prior art
The problem of determining, compensate for the deficiency only evaluated with the anti-white backed planthopper of seedling stage assay.
(4) illustrate
Fig. 1 is nylon wire screen covering front schematic view, and 1- bottoms subnetting yarn, 2- tops subnetting yarn, 3- second is latched, 4- samples
Mouthful, the upper part extension bottoms of 5-, 6- tops subnetting yarn extension.
The half field photo that Fig. 2 identifies for the present invention for whitebacked planthopper-resistance.
The half field side photo that Fig. 3 identifies for the present invention for whitebacked planthopper-resistance.
(5) embodiment
With reference to specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in
This：
Embodiment 1：The identification of rice varieties Adult plant Whitebacked Planthopper resistance under half field condition
1) reference picture 1- Fig. 3, sets up cement pit, and cement pit pond is wide 1.7 meters along groove, 8 meters of cement pit length is provided with, high by 0.8
Rice, the high 1.5 meters of steelframe frames of cement pit restocking, and the 40 mesh nylon wire screen covering in steelframe frame upper cover.The upper part of guard point and bottom
Point, top fractionation has extension, and upper part is high 1.3 meters, and extension is high 0.2 meter；High 0.5 meter or so of bottom subnetting yarn, lower part
Grenadine bottom is compacted in the groove at cement pit edge, prevents planthopper from climbing out of；Bottom subnetting yarn top by velcro with
Top subnetting yarn connection, the extension of top subnetting yarn is covered in bottom subnetting yarn outer surface；Along upper operative tip to extension
1 meter of long slide fastener is installed in bottom direction, and slide fastener and cement pit pond are along vertical or with the highly consistent slide fastener of steelframe frame, general
Lower part is completely covered, and fills one at interval of 2 meter amperes, conveniently looks into worm.
2) sowing (can also use transplanting method, seed soaking, vernalization, sowing, transplanting etc. is operated routinely using point broadcast mode
Carry out).The rice cultivation kind in pond, including rice varieties to be measured (11), pest-resistant check variety (IR56), sense worm reference substance
Plant (TN1), rice varieties to be measured and pest-resistant check variety are planted at random, in kind to be measured, anti-sense worm check variety periphery
Plantation sense worm check variety, is repeated 3 times per kind, and 3 caves of plantation are repeated every time.Rice varieties to be measured and anti-, sense check variety are every
Bunch planting kind 3~5 vernalization seed, 15 × 20cm of seeding row spacing, normal water and fertilizer management.
3) treat that 10 days or so the length of time rice seedlings grow rejected weak seedling, healthy seedling 1~2 (hybrid paddy rice 1, conventional Rice 2) is stayed per cave, in water
The rice early tillering stage, 1~2 healthy and strong tiller is stayed per cave, just 1 pair of emerging adult is connect with every tiller, breeding is allowed to.
4) according to the anti-white backed planthopper characterization and evaluation standard of rice varieties Adult plant, when sense worm check variety TN1 reaches 7 grades
When, start to evaluate the resistance of kind to be measured by the anti-white backed planthopper characterization and evaluation standard of rice varieties Adult plant, until sense worm control
It is all dead.The resistance for feeling kind to be measured when worm compares all dead is final resistance.
The anti-white backed planthopper characterization and evaluation standard of the rice varieties Adult plant of table 1
In triplicate, when calculating average aggrieved grade between repeating, handled by table 2：
The average aggrieved grade between repeating of table 2
Qualification result is obtained according to above method be shown in Table identify Adult plant Whitebacked Planthopper altogether in 3,11 portions of paddy rice and have
2 parts of the kind (river section 736, magnificent 3084S) of moderate resistance, middle 4 parts of kind of sense (magnificent 1165S, spring excellent 84, Luo You 9348 and elegant water 134).
Table 3：Different rice varieties (are) resistance of Whitebacked Planthopper
| Variety name | Anti- level | Resistance level |
| River section 736 | 3 | MR |
| Magnificent 3084S | 3 | MR |
| Magnificent 1165S | 5 | MS |
| Spring excellent 84 | 5 | MS |
| Luo You 9348 | 5 | MS |
| Elegant water 134 | 5 | MS |
| Provide 100B | 7 | S |
| Y two excellent 1 | 7 | S |
| Guangzhan 63S -4 | 7 | S |
| Excellent No. 1 of strain two | 9 | HS |
| 7011 | 9 | HS |
| IR56 | 1 | HR |
| TN1 | 9 | HS |
Embodiment 2：Near-isogenic rice lines compare in the case of half field and field natural INFESTATION
The Resistance Identification be the same as Example 1 of paddy rice Whitebacked Planthopper under half field condition, rice varieties (8) to be measured are shown in Table
4。
The Resistance Identification of field natural INFESTATION Rice under Condition Whitebacked Planthopper：
(1) Quarter Design：Average every 5.4 × 2 meters of cell.The operation such as seed soaking, vernalization, sowing, transplanting is routinely carried out,
2 kinds to be measured, 20 × 25cm of seeding row spacing are planted per cell.This transplanting of hybrid paddy rice list, 2~3 transplantings of conventional Rice.Each kind
Between (including kind to be measured, pest-resistant control IR56) each 3 row, the often cave of row 27, two kinds to be measured or kind to be measured with it is pest-resistant right
According to 1 row TN1 is planted between kind, while planting 2 row TN1 in kind to be measured and pest-resistant check variety periphery.Each kind
It is repeated 3 times, random alignment.
(2) normal water and fertilizer management, product to be tested kind, evaluation criterion be the same as Example 1 are evaluated when feeling worm and compareing 7 grades.
(3) field natural INFESTATION, investigation finds that planthopper is caused harm and caused harm to be comprehensive, early stage based on white backed planthopper, after
Phase based on brown paddy plant hopper, the results are shown in Table 4.As a result show according to the authentication method of the present invention and criterion to the white backward flight of Rice Resistance
The qualification result of lice and field natural INFESTATION result are basically identical, it was demonstrated that this method qualification result is reliable.
Table 4：Half field Adult plant and the resistance result of Rice Varieties plant hopper in the case of natural INFESTATION
Claims (6)
1. a kind of method of half field evaluation paddy rice Adult plant whitebacked planthopper-resistance, it is characterised in that described method is：(1) build
Vertical cement pit, and built above cement pit on framework, the framework covered with nylon grenadine cover；On the nylon wire screen covering point
Part grenadine and bottom subnetting yarn, the top subnetting yarn leave extension, and the top subnetting yarn is provided with sample mouthful, the inspection
First lock of the sample mouthful provided with movable connection；The bottom of the bottom subnetting yarn is fixed on cement pit edge, the lower part
Grenadine top is connected with top subnetting yarn by the second lock of movable connection, and the extension is covered in outside the subnetting yarn of bottom
Surface；(2) rice cultivation in cement pit, the paddy rice includes rice varieties to be measured, pest-resistant check variety and sense worm reference substance
Kind, the rice varieties to be measured and pest-resistant check variety surrounding plantation sense worm check variety, normal water and fertilizer management；The sense worm pair
It is TN1 according to kind；The pest-resistant check variety is IR56；(3) at rice tillering initial stage, sprout wings at the beginning of connecing white backed planthopper with every tiller
1 pair of adult, natural propagation；(4) according to the anti-white backed planthopper characterization and evaluation standard of rice varieties Adult plant, when sense worm check variety reaches
Start to evaluate the resistance of rice varieties to be measured during to 7 grades, until sense worm check variety is all dead, sense worm check variety is all dead
The resistance of rice varieties to be measured is final resistance when dying；
The anti-white backed planthopper characterization and evaluation standard of rice varieties Adult plant is：
0 grade：Plant is normal, no plant hopper Damage pattern；Resistance level is high anti-；
1 grade：Plant and solid normal, has slight Damage pattern, base portion is without obvious nigrescence；Resistance level is pest-resistant；
3 grades：Plant dwarfing is not obvious, and setting percentage is low；Bottom leaf sheath blacks, and has obvious plant hopper Damage pattern；Resistance level is moderate resistance；
5 grades：Plant has been downgraded or turned to be yellow, and ears normal, but diminish；Middle and lower part leaf sheath blacks；Resistance level is middle sense；
7 grades：Plant is substantially downgraded, and heading is postponed, and fringe is substantially small；Middle and lower part blade blacks, withered；Resistance level is sense worm；
9 grades：The tiller of plant flap≤1/ is not eared；Complete stool major part blade blacks；Resistance level is felt to be high.
2. the method for half field evaluation paddy rice Adult plant whitebacked planthopper-resistance as claimed in claim 1, it is characterised in that the water
8 meters of mud sump length, it is wide 1.7 meters, it is high 0.8 meter, built along cement pit surrounding and covered 40 on high 1.5 meters of steelframe frames, the steelframe frame
Mesh nylon wire screen covering.
3. the method for half field evaluation paddy rice Adult plant whitebacked planthopper-resistance as claimed in claim 1, it is characterised in that described to treat
Survey rice varieties and pest-resistant check variety, sense worm check variety plant 3 caves respectively, be repeated 3 times, per cave Hybrid Rice Varieties 1~2
Originally, conventional rice variety 2~3,15 × 20cm of seeding row spacing.
4. the method for half field evaluation paddy rice Adult plant whitebacked planthopper-resistance as claimed in claim 2, it is characterised in that the Buddhist nun
Imperial grenadine cover top subnetting yarn is with bottom subnetting yarn highly than being 3:1, the sample mouthful is perpendicular to cement pit.
5. the method for half field evaluation paddy rice Adult plant whitebacked planthopper-resistance as claimed in claim 4, it is characterised in that on described
Part grenadine circumferentially sets 1 sample mouthful every 2 meters, and the sample mouthful extends to extension bottom.
6. the method for half field evaluation paddy rice Adult plant whitebacked planthopper-resistance as claimed in claim 4, it is characterised in that described
One lock is slide fastener, and second lock is velcro.
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| CN108812125A (en) * | 2018-06-12 | 2018-11-16 | 广西大学 | A kind of anti-accurate identification method of brown plant-hopper scale of rice seedling |
| CN111557215A (en) * | 2020-02-27 | 2020-08-21 | 四川省农业科学院植物保护研究所 | Identification method and application of rice variety resisting plant hopper and SRBSDV (sequence-specific locus-resistant virus) in adult stage |
| CN111264320A (en) * | 2020-03-06 | 2020-06-12 | 湖南杂交水稻研究中心 | Method for accurately identifying resistance of brown planthopper in adult stage of rice |
| CN112243812A (en) * | 2020-08-27 | 2021-01-22 | 河南科技大学 | Wheat growth period field aphid-resistant screening and identifying device |
| CN112106733B (en) * | 2020-09-18 | 2022-03-25 | 中国水稻研究所 | Indoor biological assay method for rice planthopper |
| CN114271158B (en) * | 2021-12-30 | 2022-12-23 | 华智生物技术有限公司 | Method for identifying resistance of brown planthopper of rice in field adult stage |
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